PRUNE2 cDNA ORF clone, Homo sapiens(human)

The following PRUNE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRUNE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu77003 NM_001308049.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu77004 NM_001308050.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu77005 NM_001308051.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu77003 XM_017014358.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu100446 NM_001330680.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98481 XM_017014354.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98482 XM_017014356.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98483 XM_017014357.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98484 XM_017014359.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu13410 NM_015225.3
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43042 NM_001308048.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43043 NM_001308047.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43031 XM_006716982.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43032 XM_006716983.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43033 XM_006716984.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43034 XM_006716985.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43035 XM_006716986.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43036 XM_005251745.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43038 XM_005251746.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43039 XM_005251748.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43040 XM_005251750.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43041 XM_005251751.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu43044 XM_005251754.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu77006 XM_011518323.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu77009 XM_011518326.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu77010 XM_011518327.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu77011 XM_011518328.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98472 XM_017014345.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98473 XM_017014346.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98474 XM_017014347.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98475 XM_017014348.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98476 XM_017014349.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98477 XM_017014350.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98478 XM_017014351.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98479 XM_017014352.1
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98480 XM_017014353.2
Latest version!
Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OHu77006 XM_011518323.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98480 XM_017014353.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98481 XM_017014354.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98482 XM_017014356.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98472 XM_017014345.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98483 XM_017014357.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu98474 XM_017014347.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu98484 XM_017014359.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu43044 XM_005251754.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu77003 XM_017014358.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu13410 NM_015225.2 Homo sapiens prune homolog 2 (PRUNE2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu77003
    Clone ID Related Accession (Same CDS sequence) NM_001308049.1 , XM_017014358.1 , XM_017014358.2
    Accession Version NM_001308049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316956.1, DC342565.1, AK296269.1, AB050197.1 and BC022571.1. Transcript Variant: This variant (4) lacks part of the 5' coding region, contains two alternate 5' exons, and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296269.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
    GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG
    GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT
    CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT
    GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC
    CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG
    GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG
    CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC
    TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT
    CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG
    TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG
    ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG
    AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG
    ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA
    GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG
    GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA
    GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294978.1
    CDS465..1478
    Translation

    Target ORF information:

    RefSeq Version NM_001308049.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
    GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG
    GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT
    CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT
    GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC
    CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG
    GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG
    CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC
    TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT
    CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG
    TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG
    ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG
    AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG
    ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA
    GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG
    GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA
    GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77004
    Clone ID Related Accession (Same CDS sequence) NM_001308050.1
    Accession Version NM_001308050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316956.1, DC342565.1, AK298805.1, AB050197.1 and BC022571.1. Transcript Variant: This variant (5) lacks part of the 5' coding region, contains an alternate 5'-terminal exon, and uses an alternate start codon, compared to variant 1. The encoded isoform (5) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.154449.1, SRR1803613.131566.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA TCCCACAGCC
    AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCCGA AGAGGAACGG
    GAGGACAACC GGCTTTGGAG GACAGTGGTC ATTGGAGAAC AAGAGCAGCG CATTGACATG
    AAGGTCATCG AGCCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294979.1
    CDS275..1333
    Translation

    Target ORF information:

    RefSeq Version NM_001308050.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA TCCCACAGCC
    AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCCGA AGAGGAACGG
    GAGGACAACC GGCTTTGGAG GACAGTGGTC ATTGGAGAAC AAGAGCAGCG CATTGACATG
    AAGGTCATCG AGCCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77005
    Clone ID Related Accession (Same CDS sequence) NM_001308051.1
    Accession Version NM_001308051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316956.1, DC342565.1, AK299857.1, AB050197.1 and BC022571.1. Transcript Variant: This variant (6) lacks part of the 5' and 3' coding region, contains an alternate 5'-terminal exon, and uses an alternate start codon and an alternate in-frame splice site, compared to variant 1. The encoded isoform (6) is shorter and has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.87777.1, AK299857.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAATATTGA CTTGAAGCTG
    AAAGAAAAGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294980.1
    CDS275..1249
    Translation

    Target ORF information:

    RefSeq Version NM_001308051.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAATATTGA CTTGAAGCTG
    AAAGAAAAGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77003
    Clone ID Related Accession (Same CDS sequence) NM_001308049.1 , XM_017014358.1 , XM_017014358.2
    Accession Version XM_017014358.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
    GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG
    GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT
    CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT
    GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC
    CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG
    GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG
    CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC
    TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT
    CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG
    TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG
    ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG
    AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG
    ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA
    GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG
    GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA
    GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869847.1
    CDS1966..2979
    Translation

    Target ORF information:

    RefSeq Version XM_017014358.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
    GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG
    GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT
    CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT
    GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC
    CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG
    GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG
    CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC
    TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT
    CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG
    TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG
    ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG
    AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG
    ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA
    GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG
    GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA
    GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77003
    Clone ID Related Accession (Same CDS sequence) NM_001308049.1 , XM_017014358.1 , XM_017014358.2
    Accession Version XM_017014358.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
    GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG
    GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT
    CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT
    GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC
    CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG
    GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG
    CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC
    TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT
    CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG
    TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG
    ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG
    AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG
    ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA
    GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG
    GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA
    GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869847.1
    CDS1687..2700
    Translation

    Target ORF information:

    RefSeq Version XM_017014358.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014358.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
    GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG
    GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT
    CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT
    GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC
    CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG
    GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG
    CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC
    TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT
    CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG
    TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG
    ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG
    AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG
    ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA
    GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG
    GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA
    GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100446
    Clone ID Related Accession (Same CDS sequence) NM_001330680.1
    Accession Version NM_001330680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359314.14 and AL161626.20. On Sep 1, 2016 this sequence version replaced XM_017014355.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21303.1, SRR1803611.155014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAACTGGA TGAAGAACTG
    AGGGAAGCAT CAGAGGCAGC TAAAACTAGC TGCCTTTACA ATGATCCAGA AATGTCTTCT
    ATGGAGAAGG ATATTGACTT GAAGCTGAAA GAAAAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317609.1
    CDS275..1336
    Translation

    Target ORF information:

    RefSeq Version NM_001330680.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAACTGGA TGAAGAACTG
    AGGGAAGCAT CAGAGGCAGC TAAAACTAGC TGCCTTTACA ATGATCCAGA AATGTCTTCT
    ATGGAGAAGG ATATTGACTT GAAGCTGAAA GAAAAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98481
    Clone ID Related Accession (Same CDS sequence) XM_017014354.2 , XM_017014354.1
    Accession Version XM_017014354.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA
    ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG
    CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869843.1
    CDS60..1157
    Translation

    Target ORF information:

    RefSeq Version XM_017014354.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014354.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA
    ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG
    CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98481
    Clone ID Related Accession (Same CDS sequence) XM_017014354.2 , XM_017014354.1
    Accession Version XM_017014354.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA
    ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG
    CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869843.1
    CDS64..1161
    Translation

    Target ORF information:

    RefSeq Version XM_017014354.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA
    ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG
    CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98482
    Clone ID Related Accession (Same CDS sequence) XM_017014356.2 , XM_017014356.1
    Accession Version XM_017014356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869845.1
    CDS60..1118
    Translation

    Target ORF information:

    RefSeq Version XM_017014356.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98482
    Clone ID Related Accession (Same CDS sequence) XM_017014356.2 , XM_017014356.1
    Accession Version XM_017014356.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869845.1
    CDS64..1122
    Translation

    Target ORF information:

    RefSeq Version XM_017014356.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98483
    Clone ID Related Accession (Same CDS sequence) XM_017014357.1 , XM_017014357.2
    Accession Version XM_017014357.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869846.1
    CDS64..1086
    Translation

    Target ORF information:

    RefSeq Version XM_017014357.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98483
    Clone ID Related Accession (Same CDS sequence) XM_017014357.1 , XM_017014357.2
    Accession Version XM_017014357.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869846.1
    CDS60..1082
    Translation

    Target ORF information:

    RefSeq Version XM_017014357.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98484
    Clone ID Related Accession (Same CDS sequence) XM_017014359.1 , XM_017014359.2
    Accession Version XM_017014359.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869848.1
    CDS64..1074
    Translation

    Target ORF information:

    RefSeq Version XM_017014359.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98484
    Clone ID Related Accession (Same CDS sequence) XM_017014359.1 , XM_017014359.2
    Accession Version XM_017014359.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869848.1
    CDS60..1070
    Translation

    Target ORF information:

    RefSeq Version XM_017014359.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
    CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG
    AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT
    CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA
    GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT
    GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA
    GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA
    CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC
    ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT
    CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC
    CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT
    GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA
    GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA
    TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT
    ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG
    CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA
    TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13410
    Clone ID Related Accession (Same CDS sequence) NM_015225.2 , NM_015225.3
    Accession Version NM_015225.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC019095.2, DA410068.1, AB050197.1, AL359314.14 and BQ008420.1. On Dec 11, 2007 this sequence version replaced NM_015225.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This RefSeq lacks full-length transcript support, but the extent of this transcript is supported by transcript alignments in human, aligning orthologous transcripts, and on functional support for the protein in PMID: 16288218. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT
    GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056040.2
    CDS125..9391
    Translation

    Target ORF information:

    RefSeq Version NM_015225.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (PRUNE2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015225.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT
    GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13410
    Clone ID Related Accession (Same CDS sequence) NM_015225.2 , NM_015225.3
    Accession Version NM_015225.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL390239.16, BC019095.2, DA410068.1, AB050197.1, AL359314.14 and BQ008420.1. On Nov 23, 2018 this sequence version replaced NM_015225.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This RefSeq lacks full-length transcript support, but the extent of this transcript is supported by transcript alignments in human, aligning orthologous transcripts, and on functional support for the protein in PMID: 16288218. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000376718.8/ ENSP00000365908.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT
    GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056040.2
    CDS152..9418
    Translation

    Target ORF information:

    RefSeq Version NM_015225.3
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015225.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT
    GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43042
    Clone ID Related Accession (Same CDS sequence) NM_001308048.1
    Accession Version NM_001308048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC019095.2, AB535152.1, AL359314.14, AB050197.1 and BC022571.1. On Apr 21, 2015 this sequence version replaced XM_005251752.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site, contains one alternate in-frame exon, and lacks two consecutive in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB535152.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA ATATTGACTT GAAGCTGAAA
    GAAAAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294977.1
    CDS125..9316
    Translation

    Target ORF information:

    RefSeq Version NM_001308048.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA ATATTGACTT GAAGCTGAAA
    GAAAAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43043
    Clone ID Related Accession (Same CDS sequence) NM_001308047.1
    Accession Version NM_001308047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9189bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC019095.2, FJ808772.1, AB535152.1, AL359314.14, CB146736.1, AB002365.2 and BC022571.1. On Apr 21, 2015 this sequence version replaced XM_005251753.1. Transcript Variant: This variant (2) contains one alternate in-frame exon and lacks two consecutive in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ808772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAATA TTGACTTGAA GCTGAAAGAA
    AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294976.1
    CDS125..9313
    Translation

    Target ORF information:

    RefSeq Version NM_001308047.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAATA TTGACTTGAA GCTGAAAGAA
    AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43031
    Clone ID Related Accession (Same CDS sequence) XM_006716982.1
    Accession Version XM_006716982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4681
    4741
    4801
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    4921
    4981
    5041
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    5161
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    5281
    5341
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    5461
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    5881
    5941
    6001
    6061
    6121
    6181
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    6301
    6361
    6421
    6481
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    6901
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7681
    7741
    7801
    7861
    7921
    7981
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    8161
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    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT
    AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGATATTGA CTTGAAGCTG
    AAAGAAAAGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717045.1
    CDS258..9572
    Translation

    Target ORF information:

    RefSeq Version XM_006716982.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT
    AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGATATTGA CTTGAAGCTG
    AAAGAAAAGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43032
    Clone ID Related Accession (Same CDS sequence) XM_006716983.1
    Accession Version XM_006716983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAGGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAGAAGG ATATTGACTT GAAGCTGAAA
    GAAAAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717046.1
    CDS258..9569
    Translation

    Target ORF information:

    RefSeq Version XM_006716983.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAGGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAGAAGG ATATTGACTT GAAGCTGAAA
    GAAAAGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43033
    Clone ID Related Accession (Same CDS sequence) XM_006716984.2
    Accession Version XM_006716984.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
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    2641
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    3181
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    4141
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    5881
    5941
    6001
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    6301
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    6421
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    7141
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    7381
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    7741
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    8401
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    8521
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    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT
    AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGAGAAGTT GTGCTGTGTT
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717047.1
    CDS258..9563
    Translation

    Target ORF information:

    RefSeq Version XM_006716984.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716984.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT
    AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGAGAAGTT GTGCTGTGTT
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43034
    Clone ID Related Accession (Same CDS sequence) XM_006716985.1
    Accession Version XM_006716985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA
    AGTGTGAGAC TGGATGAAGA ACTGAGGGAA GCATCAGAGG CAGCTAAAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTG ACTTGAAGCT GAAAGAAAAG
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717048.1
    CDS258..9563
    Translation

    Target ORF information:

    RefSeq Version XM_006716985.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA
    AGTGTGAGAC TGGATGAAGA ACTGAGGGAA GCATCAGAGG CAGCTAAAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTG ACTTGAAGCT GAAAGAAAAG
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43035
    Clone ID Related Accession (Same CDS sequence) XM_006716986.1
    Accession Version XM_006716986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1801
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    1981
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    8401
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    8701
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGACTGG ATGAAGAACT GAGGGAAGCA TCAGAGGCAG CTAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717049.1
    CDS258..9560
    Translation

    Target ORF information:

    RefSeq Version XM_006716986.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGACTGG ATGAAGAACT GAGGGAAGCA TCAGAGGCAG CTAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43036
    Clone ID Related Accession (Same CDS sequence) XM_005251745.1
    Accession Version XM_005251745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251802.1
    CDS258..9536
    Translation

    Target ORF information:

    RefSeq Version XM_005251745.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG
    GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG
    GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43038
    Clone ID Related Accession (Same CDS sequence) XM_005251746.1
    Accession Version XM_005251746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
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    1501
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    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG
    AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251803.1
    CDS258..9533
    Translation

    Target ORF information:

    RefSeq Version XM_005251746.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG
    AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43039
    Clone ID Related Accession (Same CDS sequence) XM_005251748.1
    Accession Version XM_005251748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC TATGGAGAAG
    GATATTGACT TGAAGCTGAA AGAAAAGCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251805.1
    CDS258..9530
    Translation

    Target ORF information:

    RefSeq Version XM_005251748.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC TATGGAGAAG
    GATATTGACT TGAAGCTGAA AGAAAAGCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43040
    Clone ID Related Accession (Same CDS sequence) XM_005251750.1
    Accession Version XM_005251750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
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    1921
    1981
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    4141
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    7141
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    8401
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    8581
    8641
    8701
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    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA
    AGTGTGAGAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT
    GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251807.1
    CDS258..9524
    Translation

    Target ORF information:

    RefSeq Version XM_005251750.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA
    AGTGTGAGAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT
    GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43041
    Clone ID Related Accession (Same CDS sequence) XM_005251751.1
    Accession Version XM_005251751.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGTAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251808.1
    CDS258..9485
    Translation

    Target ORF information:

    RefSeq Version XM_005251751.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGTAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43044
    Clone ID Related Accession (Same CDS sequence) XM_005251754.1 , XM_005251754.2
    Accession Version XM_005251754.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251811.1
    CDS258..9440
    Translation

    Target ORF information:

    RefSeq Version XM_005251754.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43044
    Clone ID Related Accession (Same CDS sequence) XM_005251754.1 , XM_005251754.2
    Accession Version XM_005251754.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251811.1
    CDS258..9440
    Translation

    Target ORF information:

    RefSeq Version XM_005251754.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251754.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77006
    Clone ID Related Accession (Same CDS sequence) XM_011518323.1 , XM_011518323.2
    Accession Version XM_011518323.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
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    8401
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    8641
    8701
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    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA
    GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516625.1
    CDS258..9527
    Translation

    Target ORF information:

    RefSeq Version XM_011518323.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518323.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA
    GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77006
    Clone ID Related Accession (Same CDS sequence) XM_011518323.1 , XM_011518323.2
    Accession Version XM_011518323.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA
    GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516625.1
    CDS258..9527
    Translation

    Target ORF information:

    RefSeq Version XM_011518323.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA
    GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77009
    Clone ID Related Accession (Same CDS sequence) XM_011518326.2
    Accession Version XM_011518326.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1501
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    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAGAAG
    TTGTGCTGTG TTCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516628.1
    CDS258..9515
    Translation

    Target ORF information:

    RefSeq Version XM_011518326.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAGAAG
    TTGTGCTGTG TTCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77010
    Clone ID Related Accession (Same CDS sequence) XM_011518327.1
    Accession Version XM_011518327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516629.1
    CDS258..9497
    Translation

    Target ORF information:

    RefSeq Version XM_011518327.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu77011
    Clone ID Related Accession (Same CDS sequence) XM_011518328.2
    Accession Version XM_011518328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGGAA GTTGTGCTGT GTTCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516630.1
    CDS258..9476
    Translation

    Target ORF information:

    RefSeq Version XM_011518328.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT
    TATAAGAGAA GTGTGAGGAA GTTGTGCTGT GTTCACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98472
    Clone ID Related Accession (Same CDS sequence) XM_017014345.2 , XM_017014345.1
    Accession Version XM_017014345.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG
    AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869834.1
    CDS258..9533
    Translation

    Target ORF information:

    RefSeq Version XM_017014345.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014345.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG
    AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98472
    Clone ID Related Accession (Same CDS sequence) XM_017014345.2 , XM_017014345.1
    Accession Version XM_017014345.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG
    AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869834.1
    CDS258..9533
    Translation

    Target ORF information:

    RefSeq Version XM_017014345.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG
    AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98473
    Clone ID Related Accession (Same CDS sequence) XM_017014346.1
    Accession Version XM_017014346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAGAA GGATATTGAC
    TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869835.1
    CDS258..9521
    Translation

    Target ORF information:

    RefSeq Version XM_017014346.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAGAA GGATATTGAC
    TTGAAGCTGA AAGAAAAGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98474
    Clone ID Related Accession (Same CDS sequence) XM_017014347.2 , XM_017014347.1
    Accession Version XM_017014347.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT
    CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869836.1
    CDS258..9494
    Translation

    Target ORF information:

    RefSeq Version XM_017014347.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014347.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT
    CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98474
    Clone ID Related Accession (Same CDS sequence) XM_017014347.2 , XM_017014347.1
    Accession Version XM_017014347.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT
    CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869836.1
    CDS258..9494
    Translation

    Target ORF information:

    RefSeq Version XM_017014347.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT
    CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98475
    Clone ID Related Accession (Same CDS sequence) XM_017014348.1
    Accession Version XM_017014348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAAACTAGCT GCCTTTACAA TGATCCAGAA
    ATGTCTTCTA TGGAGAAGGA TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869837.1
    CDS258..9488
    Translation

    Target ORF information:

    RefSeq Version XM_017014348.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAAACTAGCT GCCTTTACAA TGATCCAGAA
    ATGTCTTCTA TGGAGAAGGA TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98476
    Clone ID Related Accession (Same CDS sequence) XM_017014349.1
    Accession Version XM_017014349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    541
    601
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    721
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2281
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    2641
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    3181
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    3421
    3481
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    4021
    4081
    4141
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    4261
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    4381
    4441
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    4681
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    5881
    5941
    6001
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    6181
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    6301
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    6421
    6481
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    7081
    7141
    7201
    7261
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    7381
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    7621
    7681
    7741
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    7861
    7921
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    8161
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    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AGAAGGATAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869838.1
    CDS258..9485
    Translation

    Target ORF information:

    RefSeq Version XM_017014349.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AGAAGGATAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98477
    Clone ID Related Accession (Same CDS sequence) XM_017014350.1
    Accession Version XM_017014350.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGTATTGA CTTGAAGCTG AAAGAAAAGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869839.1
    CDS258..9482
    Translation

    Target ORF information:

    RefSeq Version XM_017014350.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT
    GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC
    TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG
    ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGTATTGA CTTGAAGCTG AAAGAAAAGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98478
    Clone ID Related Accession (Same CDS sequence) XM_017014351.1
    Accession Version XM_017014351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA
    AGTGTGAGTA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869840.1
    CDS258..9476
    Translation

    Target ORF information:

    RefSeq Version XM_017014351.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC
    ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG
    GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG
    ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG
    AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT
    CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA
    TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG
    GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA
    AGTGTGAGTA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98479
    Clone ID Related Accession (Same CDS sequence) XM_017014352.1
    Accession Version XM_017014352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGTATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869841.1
    CDS258..9473
    Translation

    Target ORF information:

    RefSeq Version XM_017014352.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG
    CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT
    GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT
    GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA
    TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGTATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98480
    Clone ID Related Accession (Same CDS sequence) XM_017014353.1 , XM_017014353.2
    Accession Version XM_017014353.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA
    GTAGGAAGTT ACTTATGCAC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869842.1
    CDS258..9464
    Translation

    Target ORF information:

    RefSeq Version XM_017014353.1
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA
    GTAGGAAGTT ACTTATGCAC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98480
    Clone ID Related Accession (Same CDS sequence) XM_017014353.1 , XM_017014353.2
    Accession Version XM_017014353.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein prune homolog 2 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA
    GTAGGAAGTT ACTTATGCAC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869842.1
    CDS258..9464
    Translation

    Target ORF information:

    RefSeq Version XM_017014353.2
    Organism Homo sapiens(human)
    Definition Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
    GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC
    ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT
    ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA
    GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC
    CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC
    ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA
    TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT
    AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT
    ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC
    AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG
    GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG
    GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG
    GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG
    AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT
    GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC
    ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA
    CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC
    ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC
    TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT
    GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA
    GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC
    TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG
    CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG
    CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC
    ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC
    CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT
    TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT
    GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC
    CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC
    CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG
    CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG
    AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA
    ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG
    TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA
    GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA
    AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG
    AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC
    CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC
    TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT
    TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA
    GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT
    GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA
    AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC
    AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA
    GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC
    GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC
    AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG
    GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC
    TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT
    TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG
    GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT
    CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG
    GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC
    ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA
    GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA
    TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG
    GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC
    GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT
    GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG
    AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA
    GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT
    TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC
    GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA
    GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA
    AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG
    CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG
    CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT
    AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT
    ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG
    GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT
    CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG
    GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA
    TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT
    GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC
    TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC
    ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA
    GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG
    AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC
    CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA
    CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA
    GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG
    CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG
    GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT
    GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA
    GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA
    ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG
    AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC
    AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC
    TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG
    GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT
    AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA
    GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT
    GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA
    AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT
    GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG
    AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG
    GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC
    CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG
    ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC
    GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC
    TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT
    ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA
    GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA
    GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG
    CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG
    GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC
    CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT
    AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA
    GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT
    ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG
    GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT
    CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA
    TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG
    GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT
    CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA
    TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA
    GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT
    ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT
    GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC
    TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT
    TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA
    GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG
    AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG
    GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG
    GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG
    AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG
    AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC
    AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA
    ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA
    CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT
    GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA
    ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC
    TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC
    CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT
    TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC
    CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC
    GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA
    AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC
    TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA
    GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC
    TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC
    ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA
    AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG
    ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA
    GTAGGAAGTT ACTTATGCAC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.