TRPM6 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017014288.1	XP_016869777.1	transient receptor potential cation channel subfamily M member 6 isoform X9
XM_011518245.1	XP_011516547.1	transient receptor potential cation channel subfamily M member 6 isoform X2
XM_011518246.1	XP_011516548.1	transient receptor potential cation channel subfamily M member 6 isoform X3
XM_011518250.1	XP_011516552.1	transient receptor potential cation channel subfamily M member 6 isoform X7
NM_001177311.1	NP_001170782.1	transient receptor potential cation channel subfamily M member 6 isoform c
XM_011518247.1	XP_011516549.1	transient receptor potential cation channel subfamily M member 6 isoform X4
XM_011518249.1	XP_011516551.1	transient receptor potential cation channel subfamily M member 6 isoform X6
XM_011518252.2	XP_011516554.1	transient receptor potential cation channel subfamily M member 6 isoform X11
XM_011518251.2	XP_011516553.1	transient receptor potential cation channel subfamily M member 6 isoform X10
XM_011518244.1	XP_011516546.1	transient receptor potential cation channel subfamily M member 6 isoform X1
XM_017014287.1	XP_016869776.1	transient receptor potential cation channel subfamily M member 6 isoform X8
NM_017662.5	NP_060132.3	transient receptor potential cation channel subfamily M member 6 isoform a
NM_001177310.1	NP_001170781.1	transient receptor potential cation channel subfamily M member 6 isoform b
XM_011518248.1	XP_011516550.1	transient receptor potential cation channel subfamily M member 6 isoform X5
XM_017014289.1	XP_016869778.1	transient receptor potential cation channel subfamily M member 6 isoform X12
XM_011518255.2	XP_011516557.1	transient receptor potential cation channel subfamily M member 6 isoform X13
NM_017662.4	NP_060132.3	transient receptor potential cation channel subfamily M member 6 isoform a

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23065	NM_017662.5 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu75950	XM_011518255.2 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu75946	XM_011518251.2 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OHu98449	XM_017014287.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98450	XM_017014288.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98451	XM_017014289.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu75940	XM_011518245.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75941	XM_011518246.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75942	XM_011518247.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75943	XM_011518248.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75944	XM_011518249.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75945	XM_011518250.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu75947	XM_011518252.2 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu22680	NM_001177311.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu22981	NM_001177310.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu75939	XM_011518244.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu23065	NM_017662.4	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
Hide Old Accession Versions >>

TRPM6 ( NM_017662.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060132.3 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.

Price & Availability↑

CloneID	OHu23065
Clone ID Related Accession (Same CDS sequence)	NM_017662.5 , NM_017662.4
Accession Version	NM_017662.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF350881.2 and AL354795.17. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_017662.4. Transcript Variant: This variant (a) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF350881.2, AY333282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000360774.6/ ENSP00000354006.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC 
TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG 
CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT 
AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT 
TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG 
AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG 
ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA 
GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA 
GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG 
GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG 
GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA 
GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA 
AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA 
AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA 
GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG 
CAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060132.3
CDS	66..6134
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	NM_017662.5
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017662.5

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC 
TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG 
CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT 
AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT 
TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG 
AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG 
ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA 
GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA 
GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG 
GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG 
GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA 
GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA 
AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA 
AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA 
GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG 
CAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( NM_017662.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060132.3 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.

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CloneID	OHu23065
Clone ID Related Accession (Same CDS sequence)	NM_017662.5 , NM_017662.4
Accession Version	NM_017662.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF350881.2 and AL354795.17. This sequence is a reference standard in the RefSeqGene project. On Apr 17, 2008 this sequence version replaced NM_017662.3. Transcript Variant: This variant (a) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF350881.2, AY333282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC 
TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG 
CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT 
AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT 
TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG 
AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG 
ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA 
GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA 
GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG 
GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG 
GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA 
GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA 
AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA 
AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA 
GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG 
CAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060132.3
CDS	239..6307
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	NM_017662.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017662.4

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC 
TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG 
CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT 
AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT 
TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG 
AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG 
ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA 
GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA 
GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG 
GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG 
GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA 
GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA 
AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA 
AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA 
GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG 
CAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518255.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516557.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X15, mRNA.

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CloneID	OHu75950
Clone ID Related Accession (Same CDS sequence)	XM_011518255.2
Accession Version	XM_011518255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGATC 
TCCTACATGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516557.1
CDS	239..3793
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERISYMH

Target ORF information:

RefSeq Version	XM_011518255.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518255.2

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGATC 
TCCTACATGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518251.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516553.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu75946
Clone ID Related Accession (Same CDS sequence)	XM_011518251.2
Accession Version	XM_011518251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCGC ACTTCATCCT GTCTGATGAT GGGACCGTGG GCAAGTATGG AAATGAAATG 
AAGCTCAGAA GGAACCTGGA GAAGTACCTC TCTCTGCAGA AAATACACTG CCGCTCAAGA 
CAAGGCGTGC CGGTCGTGGG GCTGGTGGTG GAAGGCGGTC CCAACGTCAT CCTGTCAGTG 
TGGGAGACTG TCAAGGACAA GGACCCAGTG GTGGTGTGTG AGGGCACAGG TAGGGCGGCT 
GACCTCCTGG CCTTCACACA CAAACACCTG GCAGATGAAG GGATGCTGCG ACCTCAGGTG 
AAAGAGGAGA TCATCTGCAT GATTCAGAAC ACTTTCAACT TTAGTCTTAA ACAGTCCAAG 
CACCTTTTCC AAATTCTAAT GGAGTGTATG GTTCACAGGG ATTGTATTAC CATATTTGAT 
GCTGACTCTG AAGAGCAGCA AGACCTGGAC TTAGCAATCC TAACAGCTTT GCTGAAGGGC 
ACAAATTTAT CAGCGTCAGA GCAATTAAAT CTGGCAATGG CTTGGGACAG GGTGGACATT 
GCCAAGAAAC ATATCCTAAT TTATGAACAA CACTGGAAGC CTGATGCCCT GGAACAAGCA 
ATGTCAGATG CTTTAGTGAT GGATCGGGTG GATTTTGTGA AGCTCTTAAT AGAATATGGA 
GTGAACCTCC ATCGCTTTCT TACCATCCCT CGACTGGAAG AGCTCTACAA TACAAAACAA 
GGACCTACTA ATACACTCTT GCATCATCTC GTCCAAGATG TGAAACAGCA TACCCTTCTT 
TCAGGCTACC GAATAACCTT GATTGACATT GGATTAGTAG TAGAATACCT CATTGGTAGA 
GCATATCGCA GCAACTACAC TAGAAAACAT TTCAGAGCCC TCTACAACAA CCTCTACAGA 
AAATACAAGC ACCAGAGACA CTCCTCAGGA AATAGAAATG AGTCTGCAGA AAGTACGCTG 
CACTCCCAGT TCATTAGAAC TGCACAGCCA TACAAATTCA AGGAAAAGTC TATAGTCCTT 
CATAAATCAA GGAAGAAGTC AAAAGAACAA AATGTATCAG ATGACCCTGA GTCTACTGGC 
TTTCTTTACC CTTACAATGA CCTGCTGGTT TGGGCTGTGC TGATGAAAAG GCAGAAGATG 
GCTATGTTCT TCTGGCAGCA TGGAGAGGAG GCCACGGTTA AAGCCGTGAT TGCGTGTATC 
CTCTACCGGG CAATGGCCCA TGAAGCTAAG GAGAGTCACA TGGTGGATGA TGCCTCAGAA 
GAGTTGAAGA ATTACTCAAA ACAGTTTGGC CAGCTGGCTC TGGACTTGTT GGAGAAGGCA 
TTCAAGCAGA ATGAGCGCAT GGCCATGACG CTGTTGACGT ATGAACTCAG GAACTGGAGC 
AATTCGACCT GCCTTAAACT GGCCGTGTCG GGAGGATTAC GACCCTTTGT TTCACATACT 
TGTACCCAGA TGCTACTGAC AGACATGTGG ATGGGGAGGC TGAAAATGAG GAAAAACTCT 
TGGTTAAAGA TTATTATAAG CATTATTTTA CCACCCACCA TTTTGACACT GGAATTTAAA 
AGCAAAGCTG AGATGTCACA TGTTCCCCAG TCCCAGGACT TCCAATTTAT GTGGTATTAC 
AGTGACCAGA ACGCCAGCAG TTCCAAAGAA AGTGCTTCTG TGAAAGAGTA TGATTTGGAA 
AGGGGCCATG ATGAGAAACT GGATGAAAAT CAGCATTTTG GTTTGGAAAG TGGGCACCAA 
CACCTTCCGT GGACCAGGAA AGTCTATGAG TTCTACAGTG CTCCAATTGT CAAGTTTTGG 
TTTTATACGA TGGCGTATTT GGCATTCCTC ATGCTGTTCA CTTACACCGT GTTGGTGGAG 
ATGCAGCCCC AGCCCAGCGT GCAGGAGTGG CTTGTTAGCA TTTACATCTT CACCAATGCT 
ATTGAGGTGG TCAGGGAGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG 
GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT 
GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC 
ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA 
GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC 
ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG 
CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA 
GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT 
TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC 
CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG 
TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA 
CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT 
CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG 
AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA 
GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG 
TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT 
TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA 
GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT 
TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC 
AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG 
AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT 
ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA 
AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG 
TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT 
CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT 
CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC 
AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG 
CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC 
AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT 
GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT 
TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA 
ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA 
TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA 
TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA 
GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA 
AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT 
CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC 
AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG 
AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG 
TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG 
AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT 
CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG 
ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG 
GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA 
GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG 
AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC 
AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG 
CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC 
TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA 
ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC 
CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG 
ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT 
TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA 
TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT 
GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT 
GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT 
GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516553.1
CDS	219..5558
Translation	MHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518251.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518251.2

ORF Insert Sequence:

ATGCACTCGC ACTTCATCCT GTCTGATGAT GGGACCGTGG GCAAGTATGG AAATGAAATG 
AAGCTCAGAA GGAACCTGGA GAAGTACCTC TCTCTGCAGA AAATACACTG CCGCTCAAGA 
CAAGGCGTGC CGGTCGTGGG GCTGGTGGTG GAAGGCGGTC CCAACGTCAT CCTGTCAGTG 
TGGGAGACTG TCAAGGACAA GGACCCAGTG GTGGTGTGTG AGGGCACAGG TAGGGCGGCT 
GACCTCCTGG CCTTCACACA CAAACACCTG GCAGATGAAG GGATGCTGCG ACCTCAGGTG 
AAAGAGGAGA TCATCTGCAT GATTCAGAAC ACTTTCAACT TTAGTCTTAA ACAGTCCAAG 
CACCTTTTCC AAATTCTAAT GGAGTGTATG GTTCACAGGG ATTGTATTAC CATATTTGAT 
GCTGACTCTG AAGAGCAGCA AGACCTGGAC TTAGCAATCC TAACAGCTTT GCTGAAGGGC 
ACAAATTTAT CAGCGTCAGA GCAATTAAAT CTGGCAATGG CTTGGGACAG GGTGGACATT 
GCCAAGAAAC ATATCCTAAT TTATGAACAA CACTGGAAGC CTGATGCCCT GGAACAAGCA 
ATGTCAGATG CTTTAGTGAT GGATCGGGTG GATTTTGTGA AGCTCTTAAT AGAATATGGA 
GTGAACCTCC ATCGCTTTCT TACCATCCCT CGACTGGAAG AGCTCTACAA TACAAAACAA 
GGACCTACTA ATACACTCTT GCATCATCTC GTCCAAGATG TGAAACAGCA TACCCTTCTT 
TCAGGCTACC GAATAACCTT GATTGACATT GGATTAGTAG TAGAATACCT CATTGGTAGA 
GCATATCGCA GCAACTACAC TAGAAAACAT TTCAGAGCCC TCTACAACAA CCTCTACAGA 
AAATACAAGC ACCAGAGACA CTCCTCAGGA AATAGAAATG AGTCTGCAGA AAGTACGCTG 
CACTCCCAGT TCATTAGAAC TGCACAGCCA TACAAATTCA AGGAAAAGTC TATAGTCCTT 
CATAAATCAA GGAAGAAGTC AAAAGAACAA AATGTATCAG ATGACCCTGA GTCTACTGGC 
TTTCTTTACC CTTACAATGA CCTGCTGGTT TGGGCTGTGC TGATGAAAAG GCAGAAGATG 
GCTATGTTCT TCTGGCAGCA TGGAGAGGAG GCCACGGTTA AAGCCGTGAT TGCGTGTATC 
CTCTACCGGG CAATGGCCCA TGAAGCTAAG GAGAGTCACA TGGTGGATGA TGCCTCAGAA 
GAGTTGAAGA ATTACTCAAA ACAGTTTGGC CAGCTGGCTC TGGACTTGTT GGAGAAGGCA 
TTCAAGCAGA ATGAGCGCAT GGCCATGACG CTGTTGACGT ATGAACTCAG GAACTGGAGC 
AATTCGACCT GCCTTAAACT GGCCGTGTCG GGAGGATTAC GACCCTTTGT TTCACATACT 
TGTACCCAGA TGCTACTGAC AGACATGTGG ATGGGGAGGC TGAAAATGAG GAAAAACTCT 
TGGTTAAAGA TTATTATAAG CATTATTTTA CCACCCACCA TTTTGACACT GGAATTTAAA 
AGCAAAGCTG AGATGTCACA TGTTCCCCAG TCCCAGGACT TCCAATTTAT GTGGTATTAC 
AGTGACCAGA ACGCCAGCAG TTCCAAAGAA AGTGCTTCTG TGAAAGAGTA TGATTTGGAA 
AGGGGCCATG ATGAGAAACT GGATGAAAAT CAGCATTTTG GTTTGGAAAG TGGGCACCAA 
CACCTTCCGT GGACCAGGAA AGTCTATGAG TTCTACAGTG CTCCAATTGT CAAGTTTTGG 
TTTTATACGA TGGCGTATTT GGCATTCCTC ATGCTGTTCA CTTACACCGT GTTGGTGGAG 
ATGCAGCCCC AGCCCAGCGT GCAGGAGTGG CTTGTTAGCA TTTACATCTT CACCAATGCT 
ATTGAGGTGG TCAGGGAGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG 
GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT 
GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC 
ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA 
GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC 
ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG 
CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA 
GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT 
TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC 
CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG 
TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA 
CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT 
CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG 
AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA 
GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG 
TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT 
TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA 
GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT 
TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC 
AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG 
AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT 
ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA 
AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG 
TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT 
CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT 
CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC 
AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG 
CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC 
AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT 
GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT 
TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA 
ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA 
TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA 
TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA 
GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA 
AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT 
CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC 
AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG 
AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG 
TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG 
AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT 
CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG 
ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG 
GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA 
GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG 
AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC 
AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG 
CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC 
TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA 
ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC 
CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG 
ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT 
TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA 
TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT 
GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT 
GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT 
GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_017014287.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869776.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X9, mRNA.

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CloneID	OHu98449
Clone ID Related Accession (Same CDS sequence)	XM_017014287.1
Accession Version	XM_017014287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA 
AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC 
CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG 
AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC 
GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG 
GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC 
TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA 
CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC 
TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA 
ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG 
ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA 
TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA 
GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG 
GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA 
ATTGTCAAGT TTTGGTTTTA TACGATCTGT ATTTCAGAAC CTGGGAAGTT TACCCAAAAG 
GTGAAGGTAT GGATTAGTGA GTACTGGAAC TTAACAGAAA CTGTGGCCAT TGGCCTGTTT 
TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC CCTCCTTTTC ACACAGCGGG AAGACTGATC 
TACTGCATAG ACATCATATT CTGGTTCTCA CGGCTCCTGG ACTTCTTTGC TGTGAATCAA 
CATGCAGGTC CATATGTGAC CATGATTGCA AAAATGACAG CAAACATGTT CTATATTGTG 
ATCATCATGG CCATAGTCCT GCTGAGCTTT GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA 
AAAGAGCCAC CATCTTGGAG TCTAGCTCGA GATATTGTAT TTGAGCCATA CTGGATGATA 
TACGGAGAAG TCTATGCTGG AGAAATAGAT GTTTGTTCAA GCCAGCCATC CTGCCCTCCT 
GGTTCTTTTC TTACTCCATT CTTGCAAGCT GTCTACCTCT TCGTGCAATA TATCATCATG 
GTGAACCTGT TGATTGCTTT CTTCAACAAC GTTTACTTAG ATATGGAATC CATTTCAAAT 
AACCTGTGGA AATACAACCG CTATCGCTAC ATCATGACCT ACCACGAGAA GCCCTGGCTG 
CCCCCACCTC TCATCCTGCT GAGCCACGTG GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT 
CGAGCTCCTC ACGACCAAGA AGAGGGTGAC GTTGGATTAA AACTCTACCT CAGTAAGGAG 
GATCTGAAAA AACTTCATGA TTTTGAGGAG CAGTGCGTGG AAAAATACTT CCATGAGAAG 
ATGGAAGATG TGAATTGTAG TTGTGAGGAA CGAATCCGAG TGACATCAGA AAGGGTTACA 
GAGATGTACT TCCAGCTGAA AGAAATGAAT GAAAAGGTGT CTTTTATAAA GGACTCCTTA 
CTGTCTTTGG ACAGCCAGGT GGGACACCTG CAGGATCTCT CTGCCCTGAC TGTGGATACC 
CTGAAAGTCC TTTCTGCTGT TGACACTTTG CAAGAGGATG AGGCTCTCCT GGCCAAGAGA 
AAGCATTCTA CTTGCAAAAA ACTTCCCCAC AGCTGGAGCA ATGTCATCTG TGCAGAGGTT 
CTAGGCAGCA TGGAGATCGC TGGAGAGAAG AAATACCAGT ATTATAGCAT GCCCTCTTCT 
TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT CCCCCAAGAG TGCAGAGGGG GGCACTTCTT 
GAGATTACAA ACAGTAAAAG AGAGGCTACA AATGTAAGAA ATGACCAGGA AAGGCAAGAA 
ACACAAAGTA GTATAGTGGT TTCTGGGGTG TCTCCTAACA GGCAAGCACA CTCAAAGTAT 
GGCCAGTTTC TTCTGGTCCC CTCTAATCTA AAGCGAGTTC CTTTTTCAGC AGAAACTGTC 
TTGCCTCTGT CCAGACCCTC TGTGCCAGAT GTGCTGGCAA CTGAACAGGA CATCCAGACT 
GAGGTTCTTG TTCATCTGAC TGGGCAGACC CCAGTTGTCT CTGACTGGGC ATCAGTGGAT 
GAACCCAAGG AAAAGCACGA GCCTATTGCT CACTTACTGG ATGGACAAGA CAAGGCAGAG 
CAAGTGCTAC CCACTTTGAG TTGCACACCT GAACCCATGA CAATGAGCTC CCCTCTTTCC 
CAAGCCAAGA TCATGCAAAC TGGAGGTGGA TATGTAAACT GGGCATTTTC AGAAGGTGAT 
GAAACTGGTG TGTTTAGCAT CAAGAAAAAG TGGCAAACCT GCTTGCCCTC CACTTGTGAC 
AGTGATTCCT CTCGGAGTGA ACAGCACCAG AAGCAGGCCC AGGACAGCTC CCTATCTGAT 
AACTCAACAA GATCGGCCCA GAGTAGTGAA TGCTCAGAGG TGGGACCATG GCTTCAGCCA 
AACACATCCT TTTGGATCAA TCCTCTCCGC AGATACAGGC CCTTCGCTAG GAGTCATAGT 
TTTAGATTCC ATAAGGAGGA GAAATTGATG AAGATCTGTA AGATTAAAAA TCTTTCAGGC 
TCTTCAGAAA TAGGGCAGGG AGCATGGGTC AAAGCGAAAA TGCTAACCAA AGACAGGAGA 
CTGTCAAAGA AAAAGAAGAA TACTCAAGGA CTCCAGGTGC CAATCATAAC AGTCAATGCC 
TGCTCTCAGA GTGACCAGTT GAATCCAGAG CCAGGAGAAA ACAGCATCTC TGAAGAGGAG 
TACAGCAAGA ACTGGTTCAC AGTGTCCAAA TTTAGTCACA CAGGTGTAGA ACCTTACATA 
CATCAGAAAA TGAAAACTAA AGAAATTGGA CAATGTGCTA TACAAATCAG TGATTACCTA 
AAGCAGTCTC AAGAGGATCT CAGCAAAAAC TCTTTGTGGA ATTCCAGGAG CACCAACCTC 
AATAGGAACT CCCTGCTGAA AAGTTCAATT GGAGTTGACA AGATCTCAGC CTCCTTAAAA 
AGCCCTCAAG AGCCTCACCA TCATTATTCA GCCATTGAAA GGAATAATTT AATGAGGCTT 
TCTCAGACCA TACCATTTAC ACCAGTCCAA CTGTTTGCAG GAGAAGAAAT AACTGTCTAC 
AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT 
GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA 
GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC 
ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC 
ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC 
TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT 
TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG 
GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG 
GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT 
TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC 
AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC 
TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA 
AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA 
TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869776.1
CDS	239..5944
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_017014287.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014287.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA 
AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC 
CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG 
AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC 
GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG 
GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC 
TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA 
CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC 
TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA 
ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG 
ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA 
TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA 
GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG 
GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA 
ATTGTCAAGT TTTGGTTTTA TACGATCTGT ATTTCAGAAC CTGGGAAGTT TACCCAAAAG 
GTGAAGGTAT GGATTAGTGA GTACTGGAAC TTAACAGAAA CTGTGGCCAT TGGCCTGTTT 
TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC CCTCCTTTTC ACACAGCGGG AAGACTGATC 
TACTGCATAG ACATCATATT CTGGTTCTCA CGGCTCCTGG ACTTCTTTGC TGTGAATCAA 
CATGCAGGTC CATATGTGAC CATGATTGCA AAAATGACAG CAAACATGTT CTATATTGTG 
ATCATCATGG CCATAGTCCT GCTGAGCTTT GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA 
AAAGAGCCAC CATCTTGGAG TCTAGCTCGA GATATTGTAT TTGAGCCATA CTGGATGATA 
TACGGAGAAG TCTATGCTGG AGAAATAGAT GTTTGTTCAA GCCAGCCATC CTGCCCTCCT 
GGTTCTTTTC TTACTCCATT CTTGCAAGCT GTCTACCTCT TCGTGCAATA TATCATCATG 
GTGAACCTGT TGATTGCTTT CTTCAACAAC GTTTACTTAG ATATGGAATC CATTTCAAAT 
AACCTGTGGA AATACAACCG CTATCGCTAC ATCATGACCT ACCACGAGAA GCCCTGGCTG 
CCCCCACCTC TCATCCTGCT GAGCCACGTG GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT 
CGAGCTCCTC ACGACCAAGA AGAGGGTGAC GTTGGATTAA AACTCTACCT CAGTAAGGAG 
GATCTGAAAA AACTTCATGA TTTTGAGGAG CAGTGCGTGG AAAAATACTT CCATGAGAAG 
ATGGAAGATG TGAATTGTAG TTGTGAGGAA CGAATCCGAG TGACATCAGA AAGGGTTACA 
GAGATGTACT TCCAGCTGAA AGAAATGAAT GAAAAGGTGT CTTTTATAAA GGACTCCTTA 
CTGTCTTTGG ACAGCCAGGT GGGACACCTG CAGGATCTCT CTGCCCTGAC TGTGGATACC 
CTGAAAGTCC TTTCTGCTGT TGACACTTTG CAAGAGGATG AGGCTCTCCT GGCCAAGAGA 
AAGCATTCTA CTTGCAAAAA ACTTCCCCAC AGCTGGAGCA ATGTCATCTG TGCAGAGGTT 
CTAGGCAGCA TGGAGATCGC TGGAGAGAAG AAATACCAGT ATTATAGCAT GCCCTCTTCT 
TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT CCCCCAAGAG TGCAGAGGGG GGCACTTCTT 
GAGATTACAA ACAGTAAAAG AGAGGCTACA AATGTAAGAA ATGACCAGGA AAGGCAAGAA 
ACACAAAGTA GTATAGTGGT TTCTGGGGTG TCTCCTAACA GGCAAGCACA CTCAAAGTAT 
GGCCAGTTTC TTCTGGTCCC CTCTAATCTA AAGCGAGTTC CTTTTTCAGC AGAAACTGTC 
TTGCCTCTGT CCAGACCCTC TGTGCCAGAT GTGCTGGCAA CTGAACAGGA CATCCAGACT 
GAGGTTCTTG TTCATCTGAC TGGGCAGACC CCAGTTGTCT CTGACTGGGC ATCAGTGGAT 
GAACCCAAGG AAAAGCACGA GCCTATTGCT CACTTACTGG ATGGACAAGA CAAGGCAGAG 
CAAGTGCTAC CCACTTTGAG TTGCACACCT GAACCCATGA CAATGAGCTC CCCTCTTTCC 
CAAGCCAAGA TCATGCAAAC TGGAGGTGGA TATGTAAACT GGGCATTTTC AGAAGGTGAT 
GAAACTGGTG TGTTTAGCAT CAAGAAAAAG TGGCAAACCT GCTTGCCCTC CACTTGTGAC 
AGTGATTCCT CTCGGAGTGA ACAGCACCAG AAGCAGGCCC AGGACAGCTC CCTATCTGAT 
AACTCAACAA GATCGGCCCA GAGTAGTGAA TGCTCAGAGG TGGGACCATG GCTTCAGCCA 
AACACATCCT TTTGGATCAA TCCTCTCCGC AGATACAGGC CCTTCGCTAG GAGTCATAGT 
TTTAGATTCC ATAAGGAGGA GAAATTGATG AAGATCTGTA AGATTAAAAA TCTTTCAGGC 
TCTTCAGAAA TAGGGCAGGG AGCATGGGTC AAAGCGAAAA TGCTAACCAA AGACAGGAGA 
CTGTCAAAGA AAAAGAAGAA TACTCAAGGA CTCCAGGTGC CAATCATAAC AGTCAATGCC 
TGCTCTCAGA GTGACCAGTT GAATCCAGAG CCAGGAGAAA ACAGCATCTC TGAAGAGGAG 
TACAGCAAGA ACTGGTTCAC AGTGTCCAAA TTTAGTCACA CAGGTGTAGA ACCTTACATA 
CATCAGAAAA TGAAAACTAA AGAAATTGGA CAATGTGCTA TACAAATCAG TGATTACCTA 
AAGCAGTCTC AAGAGGATCT CAGCAAAAAC TCTTTGTGGA ATTCCAGGAG CACCAACCTC 
AATAGGAACT CCCTGCTGAA AAGTTCAATT GGAGTTGACA AGATCTCAGC CTCCTTAAAA 
AGCCCTCAAG AGCCTCACCA TCATTATTCA GCCATTGAAA GGAATAATTT AATGAGGCTT 
TCTCAGACCA TACCATTTAC ACCAGTCCAA CTGTTTGCAG GAGAAGAAAT AACTGTCTAC 
AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT 
GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA 
GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC 
ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC 
ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC 
TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT 
TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG 
GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG 
GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT 
TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC 
AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC 
TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA 
AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA 
TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_017014288.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869777.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X10, mRNA.

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CloneID	OHu98450
Clone ID Related Accession (Same CDS sequence)	XM_017014288.1
Accession Version	XM_017014288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA 
AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC 
CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG 
AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC 
GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG 
GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC 
TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA 
CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC 
TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA 
ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG 
ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA 
TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGATC 
TGTATTTCAG AACCTGGGAA GTTTACCCAA AAGGTGAAGG TATGGATTAG TGAGTACTGG 
AACTTAACAG AAACTGTGGC CATTGGCCTG TTTTCAGCTG GCTTCGTCCT TCGATGGGGT 
GACCCTCCTT TTCACACAGC GGGAAGACTG ATCTACTGCA TAGACATCAT ATTCTGGTTC 
TCACGGCTCC TGGACTTCTT TGCTGTGAAT CAACATGCAG GTCCATATGT GACCATGATT 
GCAAAAATGA CAGCAAACAT GTTCTATATT GTGATCATCA TGGCCATAGT CCTGCTGAGC 
TTTGGAGTGG CACGCAAGGC CATCCTTTCG CCAAAAGAGC CACCATCTTG GAGTCTAGCT 
CGAGATATTG TATTTGAGCC ATACTGGATG ATATACGGAG AAGTCTATGC TGGAGAAATA 
GATGTTTGTT CAAGCCAGCC ATCCTGCCCT CCTGGTTCTT TTCTTACTCC ATTCTTGCAA 
GCTGTCTACC TCTTCGTGCA ATATATCATC ATGGTGAACC TGTTGATTGC TTTCTTCAAC 
AACGTTTACT TAGATATGGA ATCCATTTCA AATAACCTGT GGAAATACAA CCGCTATCGC 
TACATCATGA CCTACCACGA GAAGCCCTGG CTGCCCCCAC CTCTCATCCT GCTGAGCCAC 
GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT CATCGAGCTC CTCACGACCA AGAAGAGGGT 
GACGTTGGAT TAAAACTCTA CCTCAGTAAG GAGGATCTGA AAAAACTTCA TGATTTTGAG 
GAGCAGTGCG TGGAAAAATA CTTCCATGAG AAGATGGAAG ATGTGAATTG TAGTTGTGAG 
GAACGAATCC GAGTGACATC AGAAAGGGTT ACAGAGATGT ACTTCCAGCT GAAAGAAATG 
AATGAAAAGG TGTCTTTTAT AAAGGACTCC TTACTGTCTT TGGACAGCCA GGTGGGACAC 
CTGCAGGATC TCTCTGCCCT GACTGTGGAT ACCCTGAAAG TCCTTTCTGC TGTTGACACT 
TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG AGAAAGCATT CTACTTGCAA AAAACTTCCC 
CACAGCTGGA GCAATGTCAT CTGTGCAGAG GTTCTAGGCA GCATGGAGAT CGCTGGAGAG 
AAGAAATACC AGTATTATAG CATGCCCTCT TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG 
CATCCCCCAA GAGTGCAGAG GGGGGCACTT CTTGAGATTA CAAACAGTAA AAGAGAGGCT 
ACAAATGTAA GAAATGACCA GGAAAGGCAA GAAACACAAA GTAGTATAGT GGTTTCTGGG 
GTGTCTCCTA ACAGGCAAGC ACACTCAAAG TATGGCCAGT TTCTTCTGGT CCCCTCTAAT 
CTAAAGCGAG TTCCTTTTTC AGCAGAAACT GTCTTGCCTC TGTCCAGACC CTCTGTGCCA 
GATGTGCTGG CAACTGAACA GGACATCCAG ACTGAGGTTC TTGTTCATCT GACTGGGCAG 
ACCCCAGTTG TCTCTGACTG GGCATCAGTG GATGAACCCA AGGAAAAGCA CGAGCCTATT 
GCTCACTTAC TGGATGGACA AGACAAGGCA GAGCAAGTGC TACCCACTTT GAGTTGCACA 
CCTGAACCCA TGACAATGAG CTCCCCTCTT TCCCAAGCCA AGATCATGCA AACTGGAGGT 
GGATATGTAA ACTGGGCATT TTCAGAAGGT GATGAAACTG GTGTGTTTAG CATCAAGAAA 
AAGTGGCAAA CCTGCTTGCC CTCCACTTGT GACAGTGATT CCTCTCGGAG TGAACAGCAC 
CAGAAGCAGG CCCAGGACAG CTCCCTATCT GATAACTCAA CAAGATCGGC CCAGAGTAGT 
GAATGCTCAG AGGTGGGACC ATGGCTTCAG CCAAACACAT CCTTTTGGAT CAATCCTCTC 
CGCAGATACA GGCCCTTCGC TAGGAGTCAT AGTTTTAGAT TCCATAAGGA GGAGAAATTG 
ATGAAGATCT GTAAGATTAA AAATCTTTCA GGCTCTTCAG AAATAGGGCA GGGAGCATGG 
GTCAAAGCGA AAATGCTAAC CAAAGACAGG AGACTGTCAA AGAAAAAGAA GAATACTCAA 
GGACTCCAGG TGCCAATCAT AACAGTCAAT GCCTGCTCTC AGAGTGACCA GTTGAATCCA 
GAGCCAGGAG AAAACAGCAT CTCTGAAGAG GAGTACAGCA AGAACTGGTT CACAGTGTCC 
AAATTTAGTC ACACAGGTGT AGAACCTTAC ATACATCAGA AAATGAAAAC TAAAGAAATT 
GGACAATGTG CTATACAAAT CAGTGATTAC CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA 
AACTCTTTGT GGAATTCCAG GAGCACCAAC CTCAATAGGA ACTCCCTGCT GAAAAGTTCA 
ATTGGAGTTG ACAAGATCTC AGCCTCCTTA AAAAGCCCTC AAGAGCCTCA CCATCATTAT 
TCAGCCATTG AAAGGAATAA TTTAATGAGG CTTTCTCAGA CCATACCATT TACACCAGTC 
CAACTGTTTG CAGGAGAAGA AATAACTGTC TACAGGTTGG AGGAGAGTTC CCCTTTAAAC 
CTTGATAAAA GCATGTCCTC TTGGTCTCAG CGTGGGAGAG CGGCAATGAT CCAGGTATTG 
TCCCGAGAGG AGATGGATGG GGGCCTCCGT AAAGCTATGA GAGTCGTCAG CACTTGGTCT 
GAGGATGACA TTCTCAAGCC GGGACAAGTT TTCATTGTCA AGTCCTTTCT TCCTGAGGTT 
GTGCGGACAT GGCATAAAAT CTTCCAGGAG AGCACTGTGC TTCATCTTTG CCTCAGGGAA 
ATTCAACAAC AAAGAGCTGC TCAAAAATTG ATCTATACCT TCAACCAAGT GAAACCACAA 
ACCATACCCT ACACACCAAG GTTCCTGGAA GTTTTCTTAA TCTACTGCCA TTCAGCCAAC 
CAGTGGTTGA CCATTGAGAA GTATATGACA GGGGAGTTCC GGAAGTATAA CAACAACAAT 
GGTGATGAAA TCACCCCCAC CAACACCCTG GAGGAGCTGA TGTTGGCTTT CTCTCACTGG 
ACCTATGAGT ACACTCGGGG AGAGCTGCTG GTTTTAGATT TGCAAGGTGT TGGAGAAAAT 
TTGACAGATC CATCTGTTAT AAAACCTGAA GTCAAACAAT CAAGAGGAAT GGTGTTTGGA 
CCGGCCAATT TGGGGGAAGA TGCAATTAGA AACTTCATTG CAAAACATCA TTGTAACTCC 
TGCTGCCGGA AGCTCAAACT CCCGGATTTA AAAAGAAATG ACTATTCCCC TGAAAGGATA 
AATTCCACCT TTGGACTTGA GATAAAAATA GAATCAGCTG AGGAGCCTCC AGCAAGGGAG 
ACGGGTAGAA ATTCCCCAGA AGATGATATG CAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869777.1
CDS	239..5797
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_017014288.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014288.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA 
AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC 
CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG 
AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC 
GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG 
GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC 
TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA 
CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC 
TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA 
ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG 
ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA 
TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGATC 
TGTATTTCAG AACCTGGGAA GTTTACCCAA AAGGTGAAGG TATGGATTAG TGAGTACTGG 
AACTTAACAG AAACTGTGGC CATTGGCCTG TTTTCAGCTG GCTTCGTCCT TCGATGGGGT 
GACCCTCCTT TTCACACAGC GGGAAGACTG ATCTACTGCA TAGACATCAT ATTCTGGTTC 
TCACGGCTCC TGGACTTCTT TGCTGTGAAT CAACATGCAG GTCCATATGT GACCATGATT 
GCAAAAATGA CAGCAAACAT GTTCTATATT GTGATCATCA TGGCCATAGT CCTGCTGAGC 
TTTGGAGTGG CACGCAAGGC CATCCTTTCG CCAAAAGAGC CACCATCTTG GAGTCTAGCT 
CGAGATATTG TATTTGAGCC ATACTGGATG ATATACGGAG AAGTCTATGC TGGAGAAATA 
GATGTTTGTT CAAGCCAGCC ATCCTGCCCT CCTGGTTCTT TTCTTACTCC ATTCTTGCAA 
GCTGTCTACC TCTTCGTGCA ATATATCATC ATGGTGAACC TGTTGATTGC TTTCTTCAAC 
AACGTTTACT TAGATATGGA ATCCATTTCA AATAACCTGT GGAAATACAA CCGCTATCGC 
TACATCATGA CCTACCACGA GAAGCCCTGG CTGCCCCCAC CTCTCATCCT GCTGAGCCAC 
GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT CATCGAGCTC CTCACGACCA AGAAGAGGGT 
GACGTTGGAT TAAAACTCTA CCTCAGTAAG GAGGATCTGA AAAAACTTCA TGATTTTGAG 
GAGCAGTGCG TGGAAAAATA CTTCCATGAG AAGATGGAAG ATGTGAATTG TAGTTGTGAG 
GAACGAATCC GAGTGACATC AGAAAGGGTT ACAGAGATGT ACTTCCAGCT GAAAGAAATG 
AATGAAAAGG TGTCTTTTAT AAAGGACTCC TTACTGTCTT TGGACAGCCA GGTGGGACAC 
CTGCAGGATC TCTCTGCCCT GACTGTGGAT ACCCTGAAAG TCCTTTCTGC TGTTGACACT 
TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG AGAAAGCATT CTACTTGCAA AAAACTTCCC 
CACAGCTGGA GCAATGTCAT CTGTGCAGAG GTTCTAGGCA GCATGGAGAT CGCTGGAGAG 
AAGAAATACC AGTATTATAG CATGCCCTCT TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG 
CATCCCCCAA GAGTGCAGAG GGGGGCACTT CTTGAGATTA CAAACAGTAA AAGAGAGGCT 
ACAAATGTAA GAAATGACCA GGAAAGGCAA GAAACACAAA GTAGTATAGT GGTTTCTGGG 
GTGTCTCCTA ACAGGCAAGC ACACTCAAAG TATGGCCAGT TTCTTCTGGT CCCCTCTAAT 
CTAAAGCGAG TTCCTTTTTC AGCAGAAACT GTCTTGCCTC TGTCCAGACC CTCTGTGCCA 
GATGTGCTGG CAACTGAACA GGACATCCAG ACTGAGGTTC TTGTTCATCT GACTGGGCAG 
ACCCCAGTTG TCTCTGACTG GGCATCAGTG GATGAACCCA AGGAAAAGCA CGAGCCTATT 
GCTCACTTAC TGGATGGACA AGACAAGGCA GAGCAAGTGC TACCCACTTT GAGTTGCACA 
CCTGAACCCA TGACAATGAG CTCCCCTCTT TCCCAAGCCA AGATCATGCA AACTGGAGGT 
GGATATGTAA ACTGGGCATT TTCAGAAGGT GATGAAACTG GTGTGTTTAG CATCAAGAAA 
AAGTGGCAAA CCTGCTTGCC CTCCACTTGT GACAGTGATT CCTCTCGGAG TGAACAGCAC 
CAGAAGCAGG CCCAGGACAG CTCCCTATCT GATAACTCAA CAAGATCGGC CCAGAGTAGT 
GAATGCTCAG AGGTGGGACC ATGGCTTCAG CCAAACACAT CCTTTTGGAT CAATCCTCTC 
CGCAGATACA GGCCCTTCGC TAGGAGTCAT AGTTTTAGAT TCCATAAGGA GGAGAAATTG 
ATGAAGATCT GTAAGATTAA AAATCTTTCA GGCTCTTCAG AAATAGGGCA GGGAGCATGG 
GTCAAAGCGA AAATGCTAAC CAAAGACAGG AGACTGTCAA AGAAAAAGAA GAATACTCAA 
GGACTCCAGG TGCCAATCAT AACAGTCAAT GCCTGCTCTC AGAGTGACCA GTTGAATCCA 
GAGCCAGGAG AAAACAGCAT CTCTGAAGAG GAGTACAGCA AGAACTGGTT CACAGTGTCC 
AAATTTAGTC ACACAGGTGT AGAACCTTAC ATACATCAGA AAATGAAAAC TAAAGAAATT 
GGACAATGTG CTATACAAAT CAGTGATTAC CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA 
AACTCTTTGT GGAATTCCAG GAGCACCAAC CTCAATAGGA ACTCCCTGCT GAAAAGTTCA 
ATTGGAGTTG ACAAGATCTC AGCCTCCTTA AAAAGCCCTC AAGAGCCTCA CCATCATTAT 
TCAGCCATTG AAAGGAATAA TTTAATGAGG CTTTCTCAGA CCATACCATT TACACCAGTC 
CAACTGTTTG CAGGAGAAGA AATAACTGTC TACAGGTTGG AGGAGAGTTC CCCTTTAAAC 
CTTGATAAAA GCATGTCCTC TTGGTCTCAG CGTGGGAGAG CGGCAATGAT CCAGGTATTG 
TCCCGAGAGG AGATGGATGG GGGCCTCCGT AAAGCTATGA GAGTCGTCAG CACTTGGTCT 
GAGGATGACA TTCTCAAGCC GGGACAAGTT TTCATTGTCA AGTCCTTTCT TCCTGAGGTT 
GTGCGGACAT GGCATAAAAT CTTCCAGGAG AGCACTGTGC TTCATCTTTG CCTCAGGGAA 
ATTCAACAAC AAAGAGCTGC TCAAAAATTG ATCTATACCT TCAACCAAGT GAAACCACAA 
ACCATACCCT ACACACCAAG GTTCCTGGAA GTTTTCTTAA TCTACTGCCA TTCAGCCAAC 
CAGTGGTTGA CCATTGAGAA GTATATGACA GGGGAGTTCC GGAAGTATAA CAACAACAAT 
GGTGATGAAA TCACCCCCAC CAACACCCTG GAGGAGCTGA TGTTGGCTTT CTCTCACTGG 
ACCTATGAGT ACACTCGGGG AGAGCTGCTG GTTTTAGATT TGCAAGGTGT TGGAGAAAAT 
TTGACAGATC CATCTGTTAT AAAACCTGAA GTCAAACAAT CAAGAGGAAT GGTGTTTGGA 
CCGGCCAATT TGGGGGAAGA TGCAATTAGA AACTTCATTG CAAAACATCA TTGTAACTCC 
TGCTGCCGGA AGCTCAAACT CCCGGATTTA AAAAGAAATG ACTATTCCCC TGAAAGGATA 
AATTCCACCT TTGGACTTGA GATAAAAATA GAATCAGCTG AGGAGCCTCC AGCAAGGGAG 
ACGGGTAGAA ATTCCCCAGA AGATGATATG CAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_017014289.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869778.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X14, mRNA.

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CloneID	OHu98451
Clone ID Related Accession (Same CDS sequence)	XM_017014289.1
Accession Version	XM_017014289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAGTAAGGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869778.1
CDS	239..4921
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKSKE

Target ORF information:

RefSeq Version	XM_017014289.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014289.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAGTAAGGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518245.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516547.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA.

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CloneID	OHu75940
Clone ID Related Accession (Same CDS sequence)	XM_011518245.1
Accession Version	XM_011518245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGGA AAAGTCTATA GTCCTTCATA AATCAAGGAA GAAGTCAAAA 
GAACAAAATG TATCAGATGA CCCTGAGTCT ACTGGCTTTC TTTACCCTTA CAATGACCTG 
CTGGTTTGGG CTGTGCTGAT GAAAAGGCAG AAGATGGCTA TGTTCTTCTG GCAGCATGGA 
GAGGAGGCCA CGGTTAAAGC CGTGATTGCG TGTATCCTCT ACCGGGCAAT GGCCCATGAA 
GCTAAGGAGA GTCACATGGT GGATGATGCC TCAGAAGAGT TGAAGAATTA CTCAAAACAG 
TTTGGCCAGC TGGCTCTGGA CTTGTTGGAG AAGGCATTCA AGCAGAATGA GCGCATGGCC 
ATGACGCTGT TGACGTATGA ACTCAGGAAC TGGAGCAATT CGACCTGCCT TAAACTGGCC 
GTGTCGGGAG GATTACGACC CTTTGTTTCA CATACTTGTA CCCAGATGCT ACTGACAGAC 
ATGTGGATGG GGAGGCTGAA AATGAGGAAA AACTCTTGGT TAAAGATTAT TATAAGCATT 
ATTTTACCAC CCACCATTTT GACACTGGAA TTTAAAAGCA AAGCTGAGAT GTCACATGTT 
CCCCAGTCCC AGGACTTCCA ATTTATGTGG TATTACAGTG ACCAGAACGC CAGCAGTTCC 
AAAGAAAGTG CTTCTGTGAA AGAGTATGAT TTGGAAAGGG GCCATGATGA GAAACTGGAT 
GAAAATCAGC ATTTTGGTTT GGAAAGTGGG CACCAACACC TTCCGTGGAC CAGGAAAGTC 
TATGAGTTCT ACAGTGCTCC AATTGTCAAG TTTTGGTTTT ATACGATGGC GTATTTGGCA 
TTCCTCATGC TGTTCACTTA CACCGTGTTG GTGGAGATGC AGCCCCAGCC CAGCGTGCAG 
GAGTGGCTTG TTAGCATTTA CATCTTCACC AATGCTATTG AGGTGGTCAG GGAGATCTGT 
ATTTCAGAAC CTGGGAAGTT TACCCAAAAG GTGAAGGTAT GGATTAGTGA GTACTGGAAC 
TTAACAGAAA CTGTGGCCAT TGGCCTGTTT TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC 
CCTCCTTTTC ACACAGCGGG AAGACTGATC TACTGCATAG ACATCATATT CTGGTTCTCA 
CGGCTCCTGG ACTTCTTTGC TGTGAATCAA CATGCAGGTC CATATGTGAC CATGATTGCA 
AAAATGACAG CAAACATGTT CTATATTGTG ATCATCATGG CCATAGTCCT GCTGAGCTTT 
GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA AAAGAGCCAC CATCTTGGAG TCTAGCTCGA 
GATATTGTAT TTGAGCCATA CTGGATGATA TACGGAGAAG TCTATGCTGG AGAAATAGAT 
GTTTGTTCAA GCCAGCCATC CTGCCCTCCT GGTTCTTTTC TTACTCCATT CTTGCAAGCT 
GTCTACCTCT TCGTGCAATA TATCATCATG GTGAACCTGT TGATTGCTTT CTTCAACAAC 
GTTTACTTAG ATATGGAATC CATTTCAAAT AACCTGTGGA AATACAACCG CTATCGCTAC 
ATCATGACCT ACCACGAGAA GCCCTGGCTG CCCCCACCTC TCATCCTGCT GAGCCACGTG 
GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT CGAGCTCCTC ACGACCAAGA AGAGGGTGAC 
GTTGGATTAA AACTCTACCT CAGTAAGGAG GATCTGAAAA AACTTCATGA TTTTGAGGAG 
CAGTGCGTGG AAAAATACTT CCATGAGAAG ATGGAAGATG TGAATTGTAG TTGTGAGGAA 
CGAATCCGAG TGACATCAGA AAGGGTTACA GAGATGTACT TCCAGCTGAA AGAAATGAAT 
GAAAAGGTGT CTTTTATAAA GGACTCCTTA CTGTCTTTGG ACAGCCAGGT GGGACACCTG 
CAGGATCTCT CTGCCCTGAC TGTGGATACC CTGAAAGTCC TTTCTGCTGT TGACACTTTG 
CAAGAGGATG AGGCTCTCCT GGCCAAGAGA AAGCATTCTA CTTGCAAAAA ACTTCCCCAC 
AGCTGGAGCA ATGTCATCTG TGCAGAGGTT CTAGGCAGCA TGGAGATCGC TGGAGAGAAG 
AAATACCAGT ATTATAGCAT GCCCTCTTCT TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT 
CCCCCAAGAG TGCAGAGGGG GGCACTTCTT GAGATTACAA ACAGTAAAAG AGAGGCTACA 
AATGTAAGAA ATGACCAGGA AAGGCAAGAA ACACAAAGTA GTATAGTGGT TTCTGGGGTG 
TCTCCTAACA GGCAAGCACA CTCAAAGTAT GGCCAGTTTC TTCTGGTCCC CTCTAATCTA 
AAGCGAGTTC CTTTTTCAGC AGAAACTGTC TTGCCTCTGT CCAGACCCTC TGTGCCAGAT 
GTGCTGGCAA CTGAACAGGA CATCCAGACT GAGGTTCTTG TTCATCTGAC TGGGCAGACC 
CCAGTTGTCT CTGACTGGGC ATCAGTGGAT GAACCCAAGG AAAAGCACGA GCCTATTGCT 
CACTTACTGG ATGGACAAGA CAAGGCAGAG CAAGTGCTAC CCACTTTGAG TTGCACACCT 
GAACCCATGA CAATGAGCTC CCCTCTTTCC CAAGCCAAGA TCATGCAAAC TGGAGGTGGA 
TATGTAAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAT CAAGAAAAAG 
TGGCAAACCT GCTTGCCCTC CACTTGTGAC AGTGATTCCT CTCGGAGTGA ACAGCACCAG 
AAGCAGGCCC AGGACAGCTC CCTATCTGAT AACTCAACAA GATCGGCCCA GAGTAGTGAA 
TGCTCAGAGG TGGGACCATG GCTTCAGCCA AACACATCCT TTTGGATCAA TCCTCTCCGC 
AGATACAGGC CCTTCGCTAG GAGTCATAGT TTTAGATTCC ATAAGGAGGA GAAATTGATG 
AAGATCTGTA AGATTAAAAA TCTTTCAGGC TCTTCAGAAA TAGGGCAGGG AGCATGGGTC 
AAAGCGAAAA TGCTAACCAA AGACAGGAGA CTGTCAAAGA AAAAGAAGAA TACTCAAGGA 
CTCCAGGTGC CAATCATAAC AGTCAATGCC TGCTCTCAGA GTGACCAGTT GAATCCAGAG 
CCAGGAGAAA ACAGCATCTC TGAAGAGGAG TACAGCAAGA ACTGGTTCAC AGTGTCCAAA 
TTTAGTCACA CAGGTGTAGA ACCTTACATA CATCAGAAAA TGAAAACTAA AGAAATTGGA 
CAATGTGCTA TACAAATCAG TGATTACCTA AAGCAGTCTC AAGAGGATCT CAGCAAAAAC 
TCTTTGTGGA ATTCCAGGAG CACCAACCTC AATAGGAACT CCCTGCTGAA AAGTTCAATT 
GGAGTTGACA AGATCTCAGC CTCCTTAAAA AGCCCTCAAG AGCCTCACCA TCATTATTCA 
GCCATTGAAA GGAATAATTT AATGAGGCTT TCTCAGACCA TACCATTTAC ACCAGTCCAA 
CTGTTTGCAG GAGAAGAAAT AACTGTCTAC AGGTTGGAGG AGAGTTCCCC TTTAAACCTT 
GATAAAAGCA TGTCCTCTTG GTCTCAGCGT GGGAGAGCGG CAATGATCCA GGTATTGTCC 
CGAGAGGAGA TGGATGGGGG CCTCCGTAAA GCTATGAGAG TCGTCAGCAC TTGGTCTGAG 
GATGACATTC TCAAGCCGGG ACAAGTTTTC ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG 
CGGACATGGC ATAAAATCTT CCAGGAGAGC ACTGTGCTTC ATCTTTGCCT CAGGGAAATT 
CAACAACAAA GAGCTGCTCA AAAATTGATC TATACCTTCA ACCAAGTGAA ACCACAAACC 
ATACCCTACA CACCAAGGTT CCTGGAAGTT TTCTTAATCT ACTGCCATTC AGCCAACCAG 
TGGTTGACCA TTGAGAAGTA TATGACAGGG GAGTTCCGGA AGTATAACAA CAACAATGGT 
GATGAAATCA CCCCCACCAA CACCCTGGAG GAGCTGATGT TGGCTTTCTC TCACTGGACC 
TATGAGTACA CTCGGGGAGA GCTGCTGGTT TTAGATTTGC AAGGTGTTGG AGAAAATTTG 
ACAGATCCAT CTGTTATAAA ACCTGAAGTC AAACAATCAA GAGGAATGGT GTTTGGACCG 
GCCAATTTGG GGGAAGATGC AATTAGAAAC TTCATTGCAA AACATCATTG TAACTCCTGC 
TGCCGGAAGC TCAAACTCCC GGATTTAAAA AGAAATGACT ATTCCCCTGA AAGGATAAAT 
TCCACCTTTG GACTTGAGAT AAAAATAGAA TCAGCTGAGG AGCCTCCAGC AAGGGAGACG 
GGTAGAAATT CCCCAGAAGA TGATATGCAA CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516547.1
CDS	239..6214
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518245.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518245.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGGA AAAGTCTATA GTCCTTCATA AATCAAGGAA GAAGTCAAAA 
GAACAAAATG TATCAGATGA CCCTGAGTCT ACTGGCTTTC TTTACCCTTA CAATGACCTG 
CTGGTTTGGG CTGTGCTGAT GAAAAGGCAG AAGATGGCTA TGTTCTTCTG GCAGCATGGA 
GAGGAGGCCA CGGTTAAAGC CGTGATTGCG TGTATCCTCT ACCGGGCAAT GGCCCATGAA 
GCTAAGGAGA GTCACATGGT GGATGATGCC TCAGAAGAGT TGAAGAATTA CTCAAAACAG 
TTTGGCCAGC TGGCTCTGGA CTTGTTGGAG AAGGCATTCA AGCAGAATGA GCGCATGGCC 
ATGACGCTGT TGACGTATGA ACTCAGGAAC TGGAGCAATT CGACCTGCCT TAAACTGGCC 
GTGTCGGGAG GATTACGACC CTTTGTTTCA CATACTTGTA CCCAGATGCT ACTGACAGAC 
ATGTGGATGG GGAGGCTGAA AATGAGGAAA AACTCTTGGT TAAAGATTAT TATAAGCATT 
ATTTTACCAC CCACCATTTT GACACTGGAA TTTAAAAGCA AAGCTGAGAT GTCACATGTT 
CCCCAGTCCC AGGACTTCCA ATTTATGTGG TATTACAGTG ACCAGAACGC CAGCAGTTCC 
AAAGAAAGTG CTTCTGTGAA AGAGTATGAT TTGGAAAGGG GCCATGATGA GAAACTGGAT 
GAAAATCAGC ATTTTGGTTT GGAAAGTGGG CACCAACACC TTCCGTGGAC CAGGAAAGTC 
TATGAGTTCT ACAGTGCTCC AATTGTCAAG TTTTGGTTTT ATACGATGGC GTATTTGGCA 
TTCCTCATGC TGTTCACTTA CACCGTGTTG GTGGAGATGC AGCCCCAGCC CAGCGTGCAG 
GAGTGGCTTG TTAGCATTTA CATCTTCACC AATGCTATTG AGGTGGTCAG GGAGATCTGT 
ATTTCAGAAC CTGGGAAGTT TACCCAAAAG GTGAAGGTAT GGATTAGTGA GTACTGGAAC 
TTAACAGAAA CTGTGGCCAT TGGCCTGTTT TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC 
CCTCCTTTTC ACACAGCGGG AAGACTGATC TACTGCATAG ACATCATATT CTGGTTCTCA 
CGGCTCCTGG ACTTCTTTGC TGTGAATCAA CATGCAGGTC CATATGTGAC CATGATTGCA 
AAAATGACAG CAAACATGTT CTATATTGTG ATCATCATGG CCATAGTCCT GCTGAGCTTT 
GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA AAAGAGCCAC CATCTTGGAG TCTAGCTCGA 
GATATTGTAT TTGAGCCATA CTGGATGATA TACGGAGAAG TCTATGCTGG AGAAATAGAT 
GTTTGTTCAA GCCAGCCATC CTGCCCTCCT GGTTCTTTTC TTACTCCATT CTTGCAAGCT 
GTCTACCTCT TCGTGCAATA TATCATCATG GTGAACCTGT TGATTGCTTT CTTCAACAAC 
GTTTACTTAG ATATGGAATC CATTTCAAAT AACCTGTGGA AATACAACCG CTATCGCTAC 
ATCATGACCT ACCACGAGAA GCCCTGGCTG CCCCCACCTC TCATCCTGCT GAGCCACGTG 
GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT CGAGCTCCTC ACGACCAAGA AGAGGGTGAC 
GTTGGATTAA AACTCTACCT CAGTAAGGAG GATCTGAAAA AACTTCATGA TTTTGAGGAG 
CAGTGCGTGG AAAAATACTT CCATGAGAAG ATGGAAGATG TGAATTGTAG TTGTGAGGAA 
CGAATCCGAG TGACATCAGA AAGGGTTACA GAGATGTACT TCCAGCTGAA AGAAATGAAT 
GAAAAGGTGT CTTTTATAAA GGACTCCTTA CTGTCTTTGG ACAGCCAGGT GGGACACCTG 
CAGGATCTCT CTGCCCTGAC TGTGGATACC CTGAAAGTCC TTTCTGCTGT TGACACTTTG 
CAAGAGGATG AGGCTCTCCT GGCCAAGAGA AAGCATTCTA CTTGCAAAAA ACTTCCCCAC 
AGCTGGAGCA ATGTCATCTG TGCAGAGGTT CTAGGCAGCA TGGAGATCGC TGGAGAGAAG 
AAATACCAGT ATTATAGCAT GCCCTCTTCT TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT 
CCCCCAAGAG TGCAGAGGGG GGCACTTCTT GAGATTACAA ACAGTAAAAG AGAGGCTACA 
AATGTAAGAA ATGACCAGGA AAGGCAAGAA ACACAAAGTA GTATAGTGGT TTCTGGGGTG 
TCTCCTAACA GGCAAGCACA CTCAAAGTAT GGCCAGTTTC TTCTGGTCCC CTCTAATCTA 
AAGCGAGTTC CTTTTTCAGC AGAAACTGTC TTGCCTCTGT CCAGACCCTC TGTGCCAGAT 
GTGCTGGCAA CTGAACAGGA CATCCAGACT GAGGTTCTTG TTCATCTGAC TGGGCAGACC 
CCAGTTGTCT CTGACTGGGC ATCAGTGGAT GAACCCAAGG AAAAGCACGA GCCTATTGCT 
CACTTACTGG ATGGACAAGA CAAGGCAGAG CAAGTGCTAC CCACTTTGAG TTGCACACCT 
GAACCCATGA CAATGAGCTC CCCTCTTTCC CAAGCCAAGA TCATGCAAAC TGGAGGTGGA 
TATGTAAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAT CAAGAAAAAG 
TGGCAAACCT GCTTGCCCTC CACTTGTGAC AGTGATTCCT CTCGGAGTGA ACAGCACCAG 
AAGCAGGCCC AGGACAGCTC CCTATCTGAT AACTCAACAA GATCGGCCCA GAGTAGTGAA 
TGCTCAGAGG TGGGACCATG GCTTCAGCCA AACACATCCT TTTGGATCAA TCCTCTCCGC 
AGATACAGGC CCTTCGCTAG GAGTCATAGT TTTAGATTCC ATAAGGAGGA GAAATTGATG 
AAGATCTGTA AGATTAAAAA TCTTTCAGGC TCTTCAGAAA TAGGGCAGGG AGCATGGGTC 
AAAGCGAAAA TGCTAACCAA AGACAGGAGA CTGTCAAAGA AAAAGAAGAA TACTCAAGGA 
CTCCAGGTGC CAATCATAAC AGTCAATGCC TGCTCTCAGA GTGACCAGTT GAATCCAGAG 
CCAGGAGAAA ACAGCATCTC TGAAGAGGAG TACAGCAAGA ACTGGTTCAC AGTGTCCAAA 
TTTAGTCACA CAGGTGTAGA ACCTTACATA CATCAGAAAA TGAAAACTAA AGAAATTGGA 
CAATGTGCTA TACAAATCAG TGATTACCTA AAGCAGTCTC AAGAGGATCT CAGCAAAAAC 
TCTTTGTGGA ATTCCAGGAG CACCAACCTC AATAGGAACT CCCTGCTGAA AAGTTCAATT 
GGAGTTGACA AGATCTCAGC CTCCTTAAAA AGCCCTCAAG AGCCTCACCA TCATTATTCA 
GCCATTGAAA GGAATAATTT AATGAGGCTT TCTCAGACCA TACCATTTAC ACCAGTCCAA 
CTGTTTGCAG GAGAAGAAAT AACTGTCTAC AGGTTGGAGG AGAGTTCCCC TTTAAACCTT 
GATAAAAGCA TGTCCTCTTG GTCTCAGCGT GGGAGAGCGG CAATGATCCA GGTATTGTCC 
CGAGAGGAGA TGGATGGGGG CCTCCGTAAA GCTATGAGAG TCGTCAGCAC TTGGTCTGAG 
GATGACATTC TCAAGCCGGG ACAAGTTTTC ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG 
CGGACATGGC ATAAAATCTT CCAGGAGAGC ACTGTGCTTC ATCTTTGCCT CAGGGAAATT 
CAACAACAAA GAGCTGCTCA AAAATTGATC TATACCTTCA ACCAAGTGAA ACCACAAACC 
ATACCCTACA CACCAAGGTT CCTGGAAGTT TTCTTAATCT ACTGCCATTC AGCCAACCAG 
TGGTTGACCA TTGAGAAGTA TATGACAGGG GAGTTCCGGA AGTATAACAA CAACAATGGT 
GATGAAATCA CCCCCACCAA CACCCTGGAG GAGCTGATGT TGGCTTTCTC TCACTGGACC 
TATGAGTACA CTCGGGGAGA GCTGCTGGTT TTAGATTTGC AAGGTGTTGG AGAAAATTTG 
ACAGATCCAT CTGTTATAAA ACCTGAAGTC AAACAATCAA GAGGAATGGT GTTTGGACCG 
GCCAATTTGG GGGAAGATGC AATTAGAAAC TTCATTGCAA AACATCATTG TAACTCCTGC 
TGCCGGAAGC TCAAACTCCC GGATTTAAAA AGAAATGACT ATTCCCCTGA AAGGATAAAT 
TCCACCTTTG GACTTGAGAT AAAAATAGAA TCAGCTGAGG AGCCTCCAGC AAGGGAGACG 
GGTAGAAATT CCCCAGAAGA TGATATGCAA CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518246.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516548.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA.

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CloneID	OHu75941
Clone ID Related Accession (Same CDS sequence)	XM_011518246.1
Accession Version	XM_011518246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC 
TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG 
CGTGGGAGAG CGGCAATGAT CCAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC 
ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC 
TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT 
TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG 
GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG 
GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT 
TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC 
AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC 
TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA 
AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA 
TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516548.1
CDS	239..6184
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518246.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518246.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC 
TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG 
CGTGGGAGAG CGGCAATGAT CCAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC 
ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC 
TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT 
TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG 
GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG 
GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT 
TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC 
AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC 
TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA 
AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA 
TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518247.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516549.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X4, mRNA.

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CloneID	OHu75942
Clone ID Related Accession (Same CDS sequence)	XM_011518247.1
Accession Version	XM_011518247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG 
GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT 
GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC 
ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA 
GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC 
ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG 
CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA 
GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT 
TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC 
CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG 
TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA 
CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT 
CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG 
AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA 
GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG 
TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT 
TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA 
GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT 
TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC 
AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG 
AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT 
ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA 
AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG 
TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT 
CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT 
CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC 
AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG 
CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC 
AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT 
GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT 
TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA 
ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA 
TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA 
TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA 
GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA 
AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT 
CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC 
AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG 
AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG 
TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG 
AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT 
CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG 
ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG 
GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA 
GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG 
AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC 
AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG 
CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC 
TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA 
ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC 
CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG 
ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT 
TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA 
TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT 
GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT 
GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT 
GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516549.1
CDS	239..6178
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518247.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518247.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG 
GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT 
GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC 
ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA 
GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC 
ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG 
CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA 
GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT 
TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC 
CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG 
TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA 
CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT 
CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG 
AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA 
GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG 
TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT 
TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA 
GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT 
TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC 
AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG 
AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT 
ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA 
AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG 
TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT 
CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT 
CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC 
AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG 
CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC 
AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT 
GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT 
TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA 
ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA 
TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA 
TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA 
GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA 
AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT 
CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC 
AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG 
AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG 
TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG 
AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT 
CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG 
ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG 
GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA 
GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG 
AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC 
AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG 
CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC 
TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA 
ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC 
CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG 
ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT 
TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA 
TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT 
GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT 
GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT 
GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518248.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516550.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X5, mRNA.

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CloneID	OHu75943
Clone ID Related Accession (Same CDS sequence)	XM_011518248.1
Accession Version	XM_011518248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAC 
CAGAGACACT CCTCAGGAAA TAGAAATGAG TCTGCAGAAA GTACGCTGCA CTCCCAGTTC 
ATTAGAACTG CACAGCCATA CAAATTCAAG GAAAAGTCTA TAGTCCTTCA TAAATCAAGG 
AAGAAGTCAA AAGAACAAAA TGTATCAGAT GACCCTGAGT CTACTGGCTT TCTTTACCCT 
TACAATGACC TGCTGGTTTG GGCTGTGCTG ATGAAAAGGC AGAAGATGGC TATGTTCTTC 
TGGCAGCATG GAGAGGAGGC CACGGTTAAA GCCGTGATTG CGTGTATCCT CTACCGGGCA 
ATGGCCCATG AAGCTAAGGA GAGTCACATG GTGGATGATG CCTCAGAAGA GTTGAAGAAT 
TACTCAAAAC AGTTTGGCCA GCTGGCTCTG GACTTGTTGG AGAAGGCATT CAAGCAGAAT 
GAGCGCATGG CCATGACGCT GTTGACGTAT GAACTCAGGA ACTGGAGCAA TTCGACCTGC 
CTTAAACTGG CCGTGTCGGG AGGATTACGA CCCTTTGTTT CACATACTTG TACCCAGATG 
CTACTGACAG ACATGTGGAT GGGGAGGCTG AAAATGAGGA AAAACTCTTG GTTAAAGATT 
ATTATAAGCA TTATTTTACC ACCCACCATT TTGACACTGG AATTTAAAAG CAAAGCTGAG 
ATGTCACATG TTCCCCAGTC CCAGGACTTC CAATTTATGT GGTATTACAG TGACCAGAAC 
GCCAGCAGTT CCAAAGAAAG TGCTTCTGTG AAAGAGTATG ATTTGGAAAG GGGCCATGAT 
GAGAAACTGG ATGAAAATCA GCATTTTGGT TTGGAAAGTG GGCACCAACA CCTTCCGTGG 
ACCAGGAAAG TCTATGAGTT CTACAGTGCT CCAATTGTCA AGTTTTGGTT TTATACGATG 
GCGTATTTGG CATTCCTCAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG 
CCCAGCGTGC AGGAGTGGCT TGTTAGCATT TACATCTTCA CCAATGCTAT TGAGGTGGTC 
AGGGAGATCT GTATTTCAGA ACCTGGGAAG TTTACCCAAA AGGTGAAGGT ATGGATTAGT 
GAGTACTGGA ACTTAACAGA AACTGTGGCC ATTGGCCTGT TTTCAGCTGG CTTCGTCCTT 
CGATGGGGTG ACCCTCCTTT TCACACAGCG GGAAGACTGA TCTACTGCAT AGACATCATA 
TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCATATGTG 
ACCATGATTG CAAAAATGAC AGCAAACATG TTCTATATTG TGATCATCAT GGCCATAGTC 
CTGCTGAGCT TTGGAGTGGC ACGCAAGGCC ATCCTTTCGC CAAAAGAGCC ACCATCTTGG 
AGTCTAGCTC GAGATATTGT ATTTGAGCCA TACTGGATGA TATACGGAGA AGTCTATGCT 
GGAGAAATAG ATGTTTGTTC AAGCCAGCCA TCCTGCCCTC CTGGTTCTTT TCTTACTCCA 
TTCTTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT 
TTCTTCAACA ACGTTTACTT AGATATGGAA TCCATTTCAA ATAACCTGTG GAAATACAAC 
CGCTATCGCT ACATCATGAC CTACCACGAG AAGCCCTGGC TGCCCCCACC TCTCATCCTG 
CTGAGCCACG TGGGCCTTCT CCTCCGCCGC CTGTGCTGTC ATCGAGCTCC TCACGACCAA 
GAAGAGGGTG ACGTTGGATT AAAACTCTAC CTCAGTAAGG AGGATCTGAA AAAACTTCAT 
GATTTTGAGG AGCAGTGCGT GGAAAAATAC TTCCATGAGA AGATGGAAGA TGTGAATTGT 
AGTTGTGAGG AACGAATCCG AGTGACATCA GAAAGGGTTA CAGAGATGTA CTTCCAGCTG 
AAAGAAATGA ATGAAAAGGT GTCTTTTATA AAGGACTCCT TACTGTCTTT GGACAGCCAG 
GTGGGACACC TGCAGGATCT CTCTGCCCTG ACTGTGGATA CCCTGAAAGT CCTTTCTGCT 
GTTGACACTT TGCAAGAGGA TGAGGCTCTC CTGGCCAAGA GAAAGCATTC TACTTGCAAA 
AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTAGGCAG CATGGAGATC 
GCTGGAGAGA AGAAATACCA GTATTATAGC ATGCCCTCTT CTTTGCTGAG GAGCCTGGCT 
GGAGGCCGGC ATCCCCCAAG AGTGCAGAGG GGGGCACTTC TTGAGATTAC AAACAGTAAA 
AGAGAGGCTA CAAATGTAAG AAATGACCAG GAAAGGCAAG AAACACAAAG TAGTATAGTG 
GTTTCTGGGG TGTCTCCTAA CAGGCAAGCA CACTCAAAGT ATGGCCAGTT TCTTCTGGTC 
CCCTCTAATC TAAAGCGAGT TCCTTTTTCA GCAGAAACTG TCTTGCCTCT GTCCAGACCC 
TCTGTGCCAG ATGTGCTGGC AACTGAACAG GACATCCAGA CTGAGGTTCT TGTTCATCTG 
ACTGGGCAGA CCCCAGTTGT CTCTGACTGG GCATCAGTGG ATGAACCCAA GGAAAAGCAC 
GAGCCTATTG CTCACTTACT GGATGGACAA GACAAGGCAG AGCAAGTGCT ACCCACTTTG 
AGTTGCACAC CTGAACCCAT GACAATGAGC TCCCCTCTTT CCCAAGCCAA GATCATGCAA 
ACTGGAGGTG GATATGTAAA CTGGGCATTT TCAGAAGGTG ATGAAACTGG TGTGTTTAGC 
ATCAAGAAAA AGTGGCAAAC CTGCTTGCCC TCCACTTGTG ACAGTGATTC CTCTCGGAGT 
GAACAGCACC AGAAGCAGGC CCAGGACAGC TCCCTATCTG ATAACTCAAC AAGATCGGCC 
CAGAGTAGTG AATGCTCAGA GGTGGGACCA TGGCTTCAGC CAAACACATC CTTTTGGATC 
AATCCTCTCC GCAGATACAG GCCCTTCGCT AGGAGTCATA GTTTTAGATT CCATAAGGAG 
GAGAAATTGA TGAAGATCTG TAAGATTAAA AATCTTTCAG GCTCTTCAGA AATAGGGCAG 
GGAGCATGGG TCAAAGCGAA AATGCTAACC AAAGACAGGA GACTGTCAAA GAAAAAGAAG 
AATACTCAAG GACTCCAGGT GCCAATCATA ACAGTCAATG CCTGCTCTCA GAGTGACCAG 
TTGAATCCAG AGCCAGGAGA AAACAGCATC TCTGAAGAGG AGTACAGCAA GAACTGGTTC 
ACAGTGTCCA AATTTAGTCA CACAGGTGTA GAACCTTACA TACATCAGAA AATGAAAACT 
AAAGAAATTG GACAATGTGC TATACAAATC AGTGATTACC TAAAGCAGTC TCAAGAGGAT 
CTCAGCAAAA ACTCTTTGTG GAATTCCAGG AGCACCAACC TCAATAGGAA CTCCCTGCTG 
AAAAGTTCAA TTGGAGTTGA CAAGATCTCA GCCTCCTTAA AAAGCCCTCA AGAGCCTCAC 
CATCATTATT CAGCCATTGA AAGGAATAAT TTAATGAGGC TTTCTCAGAC CATACCATTT 
ACACCAGTCC AACTGTTTGC AGGAGAAGAA ATAACTGTCT ACAGGTTGGA GGAGAGTTCC 
CCTTTAAACC TTGATAAAAG CATGTCCTCT TGGTCTCAGC GTGGGAGAGC GGCAATGATC 
CAGGTATTGT CCCGAGAGGA GATGGATGGG GGCCTCCGTA AAGCTATGAG AGTCGTCAGC 
ACTTGGTCTG AGGATGACAT TCTCAAGCCG GGACAAGTTT TCATTGTCAA GTCCTTTCTT 
CCTGAGGTTG TGCGGACATG GCATAAAATC TTCCAGGAGA GCACTGTGCT TCATCTTTGC 
CTCAGGGAAA TTCAACAACA AAGAGCTGCT CAAAAATTGA TCTATACCTT CAACCAAGTG 
AAACCACAAA CCATACCCTA CACACCAAGG TTCCTGGAAG TTTTCTTAAT CTACTGCCAT 
TCAGCCAACC AGTGGTTGAC CATTGAGAAG TATATGACAG GGGAGTTCCG GAAGTATAAC 
AACAACAATG GTGATGAAAT CACCCCCACC AACACCCTGG AGGAGCTGAT GTTGGCTTTC 
TCTCACTGGA CCTATGAGTA CACTCGGGGA GAGCTGCTGG TTTTAGATTT GCAAGGTGTT 
GGAGAAAATT TGACAGATCC ATCTGTTATA AAACCTGAAG TCAAACAATC AAGAGGAATG 
GTGTTTGGAC CGGCCAATTT GGGGGAAGAT GCAATTAGAA ACTTCATTGC AAAACATCAT 
TGTAACTCCT GCTGCCGGAA GCTCAAACTC CCGGATTTAA AAAGAAATGA CTATTCCCCT 
GAAAGGATAA ATTCCACCTT TGGACTTGAG ATAAAAATAG AATCAGCTGA GGAGCCTCCA 
GCAAGGGAGA CGGGTAGAAA TTCCCCAGAA GATGATATGC AACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516550.1
CDS	239..6166
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518248.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518248.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAC 
CAGAGACACT CCTCAGGAAA TAGAAATGAG TCTGCAGAAA GTACGCTGCA CTCCCAGTTC 
ATTAGAACTG CACAGCCATA CAAATTCAAG GAAAAGTCTA TAGTCCTTCA TAAATCAAGG 
AAGAAGTCAA AAGAACAAAA TGTATCAGAT GACCCTGAGT CTACTGGCTT TCTTTACCCT 
TACAATGACC TGCTGGTTTG GGCTGTGCTG ATGAAAAGGC AGAAGATGGC TATGTTCTTC 
TGGCAGCATG GAGAGGAGGC CACGGTTAAA GCCGTGATTG CGTGTATCCT CTACCGGGCA 
ATGGCCCATG AAGCTAAGGA GAGTCACATG GTGGATGATG CCTCAGAAGA GTTGAAGAAT 
TACTCAAAAC AGTTTGGCCA GCTGGCTCTG GACTTGTTGG AGAAGGCATT CAAGCAGAAT 
GAGCGCATGG CCATGACGCT GTTGACGTAT GAACTCAGGA ACTGGAGCAA TTCGACCTGC 
CTTAAACTGG CCGTGTCGGG AGGATTACGA CCCTTTGTTT CACATACTTG TACCCAGATG 
CTACTGACAG ACATGTGGAT GGGGAGGCTG AAAATGAGGA AAAACTCTTG GTTAAAGATT 
ATTATAAGCA TTATTTTACC ACCCACCATT TTGACACTGG AATTTAAAAG CAAAGCTGAG 
ATGTCACATG TTCCCCAGTC CCAGGACTTC CAATTTATGT GGTATTACAG TGACCAGAAC 
GCCAGCAGTT CCAAAGAAAG TGCTTCTGTG AAAGAGTATG ATTTGGAAAG GGGCCATGAT 
GAGAAACTGG ATGAAAATCA GCATTTTGGT TTGGAAAGTG GGCACCAACA CCTTCCGTGG 
ACCAGGAAAG TCTATGAGTT CTACAGTGCT CCAATTGTCA AGTTTTGGTT TTATACGATG 
GCGTATTTGG CATTCCTCAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG 
CCCAGCGTGC AGGAGTGGCT TGTTAGCATT TACATCTTCA CCAATGCTAT TGAGGTGGTC 
AGGGAGATCT GTATTTCAGA ACCTGGGAAG TTTACCCAAA AGGTGAAGGT ATGGATTAGT 
GAGTACTGGA ACTTAACAGA AACTGTGGCC ATTGGCCTGT TTTCAGCTGG CTTCGTCCTT 
CGATGGGGTG ACCCTCCTTT TCACACAGCG GGAAGACTGA TCTACTGCAT AGACATCATA 
TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCATATGTG 
ACCATGATTG CAAAAATGAC AGCAAACATG TTCTATATTG TGATCATCAT GGCCATAGTC 
CTGCTGAGCT TTGGAGTGGC ACGCAAGGCC ATCCTTTCGC CAAAAGAGCC ACCATCTTGG 
AGTCTAGCTC GAGATATTGT ATTTGAGCCA TACTGGATGA TATACGGAGA AGTCTATGCT 
GGAGAAATAG ATGTTTGTTC AAGCCAGCCA TCCTGCCCTC CTGGTTCTTT TCTTACTCCA 
TTCTTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT 
TTCTTCAACA ACGTTTACTT AGATATGGAA TCCATTTCAA ATAACCTGTG GAAATACAAC 
CGCTATCGCT ACATCATGAC CTACCACGAG AAGCCCTGGC TGCCCCCACC TCTCATCCTG 
CTGAGCCACG TGGGCCTTCT CCTCCGCCGC CTGTGCTGTC ATCGAGCTCC TCACGACCAA 
GAAGAGGGTG ACGTTGGATT AAAACTCTAC CTCAGTAAGG AGGATCTGAA AAAACTTCAT 
GATTTTGAGG AGCAGTGCGT GGAAAAATAC TTCCATGAGA AGATGGAAGA TGTGAATTGT 
AGTTGTGAGG AACGAATCCG AGTGACATCA GAAAGGGTTA CAGAGATGTA CTTCCAGCTG 
AAAGAAATGA ATGAAAAGGT GTCTTTTATA AAGGACTCCT TACTGTCTTT GGACAGCCAG 
GTGGGACACC TGCAGGATCT CTCTGCCCTG ACTGTGGATA CCCTGAAAGT CCTTTCTGCT 
GTTGACACTT TGCAAGAGGA TGAGGCTCTC CTGGCCAAGA GAAAGCATTC TACTTGCAAA 
AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTAGGCAG CATGGAGATC 
GCTGGAGAGA AGAAATACCA GTATTATAGC ATGCCCTCTT CTTTGCTGAG GAGCCTGGCT 
GGAGGCCGGC ATCCCCCAAG AGTGCAGAGG GGGGCACTTC TTGAGATTAC AAACAGTAAA 
AGAGAGGCTA CAAATGTAAG AAATGACCAG GAAAGGCAAG AAACACAAAG TAGTATAGTG 
GTTTCTGGGG TGTCTCCTAA CAGGCAAGCA CACTCAAAGT ATGGCCAGTT TCTTCTGGTC 
CCCTCTAATC TAAAGCGAGT TCCTTTTTCA GCAGAAACTG TCTTGCCTCT GTCCAGACCC 
TCTGTGCCAG ATGTGCTGGC AACTGAACAG GACATCCAGA CTGAGGTTCT TGTTCATCTG 
ACTGGGCAGA CCCCAGTTGT CTCTGACTGG GCATCAGTGG ATGAACCCAA GGAAAAGCAC 
GAGCCTATTG CTCACTTACT GGATGGACAA GACAAGGCAG AGCAAGTGCT ACCCACTTTG 
AGTTGCACAC CTGAACCCAT GACAATGAGC TCCCCTCTTT CCCAAGCCAA GATCATGCAA 
ACTGGAGGTG GATATGTAAA CTGGGCATTT TCAGAAGGTG ATGAAACTGG TGTGTTTAGC 
ATCAAGAAAA AGTGGCAAAC CTGCTTGCCC TCCACTTGTG ACAGTGATTC CTCTCGGAGT 
GAACAGCACC AGAAGCAGGC CCAGGACAGC TCCCTATCTG ATAACTCAAC AAGATCGGCC 
CAGAGTAGTG AATGCTCAGA GGTGGGACCA TGGCTTCAGC CAAACACATC CTTTTGGATC 
AATCCTCTCC GCAGATACAG GCCCTTCGCT AGGAGTCATA GTTTTAGATT CCATAAGGAG 
GAGAAATTGA TGAAGATCTG TAAGATTAAA AATCTTTCAG GCTCTTCAGA AATAGGGCAG 
GGAGCATGGG TCAAAGCGAA AATGCTAACC AAAGACAGGA GACTGTCAAA GAAAAAGAAG 
AATACTCAAG GACTCCAGGT GCCAATCATA ACAGTCAATG CCTGCTCTCA GAGTGACCAG 
TTGAATCCAG AGCCAGGAGA AAACAGCATC TCTGAAGAGG AGTACAGCAA GAACTGGTTC 
ACAGTGTCCA AATTTAGTCA CACAGGTGTA GAACCTTACA TACATCAGAA AATGAAAACT 
AAAGAAATTG GACAATGTGC TATACAAATC AGTGATTACC TAAAGCAGTC TCAAGAGGAT 
CTCAGCAAAA ACTCTTTGTG GAATTCCAGG AGCACCAACC TCAATAGGAA CTCCCTGCTG 
AAAAGTTCAA TTGGAGTTGA CAAGATCTCA GCCTCCTTAA AAAGCCCTCA AGAGCCTCAC 
CATCATTATT CAGCCATTGA AAGGAATAAT TTAATGAGGC TTTCTCAGAC CATACCATTT 
ACACCAGTCC AACTGTTTGC AGGAGAAGAA ATAACTGTCT ACAGGTTGGA GGAGAGTTCC 
CCTTTAAACC TTGATAAAAG CATGTCCTCT TGGTCTCAGC GTGGGAGAGC GGCAATGATC 
CAGGTATTGT CCCGAGAGGA GATGGATGGG GGCCTCCGTA AAGCTATGAG AGTCGTCAGC 
ACTTGGTCTG AGGATGACAT TCTCAAGCCG GGACAAGTTT TCATTGTCAA GTCCTTTCTT 
CCTGAGGTTG TGCGGACATG GCATAAAATC TTCCAGGAGA GCACTGTGCT TCATCTTTGC 
CTCAGGGAAA TTCAACAACA AAGAGCTGCT CAAAAATTGA TCTATACCTT CAACCAAGTG 
AAACCACAAA CCATACCCTA CACACCAAGG TTCCTGGAAG TTTTCTTAAT CTACTGCCAT 
TCAGCCAACC AGTGGTTGAC CATTGAGAAG TATATGACAG GGGAGTTCCG GAAGTATAAC 
AACAACAATG GTGATGAAAT CACCCCCACC AACACCCTGG AGGAGCTGAT GTTGGCTTTC 
TCTCACTGGA CCTATGAGTA CACTCGGGGA GAGCTGCTGG TTTTAGATTT GCAAGGTGTT 
GGAGAAAATT TGACAGATCC ATCTGTTATA AAACCTGAAG TCAAACAATC AAGAGGAATG 
GTGTTTGGAC CGGCCAATTT GGGGGAAGAT GCAATTAGAA ACTTCATTGC AAAACATCAT 
TGTAACTCCT GCTGCCGGAA GCTCAAACTC CCGGATTTAA AAAGAAATGA CTATTCCCCT 
GAAAGGATAA ATTCCACCTT TGGACTTGAG ATAAAAATAG AATCAGCTGA GGAGCCTCCA 
GCAAGGGAGA CGGGTAGAAA TTCCCCAGAA GATGATATGC AACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518249.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516551.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X6, mRNA.

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CloneID	OHu75944
Clone ID Related Accession (Same CDS sequence)	XM_011518249.1
Accession Version	XM_011518249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA 
AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC 
CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG 
AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC 
GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG 
GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC 
TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA 
CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC 
TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA 
ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG 
ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA 
TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA 
GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG 
GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA 
ATTGTCAAGT TTTGGTTTTA TACGATGGCG TATTTGGCAT TCCTCATGCT GTTCACTTAC 
ACCGTGTTGG TGGAGATGCA GCCCCAGCCC AGCGTGCAGG AGTGGCTTGT TAGCATTTAC 
ATCTTCACCA ATGCTATTGA GGTGGTCAGG GAGATCTGTA TTTCAGAACC TGGGAAGTTT 
ACCCAAAAGG TGAAGGTATG GATTAGTGAG TACTGGAACT TAACAGAAAC TGTGGCCATT 
GGCCTGTTTT CAGCTGGCTT CGTCCTTCGA TGGGGTGACC CTCCTTTTCA CACAGCGGGA 
AGACTGATCT ACTGCATAGA CATCATATTC TGGTTCTCAC GGCTCCTGGA CTTCTTTGCT 
GTGAATCAAC ATGCAGGTCC ATATGTGACC ATGATTGCAA AAATGACAGC AAACATGTTC 
TATATTGTGA TCATCATGGC CATAGTCCTG CTGAGCTTTG GAGTGGCACG CAAGGCCATC 
CTTTCGCCAA AAGAGCCACC ATCTTGGAGT CTAGCTCGAG ATATTGTATT TGAGCCATAC 
TGGATGATAT ACGGAGAAGT CTATGCTGGA GAAATAGATG TTTGTTCAAG CCAGCCATCC 
TGCCCTCCTG GTTCTTTTCT TACTCCATTC TTGCAAGCTG TCTACCTCTT CGTGCAATAT 
ATCATCATGG TGAACCTGTT GATTGCTTTC TTCAACAACG TTTACTTAGA TATGGAATCC 
ATTTCAAATA ACCTGTGGAA ATACAACCGC TATCGCTACA TCATGACCTA CCACGAGAAG 
CCCTGGCTGC CCCCACCTCT CATCCTGCTG AGCCACGTGG GCCTTCTCCT CCGCCGCCTG 
TGCTGTCATC GAGCTCCTCA CGACCAAGAA GAGGGTGACG TTGGATTAAA ACTCTACCTC 
AGTAAGGAGG ATCTGAAAAA ACTTCATGAT TTTGAGGAGC AGTGCGTGGA AAAATACTTC 
CATGAGAAGA TGGAAGATGT GAATTGTAGT TGTGAGGAAC GAATCCGAGT GACATCAGAA 
AGGGTTACAG AGATGTACTT CCAGCTGAAA GAAATGAATG AAAAGGTGTC TTTTATAAAG 
GACTCCTTAC TGTCTTTGGA CAGCCAGGTG GGACACCTGC AGGATCTCTC TGCCCTGACT 
GTGGATACCC TGAAAGTCCT TTCTGCTGTT GACACTTTGC AAGAGGATGA GGCTCTCCTG 
GCCAAGAGAA AGCATTCTAC TTGCAAAAAA CTTCCCCACA GCTGGAGCAA TGTCATCTGT 
GCAGAGGTTC TAGGCAGCAT GGAGATCGCT GGAGAGAAGA AATACCAGTA TTATAGCATG 
CCCTCTTCTT TGCTGAGGAG CCTGGCTGGA GGCCGGCATC CCCCAAGAGT GCAGAGGGGG 
GCACTTCTTG AGATTACAAA CAGTAAAAGA GAGGCTACAA ATGTAAGAAA TGACCAGGAA 
AGGCAAGAAA CACAAAGTAG TATAGTGGTT TCTGGGGTGT CTCCTAACAG GCAAGCACAC 
TCAAAGTATG GCCAGTTTCT TCTGGTCCCC TCTAATCTAA AGCGAGTTCC TTTTTCAGCA 
GAAACTGTCT TGCCTCTGTC CAGACCCTCT GTGCCAGATG TGCTGGCAAC TGAACAGGAC 
ATCCAGACTG AGGTTCTTGT TCATCTGACT GGGCAGACCC CAGTTGTCTC TGACTGGGCA 
TCAGTGGATG AACCCAAGGA AAAGCACGAG CCTATTGCTC ACTTACTGGA TGGACAAGAC 
AAGGCAGAGC AAGTGCTACC CACTTTGAGT TGCACACCTG AACCCATGAC AATGAGCTCC 
CCTCTTTCCC AAGCCAAGAT CATGCAAACT GGAGGTGGAT ATGTAAACTG GGCATTTTCA 
GAAGGTGATG AAACTGGTGT GTTTAGCATC AAGAAAAAGT GGCAAACCTG CTTGCCCTCC 
ACTTGTGACA GTGATTCCTC TCGGAGTGAA CAGCACCAGA AGCAGGCCCA GGACAGCTCC 
CTATCTGATA ACTCAACAAG ATCGGCCCAG AGTAGTGAAT GCTCAGAGGT GGGACCATGG 
CTTCAGCCAA ACACATCCTT TTGGATCAAT CCTCTCCGCA GATACAGGCC CTTCGCTAGG 
AGTCATAGTT TTAGATTCCA TAAGGAGGAG AAATTGATGA AGATCTGTAA GATTAAAAAT 
CTTTCAGGCT CTTCAGAAAT AGGGCAGGGA GCATGGGTCA AAGCGAAAAT GCTAACCAAA 
GACAGGAGAC TGTCAAAGAA AAAGAAGAAT ACTCAAGGAC TCCAGGTGCC AATCATAACA 
GTCAATGCCT GCTCTCAGAG TGACCAGTTG AATCCAGAGC CAGGAGAAAA CAGCATCTCT 
GAAGAGGAGT ACAGCAAGAA CTGGTTCACA GTGTCCAAAT TTAGTCACAC AGGTGTAGAA 
CCTTACATAC ATCAGAAAAT GAAAACTAAA GAAATTGGAC AATGTGCTAT ACAAATCAGT 
GATTACCTAA AGCAGTCTCA AGAGGATCTC AGCAAAAACT CTTTGTGGAA TTCCAGGAGC 
ACCAACCTCA ATAGGAACTC CCTGCTGAAA AGTTCAATTG GAGTTGACAA GATCTCAGCC 
TCCTTAAAAA GCCCTCAAGA GCCTCACCAT CATTATTCAG CCATTGAAAG GAATAATTTA 
ATGAGGCTTT CTCAGACCAT ACCATTTACA CCAGTCCAAC TGTTTGCAGG AGAAGAAATA 
ACTGTCTACA GGTTGGAGGA GAGTTCCCCT TTAAACCTTG ATAAAAGCAT GTCCTCTTGG 
TCTCAGCGTG GGAGAGCGGC AATGATCCAG GTATTGTCCC GAGAGGAGAT GGATGGGGGC 
CTCCGTAAAG CTATGAGAGT CGTCAGCACT TGGTCTGAGG ATGACATTCT CAAGCCGGGA 
CAAGTTTTCA TTGTCAAGTC CTTTCTTCCT GAGGTTGTGC GGACATGGCA TAAAATCTTC 
CAGGAGAGCA CTGTGCTTCA TCTTTGCCTC AGGGAAATTC AACAACAAAG AGCTGCTCAA 
AAATTGATCT ATACCTTCAA CCAAGTGAAA CCACAAACCA TACCCTACAC ACCAAGGTTC 
CTGGAAGTTT TCTTAATCTA CTGCCATTCA GCCAACCAGT GGTTGACCAT TGAGAAGTAT 
ATGACAGGGG AGTTCCGGAA GTATAACAAC AACAATGGTG ATGAAATCAC CCCCACCAAC 
ACCCTGGAGG AGCTGATGTT GGCTTTCTCT CACTGGACCT ATGAGTACAC TCGGGGAGAG 
CTGCTGGTTT TAGATTTGCA AGGTGTTGGA GAAAATTTGA CAGATCCATC TGTTATAAAA 
CCTGAAGTCA AACAATCAAG AGGAATGGTG TTTGGACCGG CCAATTTGGG GGAAGATGCA 
ATTAGAAACT TCATTGCAAA ACATCATTGT AACTCCTGCT GCCGGAAGCT CAAACTCCCG 
GATTTAAAAA GAAATGACTA TTCCCCTGAA AGGATAAATT CCACCTTTGG ACTTGAGATA 
AAAATAGAAT CAGCTGAGGA GCCTCCAGCA AGGGAGACGG GTAGAAATTC CCCAGAAGAT 
GATATGCAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516551.1
CDS	239..6073
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518249.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518249.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA 
AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC 
CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG 
AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC 
GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG 
GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC 
TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA 
CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC 
TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA 
ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG 
ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA 
TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA 
GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG 
GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA 
ATTGTCAAGT TTTGGTTTTA TACGATGGCG TATTTGGCAT TCCTCATGCT GTTCACTTAC 
ACCGTGTTGG TGGAGATGCA GCCCCAGCCC AGCGTGCAGG AGTGGCTTGT TAGCATTTAC 
ATCTTCACCA ATGCTATTGA GGTGGTCAGG GAGATCTGTA TTTCAGAACC TGGGAAGTTT 
ACCCAAAAGG TGAAGGTATG GATTAGTGAG TACTGGAACT TAACAGAAAC TGTGGCCATT 
GGCCTGTTTT CAGCTGGCTT CGTCCTTCGA TGGGGTGACC CTCCTTTTCA CACAGCGGGA 
AGACTGATCT ACTGCATAGA CATCATATTC TGGTTCTCAC GGCTCCTGGA CTTCTTTGCT 
GTGAATCAAC ATGCAGGTCC ATATGTGACC ATGATTGCAA AAATGACAGC AAACATGTTC 
TATATTGTGA TCATCATGGC CATAGTCCTG CTGAGCTTTG GAGTGGCACG CAAGGCCATC 
CTTTCGCCAA AAGAGCCACC ATCTTGGAGT CTAGCTCGAG ATATTGTATT TGAGCCATAC 
TGGATGATAT ACGGAGAAGT CTATGCTGGA GAAATAGATG TTTGTTCAAG CCAGCCATCC 
TGCCCTCCTG GTTCTTTTCT TACTCCATTC TTGCAAGCTG TCTACCTCTT CGTGCAATAT 
ATCATCATGG TGAACCTGTT GATTGCTTTC TTCAACAACG TTTACTTAGA TATGGAATCC 
ATTTCAAATA ACCTGTGGAA ATACAACCGC TATCGCTACA TCATGACCTA CCACGAGAAG 
CCCTGGCTGC CCCCACCTCT CATCCTGCTG AGCCACGTGG GCCTTCTCCT CCGCCGCCTG 
TGCTGTCATC GAGCTCCTCA CGACCAAGAA GAGGGTGACG TTGGATTAAA ACTCTACCTC 
AGTAAGGAGG ATCTGAAAAA ACTTCATGAT TTTGAGGAGC AGTGCGTGGA AAAATACTTC 
CATGAGAAGA TGGAAGATGT GAATTGTAGT TGTGAGGAAC GAATCCGAGT GACATCAGAA 
AGGGTTACAG AGATGTACTT CCAGCTGAAA GAAATGAATG AAAAGGTGTC TTTTATAAAG 
GACTCCTTAC TGTCTTTGGA CAGCCAGGTG GGACACCTGC AGGATCTCTC TGCCCTGACT 
GTGGATACCC TGAAAGTCCT TTCTGCTGTT GACACTTTGC AAGAGGATGA GGCTCTCCTG 
GCCAAGAGAA AGCATTCTAC TTGCAAAAAA CTTCCCCACA GCTGGAGCAA TGTCATCTGT 
GCAGAGGTTC TAGGCAGCAT GGAGATCGCT GGAGAGAAGA AATACCAGTA TTATAGCATG 
CCCTCTTCTT TGCTGAGGAG CCTGGCTGGA GGCCGGCATC CCCCAAGAGT GCAGAGGGGG 
GCACTTCTTG AGATTACAAA CAGTAAAAGA GAGGCTACAA ATGTAAGAAA TGACCAGGAA 
AGGCAAGAAA CACAAAGTAG TATAGTGGTT TCTGGGGTGT CTCCTAACAG GCAAGCACAC 
TCAAAGTATG GCCAGTTTCT TCTGGTCCCC TCTAATCTAA AGCGAGTTCC TTTTTCAGCA 
GAAACTGTCT TGCCTCTGTC CAGACCCTCT GTGCCAGATG TGCTGGCAAC TGAACAGGAC 
ATCCAGACTG AGGTTCTTGT TCATCTGACT GGGCAGACCC CAGTTGTCTC TGACTGGGCA 
TCAGTGGATG AACCCAAGGA AAAGCACGAG CCTATTGCTC ACTTACTGGA TGGACAAGAC 
AAGGCAGAGC AAGTGCTACC CACTTTGAGT TGCACACCTG AACCCATGAC AATGAGCTCC 
CCTCTTTCCC AAGCCAAGAT CATGCAAACT GGAGGTGGAT ATGTAAACTG GGCATTTTCA 
GAAGGTGATG AAACTGGTGT GTTTAGCATC AAGAAAAAGT GGCAAACCTG CTTGCCCTCC 
ACTTGTGACA GTGATTCCTC TCGGAGTGAA CAGCACCAGA AGCAGGCCCA GGACAGCTCC 
CTATCTGATA ACTCAACAAG ATCGGCCCAG AGTAGTGAAT GCTCAGAGGT GGGACCATGG 
CTTCAGCCAA ACACATCCTT TTGGATCAAT CCTCTCCGCA GATACAGGCC CTTCGCTAGG 
AGTCATAGTT TTAGATTCCA TAAGGAGGAG AAATTGATGA AGATCTGTAA GATTAAAAAT 
CTTTCAGGCT CTTCAGAAAT AGGGCAGGGA GCATGGGTCA AAGCGAAAAT GCTAACCAAA 
GACAGGAGAC TGTCAAAGAA AAAGAAGAAT ACTCAAGGAC TCCAGGTGCC AATCATAACA 
GTCAATGCCT GCTCTCAGAG TGACCAGTTG AATCCAGAGC CAGGAGAAAA CAGCATCTCT 
GAAGAGGAGT ACAGCAAGAA CTGGTTCACA GTGTCCAAAT TTAGTCACAC AGGTGTAGAA 
CCTTACATAC ATCAGAAAAT GAAAACTAAA GAAATTGGAC AATGTGCTAT ACAAATCAGT 
GATTACCTAA AGCAGTCTCA AGAGGATCTC AGCAAAAACT CTTTGTGGAA TTCCAGGAGC 
ACCAACCTCA ATAGGAACTC CCTGCTGAAA AGTTCAATTG GAGTTGACAA GATCTCAGCC 
TCCTTAAAAA GCCCTCAAGA GCCTCACCAT CATTATTCAG CCATTGAAAG GAATAATTTA 
ATGAGGCTTT CTCAGACCAT ACCATTTACA CCAGTCCAAC TGTTTGCAGG AGAAGAAATA 
ACTGTCTACA GGTTGGAGGA GAGTTCCCCT TTAAACCTTG ATAAAAGCAT GTCCTCTTGG 
TCTCAGCGTG GGAGAGCGGC AATGATCCAG GTATTGTCCC GAGAGGAGAT GGATGGGGGC 
CTCCGTAAAG CTATGAGAGT CGTCAGCACT TGGTCTGAGG ATGACATTCT CAAGCCGGGA 
CAAGTTTTCA TTGTCAAGTC CTTTCTTCCT GAGGTTGTGC GGACATGGCA TAAAATCTTC 
CAGGAGAGCA CTGTGCTTCA TCTTTGCCTC AGGGAAATTC AACAACAAAG AGCTGCTCAA 
AAATTGATCT ATACCTTCAA CCAAGTGAAA CCACAAACCA TACCCTACAC ACCAAGGTTC 
CTGGAAGTTT TCTTAATCTA CTGCCATTCA GCCAACCAGT GGTTGACCAT TGAGAAGTAT 
ATGACAGGGG AGTTCCGGAA GTATAACAAC AACAATGGTG ATGAAATCAC CCCCACCAAC 
ACCCTGGAGG AGCTGATGTT GGCTTTCTCT CACTGGACCT ATGAGTACAC TCGGGGAGAG 
CTGCTGGTTT TAGATTTGCA AGGTGTTGGA GAAAATTTGA CAGATCCATC TGTTATAAAA 
CCTGAAGTCA AACAATCAAG AGGAATGGTG TTTGGACCGG CCAATTTGGG GGAAGATGCA 
ATTAGAAACT TCATTGCAAA ACATCATTGT AACTCCTGCT GCCGGAAGCT CAAACTCCCG 
GATTTAAAAA GAAATGACTA TTCCCCTGAA AGGATAAATT CCACCTTTGG ACTTGAGATA 
AAAATAGAAT CAGCTGAGGA GCCTCCAGCA AGGGAGACGG GTAGAAATTC CCCAGAAGAT 
GATATGCAAC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518250.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516552.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X8, mRNA.

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CloneID	OHu75945
Clone ID Related Accession (Same CDS sequence)	XM_011518250.1
Accession Version	XM_011518250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GATCTGTATT 
TCAGAACCTG GGAAGTTTAC CCAAAAGGTG AAGGTATGGA TTAGTGAGTA CTGGAACTTA 
ACAGAAACTG TGGCCATTGG CCTGTTTTCA GCTGGCTTCG TCCTTCGATG GGGTGACCCT 
CCTTTTCACA CAGCGGGAAG ACTGATCTAC TGCATAGACA TCATATTCTG GTTCTCACGG 
CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCAT ATGTGACCAT GATTGCAAAA 
ATGACAGCAA ACATGTTCTA TATTGTGATC ATCATGGCCA TAGTCCTGCT GAGCTTTGGA 
GTGGCACGCA AGGCCATCCT TTCGCCAAAA GAGCCACCAT CTTGGAGTCT AGCTCGAGAT 
ATTGTATTTG AGCCATACTG GATGATATAC GGAGAAGTCT ATGCTGGAGA AATAGATGTT 
TGTTCAAGCC AGCCATCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCTT GCAAGCTGTC 
TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT CAACAACGTT 
TACTTAGATA TGGAATCCAT TTCAAATAAC CTGTGGAAAT ACAACCGCTA TCGCTACATC 
ATGACCTACC ACGAGAAGCC CTGGCTGCCC CCACCTCTCA TCCTGCTGAG CCACGTGGGC 
CTTCTCCTCC GCCGCCTGTG CTGTCATCGA GCTCCTCACG ACCAAGAAGA GGGTGACGTT 
GGATTAAAAC TCTACCTCAG TAAGGAGGAT CTGAAAAAAC TTCATGATTT TGAGGAGCAG 
TGCGTGGAAA AATACTTCCA TGAGAAGATG GAAGATGTGA ATTGTAGTTG TGAGGAACGA 
ATCCGAGTGA CATCAGAAAG GGTTACAGAG ATGTACTTCC AGCTGAAAGA AATGAATGAA 
AAGGTGTCTT TTATAAAGGA CTCCTTACTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG 
GATCTCTCTG CCCTGACTGT GGATACCCTG AAAGTCCTTT CTGCTGTTGA CACTTTGCAA 
GAGGATGAGG CTCTCCTGGC CAAGAGAAAG CATTCTACTT GCAAAAAACT TCCCCACAGC 
TGGAGCAATG TCATCTGTGC AGAGGTTCTA GGCAGCATGG AGATCGCTGG AGAGAAGAAA 
TACCAGTATT ATAGCATGCC CTCTTCTTTG CTGAGGAGCC TGGCTGGAGG CCGGCATCCC 
CCAAGAGTGC AGAGGGGGGC ACTTCTTGAG ATTACAAACA GTAAAAGAGA GGCTACAAAT 
GTAAGAAATG ACCAGGAAAG GCAAGAAACA CAAAGTAGTA TAGTGGTTTC TGGGGTGTCT 
CCTAACAGGC AAGCACACTC AAAGTATGGC CAGTTTCTTC TGGTCCCCTC TAATCTAAAG 
CGAGTTCCTT TTTCAGCAGA AACTGTCTTG CCTCTGTCCA GACCCTCTGT GCCAGATGTG 
CTGGCAACTG AACAGGACAT CCAGACTGAG GTTCTTGTTC ATCTGACTGG GCAGACCCCA 
GTTGTCTCTG ACTGGGCATC AGTGGATGAA CCCAAGGAAA AGCACGAGCC TATTGCTCAC 
TTACTGGATG GACAAGACAA GGCAGAGCAA GTGCTACCCA CTTTGAGTTG CACACCTGAA 
CCCATGACAA TGAGCTCCCC TCTTTCCCAA GCCAAGATCA TGCAAACTGG AGGTGGATAT 
GTAAACTGGG CATTTTCAGA AGGTGATGAA ACTGGTGTGT TTAGCATCAA GAAAAAGTGG 
CAAACCTGCT TGCCCTCCAC TTGTGACAGT GATTCCTCTC GGAGTGAACA GCACCAGAAG 
CAGGCCCAGG ACAGCTCCCT ATCTGATAAC TCAACAAGAT CGGCCCAGAG TAGTGAATGC 
TCAGAGGTGG GACCATGGCT TCAGCCAAAC ACATCCTTTT GGATCAATCC TCTCCGCAGA 
TACAGGCCCT TCGCTAGGAG TCATAGTTTT AGATTCCATA AGGAGGAGAA ATTGATGAAG 
ATCTGTAAGA TTAAAAATCT TTCAGGCTCT TCAGAAATAG GGCAGGGAGC ATGGGTCAAA 
GCGAAAATGC TAACCAAAGA CAGGAGACTG TCAAAGAAAA AGAAGAATAC TCAAGGACTC 
CAGGTGCCAA TCATAACAGT CAATGCCTGC TCTCAGAGTG ACCAGTTGAA TCCAGAGCCA 
GGAGAAAACA GCATCTCTGA AGAGGAGTAC AGCAAGAACT GGTTCACAGT GTCCAAATTT 
AGTCACACAG GTGTAGAACC TTACATACAT CAGAAAATGA AAACTAAAGA AATTGGACAA 
TGTGCTATAC AAATCAGTGA TTACCTAAAG CAGTCTCAAG AGGATCTCAG CAAAAACTCT 
TTGTGGAATT CCAGGAGCAC CAACCTCAAT AGGAACTCCC TGCTGAAAAG TTCAATTGGA 
GTTGACAAGA TCTCAGCCTC CTTAAAAAGC CCTCAAGAGC CTCACCATCA TTATTCAGCC 
ATTGAAAGGA ATAATTTAAT GAGGCTTTCT CAGACCATAC CATTTACACC AGTCCAACTG 
TTTGCAGGAG AAGAAATAAC TGTCTACAGG TTGGAGGAGA GTTCCCCTTT AAACCTTGAT 
AAAAGCATGT CCTCTTGGTC TCAGCGTGGG AGAGCGGCAA TGATCCAGGT ATTGTCCCGA 
GAGGAGATGG ATGGGGGCCT CCGTAAAGCT ATGAGAGTCG TCAGCACTTG GTCTGAGGAT 
GACATTCTCA AGCCGGGACA AGTTTTCATT GTCAAGTCCT TTCTTCCTGA GGTTGTGCGG 
ACATGGCATA AAATCTTCCA GGAGAGCACT GTGCTTCATC TTTGCCTCAG GGAAATTCAA 
CAACAAAGAG CTGCTCAAAA ATTGATCTAT ACCTTCAACC AAGTGAAACC ACAAACCATA 
CCCTACACAC CAAGGTTCCT GGAAGTTTTC TTAATCTACT GCCATTCAGC CAACCAGTGG 
TTGACCATTG AGAAGTATAT GACAGGGGAG TTCCGGAAGT ATAACAACAA CAATGGTGAT 
GAAATCACCC CCACCAACAC CCTGGAGGAG CTGATGTTGG CTTTCTCTCA CTGGACCTAT 
GAGTACACTC GGGGAGAGCT GCTGGTTTTA GATTTGCAAG GTGTTGGAGA AAATTTGACA 
GATCCATCTG TTATAAAACC TGAAGTCAAA CAATCAAGAG GAATGGTGTT TGGACCGGCC 
AATTTGGGGG AAGATGCAAT TAGAAACTTC ATTGCAAAAC ATCATTGTAA CTCCTGCTGC 
CGGAAGCTCA AACTCCCGGA TTTAAAAAGA AATGACTATT CCCCTGAAAG GATAAATTCC 
ACCTTTGGAC TTGAGATAAA AATAGAATCA GCTGAGGAGC CTCCAGCAAG GGAGACGGGT 
AGAAATTCCC CAGAAGATGA TATGCAACTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516552.1
CDS	239..6031
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518250.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518250.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GATCTGTATT 
TCAGAACCTG GGAAGTTTAC CCAAAAGGTG AAGGTATGGA TTAGTGAGTA CTGGAACTTA 
ACAGAAACTG TGGCCATTGG CCTGTTTTCA GCTGGCTTCG TCCTTCGATG GGGTGACCCT 
CCTTTTCACA CAGCGGGAAG ACTGATCTAC TGCATAGACA TCATATTCTG GTTCTCACGG 
CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCAT ATGTGACCAT GATTGCAAAA 
ATGACAGCAA ACATGTTCTA TATTGTGATC ATCATGGCCA TAGTCCTGCT GAGCTTTGGA 
GTGGCACGCA AGGCCATCCT TTCGCCAAAA GAGCCACCAT CTTGGAGTCT AGCTCGAGAT 
ATTGTATTTG AGCCATACTG GATGATATAC GGAGAAGTCT ATGCTGGAGA AATAGATGTT 
TGTTCAAGCC AGCCATCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCTT GCAAGCTGTC 
TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT CAACAACGTT 
TACTTAGATA TGGAATCCAT TTCAAATAAC CTGTGGAAAT ACAACCGCTA TCGCTACATC 
ATGACCTACC ACGAGAAGCC CTGGCTGCCC CCACCTCTCA TCCTGCTGAG CCACGTGGGC 
CTTCTCCTCC GCCGCCTGTG CTGTCATCGA GCTCCTCACG ACCAAGAAGA GGGTGACGTT 
GGATTAAAAC TCTACCTCAG TAAGGAGGAT CTGAAAAAAC TTCATGATTT TGAGGAGCAG 
TGCGTGGAAA AATACTTCCA TGAGAAGATG GAAGATGTGA ATTGTAGTTG TGAGGAACGA 
ATCCGAGTGA CATCAGAAAG GGTTACAGAG ATGTACTTCC AGCTGAAAGA AATGAATGAA 
AAGGTGTCTT TTATAAAGGA CTCCTTACTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG 
GATCTCTCTG CCCTGACTGT GGATACCCTG AAAGTCCTTT CTGCTGTTGA CACTTTGCAA 
GAGGATGAGG CTCTCCTGGC CAAGAGAAAG CATTCTACTT GCAAAAAACT TCCCCACAGC 
TGGAGCAATG TCATCTGTGC AGAGGTTCTA GGCAGCATGG AGATCGCTGG AGAGAAGAAA 
TACCAGTATT ATAGCATGCC CTCTTCTTTG CTGAGGAGCC TGGCTGGAGG CCGGCATCCC 
CCAAGAGTGC AGAGGGGGGC ACTTCTTGAG ATTACAAACA GTAAAAGAGA GGCTACAAAT 
GTAAGAAATG ACCAGGAAAG GCAAGAAACA CAAAGTAGTA TAGTGGTTTC TGGGGTGTCT 
CCTAACAGGC AAGCACACTC AAAGTATGGC CAGTTTCTTC TGGTCCCCTC TAATCTAAAG 
CGAGTTCCTT TTTCAGCAGA AACTGTCTTG CCTCTGTCCA GACCCTCTGT GCCAGATGTG 
CTGGCAACTG AACAGGACAT CCAGACTGAG GTTCTTGTTC ATCTGACTGG GCAGACCCCA 
GTTGTCTCTG ACTGGGCATC AGTGGATGAA CCCAAGGAAA AGCACGAGCC TATTGCTCAC 
TTACTGGATG GACAAGACAA GGCAGAGCAA GTGCTACCCA CTTTGAGTTG CACACCTGAA 
CCCATGACAA TGAGCTCCCC TCTTTCCCAA GCCAAGATCA TGCAAACTGG AGGTGGATAT 
GTAAACTGGG CATTTTCAGA AGGTGATGAA ACTGGTGTGT TTAGCATCAA GAAAAAGTGG 
CAAACCTGCT TGCCCTCCAC TTGTGACAGT GATTCCTCTC GGAGTGAACA GCACCAGAAG 
CAGGCCCAGG ACAGCTCCCT ATCTGATAAC TCAACAAGAT CGGCCCAGAG TAGTGAATGC 
TCAGAGGTGG GACCATGGCT TCAGCCAAAC ACATCCTTTT GGATCAATCC TCTCCGCAGA 
TACAGGCCCT TCGCTAGGAG TCATAGTTTT AGATTCCATA AGGAGGAGAA ATTGATGAAG 
ATCTGTAAGA TTAAAAATCT TTCAGGCTCT TCAGAAATAG GGCAGGGAGC ATGGGTCAAA 
GCGAAAATGC TAACCAAAGA CAGGAGACTG TCAAAGAAAA AGAAGAATAC TCAAGGACTC 
CAGGTGCCAA TCATAACAGT CAATGCCTGC TCTCAGAGTG ACCAGTTGAA TCCAGAGCCA 
GGAGAAAACA GCATCTCTGA AGAGGAGTAC AGCAAGAACT GGTTCACAGT GTCCAAATTT 
AGTCACACAG GTGTAGAACC TTACATACAT CAGAAAATGA AAACTAAAGA AATTGGACAA 
TGTGCTATAC AAATCAGTGA TTACCTAAAG CAGTCTCAAG AGGATCTCAG CAAAAACTCT 
TTGTGGAATT CCAGGAGCAC CAACCTCAAT AGGAACTCCC TGCTGAAAAG TTCAATTGGA 
GTTGACAAGA TCTCAGCCTC CTTAAAAAGC CCTCAAGAGC CTCACCATCA TTATTCAGCC 
ATTGAAAGGA ATAATTTAAT GAGGCTTTCT CAGACCATAC CATTTACACC AGTCCAACTG 
TTTGCAGGAG AAGAAATAAC TGTCTACAGG TTGGAGGAGA GTTCCCCTTT AAACCTTGAT 
AAAAGCATGT CCTCTTGGTC TCAGCGTGGG AGAGCGGCAA TGATCCAGGT ATTGTCCCGA 
GAGGAGATGG ATGGGGGCCT CCGTAAAGCT ATGAGAGTCG TCAGCACTTG GTCTGAGGAT 
GACATTCTCA AGCCGGGACA AGTTTTCATT GTCAAGTCCT TTCTTCCTGA GGTTGTGCGG 
ACATGGCATA AAATCTTCCA GGAGAGCACT GTGCTTCATC TTTGCCTCAG GGAAATTCAA 
CAACAAAGAG CTGCTCAAAA ATTGATCTAT ACCTTCAACC AAGTGAAACC ACAAACCATA 
CCCTACACAC CAAGGTTCCT GGAAGTTTTC TTAATCTACT GCCATTCAGC CAACCAGTGG 
TTGACCATTG AGAAGTATAT GACAGGGGAG TTCCGGAAGT ATAACAACAA CAATGGTGAT 
GAAATCACCC CCACCAACAC CCTGGAGGAG CTGATGTTGG CTTTCTCTCA CTGGACCTAT 
GAGTACACTC GGGGAGAGCT GCTGGTTTTA GATTTGCAAG GTGTTGGAGA AAATTTGACA 
GATCCATCTG TTATAAAACC TGAAGTCAAA CAATCAAGAG GAATGGTGTT TGGACCGGCC 
AATTTGGGGG AAGATGCAAT TAGAAACTTC ATTGCAAAAC ATCATTGTAA CTCCTGCTGC 
CGGAAGCTCA AACTCCCGGA TTTAAAAAGA AATGACTATT CCCCTGAAAG GATAAATTCC 
ACCTTTGGAC TTGAGATAAA AATAGAATCA GCTGAGGAGC CTCCAGCAAG GGAGACGGGT 
AGAAATTCCC CAGAAGATGA TATGCAACTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518252.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516554.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X12, mRNA.

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CloneID	OHu75947
Clone ID Related Accession (Same CDS sequence)	XM_011518252.2
Accession Version	XM_011518252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGAC CTTAAATGCT GAAGATATTA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516554.1
CDS	239..5323
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLTLNAEDII

Target ORF information:

RefSeq Version	XM_011518252.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518252.2

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGAC CTTAAATGCT GAAGATATTA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( NM_001177311.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001170782.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant c, mRNA.

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CloneID	OHu22680
Clone ID Related Accession (Same CDS sequence)	NM_001177311.1
Accession Version	NM_001177311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY333284.1, AF350881.2 and AL354795.17. Transcript Variant: This variant (c) contains an alternate 5' terminal exon compared to variant a. This results in a shorter isoform (c) with a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY333284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGCGC CGGTTACGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC 
TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT 
TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG 
CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC 
ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT 
TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC 
ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA 
GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG 
CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT 
GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG 
TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC 
TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC 
AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC 
GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG 
ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC 
CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG 
GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT 
TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC 
TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT 
TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG 
AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA 
GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC 
CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT 
ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC 
TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT 
CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC 
AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC 
CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA 
TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT 
TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG 
TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC 
CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG 
AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG 
CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG 
ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC 
CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA 
AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA 
GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC 
CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC 
CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT 
CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT 
ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG 
CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG 
GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG 
ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC 
GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC 
ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA 
TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC 
ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA 
TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC 
TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT 
ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG 
ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA 
TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC 
ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC 
GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA 
CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG 
AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC 
CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC 
AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT 
TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT 
TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG 
GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC 
CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC 
AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT 
ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT 
CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC 
AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT 
CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA 
AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC 
ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC 
ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG 
TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT 
CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA 
TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT 
TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT 
AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA 
GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA 
AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT 
GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC 
TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG 
AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA 
GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC 
CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG 
CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA 
CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG 
AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA 
ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC 
GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC 
TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT 
CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC 
CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC 
TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG 
TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG 
GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA 
GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA 
GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA 
CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT 
TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG 
CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001170782.1
CDS	100..6153
Translation	MTAPVTSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	NM_001177311.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177311.1

ORF Insert Sequence:

ATGACAGCGC CGGTTACGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC 
TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT 
TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG 
CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC 
ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT 
TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC 
ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA 
GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG 
CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT 
GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG 
TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC 
TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC 
AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC 
GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG 
ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC 
CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG 
GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT 
TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC 
TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT 
TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG 
AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA 
GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC 
CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT 
ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC 
TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT 
CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC 
AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC 
CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA 
TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT 
TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG 
TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC 
CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG 
AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG 
CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG 
ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC 
CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA 
AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA 
GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC 
CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC 
CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT 
CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT 
ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG 
CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG 
GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG 
ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC 
GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC 
ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA 
TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC 
ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA 
TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC 
TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT 
ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG 
ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA 
TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC 
ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC 
GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA 
CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG 
AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC 
CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC 
AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT 
TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT 
TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG 
GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC 
CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC 
AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT 
ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT 
CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC 
AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT 
CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA 
AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC 
ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC 
ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG 
TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT 
CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA 
TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT 
TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT 
AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA 
GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA 
AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT 
GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC 
TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG 
AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA 
GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC 
CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG 
CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA 
CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG 
AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA 
ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC 
GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC 
TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT 
CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC 
CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC 
TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG 
TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG 
GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA 
GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA 
GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA 
CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT 
TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG 
CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( NM_001177310.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001170781.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant b, mRNA.

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CloneID	OHu22981
Clone ID Related Accession (Same CDS sequence)	NM_001177310.1
Accession Version	NM_001177310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY333283.1, AF350881.2 and AL354795.17. Transcript Variant: This variant (b) contains an alternate 5' terminal exon compared to variant a. This results in a shorter isoform (b) with a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY333283.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTATCC TATCTAAGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC 
TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT 
TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG 
CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC 
ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT 
TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC 
ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA 
GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG 
CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT 
GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG 
TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC 
TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC 
AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC 
GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG 
ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC 
CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG 
GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT 
TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC 
TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT 
TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG 
AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA 
GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC 
CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT 
ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC 
TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT 
CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC 
AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC 
CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA 
TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT 
TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG 
TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC 
CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG 
AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG 
CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG 
ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC 
CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA 
AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA 
GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC 
CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC 
CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT 
CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT 
ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG 
CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG 
GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG 
ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC 
GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC 
ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA 
TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC 
ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA 
TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC 
TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT 
ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG 
ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA 
TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC 
ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC 
GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA 
CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG 
AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC 
CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC 
AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT 
TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT 
TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG 
GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC 
CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC 
AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT 
ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT 
CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC 
AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT 
CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA 
AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC 
ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC 
ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG 
TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT 
CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA 
TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT 
TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT 
AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA 
GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA 
AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT 
GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC 
TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG 
AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA 
GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC 
CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG 
CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA 
CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG 
AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA 
ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC 
GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC 
TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT 
CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC 
CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC 
TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG 
TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG 
GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA 
GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA 
GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA 
CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT 
TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG 
CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001170781.1
CDS	73..6126
Translation	MIILSKSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	NM_001177310.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177310.1

ORF Insert Sequence:

ATGATTATCC TATCTAAGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC 
TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT 
TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG 
CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC 
ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT 
TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC 
ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA 
GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG 
CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT 
GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG 
TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC 
TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC 
AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC 
GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG 
ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC 
CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG 
GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT 
TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC 
TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT 
TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG 
AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA 
GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC 
CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT 
ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC 
TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT 
CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC 
AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC 
CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA 
TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT 
TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG 
TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC 
CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG 
AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG 
CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG 
ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC 
CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA 
AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA 
GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC 
CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC 
CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT 
CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT 
ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG 
CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG 
GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG 
ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC 
GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC 
ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA 
TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC 
ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA 
TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC 
TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT 
ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG 
ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA 
TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC 
ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC 
GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA 
CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG 
AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC 
CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC 
AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT 
TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT 
TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG 
GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC 
CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC 
AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT 
ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT 
CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC 
AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT 
CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA 
AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC 
ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC 
ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG 
TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT 
CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA 
TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT 
TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT 
AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA 
GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA 
AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT 
GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC 
TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG 
AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA 
GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC 
CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG 
CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA 
CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG 
AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA 
ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC 
GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC 
TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT 
CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC 
CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC 
TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG 
TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG 
GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA 
GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA 
GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA 
CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT 
TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG 
CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM6 ( XM_011518244.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516546.1 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA.

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CloneID	OHu75939
Clone ID Related Accession (Same CDS sequence)	XM_011518244.1
Accession Version	XM_011518244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG GAGAAGAAAT AACTGTCTAC 
AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT 
GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA 
GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC 
ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC 
ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC 
TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT 
TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG 
GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG 
GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT 
TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC 
AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC 
TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA 
AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA 
TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516546.1
CDS	239..6304
Translation	MKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL

Target ORF information:

RefSeq Version	XM_011518244.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518244.1

ORF Insert Sequence:

ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT 
CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC 
CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA 
TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC 
CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG 
GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC 
TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC 
CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT 
ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA 
AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT 
GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA 
CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA 
AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC 
CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC 
CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT 
AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA 
CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA 
CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC 
ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG 
CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG 
GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG 
GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA 
GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT 
ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT 
ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC 
ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC 
CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA 
AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT 
ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG 
TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG 
CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT 
GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT 
GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG 
GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG 
AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT 
TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG 
AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG 
GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG 
TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT 
GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT 
GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC 
AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG 
TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC 
ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA 
AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG 
TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG 
ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT 
CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT 
GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG 
CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG 
ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT 
CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC 
ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA 
AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG 
CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT 
CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG 
GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG 
AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT 
ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC 
TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT 
ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG 
AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG 
GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT 
TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT 
CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA 
GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG 
TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT 
GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG 
ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG 
GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA 
GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT 
TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT 
GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT 
GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT 
GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG 
CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT 
AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA 
GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG 
AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT 
GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG 
GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC 
ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC 
CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC 
CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA 
AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG 
CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG GAGAAGAAAT AACTGTCTAC 
AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT 
GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA 
GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC 
ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC 
ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC 
TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT 
TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG 
GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG 
GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT 
TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC 
AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC 
TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA 
AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA 
TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRPM6
Entrez Gene ID	140803
Full Name	transient receptor potential cation channel subfamily M member 6
Synonyms	CHAK2,HMGX,HOMG,HOMG1,HSH
General protein information	Preferred Names transient receptor potential cation channel subfamily M member 6 Names transient receptor potential cation channel subfamily M member 6 channel kinase 2 melastatin-related TRP cation channel 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q21.13
Summary	This gene is predominantly expressed in the kidney and colon, and encodes a protein containing an ion channel domain and a protein kinase domain. It is crucial for magnesium homeostasis, and plays an essential role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Apr 2010].
Disorder MIM:	607009
Disorder Html:	Hypomagnesemia 1, intestinal, 602014 (3)

Bos taurus (cattle)	TRPM6	XP_005210098.1
Rattus norvegicus (Norway rat)	Trpm6	XP_006231205.1
Canis lupus familiaris (dog)	TRPM6	XP_005615911.1
Gallus gallus (chicken)	TRPM6	XP_003643069.2
Xenopus tropicalis (tropical clawed frog)	trpm6	XP_002940079.2
Homo sapiens (human)	TRPM6	NP_001170781.1
Mus musculus (house mouse)	Trpm6	NP_700466.1
Pan troglodytes (chimpanzee)	TRPM6	XP_003312159.1
	TRPM6	XP_002800188.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRPM6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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