AP2A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP2A1
Entrez Gene ID	160
Full Name	adaptor related protein complex 2 alpha 1 subunit
Synonyms	ADTAA,AP2-ALPHA,CLAPA1
General protein information	Preferred Names adaptor related protein complex 2 alpha 1 subunit Names AP-2 complex subunit alpha-1 100 kDa coated vesicle protein A adapter-related protein complex 2 alpha-1 subunit adapter-related protein complex 2 subunit alpha-1 adaptin, alpha A adaptor protein complex AP-2 subunit alpha-1 adaptor-related protein complex 2 subunit alpha-1 alpha-adaptin A alpha1-adaptin clathrin assembly protein complex 2 alpha-A large chain clathrin-associated/assembly/adaptor protein, large, alpha 1 plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	601026

mRNA and Protein(s)

mRNA	Protein	Name
XM_011526557.2	XP_011524859.1	AP-2 complex subunit alpha-1 isoform X2
NM_014203.3	NP_055018.2	AP-2 complex subunit alpha-1 isoform 1
NM_130787.2	NP_570603.2	AP-2 complex subunit alpha-1 isoform 2
XM_011526556.2	XP_011524858.1	AP-2 complex subunit alpha-1 isoform X1
XM_011526557.3	XP_011524859.1	AP-2 complex subunit alpha-1 isoform X2
NM_014203.2	NP_055018.2	AP-2 complex subunit alpha-1 isoform 1
NM_130787.3	NP_570603.2	AP-2 complex subunit alpha-1 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AP2A1	XP_003953618.1
Xenopus tropicalis (tropical clawed frog)	ap2a1	NP_001090846.1
Homo sapiens (human)	AP2A1	NP_570603.2
Mus musculus (house mouse)	Ap2a1	NP_031484.1
Canis lupus familiaris (dog)	AP2A1	XP_003638863.1
Rattus norvegicus (Norway rat)	Ap2a1	NP_001100981.1
Danio rerio (zebrafish)	ap2a1	XP_005164119.1
	AP2A1	XP_001115452.1
Bos taurus (cattle)	AP2A1	NP_001178369.1

Gene Ontology
Bibliography

Related articles in PubMed

The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4
Jacob RA, Johnson AL, Pawlak EN, Dirk BS, Van Nynatten LR, Haeryfar SMM, Dikeakos JD
Virology5091-10(2017 09)

Central role for PICALM in amyloid-? blood-brain barrier transcytosis and clearance.
Zhao Z, Sagare AP, Ma Q, Halliday MR, Kong P, Kisler K, Winkler EA, Ramanathan A, Kanekiyo T, Bu G, Owens NC, Rege SV, Si G, Ahuja A, Zhu D, Miller CA, Schneider JA, Maeda M, Maeda T, Sugawara T, Ichida JK, Zlokovic BV
Nature neuroscience18(7)978-87(2015 Jul)

CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
Miller SE, Mathiasen S, Bright NA, Pierre F, Kelly BT, Kladt N, Schauss A, Merrifield CJ, Stamou D, H?ning S, Owen DJ
Developmental cell33(2)163-75(2015 Apr)

A role of OCRL in clathrin-coated pit dynamics and uncoating revealed by studies of Lowe syndrome cells.
N?ndez R, Balkin DM, Messa M, Liang L, Paradise S, Czapla H, Hein MY, Duncan JS, Mann M, De Camilli P
eLife3e02975(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AP-2alpha depletion enhanced apoptosis, demonstrating that disrupting the HIV-1 Nef:AP-2alpha interaction limits Nef-mediated apoptosis.
Title: The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4

These results lead to a model for the docking of the full AP-2 tetramer to membranes as bound to Nef, such that the cytosolic tail of CD4 is situated to interact with its binding site on Nef.
Title: How HIV-1 Nef hijacks the AP-2 clathrin adaptor to downregulate CD4.

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
Title: Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.

nef usurps AP-2 complexes to dysregulate Ii (invariant chain (Ii)/major histocompatibility complex class II) trafficking and potentially interfere with antigen presentation in the context of MHC-II
Title: Competition model for upregulation of the major histocompatibility complex class II-associated invariant chain by human immunodeficiency virus type 1 Nef.

AP2alpha negatively regulates HIV-1 replication in the process of nuclear translocation of viral DNA in the cytoplasm or the perinuclear region.
Title: Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome.

The following AP2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29624	NM_014203.3 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
OHu29746	NM_130787.3 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
OHu56205	XM_011526556.2 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu56206	XM_011526557.3 Latest version!	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu56206	XM_011526557.2	Homo sapiens adaptor related protein complex 2 alpha 1 subunit (AP2A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu29624	NM_014203.2	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
OHu29746	NM_130787.2	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP2A1 ( NM_014203.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055018.2 Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29624
Clone ID Related Accession (Same CDS sequence)	NM_014203.3 , NM_014203.2
Accession Version	NM_014203.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-02
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011495.8 and AC006942.1. On Jun 3, 2019 this sequence version replaced NM_014203.2. Transcript Variant: This variant (1) encodes the longer isoform of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCGCCT GCCCCCGAGA GCCCCATGGC TTTGCTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATCG GCCCTCCCAT TCCGGAAGCC 
GATGAGTTGC TGAATAAGTT TGTGTGTAAG AACAACGGGG TCCTGTTCGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC AGAGTTCCGA CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CCTCGGTGCA GTTCCAGAAT TTCTCACCCA CTGTGGTTCA CCCGGGAGAC 
CTCCAGACTC AGCTGGCTGT GCAGACCAAG CGCGTGGCGG CGCAGGTGGA CGGCGGCGCG 
CAGGTGCAGC AGGTGCTCAA TATCGAGTGC CTGCGGGACT TCCTGACGCC CCCGCTGCTG 
TCCGTGCGCT TCCGGTACGG TGGCGCCCCC CAGGCCCTCA CCCTGAAGCT CCCAGTGACC 
ATCAACAAGT TCTTCCAGCC CACCGAGATG GCGGCCCAGG ATTTCTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCTCAACA GGAGGCGCAG AAAATCTTCA AAGCCAACCA CCCCATGGAC 
GCAGAAGTTA CTAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTCCTGGA CAATGTGGAC 
CCCAACCCTG AGAACTTCGT GGGGGCGGGG ATCATCCAGA CTAAAGCCCT GCAGGTGGGC 
TGTCTGCTTC GGCTGGAGCC CAATGCCCAG GCCCAGATGT ACCGGCTGAC CCTGCGCACC 
AGCAAGGAGC CCGTCTCCCG TCACCTGTGT GAGCTGCTGG CACAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055018.2
CDS	134..3067
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_014203.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014203.3

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCGCCT GCCCCCGAGA GCCCCATGGC TTTGCTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATCG GCCCTCCCAT TCCGGAAGCC 
GATGAGTTGC TGAATAAGTT TGTGTGTAAG AACAACGGGG TCCTGTTCGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC AGAGTTCCGA CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CCTCGGTGCA GTTCCAGAAT TTCTCACCCA CTGTGGTTCA CCCGGGAGAC 
CTCCAGACTC AGCTGGCTGT GCAGACCAAG CGCGTGGCGG CGCAGGTGGA CGGCGGCGCG 
CAGGTGCAGC AGGTGCTCAA TATCGAGTGC CTGCGGGACT TCCTGACGCC CCCGCTGCTG 
TCCGTGCGCT TCCGGTACGG TGGCGCCCCC CAGGCCCTCA CCCTGAAGCT CCCAGTGACC 
ATCAACAAGT TCTTCCAGCC CACCGAGATG GCGGCCCAGG ATTTCTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCTCAACA GGAGGCGCAG AAAATCTTCA AAGCCAACCA CCCCATGGAC 
GCAGAAGTTA CTAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTCCTGGA CAATGTGGAC 
CCCAACCCTG AGAACTTCGT GGGGGCGGGG ATCATCCAGA CTAAAGCCCT GCAGGTGGGC 
TGTCTGCTTC GGCTGGAGCC CAATGCCCAG GCCCAGATGT ACCGGCTGAC CCTGCGCACC 
AGCAAGGAGC CCGTCTCCCG TCACCTGTGT GAGCTGCTGG CACAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( NM_014203.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055018.2 Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29624
Clone ID Related Accession (Same CDS sequence)	NM_014203.3 , NM_014203.2
Accession Version	NM_014203.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF289221.1. On Apr 3, 2002 this sequence version replaced NM_014203.1. Transcript Variant: This variant (1) encodes the longer isoform of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCGCCT GCCCCCGAGA GCCCCATGGC TTTGCTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATCG GCCCTCCCAT TCCGGAAGCC 
GATGAGTTGC TGAATAAGTT TGTGTGTAAG AACAACGGGG TCCTGTTCGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC AGAGTTCCGA CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CCTCGGTGCA GTTCCAGAAT TTCTCACCCA CTGTGGTTCA CCCGGGAGAC 
CTCCAGACTC AGCTGGCTGT GCAGACCAAG CGCGTGGCGG CGCAGGTGGA CGGCGGCGCG 
CAGGTGCAGC AGGTGCTCAA TATCGAGTGC CTGCGGGACT TCCTGACGCC CCCGCTGCTG 
TCCGTGCGCT TCCGGTACGG TGGCGCCCCC CAGGCCCTCA CCCTGAAGCT CCCAGTGACC 
ATCAACAAGT TCTTCCAGCC CACCGAGATG GCGGCCCAGG ATTTCTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCTCAACA GGAGGCGCAG AAAATCTTCA AAGCCAACCA CCCCATGGAC 
GCAGAAGTTA CTAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTCCTGGA CAATGTGGAC 
CCCAACCCTG AGAACTTCGT GGGGGCGGGG ATCATCCAGA CTAAAGCCCT GCAGGTGGGC 
TGTCTGCTTC GGCTGGAGCC CAATGCCCAG GCCCAGATGT ACCGGCTGAC CCTGCGCACC 
AGCAAGGAGC CCGTCTCCCG TCACCTGTGT GAGCTGCTGG CACAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055018.2
CDS	212..3145
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_014203.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014203.2

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCGCCT GCCCCCGAGA GCCCCATGGC TTTGCTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATCG GCCCTCCCAT TCCGGAAGCC 
GATGAGTTGC TGAATAAGTT TGTGTGTAAG AACAACGGGG TCCTGTTCGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC AGAGTTCCGA CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CCTCGGTGCA GTTCCAGAAT TTCTCACCCA CTGTGGTTCA CCCGGGAGAC 
CTCCAGACTC AGCTGGCTGT GCAGACCAAG CGCGTGGCGG CGCAGGTGGA CGGCGGCGCG 
CAGGTGCAGC AGGTGCTCAA TATCGAGTGC CTGCGGGACT TCCTGACGCC CCCGCTGCTG 
TCCGTGCGCT TCCGGTACGG TGGCGCCCCC CAGGCCCTCA CCCTGAAGCT CCCAGTGACC 
ATCAACAAGT TCTTCCAGCC CACCGAGATG GCGGCCCAGG ATTTCTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCTCAACA GGAGGCGCAG AAAATCTTCA AAGCCAACCA CCCCATGGAC 
GCAGAAGTTA CTAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTCCTGGA CAATGTGGAC 
CCCAACCCTG AGAACTTCGT GGGGGCGGGG ATCATCCAGA CTAAAGCCCT GCAGGTGGGC 
TGTCTGCTTC GGCTGGAGCC CAATGCCCAG GCCCAGATGT ACCGGCTGAC CCTGCGCACC 
AGCAAGGAGC CCGTCTCCCG TCACCTGTGT GAGCTGCTGG CACAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( NM_130787.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_570603.2 Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29746
Clone ID Related Accession (Same CDS sequence)	NM_130787.2 , NM_130787.3
Accession Version	NM_130787.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF289221.1. On Apr 3, 2002 this sequence version replaced NM_130787.1. Transcript Variant: This variant (2) lacks a 66 nt internal exon but maintains the reading frame, as compared to variant 1. Isoform 2 lacks 22 internal aa as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATCGGCCCTC CCATTCCGGA AGCCGATGAG 
TTGCTGAATA AGTTTGTGTG TAAGAACAAC GGGGTCCTGT TCGAGAACCA GCTGCTGCAG 
ATCGGAGTCA AGTCAGAGTT CCGACAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACCTCGG TGCAGTTCCA GAATTTCTCA CCCACTGTGG TTCACCCGGG AGACCTCCAG 
ACTCAGCTGG CTGTGCAGAC CAAGCGCGTG GCGGCGCAGG TGGACGGCGG CGCGCAGGTG 
CAGCAGGTGC TCAATATCGA GTGCCTGCGG GACTTCCTGA CGCCCCCGCT GCTGTCCGTG 
CGCTTCCGGT ACGGTGGCGC CCCCCAGGCC CTCACCCTGA AGCTCCCAGT GACCATCAAC 
AAGTTCTTCC AGCCCACCGA GATGGCGGCC CAGGATTTCT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCTC AACAGGAGGC GCAGAAAATC TTCAAAGCCA ACCACCCCAT GGACGCAGAA 
GTTACTAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTCC TGGACAATGT GGACCCCAAC 
CCTGAGAACT TCGTGGGGGC GGGGATCATC CAGACTAAAG CCCTGCAGGT GGGCTGTCTG 
CTTCGGCTGG AGCCCAATGC CCAGGCCCAG ATGTACCGGC TGACCCTGCG CACCAGCAAG 
GAGCCCGTCT CCCGTCACCT GTGTGAGCTG CTGGCACAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570603.2
CDS	212..3079
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_130787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130787.2

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATCGGCCCTC CCATTCCGGA AGCCGATGAG 
TTGCTGAATA AGTTTGTGTG TAAGAACAAC GGGGTCCTGT TCGAGAACCA GCTGCTGCAG 
ATCGGAGTCA AGTCAGAGTT CCGACAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACCTCGG TGCAGTTCCA GAATTTCTCA CCCACTGTGG TTCACCCGGG AGACCTCCAG 
ACTCAGCTGG CTGTGCAGAC CAAGCGCGTG GCGGCGCAGG TGGACGGCGG CGCGCAGGTG 
CAGCAGGTGC TCAATATCGA GTGCCTGCGG GACTTCCTGA CGCCCCCGCT GCTGTCCGTG 
CGCTTCCGGT ACGGTGGCGC CCCCCAGGCC CTCACCCTGA AGCTCCCAGT GACCATCAAC 
AAGTTCTTCC AGCCCACCGA GATGGCGGCC CAGGATTTCT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCTC AACAGGAGGC GCAGAAAATC TTCAAAGCCA ACCACCCCAT GGACGCAGAA 
GTTACTAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTCC TGGACAATGT GGACCCCAAC 
CCTGAGAACT TCGTGGGGGC GGGGATCATC CAGACTAAAG CCCTGCAGGT GGGCTGTCTG 
CTTCGGCTGG AGCCCAATGC CCAGGCCCAG ATGTACCGGC TGACCCTGCG CACCAGCAAG 
GAGCCCGTCT CCCGTCACCT GTGTGAGCTG CTGGCACAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( NM_130787.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_570603.2 Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29746
Clone ID Related Accession (Same CDS sequence)	NM_130787.2 , NM_130787.3
Accession Version	NM_130787.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011495.8 and AC006942.1. On Jun 2, 2019 this sequence version replaced NM_130787.2. Transcript Variant: This variant (2) lacks a 66 nt internal exon but maintains the reading frame, as compared to variant 1. Isoform 2 lacks 22 internal aa as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1900787.1, AL136925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATCGGCCCTC CCATTCCGGA AGCCGATGAG 
TTGCTGAATA AGTTTGTGTG TAAGAACAAC GGGGTCCTGT TCGAGAACCA GCTGCTGCAG 
ATCGGAGTCA AGTCAGAGTT CCGACAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACCTCGG TGCAGTTCCA GAATTTCTCA CCCACTGTGG TTCACCCGGG AGACCTCCAG 
ACTCAGCTGG CTGTGCAGAC CAAGCGCGTG GCGGCGCAGG TGGACGGCGG CGCGCAGGTG 
CAGCAGGTGC TCAATATCGA GTGCCTGCGG GACTTCCTGA CGCCCCCGCT GCTGTCCGTG 
CGCTTCCGGT ACGGTGGCGC CCCCCAGGCC CTCACCCTGA AGCTCCCAGT GACCATCAAC 
AAGTTCTTCC AGCCCACCGA GATGGCGGCC CAGGATTTCT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCTC AACAGGAGGC GCAGAAAATC TTCAAAGCCA ACCACCCCAT GGACGCAGAA 
GTTACTAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTCC TGGACAATGT GGACCCCAAC 
CCTGAGAACT TCGTGGGGGC GGGGATCATC CAGACTAAAG CCCTGCAGGT GGGCTGTCTG 
CTTCGGCTGG AGCCCAATGC CCAGGCCCAG ATGTACCGGC TGACCCTGCG CACCAGCAAG 
GAGCCCGTCT CCCGTCACCT GTGTGAGCTG CTGGCACAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570603.2
CDS	134..3001
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_130787.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130787.3

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CGGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAAGA GGCGGAAATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGAGA CAAAGCCTTG GATGGCTACA GTAAGAAAAA ATATGTGTGT 
AAACTGCTTT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGGCACAT GGAGGCTGTG 
AATCTGTTGA GTTCCAATAA ATACACAGAG AAGCAAATAG GTTACCTGTT CATTTCTGTG 
CTGGTGAACT CGAACTCGGA GCTGATCCGC CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGGGAGATGG GCGAGGCCTT TGCCGCTGAC ATCCCCCGCA TCCTGGTGGC CGGGGACAGC 
ATGGACAGTG TCAAGCAGAG TGCGGCCCTG TGCCTCCTTC GACTGTACAA GGCCTCGCCT 
GACCTGGTGC CCATGGGCGA GTGGACGGCG CGTGTGGTAC ACCTGCTCAA TGACCAGCAC 
ATGGGTGTGG TCACGGCCGC CGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCAGAT 
GACTTCAAGA CGTGCGTCTC TCTGGCTGTG TCGCGCCTGA GCCGGATCGT CTCCTCTGCC 
TCCACCGACC TCCAGGACTA CACCTACTAC TTCGTCCCAG CACCCTGGCT CTCGGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCT CCAGAGGATG CGGCTGTGAA GGGGCGGCTG 
GTGGAATGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CCCCCAAATC CAAGAAGGTG 
CAGCATTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCATG AAGCCGTCAA GACGCACATT GACACCGTCA TCAATGCCCT CAAGACGGAG 
CGGGACGTCA GCGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATCGTGTC GGAGATGCTG CGGTACCTGG AGACGGCAGA CTACGCCATC 
CGCGAGGAGA TCGTCCTGAA GGTGGCCATC CTGGCCGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATTGCGG GCGACTACGT GAGTGAGGAG 
GTGTGGTACC GTGTGCTACA GATCGTCACC AACCGTGATG ACGTCCAGGG CTATGCCGCC 
AAGACCGTCT TTGAGGCGCT CCAGGCCCCT GCCTGTCACG AGAACATGGT GAAGGTTGGC 
GGCTACATCC TTGGGGAGTT TGGGAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCA 
GTGCAGTTCT CCCTGCTCCA CTCCAAGTTC CATCTGTGCA GCGTGGCCAC GCGGGCGCTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCCG AGACCAAGGC CACCATCCAG 
GGCGTCCTGC GGGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTGCA GCAGCGAGCC 
GTGGAGTACC TCACCCTCAG CTCAGTGGCC AGCACCGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCGC CCTTCCCCGA GCGCGAGTCG TCCATCCTGG CCAAGCTGAA ACGCAAGAAG 
GGGCCAGGGG CCGGCAGCGC CCTGGACGAT GGCCGGAGGG ACCCCAGCAG CAACGACATC 
AACGGGGGCA TGGAGCCCAC CCCCAGCACT GTGTCGACGC CCTCGCCCTC CGCCGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCGGCA GCACCCCCGG CTTCTGCAGG AGCAGGGAAC 
CTTCTGGTGG ACGTCTTCGA TGGCCCGGCC GCCCAGCCCA GCCTGGGGCC CACCCCCGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATCGGCCCTC CCATTCCGGA AGCCGATGAG 
TTGCTGAATA AGTTTGTGTG TAAGAACAAC GGGGTCCTGT TCGAGAACCA GCTGCTGCAG 
ATCGGAGTCA AGTCAGAGTT CCGACAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACCTCGG TGCAGTTCCA GAATTTCTCA CCCACTGTGG TTCACCCGGG AGACCTCCAG 
ACTCAGCTGG CTGTGCAGAC CAAGCGCGTG GCGGCGCAGG TGGACGGCGG CGCGCAGGTG 
CAGCAGGTGC TCAATATCGA GTGCCTGCGG GACTTCCTGA CGCCCCCGCT GCTGTCCGTG 
CGCTTCCGGT ACGGTGGCGC CCCCCAGGCC CTCACCCTGA AGCTCCCAGT GACCATCAAC 
AAGTTCTTCC AGCCCACCGA GATGGCGGCC CAGGATTTCT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCTC AACAGGAGGC GCAGAAAATC TTCAAAGCCA ACCACCCCAT GGACGCAGAA 
GTTACTAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTCC TGGACAATGT GGACCCCAAC 
CCTGAGAACT TCGTGGGGGC GGGGATCATC CAGACTAAAG CCCTGCAGGT GGGCTGTCTG 
CTTCGGCTGG AGCCCAATGC CCAGGCCCAG ATGTACCGGC TGACCCTGCG CACCAGCAAG 
GAGCCCGTCT CCCGTCACCT GTGTGAGCTG CTGGCACAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( XM_011526556.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524858.1 Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X1, mRNA.

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CloneID	OHu56205
Clone ID Related Accession (Same CDS sequence)	XM_011526556.2
Accession Version	XM_011526556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC GAGGGAGGCA AGAAGGCTGG AGGGGAGGCT GCTTCACTGA CCTGAGCGAG 
CGAGCATGGG ATCTGGATGA AGTGGTCATC GTGGAGGTGC TCAAGACATG TTCTGAAGGT 
AAGAGCAAAG AGGCGGAAAT TAAGAGAATC AACAAGGAAC TGGCCAACAT CCGCTCCAAG 
TTCAAAGGAG ACAAAGCCTT GGATGGCTAC AGTAAGAAAA AATATGTGTG TAAACTGCTT 
TTCATCTTCC TGCTTGGCCA TGACATTGAC TTTGGGCACA TGGAGGCTGT GAATCTGTTG 
AGTTCCAATA AATACACAGA GAAGCAAATA GGTTACCTGT TCATTTCTGT GCTGGTGAAC 
TCGAACTCGG AGCTGATCCG CCTCATCAAC AACGCCATCA AGAATGACCT GGCCAGCCGC 
AACCCCACCT TCATGTGCCT GGCCCTGCAC TGCATCGCCA ACGTGGGCAG CCGGGAGATG 
GGCGAGGCCT TTGCCGCTGA CATCCCCCGC ATCCTGGTGG CCGGGGACAG CATGGACAGT 
GTCAAGCAGA GTGCGGCCCT GTGCCTCCTT CGACTGTACA AGGCCTCGCC TGACCTGGTG 
CCCATGGGCG AGTGGACGGC GCGTGTGGTA CACCTGCTCA ATGACCAGCA CATGGGTGTG 
GTCACGGCCG CCGTCAGCCT CATCACCTGT CTCTGCAAGA AGAACCCAGA TGACTTCAAG 
ACGTGCGTCT CTCTGGCTGT GTCGCGCCTG AGCCGGATCG TCTCCTCTGC CTCCACCGAC 
CTCCAGGACT ACACCTACTA CTTCGTCCCA GCACCCTGGC TCTCGGTGAA GCTCCTGCGG 
CTGCTGCAGT GCTACCCGCC TCCAGAGGAT GCGGCTGTGA AGGGGCGGCT GGTGGAATGT 
CTGGAGACTG TGCTCAACAA GGCCCAGGAG CCCCCCAAAT CCAAGAAGGT GCAGCATTCC 
AACGCCAAGA ACGCCATCCT CTTCGAGACC ATCAGCCTCA TCATCCACTA TGACAGTGAG 
CCCAACCTCC TGGTTCGGGC CTGCAACCAG CTGGGCCAGT TCCTGCAGCA CCGGGAGACC 
AACCTGCGCT ACCTGGCCCT GGAGAGCATG TGCACGCTGG CCAGCTCCGA GTTCTCCCAT 
GAAGCCGTCA AGACGCACAT TGACACCGTC ATCAATGCCC TCAAGACGGA GCGGGACGTC 
AGCGTGCGGC AGCGGGCGGC TGACCTCCTC TACGCCATGT GTGACCGGAG CAATGCCAAG 
CAGATCGTGT CGGAGATGCT GCGGTACCTG GAGACGGCAG ACTACGCCAT CCGCGAGGAG 
ATCGTCCTGA AGGTGGCCAT CCTGGCCGAG AAGTACGCCG TGGACTACAG CTGGTACGTG 
GACACCATCC TCAACCTCAT CCGCATTGCG GGCGACTACG TGAGTGAGGA GGTGTGGTAC 
CGTGTGCTAC AGATCGTCAC CAACCGTGAT GACGTCCAGG GCTATGCCGC CAAGACCGTC 
TTTGAGGCGC TCCAGGCCCC TGCCTGTCAC GAGAACATGG TGAAGGTTGG CGGCTACATC 
CTTGGGGAGT TTGGGAACCT GATTGCTGGG GACCCCCGCT CCAGCCCCCC AGTGCAGTTC 
TCCCTGCTCC ACTCCAAGTT CCATCTGTGC AGCGTGGCCA CGCGGGCGCT GCTGCTGTCC 
ACCTACATCA AGTTCATCAA CCTCTTCCCC GAGACCAAGG CCACCATCCA GGGCGTCCTG 
CGGGCCGGCT CCCAGCTGCG CAATGCTGAC GTGGAGCTGC AGCAGCGAGC CGTGGAGTAC 
CTCACCCTCA GCTCAGTGGC CAGCACCGAC GTCCTGGCCA CGGTGCTGGA GGAGATGCCG 
CCCTTCCCCG AGCGCGAGTC GTCCATCCTG GCCAAGCTGA AACGCAAGAA GGGGCCAGGG 
GCCGGCAGCG CCCTGGACGA TGGCCGGAGG GACCCCAGCA GCAACGACAT CAACGGGGGC 
ATGGAGCCCA CCCCCAGCAC TGTGTCGACG CCCTCGCCCT CCGCCGACCT CCTGGGGCTG 
CGGGCAGCCC CTCCCCCGGC AGCACCCCCG GCTTCTGCAG GAGCAGGGAA CCTTCTGGTG 
GACGTCTTCG ATGGCCCGGC CGCCCAGCCC AGCCTGGGGC CCACCCCCGA GGAGGCCTTC 
CTCAGCGAGC TGGAGCCGCC TGCCCCCGAG AGCCCCATGG CTTTGCTGGC TGACCCAGCT 
CCAGCTGCTG ACCCAGGTCC TGAGGACATC GGCCCTCCCA TTCCGGAAGC CGATGAGTTG 
CTGAATAAGT TTGTGTGTAA GAACAACGGG GTCCTGTTCG AGAACCAGCT GCTGCAGATC 
GGAGTCAAGT CAGAGTTCCG ACAGAACCTG GGCCGCATGT ATCTCTTCTA TGGCAACAAG 
ACCTCGGTGC AGTTCCAGAA TTTCTCACCC ACTGTGGTTC ACCCGGGAGA CCTCCAGACT 
CAGCTGGCTG TGCAGACCAA GCGCGTGGCG GCGCAGGTGG ACGGCGGCGC GCAGGTGCAG 
CAGGTGCTCA ATATCGAGTG CCTGCGGGAC TTCCTGACGC CCCCGCTGCT GTCCGTGCGC 
TTCCGGTACG GTGGCGCCCC CCAGGCCCTC ACCCTGAAGC TCCCAGTGAC CATCAACAAG 
TTCTTCCAGC CCACCGAGAT GGCGGCCCAG GATTTCTTCC AGCGCTGGAA GCAGCTGAGC 
CTCCCTCAAC AGGAGGCGCA GAAAATCTTC AAAGCCAACC ACCCCATGGA CGCAGAAGTT 
ACTAAGGCCA AGCTTCTGGG GTTTGGCTCT GCTCTCCTGG ACAATGTGGA CCCCAACCCT 
GAGAACTTCG TGGGGGCGGG GATCATCCAG ACTAAAGCCC TGCAGGTGGG CTGTCTGCTT 
CGGCTGGAGC CCAATGCCCA GGCCCAGATG TACCGGCTGA CCCTGCGCAC CAGCAAGGAG 
CCCGTCTCCC GTCACCTGTG TGAGCTGCTG GCACAGCAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524858.1
CDS	771..3755
Translation	METRGRQEGWRGGCFTDLSERAWDLDEVVIVEVLKTCSEGKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_011526556.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526556.2

ORF Insert Sequence:

ATGGAAACAC GAGGGAGGCA AGAAGGCTGG AGGGGAGGCT GCTTCACTGA CCTGAGCGAG 
CGAGCATGGG ATCTGGATGA AGTGGTCATC GTGGAGGTGC TCAAGACATG TTCTGAAGGT 
AAGAGCAAAG AGGCGGAAAT TAAGAGAATC AACAAGGAAC TGGCCAACAT CCGCTCCAAG 
TTCAAAGGAG ACAAAGCCTT GGATGGCTAC AGTAAGAAAA AATATGTGTG TAAACTGCTT 
TTCATCTTCC TGCTTGGCCA TGACATTGAC TTTGGGCACA TGGAGGCTGT GAATCTGTTG 
AGTTCCAATA AATACACAGA GAAGCAAATA GGTTACCTGT TCATTTCTGT GCTGGTGAAC 
TCGAACTCGG AGCTGATCCG CCTCATCAAC AACGCCATCA AGAATGACCT GGCCAGCCGC 
AACCCCACCT TCATGTGCCT GGCCCTGCAC TGCATCGCCA ACGTGGGCAG CCGGGAGATG 
GGCGAGGCCT TTGCCGCTGA CATCCCCCGC ATCCTGGTGG CCGGGGACAG CATGGACAGT 
GTCAAGCAGA GTGCGGCCCT GTGCCTCCTT CGACTGTACA AGGCCTCGCC TGACCTGGTG 
CCCATGGGCG AGTGGACGGC GCGTGTGGTA CACCTGCTCA ATGACCAGCA CATGGGTGTG 
GTCACGGCCG CCGTCAGCCT CATCACCTGT CTCTGCAAGA AGAACCCAGA TGACTTCAAG 
ACGTGCGTCT CTCTGGCTGT GTCGCGCCTG AGCCGGATCG TCTCCTCTGC CTCCACCGAC 
CTCCAGGACT ACACCTACTA CTTCGTCCCA GCACCCTGGC TCTCGGTGAA GCTCCTGCGG 
CTGCTGCAGT GCTACCCGCC TCCAGAGGAT GCGGCTGTGA AGGGGCGGCT GGTGGAATGT 
CTGGAGACTG TGCTCAACAA GGCCCAGGAG CCCCCCAAAT CCAAGAAGGT GCAGCATTCC 
AACGCCAAGA ACGCCATCCT CTTCGAGACC ATCAGCCTCA TCATCCACTA TGACAGTGAG 
CCCAACCTCC TGGTTCGGGC CTGCAACCAG CTGGGCCAGT TCCTGCAGCA CCGGGAGACC 
AACCTGCGCT ACCTGGCCCT GGAGAGCATG TGCACGCTGG CCAGCTCCGA GTTCTCCCAT 
GAAGCCGTCA AGACGCACAT TGACACCGTC ATCAATGCCC TCAAGACGGA GCGGGACGTC 
AGCGTGCGGC AGCGGGCGGC TGACCTCCTC TACGCCATGT GTGACCGGAG CAATGCCAAG 
CAGATCGTGT CGGAGATGCT GCGGTACCTG GAGACGGCAG ACTACGCCAT CCGCGAGGAG 
ATCGTCCTGA AGGTGGCCAT CCTGGCCGAG AAGTACGCCG TGGACTACAG CTGGTACGTG 
GACACCATCC TCAACCTCAT CCGCATTGCG GGCGACTACG TGAGTGAGGA GGTGTGGTAC 
CGTGTGCTAC AGATCGTCAC CAACCGTGAT GACGTCCAGG GCTATGCCGC CAAGACCGTC 
TTTGAGGCGC TCCAGGCCCC TGCCTGTCAC GAGAACATGG TGAAGGTTGG CGGCTACATC 
CTTGGGGAGT TTGGGAACCT GATTGCTGGG GACCCCCGCT CCAGCCCCCC AGTGCAGTTC 
TCCCTGCTCC ACTCCAAGTT CCATCTGTGC AGCGTGGCCA CGCGGGCGCT GCTGCTGTCC 
ACCTACATCA AGTTCATCAA CCTCTTCCCC GAGACCAAGG CCACCATCCA GGGCGTCCTG 
CGGGCCGGCT CCCAGCTGCG CAATGCTGAC GTGGAGCTGC AGCAGCGAGC CGTGGAGTAC 
CTCACCCTCA GCTCAGTGGC CAGCACCGAC GTCCTGGCCA CGGTGCTGGA GGAGATGCCG 
CCCTTCCCCG AGCGCGAGTC GTCCATCCTG GCCAAGCTGA AACGCAAGAA GGGGCCAGGG 
GCCGGCAGCG CCCTGGACGA TGGCCGGAGG GACCCCAGCA GCAACGACAT CAACGGGGGC 
ATGGAGCCCA CCCCCAGCAC TGTGTCGACG CCCTCGCCCT CCGCCGACCT CCTGGGGCTG 
CGGGCAGCCC CTCCCCCGGC AGCACCCCCG GCTTCTGCAG GAGCAGGGAA CCTTCTGGTG 
GACGTCTTCG ATGGCCCGGC CGCCCAGCCC AGCCTGGGGC CCACCCCCGA GGAGGCCTTC 
CTCAGCGAGC TGGAGCCGCC TGCCCCCGAG AGCCCCATGG CTTTGCTGGC TGACCCAGCT 
CCAGCTGCTG ACCCAGGTCC TGAGGACATC GGCCCTCCCA TTCCGGAAGC CGATGAGTTG 
CTGAATAAGT TTGTGTGTAA GAACAACGGG GTCCTGTTCG AGAACCAGCT GCTGCAGATC 
GGAGTCAAGT CAGAGTTCCG ACAGAACCTG GGCCGCATGT ATCTCTTCTA TGGCAACAAG 
ACCTCGGTGC AGTTCCAGAA TTTCTCACCC ACTGTGGTTC ACCCGGGAGA CCTCCAGACT 
CAGCTGGCTG TGCAGACCAA GCGCGTGGCG GCGCAGGTGG ACGGCGGCGC GCAGGTGCAG 
CAGGTGCTCA ATATCGAGTG CCTGCGGGAC TTCCTGACGC CCCCGCTGCT GTCCGTGCGC 
TTCCGGTACG GTGGCGCCCC CCAGGCCCTC ACCCTGAAGC TCCCAGTGAC CATCAACAAG 
TTCTTCCAGC CCACCGAGAT GGCGGCCCAG GATTTCTTCC AGCGCTGGAA GCAGCTGAGC 
CTCCCTCAAC AGGAGGCGCA GAAAATCTTC AAAGCCAACC ACCCCATGGA CGCAGAAGTT 
ACTAAGGCCA AGCTTCTGGG GTTTGGCTCT GCTCTCCTGG ACAATGTGGA CCCCAACCCT 
GAGAACTTCG TGGGGGCGGG GATCATCCAG ACTAAAGCCC TGCAGGTGGG CTGTCTGCTT 
CGGCTGGAGC CCAATGCCCA GGCCCAGATG TACCGGCTGA CCCTGCGCAC CAGCAAGGAG 
CCCGTCTCCC GTCACCTGTG TGAGCTGCTG GCACAGCAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( XM_011526557.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524859.1 Homo sapiens adaptor related protein complex 2 alpha 1 subunit (AP2A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu56206
Clone ID Related Accession (Same CDS sequence)	XM_011526557.2 , XM_011526557.3
Accession Version	XM_011526557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC GAGGGAGGCA AGAAGGCTGG AGGGGAGGCT GCTTCACTGA CCTGAGCGAG 
CGAGCATGGG ATCTGGATGA AGTGGTCATC GTGGAGGTGC TCAAGACATG TTCTGAAGGT 
AAGAGCAAAG AGGCGGAAAT TAAGAGAATC AACAAGGAAC TGGCCAACAT CCGCTCCAAG 
TTCAAAGGAG ACAAAGCCTT GGATGGCTAC AGTAAGAAAA AATATGTGTG TAAACTGCTT 
TTCATCTTCC TGCTTGGCCA TGACATTGAC TTTGGGCACA TGGAGGCTGT GAATCTGTTG 
AGTTCCAATA AATACACAGA GAAGCAAATA GGTTACCTGT TCATTTCTGT GCTGGTGAAC 
TCGAACTCGG AGCTGATCCG CCTCATCAAC AACGCCATCA AGAATGACCT GGCCAGCCGC 
AACCCCACCT TCATGTGCCT GGCCCTGCAC TGCATCGCCA ACGTGGGCAG CCGGGAGATG 
GGCGAGGCCT TTGCCGCTGA CATCCCCCGC ATCCTGGTGG CCGGGGACAG CATGGACAGT 
GTCAAGCAGA GTGCGGCCCT GTGCCTCCTT CGACTGTACA AGGCCTCGCC TGACCTGGTG 
CCCATGGGCG AGTGGACGGC GCGTGTGGTA CACCTGCTCA ATGACCAGCA CATGGGTGTG 
GTCACGGCCG CCGTCAGCCT CATCACCTGT CTCTGCAAGA AGAACCCAGA TGACTTCAAG 
ACGTGCGTCT CTCTGGCTGT GTCGCGCCTG AGCCGGATCG TCTCCTCTGC CTCCACCGAC 
CTCCAGGACT ACACCTACTA CTTCGTCCCA GCACCCTGGC TCTCGGTGAA GCTCCTGCGG 
CTGCTGCAGT GCTACCCGCC TCCAGAGGAT GCGGCTGTGA AGGGGCGGCT GGTGGAATGT 
CTGGAGACTG TGCTCAACAA GGCCCAGGAG CCCCCCAAAT CCAAGAAGGT GCAGCATTCC 
AACGCCAAGA ACGCCATCCT CTTCGAGACC ATCAGCCTCA TCATCCACTA TGACAGTGAG 
CCCAACCTCC TGGTTCGGGC CTGCAACCAG CTGGGCCAGT TCCTGCAGCA CCGGGAGACC 
AACCTGCGCT ACCTGGCCCT GGAGAGCATG TGCACGCTGG CCAGCTCCGA GTTCTCCCAT 
GAAGCCGTCA AGACGCACAT TGACACCGTC ATCAATGCCC TCAAGACGGA GCGGGACGTC 
AGCGTGCGGC AGCGGGCGGC TGACCTCCTC TACGCCATGT GTGACCGGAG CAATGCCAAG 
CAGATCGTGT CGGAGATGCT GCGGTACCTG GAGACGGCAG ACTACGCCAT CCGCGAGGAG 
ATCGTCCTGA AGGTGGCCAT CCTGGCCGAG AAGTACGCCG TGGACTACAG CTGGTACGTG 
GACACCATCC TCAACCTCAT CCGCATTGCG GGCGACTACG TGAGTGAGGA GGTGTGGTAC 
CGTGTGCTAC AGATCGTCAC CAACCGTGAT GACGTCCAGG GCTATGCCGC CAAGACCGTC 
TTTGAGGCGC TCCAGGCCCC TGCCTGTCAC GAGAACATGG TGAAGGTTGG CGGCTACATC 
CTTGGGGAGT TTGGGAACCT GATTGCTGGG GACCCCCGCT CCAGCCCCCC AGTGCAGTTC 
TCCCTGCTCC ACTCCAAGTT CCATCTGTGC AGCGTGGCCA CGCGGGCGCT GCTGCTGTCC 
ACCTACATCA AGTTCATCAA CCTCTTCCCC GAGACCAAGG CCACCATCCA GGGCGTCCTG 
CGGGCCGGCT CCCAGCTGCG CAATGCTGAC GTGGAGCTGC AGCAGCGAGC CGTGGAGTAC 
CTCACCCTCA GCTCAGTGGC CAGCACCGAC GTCCTGGCCA CGGTGCTGGA GGAGATGCCG 
CCCTTCCCCG AGCGCGAGTC GTCCATCCTG GCCAAGCTGA AACGCAAGAA GGGGCCAGGG 
GCCGGCAGCG CCCTGGACGA TGGCCGGAGG GACCCCAGCA GCAACGACAT CAACGGGGGC 
ATGGAGCCCA CCCCCAGCAC TGTGTCGACG CCCTCGCCCT CCGCCGACCT CCTGGGGCTG 
CGGGCAGCCC CTCCCCCGGC AGCACCCCCG GCTTCTGCAG GAGCAGGGAA CCTTCTGGTG 
GACGTCTTCG ATGGCCCGGC CGCCCAGCCC AGCCTGGGGC CCACCCCCGA GGAGGCCTTC 
CTCAGCCCAG GTCCTGAGGA CATCGGCCCT CCCATTCCGG AAGCCGATGA GTTGCTGAAT 
AAGTTTGTGT GTAAGAACAA CGGGGTCCTG TTCGAGAACC AGCTGCTGCA GATCGGAGTC 
AAGTCAGAGT TCCGACAGAA CCTGGGCCGC ATGTATCTCT TCTATGGCAA CAAGACCTCG 
GTGCAGTTCC AGAATTTCTC ACCCACTGTG GTTCACCCGG GAGACCTCCA GACTCAGCTG 
GCTGTGCAGA CCAAGCGCGT GGCGGCGCAG GTGGACGGCG GCGCGCAGGT GCAGCAGGTG 
CTCAATATCG AGTGCCTGCG GGACTTCCTG ACGCCCCCGC TGCTGTCCGT GCGCTTCCGG 
TACGGTGGCG CCCCCCAGGC CCTCACCCTG AAGCTCCCAG TGACCATCAA CAAGTTCTTC 
CAGCCCACCG AGATGGCGGC CCAGGATTTC TTCCAGCGCT GGAAGCAGCT GAGCCTCCCT 
CAACAGGAGG CGCAGAAAAT CTTCAAAGCC AACCACCCCA TGGACGCAGA AGTTACTAAG 
GCCAAGCTTC TGGGGTTTGG CTCTGCTCTC CTGGACAATG TGGACCCCAA CCCTGAGAAC 
TTCGTGGGGG CGGGGATCAT CCAGACTAAA GCCCTGCAGG TGGGCTGTCT GCTTCGGCTG 
GAGCCCAATG CCCAGGCCCA GATGTACCGG CTGACCCTGC GCACCAGCAA GGAGCCCGTC 
TCCCGTCACC TGTGTGAGCT GCTGGCACAG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524859.1
CDS	766..3684
Translation	METRGRQEGWRGGCFTDLSERAWDLDEVVIVEVLKTCSEGKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_011526557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 alpha 1 subunit (AP2A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526557.2

ORF Insert Sequence:

ATGGAAACAC GAGGGAGGCA AGAAGGCTGG AGGGGAGGCT GCTTCACTGA CCTGAGCGAG 
CGAGCATGGG ATCTGGATGA AGTGGTCATC GTGGAGGTGC TCAAGACATG TTCTGAAGGT 
AAGAGCAAAG AGGCGGAAAT TAAGAGAATC AACAAGGAAC TGGCCAACAT CCGCTCCAAG 
TTCAAAGGAG ACAAAGCCTT GGATGGCTAC AGTAAGAAAA AATATGTGTG TAAACTGCTT 
TTCATCTTCC TGCTTGGCCA TGACATTGAC TTTGGGCACA TGGAGGCTGT GAATCTGTTG 
AGTTCCAATA AATACACAGA GAAGCAAATA GGTTACCTGT TCATTTCTGT GCTGGTGAAC 
TCGAACTCGG AGCTGATCCG CCTCATCAAC AACGCCATCA AGAATGACCT GGCCAGCCGC 
AACCCCACCT TCATGTGCCT GGCCCTGCAC TGCATCGCCA ACGTGGGCAG CCGGGAGATG 
GGCGAGGCCT TTGCCGCTGA CATCCCCCGC ATCCTGGTGG CCGGGGACAG CATGGACAGT 
GTCAAGCAGA GTGCGGCCCT GTGCCTCCTT CGACTGTACA AGGCCTCGCC TGACCTGGTG 
CCCATGGGCG AGTGGACGGC GCGTGTGGTA CACCTGCTCA ATGACCAGCA CATGGGTGTG 
GTCACGGCCG CCGTCAGCCT CATCACCTGT CTCTGCAAGA AGAACCCAGA TGACTTCAAG 
ACGTGCGTCT CTCTGGCTGT GTCGCGCCTG AGCCGGATCG TCTCCTCTGC CTCCACCGAC 
CTCCAGGACT ACACCTACTA CTTCGTCCCA GCACCCTGGC TCTCGGTGAA GCTCCTGCGG 
CTGCTGCAGT GCTACCCGCC TCCAGAGGAT GCGGCTGTGA AGGGGCGGCT GGTGGAATGT 
CTGGAGACTG TGCTCAACAA GGCCCAGGAG CCCCCCAAAT CCAAGAAGGT GCAGCATTCC 
AACGCCAAGA ACGCCATCCT CTTCGAGACC ATCAGCCTCA TCATCCACTA TGACAGTGAG 
CCCAACCTCC TGGTTCGGGC CTGCAACCAG CTGGGCCAGT TCCTGCAGCA CCGGGAGACC 
AACCTGCGCT ACCTGGCCCT GGAGAGCATG TGCACGCTGG CCAGCTCCGA GTTCTCCCAT 
GAAGCCGTCA AGACGCACAT TGACACCGTC ATCAATGCCC TCAAGACGGA GCGGGACGTC 
AGCGTGCGGC AGCGGGCGGC TGACCTCCTC TACGCCATGT GTGACCGGAG CAATGCCAAG 
CAGATCGTGT CGGAGATGCT GCGGTACCTG GAGACGGCAG ACTACGCCAT CCGCGAGGAG 
ATCGTCCTGA AGGTGGCCAT CCTGGCCGAG AAGTACGCCG TGGACTACAG CTGGTACGTG 
GACACCATCC TCAACCTCAT CCGCATTGCG GGCGACTACG TGAGTGAGGA GGTGTGGTAC 
CGTGTGCTAC AGATCGTCAC CAACCGTGAT GACGTCCAGG GCTATGCCGC CAAGACCGTC 
TTTGAGGCGC TCCAGGCCCC TGCCTGTCAC GAGAACATGG TGAAGGTTGG CGGCTACATC 
CTTGGGGAGT TTGGGAACCT GATTGCTGGG GACCCCCGCT CCAGCCCCCC AGTGCAGTTC 
TCCCTGCTCC ACTCCAAGTT CCATCTGTGC AGCGTGGCCA CGCGGGCGCT GCTGCTGTCC 
ACCTACATCA AGTTCATCAA CCTCTTCCCC GAGACCAAGG CCACCATCCA GGGCGTCCTG 
CGGGCCGGCT CCCAGCTGCG CAATGCTGAC GTGGAGCTGC AGCAGCGAGC CGTGGAGTAC 
CTCACCCTCA GCTCAGTGGC CAGCACCGAC GTCCTGGCCA CGGTGCTGGA GGAGATGCCG 
CCCTTCCCCG AGCGCGAGTC GTCCATCCTG GCCAAGCTGA AACGCAAGAA GGGGCCAGGG 
GCCGGCAGCG CCCTGGACGA TGGCCGGAGG GACCCCAGCA GCAACGACAT CAACGGGGGC 
ATGGAGCCCA CCCCCAGCAC TGTGTCGACG CCCTCGCCCT CCGCCGACCT CCTGGGGCTG 
CGGGCAGCCC CTCCCCCGGC AGCACCCCCG GCTTCTGCAG GAGCAGGGAA CCTTCTGGTG 
GACGTCTTCG ATGGCCCGGC CGCCCAGCCC AGCCTGGGGC CCACCCCCGA GGAGGCCTTC 
CTCAGCCCAG GTCCTGAGGA CATCGGCCCT CCCATTCCGG AAGCCGATGA GTTGCTGAAT 
AAGTTTGTGT GTAAGAACAA CGGGGTCCTG TTCGAGAACC AGCTGCTGCA GATCGGAGTC 
AAGTCAGAGT TCCGACAGAA CCTGGGCCGC ATGTATCTCT TCTATGGCAA CAAGACCTCG 
GTGCAGTTCC AGAATTTCTC ACCCACTGTG GTTCACCCGG GAGACCTCCA GACTCAGCTG 
GCTGTGCAGA CCAAGCGCGT GGCGGCGCAG GTGGACGGCG GCGCGCAGGT GCAGCAGGTG 
CTCAATATCG AGTGCCTGCG GGACTTCCTG ACGCCCCCGC TGCTGTCCGT GCGCTTCCGG 
TACGGTGGCG CCCCCCAGGC CCTCACCCTG AAGCTCCCAG TGACCATCAA CAAGTTCTTC 
CAGCCCACCG AGATGGCGGC CCAGGATTTC TTCCAGCGCT GGAAGCAGCT GAGCCTCCCT 
CAACAGGAGG CGCAGAAAAT CTTCAAAGCC AACCACCCCA TGGACGCAGA AGTTACTAAG 
GCCAAGCTTC TGGGGTTTGG CTCTGCTCTC CTGGACAATG TGGACCCCAA CCCTGAGAAC 
TTCGTGGGGG CGGGGATCAT CCAGACTAAA GCCCTGCAGG TGGGCTGTCT GCTTCGGCTG 
GAGCCCAATG CCCAGGCCCA GATGTACCGG CTGACCCTGC GCACCAGCAA GGAGCCCGTC 
TCCCGTCACC TGTGTGAGCT GCTGGCACAG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( XM_011526557.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524859.1 Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X2, mRNA.

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CloneID	OHu56206
Clone ID Related Accession (Same CDS sequence)	XM_011526557.2 , XM_011526557.3
Accession Version	XM_011526557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	AP-2 complex subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011526557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC GAGGGAGGCA AGAAGGCTGG AGGGGAGGCT GCTTCACTGA CCTGAGCGAG 
CGAGCATGGG ATCTGGATGA AGTGGTCATC GTGGAGGTGC TCAAGACATG TTCTGAAGGT 
AAGAGCAAAG AGGCGGAAAT TAAGAGAATC AACAAGGAAC TGGCCAACAT CCGCTCCAAG 
TTCAAAGGAG ACAAAGCCTT GGATGGCTAC AGTAAGAAAA AATATGTGTG TAAACTGCTT 
TTCATCTTCC TGCTTGGCCA TGACATTGAC TTTGGGCACA TGGAGGCTGT GAATCTGTTG 
AGTTCCAATA AATACACAGA GAAGCAAATA GGTTACCTGT TCATTTCTGT GCTGGTGAAC 
TCGAACTCGG AGCTGATCCG CCTCATCAAC AACGCCATCA AGAATGACCT GGCCAGCCGC 
AACCCCACCT TCATGTGCCT GGCCCTGCAC TGCATCGCCA ACGTGGGCAG CCGGGAGATG 
GGCGAGGCCT TTGCCGCTGA CATCCCCCGC ATCCTGGTGG CCGGGGACAG CATGGACAGT 
GTCAAGCAGA GTGCGGCCCT GTGCCTCCTT CGACTGTACA AGGCCTCGCC TGACCTGGTG 
CCCATGGGCG AGTGGACGGC GCGTGTGGTA CACCTGCTCA ATGACCAGCA CATGGGTGTG 
GTCACGGCCG CCGTCAGCCT CATCACCTGT CTCTGCAAGA AGAACCCAGA TGACTTCAAG 
ACGTGCGTCT CTCTGGCTGT GTCGCGCCTG AGCCGGATCG TCTCCTCTGC CTCCACCGAC 
CTCCAGGACT ACACCTACTA CTTCGTCCCA GCACCCTGGC TCTCGGTGAA GCTCCTGCGG 
CTGCTGCAGT GCTACCCGCC TCCAGAGGAT GCGGCTGTGA AGGGGCGGCT GGTGGAATGT 
CTGGAGACTG TGCTCAACAA GGCCCAGGAG CCCCCCAAAT CCAAGAAGGT GCAGCATTCC 
AACGCCAAGA ACGCCATCCT CTTCGAGACC ATCAGCCTCA TCATCCACTA TGACAGTGAG 
CCCAACCTCC TGGTTCGGGC CTGCAACCAG CTGGGCCAGT TCCTGCAGCA CCGGGAGACC 
AACCTGCGCT ACCTGGCCCT GGAGAGCATG TGCACGCTGG CCAGCTCCGA GTTCTCCCAT 
GAAGCCGTCA AGACGCACAT TGACACCGTC ATCAATGCCC TCAAGACGGA GCGGGACGTC 
AGCGTGCGGC AGCGGGCGGC TGACCTCCTC TACGCCATGT GTGACCGGAG CAATGCCAAG 
CAGATCGTGT CGGAGATGCT GCGGTACCTG GAGACGGCAG ACTACGCCAT CCGCGAGGAG 
ATCGTCCTGA AGGTGGCCAT CCTGGCCGAG AAGTACGCCG TGGACTACAG CTGGTACGTG 
GACACCATCC TCAACCTCAT CCGCATTGCG GGCGACTACG TGAGTGAGGA GGTGTGGTAC 
CGTGTGCTAC AGATCGTCAC CAACCGTGAT GACGTCCAGG GCTATGCCGC CAAGACCGTC 
TTTGAGGCGC TCCAGGCCCC TGCCTGTCAC GAGAACATGG TGAAGGTTGG CGGCTACATC 
CTTGGGGAGT TTGGGAACCT GATTGCTGGG GACCCCCGCT CCAGCCCCCC AGTGCAGTTC 
TCCCTGCTCC ACTCCAAGTT CCATCTGTGC AGCGTGGCCA CGCGGGCGCT GCTGCTGTCC 
ACCTACATCA AGTTCATCAA CCTCTTCCCC GAGACCAAGG CCACCATCCA GGGCGTCCTG 
CGGGCCGGCT CCCAGCTGCG CAATGCTGAC GTGGAGCTGC AGCAGCGAGC CGTGGAGTAC 
CTCACCCTCA GCTCAGTGGC CAGCACCGAC GTCCTGGCCA CGGTGCTGGA GGAGATGCCG 
CCCTTCCCCG AGCGCGAGTC GTCCATCCTG GCCAAGCTGA AACGCAAGAA GGGGCCAGGG 
GCCGGCAGCG CCCTGGACGA TGGCCGGAGG GACCCCAGCA GCAACGACAT CAACGGGGGC 
ATGGAGCCCA CCCCCAGCAC TGTGTCGACG CCCTCGCCCT CCGCCGACCT CCTGGGGCTG 
CGGGCAGCCC CTCCCCCGGC AGCACCCCCG GCTTCTGCAG GAGCAGGGAA CCTTCTGGTG 
GACGTCTTCG ATGGCCCGGC CGCCCAGCCC AGCCTGGGGC CCACCCCCGA GGAGGCCTTC 
CTCAGCCCAG GTCCTGAGGA CATCGGCCCT CCCATTCCGG AAGCCGATGA GTTGCTGAAT 
AAGTTTGTGT GTAAGAACAA CGGGGTCCTG TTCGAGAACC AGCTGCTGCA GATCGGAGTC 
AAGTCAGAGT TCCGACAGAA CCTGGGCCGC ATGTATCTCT TCTATGGCAA CAAGACCTCG 
GTGCAGTTCC AGAATTTCTC ACCCACTGTG GTTCACCCGG GAGACCTCCA GACTCAGCTG 
GCTGTGCAGA CCAAGCGCGT GGCGGCGCAG GTGGACGGCG GCGCGCAGGT GCAGCAGGTG 
CTCAATATCG AGTGCCTGCG GGACTTCCTG ACGCCCCCGC TGCTGTCCGT GCGCTTCCGG 
TACGGTGGCG CCCCCCAGGC CCTCACCCTG AAGCTCCCAG TGACCATCAA CAAGTTCTTC 
CAGCCCACCG AGATGGCGGC CCAGGATTTC TTCCAGCGCT GGAAGCAGCT GAGCCTCCCT 
CAACAGGAGG CGCAGAAAAT CTTCAAAGCC AACCACCCCA TGGACGCAGA AGTTACTAAG 
GCCAAGCTTC TGGGGTTTGG CTCTGCTCTC CTGGACAATG TGGACCCCAA CCCTGAGAAC 
TTCGTGGGGG CGGGGATCAT CCAGACTAAA GCCCTGCAGG TGGGCTGTCT GCTTCGGCTG 
GAGCCCAATG CCCAGGCCCA GATGTACCGG CTGACCCTGC GCACCAGCAA GGAGCCCGTC 
TCCCGTCACC TGTGTGAGCT GCTGGCACAG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524859.1
CDS	767..3685
Translation	METRGRQEGWRGGCFTDLSERAWDLDEVVIVEVLKTCSEGKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_011526557.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526557.3

ORF Insert Sequence:

ATGGAAACAC GAGGGAGGCA AGAAGGCTGG AGGGGAGGCT GCTTCACTGA CCTGAGCGAG 
CGAGCATGGG ATCTGGATGA AGTGGTCATC GTGGAGGTGC TCAAGACATG TTCTGAAGGT 
AAGAGCAAAG AGGCGGAAAT TAAGAGAATC AACAAGGAAC TGGCCAACAT CCGCTCCAAG 
TTCAAAGGAG ACAAAGCCTT GGATGGCTAC AGTAAGAAAA AATATGTGTG TAAACTGCTT 
TTCATCTTCC TGCTTGGCCA TGACATTGAC TTTGGGCACA TGGAGGCTGT GAATCTGTTG 
AGTTCCAATA AATACACAGA GAAGCAAATA GGTTACCTGT TCATTTCTGT GCTGGTGAAC 
TCGAACTCGG AGCTGATCCG CCTCATCAAC AACGCCATCA AGAATGACCT GGCCAGCCGC 
AACCCCACCT TCATGTGCCT GGCCCTGCAC TGCATCGCCA ACGTGGGCAG CCGGGAGATG 
GGCGAGGCCT TTGCCGCTGA CATCCCCCGC ATCCTGGTGG CCGGGGACAG CATGGACAGT 
GTCAAGCAGA GTGCGGCCCT GTGCCTCCTT CGACTGTACA AGGCCTCGCC TGACCTGGTG 
CCCATGGGCG AGTGGACGGC GCGTGTGGTA CACCTGCTCA ATGACCAGCA CATGGGTGTG 
GTCACGGCCG CCGTCAGCCT CATCACCTGT CTCTGCAAGA AGAACCCAGA TGACTTCAAG 
ACGTGCGTCT CTCTGGCTGT GTCGCGCCTG AGCCGGATCG TCTCCTCTGC CTCCACCGAC 
CTCCAGGACT ACACCTACTA CTTCGTCCCA GCACCCTGGC TCTCGGTGAA GCTCCTGCGG 
CTGCTGCAGT GCTACCCGCC TCCAGAGGAT GCGGCTGTGA AGGGGCGGCT GGTGGAATGT 
CTGGAGACTG TGCTCAACAA GGCCCAGGAG CCCCCCAAAT CCAAGAAGGT GCAGCATTCC 
AACGCCAAGA ACGCCATCCT CTTCGAGACC ATCAGCCTCA TCATCCACTA TGACAGTGAG 
CCCAACCTCC TGGTTCGGGC CTGCAACCAG CTGGGCCAGT TCCTGCAGCA CCGGGAGACC 
AACCTGCGCT ACCTGGCCCT GGAGAGCATG TGCACGCTGG CCAGCTCCGA GTTCTCCCAT 
GAAGCCGTCA AGACGCACAT TGACACCGTC ATCAATGCCC TCAAGACGGA GCGGGACGTC 
AGCGTGCGGC AGCGGGCGGC TGACCTCCTC TACGCCATGT GTGACCGGAG CAATGCCAAG 
CAGATCGTGT CGGAGATGCT GCGGTACCTG GAGACGGCAG ACTACGCCAT CCGCGAGGAG 
ATCGTCCTGA AGGTGGCCAT CCTGGCCGAG AAGTACGCCG TGGACTACAG CTGGTACGTG 
GACACCATCC TCAACCTCAT CCGCATTGCG GGCGACTACG TGAGTGAGGA GGTGTGGTAC 
CGTGTGCTAC AGATCGTCAC CAACCGTGAT GACGTCCAGG GCTATGCCGC CAAGACCGTC 
TTTGAGGCGC TCCAGGCCCC TGCCTGTCAC GAGAACATGG TGAAGGTTGG CGGCTACATC 
CTTGGGGAGT TTGGGAACCT GATTGCTGGG GACCCCCGCT CCAGCCCCCC AGTGCAGTTC 
TCCCTGCTCC ACTCCAAGTT CCATCTGTGC AGCGTGGCCA CGCGGGCGCT GCTGCTGTCC 
ACCTACATCA AGTTCATCAA CCTCTTCCCC GAGACCAAGG CCACCATCCA GGGCGTCCTG 
CGGGCCGGCT CCCAGCTGCG CAATGCTGAC GTGGAGCTGC AGCAGCGAGC CGTGGAGTAC 
CTCACCCTCA GCTCAGTGGC CAGCACCGAC GTCCTGGCCA CGGTGCTGGA GGAGATGCCG 
CCCTTCCCCG AGCGCGAGTC GTCCATCCTG GCCAAGCTGA AACGCAAGAA GGGGCCAGGG 
GCCGGCAGCG CCCTGGACGA TGGCCGGAGG GACCCCAGCA GCAACGACAT CAACGGGGGC 
ATGGAGCCCA CCCCCAGCAC TGTGTCGACG CCCTCGCCCT CCGCCGACCT CCTGGGGCTG 
CGGGCAGCCC CTCCCCCGGC AGCACCCCCG GCTTCTGCAG GAGCAGGGAA CCTTCTGGTG 
GACGTCTTCG ATGGCCCGGC CGCCCAGCCC AGCCTGGGGC CCACCCCCGA GGAGGCCTTC 
CTCAGCCCAG GTCCTGAGGA CATCGGCCCT CCCATTCCGG AAGCCGATGA GTTGCTGAAT 
AAGTTTGTGT GTAAGAACAA CGGGGTCCTG TTCGAGAACC AGCTGCTGCA GATCGGAGTC 
AAGTCAGAGT TCCGACAGAA CCTGGGCCGC ATGTATCTCT TCTATGGCAA CAAGACCTCG 
GTGCAGTTCC AGAATTTCTC ACCCACTGTG GTTCACCCGG GAGACCTCCA GACTCAGCTG 
GCTGTGCAGA CCAAGCGCGT GGCGGCGCAG GTGGACGGCG GCGCGCAGGT GCAGCAGGTG 
CTCAATATCG AGTGCCTGCG GGACTTCCTG ACGCCCCCGC TGCTGTCCGT GCGCTTCCGG 
TACGGTGGCG CCCCCCAGGC CCTCACCCTG AAGCTCCCAG TGACCATCAA CAAGTTCTTC 
CAGCCCACCG AGATGGCGGC CCAGGATTTC TTCCAGCGCT GGAAGCAGCT GAGCCTCCCT 
CAACAGGAGG CGCAGAAAAT CTTCAAAGCC AACCACCCCA TGGACGCAGA AGTTACTAAG 
GCCAAGCTTC TGGGGTTTGG CTCTGCTCTC CTGGACAATG TGGACCCCAA CCCTGAGAAC 
TTCGTGGGGG CGGGGATCAT CCAGACTAAA GCCCTGCAGG TGGGCTGTCT GCTTCGGCTG 
GAGCCCAATG CCCAGGCCCA GATGTACCGG CTGACCCTGC GCACCAGCAA GGAGCCCGTC 
TCCCGTCACC TGTGTGAGCT GCTGGCACAG CAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4
Virology5091-10(2017 09)
Jacob RA,Johnson AL,Pawlak EN,Dirk BS,Van Nynatten LR,Haeryfar SMM,Dikeakos JD

Central role for PICALM in amyloid-? blood-brain barrier transcytosis and clearance.
Nature neuroscience18(7)978-87(2015 Jul)
Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
Developmental cell33(2)163-75(2015 Apr)
Miller SE,Mathiasen S,Bright NA,Pierre F,Kelly BT,Kladt N,Schauss A,Merrifield CJ,Stamou D,H?ning S,Owen DJ

A role of OCRL in clathrin-coated pit dynamics and uncoating revealed by studies of Lowe syndrome cells.
eLife3e02975(2014 Aug)
N?ndez R,Balkin DM,Messa M,Liang L,Paradise S,Czapla H,Hein MY,Duncan JS,Mann M,De Camilli P

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AP2A1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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