ALG10B cDNA ORF clone, Homo sapiens(human)

The following ALG10B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALG10B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu30757 NM_001013620.3
Latest version!
Homo sapiens ALG10B, alpha-1,2-glucosyltransferase (ALG10B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu76083 NM_001308340.1
Latest version!
Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu46327 XM_005268665.4
Latest version!
Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu107245 NM_001013620.4
Latest version!
Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu108222 NM_001308340.2
Latest version!
Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu30757
    Clone ID Related Accession (Same CDS sequence) NM_001013620.3
    Accession Version NM_001013620.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB254106.1, AY845858.1, AC117372.2 and BQ024224.1. On Apr 20, 2007 this sequence version replaced NM_001013620.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DB254106.1, SRR1163655.398127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG GATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG CTGCCTCAAG TATCCAGAGA GTCTTGTCAA CATTAACACT AGCAGTATTT
    CCAACACTTT ATTTTTTTAA CTTCCTTTAT TATACAGAAG CAGGATCTAT GTTTTTTACT
    CTTTTTGCAT ATTTGATGTG TCTTTATGGA AATCATAAAA CTTCAGCCTT CCTTGGATTT
    TGTGGCTTCA TGTTTCGGCA AACAAATATC ATCTGGGCTG TCTTCTGTGC AGGGAATGTC
    ATTGCACAAA AGTTAACTGA GGCTTGGAAA ACTGAGCTAC AAAAGAAGGA AGACAGACTT
    CCACCTATTA AAGGACCATT TGCAGAATTC AGAAAAATTC TTCAGTTTCT TTTGGCTTAT
    TCCATGTCCT TTAAAAACTT GAGTATGCTT TTCTGTTTGA CTTGGCCCTA CATCCTTCTG
    GGATTTCTGT TTTGTGCTTT TGTAGTAGTT AATGGTGGAA TTGTTATTGG CGATCGGAGT
    AGTCATGAAG CCTGTCTTCA TTTTCCTCAA CTATTCTACT TTTTTTCATT TACTCTCTTT
    TTTTCTTTTC CTCATCTCCT GTCTCCTAGC AAAATTAAGA CTTTTCTTTC CTTAGTTTGG
    AAACATGGAA TTCTGTTTTT GGTGGTTACC TTAGTCTCTG TGTTTTTAGT TTGGAAATTC
    ACTTATGCTC ATAAATACTT GCTAGCAGAC AATAGACATT ATACTTTCTA TGTGTGGAAA
    AGAGTTTTTC AAAGATATGC AATTCTGAAA TATTTGTTAG TTCCAGCCTA TATATTTGCT
    GGTTGGAGTA TAGCTGACTC ATTGAAATCA AAGCCAATTT TTTGGAATTT AATGTTTTTC
    ATATGCTTGT TCATTGTTAT AGTTCCTCAG AAACTGCTGG AATTTCGTTA CTTCATTTTA
    CCTTATGTCA TTTATAGGCT TAACATAACT CTGCCTCCCA CATCCAGACT TGTTTGTGAA
    CTGAGTTGCT ATGCAATTGT TAATTTCATA ACTTTTTACA TCTTTCTGAA CAAGACTTTT
    CAGTGGCCAA ATAGTCAGGA CATTCAAAGG TTTATGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001013642.1
    CDS140..1561
    Translation

    Target ORF information:

    RefSeq Version NM_001013620.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ALG10B, alpha-1,2-glucosyltransferase (ALG10B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001013620.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG GATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG CTGCCTCAAG TATCCAGAGA GTCTTGTCAA CATTAACACT AGCAGTATTT
    CCAACACTTT ATTTTTTTAA CTTCCTTTAT TATACAGAAG CAGGATCTAT GTTTTTTACT
    CTTTTTGCAT ATTTGATGTG TCTTTATGGA AATCATAAAA CTTCAGCCTT CCTTGGATTT
    TGTGGCTTCA TGTTTCGGCA AACAAATATC ATCTGGGCTG TCTTCTGTGC AGGGAATGTC
    ATTGCACAAA AGTTAACTGA GGCTTGGAAA ACTGAGCTAC AAAAGAAGGA AGACAGACTT
    CCACCTATTA AAGGACCATT TGCAGAATTC AGAAAAATTC TTCAGTTTCT TTTGGCTTAT
    TCCATGTCCT TTAAAAACTT GAGTATGCTT TTCTGTTTGA CTTGGCCCTA CATCCTTCTG
    GGATTTCTGT TTTGTGCTTT TGTAGTAGTT AATGGTGGAA TTGTTATTGG CGATCGGAGT
    AGTCATGAAG CCTGTCTTCA TTTTCCTCAA CTATTCTACT TTTTTTCATT TACTCTCTTT
    TTTTCTTTTC CTCATCTCCT GTCTCCTAGC AAAATTAAGA CTTTTCTTTC CTTAGTTTGG
    AAACATGGAA TTCTGTTTTT GGTGGTTACC TTAGTCTCTG TGTTTTTAGT TTGGAAATTC
    ACTTATGCTC ATAAATACTT GCTAGCAGAC AATAGACATT ATACTTTCTA TGTGTGGAAA
    AGAGTTTTTC AAAGATATGC AATTCTGAAA TATTTGTTAG TTCCAGCCTA TATATTTGCT
    GGTTGGAGTA TAGCTGACTC ATTGAAATCA AAGCCAATTT TTTGGAATTT AATGTTTTTC
    ATATGCTTGT TCATTGTTAT AGTTCCTCAG AAACTGCTGG AATTTCGTTA CTTCATTTTA
    CCTTATGTCA TTTATAGGCT TAACATAACT CTGCCTCCCA CATCCAGACT TGTTTGTGAA
    CTGAGTTGCT ATGCAATTGT TAATTTCATA ACTTTTTACA TCTTTCTGAA CAAGACTTTT
    CAGTGGCCAA ATAGTCAGGA CATTCAAAGG TTTATGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76083
    Clone ID Related Accession (Same CDS sequence) NM_001308340.1
    Accession Version NM_001308340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-10
    Organism Homo sapiens(human)
    Product putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB254106.1, AL712776.1, AC117372.2 and BQ024224.1. On Apr 30, 2015 this sequence version replaced XM_011537948.1. Transcript Variant: This variant (2) uses an alternate splice site in its 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.78151.1, AL712776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG GATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG AATACTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001295269.1
    CDS140..520
    Translation

    Target ORF information:

    RefSeq Version NM_001308340.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG GATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG AATACTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46327
    Clone ID Related Accession (Same CDS sequence) XM_005268665.4
    Accession Version XM_005268665.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT 
    TGGATCTTTG CATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG CTGCCTCAAG TATCCAGAGA GTCTTGTCAA CATTAACACT AGCAGTATTT
    CCAACACTTT ATTTTTTTAA CTTCCTTTAT TATACAGAAG CAGGATCTAT GTTTTTTACT
    CTTTTTGCAT ATTTGATGTG TCTTTATGGA AATCATAAAA CTTCAGCCTT CCTTGGATTT
    TGTGGCTTCA TGTTTCGGCA AACAAATATC ATCTGGGCTG TCTTCTGTGC AGGGAATGTC
    ATTGCACAAA AGTTAACTGA GGCTTGGAAA ACTGAGCTAC AAAAGAAGGA AGACAGACTT
    CCACCTATTA AAGGACCATT TGCAGAATTC AGAAAAATTC TTCAGTTTCT TTTGGCTTAT
    TCCATGTCCT TTAAAAACTT GAGTATGCTT TTCTGTTTGA CTTGGCCCTA CATCCTTCTG
    GGATTTCTGT TTTGTGCTTT TGTAGTAGTT AATGGTGGAA TTGTTATTGG CGATCGGAGT
    AGTCATGAAG CCTGTCTTCA TTTTCCTCAA CTATTCTACT TTTTTTCATT TACTCTCTTT
    TTTTCTTTTC CTCATCTCCT GTCTCCTAGC AAAATTAAGA CTTTTCTTTC CTTAGTTTGG
    AAACATGGAA TTCTGTTTTT GGTGGTTACC TTAGTCTCTG TGTTTTTAGT TTGGAAATTC
    ACTTATGCTC ATAAATACTT GCTAGCAGAC AATAGACATT ATACTTTCTA TGTGTGGAAA
    AGAGTTTTTC AAAGATATGC AATTCTGAAA TATTTGTTAG TTCCAGCCTA TATATTTGCT
    GGTTGGAGTA TAGCTGACTC ATTGAAATCA AAGCCAATTT TTTGGAATTT AATGTTTTTC
    ATATGCTTGT TCATTGTTAT AGTTCCTCAG AAACTGCTGG AATTTCGTTA CTTCATTTTA
    CCTTATGTCA TTTATAGGCT TAACATAACT CTGCCTCCCA CATCCAGACT TGTTTGTGAA
    CTGAGTTGCT ATGCAATTGT TAATTTCATA ACTTTTTACA TCTTTCTGAA CAAGACTTTT
    CAGTGGCCAA ATAGTCAGGA CATTCAAAGG TTTATGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005268722.1
    CDS101..1342
    Translation

    Target ORF information:

    RefSeq Version XM_005268665.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005268665.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT 
    TGGATCTTTG CATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG CTGCCTCAAG TATCCAGAGA GTCTTGTCAA CATTAACACT AGCAGTATTT
    CCAACACTTT ATTTTTTTAA CTTCCTTTAT TATACAGAAG CAGGATCTAT GTTTTTTACT
    CTTTTTGCAT ATTTGATGTG TCTTTATGGA AATCATAAAA CTTCAGCCTT CCTTGGATTT
    TGTGGCTTCA TGTTTCGGCA AACAAATATC ATCTGGGCTG TCTTCTGTGC AGGGAATGTC
    ATTGCACAAA AGTTAACTGA GGCTTGGAAA ACTGAGCTAC AAAAGAAGGA AGACAGACTT
    CCACCTATTA AAGGACCATT TGCAGAATTC AGAAAAATTC TTCAGTTTCT TTTGGCTTAT
    TCCATGTCCT TTAAAAACTT GAGTATGCTT TTCTGTTTGA CTTGGCCCTA CATCCTTCTG
    GGATTTCTGT TTTGTGCTTT TGTAGTAGTT AATGGTGGAA TTGTTATTGG CGATCGGAGT
    AGTCATGAAG CCTGTCTTCA TTTTCCTCAA CTATTCTACT TTTTTTCATT TACTCTCTTT
    TTTTCTTTTC CTCATCTCCT GTCTCCTAGC AAAATTAAGA CTTTTCTTTC CTTAGTTTGG
    AAACATGGAA TTCTGTTTTT GGTGGTTACC TTAGTCTCTG TGTTTTTAGT TTGGAAATTC
    ACTTATGCTC ATAAATACTT GCTAGCAGAC AATAGACATT ATACTTTCTA TGTGTGGAAA
    AGAGTTTTTC AAAGATATGC AATTCTGAAA TATTTGTTAG TTCCAGCCTA TATATTTGCT
    GGTTGGAGTA TAGCTGACTC ATTGAAATCA AAGCCAATTT TTTGGAATTT AATGTTTTTC
    ATATGCTTGT TCATTGTTAT AGTTCCTCAG AAACTGCTGG AATTTCGTTA CTTCATTTTA
    CCTTATGTCA TTTATAGGCT TAACATAACT CTGCCTCCCA CATCCAGACT TGTTTGTGAA
    CTGAGTTGCT ATGCAATTGT TAATTTCATA ACTTTTTACA TCTTTCTGAA CAAGACTTTT
    CAGTGGCCAA ATAGTCAGGA CATTCAAAGG TTTATGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107245
    Clone ID Related Accession (Same CDS sequence) NM_001013620.4
    Accession Version NM_001013620.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117372.2. On Nov 23, 2018 this sequence version replaced NM_001013620.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.398127.1, SRR1163657.499250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308742.9/ ENSP00000310120.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG CATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG CTGCCTCAAG TATCCAGAGA GTCTTGTCAA CATTAACACT AGCAGTATTT
    CCAACACTTT ATTTTTTTAA CTTCCTTTAT TATACAGAAG CAGGATCTAT GTTTTTTACT
    CTTTTTGCAT ATTTGATGTG TCTTTATGGA AATCATAAAA CTTCAGCCTT CCTTGGATTT
    TGTGGCTTCA TGTTTCGGCA AACAAATATC ATCTGGGCTG TCTTCTGTGC AGGGAATGTC
    ATTGCACAAA AGTTAACTGA GGCTTGGAAA ACTGAGCTAC AAAAGAAGGA AGACAGACTT
    CCACCTATTA AAGGACCATT TGCAGAATTC AGAAAAATTC TTCAGTTTCT TTTGGCTTAT
    TCCATGTCCT TTAAAAACTT GAGTATGCTT TTCTGTTTGA CTTGGCCCTA CATCCTTCTG
    GGATTTCTGT TTTGTGCTTT TGTAGTAGTT AATGGTGGAA TTGTTATTGG CGATCGGAGT
    AGTCATGAAG CCTGTCTTCA TTTTCCTCAA CTATTCTACT TTTTTTCATT TACTCTCTTT
    TTTTCTTTTC CTCATCTCCT GTCTCCTAGC AAAATTAAGA CTTTTCTTTC CTTAGTTTGG
    AAACATGGAA TTCTGTTTTT GGTGGTTACC TTAGTCTCTG TGTTTTTAGT TTGGAAATTC
    ACTTATGCTC ATAAATACTT GCTAGCAGAC AATAGACATT ATACTTTCTA TGTGTGGAAA
    AGAGTTTTTC AAAGATATGC AATTCTGAAA TATTTGTTAG TTCCAGCCTA TATATTTGCT
    GGTTGGAGTA TAGCTGACTC ATTGAAATCA AAGCCAATTT TTTGGAATTT AATGTTTTTC
    ATATGCTTGT TCATTGTTAT AGTTCCTCAG AAACTGCTGG AATTTCGTTA CTTCATTTTA
    CCTTATGTCA TTTATAGGCT TAACATAACT CTGCCTCCCA CATCCAGACT TGTTTGTGAA
    CTGAGTTGCT ATGCAATTGT TAATTTCATA ACTTTTTACA TCTTTCTGAA CAAGACTTTT
    CAGTGGCCAA ATAGTCAGGA CATTCAAAGG TTTATGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001013642.2
    CDS121..1542
    Translation

    Target ORF information:

    RefSeq Version NM_001013620.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001013620.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG CATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG CTGCCTCAAG TATCCAGAGA GTCTTGTCAA CATTAACACT AGCAGTATTT
    CCAACACTTT ATTTTTTTAA CTTCCTTTAT TATACAGAAG CAGGATCTAT GTTTTTTACT
    CTTTTTGCAT ATTTGATGTG TCTTTATGGA AATCATAAAA CTTCAGCCTT CCTTGGATTT
    TGTGGCTTCA TGTTTCGGCA AACAAATATC ATCTGGGCTG TCTTCTGTGC AGGGAATGTC
    ATTGCACAAA AGTTAACTGA GGCTTGGAAA ACTGAGCTAC AAAAGAAGGA AGACAGACTT
    CCACCTATTA AAGGACCATT TGCAGAATTC AGAAAAATTC TTCAGTTTCT TTTGGCTTAT
    TCCATGTCCT TTAAAAACTT GAGTATGCTT TTCTGTTTGA CTTGGCCCTA CATCCTTCTG
    GGATTTCTGT TTTGTGCTTT TGTAGTAGTT AATGGTGGAA TTGTTATTGG CGATCGGAGT
    AGTCATGAAG CCTGTCTTCA TTTTCCTCAA CTATTCTACT TTTTTTCATT TACTCTCTTT
    TTTTCTTTTC CTCATCTCCT GTCTCCTAGC AAAATTAAGA CTTTTCTTTC CTTAGTTTGG
    AAACATGGAA TTCTGTTTTT GGTGGTTACC TTAGTCTCTG TGTTTTTAGT TTGGAAATTC
    ACTTATGCTC ATAAATACTT GCTAGCAGAC AATAGACATT ATACTTTCTA TGTGTGGAAA
    AGAGTTTTTC AAAGATATGC AATTCTGAAA TATTTGTTAG TTCCAGCCTA TATATTTGCT
    GGTTGGAGTA TAGCTGACTC ATTGAAATCA AAGCCAATTT TTTGGAATTT AATGTTTTTC
    ATATGCTTGT TCATTGTTAT AGTTCCTCAG AAACTGCTGG AATTTCGTTA CTTCATTTTA
    CCTTATGTCA TTTATAGGCT TAACATAACT CTGCCTCCCA CATCCAGACT TGTTTGTGAA
    CTGAGTTGCT ATGCAATTGT TAATTTCATA ACTTTTTACA TCTTTCTGAA CAAGACTTTT
    CAGTGGCCAA ATAGTCAGGA CATTCAAAGG TTTATGTGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108222
    Clone ID Related Accession (Same CDS sequence) NM_001308340.2
    Accession Version NM_001308340.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117372.2. On May 31, 2019 this sequence version replaced NM_001308340.1. Transcript Variant: This variant (2) uses an alternate splice site in its 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.78151.1, AL712776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG CATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG AATACTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001295269.2
    CDS121..501
    Translation

    Target ORF information:

    RefSeq Version NM_001308340.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ALG10 alpha-1,2-glucosyltransferase B (ALG10B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001308340.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGCGCAGC TAGAGGGTTA CTGTTTCTCG GCCGCCTTGA GCTGTACCTT TTTAGTGTCC 
    TGCCTCCTCT TCTCCGCCTT CAGCCGGGCG CTGCGAGAGC CCTACATGGA CGAGATCTTC
    CACCTGCCTC AGGCGCAGCG CTACTGTGAG GGCCATTTCT CCCTTTCCCA GTGGGATCCC
    ATGATTACTA CATTACCTGG CTTGTACCTG GTGTCAGTTG GAGTGGTCAA ACCTGCCATT
    TGGATCTTTG CATGGTCTGA ACATGTTGTC TGCTCCATTG GGATGCTCAG ATTTGTTAAT
    CTTCTCTTCA GTGTTGGCAA CTTCTATTTA CTATATTTGC TTTTCCACAA GGTACAACCC
    AGAAACAAGG AATACTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.