DAD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAD1
Entrez Gene ID	1603
Full Name	defender against cell death 1
Synonyms	OST2
General protein information	Preferred Names defender against cell death 1 Names dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 DAD-1 oligosaccharyl transferase subunit DAD1 oligosaccharyltransferase 2 homolog oligosaccharyltransferase subunit 2 (non-catalytic)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2
Summary	DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008].
Disorder MIM:	600243

mRNA and Protein(s)

mRNA	Protein	Name
NM_001344.3	NP_001335.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
NM_001344.4	NP_001335.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_05758	XP_003710680.1
Arabidopsis thaliana (thale cress)	ATDAD1	NP_174500.1
Arabidopsis thaliana (thale cress)	DAD2	NP_850247.1
Homo sapiens (human)	DAD1	NP_001335.1
Pan troglodytes (chimpanzee)	DAD1	XP_509838.2
Oryza sativa (rice)	Os04g0397000	NP_001052664.1
Canis lupus familiaris (dog)	DAD1	XP_537361.2
Danio rerio (zebrafish)	dad1	NP_001093909.1
Drosophila melanogaster (fruit fly)	l(2)k12914	NP_609222.1
Xenopus tropicalis (tropical clawed frog)	dad1	NP_001016940.1
	DAD1	XP_001099561.1
Bos taurus (cattle)	DAD1	NP_001029933.1
Mus musculus (house mouse)	Dad1	NP_034145.1
Rattus norvegicus (Norway rat)	Dad1	NP_620265.1
Gallus gallus (chicken)	DAD1	NP_001007474.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008491	XP_316953.2
Caenorhabditis elegans (roundworm)	dad-1	NP_491889.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative proteomic analysis of CD34(+) cells in bone marrow between severe aplastic anemia and normal control.
Qi W, Fu R, Wang H, Liu C, Ren Y, Shao Y, Shao Z
Cellular immunology304-3059-15(2016 Jun-Jul)

Common variants in left/right asymmetry genes and pathways are associated with relative hand skill.
Brandler WM, Morris AP, Evans DM, Scerri TS, Kemp JP, Timpson NJ, St Pourcain B, Smith GD, Ring SM, Stein J, Monaco AP, Talcott JB, Fisher SE, Webber C, Paracchini S
PLoS genetics9(9)e1003751(2013)

Genetic associations with sporadic neuroendocrine tumor risk.
Ter-Minassian M, Wang Z, Asomaning K, Wu MC, Liu CY, Paulus JK, Liu G, Bradbury PA, Zhai R, Su L, Frauenhoffer CS, Hooshmand SM, De Vivo I, Lin X, Christiani DC, Kulke MH
Carcinogenesis32(8)1216-22(2011 Aug)

Differential expression of a set of genes in follicular and classic variants of papillary thyroid carcinoma.
Igci YZ, Arslan A, Akarsu E, Erkilic S, Igci M, Oztuzcu S, Cengiz B, Gogebakan B, Cakmak EA, Demiryurek AT
Endocrine pathology22(2)86-96(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that abnormal expression of DAD1 may relate to the hyperfunction of immune responses and excessive apoptosis of severe aplastic anemia CD34(+) cells
Title: Comparative proteomic analysis of CD34(+) cells in bone marrow between severe aplastic anemia and normal control.

single-nucleotide polymorphisms in DAD1 gene is associated with neuroendocrine tumor.
Title: Genetic associations with sporadic neuroendocrine tumor risk.

DAD1 gene expression is decreased in follicular variant of papillary thyroid carcinoma.
Title: Differential expression of a set of genes in follicular and classic variants of papillary thyroid carcinoma.

Observational study of gene-disease association. (HuGE Navigator)
Title: An approach based on a genome-wide association study reveals candidate loci for narcolepsy.

Observational study of gene-disease association. (HuGE Navigator)
Title: A Large-scale genetic association study of esophageal adenocarcinoma risk.

The following DAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15561	NM_001344.4 Latest version!	Homo sapiens defender against cell death 1 (DAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu15561	NM_001344.3	Homo sapiens defender against cell death 1 (DAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAD1 ( NM_001344.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335.1 Homo sapiens defender against cell death 1 (DAD1), mRNA.

Price & Availability↑

CloneID	OHu15561
Clone ID Related Accession (Same CDS sequence)	NM_001344.3 , NM_001344.4
Accession Version	NM_001344.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP198316.1, AC243965.2 and AK223129.1. On Jan 17, 2014 this sequence version replaced NM_001344.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279837.7429.1, BF312783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGT CGGTAGTGTC TGTCATTTCG CGGTTCTTAG AAGAGTACTT GAGCTCCACT 
CCGCAGCGTC TGAAGTTGCT GGACGCGTAC CTGCTGTATA TACTGCTGAC CGGGGCGCTG 
CAGTTCGGTT ACTGTCTCCT CGTGGGGACC TTCCCCTTCA ACTCTTTTCT CTCGGGCTTC 
ATCTCTTGTG TGGGGAGTTT CATCCTAGCG GTTTGCCTGA GAATACAGAT CAACCCACAG 
AACAAAGCGG ATTTCCAAGG CATCTCCCCA GAGCGAGCCT TTGCTGATTT TCTCTTTGCC 
AGCACCATCC TGCACCTTGT TGTCATGAAC TTTGTTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335.1
CDS	81..422
Translation	MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Target ORF information:

RefSeq Version	NM_001344.3
Organism	Homo sapiens(human)
Definition	Homo sapiens defender against cell death 1 (DAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344.3

ORF Insert Sequence:

ATGTCGGCGT CGGTAGTGTC TGTCATTTCG CGGTTCTTAG AAGAGTACTT GAGCTCCACT 
CCGCAGCGTC TGAAGTTGCT GGACGCGTAC CTGCTGTATA TACTGCTGAC CGGGGCGCTG 
CAGTTCGGTT ACTGTCTCCT CGTGGGGACC TTCCCCTTCA ACTCTTTTCT CTCGGGCTTC 
ATCTCTTGTG TGGGGAGTTT CATCCTAGCG GTTTGCCTGA GAATACAGAT CAACCCACAG 
AACAAAGCGG ATTTCCAAGG CATCTCCCCA GAGCGAGCCT TTGCTGATTT TCTCTTTGCC 
AGCACCATCC TGCACCTTGT TGTCATGAAC TTTGTTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAD1 ( NM_001344.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335.1 Homo sapiens defender against cell death 1 (DAD1), mRNA.

Price & Availability↑

CloneID	OHu15561
Clone ID Related Accession (Same CDS sequence)	NM_001344.3 , NM_001344.4
Accession Version	NM_001344.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP198316.1, AC243965.2 and AK223129.1. On Nov 23, 2018 this sequence version replaced NM_001344.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223129.1, SRR5189655.198726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000250498.9/ ENSP00000250498.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGCGT CGGTAGTGTC TGTCATTTCG CGGTTCTTAG AAGAGTACTT GAGCTCCACT 
CCGCAGCGTC TGAAGTTGCT GGACGCGTAC CTGCTGTATA TACTGCTGAC CGGGGCGCTG 
CAGTTCGGTT ACTGTCTCCT CGTGGGGACC TTCCCCTTCA ACTCTTTTCT CTCGGGCTTC 
ATCTCTTGTG TGGGGAGTTT CATCCTAGCG GTTTGCCTGA GAATACAGAT CAACCCACAG 
AACAAAGCGG ATTTCCAAGG CATCTCCCCA GAGCGAGCCT TTGCTGATTT TCTCTTTGCC 
AGCACCATCC TGCACCTTGT TGTCATGAAC TTTGTTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335.1
CDS	68..409
Translation	MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Target ORF information:

RefSeq Version	NM_001344.4
Organism	Homo sapiens(human)
Definition	Homo sapiens defender against cell death 1 (DAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344.4

ORF Insert Sequence:

ATGTCGGCGT CGGTAGTGTC TGTCATTTCG CGGTTCTTAG AAGAGTACTT GAGCTCCACT 
CCGCAGCGTC TGAAGTTGCT GGACGCGTAC CTGCTGTATA TACTGCTGAC CGGGGCGCTG 
CAGTTCGGTT ACTGTCTCCT CGTGGGGACC TTCCCCTTCA ACTCTTTTCT CTCGGGCTTC 
ATCTCTTGTG TGGGGAGTTT CATCCTAGCG GTTTGCCTGA GAATACAGAT CAACCCACAG 
AACAAAGCGG ATTTCCAAGG CATCTCCCCA GAGCGAGCCT TTGCTGATTT TCTCTTTGCC 
AGCACCATCC TGCACCTTGT TGTCATGAAC TTTGTTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative proteomic analysis of CD34(+) cells in bone marrow between severe aplastic anemia and normal control.
Cellular immunology304-3059-15(2016 Jun-Jul)
Qi W,Fu R,Wang H,Liu C,Ren Y,Shao Y,Shao Z

Common variants in left/right asymmetry genes and pathways are associated with relative hand skill.
PLoS genetics9(9)e1003751(2013)
Brandler WM,Morris AP,Evans DM,Scerri TS,Kemp JP,Timpson NJ,St Pourcain B,Smith GD,Ring SM,Stein J,Monaco AP,Talcott JB,Fisher SE,Webber C,Paracchini S

Genetic associations with sporadic neuroendocrine tumor risk.
Carcinogenesis32(8)1216-22(2011 Aug)
Ter-Minassian M,Wang Z,Asomaning K,Wu MC,Liu CY,Paulus JK,Liu G,Bradbury PA,Zhai R,Su L,Frauenhoffer CS,Hooshmand SM,De Vivo I,Lin X,Christiani DC,Kulke MH

Differential expression of a set of genes in follicular and classic variants of papillary thyroid carcinoma.
Endocrine pathology22(2)86-96(2011 Jun)
Igci YZ,Arslan A,Akarsu E,Erkilic S,Igci M,Oztuzcu S,Cengiz B,Gogebakan B,Cakmak EA,Demiryurek AT

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DAD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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