HECTD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECTD2
Entrez Gene ID	143279
Full Name	HECT domain E3 ubiquitin protein ligase 2
General protein information	Preferred Names HECT domain E3 ubiquitin protein ligase 2 Names probable E3 ubiquitin-protein ligase HECTD2 HECT domain containing E3 ubiquitin protein ligase 2 HECT domain-containing protein 2 HECT-type E3 ubiquitin transferase HECTD2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.32

mRNA and Protein(s)

mRNA	Protein	Name
XM_017015752.1	XP_016871241.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
NM_001284274.2	NP_001271203.1	probable E3 ubiquitin-protein ligase HECTD2 isoform c
XM_017015752.2	XP_016871241.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
NM_182765.5	NP_877497.3	probable E3 ubiquitin-protein ligase HECTD2 isoform a
NM_001348365.1	NP_001335294.1	probable E3 ubiquitin-protein ligase HECTD2 isoform d
NM_173497.3	NP_775768.3	probable E3 ubiquitin-protein ligase HECTD2 isoform b
XM_024447828.1	XP_024303596.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X1
XM_017015751.1	XP_016871240.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X2

Pathways from BioSystems

Homologies

	HECTD2	XP_001088322.1
Gallus gallus (chicken)	HECTD2	XP_004942207.1
Xenopus tropicalis (tropical clawed frog)	hectd2	NP_001106408.1
Pan troglodytes (chimpanzee)	HECTD2	XP_003312725.1
Homo sapiens (human)	HECTD2	NP_877497.3
Canis lupus familiaris (dog)	HECTD2	XP_543924.2
Bos taurus (cattle)	HECTD2	NP_001193701.1
Rattus norvegicus (Norway rat)	Hectd2	NP_001101078.2
Mus musculus (house mouse)	Hectd2	NP_001156943.1
Danio rerio (zebrafish)	hectd2	XP_691455.5

Gene Ontology
Bibliography

Related articles in PubMed

MiR-221 promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A.
Sun T, Wang X, He HH, Sweeney CJ, Liu SX, Brown M, Balk S, Lee GS, Kantoff PW
Oncogene33(21)2790-800(2014 May)

Absence of association between two HECTD2 polymorphisms and sporadic Creutzfeldt-Jakob disease.
Jeong BH, Lee KH, Lee YJ, Yun J, Park YJ, Cho HJ, Kim YH, Cho YS, Choi EK, Carp RI, Kim YS
Dementia and geriatric cognitive disorders31(2)146-51(2011)

HECTD2, a candidate susceptibility gene for Alzheimer's disease on 10q.
Lloyd SE, Rossor M, Fox N, Mead S, Collinge J
BMC medical genetics1090(2009 Sep)

Maturation of human dendritic cells is accompanied by functional remodelling of the ubiquitin-proteasome system.
Ebstein F, Lange N, Urban S, Seifert U, Kr?ger E, Kloetzel PM
The international journal of biochemistry & cell biology41(5)1205-15(2009 May)

HECTD2 is associated with susceptibility to mouse and human prion disease.
Lloyd SE, Maytham EG, Pota H, Grizenkova J, Molou E, Uphill J, Hummerich H, Whitfield J, Alpers MP, Mead S, Collinge J
PLoS genetics5(2)e1000383(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A major biological consequence of upregulation of miR-221 is reprogramming of androgen receptor signaling via downregulation of HECTD2 and RAB1A.
Title: MiR-221 promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A.

HECTD2 polymorphisms are not associated with genetic susceptibility to sporadic Creutzfeldt-Jakob disease in a Korean population
Title: Absence of association between two HECTD2 polymorphisms and sporadic Creutzfeldt-Jakob disease.

The common haplotypes of HECTD2, tagged by rs12249854, are not associated with susceptibility to late onset Alzheimer's disease
Title: HECTD2, a candidate susceptibility gene for Alzheimer's disease on 10q.

HECTD2 haplotypes are associated with the susceptibility to human prion diseases.
Title: HECTD2 is associated with susceptibility to mouse and human prion disease.

Observational study of gene-disease association. (HuGE Navigator)
Title: A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.

The following HECTD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07262	NM_173497.3 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu91143	XM_017015751.1 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu91144	XM_017015752.2 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu02301	NM_182765.5 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu05306	NM_001284274.2 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu101440	NM_001348365.1 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu101440	XM_024447828.1 Latest version!	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu91144	XM_017015752.1	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECTD2 ( NM_173497.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775768.3 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07262
Clone ID Related Accession (Same CDS sequence)	NM_173497.3
Accession Version	NM_173497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY158536.1, AK094625.1, BG900953.1 and AW021859.1. On Sep 27, 2013 this sequence version replaced NM_173497.2. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 3. The resulting isoform (b) has a distinct C-terminus and is shorter than isoform c. ##Evidence-Data-START## Transcript exon combination :: AK094625.1, SRR1660809.103192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGGCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
GTAAGTATTA TGACATGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775768.3
CDS	155..778
Translation	MSEAVRVPSPATPLVVAAAAPEERKGKESEREKLPPIVSAGAGATAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTVSIMTCK

Target ORF information:

RefSeq Version	NM_173497.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173497.3

ORF Insert Sequence:

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGGCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
GTAAGTATTA TGACATGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_017015751.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871240.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu91143
Clone ID Related Accession (Same CDS sequence)	XM_017015751.1
Accession Version	XM_017015751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGCCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
CCTCAAGACG TTCAGAAGAC AGTATTAAAG GGAATCATTA ACAGCCTGTT ACGAGAATGG 
AAAGGTCCAC GAACAAAAGA TGATCTTAGA GCATATTTTA TACTTTTACA GAATCCTCAG 
TTTAATAACA CATCTACGTA TGTCATCTAT GCTCACTTGC TACGACAGAT AGCTACCTTA 
GTGGAAGCTG ACCATCATTT CCTAGTTCAC TGGTTTAAAA AATTGTCCCA GAAGAGGTTC 
AAACAATTGG TAGAGAGATT GCTGCAATTT ATTTCTTTAC GCCTGTTTCC TGCAAAGCCT 
GAAGAATTTC CACCTATAAC AAAGTGTTCC TGGTGGATTC CATCAGCCGC TAAAGTGTTG 
GCTTTACTTA ATACTGCAAA CAATTTAGTT CACCCTCCCC TTATTCCTTA TACTGATTTC 
TATAATTCTA CATTGGATCA CATTGATCTC ATGGAAGAAT ATCATACTTG GCAAAACTTT 
GGAAACTCTC ACAGGTTTTC CTTCTGTCAG TACCCATTCG TTATTTCTGT AGCTGCAAAA 
AAAATCATTA TTCAGAGAGA CTCAGAGCAA CAGATGATAA ACATCGCAAG GAGAAGGACA 
AAAGATTTGA GTAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871240.1
CDS	101..1321
Translation	MSEAVRVPSPATPLVVAAPAPEERKGKESEREKLPPIVSAGAGATAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLSQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLVHPPLIPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARRRTKDLSNW

Target ORF information:

RefSeq Version	XM_017015751.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015751.1

ORF Insert Sequence:

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGCCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
CCTCAAGACG TTCAGAAGAC AGTATTAAAG GGAATCATTA ACAGCCTGTT ACGAGAATGG 
AAAGGTCCAC GAACAAAAGA TGATCTTAGA GCATATTTTA TACTTTTACA GAATCCTCAG 
TTTAATAACA CATCTACGTA TGTCATCTAT GCTCACTTGC TACGACAGAT AGCTACCTTA 
GTGGAAGCTG ACCATCATTT CCTAGTTCAC TGGTTTAAAA AATTGTCCCA GAAGAGGTTC 
AAACAATTGG TAGAGAGATT GCTGCAATTT ATTTCTTTAC GCCTGTTTCC TGCAAAGCCT 
GAAGAATTTC CACCTATAAC AAAGTGTTCC TGGTGGATTC CATCAGCCGC TAAAGTGTTG 
GCTTTACTTA ATACTGCAAA CAATTTAGTT CACCCTCCCC TTATTCCTTA TACTGATTTC 
TATAATTCTA CATTGGATCA CATTGATCTC ATGGAAGAAT ATCATACTTG GCAAAACTTT 
GGAAACTCTC ACAGGTTTTC CTTCTGTCAG TACCCATTCG TTATTTCTGT AGCTGCAAAA 
AAAATCATTA TTCAGAGAGA CTCAGAGCAA CAGATGATAA ACATCGCAAG GAGAAGGACA 
AAAGATTTGA GTAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_017015752.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871241.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu91144
Clone ID Related Accession (Same CDS sequence)	XM_017015752.1 , XM_017015752.2
Accession Version	XM_017015752.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT ATCATACTTG GCAAAACTTT GGAAACTCTC ACAGGTTTTC CTTCTGTCAG 
TACCCATTCG TTATTTCTGT AGCTGCAAAA AAAATCATTA TTCAGAGAGA CTCAGAGCAA 
CAGATGATAA ACATCGCAAG GCAAAGTCTG GTGGATAAAG TATCTCGAAG ACAGAGACCT 
GATATGAATA TATTATTTCT AAATATGAAA GTAAGACGGA CACATCTGGT TAGCGATTCA 
CTTGATGAGC TAACCCGGAA AAGAGCCGAT TTGAAAAAGA AGTTAAAAGT TACATTTGTA 
GGGGAAGCTG GTTTGGATAT GGGTGGTTTG ACTAAAGAAT GGTTCCTTCT TCTAATTCGC 
CAAATTTTTC ATCCAGATTA TGGCATGTTT ACATATCACA AGGATTCACA CTGCCATTGG 
TTTAGCAGCT TTAAATGTGA TAACTATTCT GAATTCCGAT TGGTTGGAAT TCTTATGGGA 
CTAGCTGTTT ATAACAGCAT CACCTTGGAT ATTCGTTTCC CTCCCTGCTG TTACAAGAAA 
TTATTGAGCC CTCCCATCAT TCCTAGTGAT CAAAATATAC CAGTAGGCAT CTGCAATGTT 
ACCGTGGACG ACTTATGTCA AATTATGCCT GAGTTGGCCC ATGGATTAAG TGAACTCTTA 
TCACATGAAG GCAATGTTGA AGAAGATTTC TATTCAACAT TTCAGGTTTT TCAAGAAGAA 
TTTGGAATAA TCAAGTCCTA TAATTTAAAG CCCGGTGGTG ATAAAATTTC AGTTACCAAT 
CAAAATAGAA AAGAATATGT ACAGCTTTAT ACTGACTTCC TTCTGAACAA ATCCATCTAT 
AAGCAGTTTG CTGCATTTTA TTATGGATTT CATAGTGTGT GTGCTTCAAA TGCCCTAATG 
CTGCTTCGTC CAGAAGAGGT TGAAATTTTG GTCTGTGGAA GTCCTGACCT GGATATGCAT 
GCTCTGCAGA GAAGTACTCA GTATGATGGC TATGCAAAAA CGGACTTAAC TATAAAATAC 
TTTTGGGATG TTGTGCTTGG ATTTCCTCTT GATCTTCAAA AGAAGTTGCT ACATTTTACT 
ACAGGGAGTG ACAGAGTACC TGTAGGAGGG ATGGCTGATT TGAACTTTAA AATCTCAAAG 
AATGAAACTT CTACTAACTG CTTACCTGTG GCCCATACCT GCTTCAATCA ACTTTGCCTT 
CCCCCCTACA AGAGCAAAAA GGATCTGAAA CAGAAATTGA TCATTGGAAT TTCAAATTCA 
GAAGGTTTTG GACTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871241.1
CDS	409..1689
Translation	MEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_017015752.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015752.1

ORF Insert Sequence:

ATGGAAGAAT ATCATACTTG GCAAAACTTT GGAAACTCTC ACAGGTTTTC CTTCTGTCAG 
TACCCATTCG TTATTTCTGT AGCTGCAAAA AAAATCATTA TTCAGAGAGA CTCAGAGCAA 
CAGATGATAA ACATCGCAAG GCAAAGTCTG GTGGATAAAG TATCTCGAAG ACAGAGACCT 
GATATGAATA TATTATTTCT AAATATGAAA GTAAGACGGA CACATCTGGT TAGCGATTCA 
CTTGATGAGC TAACCCGGAA AAGAGCCGAT TTGAAAAAGA AGTTAAAAGT TACATTTGTA 
GGGGAAGCTG GTTTGGATAT GGGTGGTTTG ACTAAAGAAT GGTTCCTTCT TCTAATTCGC 
CAAATTTTTC ATCCAGATTA TGGCATGTTT ACATATCACA AGGATTCACA CTGCCATTGG 
TTTAGCAGCT TTAAATGTGA TAACTATTCT GAATTCCGAT TGGTTGGAAT TCTTATGGGA 
CTAGCTGTTT ATAACAGCAT CACCTTGGAT ATTCGTTTCC CTCCCTGCTG TTACAAGAAA 
TTATTGAGCC CTCCCATCAT TCCTAGTGAT CAAAATATAC CAGTAGGCAT CTGCAATGTT 
ACCGTGGACG ACTTATGTCA AATTATGCCT GAGTTGGCCC ATGGATTAAG TGAACTCTTA 
TCACATGAAG GCAATGTTGA AGAAGATTTC TATTCAACAT TTCAGGTTTT TCAAGAAGAA 
TTTGGAATAA TCAAGTCCTA TAATTTAAAG CCCGGTGGTG ATAAAATTTC AGTTACCAAT 
CAAAATAGAA AAGAATATGT ACAGCTTTAT ACTGACTTCC TTCTGAACAA ATCCATCTAT 
AAGCAGTTTG CTGCATTTTA TTATGGATTT CATAGTGTGT GTGCTTCAAA TGCCCTAATG 
CTGCTTCGTC CAGAAGAGGT TGAAATTTTG GTCTGTGGAA GTCCTGACCT GGATATGCAT 
GCTCTGCAGA GAAGTACTCA GTATGATGGC TATGCAAAAA CGGACTTAAC TATAAAATAC 
TTTTGGGATG TTGTGCTTGG ATTTCCTCTT GATCTTCAAA AGAAGTTGCT ACATTTTACT 
ACAGGGAGTG ACAGAGTACC TGTAGGAGGG ATGGCTGATT TGAACTTTAA AATCTCAAAG 
AATGAAACTT CTACTAACTG CTTACCTGTG GCCCATACCT GCTTCAATCA ACTTTGCCTT 
CCCCCCTACA AGAGCAAAAA GGATCTGAAA CAGAAATTGA TCATTGGAAT TTCAAATTCA 
GAAGGTTTTG GACTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_017015752.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871241.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.

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CloneID	OHu91144
Clone ID Related Accession (Same CDS sequence)	XM_017015752.1 , XM_017015752.2
Accession Version	XM_017015752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017015752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT ATCATACTTG GCAAAACTTT GGAAACTCTC ACAGGTTTTC CTTCTGTCAG 
TACCCATTCG TTATTTCTGT AGCTGCAAAA AAAATCATTA TTCAGAGAGA CTCAGAGCAA 
CAGATGATAA ACATCGCAAG GCAAAGTCTG GTGGATAAAG TATCTCGAAG ACAGAGACCT 
GATATGAATA TATTATTTCT AAATATGAAA GTAAGACGGA CACATCTGGT TAGCGATTCA 
CTTGATGAGC TAACCCGGAA AAGAGCCGAT TTGAAAAAGA AGTTAAAAGT TACATTTGTA 
GGGGAAGCTG GTTTGGATAT GGGTGGTTTG ACTAAAGAAT GGTTCCTTCT TCTAATTCGC 
CAAATTTTTC ATCCAGATTA TGGCATGTTT ACATATCACA AGGATTCACA CTGCCATTGG 
TTTAGCAGCT TTAAATGTGA TAACTATTCT GAATTCCGAT TGGTTGGAAT TCTTATGGGA 
CTAGCTGTTT ATAACAGCAT CACCTTGGAT ATTCGTTTCC CTCCCTGCTG TTACAAGAAA 
TTATTGAGCC CTCCCATCAT TCCTAGTGAT CAAAATATAC CAGTAGGCAT CTGCAATGTT 
ACCGTGGACG ACTTATGTCA AATTATGCCT GAGTTGGCCC ATGGATTAAG TGAACTCTTA 
TCACATGAAG GCAATGTTGA AGAAGATTTC TATTCAACAT TTCAGGTTTT TCAAGAAGAA 
TTTGGAATAA TCAAGTCCTA TAATTTAAAG CCCGGTGGTG ATAAAATTTC AGTTACCAAT 
CAAAATAGAA AAGAATATGT ACAGCTTTAT ACTGACTTCC TTCTGAACAA ATCCATCTAT 
AAGCAGTTTG CTGCATTTTA TTATGGATTT CATAGTGTGT GTGCTTCAAA TGCCCTAATG 
CTGCTTCGTC CAGAAGAGGT TGAAATTTTG GTCTGTGGAA GTCCTGACCT GGATATGCAT 
GCTCTGCAGA GAAGTACTCA GTATGATGGC TATGCAAAAA CGGACTTAAC TATAAAATAC 
TTTTGGGATG TTGTGCTTGG ATTTCCTCTT GATCTTCAAA AGAAGTTGCT ACATTTTACT 
ACAGGGAGTG ACAGAGTACC TGTAGGAGGG ATGGCTGATT TGAACTTTAA AATCTCAAAG 
AATGAAACTT CTACTAACTG CTTACCTGTG GCCCATACCT GCTTCAATCA ACTTTGCCTT 
CCCCCCTACA AGAGCAAAAA GGATCTGAAA CAGAAATTGA TCATTGGAAT TTCAAATTCA 
GAAGGTTTTG GACTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871241.1
CDS	407..1687
Translation	MEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_017015752.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015752.2

ORF Insert Sequence:

ATGGAAGAAT ATCATACTTG GCAAAACTTT GGAAACTCTC ACAGGTTTTC CTTCTGTCAG 
TACCCATTCG TTATTTCTGT AGCTGCAAAA AAAATCATTA TTCAGAGAGA CTCAGAGCAA 
CAGATGATAA ACATCGCAAG GCAAAGTCTG GTGGATAAAG TATCTCGAAG ACAGAGACCT 
GATATGAATA TATTATTTCT AAATATGAAA GTAAGACGGA CACATCTGGT TAGCGATTCA 
CTTGATGAGC TAACCCGGAA AAGAGCCGAT TTGAAAAAGA AGTTAAAAGT TACATTTGTA 
GGGGAAGCTG GTTTGGATAT GGGTGGTTTG ACTAAAGAAT GGTTCCTTCT TCTAATTCGC 
CAAATTTTTC ATCCAGATTA TGGCATGTTT ACATATCACA AGGATTCACA CTGCCATTGG 
TTTAGCAGCT TTAAATGTGA TAACTATTCT GAATTCCGAT TGGTTGGAAT TCTTATGGGA 
CTAGCTGTTT ATAACAGCAT CACCTTGGAT ATTCGTTTCC CTCCCTGCTG TTACAAGAAA 
TTATTGAGCC CTCCCATCAT TCCTAGTGAT CAAAATATAC CAGTAGGCAT CTGCAATGTT 
ACCGTGGACG ACTTATGTCA AATTATGCCT GAGTTGGCCC ATGGATTAAG TGAACTCTTA 
TCACATGAAG GCAATGTTGA AGAAGATTTC TATTCAACAT TTCAGGTTTT TCAAGAAGAA 
TTTGGAATAA TCAAGTCCTA TAATTTAAAG CCCGGTGGTG ATAAAATTTC AGTTACCAAT 
CAAAATAGAA AAGAATATGT ACAGCTTTAT ACTGACTTCC TTCTGAACAA ATCCATCTAT 
AAGCAGTTTG CTGCATTTTA TTATGGATTT CATAGTGTGT GTGCTTCAAA TGCCCTAATG 
CTGCTTCGTC CAGAAGAGGT TGAAATTTTG GTCTGTGGAA GTCCTGACCT GGATATGCAT 
GCTCTGCAGA GAAGTACTCA GTATGATGGC TATGCAAAAA CGGACTTAAC TATAAAATAC 
TTTTGGGATG TTGTGCTTGG ATTTCCTCTT GATCTTCAAA AGAAGTTGCT ACATTTTACT 
ACAGGGAGTG ACAGAGTACC TGTAGGAGGG ATGGCTGATT TGAACTTTAA AATCTCAAAG 
AATGAAACTT CTACTAACTG CTTACCTGTG GCCCATACCT GCTTCAATCA ACTTTGCCTT 
CCCCCCTACA AGAGCAAAAA GGATCTGAAA CAGAAATTGA TCATTGGAAT TTCAAATTCA 
GAAGGTTTTG GACTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( NM_182765.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_877497.3 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 1, mRNA.

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CloneID	OHu02301
Clone ID Related Accession (Same CDS sequence)	NM_182765.5
Accession Version	NM_182765.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY158536.1, AK094625.1, BC040187.1, AK122629.1 and BC036487.1. On Jan 28, 2017 this sequence version replaced NM_182765.4. Transcript Variant: This variant (1) lacks an alternate in-frame exon in the 5' coding region compared to variant 3. It encodes isoform a which is shorter compared to isoform c. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.107025.1, BC040187.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGGCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
CCTCAAGACG TTCAGAAGAC AGTATTAAAG GGAATCATTA ACAGCCTGTT ACGAGAATGG 
AAAGGTCCAC GAACAAAAGA TGATCTTAGA GCATATTTTA TACTTTTACA GAATCCTCAG 
TTTAATAACA CATCTACGTA TGTCATCTAT GCTCACTTGC TACGACAGAT AGCTACCTTA 
GTGGAAGCTG ACCATCATTT CCTAGTTCAC TGGTTTAAAA AATTGTCCCA GAAGAGGTTC 
AAACAATTGG TAGAGAGATT GCTGCAATTT ATTTCTTTAC GCCTGTTTCC TGCAAAGCCT 
GAAGAATTTC CACCTATAAC AAAGTGTTCC TGGTGGATTC CATCAGCCGC TAAAGTGTTG 
GCTTTACTTA ATACTGCAAA CAATTTAGTT CACCCTCCCC TTATTCCTTA TACTGATTTC 
TATAATTCTA CATTGGATCA CATTGATCTC ATGGAAGAAT ATCATACTTG GCAAAACTTT 
GGAAACTCTC ACAGGTTTTC CTTCTGTCAG TACCCATTCG TTATTTCTGT AGCTGCAAAA 
AAAATCATTA TTCAGAGAGA CTCAGAGCAA CAGATGATAA ACATCGCAAG GCAAAGTCTG 
GTGGATAAAG TATCTCGAAG ACAGAGACCT GATATGAATA TATTATTTCT AAATATGAAA 
GTAAGACGGA CACATCTGGT TAGCGATTCA CTTGATGAGC TAACCCGGAA AAGAGCCGAT 
TTGAAAAAGA AGTTAAAAGT TACATTTGTA GGGGAAGCTG GTTTGGATAT GGGTGGTTTG 
ACTAAAGAAT GGTTCCTTCT TCTAATTCGC CAAATTTTTC ATCCAGATTA TGGCATGTTT 
ACATATCACA AGGATTCACA CTGCCATTGG TTTAGCAGCT TTAAATGTGA TAACTATTCT 
GAATTCCGAT TGGTTGGAAT TCTTATGGGA CTAGCTGTTT ATAACAGCAT CACCTTGGAT 
ATTCGTTTCC CTCCCTGCTG TTACAAGAAA TTATTGAGCC CTCCCATCAT TCCTAGTGAT 
CAAAATATAC CAGTAGGCAT CTGCAATGTT ACCGTGGACG ACTTATGTCA AATTATGCCT 
GAGTTGGCCC ATGGATTAAG TGAACTCTTA TCACATGAAG GCAATGTTGA AGAAGATTTC 
TATTCAACAT TTCAGGTTTT TCAAGAAGAA TTTGGAATAA TCAAGTCCTA TAATTTAAAG 
CCCGGTGGTG ATAAAATTTC AGTTACCAAT CAAAATAGAA AAGAATATGT ACAGCTTTAT 
ACTGACTTCC TTCTGAACAA ATCCATCTAT AAGCAGTTTG CTGCATTTTA TTATGGATTT 
CATAGTGTGT GTGCTTCAAA TGCCCTAATG CTGCTTCGTC CAGAAGAGGT TGAAATTTTG 
GTCTGTGGAA GTCCTGACCT GGATATGCAT GCTCTGCAGA GAAGTACTCA GTATGATGGC 
TATGCAAAAA CGGACTTAAC TATAAAATAC TTTTGGGATG TTGTGCTTGG ATTTCCTCTT 
GATCTTCAAA AGAAGTTGCT ACATTTTACT ACAGGGAGTG ACAGAGTACC TGTAGGAGGG 
ATGGCTGATT TGAACTTTAA AATCTCAAAG AATGAAACTT CTACTAACTG CTTACCTGTG 
GCCCATACCT GCTTCAATCA ACTTTGCCTT CCCCCCTACA AGAGCAAAAA GGATCTGAAA 
CAGAAATTGA TCATTGGAAT TTCAAATTCA GAAGGTTTTG GACTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877497.3
CDS	155..2485
Translation	MSEAVRVPSPATPLVVAAAAPEERKGKESEREKLPPIVSAGAGATAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLSQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLVHPPLIPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	NM_182765.5
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182765.5

ORF Insert Sequence:

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGGCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
CCTCAAGACG TTCAGAAGAC AGTATTAAAG GGAATCATTA ACAGCCTGTT ACGAGAATGG 
AAAGGTCCAC GAACAAAAGA TGATCTTAGA GCATATTTTA TACTTTTACA GAATCCTCAG 
TTTAATAACA CATCTACGTA TGTCATCTAT GCTCACTTGC TACGACAGAT AGCTACCTTA 
GTGGAAGCTG ACCATCATTT CCTAGTTCAC TGGTTTAAAA AATTGTCCCA GAAGAGGTTC 
AAACAATTGG TAGAGAGATT GCTGCAATTT ATTTCTTTAC GCCTGTTTCC TGCAAAGCCT 
GAAGAATTTC CACCTATAAC AAAGTGTTCC TGGTGGATTC CATCAGCCGC TAAAGTGTTG 
GCTTTACTTA ATACTGCAAA CAATTTAGTT CACCCTCCCC TTATTCCTTA TACTGATTTC 
TATAATTCTA CATTGGATCA CATTGATCTC ATGGAAGAAT ATCATACTTG GCAAAACTTT 
GGAAACTCTC ACAGGTTTTC CTTCTGTCAG TACCCATTCG TTATTTCTGT AGCTGCAAAA 
AAAATCATTA TTCAGAGAGA CTCAGAGCAA CAGATGATAA ACATCGCAAG GCAAAGTCTG 
GTGGATAAAG TATCTCGAAG ACAGAGACCT GATATGAATA TATTATTTCT AAATATGAAA 
GTAAGACGGA CACATCTGGT TAGCGATTCA CTTGATGAGC TAACCCGGAA AAGAGCCGAT 
TTGAAAAAGA AGTTAAAAGT TACATTTGTA GGGGAAGCTG GTTTGGATAT GGGTGGTTTG 
ACTAAAGAAT GGTTCCTTCT TCTAATTCGC CAAATTTTTC ATCCAGATTA TGGCATGTTT 
ACATATCACA AGGATTCACA CTGCCATTGG TTTAGCAGCT TTAAATGTGA TAACTATTCT 
GAATTCCGAT TGGTTGGAAT TCTTATGGGA CTAGCTGTTT ATAACAGCAT CACCTTGGAT 
ATTCGTTTCC CTCCCTGCTG TTACAAGAAA TTATTGAGCC CTCCCATCAT TCCTAGTGAT 
CAAAATATAC CAGTAGGCAT CTGCAATGTT ACCGTGGACG ACTTATGTCA AATTATGCCT 
GAGTTGGCCC ATGGATTAAG TGAACTCTTA TCACATGAAG GCAATGTTGA AGAAGATTTC 
TATTCAACAT TTCAGGTTTT TCAAGAAGAA TTTGGAATAA TCAAGTCCTA TAATTTAAAG 
CCCGGTGGTG ATAAAATTTC AGTTACCAAT CAAAATAGAA AAGAATATGT ACAGCTTTAT 
ACTGACTTCC TTCTGAACAA ATCCATCTAT AAGCAGTTTG CTGCATTTTA TTATGGATTT 
CATAGTGTGT GTGCTTCAAA TGCCCTAATG CTGCTTCGTC CAGAAGAGGT TGAAATTTTG 
GTCTGTGGAA GTCCTGACCT GGATATGCAT GCTCTGCAGA GAAGTACTCA GTATGATGGC 
TATGCAAAAA CGGACTTAAC TATAAAATAC TTTTGGGATG TTGTGCTTGG ATTTCCTCTT 
GATCTTCAAA AGAAGTTGCT ACATTTTACT ACAGGGAGTG ACAGAGTACC TGTAGGAGGG 
ATGGCTGATT TGAACTTTAA AATCTCAAAG AATGAAACTT CTACTAACTG CTTACCTGTG 
GCCCATACCT GCTTCAATCA ACTTTGCCTT CCCCCCTACA AGAGCAAAAA GGATCTGAAA 
CAGAAATTGA TCATTGGAAT TTCAAATTCA GAAGGTTTTG GACTTGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( NM_001284274.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271203.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 3, mRNA.

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CloneID	OHu05306
Clone ID Related Accession (Same CDS sequence)	NM_001284274.2
Accession Version	NM_001284274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY158536.1, AK295723.1, BC040187.1 and BC036487.1. On Jan 28, 2017 this sequence version replaced NM_001284274.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c). ##Evidence-Data-START## Transcript exon combination :: AK295723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGGCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
CCTCAAGACG TTCAGAAGAC AGTATTAAAG GGAATCATTA ACAGCCTGTT ACGAGAATGG 
AAAGGTCCAC GAACAAAAGA TGATCTTAGA GCATATTTTA TACTTTTACA GAATCCTCAG 
TTTAATAACA CATCTACGTA TGTCATCTAT GCTCACTTGC TACGACAGAT AGCTACCTTA 
GTGGAAGCTG ACCATCATTT CCTAGTTCAC TGGTTTAAAA AACAGTATTC AAGATTGTCC 
CAGAAGAGGT TCAAACAATT GGTAGAGAGA TTGCTGCAAT TTATTTCTTT ACGCCTGTTT 
CCTGCAAAGC CTGAAGAATT TCCACCTATA ACAAAGTGTT CCTGGTGGAT TCCATCAGCC 
GCTAAAGTGT TGGCTTTACT TAATACTGCA AACAATTTAG TTCACCCTCC CCTTATTCCT 
TATACTGATT TCTATAATTC TACATTGGAT CACATTGATC TCATGGAAGA ATATCATACT 
TGGCAAAACT TTGGAAACTC TCACAGGTTT TCCTTCTGTC AGTACCCATT CGTTATTTCT 
GTAGCTGCAA AAAAAATCAT TATTCAGAGA GACTCAGAGC AACAGATGAT AAACATCGCA 
AGGCAAAGTC TGGTGGATAA AGTATCTCGA AGACAGAGAC CTGATATGAA TATATTATTT 
CTAAATATGA AAGTAAGACG GACACATCTG GTTAGCGATT CACTTGATGA GCTAACCCGG 
AAAAGAGCCG ATTTGAAAAA GAAGTTAAAA GTTACATTTG TAGGGGAAGC TGGTTTGGAT 
ATGGGTGGTT TGACTAAAGA ATGGTTCCTT CTTCTAATTC GCCAAATTTT TCATCCAGAT 
TATGGCATGT TTACATATCA CAAGGATTCA CACTGCCATT GGTTTAGCAG CTTTAAATGT 
GATAACTATT CTGAATTCCG ATTGGTTGGA ATTCTTATGG GACTAGCTGT TTATAACAGC 
ATCACCTTGG ATATTCGTTT CCCTCCCTGC TGTTACAAGA AATTATTGAG CCCTCCCATC 
ATTCCTAGTG ATCAAAATAT ACCAGTAGGC ATCTGCAATG TTACCGTGGA CGACTTATGT 
CAAATTATGC CTGAGTTGGC CCATGGATTA AGTGAACTCT TATCACATGA AGGCAATGTT 
GAAGAAGATT TCTATTCAAC ATTTCAGGTT TTTCAAGAAG AATTTGGAAT AATCAAGTCC 
TATAATTTAA AGCCCGGTGG TGATAAAATT TCAGTTACCA ATCAAAATAG AAAAGAATAT 
GTACAGCTTT ATACTGACTT CCTTCTGAAC AAATCCATCT ATAAGCAGTT TGCTGCATTT 
TATTATGGAT TTCATAGTGT GTGTGCTTCA AATGCCCTAA TGCTGCTTCG TCCAGAAGAG 
GTTGAAATTT TGGTCTGTGG AAGTCCTGAC CTGGATATGC ATGCTCTGCA GAGAAGTACT 
CAGTATGATG GCTATGCAAA AACGGACTTA ACTATAAAAT ACTTTTGGGA TGTTGTGCTT 
GGATTTCCTC TTGATCTTCA AAAGAAGTTG CTACATTTTA CTACAGGGAG TGACAGAGTA 
CCTGTAGGAG GGATGGCTGA TTTGAACTTT AAAATCTCAA AGAATGAAAC TTCTACTAAC 
TGCTTACCTG TGGCCCATAC CTGCTTCAAT CAACTTTGCC TTCCCCCCTA CAAGAGCAAA 
AAGGATCTGA AACAGAAATT GATCATTGGA ATTTCAAATT CAGAAGGTTT TGGACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271203.1
CDS	155..2497
Translation	MSEAVRVPSPATPLVVAAAAPEERKGKESEREKLPPIVSAGAGATAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKQYSRLSQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLVHPPLIPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	NM_001284274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284274.2

ORF Insert Sequence:

ATGAGTGAGG CGGTTCGGGT ACCCTCGCCC GCCACTCCGC TGGTGGTGGC GGCGGCCGCG 
CCTGAGGAGA GGAAAGGGAA GGAGTCAGAG CGCGAGAAGC TGCCGCCCAT CGTATCGGCG 
GGCGCCGGCG CGACCGCGGG TTTGGACAGA GGAGCCAAAG GCCAAATTTC CACTTTCAGC 
AGTTTTATTT CAGCTGTTAG CCCGAAGAAA GAAGCTGCTG AAAACAGAAG TTCACCTGCA 
CATCTTGTTT TCCCTAACAT CAAGAATGTG AGAGAACCAC CACCAATTTG CCTTGATGTT 
AGACAAAAAC AGCGTACATC TATGGATGCA TCATCATCCG AAATGAAGGC CCCAGTCCTT 
CCAGAACCTA TTCTTCCTAT CCAGCCCAAA ACTGTGAAAG ACTTTCAGGA AGATGTAGAA 
AAAGTTAAGT CATCAGGAGA TTGGAAAGCA GTACATGATT TTTATCTAAC AACGTTTGAT 
TCTTTCCCAG AATTAAATGC TGCATTTAAG AAAGATGCCA CTGCCTCATT TAACACCATT 
GAAGACTCTG GGATTAATGC TAAATTTGTG AATGCTGTGT ATGATACCTT ACTTAATACT 
CCTCAAGACG TTCAGAAGAC AGTATTAAAG GGAATCATTA ACAGCCTGTT ACGAGAATGG 
AAAGGTCCAC GAACAAAAGA TGATCTTAGA GCATATTTTA TACTTTTACA GAATCCTCAG 
TTTAATAACA CATCTACGTA TGTCATCTAT GCTCACTTGC TACGACAGAT AGCTACCTTA 
GTGGAAGCTG ACCATCATTT CCTAGTTCAC TGGTTTAAAA AACAGTATTC AAGATTGTCC 
CAGAAGAGGT TCAAACAATT GGTAGAGAGA TTGCTGCAAT TTATTTCTTT ACGCCTGTTT 
CCTGCAAAGC CTGAAGAATT TCCACCTATA ACAAAGTGTT CCTGGTGGAT TCCATCAGCC 
GCTAAAGTGT TGGCTTTACT TAATACTGCA AACAATTTAG TTCACCCTCC CCTTATTCCT 
TATACTGATT TCTATAATTC TACATTGGAT CACATTGATC TCATGGAAGA ATATCATACT 
TGGCAAAACT TTGGAAACTC TCACAGGTTT TCCTTCTGTC AGTACCCATT CGTTATTTCT 
GTAGCTGCAA AAAAAATCAT TATTCAGAGA GACTCAGAGC AACAGATGAT AAACATCGCA 
AGGCAAAGTC TGGTGGATAA AGTATCTCGA AGACAGAGAC CTGATATGAA TATATTATTT 
CTAAATATGA AAGTAAGACG GACACATCTG GTTAGCGATT CACTTGATGA GCTAACCCGG 
AAAAGAGCCG ATTTGAAAAA GAAGTTAAAA GTTACATTTG TAGGGGAAGC TGGTTTGGAT 
ATGGGTGGTT TGACTAAAGA ATGGTTCCTT CTTCTAATTC GCCAAATTTT TCATCCAGAT 
TATGGCATGT TTACATATCA CAAGGATTCA CACTGCCATT GGTTTAGCAG CTTTAAATGT 
GATAACTATT CTGAATTCCG ATTGGTTGGA ATTCTTATGG GACTAGCTGT TTATAACAGC 
ATCACCTTGG ATATTCGTTT CCCTCCCTGC TGTTACAAGA AATTATTGAG CCCTCCCATC 
ATTCCTAGTG ATCAAAATAT ACCAGTAGGC ATCTGCAATG TTACCGTGGA CGACTTATGT 
CAAATTATGC CTGAGTTGGC CCATGGATTA AGTGAACTCT TATCACATGA AGGCAATGTT 
GAAGAAGATT TCTATTCAAC ATTTCAGGTT TTTCAAGAAG AATTTGGAAT AATCAAGTCC 
TATAATTTAA AGCCCGGTGG TGATAAAATT TCAGTTACCA ATCAAAATAG AAAAGAATAT 
GTACAGCTTT ATACTGACTT CCTTCTGAAC AAATCCATCT ATAAGCAGTT TGCTGCATTT 
TATTATGGAT TTCATAGTGT GTGTGCTTCA AATGCCCTAA TGCTGCTTCG TCCAGAAGAG 
GTTGAAATTT TGGTCTGTGG AAGTCCTGAC CTGGATATGC ATGCTCTGCA GAGAAGTACT 
CAGTATGATG GCTATGCAAA AACGGACTTA ACTATAAAAT ACTTTTGGGA TGTTGTGCTT 
GGATTTCCTC TTGATCTTCA AAAGAAGTTG CTACATTTTA CTACAGGGAG TGACAGAGTA 
CCTGTAGGAG GGATGGCTGA TTTGAACTTT AAAATCTCAA AGAATGAAAC TTCTACTAAC 
TGCTTACCTG TGGCCCATAC CTGCTTCAAT CAACTTTGCC TTCCCCCCTA CAAGAGCAAA 
AAGGATCTGA AACAGAAATT GATCATTGGA ATTTCAAATT CAGAAGGTTT TGGACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( NM_001348365.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335294.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu101440
Clone ID Related Accession (Same CDS sequence)	NM_001348365.1 , XM_024447828.1
Accession Version	NM_001348365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC023902.7, AL161798.23 and BC036487.1. On Jan 28, 2017 this sequence version replaced XM_017015750.1. Transcript Variant: This variant (5) uses an alternate 5' terminal exon and lacks an alternate in-frame exon in the 5' coding region compared to variant 3. These differences result in the use of a downstream start codon compared to variant 3. The encoded isoform (d) has a shorter N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.245576.1, SRR1803616.145507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAT CATCATCCGA AATGAAGGCC CCAGTCCTTC CAGAACCTAT TCTTCCTATC 
CAGCCCAAAA CTGTGAAAGA CTTTCAGGAA GATGTAGAAA AAGTTAAGTC ATCAGGAGAT 
TGGAAAGCAG TACATGATTT TTATCTAACA ACGTTTGATT CTTTCCCAGA ATTAAATGCT 
GCATTTAAGA AAGATGCCAC TGCCTCATTT AACACCATTG AAGACTCTGG GATTAATGCT 
AAATTTGTGA ATGCTGTGTA TGATACCTTA CTTAATACTC CTCAAGACGT TCAGAAGACA 
GTATTAAAGG GAATCATTAA CAGCCTGTTA CGAGAATGGA AAGGTCCACG AACAAAAGAT 
GATCTTAGAG CATATTTTAT ACTTTTACAG AATCCTCAGT TTAATAACAC ATCTACGTAT 
GTCATCTATG CTCACTTGCT ACGACAGATA GCTACCTTAG TGGAAGCTGA CCATCATTTC 
CTAGTTCACT GGTTTAAAAA ATTGTCCCAG AAGAGGTTCA AACAATTGGT AGAGAGATTG 
CTGCAATTTA TTTCTTTACG CCTGTTTCCT GCAAAGCCTG AAGAATTTCC ACCTATAACA 
AAGTGTTCCT GGTGGATTCC ATCAGCCGCT AAAGTGTTGG CTTTACTTAA TACTGCAAAC 
AATTTAGTTC ACCCTCCCCT TATTCCTTAT ACTGATTTCT ATAATTCTAC ATTGGATCAC 
ATTGATCTCA TGGAAGAATA TCATACTTGG CAAAACTTTG GAAACTCTCA CAGGTTTTCC 
TTCTGTCAGT ACCCATTCGT TATTTCTGTA GCTGCAAAAA AAATCATTAT TCAGAGAGAC 
TCAGAGCAAC AGATGATAAA CATCGCAAGG CAAAGTCTGG TGGATAAAGT ATCTCGAAGA 
CAGAGACCTG ATATGAATAT ATTATTTCTA AATATGAAAG TAAGACGGAC ACATCTGGTT 
AGCGATTCAC TTGATGAGCT AACCCGGAAA AGAGCCGATT TGAAAAAGAA GTTAAAAGTT 
ACATTTGTAG GGGAAGCTGG TTTGGATATG GGTGGTTTGA CTAAAGAATG GTTCCTTCTT 
CTAATTCGCC AAATTTTTCA TCCAGATTAT GGCATGTTTA CATATCACAA GGATTCACAC 
TGCCATTGGT TTAGCAGCTT TAAATGTGAT AACTATTCTG AATTCCGATT GGTTGGAATT 
CTTATGGGAC TAGCTGTTTA TAACAGCATC ACCTTGGATA TTCGTTTCCC TCCCTGCTGT 
TACAAGAAAT TATTGAGCCC TCCCATCATT CCTAGTGATC AAAATATACC AGTAGGCATC 
TGCAATGTTA CCGTGGACGA CTTATGTCAA ATTATGCCTG AGTTGGCCCA TGGATTAAGT 
GAACTCTTAT CACATGAAGG CAATGTTGAA GAAGATTTCT ATTCAACATT TCAGGTTTTT 
CAAGAAGAAT TTGGAATAAT CAAGTCCTAT AATTTAAAGC CCGGTGGTGA TAAAATTTCA 
GTTACCAATC AAAATAGAAA AGAATATGTA CAGCTTTATA CTGACTTCCT TCTGAACAAA 
TCCATCTATA AGCAGTTTGC TGCATTTTAT TATGGATTTC ATAGTGTGTG TGCTTCAAAT 
GCCCTAATGC TGCTTCGTCC AGAAGAGGTT GAAATTTTGG TCTGTGGAAG TCCTGACCTG 
GATATGCATG CTCTGCAGAG AAGTACTCAG TATGATGGCT ATGCAAAAAC GGACTTAACT 
ATAAAATACT TTTGGGATGT TGTGCTTGGA TTTCCTCTTG ATCTTCAAAA GAAGTTGCTA 
CATTTTACTA CAGGGAGTGA CAGAGTACCT GTAGGAGGGA TGGCTGATTT GAACTTTAAA 
ATCTCAAAGA ATGAAACTTC TACTAACTGC TTACCTGTGG CCCATACCTG CTTCAATCAA 
CTTTGCCTTC CCCCCTACAA GAGCAAAAAG GATCTGAAAC AGAAATTGAT CATTGGAATT 
TCAAATTCAG AAGGTTTTGG ACTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335294.1
CDS	371..2380
Translation	MDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLSQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLVHPPLIPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	NM_001348365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348365.1

ORF Insert Sequence:

ATGGATGCAT CATCATCCGA AATGAAGGCC CCAGTCCTTC CAGAACCTAT TCTTCCTATC 
CAGCCCAAAA CTGTGAAAGA CTTTCAGGAA GATGTAGAAA AAGTTAAGTC ATCAGGAGAT 
TGGAAAGCAG TACATGATTT TTATCTAACA ACGTTTGATT CTTTCCCAGA ATTAAATGCT 
GCATTTAAGA AAGATGCCAC TGCCTCATTT AACACCATTG AAGACTCTGG GATTAATGCT 
AAATTTGTGA ATGCTGTGTA TGATACCTTA CTTAATACTC CTCAAGACGT TCAGAAGACA 
GTATTAAAGG GAATCATTAA CAGCCTGTTA CGAGAATGGA AAGGTCCACG AACAAAAGAT 
GATCTTAGAG CATATTTTAT ACTTTTACAG AATCCTCAGT TTAATAACAC ATCTACGTAT 
GTCATCTATG CTCACTTGCT ACGACAGATA GCTACCTTAG TGGAAGCTGA CCATCATTTC 
CTAGTTCACT GGTTTAAAAA ATTGTCCCAG AAGAGGTTCA AACAATTGGT AGAGAGATTG 
CTGCAATTTA TTTCTTTACG CCTGTTTCCT GCAAAGCCTG AAGAATTTCC ACCTATAACA 
AAGTGTTCCT GGTGGATTCC ATCAGCCGCT AAAGTGTTGG CTTTACTTAA TACTGCAAAC 
AATTTAGTTC ACCCTCCCCT TATTCCTTAT ACTGATTTCT ATAATTCTAC ATTGGATCAC 
ATTGATCTCA TGGAAGAATA TCATACTTGG CAAAACTTTG GAAACTCTCA CAGGTTTTCC 
TTCTGTCAGT ACCCATTCGT TATTTCTGTA GCTGCAAAAA AAATCATTAT TCAGAGAGAC 
TCAGAGCAAC AGATGATAAA CATCGCAAGG CAAAGTCTGG TGGATAAAGT ATCTCGAAGA 
CAGAGACCTG ATATGAATAT ATTATTTCTA AATATGAAAG TAAGACGGAC ACATCTGGTT 
AGCGATTCAC TTGATGAGCT AACCCGGAAA AGAGCCGATT TGAAAAAGAA GTTAAAAGTT 
ACATTTGTAG GGGAAGCTGG TTTGGATATG GGTGGTTTGA CTAAAGAATG GTTCCTTCTT 
CTAATTCGCC AAATTTTTCA TCCAGATTAT GGCATGTTTA CATATCACAA GGATTCACAC 
TGCCATTGGT TTAGCAGCTT TAAATGTGAT AACTATTCTG AATTCCGATT GGTTGGAATT 
CTTATGGGAC TAGCTGTTTA TAACAGCATC ACCTTGGATA TTCGTTTCCC TCCCTGCTGT 
TACAAGAAAT TATTGAGCCC TCCCATCATT CCTAGTGATC AAAATATACC AGTAGGCATC 
TGCAATGTTA CCGTGGACGA CTTATGTCAA ATTATGCCTG AGTTGGCCCA TGGATTAAGT 
GAACTCTTAT CACATGAAGG CAATGTTGAA GAAGATTTCT ATTCAACATT TCAGGTTTTT 
CAAGAAGAAT TTGGAATAAT CAAGTCCTAT AATTTAAAGC CCGGTGGTGA TAAAATTTCA 
GTTACCAATC AAAATAGAAA AGAATATGTA CAGCTTTATA CTGACTTCCT TCTGAACAAA 
TCCATCTATA AGCAGTTTGC TGCATTTTAT TATGGATTTC ATAGTGTGTG TGCTTCAAAT 
GCCCTAATGC TGCTTCGTCC AGAAGAGGTT GAAATTTTGG TCTGTGGAAG TCCTGACCTG 
GATATGCATG CTCTGCAGAG AAGTACTCAG TATGATGGCT ATGCAAAAAC GGACTTAACT 
ATAAAATACT TTTGGGATGT TGTGCTTGGA TTTCCTCTTG ATCTTCAAAA GAAGTTGCTA 
CATTTTACTA CAGGGAGTGA CAGAGTACCT GTAGGAGGGA TGGCTGATTT GAACTTTAAA 
ATCTCAAAGA ATGAAACTTC TACTAACTGC TTACCTGTGG CCCATACCTG CTTCAATCAA 
CTTTGCCTTC CCCCCTACAA GAGCAAAAAG GATCTGAAAC AGAAATTGAT CATTGGAATT 
TCAAATTCAG AAGGTTTTGG ACTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_024447828.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303596.1 Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu101440
Clone ID Related Accession (Same CDS sequence)	NM_001348365.1 , XM_024447828.1
Accession Version	XM_024447828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAT CATCATCCGA AATGAAGGCC CCAGTCCTTC CAGAACCTAT TCTTCCTATC 
CAGCCCAAAA CTGTGAAAGA CTTTCAGGAA GATGTAGAAA AAGTTAAGTC ATCAGGAGAT 
TGGAAAGCAG TACATGATTT TTATCTAACA ACGTTTGATT CTTTCCCAGA ATTAAATGCT 
GCATTTAAGA AAGATGCCAC TGCCTCATTT AACACCATTG AAGACTCTGG GATTAATGCT 
AAATTTGTGA ATGCTGTGTA TGATACCTTA CTTAATACTC CTCAAGACGT TCAGAAGACA 
GTATTAAAGG GAATCATTAA CAGCCTGTTA CGAGAATGGA AAGGTCCACG AACAAAAGAT 
GATCTTAGAG CATATTTTAT ACTTTTACAG AATCCTCAGT TTAATAACAC ATCTACGTAT 
GTCATCTATG CTCACTTGCT ACGACAGATA GCTACCTTAG TGGAAGCTGA CCATCATTTC 
CTAGTTCACT GGTTTAAAAA ATTGTCCCAG AAGAGGTTCA AACAATTGGT AGAGAGATTG 
CTGCAATTTA TTTCTTTACG CCTGTTTCCT GCAAAGCCTG AAGAATTTCC ACCTATAACA 
AAGTGTTCCT GGTGGATTCC ATCAGCCGCT AAAGTGTTGG CTTTACTTAA TACTGCAAAC 
AATTTAGTTC ACCCTCCCCT TATTCCTTAT ACTGATTTCT ATAATTCTAC ATTGGATCAC 
ATTGATCTCA TGGAAGAATA TCATACTTGG CAAAACTTTG GAAACTCTCA CAGGTTTTCC 
TTCTGTCAGT ACCCATTCGT TATTTCTGTA GCTGCAAAAA AAATCATTAT TCAGAGAGAC 
TCAGAGCAAC AGATGATAAA CATCGCAAGG CAAAGTCTGG TGGATAAAGT ATCTCGAAGA 
CAGAGACCTG ATATGAATAT ATTATTTCTA AATATGAAAG TAAGACGGAC ACATCTGGTT 
AGCGATTCAC TTGATGAGCT AACCCGGAAA AGAGCCGATT TGAAAAAGAA GTTAAAAGTT 
ACATTTGTAG GGGAAGCTGG TTTGGATATG GGTGGTTTGA CTAAAGAATG GTTCCTTCTT 
CTAATTCGCC AAATTTTTCA TCCAGATTAT GGCATGTTTA CATATCACAA GGATTCACAC 
TGCCATTGGT TTAGCAGCTT TAAATGTGAT AACTATTCTG AATTCCGATT GGTTGGAATT 
CTTATGGGAC TAGCTGTTTA TAACAGCATC ACCTTGGATA TTCGTTTCCC TCCCTGCTGT 
TACAAGAAAT TATTGAGCCC TCCCATCATT CCTAGTGATC AAAATATACC AGTAGGCATC 
TGCAATGTTA CCGTGGACGA CTTATGTCAA ATTATGCCTG AGTTGGCCCA TGGATTAAGT 
GAACTCTTAT CACATGAAGG CAATGTTGAA GAAGATTTCT ATTCAACATT TCAGGTTTTT 
CAAGAAGAAT TTGGAATAAT CAAGTCCTAT AATTTAAAGC CCGGTGGTGA TAAAATTTCA 
GTTACCAATC AAAATAGAAA AGAATATGTA CAGCTTTATA CTGACTTCCT TCTGAACAAA 
TCCATCTATA AGCAGTTTGC TGCATTTTAT TATGGATTTC ATAGTGTGTG TGCTTCAAAT 
GCCCTAATGC TGCTTCGTCC AGAAGAGGTT GAAATTTTGG TCTGTGGAAG TCCTGACCTG 
GATATGCATG CTCTGCAGAG AAGTACTCAG TATGATGGCT ATGCAAAAAC GGACTTAACT 
ATAAAATACT TTTGGGATGT TGTGCTTGGA TTTCCTCTTG ATCTTCAAAA GAAGTTGCTA 
CATTTTACTA CAGGGAGTGA CAGAGTACCT GTAGGAGGGA TGGCTGATTT GAACTTTAAA 
ATCTCAAAGA ATGAAACTTC TACTAACTGC TTACCTGTGG CCCATACCTG CTTCAATCAA 
CTTTGCCTTC CCCCCTACAA GAGCAAAAAG GATCTGAAAC AGAAATTGAT CATTGGAATT 
TCAAATTCAG AAGGTTTTGG ACTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303596.1
CDS	313..2322
Translation	MDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLSQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLVHPPLIPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQMINIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGLSELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYAKTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_024447828.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447828.1

ORF Insert Sequence:

ATGGATGCAT CATCATCCGA AATGAAGGCC CCAGTCCTTC CAGAACCTAT TCTTCCTATC 
CAGCCCAAAA CTGTGAAAGA CTTTCAGGAA GATGTAGAAA AAGTTAAGTC ATCAGGAGAT 
TGGAAAGCAG TACATGATTT TTATCTAACA ACGTTTGATT CTTTCCCAGA ATTAAATGCT 
GCATTTAAGA AAGATGCCAC TGCCTCATTT AACACCATTG AAGACTCTGG GATTAATGCT 
AAATTTGTGA ATGCTGTGTA TGATACCTTA CTTAATACTC CTCAAGACGT TCAGAAGACA 
GTATTAAAGG GAATCATTAA CAGCCTGTTA CGAGAATGGA AAGGTCCACG AACAAAAGAT 
GATCTTAGAG CATATTTTAT ACTTTTACAG AATCCTCAGT TTAATAACAC ATCTACGTAT 
GTCATCTATG CTCACTTGCT ACGACAGATA GCTACCTTAG TGGAAGCTGA CCATCATTTC 
CTAGTTCACT GGTTTAAAAA ATTGTCCCAG AAGAGGTTCA AACAATTGGT AGAGAGATTG 
CTGCAATTTA TTTCTTTACG CCTGTTTCCT GCAAAGCCTG AAGAATTTCC ACCTATAACA 
AAGTGTTCCT GGTGGATTCC ATCAGCCGCT AAAGTGTTGG CTTTACTTAA TACTGCAAAC 
AATTTAGTTC ACCCTCCCCT TATTCCTTAT ACTGATTTCT ATAATTCTAC ATTGGATCAC 
ATTGATCTCA TGGAAGAATA TCATACTTGG CAAAACTTTG GAAACTCTCA CAGGTTTTCC 
TTCTGTCAGT ACCCATTCGT TATTTCTGTA GCTGCAAAAA AAATCATTAT TCAGAGAGAC 
TCAGAGCAAC AGATGATAAA CATCGCAAGG CAAAGTCTGG TGGATAAAGT ATCTCGAAGA 
CAGAGACCTG ATATGAATAT ATTATTTCTA AATATGAAAG TAAGACGGAC ACATCTGGTT 
AGCGATTCAC TTGATGAGCT AACCCGGAAA AGAGCCGATT TGAAAAAGAA GTTAAAAGTT 
ACATTTGTAG GGGAAGCTGG TTTGGATATG GGTGGTTTGA CTAAAGAATG GTTCCTTCTT 
CTAATTCGCC AAATTTTTCA TCCAGATTAT GGCATGTTTA CATATCACAA GGATTCACAC 
TGCCATTGGT TTAGCAGCTT TAAATGTGAT AACTATTCTG AATTCCGATT GGTTGGAATT 
CTTATGGGAC TAGCTGTTTA TAACAGCATC ACCTTGGATA TTCGTTTCCC TCCCTGCTGT 
TACAAGAAAT TATTGAGCCC TCCCATCATT CCTAGTGATC AAAATATACC AGTAGGCATC 
TGCAATGTTA CCGTGGACGA CTTATGTCAA ATTATGCCTG AGTTGGCCCA TGGATTAAGT 
GAACTCTTAT CACATGAAGG CAATGTTGAA GAAGATTTCT ATTCAACATT TCAGGTTTTT 
CAAGAAGAAT TTGGAATAAT CAAGTCCTAT AATTTAAAGC CCGGTGGTGA TAAAATTTCA 
GTTACCAATC AAAATAGAAA AGAATATGTA CAGCTTTATA CTGACTTCCT TCTGAACAAA 
TCCATCTATA AGCAGTTTGC TGCATTTTAT TATGGATTTC ATAGTGTGTG TGCTTCAAAT 
GCCCTAATGC TGCTTCGTCC AGAAGAGGTT GAAATTTTGG TCTGTGGAAG TCCTGACCTG 
GATATGCATG CTCTGCAGAG AAGTACTCAG TATGATGGCT ATGCAAAAAC GGACTTAACT 
ATAAAATACT TTTGGGATGT TGTGCTTGGA TTTCCTCTTG ATCTTCAAAA GAAGTTGCTA 
CATTTTACTA CAGGGAGTGA CAGAGTACCT GTAGGAGGGA TGGCTGATTT GAACTTTAAA 
ATCTCAAAGA ATGAAACTTC TACTAACTGC TTACCTGTGG CCCATACCTG CTTCAATCAA 
CTTTGCCTTC CCCCCTACAA GAGCAAAAAG GATCTGAAAC AGAAATTGAT CATTGGAATT 
TCAAATTCAG AAGGTTTTGG ACTTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-221 promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A.
Oncogene33(21)2790-800(2014 May)
Sun T,Wang X,He HH,Sweeney CJ,Liu SX,Brown M,Balk S,Lee GS,Kantoff PW

Absence of association between two HECTD2 polymorphisms and sporadic Creutzfeldt-Jakob disease.
Dementia and geriatric cognitive disorders31(2)146-51(2011)
Jeong BH,Lee KH,Lee YJ,Yun J,Park YJ,Cho HJ,Kim YH,Cho YS,Choi EK,Carp RI,Kim YS

HECTD2, a candidate susceptibility gene for Alzheimer's disease on 10q.
BMC medical genetics1090(2009 Sep)
Lloyd SE,Rossor M,Fox N,Mead S,Collinge J

Maturation of human dendritic cells is accompanied by functional remodelling of the ubiquitin-proteasome system.
The international journal of biochemistry & cell biology41(5)1205-15(2009 May)
Ebstein F,Lange N,Urban S,Seifert U,Kr?ger E,Kloetzel PM

HECTD2 is associated with susceptibility to mouse and human prion disease.
PLoS genetics5(2)e1000383(2009 Feb)
Lloyd SE,Maytham EG,Pota H,Grizenkova J,Molou E,Uphill J,Hummerich H,Whitfield J,Alpers MP,Mead S,Collinge J

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HECTD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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