DGKG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKG
Entrez Gene ID	1608
Full Name	diacylglycerol kinase gamma
Synonyms	DAGK3,DGK-GAMMA
General protein information	Preferred Names diacylglycerol kinase gamma Names diacylglycerol kinase gamma DAG kinase gamma diacylglycerol kinase, gamma 90kDa diacylglyerol kinase gamma diglyceride kinase gamma
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q27.2-q27.3
Summary	This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601854

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080745.2	NP_001074214.1	diacylglycerol kinase gamma isoform 3
NM_001346.2	NP_001337.2	diacylglycerol kinase gamma isoform 1
NM_001080745.1	NP_001074214.1	diacylglycerol kinase gamma isoform 3
NM_001346.3	NP_001337.2	diacylglycerol kinase gamma isoform 1
NM_001080744.2	NP_001074213.1	diacylglycerol kinase gamma isoform 2
NM_001080744.1	NP_001074213.1	diacylglycerol kinase gamma isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DGKG	XP_545239.2
Rattus norvegicus (Norway rat)	Dgkg	NP_037258.1
Pan troglodytes (chimpanzee)	DGKG	XP_001152821.1
	DGKG	XP_001092912.1
Danio rerio (zebrafish)	LOC567728	XP_696120.6
Homo sapiens (human)	DGKG	NP_001337.2
Bos taurus (cattle)	DGKG	XP_002684915.2
Mus musculus (house mouse)	Dgkg	NP_619591.1
Gallus gallus (chicken)	DGKG	XP_422650.4
Oryza sativa (rice)	Os03g0425300	NP_001173480.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.
Kai M, Yamamoto E, Sato A, Yamano HO, Niinuma T, Kitajima H, Harada T, Aoki H, Maruyama R, Toyota M, Hatahira T, Nakase H, Sugai T, Yamashita T, Toyota M, Suzuki H
Molecular carcinogenesis56(7)1743-1752(2017 Jul)

Diacylglycerol kinase ? is a novel anionic phospholipid binding protein with a selective binding preference.
Takeshita E, Kume A, Maeda Y, Sakai H, Sakane F
Biochemical and biophysical research communications444(4)617-21(2014 Feb)

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Gellett AM, Kharel Y, Sunkara M, Morris AJ, Lynch KR
Biochemical and biophysical research communications422(4)758-63(2012 Jun)

Analyses of shared genetic factors between asthma and obesity in children.
Mel?n E, Himes BE, Brehm JM, Boutaoui N, Klanderman BJ, Sylvia JS, Lasky-Su J
The Journal of allergy and clinical immunology126(3)631-7.e1-8(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Epigenetic silencing of DGKG expression is associated with skin cancer.
Title: Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.

these results indicate that DGKG is an anionic phospholipid binding protein that preferably interacts with a small highly charged head group that is very close to the glycerol or sphingosine backbone.
Title: Diacylglycerol kinase ? is a novel anionic phospholipid binding protein with a selective binding preference.

Observational study of gene-disease association. (HuGE Navigator)
Title: Analyses of shared genetic factors between asthma and obesity in children.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Implication of genetic variants near NEGR1, SEC16B, TMEM18, ETV5/DGKG, GNPDA2, LIN7C/BDNF, MTCH2, BCDIN3D/FAIM2, SH2B1, FTO, MC4R, and KCTD15 with obesity and type 2 diabetes in 7705 Chinese.

The following DGKG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17424	NM_001346.3 Latest version!	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu17413	NM_001080744.2 Latest version!	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu17440	NM_001080745.2 Latest version!	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu17440	NM_001080745.1	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu17424	NM_001346.2	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu17413	NM_001080744.1	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKG ( NM_001346.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337.2 Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17424
Clone ID Related Accession (Same CDS sequence)	NM_001346.2 , NM_001346.3
Accession Version	NM_001346.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	diacylglycerol kinase gamma isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117414.3, BC089411.1, D26135.1, AC112649.9 and BM669549.1. On Jan 29, 2007 this sequence version replaced NM_001346.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.150694.1, SRR1660809.188900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGA ATTCTTCGGA AATTCCACTA TCTGCTCAAC 
CCCAAACAAG TTTTCAACCT GGACAATGGG GGGCCTACTC CAGGGTTGAA CTTTTTCCGT 
GATACTCCAG ACTTCCGTGT TTTGGCCTGT GGTGGAGATG GGACAGTTGG CTGGATTTTG 
GATTGCATTG ATAAGGCCAA CTTTGCAAAG CATCCACCAG TGGCTGTCCT GCCTCTTGGA 
ACAGGAAATG ACCTTGCCCG TTGTCTCCGC TGGGGAGGAG GTTATGAAGG GGGCAGCTTG 
ACAAAAATCC TGAAAGACAT TGAGCAGAGC CCCTTGGTGA TGCTGGACCG CTGGCATCTG 
GAAGTCATCC CCAGAGAGGA AGTGGAAAAC GGGGACCAGG TCCCATACAG CATCATGAAC 
AACTATTTCT CCATTGGTGT GGACGCTTCC ATTGCACACA GATTCCATGT GATGAGAGAG 
AAACATCCTG AAAAATTCAA CAGCAGGATG AAGAACAAGC TGTGGTACTT TGAATTTGGC 
ACCTCGGAGA CTTTTGCAGC GACCTGCAAG AAACTCCACG ACCACATTGA GTTGGAGTGT 
GATGGGGTTG GGGTGGACCT GAGCAACATC TTCCTGGAAG GCATTGCCAT TCTCAACATT 
CCCAGCATGT ACGGAGGCAC CAATCTCTGG GGAGAAAACA AGAAGAACCG GGCTGTGATC 
CGGGAAAGCA GGAAGGGTGT CACTGACCCC AAAGAACTGA AATTCTGCGT TCAAGACCTC 
AGTGACCAGC TCCTTGAAGT GGTGGGGCTA GAAGGAGCCA TGGAGATGGG GCAGATCTAC 
ACCGGCCTGA AGAGTGCAGG CAGGAGGCTG GCCCAGTGCG CCTCTGTCAC CATCAGGACA 
AACAAGCTGC TGCCAATGCA AGTGGATGGA GAACCCTGGA TGCAGCCATG TTGCACGATT 
AAAATTACTC ACAAGAACCA AGCGCCCATG ATGATGGGGC CTCCCCAGAA GAGCAGCTTC 
TTCTCGTTGA GAAGGAAGAG CCGTTCAAAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337.2
CDS	538..2913
Translation	MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPKQVFNLDNGGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_001346.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346.2

ORF Insert Sequence:

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGA ATTCTTCGGA AATTCCACTA TCTGCTCAAC 
CCCAAACAAG TTTTCAACCT GGACAATGGG GGGCCTACTC CAGGGTTGAA CTTTTTCCGT 
GATACTCCAG ACTTCCGTGT TTTGGCCTGT GGTGGAGATG GGACAGTTGG CTGGATTTTG 
GATTGCATTG ATAAGGCCAA CTTTGCAAAG CATCCACCAG TGGCTGTCCT GCCTCTTGGA 
ACAGGAAATG ACCTTGCCCG TTGTCTCCGC TGGGGAGGAG GTTATGAAGG GGGCAGCTTG 
ACAAAAATCC TGAAAGACAT TGAGCAGAGC CCCTTGGTGA TGCTGGACCG CTGGCATCTG 
GAAGTCATCC CCAGAGAGGA AGTGGAAAAC GGGGACCAGG TCCCATACAG CATCATGAAC 
AACTATTTCT CCATTGGTGT GGACGCTTCC ATTGCACACA GATTCCATGT GATGAGAGAG 
AAACATCCTG AAAAATTCAA CAGCAGGATG AAGAACAAGC TGTGGTACTT TGAATTTGGC 
ACCTCGGAGA CTTTTGCAGC GACCTGCAAG AAACTCCACG ACCACATTGA GTTGGAGTGT 
GATGGGGTTG GGGTGGACCT GAGCAACATC TTCCTGGAAG GCATTGCCAT TCTCAACATT 
CCCAGCATGT ACGGAGGCAC CAATCTCTGG GGAGAAAACA AGAAGAACCG GGCTGTGATC 
CGGGAAAGCA GGAAGGGTGT CACTGACCCC AAAGAACTGA AATTCTGCGT TCAAGACCTC 
AGTGACCAGC TCCTTGAAGT GGTGGGGCTA GAAGGAGCCA TGGAGATGGG GCAGATCTAC 
ACCGGCCTGA AGAGTGCAGG CAGGAGGCTG GCCCAGTGCG CCTCTGTCAC CATCAGGACA 
AACAAGCTGC TGCCAATGCA AGTGGATGGA GAACCCTGGA TGCAGCCATG TTGCACGATT 
AAAATTACTC ACAAGAACCA AGCGCCCATG ATGATGGGGC CTCCCCAGAA GAGCAGCTTC 
TTCTCGTTGA GAAGGAAGAG CCGTTCAAAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKG ( NM_001346.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337.2 Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17424
Clone ID Related Accession (Same CDS sequence)	NM_001346.2 , NM_001346.3
Accession Version	NM_001346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase gamma isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117414.3, BC089411.1, D26135.1, AC112649.9 and BM669549.1. On Nov 22, 2018 this sequence version replaced NM_001346.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.150694.1, SRR1660809.188900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265022.8/ ENSP00000265022.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGA ATTCTTCGGA AATTCCACTA TCTGCTCAAC 
CCCAAACAAG TTTTCAACCT GGACAATGGG GGGCCTACTC CAGGGTTGAA CTTTTTCCGT 
GATACTCCAG ACTTCCGTGT TTTGGCCTGT GGTGGAGATG GGACAGTTGG CTGGATTTTG 
GATTGCATTG ATAAGGCCAA CTTTGCAAAG CATCCACCAG TGGCTGTCCT GCCTCTTGGA 
ACAGGAAATG ACCTTGCCCG TTGTCTCCGC TGGGGAGGAG GTTATGAAGG GGGCAGCTTG 
ACAAAAATCC TGAAAGACAT TGAGCAGAGC CCCTTGGTGA TGCTGGACCG CTGGCATCTG 
GAAGTCATCC CCAGAGAGGA AGTGGAAAAC GGGGACCAGG TCCCATACAG CATCATGAAC 
AACTATTTCT CCATTGGTGT GGACGCTTCC ATTGCACACA GATTCCATGT GATGAGAGAG 
AAACATCCTG AAAAATTCAA CAGCAGGATG AAGAACAAGC TGTGGTACTT TGAATTTGGC 
ACCTCGGAGA CTTTTGCAGC GACCTGCAAG AAACTCCACG ACCACATTGA GTTGGAGTGT 
GATGGGGTTG GGGTGGACCT GAGCAACATC TTCCTGGAAG GCATTGCCAT TCTCAACATT 
CCCAGCATGT ACGGAGGCAC CAATCTCTGG GGAGAAAACA AGAAGAACCG GGCTGTGATC 
CGGGAAAGCA GGAAGGGTGT CACTGACCCC AAAGAACTGA AATTCTGCGT TCAAGACCTC 
AGTGACCAGC TCCTTGAAGT GGTGGGGCTA GAAGGAGCCA TGGAGATGGG GCAGATCTAC 
ACCGGCCTGA AGAGTGCAGG CAGGAGGCTG GCCCAGTGCG CCTCTGTCAC CATCAGGACA 
AACAAGCTGC TGCCAATGCA AGTGGATGGA GAACCCTGGA TGCAGCCATG TTGCACGATT 
AAAATTACTC ACAAGAACCA AGCGCCCATG ATGATGGGGC CTCCCCAGAA GAGCAGCTTC 
TTCTCGTTGA GAAGGAAGAG CCGTTCAAAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337.2
CDS	538..2913
Translation	MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPKQVFNLDNGGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_001346.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346.3

ORF Insert Sequence:

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGA ATTCTTCGGA AATTCCACTA TCTGCTCAAC 
CCCAAACAAG TTTTCAACCT GGACAATGGG GGGCCTACTC CAGGGTTGAA CTTTTTCCGT 
GATACTCCAG ACTTCCGTGT TTTGGCCTGT GGTGGAGATG GGACAGTTGG CTGGATTTTG 
GATTGCATTG ATAAGGCCAA CTTTGCAAAG CATCCACCAG TGGCTGTCCT GCCTCTTGGA 
ACAGGAAATG ACCTTGCCCG TTGTCTCCGC TGGGGAGGAG GTTATGAAGG GGGCAGCTTG 
ACAAAAATCC TGAAAGACAT TGAGCAGAGC CCCTTGGTGA TGCTGGACCG CTGGCATCTG 
GAAGTCATCC CCAGAGAGGA AGTGGAAAAC GGGGACCAGG TCCCATACAG CATCATGAAC 
AACTATTTCT CCATTGGTGT GGACGCTTCC ATTGCACACA GATTCCATGT GATGAGAGAG 
AAACATCCTG AAAAATTCAA CAGCAGGATG AAGAACAAGC TGTGGTACTT TGAATTTGGC 
ACCTCGGAGA CTTTTGCAGC GACCTGCAAG AAACTCCACG ACCACATTGA GTTGGAGTGT 
GATGGGGTTG GGGTGGACCT GAGCAACATC TTCCTGGAAG GCATTGCCAT TCTCAACATT 
CCCAGCATGT ACGGAGGCAC CAATCTCTGG GGAGAAAACA AGAAGAACCG GGCTGTGATC 
CGGGAAAGCA GGAAGGGTGT CACTGACCCC AAAGAACTGA AATTCTGCGT TCAAGACCTC 
AGTGACCAGC TCCTTGAAGT GGTGGGGCTA GAAGGAGCCA TGGAGATGGG GCAGATCTAC 
ACCGGCCTGA AGAGTGCAGG CAGGAGGCTG GCCCAGTGCG CCTCTGTCAC CATCAGGACA 
AACAAGCTGC TGCCAATGCA AGTGGATGGA GAACCCTGGA TGCAGCCATG TTGCACGATT 
AAAATTACTC ACAAGAACCA AGCGCCCATG ATGATGGGGC CTCCCCAGAA GAGCAGCTTC 
TTCTCGTTGA GAAGGAAGAG CCGTTCAAAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKG ( NM_001080744.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074213.1 Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17413
Clone ID Related Accession (Same CDS sequence)	NM_001080744.2 , NM_001080744.1
Accession Version	NM_001080744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase gamma isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117414.3, BC089411.1, AC112649.9 and BM669549.1. On May 31, 2019 this sequence version replaced NM_001080744.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGG TTGAACTTTT TCCGTGATAC TCCAGACTTC 
CGTGTTTTGG CCTGTGGTGG AGATGGGACA GTTGGCTGGA TTTTGGATTG CATTGATAAG 
GCCAACTTTG CAAAGCATCC ACCAGTGGCT GTCCTGCCTC TTGGAACAGG AAATGACCTT 
GCCCGTTGTC TCCGCTGGGG AGGAGGTTAT GAAGGGGGCA GCTTGACAAA AATCCTGAAA 
GACATTGAGC AGAGCCCCTT GGTGATGCTG GACCGCTGGC ATCTGGAAGT CATCCCCAGA 
GAGGAAGTGG AAAACGGGGA CCAGGTCCCA TACAGCATCA TGAACAACTA TTTCTCCATT 
GGTGTGGACG CTTCCATTGC ACACAGATTC CATGTGATGA GAGAGAAACA TCCTGAAAAA 
TTCAACAGCA GGATGAAGAA CAAGCTGTGG TACTTTGAAT TTGGCACCTC GGAGACTTTT 
GCAGCGACCT GCAAGAAACT CCACGACCAC ATTGAGTTGG AGTGTGATGG GGTTGGGGTG 
GACCTGAGCA ACATCTTCCT GGAAGGCATT GCCATTCTCA ACATTCCCAG CATGTACGGA 
GGCACCAATC TCTGGGGAGA AAACAAGAAG AACCGGGCTG TGATCCGGGA AAGCAGGAAG 
GGTGTCACTG ACCCCAAAGA ACTGAAATTC TGCGTTCAAG ACCTCAGTGA CCAGCTCCTT 
GAAGTGGTGG GGCTAGAAGG AGCCATGGAG ATGGGGCAGA TCTACACCGG CCTGAAGAGT 
GCAGGCAGGA GGCTGGCCCA GTGCGCCTCT GTCACCATCA GGACAAACAA GCTGCTGCCA 
ATGCAAGTGG ATGGAGAACC CTGGATGCAG CCATGTTGCA CGATTAAAAT TACTCACAAG 
AACCAAGCGC CCATGATGAT GGGGCCTCCC CAGAAGAGCA GCTTCTTCTC GTTGAGAAGG 
AAGAGCCGTT CAAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074213.1
CDS	538..2838
Translation	MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_001080744.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080744.2

ORF Insert Sequence:

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGG TTGAACTTTT TCCGTGATAC TCCAGACTTC 
CGTGTTTTGG CCTGTGGTGG AGATGGGACA GTTGGCTGGA TTTTGGATTG CATTGATAAG 
GCCAACTTTG CAAAGCATCC ACCAGTGGCT GTCCTGCCTC TTGGAACAGG AAATGACCTT 
GCCCGTTGTC TCCGCTGGGG AGGAGGTTAT GAAGGGGGCA GCTTGACAAA AATCCTGAAA 
GACATTGAGC AGAGCCCCTT GGTGATGCTG GACCGCTGGC ATCTGGAAGT CATCCCCAGA 
GAGGAAGTGG AAAACGGGGA CCAGGTCCCA TACAGCATCA TGAACAACTA TTTCTCCATT 
GGTGTGGACG CTTCCATTGC ACACAGATTC CATGTGATGA GAGAGAAACA TCCTGAAAAA 
TTCAACAGCA GGATGAAGAA CAAGCTGTGG TACTTTGAAT TTGGCACCTC GGAGACTTTT 
GCAGCGACCT GCAAGAAACT CCACGACCAC ATTGAGTTGG AGTGTGATGG GGTTGGGGTG 
GACCTGAGCA ACATCTTCCT GGAAGGCATT GCCATTCTCA ACATTCCCAG CATGTACGGA 
GGCACCAATC TCTGGGGAGA AAACAAGAAG AACCGGGCTG TGATCCGGGA AAGCAGGAAG 
GGTGTCACTG ACCCCAAAGA ACTGAAATTC TGCGTTCAAG ACCTCAGTGA CCAGCTCCTT 
GAAGTGGTGG GGCTAGAAGG AGCCATGGAG ATGGGGCAGA TCTACACCGG CCTGAAGAGT 
GCAGGCAGGA GGCTGGCCCA GTGCGCCTCT GTCACCATCA GGACAAACAA GCTGCTGCCA 
ATGCAAGTGG ATGGAGAACC CTGGATGCAG CCATGTTGCA CGATTAAAAT TACTCACAAG 
AACCAAGCGC CCATGATGAT GGGGCCTCCC CAGAAGAGCA GCTTCTTCTC GTTGAGAAGG 
AAGAGCCGTT CAAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKG ( NM_001080744.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074213.1 Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17413
Clone ID Related Accession (Same CDS sequence)	NM_001080744.2 , NM_001080744.1
Accession Version	NM_001080744.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	diacylglycerol kinase gamma isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117414.3, BC089411.1, AC112649.9 and BM669549.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGG TTGAACTTTT TCCGTGATAC TCCAGACTTC 
CGTGTTTTGG CCTGTGGTGG AGATGGGACA GTTGGCTGGA TTTTGGATTG CATTGATAAG 
GCCAACTTTG CAAAGCATCC ACCAGTGGCT GTCCTGCCTC TTGGAACAGG AAATGACCTT 
GCCCGTTGTC TCCGCTGGGG AGGAGGTTAT GAAGGGGGCA GCTTGACAAA AATCCTGAAA 
GACATTGAGC AGAGCCCCTT GGTGATGCTG GACCGCTGGC ATCTGGAAGT CATCCCCAGA 
GAGGAAGTGG AAAACGGGGA CCAGGTCCCA TACAGCATCA TGAACAACTA TTTCTCCATT 
GGTGTGGACG CTTCCATTGC ACACAGATTC CATGTGATGA GAGAGAAACA TCCTGAAAAA 
TTCAACAGCA GGATGAAGAA CAAGCTGTGG TACTTTGAAT TTGGCACCTC GGAGACTTTT 
GCAGCGACCT GCAAGAAACT CCACGACCAC ATTGAGTTGG AGTGTGATGG GGTTGGGGTG 
GACCTGAGCA ACATCTTCCT GGAAGGCATT GCCATTCTCA ACATTCCCAG CATGTACGGA 
GGCACCAATC TCTGGGGAGA AAACAAGAAG AACCGGGCTG TGATCCGGGA AAGCAGGAAG 
GGTGTCACTG ACCCCAAAGA ACTGAAATTC TGCGTTCAAG ACCTCAGTGA CCAGCTCCTT 
GAAGTGGTGG GGCTAGAAGG AGCCATGGAG ATGGGGCAGA TCTACACCGG CCTGAAGAGT 
GCAGGCAGGA GGCTGGCCCA GTGCGCCTCT GTCACCATCA GGACAAACAA GCTGCTGCCA 
ATGCAAGTGG ATGGAGAACC CTGGATGCAG CCATGTTGCA CGATTAAAAT TACTCACAAG 
AACCAAGCGC CCATGATGAT GGGGCCTCCC CAGAAGAGCA GCTTCTTCTC GTTGAGAAGG 
AAGAGCCGTT CAAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074213.1
CDS	538..2838
Translation	MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_001080744.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080744.1

ORF Insert Sequence:

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGG TGATGCAGCA CGCATGGGTG 
GAAGGGAACT CCTCCGTCAA GTGTGACCGG TGCCACAAAA GTATCAAGTG CTACCAGAGT 
GTCACCGCGC GGCACTGCGT GTGGTGCCGG ATGACGTTTC ACCGCAAATG TGAATTATCA 
ACGTTGTGTG ACGGTGGGGA ACTCAGAGAC CACATCTTAC TGCCCACCTC CATATGCCCC 
ATCACCCGGG ACAGGCCAGG TGAGAAGTCT GATGGCTGCG TGTCCGCCAA GGGCGAACTT 
GTCATGCAGT ATAAGATCAT CCCCACCCCG GGTACCCACC CCCTGCTGGT CTTGGTGAAC 
CCCAAGAGTG GAGGGAGACA AGGAGAAAGG TTGAACTTTT TCCGTGATAC TCCAGACTTC 
CGTGTTTTGG CCTGTGGTGG AGATGGGACA GTTGGCTGGA TTTTGGATTG CATTGATAAG 
GCCAACTTTG CAAAGCATCC ACCAGTGGCT GTCCTGCCTC TTGGAACAGG AAATGACCTT 
GCCCGTTGTC TCCGCTGGGG AGGAGGTTAT GAAGGGGGCA GCTTGACAAA AATCCTGAAA 
GACATTGAGC AGAGCCCCTT GGTGATGCTG GACCGCTGGC ATCTGGAAGT CATCCCCAGA 
GAGGAAGTGG AAAACGGGGA CCAGGTCCCA TACAGCATCA TGAACAACTA TTTCTCCATT 
GGTGTGGACG CTTCCATTGC ACACAGATTC CATGTGATGA GAGAGAAACA TCCTGAAAAA 
TTCAACAGCA GGATGAAGAA CAAGCTGTGG TACTTTGAAT TTGGCACCTC GGAGACTTTT 
GCAGCGACCT GCAAGAAACT CCACGACCAC ATTGAGTTGG AGTGTGATGG GGTTGGGGTG 
GACCTGAGCA ACATCTTCCT GGAAGGCATT GCCATTCTCA ACATTCCCAG CATGTACGGA 
GGCACCAATC TCTGGGGAGA AAACAAGAAG AACCGGGCTG TGATCCGGGA AAGCAGGAAG 
GGTGTCACTG ACCCCAAAGA ACTGAAATTC TGCGTTCAAG ACCTCAGTGA CCAGCTCCTT 
GAAGTGGTGG GGCTAGAAGG AGCCATGGAG ATGGGGCAGA TCTACACCGG CCTGAAGAGT 
GCAGGCAGGA GGCTGGCCCA GTGCGCCTCT GTCACCATCA GGACAAACAA GCTGCTGCCA 
ATGCAAGTGG ATGGAGAACC CTGGATGCAG CCATGTTGCA CGATTAAAAT TACTCACAAG 
AACCAAGCGC CCATGATGAT GGGGCCTCCC CAGAAGAGCA GCTTCTTCTC GTTGAGAAGG 
AAGAGCCGTT CAAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKG ( NM_001080745.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074214.1 Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu17440
Clone ID Related Accession (Same CDS sequence)	NM_001080745.2 , NM_001080745.1
Accession Version	NM_001080745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase gamma isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117414.3, BC089411.1, BC112363.1, AC112649.9 and BM669549.1. On Jun 1, 2019 this sequence version replaced NM_001080745.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 3) that is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.224394.1, SRR1660807.231651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGT TTCACCGCAA ATGTGAATTA 
TCAACGTTGT GTGACGGTGG GGAACTCAGA GACCACATCT TACTGCCCAC CTCCATATGC 
CCCATCACCC GGGACAGGCC AGGTGAGAAG TCTGATGGCT GCGTGTCCGC CAAGGGCGAA 
CTTGTCATGC AGTATAAGAT CATCCCCACC CCGGGTACCC ACCCCCTGCT GGTCTTGGTG 
AACCCCAAGA GTGGAGGGAG ACAAGGAGAA AGAATTCTTC GGAAATTCCA CTATCTGCTC 
AACCCCAAAC AAGTTTTCAA CCTGGACAAT GGGGGGCCTA CTCCAGGGTT GAACTTTTTC 
CGTGATACTC CAGACTTCCG TGTTTTGGCC TGTGGTGGAG ATGGGACAGT TGGCTGGATT 
TTGGATTGCA TTGATAAGGC CAACTTTGCA AAGCATCCAC CAGTGGCTGT CCTGCCTCTT 
GGAACAGGAA ATGACCTTGC CCGTTGTCTC CGCTGGGGAG GAGGTTATGA AGGGGGCAGC 
TTGACAAAAA TCCTGAAAGA CATTGAGCAG AGCCCCTTGG TGATGCTGGA CCGCTGGCAT 
CTGGAAGTCA TCCCCAGAGA GGAAGTGGAA AACGGGGACC AGGTCCCATA CAGCATCATG 
AACAACTATT TCTCCATTGG TGTGGACGCT TCCATTGCAC ACAGATTCCA TGTGATGAGA 
GAGAAACATC CTGAAAAATT CAACAGCAGG ATGAAGAACA AGCTGTGGTA CTTTGAATTT 
GGCACCTCGG AGACTTTTGC AGCGACCTGC AAGAAACTCC ACGACCACAT TGAGTTGGAG 
TGTGATGGGG TTGGGGTGGA CCTGAGCAAC ATCTTCCTGG AAGGCATTGC CATTCTCAAC 
ATTCCCAGCA TGTACGGAGG CACCAATCTC TGGGGAGAAA ACAAGAAGAA CCGGGCTGTG 
ATCCGGGAAA GCAGGAAGGG TGTCACTGAC CCCAAAGAAC TGAAATTCTG CGTTCAAGAC 
CTCAGTGACC AGCTCCTTGA AGTGGTGGGG CTAGAAGGAG CCATGGAGAT GGGGCAGATC 
TACACCGGCC TGAAGAGTGC AGGCAGGAGG CTGGCCCAGT GCGCCTCTGT CACCATCAGG 
ACAAACAAGC TGCTGCCAAT GCAAGTGGAT GGAGAACCCT GGATGCAGCC ATGTTGCACG 
ATTAAAATTA CTCACAAGAA CCAAGCGCCC ATGATGATGG GGCCTCCCCA GAAGAGCAGC 
TTCTTCTCGT TGAGAAGGAA GAGCCGTTCA AAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074214.1
CDS	538..2796
Translation	MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPKQVFNLDNGGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_001080745.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080745.2

ORF Insert Sequence:

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGT TTCACCGCAA ATGTGAATTA 
TCAACGTTGT GTGACGGTGG GGAACTCAGA GACCACATCT TACTGCCCAC CTCCATATGC 
CCCATCACCC GGGACAGGCC AGGTGAGAAG TCTGATGGCT GCGTGTCCGC CAAGGGCGAA 
CTTGTCATGC AGTATAAGAT CATCCCCACC CCGGGTACCC ACCCCCTGCT GGTCTTGGTG 
AACCCCAAGA GTGGAGGGAG ACAAGGAGAA AGAATTCTTC GGAAATTCCA CTATCTGCTC 
AACCCCAAAC AAGTTTTCAA CCTGGACAAT GGGGGGCCTA CTCCAGGGTT GAACTTTTTC 
CGTGATACTC CAGACTTCCG TGTTTTGGCC TGTGGTGGAG ATGGGACAGT TGGCTGGATT 
TTGGATTGCA TTGATAAGGC CAACTTTGCA AAGCATCCAC CAGTGGCTGT CCTGCCTCTT 
GGAACAGGAA ATGACCTTGC CCGTTGTCTC CGCTGGGGAG GAGGTTATGA AGGGGGCAGC 
TTGACAAAAA TCCTGAAAGA CATTGAGCAG AGCCCCTTGG TGATGCTGGA CCGCTGGCAT 
CTGGAAGTCA TCCCCAGAGA GGAAGTGGAA AACGGGGACC AGGTCCCATA CAGCATCATG 
AACAACTATT TCTCCATTGG TGTGGACGCT TCCATTGCAC ACAGATTCCA TGTGATGAGA 
GAGAAACATC CTGAAAAATT CAACAGCAGG ATGAAGAACA AGCTGTGGTA CTTTGAATTT 
GGCACCTCGG AGACTTTTGC AGCGACCTGC AAGAAACTCC ACGACCACAT TGAGTTGGAG 
TGTGATGGGG TTGGGGTGGA CCTGAGCAAC ATCTTCCTGG AAGGCATTGC CATTCTCAAC 
ATTCCCAGCA TGTACGGAGG CACCAATCTC TGGGGAGAAA ACAAGAAGAA CCGGGCTGTG 
ATCCGGGAAA GCAGGAAGGG TGTCACTGAC CCCAAAGAAC TGAAATTCTG CGTTCAAGAC 
CTCAGTGACC AGCTCCTTGA AGTGGTGGGG CTAGAAGGAG CCATGGAGAT GGGGCAGATC 
TACACCGGCC TGAAGAGTGC AGGCAGGAGG CTGGCCCAGT GCGCCTCTGT CACCATCAGG 
ACAAACAAGC TGCTGCCAAT GCAAGTGGAT GGAGAACCCT GGATGCAGCC ATGTTGCACG 
ATTAAAATTA CTCACAAGAA CCAAGCGCCC ATGATGATGG GGCCTCCCCA GAAGAGCAGC 
TTCTTCTCGT TGAGAAGGAA GAGCCGTTCA AAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKG ( NM_001080745.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074214.1 Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu17440
Clone ID Related Accession (Same CDS sequence)	NM_001080745.2 , NM_001080745.1
Accession Version	NM_001080745.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	diacylglycerol kinase gamma isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117414.3, BC089411.1, BC112363.1, AC112649.9 and BM669549.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 3) that is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.20834.1, SRR1660809.5098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGT TTCACCGCAA ATGTGAATTA 
TCAACGTTGT GTGACGGTGG GGAACTCAGA GACCACATCT TACTGCCCAC CTCCATATGC 
CCCATCACCC GGGACAGGCC AGGTGAGAAG TCTGATGGCT GCGTGTCCGC CAAGGGCGAA 
CTTGTCATGC AGTATAAGAT CATCCCCACC CCGGGTACCC ACCCCCTGCT GGTCTTGGTG 
AACCCCAAGA GTGGAGGGAG ACAAGGAGAA AGAATTCTTC GGAAATTCCA CTATCTGCTC 
AACCCCAAAC AAGTTTTCAA CCTGGACAAT GGGGGGCCTA CTCCAGGGTT GAACTTTTTC 
CGTGATACTC CAGACTTCCG TGTTTTGGCC TGTGGTGGAG ATGGGACAGT TGGCTGGATT 
TTGGATTGCA TTGATAAGGC CAACTTTGCA AAGCATCCAC CAGTGGCTGT CCTGCCTCTT 
GGAACAGGAA ATGACCTTGC CCGTTGTCTC CGCTGGGGAG GAGGTTATGA AGGGGGCAGC 
TTGACAAAAA TCCTGAAAGA CATTGAGCAG AGCCCCTTGG TGATGCTGGA CCGCTGGCAT 
CTGGAAGTCA TCCCCAGAGA GGAAGTGGAA AACGGGGACC AGGTCCCATA CAGCATCATG 
AACAACTATT TCTCCATTGG TGTGGACGCT TCCATTGCAC ACAGATTCCA TGTGATGAGA 
GAGAAACATC CTGAAAAATT CAACAGCAGG ATGAAGAACA AGCTGTGGTA CTTTGAATTT 
GGCACCTCGG AGACTTTTGC AGCGACCTGC AAGAAACTCC ACGACCACAT TGAGTTGGAG 
TGTGATGGGG TTGGGGTGGA CCTGAGCAAC ATCTTCCTGG AAGGCATTGC CATTCTCAAC 
ATTCCCAGCA TGTACGGAGG CACCAATCTC TGGGGAGAAA ACAAGAAGAA CCGGGCTGTG 
ATCCGGGAAA GCAGGAAGGG TGTCACTGAC CCCAAAGAAC TGAAATTCTG CGTTCAAGAC 
CTCAGTGACC AGCTCCTTGA AGTGGTGGGG CTAGAAGGAG CCATGGAGAT GGGGCAGATC 
TACACCGGCC TGAAGAGTGC AGGCAGGAGG CTGGCCCAGT GCGCCTCTGT CACCATCAGG 
ACAAACAAGC TGCTGCCAAT GCAAGTGGAT GGAGAACCCT GGATGCAGCC ATGTTGCACG 
ATTAAAATTA CTCACAAGAA CCAAGCGCCC ATGATGATGG GGCCTCCCCA GAAGAGCAGC 
TTCTTCTCGT TGAGAAGGAA GAGCCGTTCA AAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074214.1
CDS	538..2796
Translation	MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPLLVLLGMDDSGSKGDGRHAWTMKHFKKPTYCNFCHIMLMGVRKQGLCCTYCKYTVHERCVSRNIPGCVKTYSKAKRSGEFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGELVMQYKIIPTPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPKQVFNLDNGGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_001080745.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase gamma (DGKG), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080745.1

ORF Insert Sequence:

ATGGGTGAAG AACGGTGGGT CTCCCTCACT CCAGAAGAAT TTGACCAACT CCAGAAATAT 
TCAGAATATT CCTCCAAGAA GATAAAAGAT GCCTTGACTG AATTTAATGA GGGTGGGAGC 
CTCAAACAAT ATGACCCACA TGAGCCGATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCGTACCTGG AGGTGGACCT TCCCCAGCCA CTGAGCACTC ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACACGAGAC CTCTGACCAC CCGACGGAGG GAGCCAGCAA CAGTGAGGCC 
AACAGCGCAG ATACTAATAT ACAGAATGCA GATAATGCCA CCAAAGCAGA CGAGGCCTGT 
GCCCCTGATA CTGAATCAAA TATGGCTGAG AAGCAAGCAC CAGCTGAAGA CCAAGTGGCT 
GCGACCCCCC TGGAACCCCC CGTCCCTCGG TCTTCAAGCT CGGAATCCCC AGTGGTATAC 
CTGAAGGATG TTGTGTGCTA CCTGTCCCTG CTGGAGACGG GGAGGCCTCA GGATAAGCTG 
GAGTTCATGT TTCGCCTCTA TGATTCAGAT GAGAACGGTC TCCTGGACCA AGCGGAGATG 
GATTGCATTG TCAACCAAAT GCTGCATATT GCCCAGTACC TGGAGTGGGA TCCCACAGAG 
CTGAGGCCTA TATTGAAGGA GATGCTGCAA GGGATGGACT ACGACCGGGA CGGCTTTGTG 
TCTCTACAGG AATGGGTCCA TGGAGGGATG ACCACCATCC CATTGCTGGT CCTCCTGGGG 
ATGGATGACT CTGGCTCCAA GGGGGATGGG CGGCACGCCT GGACCATGAA GCACTTCAAG 
AAACCAACCT ACTGCAACTT CTGCCATATC ATGCTCATGG GCGTCCGCAA GCAAGGCCTG 
TGCTGCACTT ACTGTAAATA CACTGTCCAC GAACGCTGTG TGTCCAGAAA CATTCCTGGT 
TGTGTCAAAA CGTACTCAAA AGCCAAAAGG AGTGGTGAGT TTCACCGCAA ATGTGAATTA 
TCAACGTTGT GTGACGGTGG GGAACTCAGA GACCACATCT TACTGCCCAC CTCCATATGC 
CCCATCACCC GGGACAGGCC AGGTGAGAAG TCTGATGGCT GCGTGTCCGC CAAGGGCGAA 
CTTGTCATGC AGTATAAGAT CATCCCCACC CCGGGTACCC ACCCCCTGCT GGTCTTGGTG 
AACCCCAAGA GTGGAGGGAG ACAAGGAGAA AGAATTCTTC GGAAATTCCA CTATCTGCTC 
AACCCCAAAC AAGTTTTCAA CCTGGACAAT GGGGGGCCTA CTCCAGGGTT GAACTTTTTC 
CGTGATACTC CAGACTTCCG TGTTTTGGCC TGTGGTGGAG ATGGGACAGT TGGCTGGATT 
TTGGATTGCA TTGATAAGGC CAACTTTGCA AAGCATCCAC CAGTGGCTGT CCTGCCTCTT 
GGAACAGGAA ATGACCTTGC CCGTTGTCTC CGCTGGGGAG GAGGTTATGA AGGGGGCAGC 
TTGACAAAAA TCCTGAAAGA CATTGAGCAG AGCCCCTTGG TGATGCTGGA CCGCTGGCAT 
CTGGAAGTCA TCCCCAGAGA GGAAGTGGAA AACGGGGACC AGGTCCCATA CAGCATCATG 
AACAACTATT TCTCCATTGG TGTGGACGCT TCCATTGCAC ACAGATTCCA TGTGATGAGA 
GAGAAACATC CTGAAAAATT CAACAGCAGG ATGAAGAACA AGCTGTGGTA CTTTGAATTT 
GGCACCTCGG AGACTTTTGC AGCGACCTGC AAGAAACTCC ACGACCACAT TGAGTTGGAG 
TGTGATGGGG TTGGGGTGGA CCTGAGCAAC ATCTTCCTGG AAGGCATTGC CATTCTCAAC 
ATTCCCAGCA TGTACGGAGG CACCAATCTC TGGGGAGAAA ACAAGAAGAA CCGGGCTGTG 
ATCCGGGAAA GCAGGAAGGG TGTCACTGAC CCCAAAGAAC TGAAATTCTG CGTTCAAGAC 
CTCAGTGACC AGCTCCTTGA AGTGGTGGGG CTAGAAGGAG CCATGGAGAT GGGGCAGATC 
TACACCGGCC TGAAGAGTGC AGGCAGGAGG CTGGCCCAGT GCGCCTCTGT CACCATCAGG 
ACAAACAAGC TGCTGCCAAT GCAAGTGGAT GGAGAACCCT GGATGCAGCC ATGTTGCACG 
ATTAAAATTA CTCACAAGAA CCAAGCGCCC ATGATGATGG GGCCTCCCCA GAAGAGCAGC 
TTCTTCTCGT TGAGAAGGAA GAGCCGTTCA AAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.
Molecular carcinogenesis56(7)1743-1752(2017 Jul)
Kai M,Yamamoto E,Sato A,Yamano HO,Niinuma T,Kitajima H,Harada T,Aoki H,Maruyama R,Toyota M,Hatahira T,Nakase H,Sugai T,Yamashita T,Toyota M,Suzuki H

Diacylglycerol kinase ? is a novel anionic phospholipid binding protein with a selective binding preference.
Biochemical and biophysical research communications444(4)617-21(2014 Feb)
Takeshita E,Kume A,Maeda Y,Sakai H,Sakane F

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Biochemical and biophysical research communications422(4)758-63(2012 Jun)
Gellett AM,Kharel Y,Sunkara M,Morris AJ,Lynch KR

Analyses of shared genetic factors between asthma and obesity in children.
The Journal of allergy and clinical immunology126(3)631-7.e1-8(2010 Sep)
Mel?n E,Himes BE,Brehm JM,Boutaoui N,Klanderman BJ,Sylvia JS,Lasky-Su J

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DGKG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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