PRUNE2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRUNE2
Entrez Gene ID	158471
Full Name	prune homolog 2
Synonyms	BMCC1,BNIPXL,C9orf65,KIAA0367
General protein information	Preferred Names prune homolog 2 Names protein prune homolog 2 BCH motif-containing molecule at the carboxyl terminal region 1 BNIP2 motif containing molecule at the carboxyl terminal region 1 BNIP2 motif-containing molecule at the C-terminal region 1 olfaxin truncated PRUNE2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q21.2
Summary	The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015].
Disorder MIM:	610691

mRNA and Protein(s)

mRNA	Protein	Name
XM_017014353.1	XP_016869842.1	protein prune homolog 2 isoform X23
NM_015225.2	NP_056040.2	protein prune homolog 2 isoform 1
XM_005251750.1	XP_005251807.1	protein prune homolog 2 isoform X11
XM_011518326.2	XP_011516628.1	protein prune homolog 2 isoform X13
XM_017014347.2	XP_016869836.1	protein prune homolog 2 isoform X15
XM_017014354.2	XP_016869843.1	protein prune homolog 2 isoform X25
XM_017014356.2	XP_016869845.1	protein prune homolog 2 isoform X26
NM_001308050.1	NP_001294979.1	protein prune homolog 2 isoform 5
XM_017014357.1	XP_016869846.1	protein prune homolog 2 isoform X28
XM_017014359.1	XP_016869848.1	protein prune homolog 2 isoform X30
XM_011518323.1	XP_011516625.1	protein prune homolog 2 isoform X10
XM_006716986.1	XP_006717049.1	protein prune homolog 2 isoform X5
XM_005251745.1	XP_005251802.1	protein prune homolog 2 isoform X6
XM_005251746.1	XP_005251803.1	protein prune homolog 2 isoform X7
XM_017014345.2	XP_016869834.1	protein prune homolog 2 isoform X8
XM_005251748.1	XP_005251805.1	protein prune homolog 2 isoform X9
XM_011518323.2	XP_011516625.1	protein prune homolog 2 isoform X10
XM_017014346.1	XP_016869835.1	protein prune homolog 2 isoform X12
XM_017014352.1	XP_016869841.1	protein prune homolog 2 isoform X22
XM_017014353.2	XP_016869842.1	protein prune homolog 2 isoform X23
NM_001308049.1	NP_001294978.1	protein prune homolog 2 isoform 4
NM_001330680.1	NP_001317609.1	protein prune homolog 2 isoform 7
XM_017014354.1	XP_016869843.1	protein prune homolog 2 isoform X25
XM_017014356.1	XP_016869845.1	protein prune homolog 2 isoform X27
XM_017014358.1	XP_016869847.1	protein prune homolog 2 isoform X29
XM_017014345.1	XP_016869834.1	protein prune homolog 2 isoform X8
XM_006716985.1	XP_006717048.1	protein prune homolog 2 isoform X4
XM_017014357.2	XP_016869846.1	protein prune homolog 2 isoform X27
XM_017014347.1	XP_016869836.1	protein prune homolog 2 isoform X15
XM_005251754.1	XP_005251811.1	protein prune homolog 2 isoform X24
XM_006716982.1	XP_006717045.1	protein prune homolog 2 isoform X1
XM_006716984.2	XP_006717047.1	protein prune homolog 2 isoform X3
XM_017014349.1	XP_016869838.1	protein prune homolog 2 isoform X18
XM_017014350.1	XP_016869839.1	protein prune homolog 2 isoform X19
XM_011518328.2	XP_011516630.1	protein prune homolog 2 isoform X20
XM_017014351.1	XP_016869840.1	protein prune homolog 2 isoform X21
XM_017014359.2	XP_016869848.1	protein prune homolog 2 isoform X29
NM_001308051.1	NP_001294980.1	protein prune homolog 2 isoform 6
NM_001308047.1	NP_001294976.1	protein prune homolog 2 isoform 2
XM_006716983.1	XP_006717046.1	protein prune homolog 2 isoform X2
XM_011518327.1	XP_011516629.1	protein prune homolog 2 isoform X14
XM_017014348.1	XP_016869837.1	protein prune homolog 2 isoform X16
XM_005251751.1	XP_005251808.1	protein prune homolog 2 isoform X17
XM_005251754.2	XP_005251811.1	protein prune homolog 2 isoform X24
XM_017014358.2	XP_016869847.1	protein prune homolog 2 isoform X28
NM_001308048.1	NP_001294977.1	protein prune homolog 2 isoform 3
NM_015225.3	NP_056040.2	protein prune homolog 2 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRUNE2	XP_001146885.1
Mus musculus (house mouse)	Prune2	NP_851993.3
	PRUNE2	XP_002800206.1
Canis lupus familiaris (dog)	PRUNE2	XP_005615891.1
Homo sapiens (human)	PRUNE2	NP_056040.2
Bos taurus (cattle)	PRUNE2	XP_005210103.1
Rattus norvegicus (Norway rat)	Prune2	XP_006231214.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional regulation of BMCC1 mediated by E2F1 in neuroblastoma cells.
Islam MS, Tatsumi Y, Takano R, Yokochi T, Akter J, Ozaki T, Nakamura Y, Ohira M, Nakagawara A
Biochemical and biophysical research communications478(1)81-86(2016 09)

PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3.
Salameh A, Lee AK, Card?-Vila M, Nunes DN, Efstathiou E, Staquicini FI, Dobroff AS, Marchi? S, Navone NM, Hosoya H, Lauer RC, Wen S, Salmeron CC, Hoang A, Newsham I, Lima LA, Carraro DM, Oliviero S, Kolonin MG, Sidman RL, Do KA, Troncoso P, Logothetis CJ, Brentani RR, Calin GA, Cavenee WK, Dias-Neto E, Pasqualini R, Arap W
Proceedings of the National Academy of Sciences of the United States of America112(27)8403-8(2015 Jul)

Identification and diagnostic performance of a small RNA within the PCA3 and BMCC1 gene locus that potentially targets mRNA.
Drayton RM, Rehman I, Clarke R, Zhao Z, Pang K, Miah S, Stoehr R, Hartmann A, Blizard S, Lavin M, Bryant HE, Martens-Uzunova ES, Jenster G, Hamdy FC, Gardiner RA, Catto JW
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology24(1)268-75(2015 Jan)

BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.
Tatsumi Y, Takano R, Islam MS, Yokochi T, Itami M, Nakamura Y, Nakagawara A
Cell death & disease6e1607(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E2F1 directly facilitated BMCC1 transcription. These results also suggest that BMCC1 induced by E2F1 acts as a tumor suppressor through its pro-apoptotic function, resulted in favorable prognosis of neuroblastoma.
Title: Transcriptional regulation of BMCC1 mediated by E2F1 in neuroblastoma cells.

Results show prostate cancer antigen 3 (PCA3) as a dominant-negative oncogene and PRUNE2 protein as an unrecognized tumor suppressor gene in prostate cancer.
Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3.

recurrent germ line and somatic mutations in PRUNE2 were found in parathyroid carcinoma and computationally predicted to be deleterious; in addition, recurrent mutations in kinase genes related to cell migration and invasion were found.
Title: Whole-exome sequencing studies of parathyroid carcinomas reveal novel PRUNE2 mutations, distinctive mutational spectra related to APOBEC-catalyzed DNA mutagenesis and mutational enrichment in kinases associated with cell migration and invasion.

BMCC1 negatively regulates phosphorylation pathway of AKT, resulted in apoptosis and the BNIP2 homology region of BMCC1 interacts with BCL2.
Title: BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.

BMCC1 is an AP-2 associated endosomal protein in prostate cancer cells.
Title: BMCC1 is an AP-2 associated endosomal protein in prostate cancer cells.

The following PRUNE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRUNE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu77003	NM_001308049.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu77004	NM_001308050.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu77005	NM_001308051.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu77003	XM_017014358.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu100446	NM_001330680.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98481	XM_017014354.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98482	XM_017014356.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98483	XM_017014357.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98484	XM_017014359.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu13410	NM_015225.3 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43042	NM_001308048.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43043	NM_001308047.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43031	XM_006716982.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43032	XM_006716983.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43033	XM_006716984.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43034	XM_006716985.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43035	XM_006716986.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43036	XM_005251745.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43038	XM_005251746.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43039	XM_005251748.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43040	XM_005251750.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43041	XM_005251751.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu43044	XM_005251754.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu77006	XM_011518323.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu77009	XM_011518326.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu77010	XM_011518327.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu77011	XM_011518328.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98472	XM_017014345.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98473	XM_017014346.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98474	XM_017014347.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98475	XM_017014348.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98476	XM_017014349.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98477	XM_017014350.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98478	XM_017014351.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98479	XM_017014352.1 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98480	XM_017014353.2 Latest version!	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu77006	XM_011518323.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98480	XM_017014353.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98481	XM_017014354.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98482	XM_017014356.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98472	XM_017014345.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98483	XM_017014357.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98474	XM_017014347.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu98484	XM_017014359.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu43044	XM_005251754.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu77003	XM_017014358.1	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu13410	NM_015225.2	Homo sapiens prune homolog 2 (PRUNE2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRUNE2 ( NM_001308049.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294978.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu77003
Clone ID Related Accession (Same CDS sequence)	NM_001308049.1 , XM_017014358.1 , XM_017014358.2
Accession Version	NM_001308049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316956.1, DC342565.1, AK296269.1, AB050197.1 and BC022571.1. Transcript Variant: This variant (4) lacks part of the 5' coding region, contains two alternate 5' exons, and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296269.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG 
GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT 
CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT 
GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC 
CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG 
GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG 
CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC 
TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT 
CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG 
TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG 
ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG 
AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG 
ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA 
GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG 
GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA 
GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294978.1
CDS	465..1478
Translation	MNGLRNKQLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	NM_001308049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308049.1

ORF Insert Sequence:

ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG 
GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT 
CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT 
GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC 
CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG 
GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG 
CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC 
TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT 
CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG 
TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG 
ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG 
AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG 
ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA 
GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG 
GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA 
GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_001308050.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294979.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 5, mRNA.

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CloneID	OHu77004
Clone ID Related Accession (Same CDS sequence)	NM_001308050.1
Accession Version	NM_001308050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316956.1, DC342565.1, AK298805.1, AB050197.1 and BC022571.1. Transcript Variant: This variant (5) lacks part of the 5' coding region, contains an alternate 5'-terminal exon, and uses an alternate start codon, compared to variant 1. The encoded isoform (5) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.154449.1, SRR1803613.131566.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA TCCCACAGCC 
AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCCGA AGAGGAACGG 
GAGGACAACC GGCTTTGGAG GACAGTGGTC ATTGGAGAAC AAGAGCAGCG CATTGACATG 
AAGGTCATCG AGCCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG 
GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294979.1
CDS	275..1333
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	NM_001308050.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308050.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA TCCCACAGCC 
AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCCGA AGAGGAACGG 
GAGGACAACC GGCTTTGGAG GACAGTGGTC ATTGGAGAAC AAGAGCAGCG CATTGACATG 
AAGGTCATCG AGCCCTACAG GAGAGTCATT TCTCACGGAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG 
GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_001308051.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294980.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu77005
Clone ID Related Accession (Same CDS sequence)	NM_001308051.1
Accession Version	NM_001308051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316956.1, DC342565.1, AK299857.1, AB050197.1 and BC022571.1. Transcript Variant: This variant (6) lacks part of the 5' and 3' coding region, contains an alternate 5'-terminal exon, and uses an alternate start codon and an alternate in-frame splice site, compared to variant 1. The encoded isoform (6) is shorter and has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.87777.1, AK299857.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAATATTGA CTTGAAGCTG 
AAAGAAAAGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294980.1
CDS	275..1249
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIINIDLKLKEKP

Target ORF information:

RefSeq Version	NM_001308051.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308051.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAATATTGA CTTGAAGCTG 
AAAGAAAAGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014358.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869847.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OHu77003
Clone ID Related Accession (Same CDS sequence)	NM_001308049.1 , XM_017014358.1 , XM_017014358.2
Accession Version	XM_017014358.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG 
GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT 
CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT 
GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC 
CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG 
GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG 
CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC 
TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT 
CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG 
TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG 
ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG 
AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG 
ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA 
GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG 
GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA 
GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869847.1
CDS	1966..2979
Translation	MNGLRNKQLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014358.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014358.1

ORF Insert Sequence:

ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG 
GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT 
CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT 
GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC 
CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG 
GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG 
CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC 
TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT 
CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG 
TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG 
ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG 
AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG 
ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA 
GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG 
GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA 
GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014358.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869847.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OHu77003
Clone ID Related Accession (Same CDS sequence)	NM_001308049.1 , XM_017014358.1 , XM_017014358.2
Accession Version	XM_017014358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG 
GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT 
CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT 
GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC 
CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG 
GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG 
CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC 
TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT 
CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG 
TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG 
ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG 
AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG 
ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA 
GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG 
GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA 
GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869847.1
CDS	1687..2700
Translation	MNGLRNKQLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014358.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014358.2

ORF Insert Sequence:

ATGAATGGCC TGAGAAACAA ACAACTACTG TCAGAGGATG TAGGAATGGA CATCCCCTTT 
GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC CTGAACCTCC TAATTCTCTG 
GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG CCCCAAATAT CAATCTTTCT 
CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT TGGACAGTCC AGATGAAATT 
GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG ATTCTTTTGA GTACACTGGC 
CATGATCCCA CAGCCAACAA AGATTCTGGC CAAGAGTCAG AGTCTATTCC AGAATATACG 
GCCGAAGAGG AACGGGAGGA CAACCGGCTT TGGAGGACAG TGGTCATTGG AGAACAAGAG 
CAGCGCATTG ACATGAAGGT CATCGAGCCC TACAGGAGAG TCATTTCTCA CGGAGGATAC 
TATGGGGACG GTCTAAATGC CATCATTGTG TTTGCCGCCT GTTTTCTGCC AGACAGCAGT 
CGGGCGGATT ACCACTATGT CATGGAAAAT CTTTTCCTAT ATGTAATAAG TACTTTAGAG 
TTGATGGTAG CTGAAGACTA TATGATTGTG TACTTGAATG GTGCAACCCC AAGAAGGAGG 
ATGCCAGGGC TAGGCTGGAT GAAGAAATGC TACCAGATGA TTGACAGACG GTTGAGGAAG 
AATTTGAAAT CATTCATCAT TGTTCATCCA TCTTGGTTCA TCAGAACAAT CCTTGCTGTG 
ACACGACCTT TTATAAGTTC AAAATTCAGC AGTAAAATTA AATATGTCAA TAGCTTATCA 
GAACTCAGTG GGCTGATCCC AATGGATTGC ATCCACATTC CAGAGAGCAT CATCAAACTG 
GATGAAGAAC TGAGGGAAGC ATCAGAGGCA GCTAAAACTA GCTGCCTTTA CAATGATCCA 
GAAATGTCTT CTATGGAGAA GGATATTGAC TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_001330680.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317609.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu100446
Clone ID Related Accession (Same CDS sequence)	NM_001330680.1
Accession Version	NM_001330680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359314.14 and AL161626.20. On Sep 1, 2016 this sequence version replaced XM_017014355.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21303.1, SRR1803611.155014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAACTGGA TGAAGAACTG 
AGGGAAGCAT CAGAGGCAGC TAAAACTAGC TGCCTTTACA ATGATCCAGA AATGTCTTCT 
ATGGAGAAGG ATATTGACTT GAAGCTGAAA GAAAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317609.1
CDS	275..1336
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	NM_001330680.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330680.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAACTGGA TGAAGAACTG 
AGGGAAGCAT CAGAGGCAGC TAAAACTAGC TGCCTTTACA ATGATCCAGA AATGTCTTCT 
ATGGAGAAGG ATATTGACTT GAAGCTGAAA GAAAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014354.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869843.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OHu98481
Clone ID Related Accession (Same CDS sequence)	XM_017014354.2 , XM_017014354.1
Accession Version	XM_017014354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA 
ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG 
CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869843.1
CDS	60..1157
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014354.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014354.2

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA 
ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG 
CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014354.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869843.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OHu98481
Clone ID Related Accession (Same CDS sequence)	XM_017014354.2 , XM_017014354.1
Accession Version	XM_017014354.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA 
ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG 
CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869843.1
CDS	64..1161
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014354.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014354.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG ACTGGATGAA GAACTGAGGG AAGCATCAGA GGCAGCTAAA 
ACTAGCTGCC TTTACAATGA TCCAGAAATG TCTTCTATGG AGAAGGATAT TGACTTGAAG 
CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014356.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869845.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OHu98482
Clone ID Related Accession (Same CDS sequence)	XM_017014356.2 , XM_017014356.1
Accession Version	XM_017014356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869845.1
CDS	60..1118
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014356.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014356.2

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014356.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869845.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OHu98482
Clone ID Related Accession (Same CDS sequence)	XM_017014356.2 , XM_017014356.1
Accession Version	XM_017014356.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869845.1
CDS	64..1122
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014356.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014356.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014357.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869846.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OHu98483
Clone ID Related Accession (Same CDS sequence)	XM_017014357.1 , XM_017014357.2
Accession Version	XM_017014357.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC 
AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869846.1
CDS	64..1086
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014357.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014357.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC 
AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014357.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869846.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OHu98483
Clone ID Related Accession (Same CDS sequence)	XM_017014357.1 , XM_017014357.2
Accession Version	XM_017014357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC 
AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869846.1
CDS	60..1082
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014357.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014357.2

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAAACTAG CTGCCTTTAC 
AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014359.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869848.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OHu98484
Clone ID Related Accession (Same CDS sequence)	XM_017014359.1 , XM_017014359.2
Accession Version	XM_017014359.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869848.1
CDS	64..1074
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVSIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014359.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014359.1

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014359.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869848.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OHu98484
Clone ID Related Accession (Same CDS sequence)	XM_017014359.1 , XM_017014359.2
Accession Version	XM_017014359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869848.1
CDS	60..1070
Translation	MLKSCSRASFSPSVRKPPLILRRLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVSIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014359.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014359.2

ORF Insert Sequence:

ATGCTGAAGA GCTGTAGTCG TGCATCCTTC TCACCCTCCG TTAGAAAGCC TCCTCTCATC 
CTCAGAAGAC TACTGTCAGA GGATGTAGGA ATGGACATCC CCTTTGAAGA GGGCGTGCTG 
AGTCCCAGTG CTGCAGACAT GAGGCCTGAA CCTCCTAATT CTCTGGATCT TAATGACACT 
CATCCTCGGA GAATCAAGCT CACAGCCCCA AATATCAATC TTTCTCTGGA CCAAAGTGAA 
GGATCTATTC TCTCTGATGA TAACTTGGAC AGTCCAGATG AAATTGACAT CAATGTGGAT 
GAACTTGATA CCCCCGATGA AGCAGATTCT TTTGAGTACA CTGGCCATGA AGATCCCACA 
GCCAACAAAG ATTCTGGCCA AGAGTCAGAG TCTATTCCAG AATATACGGC CGAAGAGGAA 
CGGGAGGACA ACCGGCTTTG GAGGACAGTG GTCATTGGAG AACAAGAGCA GCGCATTGAC 
ATGAAGGTCA TCGAGCCCTA CAGGAGAGTC ATTTCTCACG GAGGATACTA TGGGGACGGT 
CTAAATGCCA TCATTGTGTT TGCCGCCTGT TTTCTGCCAG ACAGCAGTCG GGCGGATTAC 
CACTATGTCA TGGAAAATCT TTTCCTATAT GTAATAAGTA CTTTAGAGTT GATGGTAGCT 
GAAGACTATA TGATTGTGTA CTTGAATGGT GCAACCCCAA GAAGGAGGAT GCCAGGGCTA 
GGCTGGATGA AGAAATGCTA CCAGATGATT GACAGACGGT TGAGGAAGAA TTTGAAATCA 
TTCATCATTG TTCATCCATC TTGGTTCATC AGAACAATCC TTGCTGTGAC ACGACCTTTT 
ATAAGTTCAA AATTCAGCAG TAAAATTAAA TATGTCAATA GCTTATCAGA ACTCAGTGGG 
CTGATCCCAA TGGATTGCAT CCACATTCCA GAGAGCATCA TCAAGTACGA TGAAGAGAGA 
TCTTATAAGA GAAGTGTGAG TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_015225.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056040.2 Homo sapiens prune homolog 2 (PRUNE2), transcript variant 1, mRNA.

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CloneID	OHu13410
Clone ID Related Accession (Same CDS sequence)	NM_015225.2 , NM_015225.3
Accession Version	NM_015225.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC019095.2, DA410068.1, AB050197.1, AL359314.14 and BQ008420.1. On Dec 11, 2007 this sequence version replaced NM_015225.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This RefSeq lacks full-length transcript support, but the extent of this transcript is supported by transcript alignments in human, aligning orthologous transcripts, and on functional support for the protein in PMID: 16288218. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG 
GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT 
GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056040.2
CDS	125..9391
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	NM_015225.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (PRUNE2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015225.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG 
GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT 
GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_015225.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056040.2 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 1, mRNA.

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CloneID	OHu13410
Clone ID Related Accession (Same CDS sequence)	NM_015225.2 , NM_015225.3
Accession Version	NM_015225.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL390239.16, BC019095.2, DA410068.1, AB050197.1, AL359314.14 and BQ008420.1. On Nov 23, 2018 this sequence version replaced NM_015225.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This RefSeq lacks full-length transcript support, but the extent of this transcript is supported by transcript alignments in human, aligning orthologous transcripts, and on functional support for the protein in PMID: 16288218. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000376718.8/ ENSP00000365908.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG 
GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT 
GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056040.2
CDS	152..9418
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	NM_015225.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015225.3

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAG 
GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT 
GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_001308048.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294977.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 3, mRNA.

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CloneID	OHu43042
Clone ID Related Accession (Same CDS sequence)	NM_001308048.1
Accession Version	NM_001308048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC019095.2, AB535152.1, AL359314.14, AB050197.1 and BC022571.1. On Apr 21, 2015 this sequence version replaced XM_005251752.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site, contains one alternate in-frame exon, and lacks two consecutive in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB535152.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA ATATTGACTT GAAGCTGAAA 
GAAAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294977.1
CDS	125..9316
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIINIDLKLKEKP

Target ORF information:

RefSeq Version	NM_001308048.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308048.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA ATATTGACTT GAAGCTGAAA 
GAAAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( NM_001308047.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294976.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 2, mRNA.

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CloneID	OHu43043
Clone ID Related Accession (Same CDS sequence)	NM_001308047.1
Accession Version	NM_001308047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC019095.2, FJ808772.1, AB535152.1, AL359314.14, CB146736.1, AB002365.2 and BC022571.1. On Apr 21, 2015 this sequence version replaced XM_005251753.1. Transcript Variant: This variant (2) contains one alternate in-frame exon and lacks two consecutive in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ808772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAATA TTGACTTGAA GCTGAAAGAA 
AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294976.1
CDS	125..9313
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIINIDLKLKEKP

Target ORF information:

RefSeq Version	NM_001308047.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308047.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAATA TTGACTTGAA GCTGAAAGAA 
AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_006716982.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717045.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X1, mRNA.

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CloneID	OHu43031
Clone ID Related Accession (Same CDS sequence)	XM_006716982.1
Accession Version	XM_006716982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT 
AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGATATTGA CTTGAAGCTG 
AAAGAAAAGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717045.1
CDS	258..9572
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_006716982.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716982.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT 
AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGATATTGA CTTGAAGCTG 
AAAGAAAAGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_006716983.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717046.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X2, mRNA.

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CloneID	OHu43032
Clone ID Related Accession (Same CDS sequence)	XM_006716983.1
Accession Version	XM_006716983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT 
AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAGGCAGC TAAAACTAGC 
TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAGAAGG ATATTGACTT GAAGCTGAAA 
GAAAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717046.1
CDS	258..9569
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_006716983.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716983.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT 
AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAGGCAGC TAAAACTAGC 
TGCCTTTACA ATGATCCAGA AATGTCTTCT ATGGAGAAGG ATATTGACTT GAAGCTGAAA 
GAAAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_006716984.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717047.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X4, mRNA.

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CloneID	OHu43033
Clone ID Related Accession (Same CDS sequence)	XM_006716984.2
Accession Version	XM_006716984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT 
AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGAGAAGTT GTGCTGTGTT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717047.1
CDS	258..9563
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKEKLCCVH

Target ORF information:

RefSeq Version	XM_006716984.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716984.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAAACT 
AGCTGCCTTT ACAATGATCC AGAAATGTCT TCTATGGAGA AGGAGAAGTT GTGCTGTGTT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_006716985.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717048.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X5, mRNA.

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CloneID	OHu43034
Clone ID Related Accession (Same CDS sequence)	XM_006716985.1
Accession Version	XM_006716985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA 
AGTGTGAGAC TGGATGAAGA ACTGAGGGAA GCATCAGAGG CAGCTAAAAC TAGCTGCCTT 
TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTG ACTTGAAGCT GAAAGAAAAG 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717048.1
CDS	258..9563
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_006716985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716985.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA 
AGTGTGAGAC TGGATGAAGA ACTGAGGGAA GCATCAGAGG CAGCTAAAAC TAGCTGCCTT 
TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTG ACTTGAAGCT GAAAGAAAAG 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_006716986.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717049.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X6, mRNA.

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CloneID	OHu43035
Clone ID Related Accession (Same CDS sequence)	XM_006716986.1
Accession Version	XM_006716986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT 
GTGAGACTGG ATGAAGAACT GAGGGAAGCA TCAGAGGCAG CTAAAACTAG CTGCCTTTAC 
AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717049.1
CDS	258..9560
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_006716986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716986.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT 
GTGAGACTGG ATGAAGAACT GAGGGAAGCA TCAGAGGCAG CTAAAACTAG CTGCCTTTAC 
AATGATCCAG AAATGTCTTC TATGGAGAAG GATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251745.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251802.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X7, mRNA.

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CloneID	OHu43036
Clone ID Related Accession (Same CDS sequence)	XM_005251745.1
Accession Version	XM_005251745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG 
GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251802.1
CDS	258..9536
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251745.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251745.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AACTGGATGA AGAACTGAGG 
GAAGCATCAG AGGCAGCTAA AACTAGCTGC CTTTACAATG ATCCAGAAAT GTCTTCTATG 
GAGAAGGATA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251746.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251803.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X8, mRNA.

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CloneID	OHu43038
Clone ID Related Accession (Same CDS sequence)	XM_005251746.1
Accession Version	XM_005251746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG 
AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251803.1
CDS	258..9533
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251746.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251746.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG 
AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251748.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251805.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X10, mRNA.

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CloneID	OHu43039
Clone ID Related Accession (Same CDS sequence)	XM_005251748.1
Accession Version	XM_005251748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT 
AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC TATGGAGAAG 
GATATTGACT TGAAGCTGAA AGAAAAGCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251805.1
CDS	258..9530
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251748.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT 
AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC TATGGAGAAG 
GATATTGACT TGAAGCTGAA AGAAAAGCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251750.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251807.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X13, mRNA.

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CloneID	OHu43040
Clone ID Related Accession (Same CDS sequence)	XM_005251750.1
Accession Version	XM_005251750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA 
AGTGTGAGAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT 
GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251807.1
CDS	258..9524
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251750.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251750.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA 
AGTGTGAGAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCTATGGA GAAGGATATT 
GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251751.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251808.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X20, mRNA.

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CloneID	OHu43041
Clone ID Related Accession (Same CDS sequence)	XM_005251751.1
Accession Version	XM_005251751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGTAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251808.1
CDS	258..9485
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVSIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251751.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251751.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGTAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251754.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251811.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X28, mRNA.

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CloneID	OHu43044
Clone ID Related Accession (Same CDS sequence)	XM_005251754.1 , XM_005251754.2
Accession Version	XM_005251754.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251811.1
CDS	258..9440
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIINIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251754.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251754.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_005251754.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251811.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X28, mRNA.

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CloneID	OHu43044
Clone ID Related Accession (Same CDS sequence)	XM_005251754.1 , XM_005251754.2
Accession Version	XM_005251754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251811.1
CDS	258..9440
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIINIDLKLKEKP

Target ORF information:

RefSeq Version	XM_005251754.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251754.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AATATTGACT TGAAGCTGAA AGAAAAGCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_011518323.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516625.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X11, mRNA.

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CloneID	OHu77006
Clone ID Related Accession (Same CDS sequence)	XM_011518323.1 , XM_011518323.2
Accession Version	XM_011518323.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA 
GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT 
ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516625.1
CDS	258..9527
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_011518323.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518323.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA 
GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT 
ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_011518323.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516625.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X11, mRNA.

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CloneID	OHu77006
Clone ID Related Accession (Same CDS sequence)	XM_011518323.1 , XM_011518323.2
Accession Version	XM_011518323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA 
GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT 
ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516625.1
CDS	258..9527
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_011518323.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518323.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAACTGGATG AAGAACTGAG GGAAGCATCA 
GAGGCAGCTA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT 
ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_011518326.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516628.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X16, mRNA.

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CloneID	OHu77009
Clone ID Related Accession (Same CDS sequence)	XM_011518326.2
Accession Version	XM_011518326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAGAAG 
TTGTGCTGTG TTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516628.1
CDS	258..9515
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRLDEELREASEAAKKLCCVH

Target ORF information:

RefSeq Version	XM_011518326.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518326.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGACT GGATGAAGAA CTGAGGGAAG CATCAGAGGC AGCTAAGAAG 
TTGTGCTGTG TTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_011518327.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516629.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X17, mRNA.

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CloneID	OHu77010
Clone ID Related Accession (Same CDS sequence)	XM_011518327.1
Accession Version	XM_011518327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AAACTAGCTG CCTTTACAAT 
GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516629.1
CDS	258..9497
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_011518327.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518327.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AAACTAGCTG CCTTTACAAT 
GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTGACTTGA AGCTGAAAGA AAAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_011518328.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516630.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X24, mRNA.

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CloneID	OHu77011
Clone ID Related Accession (Same CDS sequence)	XM_011518328.2
Accession Version	XM_011518328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGGAA GTTGTGCTGT GTTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516630.1
CDS	258..9476
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRKLCCVH

Target ORF information:

RefSeq Version	XM_011518328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518328.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AGTACGATGA AGAGAGATCT 
TATAAGAGAA GTGTGAGGAA GTTGTGCTGT GTTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014345.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869834.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X9, mRNA.

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CloneID	OHu98472
Clone ID Related Accession (Same CDS sequence)	XM_017014345.2 , XM_017014345.1
Accession Version	XM_017014345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA 
GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG 
AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869834.1
CDS	258..9533
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014345.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014345.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA 
GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG 
AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014345.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869834.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA.

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CloneID	OHu98472
Clone ID Related Accession (Same CDS sequence)	XM_017014345.2 , XM_017014345.1
Accession Version	XM_017014345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA 
GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG 
AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869834.1
CDS	258..9533
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014345.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA 
GCATCAGAGG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCTATGGAG 
AAGGATATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014346.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869835.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X14, mRNA.

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CloneID	OHu98473
Clone ID Related Accession (Same CDS sequence)	XM_017014346.1
Accession Version	XM_017014346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT 
GTGAGAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAGAA GGATATTGAC 
TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869835.1
CDS	258..9521
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVRTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014346.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT 
GTGAGAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CTATGGAGAA GGATATTGAC 
TTGAAGCTGA AAGAAAAGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014347.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869836.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X18, mRNA.

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CloneID	OHu98474
Clone ID Related Accession (Same CDS sequence)	XM_017014347.2 , XM_017014347.1
Accession Version	XM_017014347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT 
CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869836.1
CDS	258..9494
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014347.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT 
CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014347.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869836.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X18, mRNA.

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CloneID	OHu98474
Clone ID Related Accession (Same CDS sequence)	XM_017014347.2 , XM_017014347.1
Accession Version	XM_017014347.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT 
CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869836.1
CDS	258..9494
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014347.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAAA CTAGCTGCCT TTACAATGAT 
CCAGAAATGT CTTCTATGGA GAAGGATATT GACTTGAAGC TGAAAGAAAA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014348.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869837.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X19, mRNA.

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CloneID	OHu98475
Clone ID Related Accession (Same CDS sequence)	XM_017014348.1
Accession Version	XM_017014348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAAACTAGCT GCCTTTACAA TGATCCAGAA 
ATGTCTTCTA TGGAGAAGGA TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869837.1
CDS	258..9488
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014348.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014348.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAAACTAGCT GCCTTTACAA TGATCCAGAA 
ATGTCTTCTA TGGAGAAGGA TATTGACTTG AAGCTGAAAG AAAAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014349.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869838.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X21, mRNA.

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CloneID	OHu98476
Clone ID Related Accession (Same CDS sequence)	XM_017014349.1
Accession Version	XM_017014349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG 
TCTTCTATGG AGAAGGATAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869838.1
CDS	258..9485
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKTSCLYNDPEMSSMEKDIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014349.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG 
TCTTCTATGG AGAAGGATAT TGACTTGAAG CTGAAAGAAA AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014350.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869839.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X22, mRNA.

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CloneID	OHu98477
Clone ID Related Accession (Same CDS sequence)	XM_017014350.1
Accession Version	XM_017014350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT 
AAGAGAAGTG TGAGTATTGA CTTGAAGCTG AAAGAAAAGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869839.1
CDS	258..9482
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVSIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014350.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA GATTCAGGAT ACTATGGGGA CGGTCTAAAT 
GCCATCATTG TGTTTGCCGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT 
GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGTTGATGGT AGCTGAAGAC 
TATATGATTG TGTACTTGAA TGGTGCAACC CCAAGAAGGA GGATGCCAGG GCTAGGCTGG 
ATGAAGAAAT GCTACCAGAT GATTGACAGA CGGTTGAGGA AGAATTTGAA ATCATTCATC 
ATTGTTCATC CATCTTGGTT CATCAGAACA ATCCTTGCTG TGACACGACC TTTTATAAGT 
TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC 
CCAATGGATT GCATCCACAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT 
AAGAGAAGTG TGAGTATTGA CTTGAAGCTG AAAGAAAAGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014351.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869840.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X25, mRNA.

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CloneID	OHu98478
Clone ID Related Accession (Same CDS sequence)	XM_017014351.1
Accession Version	XM_017014351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA 
AGTGTGAGTA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869840.1
CDS	258..9476
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVSIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014351.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014351.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGATACTATG GGGACGGTCT AAATGCCATC 
ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC AGCAGTCGGG CGGATTACCA CTATGTCATG 
GAAAATCTTT TCCTATATGT AATAAGTACT TTAGAGTTGA TGGTAGCTGA AGACTATATG 
ATTGTGTACT TGAATGGTGC AACCCCAAGA AGGAGGATGC CAGGGCTAGG CTGGATGAAG 
AAATGCTACC AGATGATTGA CAGACGGTTG AGGAAGAATT TGAAATCATT CATCATTGTT 
CATCCATCTT GGTTCATCAG AACAATCCTT GCTGTGACAC GACCTTTTAT AAGTTCAAAA 
TTCAGCAGTA AAATTAAATA TGTCAATAGC TTATCAGAAC TCAGTGGGCT GATCCCAATG 
GATTGCATCC ACATTCCAGA GAGCATCATC AAGTACGATG AAGAGAGATC TTATAAGAGA 
AGTGTGAGTA TTGACTTGAA GCTGAAAGAA AAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014352.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869841.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X26, mRNA.

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CloneID	OHu98479
Clone ID Related Accession (Same CDS sequence)	XM_017014352.1
Accession Version	XM_017014352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT 
GTGAGTATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869841.1
CDS	258..9473
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKYDEERSYKRSVSIDLKLKEKP

Target ORF information:

RefSeq Version	XM_017014352.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014352.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGATC CCACAGCCAA CAAAGATTCT 
GGCCAAGAGT CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG 
CTTTGGAGGA CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG 
CCCTACAGGA GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT 
GTGTTTGCCG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA 
AATCTTTTCC TATATGTAAT AAGTACTTTA GAGTTGATGG TAGCTGAAGA CTATATGATT 
GTGTACTTGA ATGGTGCAAC CCCAAGAAGG AGGATGCCAG GGCTAGGCTG GATGAAGAAA 
TGCTACCAGA TGATTGACAG ACGGTTGAGG AAGAATTTGA AATCATTCAT CATTGTTCAT 
CCATCTTGGT TCATCAGAAC AATCCTTGCT GTGACACGAC CTTTTATAAG TTCAAAATTC 
AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT 
TGCATCCACA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT 
GTGAGTATTG ACTTGAAGCT GAAAGAAAAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014353.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869842.1 Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X27, mRNA.

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CloneID	OHu98480
Clone ID Related Accession (Same CDS sequence)	XM_017014353.1 , XM_017014353.2
Accession Version	XM_017014353.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA 
GTAGGAAGTT ACTTATGCAC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869842.1
CDS	258..9464
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKCRGRNLVGSYLCTV

Target ORF information:

RefSeq Version	XM_017014353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 (Drosophila) (PRUNE2), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014353.1

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA 
GTAGGAAGTT ACTTATGCAC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRUNE2 ( XM_017014353.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869842.1 Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X27, mRNA.

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CloneID	OHu98480
Clone ID Related Accession (Same CDS sequence)	XM_017014353.1 , XM_017014353.2
Accession Version	XM_017014353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein prune homolog 2 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA 
GTAGGAAGTT ACTTATGCAC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869842.1
CDS	258..9464
Translation	MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHEDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGDSGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKCRGRNLVGSYLCTV

Target ORF information:

RefSeq Version	XM_017014353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014353.2

ORF Insert Sequence:

ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CTTGGAGAAG 
GTCCATGTGG TTATTGGGCC TAAATCGTGT GACTTGGATT CTCTCATTTC TACCTTCACA 
TATGCTTACT TTCTAGACAA GGTCAGTCCA CCAGGGGTTC TGTGTTTACC AGTGCTGAAC 
ATACCAAGAA CTGAATTCAA CTACTTCACC GAGACGAGGT TTATTTTAGA AGAGCTAAAT 
ATTTCCGAAT CATTCCACAT ATTCCGGGAT GAAATTAACC TGCATCAGCT AAATGATGAA 
GGGAAGTTAT CGATAACACT TGTTGGCAGC AGTGTGCTGG CGAGTGAAGA CAAAACTTTA 
GAATCAGCAG TTGTCAAAGT CATTAATCCG GTTGAGCAGA GCGATGCCAA CGTTGAGTTC 
CGAGAGTCTT CCTCTTCTCT CGTGCTAAAG GAGATTCTCC AAGAGGCTCC TGAGCTCATC 
ACCGAGCAAC TGGCTCATCG CCTCAGAGGT AGCATTCTTT TCAAGTGGAT GACCATGGAA 
TCAGAGAAGA TCTCAGAGAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCT 
AACTTGCCTC CAAGAGAGGA CATCATCAAC GTCCTACAGG AGACCCAGTT CAGTGCTCAG 
GGTTTAAGTA TTGAACAGAC AATGTTGAAA GATCTAAAGG AGCTGTCAGA TGGAGAAATA 
AAAGTGGCCA TTAGTACTGT GAGCATGAAC CTTGAGAATT GTCTATTTCA CAGCAATATT 
ACCAGTGACT TGAAAGCATT TACAGACAAG TTTGGTTTTG ATGTCCTCAT CCTGTTCTCC 
AGCTATCTGT CAGAGGAGCA GCAGCCGAGA CGACAGATTG CTGTGTACTC AGAAAACATG 
GAGCTGTGCA GTCAGATTTG CTGTGAGCTG GAAGAGTGTC AGAACCCTTG CCTAGAACTG 
GAGCCCTTTG ACTGTGGCTG TGATGAGATC CTGGTGTACC AACAAGAGGA CCCTTCAGTG 
ACTTGTGATC AGGTGGTTCT CGTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCAGAGATG 
GTCTCCAATA GCCGGACATC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC CCTCTCCCAG 
GGGTCTTCTG GGATTATGGA ATTGTATGGT TCTGACATAG AGCCACAACC CAGCTCTGTG 
AATTTCATAG AGAACCCTCC AGATCTCAAT GATTCTAACC AGGCTCAGGT GGATGCCAAT 
GTAGACCTTG TTAGCCCAGA CAGCGGACTG GCTACCATTA GGAGCAGCCG CTCATCCAAG 
GAGAGCTCTG TTTTCCTCAG TGACGACAGC CCCGTGGGAG AAGGTGCTGG GCCTCACCAC 
ACCCTTCTCC CAGGGCTTGA CTCCTACAGC CCCATCCCTG AAGGGGCGGT GGCGGAGGAA 
CATGCATGGT CTGGAGAACA CGGTGAGCAC TTCGACCTCT TCAATTTTGA CCCAGCACCC 
ATGGCTTCTG GGCAGTCCCA GCAATCTTCT CATTCTGCAG ACTACTCCCC AGCAGATGAC 
TTCTTCCCCA ACAGTGACCT GTCAGAAGGA CAGCTCCCCG CTGGGCCTGA AGGACTTGAT 
GGCATGGGAA CCAACATGTC TAATTATTCA TCCAGTTCAC TTTTGTCAGG GGCTGGCAAA 
GATAGCCTTG TGGAACATGA TGAGGAGTTT GTCCAGAGAC AAGACAGTCC CAGAGATAAC 
TCTGAAAGAA ATTTGAGCCT GACAGATTTT GTGGGAGATG AATCCCCTTC CCCAGAAAGG 
CTAAAAAATA CTGGAAAGAG GATCCCACCA ACACCCATGA ATAGTTTAGT AGAAAGCTCG 
CCATCCACTG AAGAACCAGC CTCACTCTAT ACAGAAGATA TGACCCAAAA AGCAACTGAC 
ACAGGTCACA TGGGGCCACC TCAGACCCAT GCACGGTGCA GCAGCTGGTG GGGTGGTTTG 
GAAATTGACT CCAAAAATAT TGCAGATGCG TGGAGTTCCA GTGAACAGGA ATCTGTTTTC 
CAGAGCCCTG AATCATGGAA AGAGCATAAG CCAAGCTCCA TTGATAGGAG AGCCTCAGAT 
TCTGTATTTC AACCAAAGAG CCTCGAATTT ACAAAGTCAG GTCCCTGGGA GTCTGAATTT 
GGTCAGCCTG AACTGGGTAG CAATGATATT CAAGACAAAA ATGAGGAAAG CTTGCCGTTC 
CAGAACCTGC CCATGGAGAA GTCACCTTTG CCAAATACAT CTCCCCAAGG AACAAACCAC 
CTGATAGAAG ACTTTGCTTC TTTGTGGCAT TCTGGTCGCT CTCCCACAGC CATGCCCGAG 
CCCTGGGGAA ATCCTACAGA TGATGGTGAA CCAGCAGCTG TGGCGCCATT CCCAGCCTGG 
AGTGCATTTG GTAAAGAAGA TCATGATGAA GCTTTAAAAA ATACCTGGAA TTTGCACCCA 
ACAAGCAGCA AGACACCTTC TGTTAGGGAC CCGAATGAGT GGGCCATGGC AAAAAGTGGG 
TTTGCCTTTT CTTCTTCAGA ACTACTGGAC AATTCACCCA GTGAGATAAA CAATGAAGCA 
GCTCCAGAAA TCTGGGGCAA GAAAAACAAT GACTCCAGGG ATCACATCTT TGCACCTGGA 
AATCCCAGTT CTGATCTGGA TCACACATGG ACTAATTCTA AGCCACCAAA AGAAGATCAG 
AATGGTTTAG TGGATCCTAA AACTAGGGGC AAGGTATATG AAAAGGTAGA TTCCTGGAAC 
CTTTTTGAGG AGAATATGAA GAAAGGAGGG TCAGATGTCC TAGTTCCTTG GGAAGATTCC 
TTCTTATCTT ACAAATGTTC TGATTACAGT GCATCCAACC TAGGAGAAGA TTCGGTGCCT 
TCCCCCTTAG ATACCAATTA TTCCACCTCA GACTCTTACA CATCACCAAC ATTTGCTGGA 
GACGAAAAGG AAACTGAACA CAAGCCATTT GCTAAAGAGG AAGGTTTTGA GTCAAAAGAT 
GGTAACTCCA CGGCAGAGGA GACTGACATT CCTCCTCAGT CACTGCAACA GTCATCTCGA 
AATCGAATCA GTTCAGGTCC TGGGAACCTA GACATGTGGG CTTCACCTCA TACAGATAAC 
AGTTCTGAAA TAAATACCAC TCACAACCTG GATGAAAATG AACTCAAGAC AGAGCACACA 
GATGGTAAGA ATATCTCCAT GGAGGATGAC GTCGGGGAAA GCAGCCAGTC CAGTTACGAC 
GACCCCAGCA TGATGCAACT GTACAATGAA ACAAACCGAC AACTCACACT TTTGCACAGC 
AGCACCAACT CCCGGCAGAC GGCCCCTGAC AGTCTCGACT TGTGGAACAG AGTGATTTTG 
GAGGATACTC AGTCCACTGC AACGATCTCA GATATGGACA ATGATTTGGA CTGGGATGAC 
TGCAGTGGGG GTGCGGCAAT CCCCAGTGAT GGTCAAACAG AAGGATACAT GGCTGAAGGT 
TCCGAGCCGG AAACCCGATT TACAGTGAGA CAGCTGGAAC CCTGGGGCTT GGAGTATCAG 
GAAGCAAATC AGGTAGATTG GGAGCTCCCT GCCTCTGATG AGCATACCAA GGACAGTGCT 
CCCAGTGAAC ATCACACATT GAATGAGAAA AGTGGGCAGC TAATTGCAAA CAGTATTTGG 
GATTCTGTCA TGAGAGATAA AGACATGTCA TCATTCATGT TACCAGGCTC CTCACATATC 
ACAGATTCAG AGCAAAGGGA ATTGCCTCCT GAAATCCCCA GCCATTCAGC AAATGTTAAA 
GACACTCATT CCCCAGATGC GCCAGCAGCC TCTGGAACCA GTGAATCAGA GGCACTTATA 
TCTCATCTTG ACAAGCAGGA CACAGAGAGG GAAACCCTGC AAAGTGATGC AGCATCCTTG 
GCGACTAGGC TTGAGAATCC AGGGTATTTT CCACACCCAG ATCCATGGAA AGGTCATGGC 
GATGGACAAA GTGAAAGTGA GAAGGAAGCC CAGGGAGCCA CTGACAGGGG GCACCTTGAT 
GAAGAGGAGG TGATCGCCTC TGGTGTGGAG AATGCCTCAG GGATTTCTGA AAAAGGGCAG 
AGTGACCAGG AACTGTCTTC TCTGGTTGCA TCTGAACATC AGGAAATCTG CATTAAATCA 
GGCAAAATCA GCTCTCTTGC TGTCACTTTC AGTCCTCAAA CCGAGGAACC AGAGGAAGTT 
TTAGAGTATG AGGAGGGGTC TTACAATCTA GACTCCCGTG ATGTGCAAAC AGGGATGTCC 
GCAGATAACC TGCAGCCAAA AGATACCCAT GAAAAACACC TCATGAGTCA AAGAAATTCA 
GGTGAAACTA CTGAGACTTC AGATGGGATG AATTTCACAA AATATGTATC TGTACCTGAA 
AAGGATCTTG AGAAAACTGA AGAATGTAAC TTTCTAGAGC CAGAGAACGT GGGTGGAGGG 
CCACCTCACA GAGTTCCCCG AAGTCTTGAT TTTGGGGACG TCCCTATAGA CAGTGATGTG 
CATGTCAGCA GCACATGTTC TGAGATAACC AAAAATCTTG ACGTTAAGGG GTCTGAAAAT 
AGCCTTCCAG GAGCCGGTTC GTCTGGAAAT TTTGACAGAG ATACTATTTC TAGTGAGTAT 
ACTCATTCAA GTGCATCAAG TCCTGAGTTA AATGACTCTT CAGTTGCACT GTCCTCCTGG 
GGCCAGCAAC CCAGTTCTGG GTATCAAGAA GAAAACCAAG GCAACTGGAG TGAACAGAAT 
CACCAAGAAT CTGAACTAAT TACCACTGAT GGCCAAGTAG AAATAGTTAC CAAAGTGAAG 
GATTTAGAGA AAAACAGAAT AAATGAGTTT GAAAAGAGCT TTGATCGCAA AACTCCTACA 
TTTTTAGAGA TCTGGAATGA CTCAGTTGAT GGTGATTCCT TTTCCTCTTT ATCCAGTCCT 
GAAACAGGCA AATATTCTGA ACATTCAGGG ACACATCAGG AAAGCAATCT AATTGCTAGC 
TACCAGGAGA AAAATGAACA TGACATTTCT GCAACTGTGC AGCCAGAGGA TGCCAGGGTC 
ATTTCAACAA GCTCAGGTTC TGATGATGAC AGTGTCGGTG GTGAAGAGTC AATAGAGGAA 
GAGATCCAGG TGGCCAACTG CCACGTTGCT GAGGATGAAT CCAGAGCTTG GGATTCATTG 
AATGAATCTA ATAAGTTCTT GGTCACAGCT GATCCTAAGT CTGAAAATAT TTATGACTAC 
CTAGACAGCT CAGAGCCAGC AGAGAATGAG AATAAGTCAA ACCCATTCTG TGACAATCAA 
CAAAGCAGCC CTGATCCCTG GACTTTCTCA CCATTAACGG AGACTGAAAT GCAGATTACA 
GCAGTGGAGA AGGAGAAGAG ATCTTCTCCA GAAACAGGGA CAACAGGAGA TGTTGCATGG 
CAAATATCTC CCAAAGCTTC GTTCCCAAAG AACGAAGATA ATTCTCAACT GGAAATGCTG 
GGCTTCTCAG CTGATAGCAC TGAGTGGTGG AAGGCCTCAC CCCAGGAAGG GAGACTAATT 
GAAAGTCCAT TTGAAAGGGA GCTGTCTGAC TCCAGTGGTG TGTTGGAGAT AAATTCTTCA 
GTACACCAAA ATGCCAGTCC CTGGGGAGTA CCAGTTCAGG GTGATATTGA GCCCGTGGAA 
ACACACTATA CTAATCCTTT TAGTGACAAC CATCAGTCAC CCTTTCTGGA AGGTAATGGG 
AAGAACTCCC ATGAGCAACT CTGGAACATT CAACCAAGGC AGCCAGACCC AGATGCTGAC 
AAGTTCAGCC AGCTTGTAAA ATTAGACCAA ATTAAAGAAA AGGACTCAAG AGAGCAAACC 
TTTGTGTCTG CTGCTGGTGA TGAGCTGACT CCTGAAACAC CTACCCAAGA GCAGTGTCAG 
GACACCATGC TGCCAGTCTG TGATCATCCG GACACAGCCT TTACTCACGC AGAGGAAAAT 
AGTTGTGTTA CATCTAATGT TTCAACTAAT GAAGGTCAAG AAACAAATCA GTGGGAACAA 
GAAAAATCAT ACCTAGGTGA GATGACAAAT TCAAGCATTG CCACAGAAAA TTTTCCTGCT 
GTCAGTTCTC CCACCCAACT GATAATGAAG CCAGGCTCTG AATGGGATGG CTCTACCCCA 
AGTGAGGACT CCCGAGGTAC CTTTGTGCCA GATATTTTAC ATGGCAACTT TCAAGAGGGT 
GGGCAGCTGG CCTCTGCCGC GCCTGACTTG TGGATAGATG CTAAGAAGCC CTTCAGTTTG 
AAAGCAGATG GTGAGAATCC TGATATCCTG ACGCACTGCG AACATGACAG CAATTCTCAG 
GCTTCCGACA GCCCTGATAT ATGTCACGAT TCTGAAGCAA AGCAAGAGAC TGAGAAGCAC 
CTCAGTGCTT GCATGGGACC TGAAGTGGAA TCCAGTGAGC TTTGTCTCAC TGAGCCAGAG 
ATAGATGAAG AACCCATTTA TGAGCCTGGA CGGGAGTTTG TCCCATCCAA TGCAGAACTC 
GATTCTGAAA ACGCAACTGT GCTGCCTCCA ATTGGCTATC AAGCAGACAT AAAGGGCTCC 
TCTCAGCCCG CCTCTCATAA AGGTTCACCT GAACCTTCTG AAATAAACGG TGACAACAGT 
ACAGGTTTAC AAGTATCAGA AAAAGGAGCC AGCCCAGATA TGGCACCAAT TTTGGAACCA 
GTTGACAGAA GAATCCCAAG GATTGAAAAT GTGGCAACTA GCATTTTTGT AACTCACCAA 
GAGCCAACTC CAGAAGGTGA CGGTTCTTGG ATATCAGACA GCTTTTCTCC TGAAAGTCAG 
CCTGGTGCAA GAGCTTTGTT TGATGGTGAT CCACATTTAT CCACAGAGAA TCCTGCCTTG 
GTTCCTGATG CTTTGCTAGC CTCAGACACT TGTCTGGATA TAAGCGAAGC TGCCTTTGAC 
CACAGTTTCA GCGATGCCTC AGGTCTCAAC ACATCCACGG GAACAATAGA TGACATGAGT 
AAACTGACAT TATCCGAAGG CCATCCGGAA ACGCCAGTTG ATGGGGACCT AGGGAAGCAA 
GATATCTGCT CATCTGAAGC CTCGTGGGGT GATTTTGAAT ATGATGTAAT GGGCCAGAAT 
ATCGATGAAG ATTTACTGAG AGAGCCTGAA CACTTCCTGT ATGGTGGTGA CCCTCCTTTG 
GAGGAAGATT CTCTGAAGCA GTCGCTGGCA CCGTACACAC CTCCCTTTGA TTTGTCTTAT 
CTCACAGAAC CTGCCCAGAG TGCTGAAACA ATAGAGGAAG CTGGGTCTCC AGAGGATGAA 
TCTCTGGGAT GCAGAGCAGC AGAGATAGTG CTTTCTGCAC TTCCTGATCG AAGAAGTGAG 
GGAAACCAGG CTGAGACCAA AAACAGACTG CCTGGATCCC AGCTGGCTGT GCTGCATATT 
CGTGAAGACC CTGAGTCCGT TTATTTGCCG GTAGGAGCAG GCTCCAACAT TTTGTCTCCA 
TCAAACGTTG ACTGGGAAGT AGAAACAGAT AATTCTGATT TACCAGCAGG TGGAGACATA 
GGACCACCAA ATGGTGCCAG CAAGGAAATA TCAGAATTGG AAGAAGAAAA AACAATTCCT 
ACCAAAGAGC CTGAGCAGAT AAAATCAGAA TACAAGGAAG AAAGATGTAC AGAGAAGAAT 
GAAGATCGTC ATGCACTACA CATGGATTAC ATACTTGTAA ACCGTGAAGA AAATTCACAC 
TCAAAGCCAG AGACCTGTGA AGAAAGAGAA AGCATAGCTG AATTAGAATT GTATGTAGGT 
TCCAAAGAAA CAGGGCTGCA GGGAACTCAG TTAGCAAGCT TCCCAGACAC ATGTCAGCCA 
GCCTCCTTAA ATGAAAGAAA AGGTCTCTCT GCAGAGAAAA TGTCTTCTAA AAGCGATACG 
AGATCATCTT TTGAAAGCCC TGCACAAGAC CAGAGTTGGA TGTTCTTGGG CCATAGTGAG 
GTTGGTGATC CATCACTGGA TGCCAGGGAC TCAGGGCCTG GGTGGTCTGG CAAGACTGTG 
GAGCCGTTCT CTGAACTCGG CTTGGGTGAG GGTCCCCAGC TGCAGATTCT GGAAGAAATG 
AAGCCTCTAG AATCTTTGGC ACTAGAGGAA GCCTCTGGTC CAGTCAGCCA ATCACAGAAG 
AGTAAGAGCC GAGGCAGGGC TGGCCCGGAT GCAGTTACGT TGCAGGCTGT CACCCATGAC 
AATGAATGGG AAATGCTTTC ACCACAGCCT GTTCAGAAAA ACATGATCCC TGACACGGAA 
ATGGAGGAGG AGACAGAGTT CCTTGAGCTC GGAACCAGGA TATCAAGACC AAATGGACTA 
CTGTCAGAGG ATGTAGGAAT GGACATCCCC TTTGAAGAGG GCGTGCTGAG TCCCAGTGCT 
GCAGACATGA GGCCTGAACC TCCTAATTCT CTGGATCTTA ATGACACTCA TCCTCGGAGA 
ATCAAGCTCA CAGCCCCAAA TATCAATCTT TCTCTGGACC AAAGTGAAGG ATCTATTCTC 
TCTGATGATA ACTTGGACAG TCCAGATGAA ATTGACATCA ATGTGGATGA ACTTGATACC 
CCCGATGAAG CAGATTCTTT TGAGTACACT GGCCATGAAG ATCCCACAGC CAACAAAGAT 
TCTGGCCAAG AGTCAGAGTC TATTCCAGAA TATACGGCCG AAGAGGAACG GGAGGACAAC 
CGGCTTTGGA GGACAGTGGT CATTGGAGAA CAAGAGCAGC GCATTGACAT GAAGGTCATC 
GAGCCCTACA GGAGAGTCAT TTCTCACGGA GGAGATTCAG GATACTATGG GGACGGTCTA 
AATGCCATCA TTGTGTTTGC CGCCTGTTTT CTGCCAGACA GCAGTCGGGC GGATTACCAC 
TATGTCATGG AAAATCTTTT CCTATATGTA ATAAGTACTT TAGAGTTGAT GGTAGCTGAA 
GACTATATGA TTGTGTACTT GAATGGTGCA ACCCCAAGAA GGAGGATGCC AGGGCTAGGC 
TGGATGAAGA AATGCTACCA GATGATTGAC AGACGGTTGA GGAAGAATTT GAAATCATTC 
ATCATTGTTC ATCCATCTTG GTTCATCAGA ACAATCCTTG CTGTGACACG ACCTTTTATA 
AGTTCAAAAT TCAGCAGTAA AATTAAATAT GTCAATAGCT TATCAGAACT CAGTGGGCTG 
ATCCCAATGG ATTGCATCCA CATTCCAGAG AGCATCATCA AATGCAGGGG ACGTAACTTA 
GTAGGAAGTT ACTTATGCAC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional regulation of BMCC1 mediated by E2F1 in neuroblastoma cells.
Biochemical and biophysical research communications478(1)81-86(2016 09)
Islam MS,Tatsumi Y,Takano R,Yokochi T,Akter J,Ozaki T,Nakamura Y,Ohira M,Nakagawara A

PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3.
Proceedings of the National Academy of Sciences of the United States of America112(27)8403-8(2015 Jul)
Salameh A,Lee AK,Card?-Vila M,Nunes DN,Efstathiou E,Staquicini FI,Dobroff AS,Marchi? S,Navone NM,Hosoya H,Lauer RC,Wen S,Salmeron CC,Hoang A,Newsham I,Lima LA,Carraro DM,Oliviero S,Kolonin MG,Sidman RL,Do KA,Troncoso P,Logothetis CJ,Brentani RR,Calin GA,Cavenee WK,Dias-Neto E,Pasqualini R,Arap W

Identification and diagnostic performance of a small RNA within the PCA3 and BMCC1 gene locus that potentially targets mRNA.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology24(1)268-75(2015 Jan)
Drayton RM,Rehman I,Clarke R,Zhao Z,Pang K,Miah S,Stoehr R,Hartmann A,Blizard S,Lavin M,Bryant HE,Martens-Uzunova ES,Jenster G,Hamdy FC,Gardiner RA,Catto JW

BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.
Cell death & disease6e1607(2015 Jan)
Tatsumi Y,Takano R,Islam MS,Yokochi T,Itami M,Nakamura Y,Nakagawara A

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PRUNE2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: