BEST3 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001282615.1	NP_001269544.1	bestrophin-3 isoform 5
XM_011537961.1	XP_011536263.1	bestrophin-3 isoform X2
XM_011537963.1	XP_011536265.1	bestrophin-3 isoform X3
NM_032735.2	NP_116124.2	bestrophin-3 isoform 1
XM_006719256.3	XP_006719319.1	bestrophin-3 isoform X3
NM_001282615.2	NP_001269544.1	bestrophin-3 isoform 5
NM_032735.3	NP_116124.2	bestrophin-3 isoform 1
NM_001282613.1	NP_001269542.1	bestrophin-3 isoform 3
NM_152439.3	NP_689652.2	bestrophin-3 isoform 2
XM_011537962.2	XP_011536264.1	bestrophin-3 isoform X3
NM_001282616.2	NP_001269545.1	bestrophin-3 isoform 5
XM_011537965.2	XP_011536267.1	bestrophin-3 isoform X4
NM_001282616.1	NP_001269545.1	bestrophin-3 isoform 5
XM_017018865.1	XP_016874354.1	bestrophin-3 isoform X5
NM_001282614.1	NP_001269543.1	bestrophin-3 isoform 4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05038	NM_001282613.1 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu05084	NM_032735.3 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$334.50-$468.30 $669.00
OHu08218	NM_001282616.2 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08218	NM_001282615.2 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05038	XM_011537961.1 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu46341	XM_011537963.1 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu46341	XM_011537962.2 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu76091	XM_011537965.2 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu00668	NM_001282614.1 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu06779	NM_152439.3 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu46342	XM_006719256.3 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu83546	XM_017018865.1 Latest version!	Homo sapiens bestrophin 3 (BEST3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu08218	NM_001282615.1	Homo sapiens bestrophin 3 (BEST3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05084	NM_032735.2	Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$334.50-$468.30 $669.00
OHu08218	NM_001282616.1	Homo sapiens bestrophin 3 (BEST3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

BEST3 ( NM_001282613.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269542.1 Homo sapiens bestrophin 3 (BEST3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05038
Clone ID Related Accession (Same CDS sequence)	XM_011537961.1 , NM_001282613.1
Accession Version	NM_001282613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA767581.1, BC151138.1 and BC028087.1. On Sep 14, 2013 this sequence version replaced XM_005268672.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter at the N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.128975.1, SRR1803613.248916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGGTTTTAT GACAACAGAT 
GAAAGGAAAT TATTCAACCA CCTCAAGTCT CCTCATCTGA AATATTGGGT TCCATTCATC 
TGGTTTGGAA ATCTTGCAAC TAAAGCCCGG AATGAAGGTA GAATCAGAGA CAGTGTTGAT 
CTGCAATCAT TGATGACTGA AATGAATCGA TACCGCTCTT GGTGCAGCCT CTTATTCGGT 
TATGACTGGG TTGGGATTCC GCTGGTTTAC ACCCAGGTTG TCACTCTTGC TGTCTATACC 
TTCTTCTTTG CGTGCCTGAT TGGACGCCAG TTTTTGGATC CCACCAAAGG CTACGCAGGG 
CATGACTTGG ATCTTTACAT TCCCATCTTC ACCCTCCTAC AATTCTTCTT CTATGCAGGA 
TGGCTTAAGG TAGCAGAGCA GCTTATCAAC CCTTTTGGAG AAGATGATGA TGATTTTGAA 
ACTAACTGGT GCATTGACAG AAATTTGCAG GTCTCTCTTT TAGCTGTGGA CGAAATGCAC 
ATGAGCTTAC CCAAGATGAA GAAGGACATT TACTGGGACG ATTCTGCTGC TCGCCCACCA 
TACACATTGG CAGCTGCTGA CTACTGCATA CCCTCATTTC TGGGGTCAAC AGTCCAGATG 
GGGCTGTCTG GGTCCGACTT TCCTGACGAG GAGTGGCTGT GGGATTATGA GAAGCATGGC 
CATCGGCATT CCATGATAAG AAGAGTCAAG CGGTTCCTGA GTGCCCACGA ACACCCCTCC 
AGCCCCAGAA GAAGAAGCTA CAGGAGGCAG ACAAGTGACA GCTCCATGTT CTTACCCCGA 
GATGACCTCA GCCCAGCCAG GGACCTACTG GATGTGCCCT CAAGAAACCC CCCCAGGGCC 
TCACCCACCT GGAAGAAATC CTGCTTCCCA GAAGGAAGCC CCACGCTGCA CTTCAGCATG 
GGAGAGCTGT CCACCATCAG GGAGACCAGC CAGACAAGCA CTTTACAGAG CCTGACCCCA 
CAGTCCAGTG TGAGAACTTC CCCCATCAAA ATGCCACTGG TACCTGAGGT ATTGATCACA 
GCAGCCGAAG CACCAGTGCC CACATCAGGG GGCTACCACC ATGATTCCGC TACCTCCATC 
TTGAGCTCTG AGTTTACAGG GGTTCAGCCA AGCAAGACTG AGCAGCAGCA GGGCCCCATG 
GGATCCATCC TGTCTCCCTC AGAGAAGGAG ACACCTCCTG GAGGCCCCAG TCCCCAGACA 
GTTTCAGCCA GCGCTGAGGA AAATATATTC AACTGTGAAG AAGACCCTGG TGATACCTTT 
CTAAAAAGGT GGAGTCTTCC GGGATTCCTG GGGTCCAGCC ACACTTCCCT GGGAAACCTA 
AGTCCAGACC CCATGAGCTC TCAGCCAGCT CTTTTAATTG ACACAGAAAC ATCCTCAGAG 
ATCAGTGGGA TCAACATTGT GGCTGGCTCT CGAGTCTCTT CTGATATGCT GTATTTAATG 
GAAAACCTGG ACACCAAGGA AACAGATATC ATAGAGCTGA ACAAGGAAAC TGAGGAATCA 
CCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269542.1
CDS	284..1972
Translation	MFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	NM_001282613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282613.1

ORF Insert Sequence:

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGGTTTTAT GACAACAGAT 
GAAAGGAAAT TATTCAACCA CCTCAAGTCT CCTCATCTGA AATATTGGGT TCCATTCATC 
TGGTTTGGAA ATCTTGCAAC TAAAGCCCGG AATGAAGGTA GAATCAGAGA CAGTGTTGAT 
CTGCAATCAT TGATGACTGA AATGAATCGA TACCGCTCTT GGTGCAGCCT CTTATTCGGT 
TATGACTGGG TTGGGATTCC GCTGGTTTAC ACCCAGGTTG TCACTCTTGC TGTCTATACC 
TTCTTCTTTG CGTGCCTGAT TGGACGCCAG TTTTTGGATC CCACCAAAGG CTACGCAGGG 
CATGACTTGG ATCTTTACAT TCCCATCTTC ACCCTCCTAC AATTCTTCTT CTATGCAGGA 
TGGCTTAAGG TAGCAGAGCA GCTTATCAAC CCTTTTGGAG AAGATGATGA TGATTTTGAA 
ACTAACTGGT GCATTGACAG AAATTTGCAG GTCTCTCTTT TAGCTGTGGA CGAAATGCAC 
ATGAGCTTAC CCAAGATGAA GAAGGACATT TACTGGGACG ATTCTGCTGC TCGCCCACCA 
TACACATTGG CAGCTGCTGA CTACTGCATA CCCTCATTTC TGGGGTCAAC AGTCCAGATG 
GGGCTGTCTG GGTCCGACTT TCCTGACGAG GAGTGGCTGT GGGATTATGA GAAGCATGGC 
CATCGGCATT CCATGATAAG AAGAGTCAAG CGGTTCCTGA GTGCCCACGA ACACCCCTCC 
AGCCCCAGAA GAAGAAGCTA CAGGAGGCAG ACAAGTGACA GCTCCATGTT CTTACCCCGA 
GATGACCTCA GCCCAGCCAG GGACCTACTG GATGTGCCCT CAAGAAACCC CCCCAGGGCC 
TCACCCACCT GGAAGAAATC CTGCTTCCCA GAAGGAAGCC CCACGCTGCA CTTCAGCATG 
GGAGAGCTGT CCACCATCAG GGAGACCAGC CAGACAAGCA CTTTACAGAG CCTGACCCCA 
CAGTCCAGTG TGAGAACTTC CCCCATCAAA ATGCCACTGG TACCTGAGGT ATTGATCACA 
GCAGCCGAAG CACCAGTGCC CACATCAGGG GGCTACCACC ATGATTCCGC TACCTCCATC 
TTGAGCTCTG AGTTTACAGG GGTTCAGCCA AGCAAGACTG AGCAGCAGCA GGGCCCCATG 
GGATCCATCC TGTCTCCCTC AGAGAAGGAG ACACCTCCTG GAGGCCCCAG TCCCCAGACA 
GTTTCAGCCA GCGCTGAGGA AAATATATTC AACTGTGAAG AAGACCCTGG TGATACCTTT 
CTAAAAAGGT GGAGTCTTCC GGGATTCCTG GGGTCCAGCC ACACTTCCCT GGGAAACCTA 
AGTCCAGACC CCATGAGCTC TCAGCCAGCT CTTTTAATTG ACACAGAAAC ATCCTCAGAG 
ATCAGTGGGA TCAACATTGT GGCTGGCTCT CGAGTCTCTT CTGATATGCT GTATTTAATG 
GAAAACCTGG ACACCAAGGA AACAGATATC ATAGAGCTGA ACAAGGAAAC TGAGGAATCA 
CCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_032735.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_116124.2 Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05084
Clone ID Related Accession (Same CDS sequence)	NM_032735.2 , NM_032735.3
Accession Version	NM_032735.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK096459.1, AC025263.22 and BC028087.1. On or before Aug 31, 2013 this sequence version replaced XM_005268671.1, NM_032735.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## CDS exon combination :: AK096459.1, AY515706.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GCTGTCTGGG TCCGACTTTC CTGACGAGGA GTGGCTGTGG 
GATTATGAGA AGCATGGCCA TCGGCATTCC ATGATAAGAA GAGTCAAGCG GTTCCTGAGT 
GCCCACGAAC ACCCCTCCAG CCCCAGAAGA AGAAGCTACA GGAGGCAGAC AAGTGACAGC 
TCCATGTTCT TACCCCGAGA TGACCTCAGC CCAGCCAGGG ACCTACTGGA TGTGCCCTCA 
AGAAACCCCC CCAGGGCCTC ACCCACCTGG AAGAAATCCT GCTTCCCAGA AGGAAGCCCC 
ACGCTGCACT TCAGCATGGG AGAGCTGTCC ACCATCAGGG AGACCAGCCA GACAAGCACT 
TTACAGAGCC TGACCCCACA GTCCAGTGTG AGAACTTCCC CCATCAAAAT GCCACTGGTA 
CCTGAGGTAT TGATCACAGC AGCCGAAGCA CCAGTGCCCA CATCAGGGGG CTACCACCAT 
GATTCCGCTA CCTCCATCTT GAGCTCTGAG TTTACAGGGG TTCAGCCAAG CAAGACTGAG 
CAGCAGCAGG GCCCCATGGG ATCCATCCTG TCTCCCTCAG AGAAGGAGAC ACCTCCTGGA 
GGCCCCAGTC CCCAGACAGT TTCAGCCAGC GCTGAGGAAA ATATATTCAA CTGTGAAGAA 
GACCCTGGTG ATACCTTTCT AAAAAGGTGG AGTCTTCCGG GATTCCTGGG GTCCAGCCAC 
ACTTCCCTGG GAAACCTAAG TCCAGACCCC ATGAGCTCTC AGCCAGCTCT TTTAATTGAC 
ACAGAAACAT CCTCAGAGAT CAGTGGGATC AACATTGTGG CTGGCTCTCG AGTCTCTTCT 
GATATGCTGT ATTTAATGGA AAACCTGGAC ACCAAGGAAA CAGATATCAT AGAGCTGAAC 
AAGGAAACTG AGGAATCACC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116124.2
CDS	228..2234
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	NM_032735.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032735.2

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GCTGTCTGGG TCCGACTTTC CTGACGAGGA GTGGCTGTGG 
GATTATGAGA AGCATGGCCA TCGGCATTCC ATGATAAGAA GAGTCAAGCG GTTCCTGAGT 
GCCCACGAAC ACCCCTCCAG CCCCAGAAGA AGAAGCTACA GGAGGCAGAC AAGTGACAGC 
TCCATGTTCT TACCCCGAGA TGACCTCAGC CCAGCCAGGG ACCTACTGGA TGTGCCCTCA 
AGAAACCCCC CCAGGGCCTC ACCCACCTGG AAGAAATCCT GCTTCCCAGA AGGAAGCCCC 
ACGCTGCACT TCAGCATGGG AGAGCTGTCC ACCATCAGGG AGACCAGCCA GACAAGCACT 
TTACAGAGCC TGACCCCACA GTCCAGTGTG AGAACTTCCC CCATCAAAAT GCCACTGGTA 
CCTGAGGTAT TGATCACAGC AGCCGAAGCA CCAGTGCCCA CATCAGGGGG CTACCACCAT 
GATTCCGCTA CCTCCATCTT GAGCTCTGAG TTTACAGGGG TTCAGCCAAG CAAGACTGAG 
CAGCAGCAGG GCCCCATGGG ATCCATCCTG TCTCCCTCAG AGAAGGAGAC ACCTCCTGGA 
GGCCCCAGTC CCCAGACAGT TTCAGCCAGC GCTGAGGAAA ATATATTCAA CTGTGAAGAA 
GACCCTGGTG ATACCTTTCT AAAAAGGTGG AGTCTTCCGG GATTCCTGGG GTCCAGCCAC 
ACTTCCCTGG GAAACCTAAG TCCAGACCCC ATGAGCTCTC AGCCAGCTCT TTTAATTGAC 
ACAGAAACAT CCTCAGAGAT CAGTGGGATC AACATTGTGG CTGGCTCTCG AGTCTCTTCT 
GATATGCTGT ATTTAATGGA AAACCTGGAC ACCAAGGAAA CAGATATCAT AGAGCTGAAC 
AAGGAAACTG AGGAATCACC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_032735.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116124.2 Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05084
Clone ID Related Accession (Same CDS sequence)	NM_032735.2 , NM_032735.3
Accession Version	NM_032735.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK096459.1, AC025263.22 and BC028087.1. On Nov 23, 2018 this sequence version replaced NM_032735.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## CDS exon combination :: AK096459.1, AY515706.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000330891.10/ ENSP00000332413.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GCTGTCTGGG TCCGACTTTC CTGACGAGGA GTGGCTGTGG 
GATTATGAGA AGCATGGCCA TCGGCATTCC ATGATAAGAA GAGTCAAGCG GTTCCTGAGT 
GCCCACGAAC ACCCCTCCAG CCCCAGAAGA AGAAGCTACA GGAGGCAGAC AAGTGACAGC 
TCCATGTTCT TACCCCGAGA TGACCTCAGC CCAGCCAGGG ACCTACTGGA TGTGCCCTCA 
AGAAACCCCC CCAGGGCCTC ACCCACCTGG AAGAAATCCT GCTTCCCAGA AGGAAGCCCC 
ACGCTGCACT TCAGCATGGG AGAGCTGTCC ACCATCAGGG AGACCAGCCA GACAAGCACT 
TTACAGAGCC TGACCCCACA GTCCAGTGTG AGAACTTCCC CCATCAAAAT GCCACTGGTA 
CCTGAGGTAT TGATCACAGC AGCCGAAGCA CCAGTGCCCA CATCAGGGGG CTACCACCAT 
GATTCCGCTA CCTCCATCTT GAGCTCTGAG TTTACAGGGG TTCAGCCAAG CAAGACTGAG 
CAGCAGCAGG GCCCCATGGG ATCCATCCTG TCTCCCTCAG AGAAGGAGAC ACCTCCTGGA 
GGCCCCAGTC CCCAGACAGT TTCAGCCAGC GCTGAGGAAA ATATATTCAA CTGTGAAGAA 
GACCCTGGTG ATACCTTTCT AAAAAGGTGG AGTCTTCCGG GATTCCTGGG GTCCAGCCAC 
ACTTCCCTGG GAAACCTAAG TCCAGACCCC ATGAGCTCTC AGCCAGCTCT TTTAATTGAC 
ACAGAAACAT CCTCAGAGAT CAGTGGGATC AACATTGTGG CTGGCTCTCG AGTCTCTTCT 
GATATGCTGT ATTTAATGGA AAACCTGGAC ACCAAGGAAA CAGATATCAT AGAGCTGAAC 
AAGGAAACTG AGGAATCACC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116124.2
CDS	115..2121
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	NM_032735.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032735.3

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GCTGTCTGGG TCCGACTTTC CTGACGAGGA GTGGCTGTGG 
GATTATGAGA AGCATGGCCA TCGGCATTCC ATGATAAGAA GAGTCAAGCG GTTCCTGAGT 
GCCCACGAAC ACCCCTCCAG CCCCAGAAGA AGAAGCTACA GGAGGCAGAC AAGTGACAGC 
TCCATGTTCT TACCCCGAGA TGACCTCAGC CCAGCCAGGG ACCTACTGGA TGTGCCCTCA 
AGAAACCCCC CCAGGGCCTC ACCCACCTGG AAGAAATCCT GCTTCCCAGA AGGAAGCCCC 
ACGCTGCACT TCAGCATGGG AGAGCTGTCC ACCATCAGGG AGACCAGCCA GACAAGCACT 
TTACAGAGCC TGACCCCACA GTCCAGTGTG AGAACTTCCC CCATCAAAAT GCCACTGGTA 
CCTGAGGTAT TGATCACAGC AGCCGAAGCA CCAGTGCCCA CATCAGGGGG CTACCACCAT 
GATTCCGCTA CCTCCATCTT GAGCTCTGAG TTTACAGGGG TTCAGCCAAG CAAGACTGAG 
CAGCAGCAGG GCCCCATGGG ATCCATCCTG TCTCCCTCAG AGAAGGAGAC ACCTCCTGGA 
GGCCCCAGTC CCCAGACAGT TTCAGCCAGC GCTGAGGAAA ATATATTCAA CTGTGAAGAA 
GACCCTGGTG ATACCTTTCT AAAAAGGTGG AGTCTTCCGG GATTCCTGGG GTCCAGCCAC 
ACTTCCCTGG GAAACCTAAG TCCAGACCCC ATGAGCTCTC AGCCAGCTCT TTTAATTGAC 
ACAGAAACAT CCTCAGAGAT CAGTGGGATC AACATTGTGG CTGGCTCTCG AGTCTCTTCT 
GATATGCTGT ATTTAATGGA AAACCTGGAC ACCAAGGAAA CAGATATCAT AGAGCTGAAC 
AAGGAAACTG AGGAATCACC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_001282615.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269544.1 Homo sapiens bestrophin 3 (BEST3), transcript variant 5, mRNA.

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CloneID	OHu08218
Clone ID Related Accession (Same CDS sequence)	NM_001282615.1 , NM_001282615.2 , NM_001282616.2 , NM_001282616.1
Accession Version	NM_001282615.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA767581.1, BC006440.1 and AC025263.22. Transcript Variant: This variant (5) lacks a portion of the 5' UTR and 5' coding region, uses a downstream in-frame start codon, and lacks several 3' exons but includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (5) is shorter at the N-terminus, has a distinct C-terminus and is significantly shorter than isoform 1. Both variants 5 and 6 encode isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC006440.1, BG819513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269544.1
CDS	284..502
Translation	MFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAERTGMKPILPSSFEMQSF

Target ORF information:

RefSeq Version	NM_001282615.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282615.1

ORF Insert Sequence:

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_001282615.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269544.1 Homo sapiens bestrophin 3 (BEST3), transcript variant 5, mRNA.

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CloneID	OHu08218
Clone ID Related Accession (Same CDS sequence)	NM_001282615.1 , NM_001282615.2 , NM_001282616.2 , NM_001282616.1
Accession Version	NM_001282615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC006440.1 and AC025263.22. On May 31, 2019 this sequence version replaced NM_001282615.1. Transcript Variant: This variant (5) lacks a portion of the 5' UTR and 5' coding region, uses a downstream in-frame start codon, and lacks several 3' exons but includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (5) is shorter at the N-terminus, has a distinct C-terminus and is significantly shorter than isoform 1. Both variants 5 and 6 encode isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC006440.1, BI225734.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269544.1
CDS	171..389
Translation	MFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAERTGMKPILPSSFEMQSF

Target ORF information:

RefSeq Version	NM_001282615.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282615.2

ORF Insert Sequence:

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_001282616.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269545.1 Homo sapiens bestrophin 3 (BEST3), transcript variant 6, mRNA.

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CloneID	OHu08218
Clone ID Related Accession (Same CDS sequence)	NM_001282615.1 , NM_001282615.2 , NM_001282616.2 , NM_001282616.1
Accession Version	NM_001282616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP225850.1, AC025263.22 and BC006440.1. On May 31, 2019 this sequence version replaced NM_001282616.1. Transcript Variant: This variant (6) lacks a portion of the 5' UTR and 5' coding region, uses a downstream in-frame start codon, and lacks several 3' exons but includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (5) is shorter at the N-terminus, has a distinct C-terminus and is significantly shorter than isoform 1. Both variants 5 and 6 encode isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BP225850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269545.1
CDS	266..484
Translation	MFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAERTGMKPILPSSFEMQSF

Target ORF information:

RefSeq Version	NM_001282616.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282616.2

ORF Insert Sequence:

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_001282616.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269545.1 Homo sapiens bestrophin 3 (BEST3), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu08218
Clone ID Related Accession (Same CDS sequence)	NM_001282615.1 , NM_001282615.2 , NM_001282616.2 , NM_001282616.1
Accession Version	NM_001282616.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA767581.1, BP225850.1, AC025263.22 and BC006440.1. Transcript Variant: This variant (6) lacks a portion of the 5' UTR and 5' coding region, uses a downstream in-frame start codon, and lacks several 3' exons but includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (5) is shorter at the N-terminus, has a distinct C-terminus and is significantly shorter than isoform 1. Both variants 5 and 6 encode isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BP225850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269545.1
CDS	379..597
Translation	MFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAERTGMKPILPSSFEMQSF

Target ORF information:

RefSeq Version	NM_001282616.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282616.1

ORF Insert Sequence:

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGAAAGAAC TGGCATGAAA 
CCCATTCTGC CTTCAAGTTT TGAGATGCAG AGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( XM_011537961.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536263.1 Homo sapiens bestrophin 3 (BEST3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu05038
Clone ID Related Accession (Same CDS sequence)	XM_011537961.1 , NM_001282613.1
Accession Version	XM_011537961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGGTTTTAT GACAACAGAT 
GAAAGGAAAT TATTCAACCA CCTCAAGTCT CCTCATCTGA AATATTGGGT TCCATTCATC 
TGGTTTGGAA ATCTTGCAAC TAAAGCCCGG AATGAAGGTA GAATCAGAGA CAGTGTTGAT 
CTGCAATCAT TGATGACTGA AATGAATCGA TACCGCTCTT GGTGCAGCCT CTTATTCGGT 
TATGACTGGG TTGGGATTCC GCTGGTTTAC ACCCAGGTTG TCACTCTTGC TGTCTATACC 
TTCTTCTTTG CGTGCCTGAT TGGACGCCAG TTTTTGGATC CCACCAAAGG CTACGCAGGG 
CATGACTTGG ATCTTTACAT TCCCATCTTC ACCCTCCTAC AATTCTTCTT CTATGCAGGA 
TGGCTTAAGG TAGCAGAGCA GCTTATCAAC CCTTTTGGAG AAGATGATGA TGATTTTGAA 
ACTAACTGGT GCATTGACAG AAATTTGCAG GTCTCTCTTT TAGCTGTGGA CGAAATGCAC 
ATGAGCTTAC CCAAGATGAA GAAGGACATT TACTGGGACG ATTCTGCTGC TCGCCCACCA 
TACACATTGG CAGCTGCTGA CTACTGCATA CCCTCATTTC TGGGGTCAAC AGTCCAGATG 
GGGCTGTCTG GGTCCGACTT TCCTGACGAG GAGTGGCTGT GGGATTATGA GAAGCATGGC 
CATCGGCATT CCATGATAAG AAGAGTCAAG CGGTTCCTGA GTGCCCACGA ACACCCCTCC 
AGCCCCAGAA GAAGAAGCTA CAGGAGGCAG ACAAGTGACA GCTCCATGTT CTTACCCCGA 
GATGACCTCA GCCCAGCCAG GGACCTACTG GATGTGCCCT CAAGAAACCC CCCCAGGGCC 
TCACCCACCT GGAAGAAATC CTGCTTCCCA GAAGGAAGCC CCACGCTGCA CTTCAGCATG 
GGAGAGCTGT CCACCATCAG GGAGACCAGC CAGACAAGCA CTTTACAGAG CCTGACCCCA 
CAGTCCAGTG TGAGAACTTC CCCCATCAAA ATGCCACTGG TACCTGAGGT ATTGATCACA 
GCAGCCGAAG CACCAGTGCC CACATCAGGG GGCTACCACC ATGATTCCGC TACCTCCATC 
TTGAGCTCTG AGTTTACAGG GGTTCAGCCA AGCAAGACTG AGCAGCAGCA GGGCCCCATG 
GGATCCATCC TGTCTCCCTC AGAGAAGGAG ACACCTCCTG GAGGCCCCAG TCCCCAGACA 
GTTTCAGCCA GCGCTGAGGA AAATATATTC AACTGTGAAG AAGACCCTGG TGATACCTTT 
CTAAAAAGGT GGAGTCTTCC GGGATTCCTG GGGTCCAGCC ACACTTCCCT GGGAAACCTA 
AGTCCAGACC CCATGAGCTC TCAGCCAGCT CTTTTAATTG ACACAGAAAC ATCCTCAGAG 
ATCAGTGGGA TCAACATTGT GGCTGGCTCT CGAGTCTCTT CTGATATGCT GTATTTAATG 
GAAAACCTGG ACACCAAGGA AACAGATATC ATAGAGCTGA ACAAGGAAAC TGAGGAATCA 
CCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536263.1
CDS	154..1842
Translation	MFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	XM_011537961.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537961.1

ORF Insert Sequence:

ATGTTCCTCA TCTCTAGCAG TGTTCACGGA AGCGACGAGC ACGGGCGCCT GCTTAGAAGG 
ACGCTGATGC GCTACGTCAA TCTCACCTCC CTGCTCATCT TTCGCTCGGT GAGCACTGCT 
GTGTACAAAA GATTTCCCAC AATGGACCAC GTGGTTGAAG CAGGTTTTAT GACAACAGAT 
GAAAGGAAAT TATTCAACCA CCTCAAGTCT CCTCATCTGA AATATTGGGT TCCATTCATC 
TGGTTTGGAA ATCTTGCAAC TAAAGCCCGG AATGAAGGTA GAATCAGAGA CAGTGTTGAT 
CTGCAATCAT TGATGACTGA AATGAATCGA TACCGCTCTT GGTGCAGCCT CTTATTCGGT 
TATGACTGGG TTGGGATTCC GCTGGTTTAC ACCCAGGTTG TCACTCTTGC TGTCTATACC 
TTCTTCTTTG CGTGCCTGAT TGGACGCCAG TTTTTGGATC CCACCAAAGG CTACGCAGGG 
CATGACTTGG ATCTTTACAT TCCCATCTTC ACCCTCCTAC AATTCTTCTT CTATGCAGGA 
TGGCTTAAGG TAGCAGAGCA GCTTATCAAC CCTTTTGGAG AAGATGATGA TGATTTTGAA 
ACTAACTGGT GCATTGACAG AAATTTGCAG GTCTCTCTTT TAGCTGTGGA CGAAATGCAC 
ATGAGCTTAC CCAAGATGAA GAAGGACATT TACTGGGACG ATTCTGCTGC TCGCCCACCA 
TACACATTGG CAGCTGCTGA CTACTGCATA CCCTCATTTC TGGGGTCAAC AGTCCAGATG 
GGGCTGTCTG GGTCCGACTT TCCTGACGAG GAGTGGCTGT GGGATTATGA GAAGCATGGC 
CATCGGCATT CCATGATAAG AAGAGTCAAG CGGTTCCTGA GTGCCCACGA ACACCCCTCC 
AGCCCCAGAA GAAGAAGCTA CAGGAGGCAG ACAAGTGACA GCTCCATGTT CTTACCCCGA 
GATGACCTCA GCCCAGCCAG GGACCTACTG GATGTGCCCT CAAGAAACCC CCCCAGGGCC 
TCACCCACCT GGAAGAAATC CTGCTTCCCA GAAGGAAGCC CCACGCTGCA CTTCAGCATG 
GGAGAGCTGT CCACCATCAG GGAGACCAGC CAGACAAGCA CTTTACAGAG CCTGACCCCA 
CAGTCCAGTG TGAGAACTTC CCCCATCAAA ATGCCACTGG TACCTGAGGT ATTGATCACA 
GCAGCCGAAG CACCAGTGCC CACATCAGGG GGCTACCACC ATGATTCCGC TACCTCCATC 
TTGAGCTCTG AGTTTACAGG GGTTCAGCCA AGCAAGACTG AGCAGCAGCA GGGCCCCATG 
GGATCCATCC TGTCTCCCTC AGAGAAGGAG ACACCTCCTG GAGGCCCCAG TCCCCAGACA 
GTTTCAGCCA GCGCTGAGGA AAATATATTC AACTGTGAAG AAGACCCTGG TGATACCTTT 
CTAAAAAGGT GGAGTCTTCC GGGATTCCTG GGGTCCAGCC ACACTTCCCT GGGAAACCTA 
AGTCCAGACC CCATGAGCTC TCAGCCAGCT CTTTTAATTG ACACAGAAAC ATCCTCAGAG 
ATCAGTGGGA TCAACATTGT GGCTGGCTCT CGAGTCTCTT CTGATATGCT GTATTTAATG 
GAAAACCTGG ACACCAAGGA AACAGATATC ATAGAGCTGA ACAAGGAAAC TGAGGAATCA 
CCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( XM_011537963.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536265.1 Homo sapiens bestrophin 3 (BEST3), transcript variant X7, mRNA.

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CloneID	OHu46341
Clone ID Related Accession (Same CDS sequence)	XM_011537963.1 , XM_011537962.2
Accession Version	XM_011537963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG 
GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA 
GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC 
CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT TGTCACTCTT 
GCTGTCTATA CCTTCTTCTT TGCGTGCCTG ATTGGACGCC AGTTTTTGGA TCCCACCAAA 
GGCTACGCAG GGCATGACTT GGATCTTTAC ATTCCCATCT TCACCCTCCT ACAATTCTTC 
TTCTATGCAG GATGGCTTAA GGTAGCAGAG CAGCTTATCA ACCCTTTTGG AGAAGATGAT 
GATGATTTTG AAACTAACTG GTGCATTGAC AGAAATTTGC AGGTCTCTCT TTTAGCTGTG 
GACGAAATGC ACATGAGCTT ACCCAAGATG AAGAAGGACA TTTACTGGGA CGATTCTGCT 
GCTCGCCCAC CATACACATT GGCAGCTGCT GACTACTGCA TACCCTCATT TCTGGGGTCA 
ACAGTCCAGA TGGGGCTGTC TGGGTCCGAC TTTCCTGACG AGGAGTGGCT GTGGGATTAT 
GAGAAGCATG GCCATCGGCA TTCCATGATA AGAAGAGTCA AGCGGTTCCT GAGTGCCCAC 
GAACACCCCT CCAGCCCCAG AAGAAGAAGC TACAGGAGGC AGACAAGTGA CAGCTCCATG 
TTCTTACCCC GAGATGACCT CAGCCCAGCC AGGGACCTAC TGGATGTGCC CTCAAGAAAC 
CCCCCCAGGG CCTCACCCAC CTGGAAGAAA TCCTGCTTCC CAGAAGGAAG CCCCACGCTG 
CACTTCAGCA TGGGAGAGCT GTCCACCATC AGGGAGACCA GCCAGACAAG CACTTTACAG 
AGCCTGACCC CACAGTCCAG TGTGAGAACT TCCCCCATCA AAATGCCACT GGTACCTGAG 
GTATTGATCA CAGCAGCCGA AGCACCAGTG CCCACATCAG GGGGCTACCA CCATGATTCC 
GCTACCTCCA TCTTGAGCTC TGAGTTTACA GGGGTTCAGC CAAGCAAGAC TGAGCAGCAG 
CAGGGCCCCA TGGGATCCAT CCTGTCTCCC TCAGAGAAGG AGACACCTCC TGGAGGCCCC 
AGTCCCCAGA CAGTTTCAGC CAGCGCTGAG GAAAATATAT TCAACTGTGA AGAAGACCCT 
GGTGATACCT TTCTAAAAAG GTGGAGTCTT CCGGGATTCC TGGGGTCCAG CCACACTTCC 
CTGGGAAACC TAAGTCCAGA CCCCATGAGC TCTCAGCCAG CTCTTTTAAT TGACACAGAA 
ACATCCTCAG AGATCAGTGG GATCAACATT GTGGCTGGCT CTCGAGTCTC TTCTGATATG 
CTGTATTTAA TGGAAAACCT GGACACCAAG GAAACAGATA TCATAGAGCT GAACAAGGAA 
ACTGAGGAAT CACCCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536265.1
CDS	93..1613
Translation	MTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	XM_011537963.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537963.1

ORF Insert Sequence:

ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG 
GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA 
GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC 
CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT TGTCACTCTT 
GCTGTCTATA CCTTCTTCTT TGCGTGCCTG ATTGGACGCC AGTTTTTGGA TCCCACCAAA 
GGCTACGCAG GGCATGACTT GGATCTTTAC ATTCCCATCT TCACCCTCCT ACAATTCTTC 
TTCTATGCAG GATGGCTTAA GGTAGCAGAG CAGCTTATCA ACCCTTTTGG AGAAGATGAT 
GATGATTTTG AAACTAACTG GTGCATTGAC AGAAATTTGC AGGTCTCTCT TTTAGCTGTG 
GACGAAATGC ACATGAGCTT ACCCAAGATG AAGAAGGACA TTTACTGGGA CGATTCTGCT 
GCTCGCCCAC CATACACATT GGCAGCTGCT GACTACTGCA TACCCTCATT TCTGGGGTCA 
ACAGTCCAGA TGGGGCTGTC TGGGTCCGAC TTTCCTGACG AGGAGTGGCT GTGGGATTAT 
GAGAAGCATG GCCATCGGCA TTCCATGATA AGAAGAGTCA AGCGGTTCCT GAGTGCCCAC 
GAACACCCCT CCAGCCCCAG AAGAAGAAGC TACAGGAGGC AGACAAGTGA CAGCTCCATG 
TTCTTACCCC GAGATGACCT CAGCCCAGCC AGGGACCTAC TGGATGTGCC CTCAAGAAAC 
CCCCCCAGGG CCTCACCCAC CTGGAAGAAA TCCTGCTTCC CAGAAGGAAG CCCCACGCTG 
CACTTCAGCA TGGGAGAGCT GTCCACCATC AGGGAGACCA GCCAGACAAG CACTTTACAG 
AGCCTGACCC CACAGTCCAG TGTGAGAACT TCCCCCATCA AAATGCCACT GGTACCTGAG 
GTATTGATCA CAGCAGCCGA AGCACCAGTG CCCACATCAG GGGGCTACCA CCATGATTCC 
GCTACCTCCA TCTTGAGCTC TGAGTTTACA GGGGTTCAGC CAAGCAAGAC TGAGCAGCAG 
CAGGGCCCCA TGGGATCCAT CCTGTCTCCC TCAGAGAAGG AGACACCTCC TGGAGGCCCC 
AGTCCCCAGA CAGTTTCAGC CAGCGCTGAG GAAAATATAT TCAACTGTGA AGAAGACCCT 
GGTGATACCT TTCTAAAAAG GTGGAGTCTT CCGGGATTCC TGGGGTCCAG CCACACTTCC 
CTGGGAAACC TAAGTCCAGA CCCCATGAGC TCTCAGCCAG CTCTTTTAAT TGACACAGAA 
ACATCCTCAG AGATCAGTGG GATCAACATT GTGGCTGGCT CTCGAGTCTC TTCTGATATG 
CTGTATTTAA TGGAAAACCT GGACACCAAG GAAACAGATA TCATAGAGCT GAACAAGGAA 
ACTGAGGAAT CACCCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( XM_011537962.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536264.1 Homo sapiens bestrophin 3 (BEST3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu46341
Clone ID Related Accession (Same CDS sequence)	XM_011537963.1 , XM_011537962.2
Accession Version	XM_011537962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG 
GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA 
GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC 
CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT TGTCACTCTT 
GCTGTCTATA CCTTCTTCTT TGCGTGCCTG ATTGGACGCC AGTTTTTGGA TCCCACCAAA 
GGCTACGCAG GGCATGACTT GGATCTTTAC ATTCCCATCT TCACCCTCCT ACAATTCTTC 
TTCTATGCAG GATGGCTTAA GGTAGCAGAG CAGCTTATCA ACCCTTTTGG AGAAGATGAT 
GATGATTTTG AAACTAACTG GTGCATTGAC AGAAATTTGC AGGTCTCTCT TTTAGCTGTG 
GACGAAATGC ACATGAGCTT ACCCAAGATG AAGAAGGACA TTTACTGGGA CGATTCTGCT 
GCTCGCCCAC CATACACATT GGCAGCTGCT GACTACTGCA TACCCTCATT TCTGGGGTCA 
ACAGTCCAGA TGGGGCTGTC TGGGTCCGAC TTTCCTGACG AGGAGTGGCT GTGGGATTAT 
GAGAAGCATG GCCATCGGCA TTCCATGATA AGAAGAGTCA AGCGGTTCCT GAGTGCCCAC 
GAACACCCCT CCAGCCCCAG AAGAAGAAGC TACAGGAGGC AGACAAGTGA CAGCTCCATG 
TTCTTACCCC GAGATGACCT CAGCCCAGCC AGGGACCTAC TGGATGTGCC CTCAAGAAAC 
CCCCCCAGGG CCTCACCCAC CTGGAAGAAA TCCTGCTTCC CAGAAGGAAG CCCCACGCTG 
CACTTCAGCA TGGGAGAGCT GTCCACCATC AGGGAGACCA GCCAGACAAG CACTTTACAG 
AGCCTGACCC CACAGTCCAG TGTGAGAACT TCCCCCATCA AAATGCCACT GGTACCTGAG 
GTATTGATCA CAGCAGCCGA AGCACCAGTG CCCACATCAG GGGGCTACCA CCATGATTCC 
GCTACCTCCA TCTTGAGCTC TGAGTTTACA GGGGTTCAGC CAAGCAAGAC TGAGCAGCAG 
CAGGGCCCCA TGGGATCCAT CCTGTCTCCC TCAGAGAAGG AGACACCTCC TGGAGGCCCC 
AGTCCCCAGA CAGTTTCAGC CAGCGCTGAG GAAAATATAT TCAACTGTGA AGAAGACCCT 
GGTGATACCT TTCTAAAAAG GTGGAGTCTT CCGGGATTCC TGGGGTCCAG CCACACTTCC 
CTGGGAAACC TAAGTCCAGA CCCCATGAGC TCTCAGCCAG CTCTTTTAAT TGACACAGAA 
ACATCCTCAG AGATCAGTGG GATCAACATT GTGGCTGGCT CTCGAGTCTC TTCTGATATG 
CTGTATTTAA TGGAAAACCT GGACACCAAG GAAACAGATA TCATAGAGCT GAACAAGGAA 
ACTGAGGAAT CACCCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536264.1
CDS	85..1605
Translation	MTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	XM_011537962.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537962.2

ORF Insert Sequence:

ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG 
GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA 
GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC 
CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT TGTCACTCTT 
GCTGTCTATA CCTTCTTCTT TGCGTGCCTG ATTGGACGCC AGTTTTTGGA TCCCACCAAA 
GGCTACGCAG GGCATGACTT GGATCTTTAC ATTCCCATCT TCACCCTCCT ACAATTCTTC 
TTCTATGCAG GATGGCTTAA GGTAGCAGAG CAGCTTATCA ACCCTTTTGG AGAAGATGAT 
GATGATTTTG AAACTAACTG GTGCATTGAC AGAAATTTGC AGGTCTCTCT TTTAGCTGTG 
GACGAAATGC ACATGAGCTT ACCCAAGATG AAGAAGGACA TTTACTGGGA CGATTCTGCT 
GCTCGCCCAC CATACACATT GGCAGCTGCT GACTACTGCA TACCCTCATT TCTGGGGTCA 
ACAGTCCAGA TGGGGCTGTC TGGGTCCGAC TTTCCTGACG AGGAGTGGCT GTGGGATTAT 
GAGAAGCATG GCCATCGGCA TTCCATGATA AGAAGAGTCA AGCGGTTCCT GAGTGCCCAC 
GAACACCCCT CCAGCCCCAG AAGAAGAAGC TACAGGAGGC AGACAAGTGA CAGCTCCATG 
TTCTTACCCC GAGATGACCT CAGCCCAGCC AGGGACCTAC TGGATGTGCC CTCAAGAAAC 
CCCCCCAGGG CCTCACCCAC CTGGAAGAAA TCCTGCTTCC CAGAAGGAAG CCCCACGCTG 
CACTTCAGCA TGGGAGAGCT GTCCACCATC AGGGAGACCA GCCAGACAAG CACTTTACAG 
AGCCTGACCC CACAGTCCAG TGTGAGAACT TCCCCCATCA AAATGCCACT GGTACCTGAG 
GTATTGATCA CAGCAGCCGA AGCACCAGTG CCCACATCAG GGGGCTACCA CCATGATTCC 
GCTACCTCCA TCTTGAGCTC TGAGTTTACA GGGGTTCAGC CAAGCAAGAC TGAGCAGCAG 
CAGGGCCCCA TGGGATCCAT CCTGTCTCCC TCAGAGAAGG AGACACCTCC TGGAGGCCCC 
AGTCCCCAGA CAGTTTCAGC CAGCGCTGAG GAAAATATAT TCAACTGTGA AGAAGACCCT 
GGTGATACCT TTCTAAAAAG GTGGAGTCTT CCGGGATTCC TGGGGTCCAG CCACACTTCC 
CTGGGAAACC TAAGTCCAGA CCCCATGAGC TCTCAGCCAG CTCTTTTAAT TGACACAGAA 
ACATCCTCAG AGATCAGTGG GATCAACATT GTGGCTGGCT CTCGAGTCTC TTCTGATATG 
CTGTATTTAA TGGAAAACCT GGACACCAAG GAAACAGATA TCATAGAGCT GAACAAGGAA 
ACTGAGGAAT CACCCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( XM_011537965.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536267.1 Homo sapiens bestrophin 3 (BEST3), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu76091
Clone ID Related Accession (Same CDS sequence)	XM_011537965.2
Accession Version	XM_011537965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATGA GCTTACCCAA GATGAAGAAG GACATTTACT GGGACGATTC TGCTGCTCGC 
CCACCATACA CATTGGCAGC TGCTGACTAC TGCATACCCT CATTTCTGGG GTCAACAGTC 
CAGATGGGGC TGTCTGGGTC CGACTTTCCT GACGAGGAGT GGCTGTGGGA TTATGAGAAG 
CATGGCCATC GGCATTCCAT GATAAGAAGA GTCAAGCGGT TCCTGAGTGC CCACGAACAC 
CCCTCCAGCC CCAGAAGAAG AAGCTACAGG AGGCAGACAA GTGACAGCTC CATGTTCTTA 
CCCCGAGATG ACCTCAGCCC AGCCAGGGAC CTACTGGATG TGCCCTCAAG AAACCCCCCC 
AGGGCCTCAC CCACCTGGAA GAAATCCTGC TTCCCAGAAG GAAGCCCCAC GCTGCACTTC 
AGCATGGGAG AGCTGTCCAC CATCAGGGAG ACCAGCCAGA CAAGCACTTT ACAGAGCCTG 
ACCCCACAGT CCAGTGTGAG AACTTCCCCC ATCAAAATGC CACTGGTACC TGAGGTATTG 
ATCACAGCAG CCGAAGCACC AGTGCCCACA TCAGGGGGCT ACCACCATGA TTCCGCTACC 
TCCATCTTGA GCTCTGAGTT TACAGGGGTT CAGCCAAGCA AGACTGAGCA GCAGCAGGGC 
CCCATGGGAT CCATCCTGTC TCCCTCAGAG AAGGAGACAC CTCCTGGAGG CCCCAGTCCC 
CAGACAGTTT CAGCCAGCGC TGAGGAAAAT ATATTCAACT GTGAAGAAGA CCCTGGTGAT 
ACCTTTCTAA AAAGGTGGAG TCTTCCGGGA TTCCTGGGGT CCAGCCACAC TTCCCTGGGA 
AACCTAAGTC CAGACCCCAT GAGCTCTCAG CCAGCTCTTT TAATTGACAC AGAAACATCC 
TCAGAGATCA GTGGGATCAA CATTGTGGCT GGCTCTCGAG TCTCTTCTGA TATGCTGTAT 
TTAATGGAAA ACCTGGACAC CAAGGAAACA GATATCATAG AGCTGAACAA GGAAACTGAG 
GAATCACCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536267.1
CDS	318..1352
Translation	MHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	XM_011537965.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537965.2

ORF Insert Sequence:

ATGCACATGA GCTTACCCAA GATGAAGAAG GACATTTACT GGGACGATTC TGCTGCTCGC 
CCACCATACA CATTGGCAGC TGCTGACTAC TGCATACCCT CATTTCTGGG GTCAACAGTC 
CAGATGGGGC TGTCTGGGTC CGACTTTCCT GACGAGGAGT GGCTGTGGGA TTATGAGAAG 
CATGGCCATC GGCATTCCAT GATAAGAAGA GTCAAGCGGT TCCTGAGTGC CCACGAACAC 
CCCTCCAGCC CCAGAAGAAG AAGCTACAGG AGGCAGACAA GTGACAGCTC CATGTTCTTA 
CCCCGAGATG ACCTCAGCCC AGCCAGGGAC CTACTGGATG TGCCCTCAAG AAACCCCCCC 
AGGGCCTCAC CCACCTGGAA GAAATCCTGC TTCCCAGAAG GAAGCCCCAC GCTGCACTTC 
AGCATGGGAG AGCTGTCCAC CATCAGGGAG ACCAGCCAGA CAAGCACTTT ACAGAGCCTG 
ACCCCACAGT CCAGTGTGAG AACTTCCCCC ATCAAAATGC CACTGGTACC TGAGGTATTG 
ATCACAGCAG CCGAAGCACC AGTGCCCACA TCAGGGGGCT ACCACCATGA TTCCGCTACC 
TCCATCTTGA GCTCTGAGTT TACAGGGGTT CAGCCAAGCA AGACTGAGCA GCAGCAGGGC 
CCCATGGGAT CCATCCTGTC TCCCTCAGAG AAGGAGACAC CTCCTGGAGG CCCCAGTCCC 
CAGACAGTTT CAGCCAGCGC TGAGGAAAAT ATATTCAACT GTGAAGAAGA CCCTGGTGAT 
ACCTTTCTAA AAAGGTGGAG TCTTCCGGGA TTCCTGGGGT CCAGCCACAC TTCCCTGGGA 
AACCTAAGTC CAGACCCCAT GAGCTCTCAG CCAGCTCTTT TAATTGACAC AGAAACATCC 
TCAGAGATCA GTGGGATCAA CATTGTGGCT GGCTCTCGAG TCTCTTCTGA TATGCTGTAT 
TTAATGGAAA ACCTGGACAC CAAGGAAACA GATATCATAG AGCTGAACAA GGAAACTGAG 
GAATCACCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_001282614.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269543.1 Homo sapiens bestrophin 3 (BEST3), transcript variant 4, mRNA.

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CloneID	OHu00668
Clone ID Related Accession (Same CDS sequence)	NM_001282614.1
Accession Version	NM_001282614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA767581.1, AF440758.1, AY515706.1 and BX374313.2. On Sep 14, 2013 this sequence version replaced XM_005268673.1. Transcript Variant: This variant (4) contains an alternate 3' terminal exon and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (4) has a distinct and shorter C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF440758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END##

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GAAACAGATG CCTAAGAATG AGTGGAAGAT GGAAGATATA 
AAAATCCCAC TCCCTCAGCC TCAATTCCAG TGTGCTAAGA GTGATCCTGG TGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269543.1
CDS	228..1424
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGKQMPKNEWKMEDIKIPLPQPQFQCAKSDPGG

Target ORF information:

RefSeq Version	NM_001282614.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282614.1

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GAAACAGATG CCTAAGAATG AGTGGAAGAT GGAAGATATA 
AAAATCCCAC TCCCTCAGCC TCAATTCCAG TGTGCTAAGA GTGATCCTGG TGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( NM_152439.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689652.2 Homo sapiens bestrophin 3 (BEST3), transcript variant 2, mRNA.

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CloneID	OHu06779
Clone ID Related Accession (Same CDS sequence)	NM_152439.3
Accession Version	NM_152439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC028087.1, BF739974.1 and AK096459.1. On Sep 14, 2013 this sequence version replaced NM_152439.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream in-frame start codon, and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC028087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG 
GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA 
GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC 
CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT AGCAGAGCAG 
CTTATCAACC CTTTTGGAGA AGATGATGAT GATTTTGAAA CTAACTGGTG CATTGACAGA 
AATTTGCAGG TCTCTCTTTT AGCTGTGGAC GAAATGCACA TGAGCTTACC CAAGATGAAG 
AAGGACATTT ACTGGGACGA TTCTGCTGCT CGCCCACCAT ACACATTGGC AGCTGCTGAC 
TACTGCATAC CCTCATTTCT GGGGTCAACA GTCCAGATGG GGCTGTCTGG GTCCGACTTT 
CCTGACGAGG AGTGGCTGTG GGATTATGAG AAGCATGGCC ATCGGCATTC CATGATAAGA 
AGAGTCAAGC GGTTCCTGAG TGCCCACGAA CACCCCTCCA GCCCCAGAAG AAGAAGCTAC 
AGGAGGCAGA CAAGTGACAG CTCCATGTTC TTACCCCGAG ATGACCTCAG CCCAGCCAGG 
GACCTACTGG ATGTGCCCTC AAGAAACCCC CCCAGGGCCT CACCCACCTG GAAGAAATCC 
TGCTTCCCAG AAGGAAGCCC CACGCTGCAC TTCAGCATGG GAGAGCTGTC CACCATCAGG 
GAGACCAGCC AGACAAGCAC TTTACAGAGC CTGACCCCAC AGTCCAGTGT GAGAACTTCC 
CCCATCAAAA TGCCACTGGT ACCTGAGGTA TTGATCACAG CAGCCGAAGC ACCAGTGCCC 
ACATCAGGGG GCTACCACCA TGATTCCGCT ACCTCCATCT TGAGCTCTGA GTTTACAGGG 
GTTCAGCCAA GCAAGACTGA GCAGCAGCAG GGCCCCATGG GATCCATCCT GTCTCCCTCA 
GAGAAGGAGA CACCTCCTGG AGGCCCCAGT CCCCAGACAG TTTCAGCCAG CGCTGAGGAA 
AATATATTCA ACTGTGAAGA AGACCCTGGT GATACCTTTC TAAAAAGGTG GAGTCTTCCG 
GGATTCCTGG GGTCCAGCCA CACTTCCCTG GGAAACCTAA GTCCAGACCC CATGAGCTCT 
CAGCCAGCTC TTTTAATTGA CACAGAAACA TCCTCAGAGA TCAGTGGGAT CAACATTGTG 
GCTGGCTCTC GAGTCTCTTC TGATATGCTG TATTTAATGG AAAACCTGGA CACCAAGGAA 
ACAGATATCA TAGAGCTGAA CAAGGAAACT GAGGAATCAC CCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689652.2
CDS	214..1581
Translation	MTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMFLPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK

Target ORF information:

RefSeq Version	NM_152439.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152439.3

ORF Insert Sequence:

ATGACAACAG ATGAAAGGAA ATTATTCAAC CACCTCAAGT CTCCTCATCT GAAATATTGG 
GTTCCATTCA TCTGGTTTGG AAATCTTGCA ACTAAAGCCC GGAATGAAGG TAGAATCAGA 
GACAGTGTTG ATCTGCAATC ATTGATGACT GAAATGAATC GATACCGCTC TTGGTGCAGC 
CTCTTATTCG GTTATGACTG GGTTGGGATT CCGCTGGTTT ACACCCAGGT AGCAGAGCAG 
CTTATCAACC CTTTTGGAGA AGATGATGAT GATTTTGAAA CTAACTGGTG CATTGACAGA 
AATTTGCAGG TCTCTCTTTT AGCTGTGGAC GAAATGCACA TGAGCTTACC CAAGATGAAG 
AAGGACATTT ACTGGGACGA TTCTGCTGCT CGCCCACCAT ACACATTGGC AGCTGCTGAC 
TACTGCATAC CCTCATTTCT GGGGTCAACA GTCCAGATGG GGCTGTCTGG GTCCGACTTT 
CCTGACGAGG AGTGGCTGTG GGATTATGAG AAGCATGGCC ATCGGCATTC CATGATAAGA 
AGAGTCAAGC GGTTCCTGAG TGCCCACGAA CACCCCTCCA GCCCCAGAAG AAGAAGCTAC 
AGGAGGCAGA CAAGTGACAG CTCCATGTTC TTACCCCGAG ATGACCTCAG CCCAGCCAGG 
GACCTACTGG ATGTGCCCTC AAGAAACCCC CCCAGGGCCT CACCCACCTG GAAGAAATCC 
TGCTTCCCAG AAGGAAGCCC CACGCTGCAC TTCAGCATGG GAGAGCTGTC CACCATCAGG 
GAGACCAGCC AGACAAGCAC TTTACAGAGC CTGACCCCAC AGTCCAGTGT GAGAACTTCC 
CCCATCAAAA TGCCACTGGT ACCTGAGGTA TTGATCACAG CAGCCGAAGC ACCAGTGCCC 
ACATCAGGGG GCTACCACCA TGATTCCGCT ACCTCCATCT TGAGCTCTGA GTTTACAGGG 
GTTCAGCCAA GCAAGACTGA GCAGCAGCAG GGCCCCATGG GATCCATCCT GTCTCCCTCA 
GAGAAGGAGA CACCTCCTGG AGGCCCCAGT CCCCAGACAG TTTCAGCCAG CGCTGAGGAA 
AATATATTCA ACTGTGAAGA AGACCCTGGT GATACCTTTC TAAAAAGGTG GAGTCTTCCG 
GGATTCCTGG GGTCCAGCCA CACTTCCCTG GGAAACCTAA GTCCAGACCC CATGAGCTCT 
CAGCCAGCTC TTTTAATTGA CACAGAAACA TCCTCAGAGA TCAGTGGGAT CAACATTGTG 
GCTGGCTCTC GAGTCTCTTC TGATATGCTG TATTTAATGG AAAACCTGGA CACCAAGGAA 
ACAGATATCA TAGAGCTGAA CAAGGAAACT GAGGAATCAC CCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( XM_006719256.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719319.1 Homo sapiens bestrophin 3 (BEST3), transcript variant X4, mRNA.

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CloneID	OHu46342
Clone ID Related Accession (Same CDS sequence)	XM_006719256.3
Accession Version	XM_006719256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GAGATCTCGC TTCAACCCTG TTCCCTTTGC CGAATGTCTC 
CTAATCCCCA TCAGTGATGG TTTGTGGCAC TTCTCTTCCA AAGGGGCCTC TGAGGCTGAA 
AACCTTTCCA GACCCAAAGA AAAGGAAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719319.1
CDS	16..1248
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQMGRSRFNPVPFAECLLIPISDGLWHFSSKGASEAENLSRPKEKED

Target ORF information:

RefSeq Version	XM_006719256.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719256.3

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GGTTTTATGA CAACAGATGA AAGGAAATTA TTCAACCACC TCAAGTCTCC TCATCTGAAA 
TATTGGGTTC CATTCATCTG GTTTGGAAAT CTTGCAACTA AAGCCCGGAA TGAAGGTAGA 
ATCAGAGACA GTGTTGATCT GCAATCATTG ATGACTGAAA TGAATCGATA CCGCTCTTGG 
TGCAGCCTCT TATTCGGTTA TGACTGGGTT GGGATTCCGC TGGTTTACAC CCAGGTTGTC 
ACTCTTGCTG TCTATACCTT CTTCTTTGCG TGCCTGATTG GACGCCAGTT TTTGGATCCC 
ACCAAAGGCT ACGCAGGGCA TGACTTGGAT CTTTACATTC CCATCTTCAC CCTCCTACAA 
TTCTTCTTCT ATGCAGGATG GCTTAAGGTA GCAGAGCAGC TTATCAACCC TTTTGGAGAA 
GATGATGATG ATTTTGAAAC TAACTGGTGC ATTGACAGAA ATTTGCAGGT CTCTCTTTTA 
GCTGTGGACG AAATGCACAT GAGCTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAT 
TCTGCTGCTC GCCCACCATA CACATTGGCA GCTGCTGACT ACTGCATACC CTCATTTCTG 
GGGTCAACAG TCCAGATGGG GAGATCTCGC TTCAACCCTG TTCCCTTTGC CGAATGTCTC 
CTAATCCCCA TCAGTGATGG TTTGTGGCAC TTCTCTTCCA AAGGGGCCTC TGAGGCTGAA 
AACCTTTCCA GACCCAAAGA AAAGGAAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST3 ( XM_017018865.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874354.1 Homo sapiens bestrophin 3 (BEST3), transcript variant X7, mRNA.

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CloneID	OHu83546
Clone ID Related Accession (Same CDS sequence)	XM_017018865.1
Accession Version	XM_017018865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GAAAGAACTG GCATGAAACC CATTCTGCCT TCAAGTTTTG AGATGCAGAG CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874354.1
CDS	16..552
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGVQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDEHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAERTGMKPILPSSFEMQSF

Target ORF information:

RefSeq Version	XM_017018865.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 3 (BEST3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018865.1

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCAACTT TTTTTGGATT TCATAGGTTA 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTACTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTATA CAGCAATAAG TTTGGTATAC AGATTGTTAC TTACAGGAGT CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTATGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATTTG 
CCCTGGCCAG ACAGGCTAAT GTTCCTCATC TCTAGCAGTG TTCACGGAAG CGACGAGCAC 
GGGCGCCTGC TTAGAAGGAC GCTGATGCGC TACGTCAATC TCACCTCCCT GCTCATCTTT 
CGCTCGGTGA GCACTGCTGT GTACAAAAGA TTTCCCACAA TGGACCACGT GGTTGAAGCA 
GAAAGAACTG GCATGAAACC CATTCTGCCT TCAAGTTTTG AGATGCAGAG CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	BEST3
Entrez Gene ID	144453
Full Name	bestrophin 3
Synonyms	VMD2L3
General protein information	Preferred Names bestrophin 3 Names bestrophin-3 vitelliform macular dystrophy 2-like 3 vitelliform macular dystrophy 2-like protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q15
Summary	BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM, Mar 2008].
Disorder MIM:	607337

	BEST3	XP_001117392.1
Pan troglodytes (chimpanzee)	BEST3	XP_522466.2
Mus musculus (house mouse)	Best3	NP_001007584.1
Rattus norvegicus (Norway rat)	Best3	NP_001178712.1
Canis lupus familiaris (dog)	BEST3	XP_538279.3
Gallus gallus (chicken)	BEST3	NP_001186598.1
Xenopus tropicalis (tropical clawed frog)	best3	NP_001155224.1
Bos taurus (cattle)	BEST3	XP_002687445.1
Homo sapiens (human)	BEST3	NP_116124.2
Drosophila melanogaster (fruit fly)	Best3	NP_730039.1

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BEST3 cDNA ORF clone, Homo sapiens(human)

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