DAG1 cDNA ORF clone, Homo sapiens(human)

The following DAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20436 NM_001177637.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177644.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177638.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_004393.5
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177636.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001165928.3
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177640.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177641.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177639.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177642.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177635.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177643.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20436 NM_001177634.2
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu108851 NM_004393.6
Latest version!
Homo sapiens dystroglycan 1 (DAG1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177643.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU140753.1, AC104452.2, DB089586.1, BC012740.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177643.1. Transcript Variant: This variant (12) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171114.1
    CDS470..3157
    Translation

    Target ORF information:

    RefSeq Version NM_001177643.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177643.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104452.2, DA338757.1, BC012740.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177640.1. Transcript Variant: This variant (9) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.317960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171111.1
    CDS476..3163
    Translation

    Target ORF information:

    RefSeq Version NM_001177640.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177638.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA451012.1, DA843040.1, DB214431.1, BC012740.2, AC104452.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177638.1. Transcript Variant: This variant (7) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171109.1
    CDS570..3257
    Translation

    Target ORF information:

    RefSeq Version NM_001177638.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177638.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177636.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA451012.1, DA843040.1, CN282379.1, AC104452.2, BC012740.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177636.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.149194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171107.1
    CDS701..3388
    Translation

    Target ORF information:

    RefSeq Version NM_001177636.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177636.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177639.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA851197.1, AC104452.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177639.1. Transcript Variant: This variant (8) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA851197.1, DA337917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171110.1
    CDS260..2947
    Translation

    Target ORF information:

    RefSeq Version NM_001177639.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177639.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177641.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA451012.1, DA843040.1, DA698126.1, BC012740.2, AC104452.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177641.1. Transcript Variant: This variant (10) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171112.1
    CDS566..3253
    Translation

    Target ORF information:

    RefSeq Version NM_001177641.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177641.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177637.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104452.2, BP200440.1, BC012740.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177637.1. Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMN03267755 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171108.1
    CDS539..3226
    Translation

    Target ORF information:

    RefSeq Version NM_001177637.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177637.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001165928.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Valerie Delague, Johan den Dunnen, Isabelle Richard, James Ware. The reference sequence was derived from DA451012.1, DA843040.1, DB065493.1, AC104452.2, BC012740.2 and BM990874.1. This sequence is a reference standard in the RefSeqGene project. On Nov 30, 2011 this sequence version replaced NM_001165928.2. Transcript Variant: This variant (1) represents the longest transcript. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001159400.2
    CDS780..3467
    Translation

    Target ORF information:

    RefSeq Version NM_001165928.3
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001165928.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177642.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104452.2, DB087722.1, BC012740.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177642.1. Transcript Variant: This variant (11) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171113.1
    CDS471..3158
    Translation

    Target ORF information:

    RefSeq Version NM_001177642.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177642.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_004393.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-07
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA451012.1, L19711.1, AC104452.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_004393.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L19711.1, BC012740.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004384.4
    CDS419..3106
    Translation

    Target ORF information:

    RefSeq Version NM_004393.5
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004393.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177634.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA451012.1, DA843040.1, BX384229.2, BC012740.2, AC104452.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177634.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX384229.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171105.1
    CDS723..3410
    Translation

    Target ORF information:

    RefSeq Version NM_001177634.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177634.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177644.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104452.2, DB214875.1, BC012740.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177644.1. Transcript Variant: This variant (13) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L19711.1, SRR1660803.75512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171115.1
    CDS559..3246
    Translation

    Target ORF information:

    RefSeq Version NM_001177644.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177644.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20436
    Clone ID Related Accession (Same CDS sequence) NM_001177643.2 , NM_001177640.2 , NM_001177638.2 , NM_001177636.2 , NM_001177639.2 , NM_001177641.2 , NM_001177637.2 , NM_001165928.3 , NM_001177642.2 , NM_004393.5 , NM_001177634.2 , NM_001177644.2 , NM_001177635.2
    Accession Version NM_001177635.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA451012.1, DA843040.1, DA223570.1, AC104452.2 and BM990874.1. On Nov 30, 2011 this sequence version replaced NM_001177635.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA223570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171106.1
    CDS312..2999
    Translation

    Target ORF information:

    RefSeq Version NM_001177635.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177635.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT GGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108851
    Clone ID Related Accession (Same CDS sequence) NM_004393.6
    Accession Version NM_004393.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product dystroglycan preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L19711.1, AC104452.2 and BM990874.1. On Jul 15, 2019 this sequence version replaced NM_004393.5. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2732641.1, SRR7346977.2816274.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT CGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004384.5
    CDS284..2971
    Translation

    Target ORF information:

    RefSeq Version NM_004393.6
    Organism Homo sapiens(human)
    Definition Homo sapiens dystroglycan 1 (DAG1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004393.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGGATGT CTGTGGGCCT CTCGCTGCTG CTGCCCCTCT CGGGGAGGAC CTTTCTCCTC 
    CTGCTCTCTG TGGTTATGGC TCAGTCCCAC TGGCCCAGTG AACCCTCAGA GGCTGTCAGG
    GACTGGGAAA ACCAGCTTGA GGCATCCATG CACTCAGTGC TCTCAGACCT CCACGAGGCT
    GTTCCCACAG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGGCGCTC ATTTCGAGTG
    ACCATTCCAA CAGATTTGAT TGCCTCCAGT GGAGATATCA TCAAGGTATC AGCGGCAGGG
    AAGGAGGCTT TGCCATCTTG GCTGCACTGG GACTCACAGA GCCACACCCT GGAGGGCCTC
    CCCCTTGACA CTGATAAGGG TGTGCATTAC ATTTCAGTGA GCGCTACACG GCTGGGGGCC
    AACGGGAGCC ACATCCCCCA GACCTCCAGT GTGTTCTCCA TCGAGGTCTA CCCTGAAGAC
    CACAGTGAGC TGCAGTCGGT GAGGACAGCC TCCCCAGACC CTGGTGAGGT GGTATCATCT
    GCCTGTGCTG CGGATGAACC TGTGACTGTT TTGACGGTGA TTTTGGATGC CGACCTCACC
    AAGATGACCC CAAAGCAAAG GATTGACCTC CTGCACAGGA TGCGGAGCTT CTCAGAAGTA
    GAGCTTCACA ACATGAAATT AGTGCCGGTG GTGAATAACA GACTATTTGA CATGTCGGCC
    TTCATGGCTG GCCCGGGAAA TGCAAAAAAG GTGGTGGAGA ATGGGGCCCT TCTCTCCTGG
    AAGCTGGGCT GCTCCCTGAA CCAGAACAGT GTGCCTGACA TTCATGGTGT AGAGGCCCCT
    GCCAGGGAGG GCGCAATGTC TGCTCAGCTT GGCTACCCTG TGGTGGGTTG GCACATCGCC
    AATAAGAAGC CCCCTCTTCC CAAACGCGTC CGGAGGCAGA TCCATGCTAC ACCCACACCT
    GTCACTGCCA TTGGGCCCCC AACCACGGCT ATCCAGGAGC CCCCATCCAG GATCGTGCCA
    ACCCCCACAT CTCCAGCCAT TGCTCCTCCA ACAGAGACCA TGGCTCCTCC AGTCAGGGAT
    CCTGTTCCTG GGAAACCCAC GGTCACCATC CGGACTCGAG GCGCCATTAT TCAAACCCCA
    ACCCTAGGCC CCATCCAGCC TACTCGGGTG TCAGAAGCTG GCACCACAGT TCCTGGCCAG
    ATTCGCCCAA CGATGACCAT TCCTGGCTAT GTGGAGCCTA CTGCAGTTGC TACCCCTCCC
    ACAACCACCA CCAAGAAGCC ACGAGTATCC ACACCAAAAC CAGCAACGCC TTCAACTGAC
    TCCACCACCA CCACGACTCG CAGGCCAACC AAGAAACCAC GGACACCCCG GCCAGTGCCC
    CGGGTCACCA CCAAAGTTTC CATCACCAGA TTGGAAACTG CCTCACCGCC TACTCGTATT
    CGCACCACCA CCAGTGGAGT GCCCCGTGGC GGAGAACCCA ACCAGCGCCC AGAGCTCAAG
    AACCATATTG ACAGGGTAGA TGCCTGGGTT GGCACCTACT TTGAGGTGAA GATCCCGTCA
    GACACTTTCT ATGACCATGA GGACACCACC ACTGACAAGC TGAAGCTGAC CCTGAAACTG
    CGGGAGCAGC AGCTGGTGGG CGAGAAGTCC TGGGTACAGT TCAACAGCAA CAGCCAGCTC
    ATGTATGGCC TTCCCGACAG CAGCCACGTG GGCAAACACG AGTATTTCAT GCATGCCACA
    GACAAGGGGG GCCTGTCGGC TGTGGATGCC TTCGAGATCC ACGTCCACAG GCGCCCCCAA
    GGGGATAGGG CTCCTGCAAG GTTCAAGGCC AAGTTTGTGG GTGACCCGGC ACTGGTGTTG
    AATGACATCC ACAAGAAGAT TGCCTTGGTA AAGAAACTGG CCTTCGCCTT TGGAGACCGA
    AACTGTAGCA CCATCACCCT GCAGAATATC ACCCGGGGCT CCATCGTGGT GGAATGGACC
    AACAACACAC TGCCCTTGGA GCCCTGCCCC AAGGAGCAGA TCGCTGGGCT GAGCCGCCGG
    ATCGCTGAGG ATGATGGAAA ACCTCGGCCT GCCTTCTCCA ACGCCCTAGA GCCTGACTTT
    AAGGCCACAA GCATCACTGT GACGGGCTCT GGCAGTTGTC GGCACCTACA GTTTATCCCT
    GTGGTACCAC CCAGGAGAGT GCCCTCAGAG GCGCCGCCCA CAGAAGTGCC TGACAGGGAC
    CCTGAGAAGA GCAGTGAGGA TGATGTCTAC CTGCACACAG TCATTCCGGC CGTGGTGGTC
    GCAGCCATCC TGCTCATTGC TGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG
    GGCAAGCTTA CCCTTGAGGA CCAGGCCACC TTCATCAAGA AGGGGGTGCC TATCATCTTT
    GCAGACGAAC TGGACGACTC CAAGCCCCCA CCCTCCTCCA GCATGCCACT CATTCTGCAG
    GAGGAGAAGG CTCCCCTACC CCCTCCTGAG TACCCCAACC AGAGTGTGCC CGAGACCACT
    CCTCTGAACC AGGACACCAT GGGAGAGTAC ACGCCCCTGC GGGATGAGGA TCCCAATGCG
    CCTCCCTACC AGCCCCCACC GCCCTTCACA GCACCCATGG AGGGCAAGGG CTCCCGTCCC
    AAGAACATGA CCCCATACCG GTCACCTCCT CCCTATGTCC CACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.