TRPM6 cDNA ORF clone, Homo sapiens(human)

The following TRPM6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23065 NM_017662.5
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$779.50-$1091.30
$1559.00
OHu75950 XM_011518255.2
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu75946 XM_011518251.2
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22 $951.30
$1359.00
OHu98449 XM_017014287.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu98450 XM_017014288.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu98451 XM_017014289.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu75940 XM_011518245.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu75941 XM_011518246.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu75942 XM_011518247.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu75943 XM_011518248.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu75944 XM_011518249.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu75945 XM_011518250.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu75947 XM_011518252.2
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu22680 NM_001177311.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant c, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu22981 NM_001177310.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant b, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu75939 XM_011518244.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OHu23065 NM_017662.4 Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$779.50-$1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23065
    Clone ID Related Accession (Same CDS sequence) NM_017662.5 , NM_017662.4
    Accession Version NM_017662.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF350881.2 and AL354795.17. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_017662.4. Transcript Variant: This variant (a) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF350881.2, AY333282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000360774.6/ ENSP00000354006.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC
    TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG
    CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT
    AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT
    TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG
    AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG
    ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA
    GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA
    GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG
    GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG
    GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA
    GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA
    AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA
    AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA
    GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG
    CAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060132.3
    CDS66..6134
    Translation

    Target ORF information:

    RefSeq Version NM_017662.5
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017662.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC
    TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG
    CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT
    AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT
    TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG
    AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG
    ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA
    GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA
    GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG
    GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG
    GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA
    GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA
    AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA
    AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA
    GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG
    CAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23065
    Clone ID Related Accession (Same CDS sequence) NM_017662.5 , NM_017662.4
    Accession Version NM_017662.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-15
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF350881.2 and AL354795.17. This sequence is a reference standard in the RefSeqGene project. On Apr 17, 2008 this sequence version replaced NM_017662.3. Transcript Variant: This variant (a) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF350881.2, AY333282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC
    TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG
    CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT
    AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT
    TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG
    AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG
    ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA
    GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA
    GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG
    GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG
    GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA
    GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA
    AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA
    AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA
    GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG
    CAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060132.3
    CDS239..6307
    Translation

    Target ORF information:

    RefSeq Version NM_017662.4
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017662.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC
    TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG
    CGTGGGAGAG CGGCAATGAT CCAGGTATTG TCCCGAGAGG AGATGGATGG GGGCCTCCGT
    AAAGCTATGA GAGTCGTCAG CACTTGGTCT GAGGATGACA TTCTCAAGCC GGGACAAGTT
    TTCATTGTCA AGTCCTTTCT TCCTGAGGTT GTGCGGACAT GGCATAAAAT CTTCCAGGAG
    AGCACTGTGC TTCATCTTTG CCTCAGGGAA ATTCAACAAC AAAGAGCTGC TCAAAAATTG
    ATCTATACCT TCAACCAAGT GAAACCACAA ACCATACCCT ACACACCAAG GTTCCTGGAA
    GTTTTCTTAA TCTACTGCCA TTCAGCCAAC CAGTGGTTGA CCATTGAGAA GTATATGACA
    GGGGAGTTCC GGAAGTATAA CAACAACAAT GGTGATGAAA TCACCCCCAC CAACACCCTG
    GAGGAGCTGA TGTTGGCTTT CTCTCACTGG ACCTATGAGT ACACTCGGGG AGAGCTGCTG
    GTTTTAGATT TGCAAGGTGT TGGAGAAAAT TTGACAGATC CATCTGTTAT AAAACCTGAA
    GTCAAACAAT CAAGAGGAAT GGTGTTTGGA CCGGCCAATT TGGGGGAAGA TGCAATTAGA
    AACTTCATTG CAAAACATCA TTGTAACTCC TGCTGCCGGA AGCTCAAACT CCCGGATTTA
    AAAAGAAATG ACTATTCCCC TGAAAGGATA AATTCCACCT TTGGACTTGA GATAAAAATA
    GAATCAGCTG AGGAGCCTCC AGCAAGGGAG ACGGGTAGAA ATTCCCCAGA AGATGATATG
    CAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75950
    Clone ID Related Accession (Same CDS sequence) XM_011518255.2
    Accession Version XM_011518255.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGATC
    TCCTACATGC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516557.1
    CDS239..3793
    Translation

    Target ORF information:

    RefSeq Version XM_011518255.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518255.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGATC
    TCCTACATGC ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75946
    Clone ID Related Accession (Same CDS sequence) XM_011518251.2
    Accession Version XM_011518251.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCACTCGC ACTTCATCCT GTCTGATGAT GGGACCGTGG GCAAGTATGG AAATGAAATG 
    AAGCTCAGAA GGAACCTGGA GAAGTACCTC TCTCTGCAGA AAATACACTG CCGCTCAAGA
    CAAGGCGTGC CGGTCGTGGG GCTGGTGGTG GAAGGCGGTC CCAACGTCAT CCTGTCAGTG
    TGGGAGACTG TCAAGGACAA GGACCCAGTG GTGGTGTGTG AGGGCACAGG TAGGGCGGCT
    GACCTCCTGG CCTTCACACA CAAACACCTG GCAGATGAAG GGATGCTGCG ACCTCAGGTG
    AAAGAGGAGA TCATCTGCAT GATTCAGAAC ACTTTCAACT TTAGTCTTAA ACAGTCCAAG
    CACCTTTTCC AAATTCTAAT GGAGTGTATG GTTCACAGGG ATTGTATTAC CATATTTGAT
    GCTGACTCTG AAGAGCAGCA AGACCTGGAC TTAGCAATCC TAACAGCTTT GCTGAAGGGC
    ACAAATTTAT CAGCGTCAGA GCAATTAAAT CTGGCAATGG CTTGGGACAG GGTGGACATT
    GCCAAGAAAC ATATCCTAAT TTATGAACAA CACTGGAAGC CTGATGCCCT GGAACAAGCA
    ATGTCAGATG CTTTAGTGAT GGATCGGGTG GATTTTGTGA AGCTCTTAAT AGAATATGGA
    GTGAACCTCC ATCGCTTTCT TACCATCCCT CGACTGGAAG AGCTCTACAA TACAAAACAA
    GGACCTACTA ATACACTCTT GCATCATCTC GTCCAAGATG TGAAACAGCA TACCCTTCTT
    TCAGGCTACC GAATAACCTT GATTGACATT GGATTAGTAG TAGAATACCT CATTGGTAGA
    GCATATCGCA GCAACTACAC TAGAAAACAT TTCAGAGCCC TCTACAACAA CCTCTACAGA
    AAATACAAGC ACCAGAGACA CTCCTCAGGA AATAGAAATG AGTCTGCAGA AAGTACGCTG
    CACTCCCAGT TCATTAGAAC TGCACAGCCA TACAAATTCA AGGAAAAGTC TATAGTCCTT
    CATAAATCAA GGAAGAAGTC AAAAGAACAA AATGTATCAG ATGACCCTGA GTCTACTGGC
    TTTCTTTACC CTTACAATGA CCTGCTGGTT TGGGCTGTGC TGATGAAAAG GCAGAAGATG
    GCTATGTTCT TCTGGCAGCA TGGAGAGGAG GCCACGGTTA AAGCCGTGAT TGCGTGTATC
    CTCTACCGGG CAATGGCCCA TGAAGCTAAG GAGAGTCACA TGGTGGATGA TGCCTCAGAA
    GAGTTGAAGA ATTACTCAAA ACAGTTTGGC CAGCTGGCTC TGGACTTGTT GGAGAAGGCA
    TTCAAGCAGA ATGAGCGCAT GGCCATGACG CTGTTGACGT ATGAACTCAG GAACTGGAGC
    AATTCGACCT GCCTTAAACT GGCCGTGTCG GGAGGATTAC GACCCTTTGT TTCACATACT
    TGTACCCAGA TGCTACTGAC AGACATGTGG ATGGGGAGGC TGAAAATGAG GAAAAACTCT
    TGGTTAAAGA TTATTATAAG CATTATTTTA CCACCCACCA TTTTGACACT GGAATTTAAA
    AGCAAAGCTG AGATGTCACA TGTTCCCCAG TCCCAGGACT TCCAATTTAT GTGGTATTAC
    AGTGACCAGA ACGCCAGCAG TTCCAAAGAA AGTGCTTCTG TGAAAGAGTA TGATTTGGAA
    AGGGGCCATG ATGAGAAACT GGATGAAAAT CAGCATTTTG GTTTGGAAAG TGGGCACCAA
    CACCTTCCGT GGACCAGGAA AGTCTATGAG TTCTACAGTG CTCCAATTGT CAAGTTTTGG
    TTTTATACGA TGGCGTATTT GGCATTCCTC ATGCTGTTCA CTTACACCGT GTTGGTGGAG
    ATGCAGCCCC AGCCCAGCGT GCAGGAGTGG CTTGTTAGCA TTTACATCTT CACCAATGCT
    ATTGAGGTGG TCAGGGAGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG
    GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT
    GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC
    ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA
    GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC
    ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG
    CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA
    GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT
    TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC
    CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG
    TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA
    CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT
    CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG
    AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA
    GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG
    TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT
    TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA
    GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT
    TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC
    AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG
    AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT
    ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA
    AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG
    TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT
    CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT
    CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC
    AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG
    CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC
    AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT
    GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT
    TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA
    ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA
    TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA
    TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA
    GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA
    AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT
    CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC
    AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG
    AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG
    TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG
    AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT
    CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG
    ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG
    GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA
    GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG
    AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC
    AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG
    CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC
    TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA
    ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC
    CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG
    ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT
    TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA
    TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT
    GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT
    GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT
    GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516553.1
    CDS219..5558
    Translation

    Target ORF information:

    RefSeq Version XM_011518251.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518251.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCACTCGC ACTTCATCCT GTCTGATGAT GGGACCGTGG GCAAGTATGG AAATGAAATG 
    AAGCTCAGAA GGAACCTGGA GAAGTACCTC TCTCTGCAGA AAATACACTG CCGCTCAAGA
    CAAGGCGTGC CGGTCGTGGG GCTGGTGGTG GAAGGCGGTC CCAACGTCAT CCTGTCAGTG
    TGGGAGACTG TCAAGGACAA GGACCCAGTG GTGGTGTGTG AGGGCACAGG TAGGGCGGCT
    GACCTCCTGG CCTTCACACA CAAACACCTG GCAGATGAAG GGATGCTGCG ACCTCAGGTG
    AAAGAGGAGA TCATCTGCAT GATTCAGAAC ACTTTCAACT TTAGTCTTAA ACAGTCCAAG
    CACCTTTTCC AAATTCTAAT GGAGTGTATG GTTCACAGGG ATTGTATTAC CATATTTGAT
    GCTGACTCTG AAGAGCAGCA AGACCTGGAC TTAGCAATCC TAACAGCTTT GCTGAAGGGC
    ACAAATTTAT CAGCGTCAGA GCAATTAAAT CTGGCAATGG CTTGGGACAG GGTGGACATT
    GCCAAGAAAC ATATCCTAAT TTATGAACAA CACTGGAAGC CTGATGCCCT GGAACAAGCA
    ATGTCAGATG CTTTAGTGAT GGATCGGGTG GATTTTGTGA AGCTCTTAAT AGAATATGGA
    GTGAACCTCC ATCGCTTTCT TACCATCCCT CGACTGGAAG AGCTCTACAA TACAAAACAA
    GGACCTACTA ATACACTCTT GCATCATCTC GTCCAAGATG TGAAACAGCA TACCCTTCTT
    TCAGGCTACC GAATAACCTT GATTGACATT GGATTAGTAG TAGAATACCT CATTGGTAGA
    GCATATCGCA GCAACTACAC TAGAAAACAT TTCAGAGCCC TCTACAACAA CCTCTACAGA
    AAATACAAGC ACCAGAGACA CTCCTCAGGA AATAGAAATG AGTCTGCAGA AAGTACGCTG
    CACTCCCAGT TCATTAGAAC TGCACAGCCA TACAAATTCA AGGAAAAGTC TATAGTCCTT
    CATAAATCAA GGAAGAAGTC AAAAGAACAA AATGTATCAG ATGACCCTGA GTCTACTGGC
    TTTCTTTACC CTTACAATGA CCTGCTGGTT TGGGCTGTGC TGATGAAAAG GCAGAAGATG
    GCTATGTTCT TCTGGCAGCA TGGAGAGGAG GCCACGGTTA AAGCCGTGAT TGCGTGTATC
    CTCTACCGGG CAATGGCCCA TGAAGCTAAG GAGAGTCACA TGGTGGATGA TGCCTCAGAA
    GAGTTGAAGA ATTACTCAAA ACAGTTTGGC CAGCTGGCTC TGGACTTGTT GGAGAAGGCA
    TTCAAGCAGA ATGAGCGCAT GGCCATGACG CTGTTGACGT ATGAACTCAG GAACTGGAGC
    AATTCGACCT GCCTTAAACT GGCCGTGTCG GGAGGATTAC GACCCTTTGT TTCACATACT
    TGTACCCAGA TGCTACTGAC AGACATGTGG ATGGGGAGGC TGAAAATGAG GAAAAACTCT
    TGGTTAAAGA TTATTATAAG CATTATTTTA CCACCCACCA TTTTGACACT GGAATTTAAA
    AGCAAAGCTG AGATGTCACA TGTTCCCCAG TCCCAGGACT TCCAATTTAT GTGGTATTAC
    AGTGACCAGA ACGCCAGCAG TTCCAAAGAA AGTGCTTCTG TGAAAGAGTA TGATTTGGAA
    AGGGGCCATG ATGAGAAACT GGATGAAAAT CAGCATTTTG GTTTGGAAAG TGGGCACCAA
    CACCTTCCGT GGACCAGGAA AGTCTATGAG TTCTACAGTG CTCCAATTGT CAAGTTTTGG
    TTTTATACGA TGGCGTATTT GGCATTCCTC ATGCTGTTCA CTTACACCGT GTTGGTGGAG
    ATGCAGCCCC AGCCCAGCGT GCAGGAGTGG CTTGTTAGCA TTTACATCTT CACCAATGCT
    ATTGAGGTGG TCAGGGAGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG
    GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT
    GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC
    ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA
    GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC
    ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG
    CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA
    GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT
    TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC
    CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG
    TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA
    CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT
    CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG
    AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA
    GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG
    TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT
    TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA
    GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT
    TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC
    AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG
    AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT
    ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA
    AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG
    TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT
    CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT
    CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC
    AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG
    CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC
    AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT
    GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT
    TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA
    ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA
    TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA
    TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA
    GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA
    AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT
    CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC
    AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG
    AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG
    TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG
    AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT
    CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG
    ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG
    GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA
    GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG
    AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC
    AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG
    CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC
    TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA
    ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC
    CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG
    ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT
    TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA
    TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT
    GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT
    GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT
    GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98449
    Clone ID Related Accession (Same CDS sequence) XM_017014287.1
    Accession Version XM_017014287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA
    AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC
    CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG
    AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC
    GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG
    GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC
    TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA
    CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC
    TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA
    ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG
    ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA
    TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA
    GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG
    GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA
    ATTGTCAAGT TTTGGTTTTA TACGATCTGT ATTTCAGAAC CTGGGAAGTT TACCCAAAAG
    GTGAAGGTAT GGATTAGTGA GTACTGGAAC TTAACAGAAA CTGTGGCCAT TGGCCTGTTT
    TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC CCTCCTTTTC ACACAGCGGG AAGACTGATC
    TACTGCATAG ACATCATATT CTGGTTCTCA CGGCTCCTGG ACTTCTTTGC TGTGAATCAA
    CATGCAGGTC CATATGTGAC CATGATTGCA AAAATGACAG CAAACATGTT CTATATTGTG
    ATCATCATGG CCATAGTCCT GCTGAGCTTT GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA
    AAAGAGCCAC CATCTTGGAG TCTAGCTCGA GATATTGTAT TTGAGCCATA CTGGATGATA
    TACGGAGAAG TCTATGCTGG AGAAATAGAT GTTTGTTCAA GCCAGCCATC CTGCCCTCCT
    GGTTCTTTTC TTACTCCATT CTTGCAAGCT GTCTACCTCT TCGTGCAATA TATCATCATG
    GTGAACCTGT TGATTGCTTT CTTCAACAAC GTTTACTTAG ATATGGAATC CATTTCAAAT
    AACCTGTGGA AATACAACCG CTATCGCTAC ATCATGACCT ACCACGAGAA GCCCTGGCTG
    CCCCCACCTC TCATCCTGCT GAGCCACGTG GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT
    CGAGCTCCTC ACGACCAAGA AGAGGGTGAC GTTGGATTAA AACTCTACCT CAGTAAGGAG
    GATCTGAAAA AACTTCATGA TTTTGAGGAG CAGTGCGTGG AAAAATACTT CCATGAGAAG
    ATGGAAGATG TGAATTGTAG TTGTGAGGAA CGAATCCGAG TGACATCAGA AAGGGTTACA
    GAGATGTACT TCCAGCTGAA AGAAATGAAT GAAAAGGTGT CTTTTATAAA GGACTCCTTA
    CTGTCTTTGG ACAGCCAGGT GGGACACCTG CAGGATCTCT CTGCCCTGAC TGTGGATACC
    CTGAAAGTCC TTTCTGCTGT TGACACTTTG CAAGAGGATG AGGCTCTCCT GGCCAAGAGA
    AAGCATTCTA CTTGCAAAAA ACTTCCCCAC AGCTGGAGCA ATGTCATCTG TGCAGAGGTT
    CTAGGCAGCA TGGAGATCGC TGGAGAGAAG AAATACCAGT ATTATAGCAT GCCCTCTTCT
    TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT CCCCCAAGAG TGCAGAGGGG GGCACTTCTT
    GAGATTACAA ACAGTAAAAG AGAGGCTACA AATGTAAGAA ATGACCAGGA AAGGCAAGAA
    ACACAAAGTA GTATAGTGGT TTCTGGGGTG TCTCCTAACA GGCAAGCACA CTCAAAGTAT
    GGCCAGTTTC TTCTGGTCCC CTCTAATCTA AAGCGAGTTC CTTTTTCAGC AGAAACTGTC
    TTGCCTCTGT CCAGACCCTC TGTGCCAGAT GTGCTGGCAA CTGAACAGGA CATCCAGACT
    GAGGTTCTTG TTCATCTGAC TGGGCAGACC CCAGTTGTCT CTGACTGGGC ATCAGTGGAT
    GAACCCAAGG AAAAGCACGA GCCTATTGCT CACTTACTGG ATGGACAAGA CAAGGCAGAG
    CAAGTGCTAC CCACTTTGAG TTGCACACCT GAACCCATGA CAATGAGCTC CCCTCTTTCC
    CAAGCCAAGA TCATGCAAAC TGGAGGTGGA TATGTAAACT GGGCATTTTC AGAAGGTGAT
    GAAACTGGTG TGTTTAGCAT CAAGAAAAAG TGGCAAACCT GCTTGCCCTC CACTTGTGAC
    AGTGATTCCT CTCGGAGTGA ACAGCACCAG AAGCAGGCCC AGGACAGCTC CCTATCTGAT
    AACTCAACAA GATCGGCCCA GAGTAGTGAA TGCTCAGAGG TGGGACCATG GCTTCAGCCA
    AACACATCCT TTTGGATCAA TCCTCTCCGC AGATACAGGC CCTTCGCTAG GAGTCATAGT
    TTTAGATTCC ATAAGGAGGA GAAATTGATG AAGATCTGTA AGATTAAAAA TCTTTCAGGC
    TCTTCAGAAA TAGGGCAGGG AGCATGGGTC AAAGCGAAAA TGCTAACCAA AGACAGGAGA
    CTGTCAAAGA AAAAGAAGAA TACTCAAGGA CTCCAGGTGC CAATCATAAC AGTCAATGCC
    TGCTCTCAGA GTGACCAGTT GAATCCAGAG CCAGGAGAAA ACAGCATCTC TGAAGAGGAG
    TACAGCAAGA ACTGGTTCAC AGTGTCCAAA TTTAGTCACA CAGGTGTAGA ACCTTACATA
    CATCAGAAAA TGAAAACTAA AGAAATTGGA CAATGTGCTA TACAAATCAG TGATTACCTA
    AAGCAGTCTC AAGAGGATCT CAGCAAAAAC TCTTTGTGGA ATTCCAGGAG CACCAACCTC
    AATAGGAACT CCCTGCTGAA AAGTTCAATT GGAGTTGACA AGATCTCAGC CTCCTTAAAA
    AGCCCTCAAG AGCCTCACCA TCATTATTCA GCCATTGAAA GGAATAATTT AATGAGGCTT
    TCTCAGACCA TACCATTTAC ACCAGTCCAA CTGTTTGCAG GAGAAGAAAT AACTGTCTAC
    AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT
    GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA
    GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC
    ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC
    ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC
    TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT
    TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG
    GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG
    GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT
    TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC
    AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC
    TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA
    AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA
    TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA
    CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869776.1
    CDS239..5944
    Translation

    Target ORF information:

    RefSeq Version XM_017014287.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA
    AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC
    CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG
    AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC
    GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG
    GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC
    TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA
    CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC
    TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA
    ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG
    ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA
    TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA
    GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG
    GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA
    ATTGTCAAGT TTTGGTTTTA TACGATCTGT ATTTCAGAAC CTGGGAAGTT TACCCAAAAG
    GTGAAGGTAT GGATTAGTGA GTACTGGAAC TTAACAGAAA CTGTGGCCAT TGGCCTGTTT
    TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC CCTCCTTTTC ACACAGCGGG AAGACTGATC
    TACTGCATAG ACATCATATT CTGGTTCTCA CGGCTCCTGG ACTTCTTTGC TGTGAATCAA
    CATGCAGGTC CATATGTGAC CATGATTGCA AAAATGACAG CAAACATGTT CTATATTGTG
    ATCATCATGG CCATAGTCCT GCTGAGCTTT GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA
    AAAGAGCCAC CATCTTGGAG TCTAGCTCGA GATATTGTAT TTGAGCCATA CTGGATGATA
    TACGGAGAAG TCTATGCTGG AGAAATAGAT GTTTGTTCAA GCCAGCCATC CTGCCCTCCT
    GGTTCTTTTC TTACTCCATT CTTGCAAGCT GTCTACCTCT TCGTGCAATA TATCATCATG
    GTGAACCTGT TGATTGCTTT CTTCAACAAC GTTTACTTAG ATATGGAATC CATTTCAAAT
    AACCTGTGGA AATACAACCG CTATCGCTAC ATCATGACCT ACCACGAGAA GCCCTGGCTG
    CCCCCACCTC TCATCCTGCT GAGCCACGTG GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT
    CGAGCTCCTC ACGACCAAGA AGAGGGTGAC GTTGGATTAA AACTCTACCT CAGTAAGGAG
    GATCTGAAAA AACTTCATGA TTTTGAGGAG CAGTGCGTGG AAAAATACTT CCATGAGAAG
    ATGGAAGATG TGAATTGTAG TTGTGAGGAA CGAATCCGAG TGACATCAGA AAGGGTTACA
    GAGATGTACT TCCAGCTGAA AGAAATGAAT GAAAAGGTGT CTTTTATAAA GGACTCCTTA
    CTGTCTTTGG ACAGCCAGGT GGGACACCTG CAGGATCTCT CTGCCCTGAC TGTGGATACC
    CTGAAAGTCC TTTCTGCTGT TGACACTTTG CAAGAGGATG AGGCTCTCCT GGCCAAGAGA
    AAGCATTCTA CTTGCAAAAA ACTTCCCCAC AGCTGGAGCA ATGTCATCTG TGCAGAGGTT
    CTAGGCAGCA TGGAGATCGC TGGAGAGAAG AAATACCAGT ATTATAGCAT GCCCTCTTCT
    TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT CCCCCAAGAG TGCAGAGGGG GGCACTTCTT
    GAGATTACAA ACAGTAAAAG AGAGGCTACA AATGTAAGAA ATGACCAGGA AAGGCAAGAA
    ACACAAAGTA GTATAGTGGT TTCTGGGGTG TCTCCTAACA GGCAAGCACA CTCAAAGTAT
    GGCCAGTTTC TTCTGGTCCC CTCTAATCTA AAGCGAGTTC CTTTTTCAGC AGAAACTGTC
    TTGCCTCTGT CCAGACCCTC TGTGCCAGAT GTGCTGGCAA CTGAACAGGA CATCCAGACT
    GAGGTTCTTG TTCATCTGAC TGGGCAGACC CCAGTTGTCT CTGACTGGGC ATCAGTGGAT
    GAACCCAAGG AAAAGCACGA GCCTATTGCT CACTTACTGG ATGGACAAGA CAAGGCAGAG
    CAAGTGCTAC CCACTTTGAG TTGCACACCT GAACCCATGA CAATGAGCTC CCCTCTTTCC
    CAAGCCAAGA TCATGCAAAC TGGAGGTGGA TATGTAAACT GGGCATTTTC AGAAGGTGAT
    GAAACTGGTG TGTTTAGCAT CAAGAAAAAG TGGCAAACCT GCTTGCCCTC CACTTGTGAC
    AGTGATTCCT CTCGGAGTGA ACAGCACCAG AAGCAGGCCC AGGACAGCTC CCTATCTGAT
    AACTCAACAA GATCGGCCCA GAGTAGTGAA TGCTCAGAGG TGGGACCATG GCTTCAGCCA
    AACACATCCT TTTGGATCAA TCCTCTCCGC AGATACAGGC CCTTCGCTAG GAGTCATAGT
    TTTAGATTCC ATAAGGAGGA GAAATTGATG AAGATCTGTA AGATTAAAAA TCTTTCAGGC
    TCTTCAGAAA TAGGGCAGGG AGCATGGGTC AAAGCGAAAA TGCTAACCAA AGACAGGAGA
    CTGTCAAAGA AAAAGAAGAA TACTCAAGGA CTCCAGGTGC CAATCATAAC AGTCAATGCC
    TGCTCTCAGA GTGACCAGTT GAATCCAGAG CCAGGAGAAA ACAGCATCTC TGAAGAGGAG
    TACAGCAAGA ACTGGTTCAC AGTGTCCAAA TTTAGTCACA CAGGTGTAGA ACCTTACATA
    CATCAGAAAA TGAAAACTAA AGAAATTGGA CAATGTGCTA TACAAATCAG TGATTACCTA
    AAGCAGTCTC AAGAGGATCT CAGCAAAAAC TCTTTGTGGA ATTCCAGGAG CACCAACCTC
    AATAGGAACT CCCTGCTGAA AAGTTCAATT GGAGTTGACA AGATCTCAGC CTCCTTAAAA
    AGCCCTCAAG AGCCTCACCA TCATTATTCA GCCATTGAAA GGAATAATTT AATGAGGCTT
    TCTCAGACCA TACCATTTAC ACCAGTCCAA CTGTTTGCAG GAGAAGAAAT AACTGTCTAC
    AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT
    GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA
    GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC
    ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC
    ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC
    TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT
    TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG
    GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG
    GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT
    TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC
    AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC
    TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA
    AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA
    TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA
    CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98450
    Clone ID Related Accession (Same CDS sequence) XM_017014288.1
    Accession Version XM_017014288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA
    AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC
    CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG
    AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC
    GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG
    GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC
    TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA
    CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC
    TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA
    ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG
    ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA
    TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGATC
    TGTATTTCAG AACCTGGGAA GTTTACCCAA AAGGTGAAGG TATGGATTAG TGAGTACTGG
    AACTTAACAG AAACTGTGGC CATTGGCCTG TTTTCAGCTG GCTTCGTCCT TCGATGGGGT
    GACCCTCCTT TTCACACAGC GGGAAGACTG ATCTACTGCA TAGACATCAT ATTCTGGTTC
    TCACGGCTCC TGGACTTCTT TGCTGTGAAT CAACATGCAG GTCCATATGT GACCATGATT
    GCAAAAATGA CAGCAAACAT GTTCTATATT GTGATCATCA TGGCCATAGT CCTGCTGAGC
    TTTGGAGTGG CACGCAAGGC CATCCTTTCG CCAAAAGAGC CACCATCTTG GAGTCTAGCT
    CGAGATATTG TATTTGAGCC ATACTGGATG ATATACGGAG AAGTCTATGC TGGAGAAATA
    GATGTTTGTT CAAGCCAGCC ATCCTGCCCT CCTGGTTCTT TTCTTACTCC ATTCTTGCAA
    GCTGTCTACC TCTTCGTGCA ATATATCATC ATGGTGAACC TGTTGATTGC TTTCTTCAAC
    AACGTTTACT TAGATATGGA ATCCATTTCA AATAACCTGT GGAAATACAA CCGCTATCGC
    TACATCATGA CCTACCACGA GAAGCCCTGG CTGCCCCCAC CTCTCATCCT GCTGAGCCAC
    GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT CATCGAGCTC CTCACGACCA AGAAGAGGGT
    GACGTTGGAT TAAAACTCTA CCTCAGTAAG GAGGATCTGA AAAAACTTCA TGATTTTGAG
    GAGCAGTGCG TGGAAAAATA CTTCCATGAG AAGATGGAAG ATGTGAATTG TAGTTGTGAG
    GAACGAATCC GAGTGACATC AGAAAGGGTT ACAGAGATGT ACTTCCAGCT GAAAGAAATG
    AATGAAAAGG TGTCTTTTAT AAAGGACTCC TTACTGTCTT TGGACAGCCA GGTGGGACAC
    CTGCAGGATC TCTCTGCCCT GACTGTGGAT ACCCTGAAAG TCCTTTCTGC TGTTGACACT
    TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG AGAAAGCATT CTACTTGCAA AAAACTTCCC
    CACAGCTGGA GCAATGTCAT CTGTGCAGAG GTTCTAGGCA GCATGGAGAT CGCTGGAGAG
    AAGAAATACC AGTATTATAG CATGCCCTCT TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG
    CATCCCCCAA GAGTGCAGAG GGGGGCACTT CTTGAGATTA CAAACAGTAA AAGAGAGGCT
    ACAAATGTAA GAAATGACCA GGAAAGGCAA GAAACACAAA GTAGTATAGT GGTTTCTGGG
    GTGTCTCCTA ACAGGCAAGC ACACTCAAAG TATGGCCAGT TTCTTCTGGT CCCCTCTAAT
    CTAAAGCGAG TTCCTTTTTC AGCAGAAACT GTCTTGCCTC TGTCCAGACC CTCTGTGCCA
    GATGTGCTGG CAACTGAACA GGACATCCAG ACTGAGGTTC TTGTTCATCT GACTGGGCAG
    ACCCCAGTTG TCTCTGACTG GGCATCAGTG GATGAACCCA AGGAAAAGCA CGAGCCTATT
    GCTCACTTAC TGGATGGACA AGACAAGGCA GAGCAAGTGC TACCCACTTT GAGTTGCACA
    CCTGAACCCA TGACAATGAG CTCCCCTCTT TCCCAAGCCA AGATCATGCA AACTGGAGGT
    GGATATGTAA ACTGGGCATT TTCAGAAGGT GATGAAACTG GTGTGTTTAG CATCAAGAAA
    AAGTGGCAAA CCTGCTTGCC CTCCACTTGT GACAGTGATT CCTCTCGGAG TGAACAGCAC
    CAGAAGCAGG CCCAGGACAG CTCCCTATCT GATAACTCAA CAAGATCGGC CCAGAGTAGT
    GAATGCTCAG AGGTGGGACC ATGGCTTCAG CCAAACACAT CCTTTTGGAT CAATCCTCTC
    CGCAGATACA GGCCCTTCGC TAGGAGTCAT AGTTTTAGAT TCCATAAGGA GGAGAAATTG
    ATGAAGATCT GTAAGATTAA AAATCTTTCA GGCTCTTCAG AAATAGGGCA GGGAGCATGG
    GTCAAAGCGA AAATGCTAAC CAAAGACAGG AGACTGTCAA AGAAAAAGAA GAATACTCAA
    GGACTCCAGG TGCCAATCAT AACAGTCAAT GCCTGCTCTC AGAGTGACCA GTTGAATCCA
    GAGCCAGGAG AAAACAGCAT CTCTGAAGAG GAGTACAGCA AGAACTGGTT CACAGTGTCC
    AAATTTAGTC ACACAGGTGT AGAACCTTAC ATACATCAGA AAATGAAAAC TAAAGAAATT
    GGACAATGTG CTATACAAAT CAGTGATTAC CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA
    AACTCTTTGT GGAATTCCAG GAGCACCAAC CTCAATAGGA ACTCCCTGCT GAAAAGTTCA
    ATTGGAGTTG ACAAGATCTC AGCCTCCTTA AAAAGCCCTC AAGAGCCTCA CCATCATTAT
    TCAGCCATTG AAAGGAATAA TTTAATGAGG CTTTCTCAGA CCATACCATT TACACCAGTC
    CAACTGTTTG CAGGAGAAGA AATAACTGTC TACAGGTTGG AGGAGAGTTC CCCTTTAAAC
    CTTGATAAAA GCATGTCCTC TTGGTCTCAG CGTGGGAGAG CGGCAATGAT CCAGGTATTG
    TCCCGAGAGG AGATGGATGG GGGCCTCCGT AAAGCTATGA GAGTCGTCAG CACTTGGTCT
    GAGGATGACA TTCTCAAGCC GGGACAAGTT TTCATTGTCA AGTCCTTTCT TCCTGAGGTT
    GTGCGGACAT GGCATAAAAT CTTCCAGGAG AGCACTGTGC TTCATCTTTG CCTCAGGGAA
    ATTCAACAAC AAAGAGCTGC TCAAAAATTG ATCTATACCT TCAACCAAGT GAAACCACAA
    ACCATACCCT ACACACCAAG GTTCCTGGAA GTTTTCTTAA TCTACTGCCA TTCAGCCAAC
    CAGTGGTTGA CCATTGAGAA GTATATGACA GGGGAGTTCC GGAAGTATAA CAACAACAAT
    GGTGATGAAA TCACCCCCAC CAACACCCTG GAGGAGCTGA TGTTGGCTTT CTCTCACTGG
    ACCTATGAGT ACACTCGGGG AGAGCTGCTG GTTTTAGATT TGCAAGGTGT TGGAGAAAAT
    TTGACAGATC CATCTGTTAT AAAACCTGAA GTCAAACAAT CAAGAGGAAT GGTGTTTGGA
    CCGGCCAATT TGGGGGAAGA TGCAATTAGA AACTTCATTG CAAAACATCA TTGTAACTCC
    TGCTGCCGGA AGCTCAAACT CCCGGATTTA AAAAGAAATG ACTATTCCCC TGAAAGGATA
    AATTCCACCT TTGGACTTGA GATAAAAATA GAATCAGCTG AGGAGCCTCC AGCAAGGGAG
    ACGGGTAGAA ATTCCCCAGA AGATGATATG CAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869777.1
    CDS239..5797
    Translation

    Target ORF information:

    RefSeq Version XM_017014288.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA
    AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC
    CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG
    AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC
    GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG
    GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC
    TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA
    CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC
    TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA
    ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG
    ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA
    TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGATC
    TGTATTTCAG AACCTGGGAA GTTTACCCAA AAGGTGAAGG TATGGATTAG TGAGTACTGG
    AACTTAACAG AAACTGTGGC CATTGGCCTG TTTTCAGCTG GCTTCGTCCT TCGATGGGGT
    GACCCTCCTT TTCACACAGC GGGAAGACTG ATCTACTGCA TAGACATCAT ATTCTGGTTC
    TCACGGCTCC TGGACTTCTT TGCTGTGAAT CAACATGCAG GTCCATATGT GACCATGATT
    GCAAAAATGA CAGCAAACAT GTTCTATATT GTGATCATCA TGGCCATAGT CCTGCTGAGC
    TTTGGAGTGG CACGCAAGGC CATCCTTTCG CCAAAAGAGC CACCATCTTG GAGTCTAGCT
    CGAGATATTG TATTTGAGCC ATACTGGATG ATATACGGAG AAGTCTATGC TGGAGAAATA
    GATGTTTGTT CAAGCCAGCC ATCCTGCCCT CCTGGTTCTT TTCTTACTCC ATTCTTGCAA
    GCTGTCTACC TCTTCGTGCA ATATATCATC ATGGTGAACC TGTTGATTGC TTTCTTCAAC
    AACGTTTACT TAGATATGGA ATCCATTTCA AATAACCTGT GGAAATACAA CCGCTATCGC
    TACATCATGA CCTACCACGA GAAGCCCTGG CTGCCCCCAC CTCTCATCCT GCTGAGCCAC
    GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT CATCGAGCTC CTCACGACCA AGAAGAGGGT
    GACGTTGGAT TAAAACTCTA CCTCAGTAAG GAGGATCTGA AAAAACTTCA TGATTTTGAG
    GAGCAGTGCG TGGAAAAATA CTTCCATGAG AAGATGGAAG ATGTGAATTG TAGTTGTGAG
    GAACGAATCC GAGTGACATC AGAAAGGGTT ACAGAGATGT ACTTCCAGCT GAAAGAAATG
    AATGAAAAGG TGTCTTTTAT AAAGGACTCC TTACTGTCTT TGGACAGCCA GGTGGGACAC
    CTGCAGGATC TCTCTGCCCT GACTGTGGAT ACCCTGAAAG TCCTTTCTGC TGTTGACACT
    TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG AGAAAGCATT CTACTTGCAA AAAACTTCCC
    CACAGCTGGA GCAATGTCAT CTGTGCAGAG GTTCTAGGCA GCATGGAGAT CGCTGGAGAG
    AAGAAATACC AGTATTATAG CATGCCCTCT TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG
    CATCCCCCAA GAGTGCAGAG GGGGGCACTT CTTGAGATTA CAAACAGTAA AAGAGAGGCT
    ACAAATGTAA GAAATGACCA GGAAAGGCAA GAAACACAAA GTAGTATAGT GGTTTCTGGG
    GTGTCTCCTA ACAGGCAAGC ACACTCAAAG TATGGCCAGT TTCTTCTGGT CCCCTCTAAT
    CTAAAGCGAG TTCCTTTTTC AGCAGAAACT GTCTTGCCTC TGTCCAGACC CTCTGTGCCA
    GATGTGCTGG CAACTGAACA GGACATCCAG ACTGAGGTTC TTGTTCATCT GACTGGGCAG
    ACCCCAGTTG TCTCTGACTG GGCATCAGTG GATGAACCCA AGGAAAAGCA CGAGCCTATT
    GCTCACTTAC TGGATGGACA AGACAAGGCA GAGCAAGTGC TACCCACTTT GAGTTGCACA
    CCTGAACCCA TGACAATGAG CTCCCCTCTT TCCCAAGCCA AGATCATGCA AACTGGAGGT
    GGATATGTAA ACTGGGCATT TTCAGAAGGT GATGAAACTG GTGTGTTTAG CATCAAGAAA
    AAGTGGCAAA CCTGCTTGCC CTCCACTTGT GACAGTGATT CCTCTCGGAG TGAACAGCAC
    CAGAAGCAGG CCCAGGACAG CTCCCTATCT GATAACTCAA CAAGATCGGC CCAGAGTAGT
    GAATGCTCAG AGGTGGGACC ATGGCTTCAG CCAAACACAT CCTTTTGGAT CAATCCTCTC
    CGCAGATACA GGCCCTTCGC TAGGAGTCAT AGTTTTAGAT TCCATAAGGA GGAGAAATTG
    ATGAAGATCT GTAAGATTAA AAATCTTTCA GGCTCTTCAG AAATAGGGCA GGGAGCATGG
    GTCAAAGCGA AAATGCTAAC CAAAGACAGG AGACTGTCAA AGAAAAAGAA GAATACTCAA
    GGACTCCAGG TGCCAATCAT AACAGTCAAT GCCTGCTCTC AGAGTGACCA GTTGAATCCA
    GAGCCAGGAG AAAACAGCAT CTCTGAAGAG GAGTACAGCA AGAACTGGTT CACAGTGTCC
    AAATTTAGTC ACACAGGTGT AGAACCTTAC ATACATCAGA AAATGAAAAC TAAAGAAATT
    GGACAATGTG CTATACAAAT CAGTGATTAC CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA
    AACTCTTTGT GGAATTCCAG GAGCACCAAC CTCAATAGGA ACTCCCTGCT GAAAAGTTCA
    ATTGGAGTTG ACAAGATCTC AGCCTCCTTA AAAAGCCCTC AAGAGCCTCA CCATCATTAT
    TCAGCCATTG AAAGGAATAA TTTAATGAGG CTTTCTCAGA CCATACCATT TACACCAGTC
    CAACTGTTTG CAGGAGAAGA AATAACTGTC TACAGGTTGG AGGAGAGTTC CCCTTTAAAC
    CTTGATAAAA GCATGTCCTC TTGGTCTCAG CGTGGGAGAG CGGCAATGAT CCAGGTATTG
    TCCCGAGAGG AGATGGATGG GGGCCTCCGT AAAGCTATGA GAGTCGTCAG CACTTGGTCT
    GAGGATGACA TTCTCAAGCC GGGACAAGTT TTCATTGTCA AGTCCTTTCT TCCTGAGGTT
    GTGCGGACAT GGCATAAAAT CTTCCAGGAG AGCACTGTGC TTCATCTTTG CCTCAGGGAA
    ATTCAACAAC AAAGAGCTGC TCAAAAATTG ATCTATACCT TCAACCAAGT GAAACCACAA
    ACCATACCCT ACACACCAAG GTTCCTGGAA GTTTTCTTAA TCTACTGCCA TTCAGCCAAC
    CAGTGGTTGA CCATTGAGAA GTATATGACA GGGGAGTTCC GGAAGTATAA CAACAACAAT
    GGTGATGAAA TCACCCCCAC CAACACCCTG GAGGAGCTGA TGTTGGCTTT CTCTCACTGG
    ACCTATGAGT ACACTCGGGG AGAGCTGCTG GTTTTAGATT TGCAAGGTGT TGGAGAAAAT
    TTGACAGATC CATCTGTTAT AAAACCTGAA GTCAAACAAT CAAGAGGAAT GGTGTTTGGA
    CCGGCCAATT TGGGGGAAGA TGCAATTAGA AACTTCATTG CAAAACATCA TTGTAACTCC
    TGCTGCCGGA AGCTCAAACT CCCGGATTTA AAAAGAAATG ACTATTCCCC TGAAAGGATA
    AATTCCACCT TTGGACTTGA GATAAAAATA GAATCAGCTG AGGAGCCTCC AGCAAGGGAG
    ACGGGTAGAA ATTCCCCAGA AGATGATATG CAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98451
    Clone ID Related Accession (Same CDS sequence) XM_017014289.1
    Accession Version XM_017014289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAGTAAGGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869778.1
    CDS239..4921
    Translation

    Target ORF information:

    RefSeq Version XM_017014289.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAGTAAGGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75940
    Clone ID Related Accession (Same CDS sequence) XM_011518245.1
    Accession Version XM_011518245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5976bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGGA AAAGTCTATA GTCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAACAAAATG TATCAGATGA CCCTGAGTCT ACTGGCTTTC TTTACCCTTA CAATGACCTG
    CTGGTTTGGG CTGTGCTGAT GAAAAGGCAG AAGATGGCTA TGTTCTTCTG GCAGCATGGA
    GAGGAGGCCA CGGTTAAAGC CGTGATTGCG TGTATCCTCT ACCGGGCAAT GGCCCATGAA
    GCTAAGGAGA GTCACATGGT GGATGATGCC TCAGAAGAGT TGAAGAATTA CTCAAAACAG
    TTTGGCCAGC TGGCTCTGGA CTTGTTGGAG AAGGCATTCA AGCAGAATGA GCGCATGGCC
    ATGACGCTGT TGACGTATGA ACTCAGGAAC TGGAGCAATT CGACCTGCCT TAAACTGGCC
    GTGTCGGGAG GATTACGACC CTTTGTTTCA CATACTTGTA CCCAGATGCT ACTGACAGAC
    ATGTGGATGG GGAGGCTGAA AATGAGGAAA AACTCTTGGT TAAAGATTAT TATAAGCATT
    ATTTTACCAC CCACCATTTT GACACTGGAA TTTAAAAGCA AAGCTGAGAT GTCACATGTT
    CCCCAGTCCC AGGACTTCCA ATTTATGTGG TATTACAGTG ACCAGAACGC CAGCAGTTCC
    AAAGAAAGTG CTTCTGTGAA AGAGTATGAT TTGGAAAGGG GCCATGATGA GAAACTGGAT
    GAAAATCAGC ATTTTGGTTT GGAAAGTGGG CACCAACACC TTCCGTGGAC CAGGAAAGTC
    TATGAGTTCT ACAGTGCTCC AATTGTCAAG TTTTGGTTTT ATACGATGGC GTATTTGGCA
    TTCCTCATGC TGTTCACTTA CACCGTGTTG GTGGAGATGC AGCCCCAGCC CAGCGTGCAG
    GAGTGGCTTG TTAGCATTTA CATCTTCACC AATGCTATTG AGGTGGTCAG GGAGATCTGT
    ATTTCAGAAC CTGGGAAGTT TACCCAAAAG GTGAAGGTAT GGATTAGTGA GTACTGGAAC
    TTAACAGAAA CTGTGGCCAT TGGCCTGTTT TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC
    CCTCCTTTTC ACACAGCGGG AAGACTGATC TACTGCATAG ACATCATATT CTGGTTCTCA
    CGGCTCCTGG ACTTCTTTGC TGTGAATCAA CATGCAGGTC CATATGTGAC CATGATTGCA
    AAAATGACAG CAAACATGTT CTATATTGTG ATCATCATGG CCATAGTCCT GCTGAGCTTT
    GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA AAAGAGCCAC CATCTTGGAG TCTAGCTCGA
    GATATTGTAT TTGAGCCATA CTGGATGATA TACGGAGAAG TCTATGCTGG AGAAATAGAT
    GTTTGTTCAA GCCAGCCATC CTGCCCTCCT GGTTCTTTTC TTACTCCATT CTTGCAAGCT
    GTCTACCTCT TCGTGCAATA TATCATCATG GTGAACCTGT TGATTGCTTT CTTCAACAAC
    GTTTACTTAG ATATGGAATC CATTTCAAAT AACCTGTGGA AATACAACCG CTATCGCTAC
    ATCATGACCT ACCACGAGAA GCCCTGGCTG CCCCCACCTC TCATCCTGCT GAGCCACGTG
    GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT CGAGCTCCTC ACGACCAAGA AGAGGGTGAC
    GTTGGATTAA AACTCTACCT CAGTAAGGAG GATCTGAAAA AACTTCATGA TTTTGAGGAG
    CAGTGCGTGG AAAAATACTT CCATGAGAAG ATGGAAGATG TGAATTGTAG TTGTGAGGAA
    CGAATCCGAG TGACATCAGA AAGGGTTACA GAGATGTACT TCCAGCTGAA AGAAATGAAT
    GAAAAGGTGT CTTTTATAAA GGACTCCTTA CTGTCTTTGG ACAGCCAGGT GGGACACCTG
    CAGGATCTCT CTGCCCTGAC TGTGGATACC CTGAAAGTCC TTTCTGCTGT TGACACTTTG
    CAAGAGGATG AGGCTCTCCT GGCCAAGAGA AAGCATTCTA CTTGCAAAAA ACTTCCCCAC
    AGCTGGAGCA ATGTCATCTG TGCAGAGGTT CTAGGCAGCA TGGAGATCGC TGGAGAGAAG
    AAATACCAGT ATTATAGCAT GCCCTCTTCT TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT
    CCCCCAAGAG TGCAGAGGGG GGCACTTCTT GAGATTACAA ACAGTAAAAG AGAGGCTACA
    AATGTAAGAA ATGACCAGGA AAGGCAAGAA ACACAAAGTA GTATAGTGGT TTCTGGGGTG
    TCTCCTAACA GGCAAGCACA CTCAAAGTAT GGCCAGTTTC TTCTGGTCCC CTCTAATCTA
    AAGCGAGTTC CTTTTTCAGC AGAAACTGTC TTGCCTCTGT CCAGACCCTC TGTGCCAGAT
    GTGCTGGCAA CTGAACAGGA CATCCAGACT GAGGTTCTTG TTCATCTGAC TGGGCAGACC
    CCAGTTGTCT CTGACTGGGC ATCAGTGGAT GAACCCAAGG AAAAGCACGA GCCTATTGCT
    CACTTACTGG ATGGACAAGA CAAGGCAGAG CAAGTGCTAC CCACTTTGAG TTGCACACCT
    GAACCCATGA CAATGAGCTC CCCTCTTTCC CAAGCCAAGA TCATGCAAAC TGGAGGTGGA
    TATGTAAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAT CAAGAAAAAG
    TGGCAAACCT GCTTGCCCTC CACTTGTGAC AGTGATTCCT CTCGGAGTGA ACAGCACCAG
    AAGCAGGCCC AGGACAGCTC CCTATCTGAT AACTCAACAA GATCGGCCCA GAGTAGTGAA
    TGCTCAGAGG TGGGACCATG GCTTCAGCCA AACACATCCT TTTGGATCAA TCCTCTCCGC
    AGATACAGGC CCTTCGCTAG GAGTCATAGT TTTAGATTCC ATAAGGAGGA GAAATTGATG
    AAGATCTGTA AGATTAAAAA TCTTTCAGGC TCTTCAGAAA TAGGGCAGGG AGCATGGGTC
    AAAGCGAAAA TGCTAACCAA AGACAGGAGA CTGTCAAAGA AAAAGAAGAA TACTCAAGGA
    CTCCAGGTGC CAATCATAAC AGTCAATGCC TGCTCTCAGA GTGACCAGTT GAATCCAGAG
    CCAGGAGAAA ACAGCATCTC TGAAGAGGAG TACAGCAAGA ACTGGTTCAC AGTGTCCAAA
    TTTAGTCACA CAGGTGTAGA ACCTTACATA CATCAGAAAA TGAAAACTAA AGAAATTGGA
    CAATGTGCTA TACAAATCAG TGATTACCTA AAGCAGTCTC AAGAGGATCT CAGCAAAAAC
    TCTTTGTGGA ATTCCAGGAG CACCAACCTC AATAGGAACT CCCTGCTGAA AAGTTCAATT
    GGAGTTGACA AGATCTCAGC CTCCTTAAAA AGCCCTCAAG AGCCTCACCA TCATTATTCA
    GCCATTGAAA GGAATAATTT AATGAGGCTT TCTCAGACCA TACCATTTAC ACCAGTCCAA
    CTGTTTGCAG GAGAAGAAAT AACTGTCTAC AGGTTGGAGG AGAGTTCCCC TTTAAACCTT
    GATAAAAGCA TGTCCTCTTG GTCTCAGCGT GGGAGAGCGG CAATGATCCA GGTATTGTCC
    CGAGAGGAGA TGGATGGGGG CCTCCGTAAA GCTATGAGAG TCGTCAGCAC TTGGTCTGAG
    GATGACATTC TCAAGCCGGG ACAAGTTTTC ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG
    CGGACATGGC ATAAAATCTT CCAGGAGAGC ACTGTGCTTC ATCTTTGCCT CAGGGAAATT
    CAACAACAAA GAGCTGCTCA AAAATTGATC TATACCTTCA ACCAAGTGAA ACCACAAACC
    ATACCCTACA CACCAAGGTT CCTGGAAGTT TTCTTAATCT ACTGCCATTC AGCCAACCAG
    TGGTTGACCA TTGAGAAGTA TATGACAGGG GAGTTCCGGA AGTATAACAA CAACAATGGT
    GATGAAATCA CCCCCACCAA CACCCTGGAG GAGCTGATGT TGGCTTTCTC TCACTGGACC
    TATGAGTACA CTCGGGGAGA GCTGCTGGTT TTAGATTTGC AAGGTGTTGG AGAAAATTTG
    ACAGATCCAT CTGTTATAAA ACCTGAAGTC AAACAATCAA GAGGAATGGT GTTTGGACCG
    GCCAATTTGG GGGAAGATGC AATTAGAAAC TTCATTGCAA AACATCATTG TAACTCCTGC
    TGCCGGAAGC TCAAACTCCC GGATTTAAAA AGAAATGACT ATTCCCCTGA AAGGATAAAT
    TCCACCTTTG GACTTGAGAT AAAAATAGAA TCAGCTGAGG AGCCTCCAGC AAGGGAGACG
    GGTAGAAATT CCCCAGAAGA TGATATGCAA CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516547.1
    CDS239..6214
    Translation

    Target ORF information:

    RefSeq Version XM_011518245.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGGA AAAGTCTATA GTCCTTCATA AATCAAGGAA GAAGTCAAAA
    GAACAAAATG TATCAGATGA CCCTGAGTCT ACTGGCTTTC TTTACCCTTA CAATGACCTG
    CTGGTTTGGG CTGTGCTGAT GAAAAGGCAG AAGATGGCTA TGTTCTTCTG GCAGCATGGA
    GAGGAGGCCA CGGTTAAAGC CGTGATTGCG TGTATCCTCT ACCGGGCAAT GGCCCATGAA
    GCTAAGGAGA GTCACATGGT GGATGATGCC TCAGAAGAGT TGAAGAATTA CTCAAAACAG
    TTTGGCCAGC TGGCTCTGGA CTTGTTGGAG AAGGCATTCA AGCAGAATGA GCGCATGGCC
    ATGACGCTGT TGACGTATGA ACTCAGGAAC TGGAGCAATT CGACCTGCCT TAAACTGGCC
    GTGTCGGGAG GATTACGACC CTTTGTTTCA CATACTTGTA CCCAGATGCT ACTGACAGAC
    ATGTGGATGG GGAGGCTGAA AATGAGGAAA AACTCTTGGT TAAAGATTAT TATAAGCATT
    ATTTTACCAC CCACCATTTT GACACTGGAA TTTAAAAGCA AAGCTGAGAT GTCACATGTT
    CCCCAGTCCC AGGACTTCCA ATTTATGTGG TATTACAGTG ACCAGAACGC CAGCAGTTCC
    AAAGAAAGTG CTTCTGTGAA AGAGTATGAT TTGGAAAGGG GCCATGATGA GAAACTGGAT
    GAAAATCAGC ATTTTGGTTT GGAAAGTGGG CACCAACACC TTCCGTGGAC CAGGAAAGTC
    TATGAGTTCT ACAGTGCTCC AATTGTCAAG TTTTGGTTTT ATACGATGGC GTATTTGGCA
    TTCCTCATGC TGTTCACTTA CACCGTGTTG GTGGAGATGC AGCCCCAGCC CAGCGTGCAG
    GAGTGGCTTG TTAGCATTTA CATCTTCACC AATGCTATTG AGGTGGTCAG GGAGATCTGT
    ATTTCAGAAC CTGGGAAGTT TACCCAAAAG GTGAAGGTAT GGATTAGTGA GTACTGGAAC
    TTAACAGAAA CTGTGGCCAT TGGCCTGTTT TCAGCTGGCT TCGTCCTTCG ATGGGGTGAC
    CCTCCTTTTC ACACAGCGGG AAGACTGATC TACTGCATAG ACATCATATT CTGGTTCTCA
    CGGCTCCTGG ACTTCTTTGC TGTGAATCAA CATGCAGGTC CATATGTGAC CATGATTGCA
    AAAATGACAG CAAACATGTT CTATATTGTG ATCATCATGG CCATAGTCCT GCTGAGCTTT
    GGAGTGGCAC GCAAGGCCAT CCTTTCGCCA AAAGAGCCAC CATCTTGGAG TCTAGCTCGA
    GATATTGTAT TTGAGCCATA CTGGATGATA TACGGAGAAG TCTATGCTGG AGAAATAGAT
    GTTTGTTCAA GCCAGCCATC CTGCCCTCCT GGTTCTTTTC TTACTCCATT CTTGCAAGCT
    GTCTACCTCT TCGTGCAATA TATCATCATG GTGAACCTGT TGATTGCTTT CTTCAACAAC
    GTTTACTTAG ATATGGAATC CATTTCAAAT AACCTGTGGA AATACAACCG CTATCGCTAC
    ATCATGACCT ACCACGAGAA GCCCTGGCTG CCCCCACCTC TCATCCTGCT GAGCCACGTG
    GGCCTTCTCC TCCGCCGCCT GTGCTGTCAT CGAGCTCCTC ACGACCAAGA AGAGGGTGAC
    GTTGGATTAA AACTCTACCT CAGTAAGGAG GATCTGAAAA AACTTCATGA TTTTGAGGAG
    CAGTGCGTGG AAAAATACTT CCATGAGAAG ATGGAAGATG TGAATTGTAG TTGTGAGGAA
    CGAATCCGAG TGACATCAGA AAGGGTTACA GAGATGTACT TCCAGCTGAA AGAAATGAAT
    GAAAAGGTGT CTTTTATAAA GGACTCCTTA CTGTCTTTGG ACAGCCAGGT GGGACACCTG
    CAGGATCTCT CTGCCCTGAC TGTGGATACC CTGAAAGTCC TTTCTGCTGT TGACACTTTG
    CAAGAGGATG AGGCTCTCCT GGCCAAGAGA AAGCATTCTA CTTGCAAAAA ACTTCCCCAC
    AGCTGGAGCA ATGTCATCTG TGCAGAGGTT CTAGGCAGCA TGGAGATCGC TGGAGAGAAG
    AAATACCAGT ATTATAGCAT GCCCTCTTCT TTGCTGAGGA GCCTGGCTGG AGGCCGGCAT
    CCCCCAAGAG TGCAGAGGGG GGCACTTCTT GAGATTACAA ACAGTAAAAG AGAGGCTACA
    AATGTAAGAA ATGACCAGGA AAGGCAAGAA ACACAAAGTA GTATAGTGGT TTCTGGGGTG
    TCTCCTAACA GGCAAGCACA CTCAAAGTAT GGCCAGTTTC TTCTGGTCCC CTCTAATCTA
    AAGCGAGTTC CTTTTTCAGC AGAAACTGTC TTGCCTCTGT CCAGACCCTC TGTGCCAGAT
    GTGCTGGCAA CTGAACAGGA CATCCAGACT GAGGTTCTTG TTCATCTGAC TGGGCAGACC
    CCAGTTGTCT CTGACTGGGC ATCAGTGGAT GAACCCAAGG AAAAGCACGA GCCTATTGCT
    CACTTACTGG ATGGACAAGA CAAGGCAGAG CAAGTGCTAC CCACTTTGAG TTGCACACCT
    GAACCCATGA CAATGAGCTC CCCTCTTTCC CAAGCCAAGA TCATGCAAAC TGGAGGTGGA
    TATGTAAACT GGGCATTTTC AGAAGGTGAT GAAACTGGTG TGTTTAGCAT CAAGAAAAAG
    TGGCAAACCT GCTTGCCCTC CACTTGTGAC AGTGATTCCT CTCGGAGTGA ACAGCACCAG
    AAGCAGGCCC AGGACAGCTC CCTATCTGAT AACTCAACAA GATCGGCCCA GAGTAGTGAA
    TGCTCAGAGG TGGGACCATG GCTTCAGCCA AACACATCCT TTTGGATCAA TCCTCTCCGC
    AGATACAGGC CCTTCGCTAG GAGTCATAGT TTTAGATTCC ATAAGGAGGA GAAATTGATG
    AAGATCTGTA AGATTAAAAA TCTTTCAGGC TCTTCAGAAA TAGGGCAGGG AGCATGGGTC
    AAAGCGAAAA TGCTAACCAA AGACAGGAGA CTGTCAAAGA AAAAGAAGAA TACTCAAGGA
    CTCCAGGTGC CAATCATAAC AGTCAATGCC TGCTCTCAGA GTGACCAGTT GAATCCAGAG
    CCAGGAGAAA ACAGCATCTC TGAAGAGGAG TACAGCAAGA ACTGGTTCAC AGTGTCCAAA
    TTTAGTCACA CAGGTGTAGA ACCTTACATA CATCAGAAAA TGAAAACTAA AGAAATTGGA
    CAATGTGCTA TACAAATCAG TGATTACCTA AAGCAGTCTC AAGAGGATCT CAGCAAAAAC
    TCTTTGTGGA ATTCCAGGAG CACCAACCTC AATAGGAACT CCCTGCTGAA AAGTTCAATT
    GGAGTTGACA AGATCTCAGC CTCCTTAAAA AGCCCTCAAG AGCCTCACCA TCATTATTCA
    GCCATTGAAA GGAATAATTT AATGAGGCTT TCTCAGACCA TACCATTTAC ACCAGTCCAA
    CTGTTTGCAG GAGAAGAAAT AACTGTCTAC AGGTTGGAGG AGAGTTCCCC TTTAAACCTT
    GATAAAAGCA TGTCCTCTTG GTCTCAGCGT GGGAGAGCGG CAATGATCCA GGTATTGTCC
    CGAGAGGAGA TGGATGGGGG CCTCCGTAAA GCTATGAGAG TCGTCAGCAC TTGGTCTGAG
    GATGACATTC TCAAGCCGGG ACAAGTTTTC ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG
    CGGACATGGC ATAAAATCTT CCAGGAGAGC ACTGTGCTTC ATCTTTGCCT CAGGGAAATT
    CAACAACAAA GAGCTGCTCA AAAATTGATC TATACCTTCA ACCAAGTGAA ACCACAAACC
    ATACCCTACA CACCAAGGTT CCTGGAAGTT TTCTTAATCT ACTGCCATTC AGCCAACCAG
    TGGTTGACCA TTGAGAAGTA TATGACAGGG GAGTTCCGGA AGTATAACAA CAACAATGGT
    GATGAAATCA CCCCCACCAA CACCCTGGAG GAGCTGATGT TGGCTTTCTC TCACTGGACC
    TATGAGTACA CTCGGGGAGA GCTGCTGGTT TTAGATTTGC AAGGTGTTGG AGAAAATTTG
    ACAGATCCAT CTGTTATAAA ACCTGAAGTC AAACAATCAA GAGGAATGGT GTTTGGACCG
    GCCAATTTGG GGGAAGATGC AATTAGAAAC TTCATTGCAA AACATCATTG TAACTCCTGC
    TGCCGGAAGC TCAAACTCCC GGATTTAAAA AGAAATGACT ATTCCCCTGA AAGGATAAAT
    TCCACCTTTG GACTTGAGAT AAAAATAGAA TCAGCTGAGG AGCCTCCAGC AAGGGAGACG
    GGTAGAAATT CCCCAGAAGA TGATATGCAA CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75941
    Clone ID Related Accession (Same CDS sequence) XM_011518246.1
    Accession Version XM_011518246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC
    TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG
    CGTGGGAGAG CGGCAATGAT CCAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC
    ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC
    TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT
    TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG
    GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG
    GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT
    TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC
    AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC
    TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA
    AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA
    TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA
    CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516548.1
    CDS239..6184
    Translation

    Target ORF information:

    RefSeq Version XM_011518246.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG CAGGAGAAGA AATAACTGTC
    TACAGGTTGG AGGAGAGTTC CCCTTTAAAC CTTGATAAAA GCATGTCCTC TTGGTCTCAG
    CGTGGGAGAG CGGCAATGAT CCAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC
    ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC
    TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT
    TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG
    GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG
    GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT
    TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC
    AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC
    TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA
    AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA
    TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA
    CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75942
    Clone ID Related Accession (Same CDS sequence) XM_011518247.1
    Accession Version XM_011518247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG
    GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT
    GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC
    ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA
    GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC
    ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG
    CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA
    GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT
    TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC
    CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG
    TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA
    CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT
    CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG
    AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA
    GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG
    TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT
    TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA
    GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT
    TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC
    AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG
    AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT
    ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA
    AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG
    TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT
    CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT
    CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC
    AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG
    CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC
    AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT
    GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT
    TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA
    ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA
    TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA
    TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA
    GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA
    AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT
    CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC
    AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG
    AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG
    TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG
    AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT
    CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG
    ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG
    GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA
    GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG
    AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC
    AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG
    CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC
    TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA
    ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC
    CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG
    ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT
    TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA
    TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT
    GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT
    GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT
    GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516549.1
    CDS239..6178
    Translation

    Target ORF information:

    RefSeq Version XM_011518247.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT CTGTATTTCA GAACCTGGGA AGTTTACCCA AAAGGTGAAG
    GTATGGATTA GTGAGTACTG GAACTTAACA GAAACTGTGG CCATTGGCCT GTTTTCAGCT
    GGCTTCGTCC TTCGATGGGG TGACCCTCCT TTTCACACAG CGGGAAGACT GATCTACTGC
    ATAGACATCA TATTCTGGTT CTCACGGCTC CTGGACTTCT TTGCTGTGAA TCAACATGCA
    GGTCCATATG TGACCATGAT TGCAAAAATG ACAGCAAACA TGTTCTATAT TGTGATCATC
    ATGGCCATAG TCCTGCTGAG CTTTGGAGTG GCACGCAAGG CCATCCTTTC GCCAAAAGAG
    CCACCATCTT GGAGTCTAGC TCGAGATATT GTATTTGAGC CATACTGGAT GATATACGGA
    GAAGTCTATG CTGGAGAAAT AGATGTTTGT TCAAGCCAGC CATCCTGCCC TCCTGGTTCT
    TTTCTTACTC CATTCTTGCA AGCTGTCTAC CTCTTCGTGC AATATATCAT CATGGTGAAC
    CTGTTGATTG CTTTCTTCAA CAACGTTTAC TTAGATATGG AATCCATTTC AAATAACCTG
    TGGAAATACA ACCGCTATCG CTACATCATG ACCTACCACG AGAAGCCCTG GCTGCCCCCA
    CCTCTCATCC TGCTGAGCCA CGTGGGCCTT CTCCTCCGCC GCCTGTGCTG TCATCGAGCT
    CCTCACGACC AAGAAGAGGG TGACGTTGGA TTAAAACTCT ACCTCAGTAA GGAGGATCTG
    AAAAAACTTC ATGATTTTGA GGAGCAGTGC GTGGAAAAAT ACTTCCATGA GAAGATGGAA
    GATGTGAATT GTAGTTGTGA GGAACGAATC CGAGTGACAT CAGAAAGGGT TACAGAGATG
    TACTTCCAGC TGAAAGAAAT GAATGAAAAG GTGTCTTTTA TAAAGGACTC CTTACTGTCT
    TTGGACAGCC AGGTGGGACA CCTGCAGGAT CTCTCTGCCC TGACTGTGGA TACCCTGAAA
    GTCCTTTCTG CTGTTGACAC TTTGCAAGAG GATGAGGCTC TCCTGGCCAA GAGAAAGCAT
    TCTACTTGCA AAAAACTTCC CCACAGCTGG AGCAATGTCA TCTGTGCAGA GGTTCTAGGC
    AGCATGGAGA TCGCTGGAGA GAAGAAATAC CAGTATTATA GCATGCCCTC TTCTTTGCTG
    AGGAGCCTGG CTGGAGGCCG GCATCCCCCA AGAGTGCAGA GGGGGGCACT TCTTGAGATT
    ACAAACAGTA AAAGAGAGGC TACAAATGTA AGAAATGACC AGGAAAGGCA AGAAACACAA
    AGTAGTATAG TGGTTTCTGG GGTGTCTCCT AACAGGCAAG CACACTCAAA GTATGGCCAG
    TTTCTTCTGG TCCCCTCTAA TCTAAAGCGA GTTCCTTTTT CAGCAGAAAC TGTCTTGCCT
    CTGTCCAGAC CCTCTGTGCC AGATGTGCTG GCAACTGAAC AGGACATCCA GACTGAGGTT
    CTTGTTCATC TGACTGGGCA GACCCCAGTT GTCTCTGACT GGGCATCAGT GGATGAACCC
    AAGGAAAAGC ACGAGCCTAT TGCTCACTTA CTGGATGGAC AAGACAAGGC AGAGCAAGTG
    CTACCCACTT TGAGTTGCAC ACCTGAACCC ATGACAATGA GCTCCCCTCT TTCCCAAGCC
    AAGATCATGC AAACTGGAGG TGGATATGTA AACTGGGCAT TTTCAGAAGG TGATGAAACT
    GGTGTGTTTA GCATCAAGAA AAAGTGGCAA ACCTGCTTGC CCTCCACTTG TGACAGTGAT
    TCCTCTCGGA GTGAACAGCA CCAGAAGCAG GCCCAGGACA GCTCCCTATC TGATAACTCA
    ACAAGATCGG CCCAGAGTAG TGAATGCTCA GAGGTGGGAC CATGGCTTCA GCCAAACACA
    TCCTTTTGGA TCAATCCTCT CCGCAGATAC AGGCCCTTCG CTAGGAGTCA TAGTTTTAGA
    TTCCATAAGG AGGAGAAATT GATGAAGATC TGTAAGATTA AAAATCTTTC AGGCTCTTCA
    GAAATAGGGC AGGGAGCATG GGTCAAAGCG AAAATGCTAA CCAAAGACAG GAGACTGTCA
    AAGAAAAAGA AGAATACTCA AGGACTCCAG GTGCCAATCA TAACAGTCAA TGCCTGCTCT
    CAGAGTGACC AGTTGAATCC AGAGCCAGGA GAAAACAGCA TCTCTGAAGA GGAGTACAGC
    AAGAACTGGT TCACAGTGTC CAAATTTAGT CACACAGGTG TAGAACCTTA CATACATCAG
    AAAATGAAAA CTAAAGAAAT TGGACAATGT GCTATACAAA TCAGTGATTA CCTAAAGCAG
    TCTCAAGAGG ATCTCAGCAA AAACTCTTTG TGGAATTCCA GGAGCACCAA CCTCAATAGG
    AACTCCCTGC TGAAAAGTTC AATTGGAGTT GACAAGATCT CAGCCTCCTT AAAAAGCCCT
    CAAGAGCCTC ACCATCATTA TTCAGCCATT GAAAGGAATA ATTTAATGAG GCTTTCTCAG
    ACCATACCAT TTACACCAGT CCAACTGTTT GCAGGAGAAG AAATAACTGT CTACAGGTTG
    GAGGAGAGTT CCCCTTTAAA CCTTGATAAA AGCATGTCCT CTTGGTCTCA GCGTGGGAGA
    GCGGCAATGA TCCAGGTATT GTCCCGAGAG GAGATGGATG GGGGCCTCCG TAAAGCTATG
    AGAGTCGTCA GCACTTGGTC TGAGGATGAC ATTCTCAAGC CGGGACAAGT TTTCATTGTC
    AAGTCCTTTC TTCCTGAGGT TGTGCGGACA TGGCATAAAA TCTTCCAGGA GAGCACTGTG
    CTTCATCTTT GCCTCAGGGA AATTCAACAA CAAAGAGCTG CTCAAAAATT GATCTATACC
    TTCAACCAAG TGAAACCACA AACCATACCC TACACACCAA GGTTCCTGGA AGTTTTCTTA
    ATCTACTGCC ATTCAGCCAA CCAGTGGTTG ACCATTGAGA AGTATATGAC AGGGGAGTTC
    CGGAAGTATA ACAACAACAA TGGTGATGAA ATCACCCCCA CCAACACCCT GGAGGAGCTG
    ATGTTGGCTT TCTCTCACTG GACCTATGAG TACACTCGGG GAGAGCTGCT GGTTTTAGAT
    TTGCAAGGTG TTGGAGAAAA TTTGACAGAT CCATCTGTTA TAAAACCTGA AGTCAAACAA
    TCAAGAGGAA TGGTGTTTGG ACCGGCCAAT TTGGGGGAAG ATGCAATTAG AAACTTCATT
    GCAAAACATC ATTGTAACTC CTGCTGCCGG AAGCTCAAAC TCCCGGATTT AAAAAGAAAT
    GACTATTCCC CTGAAAGGAT AAATTCCACC TTTGGACTTG AGATAAAAAT AGAATCAGCT
    GAGGAGCCTC CAGCAAGGGA GACGGGTAGA AATTCCCCAG AAGATGATAT GCAACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75943
    Clone ID Related Accession (Same CDS sequence) XM_011518248.1
    Accession Version XM_011518248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAC
    CAGAGACACT CCTCAGGAAA TAGAAATGAG TCTGCAGAAA GTACGCTGCA CTCCCAGTTC
    ATTAGAACTG CACAGCCATA CAAATTCAAG GAAAAGTCTA TAGTCCTTCA TAAATCAAGG
    AAGAAGTCAA AAGAACAAAA TGTATCAGAT GACCCTGAGT CTACTGGCTT TCTTTACCCT
    TACAATGACC TGCTGGTTTG GGCTGTGCTG ATGAAAAGGC AGAAGATGGC TATGTTCTTC
    TGGCAGCATG GAGAGGAGGC CACGGTTAAA GCCGTGATTG CGTGTATCCT CTACCGGGCA
    ATGGCCCATG AAGCTAAGGA GAGTCACATG GTGGATGATG CCTCAGAAGA GTTGAAGAAT
    TACTCAAAAC AGTTTGGCCA GCTGGCTCTG GACTTGTTGG AGAAGGCATT CAAGCAGAAT
    GAGCGCATGG CCATGACGCT GTTGACGTAT GAACTCAGGA ACTGGAGCAA TTCGACCTGC
    CTTAAACTGG CCGTGTCGGG AGGATTACGA CCCTTTGTTT CACATACTTG TACCCAGATG
    CTACTGACAG ACATGTGGAT GGGGAGGCTG AAAATGAGGA AAAACTCTTG GTTAAAGATT
    ATTATAAGCA TTATTTTACC ACCCACCATT TTGACACTGG AATTTAAAAG CAAAGCTGAG
    ATGTCACATG TTCCCCAGTC CCAGGACTTC CAATTTATGT GGTATTACAG TGACCAGAAC
    GCCAGCAGTT CCAAAGAAAG TGCTTCTGTG AAAGAGTATG ATTTGGAAAG GGGCCATGAT
    GAGAAACTGG ATGAAAATCA GCATTTTGGT TTGGAAAGTG GGCACCAACA CCTTCCGTGG
    ACCAGGAAAG TCTATGAGTT CTACAGTGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GCGTATTTGG CATTCCTCAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AGGAGTGGCT TGTTAGCATT TACATCTTCA CCAATGCTAT TGAGGTGGTC
    AGGGAGATCT GTATTTCAGA ACCTGGGAAG TTTACCCAAA AGGTGAAGGT ATGGATTAGT
    GAGTACTGGA ACTTAACAGA AACTGTGGCC ATTGGCCTGT TTTCAGCTGG CTTCGTCCTT
    CGATGGGGTG ACCCTCCTTT TCACACAGCG GGAAGACTGA TCTACTGCAT AGACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCATATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTATATTG TGATCATCAT GGCCATAGTC
    CTGCTGAGCT TTGGAGTGGC ACGCAAGGCC ATCCTTTCGC CAAAAGAGCC ACCATCTTGG
    AGTCTAGCTC GAGATATTGT ATTTGAGCCA TACTGGATGA TATACGGAGA AGTCTATGCT
    GGAGAAATAG ATGTTTGTTC AAGCCAGCCA TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCTTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTCAACA ACGTTTACTT AGATATGGAA TCCATTTCAA ATAACCTGTG GAAATACAAC
    CGCTATCGCT ACATCATGAC CTACCACGAG AAGCCCTGGC TGCCCCCACC TCTCATCCTG
    CTGAGCCACG TGGGCCTTCT CCTCCGCCGC CTGTGCTGTC ATCGAGCTCC TCACGACCAA
    GAAGAGGGTG ACGTTGGATT AAAACTCTAC CTCAGTAAGG AGGATCTGAA AAAACTTCAT
    GATTTTGAGG AGCAGTGCGT GGAAAAATAC TTCCATGAGA AGATGGAAGA TGTGAATTGT
    AGTTGTGAGG AACGAATCCG AGTGACATCA GAAAGGGTTA CAGAGATGTA CTTCCAGCTG
    AAAGAAATGA ATGAAAAGGT GTCTTTTATA AAGGACTCCT TACTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGCCCTG ACTGTGGATA CCCTGAAAGT CCTTTCTGCT
    GTTGACACTT TGCAAGAGGA TGAGGCTCTC CTGGCCAAGA GAAAGCATTC TACTTGCAAA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTAGGCAG CATGGAGATC
    GCTGGAGAGA AGAAATACCA GTATTATAGC ATGCCCTCTT CTTTGCTGAG GAGCCTGGCT
    GGAGGCCGGC ATCCCCCAAG AGTGCAGAGG GGGGCACTTC TTGAGATTAC AAACAGTAAA
    AGAGAGGCTA CAAATGTAAG AAATGACCAG GAAAGGCAAG AAACACAAAG TAGTATAGTG
    GTTTCTGGGG TGTCTCCTAA CAGGCAAGCA CACTCAAAGT ATGGCCAGTT TCTTCTGGTC
    CCCTCTAATC TAAAGCGAGT TCCTTTTTCA GCAGAAACTG TCTTGCCTCT GTCCAGACCC
    TCTGTGCCAG ATGTGCTGGC AACTGAACAG GACATCCAGA CTGAGGTTCT TGTTCATCTG
    ACTGGGCAGA CCCCAGTTGT CTCTGACTGG GCATCAGTGG ATGAACCCAA GGAAAAGCAC
    GAGCCTATTG CTCACTTACT GGATGGACAA GACAAGGCAG AGCAAGTGCT ACCCACTTTG
    AGTTGCACAC CTGAACCCAT GACAATGAGC TCCCCTCTTT CCCAAGCCAA GATCATGCAA
    ACTGGAGGTG GATATGTAAA CTGGGCATTT TCAGAAGGTG ATGAAACTGG TGTGTTTAGC
    ATCAAGAAAA AGTGGCAAAC CTGCTTGCCC TCCACTTGTG ACAGTGATTC CTCTCGGAGT
    GAACAGCACC AGAAGCAGGC CCAGGACAGC TCCCTATCTG ATAACTCAAC AAGATCGGCC
    CAGAGTAGTG AATGCTCAGA GGTGGGACCA TGGCTTCAGC CAAACACATC CTTTTGGATC
    AATCCTCTCC GCAGATACAG GCCCTTCGCT AGGAGTCATA GTTTTAGATT CCATAAGGAG
    GAGAAATTGA TGAAGATCTG TAAGATTAAA AATCTTTCAG GCTCTTCAGA AATAGGGCAG
    GGAGCATGGG TCAAAGCGAA AATGCTAACC AAAGACAGGA GACTGTCAAA GAAAAAGAAG
    AATACTCAAG GACTCCAGGT GCCAATCATA ACAGTCAATG CCTGCTCTCA GAGTGACCAG
    TTGAATCCAG AGCCAGGAGA AAACAGCATC TCTGAAGAGG AGTACAGCAA GAACTGGTTC
    ACAGTGTCCA AATTTAGTCA CACAGGTGTA GAACCTTACA TACATCAGAA AATGAAAACT
    AAAGAAATTG GACAATGTGC TATACAAATC AGTGATTACC TAAAGCAGTC TCAAGAGGAT
    CTCAGCAAAA ACTCTTTGTG GAATTCCAGG AGCACCAACC TCAATAGGAA CTCCCTGCTG
    AAAAGTTCAA TTGGAGTTGA CAAGATCTCA GCCTCCTTAA AAAGCCCTCA AGAGCCTCAC
    CATCATTATT CAGCCATTGA AAGGAATAAT TTAATGAGGC TTTCTCAGAC CATACCATTT
    ACACCAGTCC AACTGTTTGC AGGAGAAGAA ATAACTGTCT ACAGGTTGGA GGAGAGTTCC
    CCTTTAAACC TTGATAAAAG CATGTCCTCT TGGTCTCAGC GTGGGAGAGC GGCAATGATC
    CAGGTATTGT CCCGAGAGGA GATGGATGGG GGCCTCCGTA AAGCTATGAG AGTCGTCAGC
    ACTTGGTCTG AGGATGACAT TCTCAAGCCG GGACAAGTTT TCATTGTCAA GTCCTTTCTT
    CCTGAGGTTG TGCGGACATG GCATAAAATC TTCCAGGAGA GCACTGTGCT TCATCTTTGC
    CTCAGGGAAA TTCAACAACA AAGAGCTGCT CAAAAATTGA TCTATACCTT CAACCAAGTG
    AAACCACAAA CCATACCCTA CACACCAAGG TTCCTGGAAG TTTTCTTAAT CTACTGCCAT
    TCAGCCAACC AGTGGTTGAC CATTGAGAAG TATATGACAG GGGAGTTCCG GAAGTATAAC
    AACAACAATG GTGATGAAAT CACCCCCACC AACACCCTGG AGGAGCTGAT GTTGGCTTTC
    TCTCACTGGA CCTATGAGTA CACTCGGGGA GAGCTGCTGG TTTTAGATTT GCAAGGTGTT
    GGAGAAAATT TGACAGATCC ATCTGTTATA AAACCTGAAG TCAAACAATC AAGAGGAATG
    GTGTTTGGAC CGGCCAATTT GGGGGAAGAT GCAATTAGAA ACTTCATTGC AAAACATCAT
    TGTAACTCCT GCTGCCGGAA GCTCAAACTC CCGGATTTAA AAAGAAATGA CTATTCCCCT
    GAAAGGATAA ATTCCACCTT TGGACTTGAG ATAAAAATAG AATCAGCTGA GGAGCCTCCA
    GCAAGGGAGA CGGGTAGAAA TTCCCCAGAA GATGATATGC AACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516550.1
    CDS239..6166
    Translation

    Target ORF information:

    RefSeq Version XM_011518248.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAC
    CAGAGACACT CCTCAGGAAA TAGAAATGAG TCTGCAGAAA GTACGCTGCA CTCCCAGTTC
    ATTAGAACTG CACAGCCATA CAAATTCAAG GAAAAGTCTA TAGTCCTTCA TAAATCAAGG
    AAGAAGTCAA AAGAACAAAA TGTATCAGAT GACCCTGAGT CTACTGGCTT TCTTTACCCT
    TACAATGACC TGCTGGTTTG GGCTGTGCTG ATGAAAAGGC AGAAGATGGC TATGTTCTTC
    TGGCAGCATG GAGAGGAGGC CACGGTTAAA GCCGTGATTG CGTGTATCCT CTACCGGGCA
    ATGGCCCATG AAGCTAAGGA GAGTCACATG GTGGATGATG CCTCAGAAGA GTTGAAGAAT
    TACTCAAAAC AGTTTGGCCA GCTGGCTCTG GACTTGTTGG AGAAGGCATT CAAGCAGAAT
    GAGCGCATGG CCATGACGCT GTTGACGTAT GAACTCAGGA ACTGGAGCAA TTCGACCTGC
    CTTAAACTGG CCGTGTCGGG AGGATTACGA CCCTTTGTTT CACATACTTG TACCCAGATG
    CTACTGACAG ACATGTGGAT GGGGAGGCTG AAAATGAGGA AAAACTCTTG GTTAAAGATT
    ATTATAAGCA TTATTTTACC ACCCACCATT TTGACACTGG AATTTAAAAG CAAAGCTGAG
    ATGTCACATG TTCCCCAGTC CCAGGACTTC CAATTTATGT GGTATTACAG TGACCAGAAC
    GCCAGCAGTT CCAAAGAAAG TGCTTCTGTG AAAGAGTATG ATTTGGAAAG GGGCCATGAT
    GAGAAACTGG ATGAAAATCA GCATTTTGGT TTGGAAAGTG GGCACCAACA CCTTCCGTGG
    ACCAGGAAAG TCTATGAGTT CTACAGTGCT CCAATTGTCA AGTTTTGGTT TTATACGATG
    GCGTATTTGG CATTCCTCAT GCTGTTCACT TACACCGTGT TGGTGGAGAT GCAGCCCCAG
    CCCAGCGTGC AGGAGTGGCT TGTTAGCATT TACATCTTCA CCAATGCTAT TGAGGTGGTC
    AGGGAGATCT GTATTTCAGA ACCTGGGAAG TTTACCCAAA AGGTGAAGGT ATGGATTAGT
    GAGTACTGGA ACTTAACAGA AACTGTGGCC ATTGGCCTGT TTTCAGCTGG CTTCGTCCTT
    CGATGGGGTG ACCCTCCTTT TCACACAGCG GGAAGACTGA TCTACTGCAT AGACATCATA
    TTCTGGTTCT CACGGCTCCT GGACTTCTTT GCTGTGAATC AACATGCAGG TCCATATGTG
    ACCATGATTG CAAAAATGAC AGCAAACATG TTCTATATTG TGATCATCAT GGCCATAGTC
    CTGCTGAGCT TTGGAGTGGC ACGCAAGGCC ATCCTTTCGC CAAAAGAGCC ACCATCTTGG
    AGTCTAGCTC GAGATATTGT ATTTGAGCCA TACTGGATGA TATACGGAGA AGTCTATGCT
    GGAGAAATAG ATGTTTGTTC AAGCCAGCCA TCCTGCCCTC CTGGTTCTTT TCTTACTCCA
    TTCTTGCAAG CTGTCTACCT CTTCGTGCAA TATATCATCA TGGTGAACCT GTTGATTGCT
    TTCTTCAACA ACGTTTACTT AGATATGGAA TCCATTTCAA ATAACCTGTG GAAATACAAC
    CGCTATCGCT ACATCATGAC CTACCACGAG AAGCCCTGGC TGCCCCCACC TCTCATCCTG
    CTGAGCCACG TGGGCCTTCT CCTCCGCCGC CTGTGCTGTC ATCGAGCTCC TCACGACCAA
    GAAGAGGGTG ACGTTGGATT AAAACTCTAC CTCAGTAAGG AGGATCTGAA AAAACTTCAT
    GATTTTGAGG AGCAGTGCGT GGAAAAATAC TTCCATGAGA AGATGGAAGA TGTGAATTGT
    AGTTGTGAGG AACGAATCCG AGTGACATCA GAAAGGGTTA CAGAGATGTA CTTCCAGCTG
    AAAGAAATGA ATGAAAAGGT GTCTTTTATA AAGGACTCCT TACTGTCTTT GGACAGCCAG
    GTGGGACACC TGCAGGATCT CTCTGCCCTG ACTGTGGATA CCCTGAAAGT CCTTTCTGCT
    GTTGACACTT TGCAAGAGGA TGAGGCTCTC CTGGCCAAGA GAAAGCATTC TACTTGCAAA
    AAACTTCCCC ACAGCTGGAG CAATGTCATC TGTGCAGAGG TTCTAGGCAG CATGGAGATC
    GCTGGAGAGA AGAAATACCA GTATTATAGC ATGCCCTCTT CTTTGCTGAG GAGCCTGGCT
    GGAGGCCGGC ATCCCCCAAG AGTGCAGAGG GGGGCACTTC TTGAGATTAC AAACAGTAAA
    AGAGAGGCTA CAAATGTAAG AAATGACCAG GAAAGGCAAG AAACACAAAG TAGTATAGTG
    GTTTCTGGGG TGTCTCCTAA CAGGCAAGCA CACTCAAAGT ATGGCCAGTT TCTTCTGGTC
    CCCTCTAATC TAAAGCGAGT TCCTTTTTCA GCAGAAACTG TCTTGCCTCT GTCCAGACCC
    TCTGTGCCAG ATGTGCTGGC AACTGAACAG GACATCCAGA CTGAGGTTCT TGTTCATCTG
    ACTGGGCAGA CCCCAGTTGT CTCTGACTGG GCATCAGTGG ATGAACCCAA GGAAAAGCAC
    GAGCCTATTG CTCACTTACT GGATGGACAA GACAAGGCAG AGCAAGTGCT ACCCACTTTG
    AGTTGCACAC CTGAACCCAT GACAATGAGC TCCCCTCTTT CCCAAGCCAA GATCATGCAA
    ACTGGAGGTG GATATGTAAA CTGGGCATTT TCAGAAGGTG ATGAAACTGG TGTGTTTAGC
    ATCAAGAAAA AGTGGCAAAC CTGCTTGCCC TCCACTTGTG ACAGTGATTC CTCTCGGAGT
    GAACAGCACC AGAAGCAGGC CCAGGACAGC TCCCTATCTG ATAACTCAAC AAGATCGGCC
    CAGAGTAGTG AATGCTCAGA GGTGGGACCA TGGCTTCAGC CAAACACATC CTTTTGGATC
    AATCCTCTCC GCAGATACAG GCCCTTCGCT AGGAGTCATA GTTTTAGATT CCATAAGGAG
    GAGAAATTGA TGAAGATCTG TAAGATTAAA AATCTTTCAG GCTCTTCAGA AATAGGGCAG
    GGAGCATGGG TCAAAGCGAA AATGCTAACC AAAGACAGGA GACTGTCAAA GAAAAAGAAG
    AATACTCAAG GACTCCAGGT GCCAATCATA ACAGTCAATG CCTGCTCTCA GAGTGACCAG
    TTGAATCCAG AGCCAGGAGA AAACAGCATC TCTGAAGAGG AGTACAGCAA GAACTGGTTC
    ACAGTGTCCA AATTTAGTCA CACAGGTGTA GAACCTTACA TACATCAGAA AATGAAAACT
    AAAGAAATTG GACAATGTGC TATACAAATC AGTGATTACC TAAAGCAGTC TCAAGAGGAT
    CTCAGCAAAA ACTCTTTGTG GAATTCCAGG AGCACCAACC TCAATAGGAA CTCCCTGCTG
    AAAAGTTCAA TTGGAGTTGA CAAGATCTCA GCCTCCTTAA AAAGCCCTCA AGAGCCTCAC
    CATCATTATT CAGCCATTGA AAGGAATAAT TTAATGAGGC TTTCTCAGAC CATACCATTT
    ACACCAGTCC AACTGTTTGC AGGAGAAGAA ATAACTGTCT ACAGGTTGGA GGAGAGTTCC
    CCTTTAAACC TTGATAAAAG CATGTCCTCT TGGTCTCAGC GTGGGAGAGC GGCAATGATC
    CAGGTATTGT CCCGAGAGGA GATGGATGGG GGCCTCCGTA AAGCTATGAG AGTCGTCAGC
    ACTTGGTCTG AGGATGACAT TCTCAAGCCG GGACAAGTTT TCATTGTCAA GTCCTTTCTT
    CCTGAGGTTG TGCGGACATG GCATAAAATC TTCCAGGAGA GCACTGTGCT TCATCTTTGC
    CTCAGGGAAA TTCAACAACA AAGAGCTGCT CAAAAATTGA TCTATACCTT CAACCAAGTG
    AAACCACAAA CCATACCCTA CACACCAAGG TTCCTGGAAG TTTTCTTAAT CTACTGCCAT
    TCAGCCAACC AGTGGTTGAC CATTGAGAAG TATATGACAG GGGAGTTCCG GAAGTATAAC
    AACAACAATG GTGATGAAAT CACCCCCACC AACACCCTGG AGGAGCTGAT GTTGGCTTTC
    TCTCACTGGA CCTATGAGTA CACTCGGGGA GAGCTGCTGG TTTTAGATTT GCAAGGTGTT
    GGAGAAAATT TGACAGATCC ATCTGTTATA AAACCTGAAG TCAAACAATC AAGAGGAATG
    GTGTTTGGAC CGGCCAATTT GGGGGAAGAT GCAATTAGAA ACTTCATTGC AAAACATCAT
    TGTAACTCCT GCTGCCGGAA GCTCAAACTC CCGGATTTAA AAAGAAATGA CTATTCCCCT
    GAAAGGATAA ATTCCACCTT TGGACTTGAG ATAAAAATAG AATCAGCTGA GGAGCCTCCA
    GCAAGGGAGA CGGGTAGAAA TTCCCCAGAA GATGATATGC AACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75944
    Clone ID Related Accession (Same CDS sequence) XM_011518249.1
    Accession Version XM_011518249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA
    AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC
    CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG
    AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC
    GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG
    GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC
    TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA
    CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC
    TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA
    ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG
    ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA
    TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA
    GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG
    GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA
    ATTGTCAAGT TTTGGTTTTA TACGATGGCG TATTTGGCAT TCCTCATGCT GTTCACTTAC
    ACCGTGTTGG TGGAGATGCA GCCCCAGCCC AGCGTGCAGG AGTGGCTTGT TAGCATTTAC
    ATCTTCACCA ATGCTATTGA GGTGGTCAGG GAGATCTGTA TTTCAGAACC TGGGAAGTTT
    ACCCAAAAGG TGAAGGTATG GATTAGTGAG TACTGGAACT TAACAGAAAC TGTGGCCATT
    GGCCTGTTTT CAGCTGGCTT CGTCCTTCGA TGGGGTGACC CTCCTTTTCA CACAGCGGGA
    AGACTGATCT ACTGCATAGA CATCATATTC TGGTTCTCAC GGCTCCTGGA CTTCTTTGCT
    GTGAATCAAC ATGCAGGTCC ATATGTGACC ATGATTGCAA AAATGACAGC AAACATGTTC
    TATATTGTGA TCATCATGGC CATAGTCCTG CTGAGCTTTG GAGTGGCACG CAAGGCCATC
    CTTTCGCCAA AAGAGCCACC ATCTTGGAGT CTAGCTCGAG ATATTGTATT TGAGCCATAC
    TGGATGATAT ACGGAGAAGT CTATGCTGGA GAAATAGATG TTTGTTCAAG CCAGCCATCC
    TGCCCTCCTG GTTCTTTTCT TACTCCATTC TTGCAAGCTG TCTACCTCTT CGTGCAATAT
    ATCATCATGG TGAACCTGTT GATTGCTTTC TTCAACAACG TTTACTTAGA TATGGAATCC
    ATTTCAAATA ACCTGTGGAA ATACAACCGC TATCGCTACA TCATGACCTA CCACGAGAAG
    CCCTGGCTGC CCCCACCTCT CATCCTGCTG AGCCACGTGG GCCTTCTCCT CCGCCGCCTG
    TGCTGTCATC GAGCTCCTCA CGACCAAGAA GAGGGTGACG TTGGATTAAA ACTCTACCTC
    AGTAAGGAGG ATCTGAAAAA ACTTCATGAT TTTGAGGAGC AGTGCGTGGA AAAATACTTC
    CATGAGAAGA TGGAAGATGT GAATTGTAGT TGTGAGGAAC GAATCCGAGT GACATCAGAA
    AGGGTTACAG AGATGTACTT CCAGCTGAAA GAAATGAATG AAAAGGTGTC TTTTATAAAG
    GACTCCTTAC TGTCTTTGGA CAGCCAGGTG GGACACCTGC AGGATCTCTC TGCCCTGACT
    GTGGATACCC TGAAAGTCCT TTCTGCTGTT GACACTTTGC AAGAGGATGA GGCTCTCCTG
    GCCAAGAGAA AGCATTCTAC TTGCAAAAAA CTTCCCCACA GCTGGAGCAA TGTCATCTGT
    GCAGAGGTTC TAGGCAGCAT GGAGATCGCT GGAGAGAAGA AATACCAGTA TTATAGCATG
    CCCTCTTCTT TGCTGAGGAG CCTGGCTGGA GGCCGGCATC CCCCAAGAGT GCAGAGGGGG
    GCACTTCTTG AGATTACAAA CAGTAAAAGA GAGGCTACAA ATGTAAGAAA TGACCAGGAA
    AGGCAAGAAA CACAAAGTAG TATAGTGGTT TCTGGGGTGT CTCCTAACAG GCAAGCACAC
    TCAAAGTATG GCCAGTTTCT TCTGGTCCCC TCTAATCTAA AGCGAGTTCC TTTTTCAGCA
    GAAACTGTCT TGCCTCTGTC CAGACCCTCT GTGCCAGATG TGCTGGCAAC TGAACAGGAC
    ATCCAGACTG AGGTTCTTGT TCATCTGACT GGGCAGACCC CAGTTGTCTC TGACTGGGCA
    TCAGTGGATG AACCCAAGGA AAAGCACGAG CCTATTGCTC ACTTACTGGA TGGACAAGAC
    AAGGCAGAGC AAGTGCTACC CACTTTGAGT TGCACACCTG AACCCATGAC AATGAGCTCC
    CCTCTTTCCC AAGCCAAGAT CATGCAAACT GGAGGTGGAT ATGTAAACTG GGCATTTTCA
    GAAGGTGATG AAACTGGTGT GTTTAGCATC AAGAAAAAGT GGCAAACCTG CTTGCCCTCC
    ACTTGTGACA GTGATTCCTC TCGGAGTGAA CAGCACCAGA AGCAGGCCCA GGACAGCTCC
    CTATCTGATA ACTCAACAAG ATCGGCCCAG AGTAGTGAAT GCTCAGAGGT GGGACCATGG
    CTTCAGCCAA ACACATCCTT TTGGATCAAT CCTCTCCGCA GATACAGGCC CTTCGCTAGG
    AGTCATAGTT TTAGATTCCA TAAGGAGGAG AAATTGATGA AGATCTGTAA GATTAAAAAT
    CTTTCAGGCT CTTCAGAAAT AGGGCAGGGA GCATGGGTCA AAGCGAAAAT GCTAACCAAA
    GACAGGAGAC TGTCAAAGAA AAAGAAGAAT ACTCAAGGAC TCCAGGTGCC AATCATAACA
    GTCAATGCCT GCTCTCAGAG TGACCAGTTG AATCCAGAGC CAGGAGAAAA CAGCATCTCT
    GAAGAGGAGT ACAGCAAGAA CTGGTTCACA GTGTCCAAAT TTAGTCACAC AGGTGTAGAA
    CCTTACATAC ATCAGAAAAT GAAAACTAAA GAAATTGGAC AATGTGCTAT ACAAATCAGT
    GATTACCTAA AGCAGTCTCA AGAGGATCTC AGCAAAAACT CTTTGTGGAA TTCCAGGAGC
    ACCAACCTCA ATAGGAACTC CCTGCTGAAA AGTTCAATTG GAGTTGACAA GATCTCAGCC
    TCCTTAAAAA GCCCTCAAGA GCCTCACCAT CATTATTCAG CCATTGAAAG GAATAATTTA
    ATGAGGCTTT CTCAGACCAT ACCATTTACA CCAGTCCAAC TGTTTGCAGG AGAAGAAATA
    ACTGTCTACA GGTTGGAGGA GAGTTCCCCT TTAAACCTTG ATAAAAGCAT GTCCTCTTGG
    TCTCAGCGTG GGAGAGCGGC AATGATCCAG GTATTGTCCC GAGAGGAGAT GGATGGGGGC
    CTCCGTAAAG CTATGAGAGT CGTCAGCACT TGGTCTGAGG ATGACATTCT CAAGCCGGGA
    CAAGTTTTCA TTGTCAAGTC CTTTCTTCCT GAGGTTGTGC GGACATGGCA TAAAATCTTC
    CAGGAGAGCA CTGTGCTTCA TCTTTGCCTC AGGGAAATTC AACAACAAAG AGCTGCTCAA
    AAATTGATCT ATACCTTCAA CCAAGTGAAA CCACAAACCA TACCCTACAC ACCAAGGTTC
    CTGGAAGTTT TCTTAATCTA CTGCCATTCA GCCAACCAGT GGTTGACCAT TGAGAAGTAT
    ATGACAGGGG AGTTCCGGAA GTATAACAAC AACAATGGTG ATGAAATCAC CCCCACCAAC
    ACCCTGGAGG AGCTGATGTT GGCTTTCTCT CACTGGACCT ATGAGTACAC TCGGGGAGAG
    CTGCTGGTTT TAGATTTGCA AGGTGTTGGA GAAAATTTGA CAGATCCATC TGTTATAAAA
    CCTGAAGTCA AACAATCAAG AGGAATGGTG TTTGGACCGG CCAATTTGGG GGAAGATGCA
    ATTAGAAACT TCATTGCAAA ACATCATTGT AACTCCTGCT GCCGGAAGCT CAAACTCCCG
    GATTTAAAAA GAAATGACTA TTCCCCTGAA AGGATAAATT CCACCTTTGG ACTTGAGATA
    AAAATAGAAT CAGCTGAGGA GCCTCCAGCA AGGGAGACGG GTAGAAATTC CCCAGAAGAT
    GATATGCAAC TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516551.1
    CDS239..6073
    Translation

    Target ORF information:

    RefSeq Version XM_011518249.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGGAA
    AAGTCTATAG TCCTTCATAA ATCAAGGAAG AAGTCAAAAG AACAAAATGT ATCAGATGAC
    CCTGAGTCTA CTGGCTTTCT TTACCCTTAC AATGACCTGC TGGTTTGGGC TGTGCTGATG
    AAAAGGCAGA AGATGGCTAT GTTCTTCTGG CAGCATGGAG AGGAGGCCAC GGTTAAAGCC
    GTGATTGCGT GTATCCTCTA CCGGGCAATG GCCCATGAAG CTAAGGAGAG TCACATGGTG
    GATGATGCCT CAGAAGAGTT GAAGAATTAC TCAAAACAGT TTGGCCAGCT GGCTCTGGAC
    TTGTTGGAGA AGGCATTCAA GCAGAATGAG CGCATGGCCA TGACGCTGTT GACGTATGAA
    CTCAGGAACT GGAGCAATTC GACCTGCCTT AAACTGGCCG TGTCGGGAGG ATTACGACCC
    TTTGTTTCAC ATACTTGTAC CCAGATGCTA CTGACAGACA TGTGGATGGG GAGGCTGAAA
    ATGAGGAAAA ACTCTTGGTT AAAGATTATT ATAAGCATTA TTTTACCACC CACCATTTTG
    ACACTGGAAT TTAAAAGCAA AGCTGAGATG TCACATGTTC CCCAGTCCCA GGACTTCCAA
    TTTATGTGGT ATTACAGTGA CCAGAACGCC AGCAGTTCCA AAGAAAGTGC TTCTGTGAAA
    GAGTATGATT TGGAAAGGGG CCATGATGAG AAACTGGATG AAAATCAGCA TTTTGGTTTG
    GAAAGTGGGC ACCAACACCT TCCGTGGACC AGGAAAGTCT ATGAGTTCTA CAGTGCTCCA
    ATTGTCAAGT TTTGGTTTTA TACGATGGCG TATTTGGCAT TCCTCATGCT GTTCACTTAC
    ACCGTGTTGG TGGAGATGCA GCCCCAGCCC AGCGTGCAGG AGTGGCTTGT TAGCATTTAC
    ATCTTCACCA ATGCTATTGA GGTGGTCAGG GAGATCTGTA TTTCAGAACC TGGGAAGTTT
    ACCCAAAAGG TGAAGGTATG GATTAGTGAG TACTGGAACT TAACAGAAAC TGTGGCCATT
    GGCCTGTTTT CAGCTGGCTT CGTCCTTCGA TGGGGTGACC CTCCTTTTCA CACAGCGGGA
    AGACTGATCT ACTGCATAGA CATCATATTC TGGTTCTCAC GGCTCCTGGA CTTCTTTGCT
    GTGAATCAAC ATGCAGGTCC ATATGTGACC ATGATTGCAA AAATGACAGC AAACATGTTC
    TATATTGTGA TCATCATGGC CATAGTCCTG CTGAGCTTTG GAGTGGCACG CAAGGCCATC
    CTTTCGCCAA AAGAGCCACC ATCTTGGAGT CTAGCTCGAG ATATTGTATT TGAGCCATAC
    TGGATGATAT ACGGAGAAGT CTATGCTGGA GAAATAGATG TTTGTTCAAG CCAGCCATCC
    TGCCCTCCTG GTTCTTTTCT TACTCCATTC TTGCAAGCTG TCTACCTCTT CGTGCAATAT
    ATCATCATGG TGAACCTGTT GATTGCTTTC TTCAACAACG TTTACTTAGA TATGGAATCC
    ATTTCAAATA ACCTGTGGAA ATACAACCGC TATCGCTACA TCATGACCTA CCACGAGAAG
    CCCTGGCTGC CCCCACCTCT CATCCTGCTG AGCCACGTGG GCCTTCTCCT CCGCCGCCTG
    TGCTGTCATC GAGCTCCTCA CGACCAAGAA GAGGGTGACG TTGGATTAAA ACTCTACCTC
    AGTAAGGAGG ATCTGAAAAA ACTTCATGAT TTTGAGGAGC AGTGCGTGGA AAAATACTTC
    CATGAGAAGA TGGAAGATGT GAATTGTAGT TGTGAGGAAC GAATCCGAGT GACATCAGAA
    AGGGTTACAG AGATGTACTT CCAGCTGAAA GAAATGAATG AAAAGGTGTC TTTTATAAAG
    GACTCCTTAC TGTCTTTGGA CAGCCAGGTG GGACACCTGC AGGATCTCTC TGCCCTGACT
    GTGGATACCC TGAAAGTCCT TTCTGCTGTT GACACTTTGC AAGAGGATGA GGCTCTCCTG
    GCCAAGAGAA AGCATTCTAC TTGCAAAAAA CTTCCCCACA GCTGGAGCAA TGTCATCTGT
    GCAGAGGTTC TAGGCAGCAT GGAGATCGCT GGAGAGAAGA AATACCAGTA TTATAGCATG
    CCCTCTTCTT TGCTGAGGAG CCTGGCTGGA GGCCGGCATC CCCCAAGAGT GCAGAGGGGG
    GCACTTCTTG AGATTACAAA CAGTAAAAGA GAGGCTACAA ATGTAAGAAA TGACCAGGAA
    AGGCAAGAAA CACAAAGTAG TATAGTGGTT TCTGGGGTGT CTCCTAACAG GCAAGCACAC
    TCAAAGTATG GCCAGTTTCT TCTGGTCCCC TCTAATCTAA AGCGAGTTCC TTTTTCAGCA
    GAAACTGTCT TGCCTCTGTC CAGACCCTCT GTGCCAGATG TGCTGGCAAC TGAACAGGAC
    ATCCAGACTG AGGTTCTTGT TCATCTGACT GGGCAGACCC CAGTTGTCTC TGACTGGGCA
    TCAGTGGATG AACCCAAGGA AAAGCACGAG CCTATTGCTC ACTTACTGGA TGGACAAGAC
    AAGGCAGAGC AAGTGCTACC CACTTTGAGT TGCACACCTG AACCCATGAC AATGAGCTCC
    CCTCTTTCCC AAGCCAAGAT CATGCAAACT GGAGGTGGAT ATGTAAACTG GGCATTTTCA
    GAAGGTGATG AAACTGGTGT GTTTAGCATC AAGAAAAAGT GGCAAACCTG CTTGCCCTCC
    ACTTGTGACA GTGATTCCTC TCGGAGTGAA CAGCACCAGA AGCAGGCCCA GGACAGCTCC
    CTATCTGATA ACTCAACAAG ATCGGCCCAG AGTAGTGAAT GCTCAGAGGT GGGACCATGG
    CTTCAGCCAA ACACATCCTT TTGGATCAAT CCTCTCCGCA GATACAGGCC CTTCGCTAGG
    AGTCATAGTT TTAGATTCCA TAAGGAGGAG AAATTGATGA AGATCTGTAA GATTAAAAAT
    CTTTCAGGCT CTTCAGAAAT AGGGCAGGGA GCATGGGTCA AAGCGAAAAT GCTAACCAAA
    GACAGGAGAC TGTCAAAGAA AAAGAAGAAT ACTCAAGGAC TCCAGGTGCC AATCATAACA
    GTCAATGCCT GCTCTCAGAG TGACCAGTTG AATCCAGAGC CAGGAGAAAA CAGCATCTCT
    GAAGAGGAGT ACAGCAAGAA CTGGTTCACA GTGTCCAAAT TTAGTCACAC AGGTGTAGAA
    CCTTACATAC ATCAGAAAAT GAAAACTAAA GAAATTGGAC AATGTGCTAT ACAAATCAGT
    GATTACCTAA AGCAGTCTCA AGAGGATCTC AGCAAAAACT CTTTGTGGAA TTCCAGGAGC
    ACCAACCTCA ATAGGAACTC CCTGCTGAAA AGTTCAATTG GAGTTGACAA GATCTCAGCC
    TCCTTAAAAA GCCCTCAAGA GCCTCACCAT CATTATTCAG CCATTGAAAG GAATAATTTA
    ATGAGGCTTT CTCAGACCAT ACCATTTACA CCAGTCCAAC TGTTTGCAGG AGAAGAAATA
    ACTGTCTACA GGTTGGAGGA GAGTTCCCCT TTAAACCTTG ATAAAAGCAT GTCCTCTTGG
    TCTCAGCGTG GGAGAGCGGC AATGATCCAG GTATTGTCCC GAGAGGAGAT GGATGGGGGC
    CTCCGTAAAG CTATGAGAGT CGTCAGCACT TGGTCTGAGG ATGACATTCT CAAGCCGGGA
    CAAGTTTTCA TTGTCAAGTC CTTTCTTCCT GAGGTTGTGC GGACATGGCA TAAAATCTTC
    CAGGAGAGCA CTGTGCTTCA TCTTTGCCTC AGGGAAATTC AACAACAAAG AGCTGCTCAA
    AAATTGATCT ATACCTTCAA CCAAGTGAAA CCACAAACCA TACCCTACAC ACCAAGGTTC
    CTGGAAGTTT TCTTAATCTA CTGCCATTCA GCCAACCAGT GGTTGACCAT TGAGAAGTAT
    ATGACAGGGG AGTTCCGGAA GTATAACAAC AACAATGGTG ATGAAATCAC CCCCACCAAC
    ACCCTGGAGG AGCTGATGTT GGCTTTCTCT CACTGGACCT ATGAGTACAC TCGGGGAGAG
    CTGCTGGTTT TAGATTTGCA AGGTGTTGGA GAAAATTTGA CAGATCCATC TGTTATAAAA
    CCTGAAGTCA AACAATCAAG AGGAATGGTG TTTGGACCGG CCAATTTGGG GGAAGATGCA
    ATTAGAAACT TCATTGCAAA ACATCATTGT AACTCCTGCT GCCGGAAGCT CAAACTCCCG
    GATTTAAAAA GAAATGACTA TTCCCCTGAA AGGATAAATT CCACCTTTGG ACTTGAGATA
    AAAATAGAAT CAGCTGAGGA GCCTCCAGCA AGGGAGACGG GTAGAAATTC CCCAGAAGAT
    GATATGCAAC TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75945
    Clone ID Related Accession (Same CDS sequence) XM_011518250.1
    Accession Version XM_011518250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GATCTGTATT
    TCAGAACCTG GGAAGTTTAC CCAAAAGGTG AAGGTATGGA TTAGTGAGTA CTGGAACTTA
    ACAGAAACTG TGGCCATTGG CCTGTTTTCA GCTGGCTTCG TCCTTCGATG GGGTGACCCT
    CCTTTTCACA CAGCGGGAAG ACTGATCTAC TGCATAGACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCAT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA TATTGTGATC ATCATGGCCA TAGTCCTGCT GAGCTTTGGA
    GTGGCACGCA AGGCCATCCT TTCGCCAAAA GAGCCACCAT CTTGGAGTCT AGCTCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAC GGAGAAGTCT ATGCTGGAGA AATAGATGTT
    TGTTCAAGCC AGCCATCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCTT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT CAACAACGTT
    TACTTAGATA TGGAATCCAT TTCAAATAAC CTGTGGAAAT ACAACCGCTA TCGCTACATC
    ATGACCTACC ACGAGAAGCC CTGGCTGCCC CCACCTCTCA TCCTGCTGAG CCACGTGGGC
    CTTCTCCTCC GCCGCCTGTG CTGTCATCGA GCTCCTCACG ACCAAGAAGA GGGTGACGTT
    GGATTAAAAC TCTACCTCAG TAAGGAGGAT CTGAAAAAAC TTCATGATTT TGAGGAGCAG
    TGCGTGGAAA AATACTTCCA TGAGAAGATG GAAGATGTGA ATTGTAGTTG TGAGGAACGA
    ATCCGAGTGA CATCAGAAAG GGTTACAGAG ATGTACTTCC AGCTGAAAGA AATGAATGAA
    AAGGTGTCTT TTATAAAGGA CTCCTTACTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG CCCTGACTGT GGATACCCTG AAAGTCCTTT CTGCTGTTGA CACTTTGCAA
    GAGGATGAGG CTCTCCTGGC CAAGAGAAAG CATTCTACTT GCAAAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTA GGCAGCATGG AGATCGCTGG AGAGAAGAAA
    TACCAGTATT ATAGCATGCC CTCTTCTTTG CTGAGGAGCC TGGCTGGAGG CCGGCATCCC
    CCAAGAGTGC AGAGGGGGGC ACTTCTTGAG ATTACAAACA GTAAAAGAGA GGCTACAAAT
    GTAAGAAATG ACCAGGAAAG GCAAGAAACA CAAAGTAGTA TAGTGGTTTC TGGGGTGTCT
    CCTAACAGGC AAGCACACTC AAAGTATGGC CAGTTTCTTC TGGTCCCCTC TAATCTAAAG
    CGAGTTCCTT TTTCAGCAGA AACTGTCTTG CCTCTGTCCA GACCCTCTGT GCCAGATGTG
    CTGGCAACTG AACAGGACAT CCAGACTGAG GTTCTTGTTC ATCTGACTGG GCAGACCCCA
    GTTGTCTCTG ACTGGGCATC AGTGGATGAA CCCAAGGAAA AGCACGAGCC TATTGCTCAC
    TTACTGGATG GACAAGACAA GGCAGAGCAA GTGCTACCCA CTTTGAGTTG CACACCTGAA
    CCCATGACAA TGAGCTCCCC TCTTTCCCAA GCCAAGATCA TGCAAACTGG AGGTGGATAT
    GTAAACTGGG CATTTTCAGA AGGTGATGAA ACTGGTGTGT TTAGCATCAA GAAAAAGTGG
    CAAACCTGCT TGCCCTCCAC TTGTGACAGT GATTCCTCTC GGAGTGAACA GCACCAGAAG
    CAGGCCCAGG ACAGCTCCCT ATCTGATAAC TCAACAAGAT CGGCCCAGAG TAGTGAATGC
    TCAGAGGTGG GACCATGGCT TCAGCCAAAC ACATCCTTTT GGATCAATCC TCTCCGCAGA
    TACAGGCCCT TCGCTAGGAG TCATAGTTTT AGATTCCATA AGGAGGAGAA ATTGATGAAG
    ATCTGTAAGA TTAAAAATCT TTCAGGCTCT TCAGAAATAG GGCAGGGAGC ATGGGTCAAA
    GCGAAAATGC TAACCAAAGA CAGGAGACTG TCAAAGAAAA AGAAGAATAC TCAAGGACTC
    CAGGTGCCAA TCATAACAGT CAATGCCTGC TCTCAGAGTG ACCAGTTGAA TCCAGAGCCA
    GGAGAAAACA GCATCTCTGA AGAGGAGTAC AGCAAGAACT GGTTCACAGT GTCCAAATTT
    AGTCACACAG GTGTAGAACC TTACATACAT CAGAAAATGA AAACTAAAGA AATTGGACAA
    TGTGCTATAC AAATCAGTGA TTACCTAAAG CAGTCTCAAG AGGATCTCAG CAAAAACTCT
    TTGTGGAATT CCAGGAGCAC CAACCTCAAT AGGAACTCCC TGCTGAAAAG TTCAATTGGA
    GTTGACAAGA TCTCAGCCTC CTTAAAAAGC CCTCAAGAGC CTCACCATCA TTATTCAGCC
    ATTGAAAGGA ATAATTTAAT GAGGCTTTCT CAGACCATAC CATTTACACC AGTCCAACTG
    TTTGCAGGAG AAGAAATAAC TGTCTACAGG TTGGAGGAGA GTTCCCCTTT AAACCTTGAT
    AAAAGCATGT CCTCTTGGTC TCAGCGTGGG AGAGCGGCAA TGATCCAGGT ATTGTCCCGA
    GAGGAGATGG ATGGGGGCCT CCGTAAAGCT ATGAGAGTCG TCAGCACTTG GTCTGAGGAT
    GACATTCTCA AGCCGGGACA AGTTTTCATT GTCAAGTCCT TTCTTCCTGA GGTTGTGCGG
    ACATGGCATA AAATCTTCCA GGAGAGCACT GTGCTTCATC TTTGCCTCAG GGAAATTCAA
    CAACAAAGAG CTGCTCAAAA ATTGATCTAT ACCTTCAACC AAGTGAAACC ACAAACCATA
    CCCTACACAC CAAGGTTCCT GGAAGTTTTC TTAATCTACT GCCATTCAGC CAACCAGTGG
    TTGACCATTG AGAAGTATAT GACAGGGGAG TTCCGGAAGT ATAACAACAA CAATGGTGAT
    GAAATCACCC CCACCAACAC CCTGGAGGAG CTGATGTTGG CTTTCTCTCA CTGGACCTAT
    GAGTACACTC GGGGAGAGCT GCTGGTTTTA GATTTGCAAG GTGTTGGAGA AAATTTGACA
    GATCCATCTG TTATAAAACC TGAAGTCAAA CAATCAAGAG GAATGGTGTT TGGACCGGCC
    AATTTGGGGG AAGATGCAAT TAGAAACTTC ATTGCAAAAC ATCATTGTAA CTCCTGCTGC
    CGGAAGCTCA AACTCCCGGA TTTAAAAAGA AATGACTATT CCCCTGAAAG GATAAATTCC
    ACCTTTGGAC TTGAGATAAA AATAGAATCA GCTGAGGAGC CTCCAGCAAG GGAGACGGGT
    AGAAATTCCC CAGAAGATGA TATGCAACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516552.1
    CDS239..6031
    Translation

    Target ORF information:

    RefSeq Version XM_011518250.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GATCTGTATT
    TCAGAACCTG GGAAGTTTAC CCAAAAGGTG AAGGTATGGA TTAGTGAGTA CTGGAACTTA
    ACAGAAACTG TGGCCATTGG CCTGTTTTCA GCTGGCTTCG TCCTTCGATG GGGTGACCCT
    CCTTTTCACA CAGCGGGAAG ACTGATCTAC TGCATAGACA TCATATTCTG GTTCTCACGG
    CTCCTGGACT TCTTTGCTGT GAATCAACAT GCAGGTCCAT ATGTGACCAT GATTGCAAAA
    ATGACAGCAA ACATGTTCTA TATTGTGATC ATCATGGCCA TAGTCCTGCT GAGCTTTGGA
    GTGGCACGCA AGGCCATCCT TTCGCCAAAA GAGCCACCAT CTTGGAGTCT AGCTCGAGAT
    ATTGTATTTG AGCCATACTG GATGATATAC GGAGAAGTCT ATGCTGGAGA AATAGATGTT
    TGTTCAAGCC AGCCATCCTG CCCTCCTGGT TCTTTTCTTA CTCCATTCTT GCAAGCTGTC
    TACCTCTTCG TGCAATATAT CATCATGGTG AACCTGTTGA TTGCTTTCTT CAACAACGTT
    TACTTAGATA TGGAATCCAT TTCAAATAAC CTGTGGAAAT ACAACCGCTA TCGCTACATC
    ATGACCTACC ACGAGAAGCC CTGGCTGCCC CCACCTCTCA TCCTGCTGAG CCACGTGGGC
    CTTCTCCTCC GCCGCCTGTG CTGTCATCGA GCTCCTCACG ACCAAGAAGA GGGTGACGTT
    GGATTAAAAC TCTACCTCAG TAAGGAGGAT CTGAAAAAAC TTCATGATTT TGAGGAGCAG
    TGCGTGGAAA AATACTTCCA TGAGAAGATG GAAGATGTGA ATTGTAGTTG TGAGGAACGA
    ATCCGAGTGA CATCAGAAAG GGTTACAGAG ATGTACTTCC AGCTGAAAGA AATGAATGAA
    AAGGTGTCTT TTATAAAGGA CTCCTTACTG TCTTTGGACA GCCAGGTGGG ACACCTGCAG
    GATCTCTCTG CCCTGACTGT GGATACCCTG AAAGTCCTTT CTGCTGTTGA CACTTTGCAA
    GAGGATGAGG CTCTCCTGGC CAAGAGAAAG CATTCTACTT GCAAAAAACT TCCCCACAGC
    TGGAGCAATG TCATCTGTGC AGAGGTTCTA GGCAGCATGG AGATCGCTGG AGAGAAGAAA
    TACCAGTATT ATAGCATGCC CTCTTCTTTG CTGAGGAGCC TGGCTGGAGG CCGGCATCCC
    CCAAGAGTGC AGAGGGGGGC ACTTCTTGAG ATTACAAACA GTAAAAGAGA GGCTACAAAT
    GTAAGAAATG ACCAGGAAAG GCAAGAAACA CAAAGTAGTA TAGTGGTTTC TGGGGTGTCT
    CCTAACAGGC AAGCACACTC AAAGTATGGC CAGTTTCTTC TGGTCCCCTC TAATCTAAAG
    CGAGTTCCTT TTTCAGCAGA AACTGTCTTG CCTCTGTCCA GACCCTCTGT GCCAGATGTG
    CTGGCAACTG AACAGGACAT CCAGACTGAG GTTCTTGTTC ATCTGACTGG GCAGACCCCA
    GTTGTCTCTG ACTGGGCATC AGTGGATGAA CCCAAGGAAA AGCACGAGCC TATTGCTCAC
    TTACTGGATG GACAAGACAA GGCAGAGCAA GTGCTACCCA CTTTGAGTTG CACACCTGAA
    CCCATGACAA TGAGCTCCCC TCTTTCCCAA GCCAAGATCA TGCAAACTGG AGGTGGATAT
    GTAAACTGGG CATTTTCAGA AGGTGATGAA ACTGGTGTGT TTAGCATCAA GAAAAAGTGG
    CAAACCTGCT TGCCCTCCAC TTGTGACAGT GATTCCTCTC GGAGTGAACA GCACCAGAAG
    CAGGCCCAGG ACAGCTCCCT ATCTGATAAC TCAACAAGAT CGGCCCAGAG TAGTGAATGC
    TCAGAGGTGG GACCATGGCT TCAGCCAAAC ACATCCTTTT GGATCAATCC TCTCCGCAGA
    TACAGGCCCT TCGCTAGGAG TCATAGTTTT AGATTCCATA AGGAGGAGAA ATTGATGAAG
    ATCTGTAAGA TTAAAAATCT TTCAGGCTCT TCAGAAATAG GGCAGGGAGC ATGGGTCAAA
    GCGAAAATGC TAACCAAAGA CAGGAGACTG TCAAAGAAAA AGAAGAATAC TCAAGGACTC
    CAGGTGCCAA TCATAACAGT CAATGCCTGC TCTCAGAGTG ACCAGTTGAA TCCAGAGCCA
    GGAGAAAACA GCATCTCTGA AGAGGAGTAC AGCAAGAACT GGTTCACAGT GTCCAAATTT
    AGTCACACAG GTGTAGAACC TTACATACAT CAGAAAATGA AAACTAAAGA AATTGGACAA
    TGTGCTATAC AAATCAGTGA TTACCTAAAG CAGTCTCAAG AGGATCTCAG CAAAAACTCT
    TTGTGGAATT CCAGGAGCAC CAACCTCAAT AGGAACTCCC TGCTGAAAAG TTCAATTGGA
    GTTGACAAGA TCTCAGCCTC CTTAAAAAGC CCTCAAGAGC CTCACCATCA TTATTCAGCC
    ATTGAAAGGA ATAATTTAAT GAGGCTTTCT CAGACCATAC CATTTACACC AGTCCAACTG
    TTTGCAGGAG AAGAAATAAC TGTCTACAGG TTGGAGGAGA GTTCCCCTTT AAACCTTGAT
    AAAAGCATGT CCTCTTGGTC TCAGCGTGGG AGAGCGGCAA TGATCCAGGT ATTGTCCCGA
    GAGGAGATGG ATGGGGGCCT CCGTAAAGCT ATGAGAGTCG TCAGCACTTG GTCTGAGGAT
    GACATTCTCA AGCCGGGACA AGTTTTCATT GTCAAGTCCT TTCTTCCTGA GGTTGTGCGG
    ACATGGCATA AAATCTTCCA GGAGAGCACT GTGCTTCATC TTTGCCTCAG GGAAATTCAA
    CAACAAAGAG CTGCTCAAAA ATTGATCTAT ACCTTCAACC AAGTGAAACC ACAAACCATA
    CCCTACACAC CAAGGTTCCT GGAAGTTTTC TTAATCTACT GCCATTCAGC CAACCAGTGG
    TTGACCATTG AGAAGTATAT GACAGGGGAG TTCCGGAAGT ATAACAACAA CAATGGTGAT
    GAAATCACCC CCACCAACAC CCTGGAGGAG CTGATGTTGG CTTTCTCTCA CTGGACCTAT
    GAGTACACTC GGGGAGAGCT GCTGGTTTTA GATTTGCAAG GTGTTGGAGA AAATTTGACA
    GATCCATCTG TTATAAAACC TGAAGTCAAA CAATCAAGAG GAATGGTGTT TGGACCGGCC
    AATTTGGGGG AAGATGCAAT TAGAAACTTC ATTGCAAAAC ATCATTGTAA CTCCTGCTGC
    CGGAAGCTCA AACTCCCGGA TTTAAAAAGA AATGACTATT CCCCTGAAAG GATAAATTCC
    ACCTTTGGAC TTGAGATAAA AATAGAATCA GCTGAGGAGC CTCCAGCAAG GGAGACGGGT
    AGAAATTCCC CAGAAGATGA TATGCAACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75947
    Clone ID Related Accession (Same CDS sequence) XM_011518252.2
    Accession Version XM_011518252.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGAC CTTAAATGCT GAAGATATTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516554.1
    CDS239..5323
    Translation

    Target ORF information:

    RefSeq Version XM_011518252.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518252.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGAC CTTAAATGCT GAAGATATTA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22680
    Clone ID Related Accession (Same CDS sequence) NM_001177311.1
    Accession Version NM_001177311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY333284.1, AF350881.2 and AL354795.17. Transcript Variant: This variant (c) contains an alternate 5' terminal exon compared to variant a. This results in a shorter isoform (c) with a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY333284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGACAGCGC CGGTTACGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
    TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC
    TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT
    TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC
    ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT
    TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC
    ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA
    GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG
    CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG
    TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC
    TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC
    AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC
    GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG
    ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC
    CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG
    GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT
    TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC
    TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT
    TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG
    AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA
    GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT
    ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC
    TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT
    CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC
    AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC
    CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA
    TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT
    TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG
    TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC
    CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG
    AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG
    CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG
    ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC
    CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA
    GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC
    CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC
    CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT
    CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT
    ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG
    CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG
    GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG
    ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC
    GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC
    ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA
    TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC
    ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA
    TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC
    TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT
    ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG
    ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA
    TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC
    ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC
    GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA
    CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG
    AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC
    CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC
    AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT
    TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT
    TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG
    GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC
    CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC
    AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT
    ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT
    CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC
    AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT
    CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA
    AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC
    ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC
    ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG
    TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT
    CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA
    TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT
    TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT
    AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA
    GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA
    AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT
    GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC
    TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG
    AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA
    GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC
    CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG
    CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA
    CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG
    AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA
    ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC
    GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC
    TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT
    CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC
    CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC
    TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG
    TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG
    GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA
    GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA
    GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA
    CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT
    TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG
    CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001170782.1
    CDS100..6153
    Translation

    Target ORF information:

    RefSeq Version NM_001177311.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant c, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGACAGCGC CGGTTACGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
    TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC
    TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT
    TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC
    ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT
    TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC
    ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA
    GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG
    CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG
    TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC
    TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC
    AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC
    GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG
    ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC
    CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG
    GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT
    TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC
    TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT
    TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG
    AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA
    GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT
    ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC
    TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT
    CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC
    AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC
    CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA
    TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT
    TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG
    TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC
    CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG
    AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG
    CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG
    ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC
    CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA
    GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC
    CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC
    CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT
    CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT
    ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG
    CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG
    GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG
    ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC
    GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC
    ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA
    TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC
    ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA
    TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC
    TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT
    ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG
    ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA
    TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC
    ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC
    GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA
    CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG
    AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC
    CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC
    AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT
    TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT
    TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG
    GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC
    CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC
    AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT
    ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT
    CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC
    AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT
    CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA
    AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC
    ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC
    ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG
    TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT
    CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA
    TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT
    TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT
    AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA
    GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA
    AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT
    GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC
    TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG
    AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA
    GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC
    CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG
    CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA
    CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG
    AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA
    ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC
    GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC
    TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT
    CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC
    CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC
    TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG
    TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG
    GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA
    GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA
    GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA
    CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT
    TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG
    CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22981
    Clone ID Related Accession (Same CDS sequence) NM_001177310.1
    Accession Version NM_001177310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY333283.1, AF350881.2 and AL354795.17. Transcript Variant: This variant (b) contains an alternate 5' terminal exon compared to variant a. This results in a shorter isoform (b) with a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY333283.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATTATCC TATCTAAGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
    TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC
    TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT
    TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC
    ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT
    TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC
    ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA
    GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG
    CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG
    TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC
    TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC
    AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC
    GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG
    ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC
    CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG
    GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT
    TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC
    TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT
    TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG
    AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA
    GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT
    ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC
    TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT
    CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC
    AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC
    CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA
    TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT
    TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG
    TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC
    CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG
    AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG
    CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG
    ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC
    CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA
    GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC
    CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC
    CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT
    CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT
    ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG
    CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG
    GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG
    ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC
    GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC
    ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA
    TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC
    ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA
    TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC
    TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT
    ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG
    ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA
    TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC
    ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC
    GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA
    CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG
    AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC
    CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC
    AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT
    TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT
    TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG
    GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC
    CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC
    AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT
    ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT
    CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC
    AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT
    CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA
    AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC
    ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC
    ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG
    TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT
    CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA
    TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT
    TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT
    AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA
    GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA
    AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT
    GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC
    TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG
    AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA
    GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC
    CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG
    CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA
    CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG
    AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA
    ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC
    GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC
    TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT
    CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC
    CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC
    TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG
    TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG
    GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA
    GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA
    GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA
    CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT
    TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG
    CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001170781.1
    CDS73..6126
    Translation

    Target ORF information:

    RefSeq Version NM_001177310.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATTATCC TATCTAAGTC CCAGAAATCC TGGATTAAAG GAGTATTTGA CAAGAGAGAA 
    TGTAGCACAA TCATACCCAG CTCAAAAAAT CCTCACAGAT GTACTCCAGT ATGCCAAGTC
    TGCCAGAATT TAATCAGGTG TTACTGTGGC CGACTGATTG GAGACCATGC TGGGATAGAT
    TATTCCTGGA CCATCTCAGC TGCCAAGGGT AAAGAAAGTG AACAATGGTC TGTTGAAAAG
    CACACAACGA AAAGCCCAAC AGATACTTTT GGCACGATTA ATTTCCAAGA TGGAGAGCAC
    ACCCATCATG CCAAGTATAT TAGAACTTCT TATGATACAA AACTGGATCA TCTGTTACAT
    TTAATGTTGA AAGAGTGGAA AATGGAACTG CCCAAGCTTG TGATCTCAGT CCATGGGGGC
    ATCCAGAACT TTACTATGCC CTCTAAATTT AAAGAGATTT TCAGCCAAGG TTTGGTTAAA
    GCTGCAGAGA CAACAGGAGC GTGGATAATA ACTGAAGGCA TCAATACAGG AGTGTCCAAG
    CATGTTGGGG ATGCCTTGAA ATCCCATTCC TCTCATTCCT TGAGAAAAAT CTGGACAGTT
    GGAATCCCTC CTTGGGGTGT CATTGAGAAC CAGAGAGACC TTATTGGAAA AGATGTGGTG
    TGCCTGTACC AGACTCTGGA TAACCCCCTC AGCAAGCTCA CAACACTCAA CAGCATGCAC
    TCGCACTTCA TCCTGTCTGA TGATGGGACC GTGGGCAAGT ATGGAAATGA AATGAAGCTC
    AGAAGGAACC TGGAGAAGTA CCTCTCTCTG CAGAAAATAC ACTGCCGCTC AAGACAAGGC
    GTGCCGGTCG TGGGGCTGGT GGTGGAAGGC GGTCCCAACG TCATCCTGTC AGTGTGGGAG
    ACTGTCAAGG ACAAGGACCC AGTGGTGGTG TGTGAGGGCA CAGGTAGGGC GGCTGACCTC
    CTGGCCTTCA CACACAAACA CCTGGCAGAT GAAGGGATGC TGCGACCTCA GGTGAAAGAG
    GAGATCATCT GCATGATTCA GAACACTTTC AACTTTAGTC TTAAACAGTC CAAGCACCTT
    TTCCAAATTC TAATGGAGTG TATGGTTCAC AGGGATTGTA TTACCATATT TGATGCTGAC
    TCTGAAGAGC AGCAAGACCT GGACTTAGCA ATCCTAACAG CTTTGCTGAA GGGCACAAAT
    TTATCAGCGT CAGAGCAATT AAATCTGGCA ATGGCTTGGG ACAGGGTGGA CATTGCCAAG
    AAACATATCC TAATTTATGA ACAACACTGG AAGCCTGATG CCCTGGAACA AGCAATGTCA
    GATGCTTTAG TGATGGATCG GGTGGATTTT GTGAAGCTCT TAATAGAATA TGGAGTGAAC
    CTCCATCGCT TTCTTACCAT CCCTCGACTG GAAGAGCTCT ACAATACAAA ACAAGGACCT
    ACTAATACAC TCTTGCATCA TCTCGTCCAA GATGTGAAAC AGCATACCCT TCTTTCAGGC
    TACCGAATAA CCTTGATTGA CATTGGATTA GTAGTAGAAT ACCTCATTGG TAGAGCATAT
    CGCAGCAACT ACACTAGAAA ACATTTCAGA GCCCTCTACA ACAACCTCTA CAGAAAATAC
    AAGCACCAGA GACACTCCTC AGGAAATAGA AATGAGTCTG CAGAAAGTAC GCTGCACTCC
    CAGTTCATTA GAACTGCACA GCCATACAAA TTCAAGGAAA AGTCTATAGT CCTTCATAAA
    TCAAGGAAGA AGTCAAAAGA ACAAAATGTA TCAGATGACC CTGAGTCTAC TGGCTTTCTT
    TACCCTTACA ATGACCTGCT GGTTTGGGCT GTGCTGATGA AAAGGCAGAA GATGGCTATG
    TTCTTCTGGC AGCATGGAGA GGAGGCCACG GTTAAAGCCG TGATTGCGTG TATCCTCTAC
    CGGGCAATGG CCCATGAAGC TAAGGAGAGT CACATGGTGG ATGATGCCTC AGAAGAGTTG
    AAGAATTACT CAAAACAGTT TGGCCAGCTG GCTCTGGACT TGTTGGAGAA GGCATTCAAG
    CAGAATGAGC GCATGGCCAT GACGCTGTTG ACGTATGAAC TCAGGAACTG GAGCAATTCG
    ACCTGCCTTA AACTGGCCGT GTCGGGAGGA TTACGACCCT TTGTTTCACA TACTTGTACC
    CAGATGCTAC TGACAGACAT GTGGATGGGG AGGCTGAAAA TGAGGAAAAA CTCTTGGTTA
    AAGATTATTA TAAGCATTAT TTTACCACCC ACCATTTTGA CACTGGAATT TAAAAGCAAA
    GCTGAGATGT CACATGTTCC CCAGTCCCAG GACTTCCAAT TTATGTGGTA TTACAGTGAC
    CAGAACGCCA GCAGTTCCAA AGAAAGTGCT TCTGTGAAAG AGTATGATTT GGAAAGGGGC
    CATGATGAGA AACTGGATGA AAATCAGCAT TTTGGTTTGG AAAGTGGGCA CCAACACCTT
    CCGTGGACCA GGAAAGTCTA TGAGTTCTAC AGTGCTCCAA TTGTCAAGTT TTGGTTTTAT
    ACGATGGCGT ATTTGGCATT CCTCATGCTG TTCACTTACA CCGTGTTGGT GGAGATGCAG
    CCCCAGCCCA GCGTGCAGGA GTGGCTTGTT AGCATTTACA TCTTCACCAA TGCTATTGAG
    GTGGTCAGGG AGATCTGTAT TTCAGAACCT GGGAAGTTTA CCCAAAAGGT GAAGGTATGG
    ATTAGTGAGT ACTGGAACTT AACAGAAACT GTGGCCATTG GCCTGTTTTC AGCTGGCTTC
    GTCCTTCGAT GGGGTGACCC TCCTTTTCAC ACAGCGGGAA GACTGATCTA CTGCATAGAC
    ATCATATTCT GGTTCTCACG GCTCCTGGAC TTCTTTGCTG TGAATCAACA TGCAGGTCCA
    TATGTGACCA TGATTGCAAA AATGACAGCA AACATGTTCT ATATTGTGAT CATCATGGCC
    ATAGTCCTGC TGAGCTTTGG AGTGGCACGC AAGGCCATCC TTTCGCCAAA AGAGCCACCA
    TCTTGGAGTC TAGCTCGAGA TATTGTATTT GAGCCATACT GGATGATATA CGGAGAAGTC
    TATGCTGGAG AAATAGATGT TTGTTCAAGC CAGCCATCCT GCCCTCCTGG TTCTTTTCTT
    ACTCCATTCT TGCAAGCTGT CTACCTCTTC GTGCAATATA TCATCATGGT GAACCTGTTG
    ATTGCTTTCT TCAACAACGT TTACTTAGAT ATGGAATCCA TTTCAAATAA CCTGTGGAAA
    TACAACCGCT ATCGCTACAT CATGACCTAC CACGAGAAGC CCTGGCTGCC CCCACCTCTC
    ATCCTGCTGA GCCACGTGGG CCTTCTCCTC CGCCGCCTGT GCTGTCATCG AGCTCCTCAC
    GACCAAGAAG AGGGTGACGT TGGATTAAAA CTCTACCTCA GTAAGGAGGA TCTGAAAAAA
    CTTCATGATT TTGAGGAGCA GTGCGTGGAA AAATACTTCC ATGAGAAGAT GGAAGATGTG
    AATTGTAGTT GTGAGGAACG AATCCGAGTG ACATCAGAAA GGGTTACAGA GATGTACTTC
    CAGCTGAAAG AAATGAATGA AAAGGTGTCT TTTATAAAGG ACTCCTTACT GTCTTTGGAC
    AGCCAGGTGG GACACCTGCA GGATCTCTCT GCCCTGACTG TGGATACCCT GAAAGTCCTT
    TCTGCTGTTG ACACTTTGCA AGAGGATGAG GCTCTCCTGG CCAAGAGAAA GCATTCTACT
    TGCAAAAAAC TTCCCCACAG CTGGAGCAAT GTCATCTGTG CAGAGGTTCT AGGCAGCATG
    GAGATCGCTG GAGAGAAGAA ATACCAGTAT TATAGCATGC CCTCTTCTTT GCTGAGGAGC
    CTGGCTGGAG GCCGGCATCC CCCAAGAGTG CAGAGGGGGG CACTTCTTGA GATTACAAAC
    AGTAAAAGAG AGGCTACAAA TGTAAGAAAT GACCAGGAAA GGCAAGAAAC ACAAAGTAGT
    ATAGTGGTTT CTGGGGTGTC TCCTAACAGG CAAGCACACT CAAAGTATGG CCAGTTTCTT
    CTGGTCCCCT CTAATCTAAA GCGAGTTCCT TTTTCAGCAG AAACTGTCTT GCCTCTGTCC
    AGACCCTCTG TGCCAGATGT GCTGGCAACT GAACAGGACA TCCAGACTGA GGTTCTTGTT
    CATCTGACTG GGCAGACCCC AGTTGTCTCT GACTGGGCAT CAGTGGATGA ACCCAAGGAA
    AAGCACGAGC CTATTGCTCA CTTACTGGAT GGACAAGACA AGGCAGAGCA AGTGCTACCC
    ACTTTGAGTT GCACACCTGA ACCCATGACA ATGAGCTCCC CTCTTTCCCA AGCCAAGATC
    ATGCAAACTG GAGGTGGATA TGTAAACTGG GCATTTTCAG AAGGTGATGA AACTGGTGTG
    TTTAGCATCA AGAAAAAGTG GCAAACCTGC TTGCCCTCCA CTTGTGACAG TGATTCCTCT
    CGGAGTGAAC AGCACCAGAA GCAGGCCCAG GACAGCTCCC TATCTGATAA CTCAACAAGA
    TCGGCCCAGA GTAGTGAATG CTCAGAGGTG GGACCATGGC TTCAGCCAAA CACATCCTTT
    TGGATCAATC CTCTCCGCAG ATACAGGCCC TTCGCTAGGA GTCATAGTTT TAGATTCCAT
    AAGGAGGAGA AATTGATGAA GATCTGTAAG ATTAAAAATC TTTCAGGCTC TTCAGAAATA
    GGGCAGGGAG CATGGGTCAA AGCGAAAATG CTAACCAAAG ACAGGAGACT GTCAAAGAAA
    AAGAAGAATA CTCAAGGACT CCAGGTGCCA ATCATAACAG TCAATGCCTG CTCTCAGAGT
    GACCAGTTGA ATCCAGAGCC AGGAGAAAAC AGCATCTCTG AAGAGGAGTA CAGCAAGAAC
    TGGTTCACAG TGTCCAAATT TAGTCACACA GGTGTAGAAC CTTACATACA TCAGAAAATG
    AAAACTAAAG AAATTGGACA ATGTGCTATA CAAATCAGTG ATTACCTAAA GCAGTCTCAA
    GAGGATCTCA GCAAAAACTC TTTGTGGAAT TCCAGGAGCA CCAACCTCAA TAGGAACTCC
    CTGCTGAAAA GTTCAATTGG AGTTGACAAG ATCTCAGCCT CCTTAAAAAG CCCTCAAGAG
    CCTCACCATC ATTATTCAGC CATTGAAAGG AATAATTTAA TGAGGCTTTC TCAGACCATA
    CCATTTACAC CAGTCCAACT GTTTGCAGGA GAAGAAATAA CTGTCTACAG GTTGGAGGAG
    AGTTCCCCTT TAAACCTTGA TAAAAGCATG TCCTCTTGGT CTCAGCGTGG GAGAGCGGCA
    ATGATCCAGG TATTGTCCCG AGAGGAGATG GATGGGGGCC TCCGTAAAGC TATGAGAGTC
    GTCAGCACTT GGTCTGAGGA TGACATTCTC AAGCCGGGAC AAGTTTTCAT TGTCAAGTCC
    TTTCTTCCTG AGGTTGTGCG GACATGGCAT AAAATCTTCC AGGAGAGCAC TGTGCTTCAT
    CTTTGCCTCA GGGAAATTCA ACAACAAAGA GCTGCTCAAA AATTGATCTA TACCTTCAAC
    CAAGTGAAAC CACAAACCAT ACCCTACACA CCAAGGTTCC TGGAAGTTTT CTTAATCTAC
    TGCCATTCAG CCAACCAGTG GTTGACCATT GAGAAGTATA TGACAGGGGA GTTCCGGAAG
    TATAACAACA ACAATGGTGA TGAAATCACC CCCACCAACA CCCTGGAGGA GCTGATGTTG
    GCTTTCTCTC ACTGGACCTA TGAGTACACT CGGGGAGAGC TGCTGGTTTT AGATTTGCAA
    GGTGTTGGAG AAAATTTGAC AGATCCATCT GTTATAAAAC CTGAAGTCAA ACAATCAAGA
    GGAATGGTGT TTGGACCGGC CAATTTGGGG GAAGATGCAA TTAGAAACTT CATTGCAAAA
    CATCATTGTA ACTCCTGCTG CCGGAAGCTC AAACTCCCGG ATTTAAAAAG AAATGACTAT
    TCCCCTGAAA GGATAAATTC CACCTTTGGA CTTGAGATAA AAATAGAATC AGCTGAGGAG
    CCTCCAGCAA GGGAGACGGG TAGAAATTCC CCAGAAGATG ATATGCAACT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu75939
    Clone ID Related Accession (Same CDS sequence) XM_011518244.1
    Accession Version XM_011518244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG GAGAAGAAAT AACTGTCTAC
    AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT
    GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA
    GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC
    ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC
    ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC
    TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT
    TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG
    GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG
    GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT
    TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC
    AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC
    TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA
    AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA
    TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA
    CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011516546.1
    CDS239..6304
    Translation

    Target ORF information:

    RefSeq Version XM_011518244.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAAAGAAC AACCTGTCTT GGAGCGCTTG CAGTCCCAGA AATCCTGGAT TAAAGGAGTA 
    TTTGACAAGA GAGAATGTAG CACAATCATA CCCAGCTCAA AAAATCCTCA CAGATGTACT
    CCAGTATGCC AAGTCTGCCA GAATTTAATC AGGTGTTACT GTGGCCGACT GATTGGAGAC
    CATGCTGGGA TAGATTATTC CTGGACCATC TCAGCTGCCA AGGGTAAAGA AAGTGAACAA
    TGGTCTGTTG AAAAGCACAC AACGAAAAGC CCAACAGATA CTTTTGGCAC GATTAATTTC
    CAAGATGGAG AGCACACCCA TCATGCCAAG TATATTAGAA CTTCTTATGA TACAAAACTG
    GATCATCTGT TACATTTAAT GTTGAAAGAG TGGAAAATGG AACTGCCCAA GCTTGTGATC
    TCAGTCCATG GGGGCATCCA GAACTTTACT ATGCCCTCTA AATTTAAAGA GATTTTCAGC
    CAAGGTTTGG TTAAAGCTGC AGAGACAACA GGAGCGTGGA TAATAACTGA AGGCATCAAT
    ACAGGAGTGT CCAAGCATGT TGGGGATGCC TTGAAATCCC ATTCCTCTCA TTCCTTGAGA
    AAAATCTGGA CAGTTGGAAT CCCTCCTTGG GGTGTCATTG AGAACCAGAG AGACCTTATT
    GGAAAAGATG TGGTGTGCCT GTACCAGACT CTGGATAACC CCCTCAGCAA GCTCACAACA
    CTCAACAGCA TGCACTCGCA CTTCATCCTG TCTGATGATG GGACCGTGGG CAAGTATGGA
    AATGAAATGA AGCTCAGAAG GAACCTGGAG AAGTACCTCT CTCTGCAGAA AATACACTGC
    CGCTCAAGAC AAGGCGTGCC GGTCGTGGGG CTGGTGGTGG AAGGCGGTCC CAACGTCATC
    CTGTCAGTGT GGGAGACTGT CAAGGACAAG GACCCAGTGG TGGTGTGTGA GGGCACAGGT
    AGGGCGGCTG ACCTCCTGGC CTTCACACAC AAACACCTGG CAGATGAAGG GATGCTGCGA
    CCTCAGGTGA AAGAGGAGAT CATCTGCATG ATTCAGAACA CTTTCAACTT TAGTCTTAAA
    CAGTCCAAGC ACCTTTTCCA AATTCTAATG GAGTGTATGG TTCACAGGGA TTGTATTACC
    ATATTTGATG CTGACTCTGA AGAGCAGCAA GACCTGGACT TAGCAATCCT AACAGCTTTG
    CTGAAGGGCA CAAATTTATC AGCGTCAGAG CAATTAAATC TGGCAATGGC TTGGGACAGG
    GTGGACATTG CCAAGAAACA TATCCTAATT TATGAACAAC ACTGGAAGCC TGATGCCCTG
    GAACAAGCAA TGTCAGATGC TTTAGTGATG GATCGGGTGG ATTTTGTGAA GCTCTTAATA
    GAATATGGAG TGAACCTCCA TCGCTTTCTT ACCATCCCTC GACTGGAAGA GCTCTACAAT
    ACAAAACAAG GACCTACTAA TACACTCTTG CATCATCTCG TCCAAGATGT GAAACAGCAT
    ACCCTTCTTT CAGGCTACCG AATAACCTTG ATTGACATTG GATTAGTAGT AGAATACCTC
    ATTGGTAGAG CATATCGCAG CAACTACACT AGAAAACATT TCAGAGCCCT CTACAACAAC
    CTCTACAGAA AATACAAGCA CCAGAGACAC TCCTCAGGAA ATAGAAATGA GTCTGCAGAA
    AGTACGCTGC ACTCCCAGTT CATTAGAACT GCACAGCCAT ACAAATTCAA GGAAAAGTCT
    ATAGTCCTTC ATAAATCAAG GAAGAAGTCA AAAGAACAAA ATGTATCAGA TGACCCTGAG
    TCTACTGGCT TTCTTTACCC TTACAATGAC CTGCTGGTTT GGGCTGTGCT GATGAAAAGG
    CAGAAGATGG CTATGTTCTT CTGGCAGCAT GGAGAGGAGG CCACGGTTAA AGCCGTGATT
    GCGTGTATCC TCTACCGGGC AATGGCCCAT GAAGCTAAGG AGAGTCACAT GGTGGATGAT
    GCCTCAGAAG AGTTGAAGAA TTACTCAAAA CAGTTTGGCC AGCTGGCTCT GGACTTGTTG
    GAGAAGGCAT TCAAGCAGAA TGAGCGCATG GCCATGACGC TGTTGACGTA TGAACTCAGG
    AACTGGAGCA ATTCGACCTG CCTTAAACTG GCCGTGTCGG GAGGATTACG ACCCTTTGTT
    TCACATACTT GTACCCAGAT GCTACTGACA GACATGTGGA TGGGGAGGCT GAAAATGAGG
    AAAAACTCTT GGTTAAAGAT TATTATAAGC ATTATTTTAC CACCCACCAT TTTGACACTG
    GAATTTAAAA GCAAAGCTGA GATGTCACAT GTTCCCCAGT CCCAGGACTT CCAATTTATG
    TGGTATTACA GTGACCAGAA CGCCAGCAGT TCCAAAGAAA GTGCTTCTGT GAAAGAGTAT
    GATTTGGAAA GGGGCCATGA TGAGAAACTG GATGAAAATC AGCATTTTGG TTTGGAAAGT
    GGGCACCAAC ACCTTCCGTG GACCAGGAAA GTCTATGAGT TCTACAGTGC TCCAATTGTC
    AAGTTTTGGT TTTATACGAT GGCGTATTTG GCATTCCTCA TGCTGTTCAC TTACACCGTG
    TTGGTGGAGA TGCAGCCCCA GCCCAGCGTG CAGGAGTGGC TTGTTAGCAT TTACATCTTC
    ACCAATGCTA TTGAGGTGGT CAGGGAGATC TGTATTTCAG AACCTGGGAA GTTTACCCAA
    AAGGTGAAGG TATGGATTAG TGAGTACTGG AACTTAACAG AAACTGTGGC CATTGGCCTG
    TTTTCAGCTG GCTTCGTCCT TCGATGGGGT GACCCTCCTT TTCACACAGC GGGAAGACTG
    ATCTACTGCA TAGACATCAT ATTCTGGTTC TCACGGCTCC TGGACTTCTT TGCTGTGAAT
    CAACATGCAG GTCCATATGT GACCATGATT GCAAAAATGA CAGCAAACAT GTTCTATATT
    GTGATCATCA TGGCCATAGT CCTGCTGAGC TTTGGAGTGG CACGCAAGGC CATCCTTTCG
    CCAAAAGAGC CACCATCTTG GAGTCTAGCT CGAGATATTG TATTTGAGCC ATACTGGATG
    ATATACGGAG AAGTCTATGC TGGAGAAATA GATGTTTGTT CAAGCCAGCC ATCCTGCCCT
    CCTGGTTCTT TTCTTACTCC ATTCTTGCAA GCTGTCTACC TCTTCGTGCA ATATATCATC
    ATGGTGAACC TGTTGATTGC TTTCTTCAAC AACGTTTACT TAGATATGGA ATCCATTTCA
    AATAACCTGT GGAAATACAA CCGCTATCGC TACATCATGA CCTACCACGA GAAGCCCTGG
    CTGCCCCCAC CTCTCATCCT GCTGAGCCAC GTGGGCCTTC TCCTCCGCCG CCTGTGCTGT
    CATCGAGCTC CTCACGACCA AGAAGAGGGT GACGTTGGAT TAAAACTCTA CCTCAGTAAG
    GAGGATCTGA AAAAACTTCA TGATTTTGAG GAGCAGTGCG TGGAAAAATA CTTCCATGAG
    AAGATGGAAG ATGTGAATTG TAGTTGTGAG GAACGAATCC GAGTGACATC AGAAAGGGTT
    ACAGAGATGT ACTTCCAGCT GAAAGAAATG AATGAAAAGG TGTCTTTTAT AAAGGACTCC
    TTACTGTCTT TGGACAGCCA GGTGGGACAC CTGCAGGATC TCTCTGCCCT GACTGTGGAT
    ACCCTGAAAG TCCTTTCTGC TGTTGACACT TTGCAAGAGG ATGAGGCTCT CCTGGCCAAG
    AGAAAGCATT CTACTTGCAA AAAACTTCCC CACAGCTGGA GCAATGTCAT CTGTGCAGAG
    GTTCTAGGCA GCATGGAGAT CGCTGGAGAG AAGAAATACC AGTATTATAG CATGCCCTCT
    TCTTTGCTGA GGAGCCTGGC TGGAGGCCGG CATCCCCCAA GAGTGCAGAG GGGGGCACTT
    CTTGAGATTA CAAACAGTAA AAGAGAGGCT ACAAATGTAA GAAATGACCA GGAAAGGCAA
    GAAACACAAA GTAGTATAGT GGTTTCTGGG GTGTCTCCTA ACAGGCAAGC ACACTCAAAG
    TATGGCCAGT TTCTTCTGGT CCCCTCTAAT CTAAAGCGAG TTCCTTTTTC AGCAGAAACT
    GTCTTGCCTC TGTCCAGACC CTCTGTGCCA GATGTGCTGG CAACTGAACA GGACATCCAG
    ACTGAGGTTC TTGTTCATCT GACTGGGCAG ACCCCAGTTG TCTCTGACTG GGCATCAGTG
    GATGAACCCA AGGAAAAGCA CGAGCCTATT GCTCACTTAC TGGATGGACA AGACAAGGCA
    GAGCAAGTGC TACCCACTTT GAGTTGCACA CCTGAACCCA TGACAATGAG CTCCCCTCTT
    TCCCAAGCCA AGATCATGCA AACTGGAGGT GGATATGTAA ACTGGGCATT TTCAGAAGGT
    GATGAAACTG GTGTGTTTAG CATCAAGAAA AAGTGGCAAA CCTGCTTGCC CTCCACTTGT
    GACAGTGATT CCTCTCGGAG TGAACAGCAC CAGAAGCAGG CCCAGGACAG CTCCCTATCT
    GATAACTCAA CAAGATCGGC CCAGAGTAGT GAATGCTCAG AGGTGGGACC ATGGCTTCAG
    CCAAACACAT CCTTTTGGAT CAATCCTCTC CGCAGATACA GGCCCTTCGC TAGGAGTCAT
    AGTTTTAGAT TCCATAAGGA GGAGAAATTG ATGAAGATCT GTAAGATTAA AAATCTTTCA
    GGCTCTTCAG AAATAGGGCA GGGAGCATGG GTCAAAGCGA AAATGCTAAC CAAAGACAGG
    AGACTGTCAA AGAAAAAGAA GAATACTCAA GGACTCCAGG TGCCAATCAT AACAGTCAAT
    GCCTGCTCTC AGAGTGACCA GTTGAATCCA GAGCCAGGAG AAAACAGCAT CTCTGAAGAG
    GAGTACAGCA AGAACTGGTT CACAGTGTCC AAATTTAGTC ACACAGGTGT AGAACCTTAC
    ATACATCAGA AAATGAAAAC TAAAGAAATT GGACAATGTG CTATACAAAT CAGTGATTAC
    CTAAAGCAGT CTCAAGAGGA TCTCAGCAAA AACTCTTTGT GGAATTCCAG GAGCACCAAC
    CTCAATAGGA ACTCCCTGCT GAAAAGTTCA ATTGGAGTTG ACAAGATCTC AGCCTCCTTA
    AAAAGCCCTC AAGAGCCTCA CCATCATTAT TCAGCCATTG AAAGGAATAA TTTAATGAGG
    CTTTCTCAGA CCATACCATT TACACCAGTC CAACTGTTTG GAGAAGAAAT AACTGTCTAC
    AGGTTGGAGG AGAGTTCCCC TTTAAACCTT GATAAAAGCA TGTCCTCTTG GTCTCAGCGT
    GGGAGAGCGG CAATGATCCA GGTATTGTCC CGAGAGGAGA TGGATGGGGG CCTCCGTAAA
    GCTATGAGAG TCGTCAGCAC TTGGTCTGAG GATGACATTC TCAAGCCGGG ACAAGTTTTC
    ATTGTCAAGT CCTTTCTTCC TGAGGTTGTG CGGACATGGC ATAAAATCTT CCAGGAGAGC
    ACTGTGCTTC ATCTTTGCCT CAGGGAAATT CAACAACAAA GAGCTGCTCA AAAATTGATC
    TATACCTTCA ACCAAGTGAA ACCACAAACC ATACCCTACA CACCAAGGTT CCTGGAAGTT
    TTCTTAATCT ACTGCCATTC AGCCAACCAG TGGTTGACCA TTGAGAAGTA TATGACAGGG
    GAGTTCCGGA AGTATAACAA CAACAATGGT GATGAAATCA CCCCCACCAA CACCCTGGAG
    GAGCTGATGT TGGCTTTCTC TCACTGGACC TATGAGTACA CTCGGGGAGA GCTGCTGGTT
    TTAGATTTGC AAGGTGTTGG AGAAAATTTG ACAGATCCAT CTGTTATAAA ACCTGAAGTC
    AAACAATCAA GAGGAATGGT GTTTGGACCG GCCAATTTGG GGGAAGATGC AATTAGAAAC
    TTCATTGCAA AACATCATTG TAACTCCTGC TGCCGGAAGC TCAAACTCCC GGATTTAAAA
    AGAAATGACT ATTCCCCTGA AAGGATAAAT TCCACCTTTG GACTTGAGAT AAAAATAGAA
    TCAGCTGAGG AGCCTCCAGC AAGGGAGACG GGTAGAAATT CCCCAGAAGA TGATATGCAA
    CTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.