COL6A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A1
Entrez Gene ID	1291
Full Name	collagen type VI alpha 1 chain
Synonyms	BTHLM1,OPLL,UCHMD1
General protein information	Preferred Names collagen type VI alpha 1 chain Names collagen alpha-1(VI) chain alpha 1 (VI) chain (61 AA) collagen VI, alpha-1 polypeptide collagen, type VI, alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.3
Summary	The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008].
Disorder MIM:	120220
Disorder Html:	Bethlem myopathy 1, 158810 (3); Ullrich congenital muscular dystrophy 1, 254090 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001848.3	NP_001839.2	collagen alpha-1(VI) chain precursor
NM_001848.2	NP_001839.2	collagen alpha-1(VI) chain precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col6a1	XP_215375.5
Xenopus tropicalis (tropical clawed frog)	col6a1	XP_002932077.2
Canis lupus familiaris (dog)	COL6A1	XP_003434049.2
Mus musculus (house mouse)	Col6a1	NP_034063.1
Gallus gallus (chicken)	COL6A1	NP_990438.1
Danio rerio (zebrafish)	col6a1	XP_698253.3
Homo sapiens (human)	COL6A1	NP_001839.2
Pan troglodytes (chimpanzee)	COL6A1	XP_001158445.1
	LOC721855	XP_001118050.2
Bos taurus (cattle)	COL6A1	NP_001137337.1

Gene Ontology

The following COL6A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17965	NM_001848.3 Latest version!	Homo sapiens collagen type VI alpha 1 chain (COL6A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu17965	NM_001848.2	Homo sapiens collagen type VI alpha 1 chain (COL6A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A1 ( NM_001848.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001839.2 Homo sapiens collagen type VI alpha 1 chain (COL6A1), mRNA.

Price & Availability↑

CloneID	OHu17965
Clone ID Related Accession (Same CDS sequence)	NM_001848.3 , NM_001848.2
Accession Version	NM_001848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	collagen alpha-1(VI) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU100220.1, CN369026.1, BC052575.1, BI769554.1, CN369002.1, CN369041.1, BM686857.1 and BM707144.1. On Aug 31, 2019 this sequence version replaced NM_001848.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052575.1, GQ891373.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCGG CCCGTGCTCT GCTGCCCCTG CTGCTGCAGG CCTGCTGGAC AGCCGCGCAG 
GATGAGCCGG AGACCCCGAG GGCCGTGGCC TTCCAGGACT GCCCCGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCTGAGAG CGTGGCCCTG AGGCTGAAGC CCTACGGGGC CCTCGTGGAC 
AAAGTCAAGT CCTTCACCAA GCGCTTCATC GACAACCTGA GGGACAGGTA CTACCGCTGT 
GACCGAAACC TGGTGTGGAA CGCAGGCGCG CTGCACTACA GTGACGAGGT GGAGATCATC 
CAAGGCCTCA CGCGCATGCC TGGCGGCCGC GACGCACTCA AAAGCAGCGT GGACGCGGTC 
AAGTACTTTG GGAAGGGCAC CTACACCGAC TGCGCTATCA AGAAGGGGCT GGAGCAGCTC 
CTCGTGGGGG GCTCCCACCT GAAGGAGAAT AAGTACCTGA TTGTGGTGAC CGACGGGCAC 
CCCCTGGAGG GCTACAAGGA ACCCTGTGGG GGGCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CACCTGGGCG TCAAAGTCTT CTCGGTGGCC ATCACACCCG ACCACCTGGA GCCGCGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGCCAG 
AGCCGCGACG CAGAGGAGGC CATCAGCCAG ACCATCGACA CCATCGTGGA CATGATCAAA 
AATAACGTGG AGCAAGTGTG CTGCTCCTTC GAATGCCAGC CTGCAAGAGG ACCTCCGGGG 
CTCCGGGGCG ACCCCGGCTT TGAGGGAGAA CGAGGCAAGC CGGGGCTCCC AGGAGAGAAG 
GGAGAAGCCG GAGATCCTGG AAGACCCGGG GACCTCGGAC CTGTTGGGTA CCAGGGAATG 
AAGGGAGAAA AAGGGAGCCG TGGGGAGAAG GGCTCCAGGG GACCCAAGGG CTACAAGGGA 
GAGAAGGGCA AGCGTGGCAT CGACGGGGTG GACGGCGTGA AGGGGGAGAT GGGGTACCCA 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGG TTTGACGGCA TTCAAGGACC CCCTGGCCCC 
AAGGGAGACC CCGGTGCCTT TGGACTGAAA GGAGAAAAGG GCGAGCCTGG AGCTGACGGG 
GAGGCGGGGA GACCAGGGAG CTCGGGACCA TCTGGAGACG AGGGCCAGCC GGGAGAGCCT 
GGGCCCCCCG GAGAGAAAGG AGAGGCGGGC GACGAGGGGA ACCCAGGACC TGACGGTGCC 
CCCGGGGAGC GGGGTGGCCC TGGAGAGAGA GGACCACGGG GGACCCCAGG CACGCGGGGA 
CCAAGAGGAG ACCCTGGTGA AGCTGGCCCG CAGGGTGATC AGGGAAGAGA AGGCCCCGTT 
GGTGTCCCTG GAGACCCGGG CGAGGCTGGC CCTATCGGAC CTAAAGGCTA CCGAGGCGAT 
GAGGGTCCCC CAGGGTCCGA GGGTGCCAGA GGAGCCCCAG GACCTGCCGG ACCCCCTGGA 
GACCCGGGGC TGATGGGTGA AAGGGGAGAA GACGGCCCCG CTGGAAATGG CACCGAGGGC 
TTCCCCGGCT TCCCCGGGTA TCCGGGCAAC AGGGGCGCTC CCGGGATAAA CGGCACGAAG 
GGCTACCCCG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA CGATAACAAC 
GACATTGCAC CCCGAGGAGT CAAAGGAGCA AAGGGGTACC GGGGTCCCGA GGGCCCCCAG 
GGACCCCCAG GACACCAAGG ACCGCCTGGG CCGGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAATGCAAG TGCGGCCCCA TCGACCTCCT GTTCGTGCTG 
GACAGCTCAG AGAGCATTGG CCTGCAGAAC TTCGAGATTG CCAAGGACTT CGTCGTCAAG 
GTCATCGACC GGCTGAGCCG GGACGAGCTG GTCAAGTTCG AGCCAGGGCA GTCGTACGCG 
GGTGTGGTGC AGTACAGCCA CAGCCAGATG CAGGAGCACG TGAGCCTGCG CAGCCCCAGC 
ATCCGGAACG TGCAGGAGCT CAAGGAAGCC ATCAAGAGCC TGCAGTGGAT GGCGGGCGGC 
ACCTTCACGG GGGAGGCCCT GCAGTACACG CGGGACCAGC TGCTGCCGCC CAGCCCGAAC 
AACCGCATCG CCCTGGTCAT CACTGACGGG CGCTCAGACA CTCAGAGGGA CACCACACCG 
CTCAACGTGC TCTGCAGCCC CGGCATCCAG GTGGTCTCCG TGGGCATCAA AGACGTGTTT 
GACTTCATCC CAGGCTCAGA CCAGCTCAAT GTCATTTCTT GCCAAGGCCT GGCACCATCC 
CAGGGCCGGC CCGGCCTCTC GCTGGTCAAG GAGAACTATG CAGAGCTGCT GGAGGATGCC 
TTCCTGAAGA ATGTCACCGC CCAGATCTGC ATAGACAAGA AGTGTCCAGA TTACACCTGC 
CCCATCACGT TCTCCTCCCC GGCTGACATC ACCATCCTGC TGGACGGCTC CGCCAGCGTG 
GGCAGCCACA ACTTTGACAC CACCAAGCGC TTCGCCAAGC GCCTGGCCGA GCGCTTCCTC 
ACAGCGGGCA GGACGGACCC CGCCCACGAC GTGCGGGTGG CGGTGGTGCA GTACAGCGGC 
ACGGGCCAGC AGCGCCCAGA GCGGGCGTCG CTGCAGTTCC TGCAGAACTA CACGGCCCTG 
GCCAGTGCCG TCGATGCCAT GGACTTTATC AACGACGCCA CCGACGTCAA CGATGCCCTG 
GGCTATGTGA CCCGCTTCTA CCGCGAGGCC TCGTCCGGCG CTGCCAAGAA GAGGCTGCTG 
CTCTTCTCAG ATGGCAACTC GCAGGGCGCC ACGCCCGCTG CCATCGAGAA GGCCGTGCAG 
GAAGCCCAGC GGGCAGGCAT CGAGATCTTC GTGGTGGTCG TGGGCCGCCA GGTGAATGAG 
CCCCACATCC GCGTCCTGGT CACCGGCAAG ACGGCCGAGT ACGACGTGGC CTACGGCGAG 
AGCCACCTGT TCCGTGTCCC CAGCTACCAG GCCCTGCTCC GCGGTGTCTT CCACCAGACA 
GTCTCCAGGA AGGTGGCGCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001839.2
CDS	82..3168
Translation	MRAARALLPLLLQACWTAAQDEPETPRAVAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCDRNLVWNAGALHYSDEVEIIQGLTRMPGGRDALKSSVDAVKYFGKGTYTDCAIKKGLEQLLVGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGVKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTIVDMIKNNVEQVCCSFECQPARGPPGLRGDPGFEGERGKPGLPGEKGEAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGIDGVDGVKGEMGYPGLPGCKGSPGFDGIQGPPGPKGDPGAFGLKGEKGEPGADGEAGRPGSSGPSGDEGQPGEPGPPGEKGEAGDEGNPGPDGAPGERGGPGERGPRGTPGTRGPRGDPGEAGPQGDQGREGPVGVPGDPGEAGPIGPKGYRGDEGPPGSEGARGAPGPAGPPGDPGLMGERGEDGPAGNGTEGFPGFPGYPGNRGAPGINGTKGYPGLKGDEGEAGDPGDDNNDIAPRGVKGAKGYRGPEGPQGPPGHQGPPGPDECEILDIIMKMCSCCECKCGPIDLLFVLDSSESIGLQNFEIAKDFVVKVIDRLSRDELVKFEPGQSYAGVVQYSHSQMQEHVSLRSPSIRNVQELKEAIKSLQWMAGGTFTGEALQYTRDQLLPPSPNNRIALVITDGRSDTQRDTTPLNVLCSPGIQVVSVGIKDVFDFIPGSDQLNVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG

Target ORF information:

RefSeq Version	NM_001848.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 1 chain (COL6A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001848.3

ORF Insert Sequence:

ATGAGGGCGG CCCGTGCTCT GCTGCCCCTG CTGCTGCAGG CCTGCTGGAC AGCCGCGCAG 
GATGAGCCGG AGACCCCGAG GGCCGTGGCC TTCCAGGACT GCCCCGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCTGAGAG CGTGGCCCTG AGGCTGAAGC CCTACGGGGC CCTCGTGGAC 
AAAGTCAAGT CCTTCACCAA GCGCTTCATC GACAACCTGA GGGACAGGTA CTACCGCTGT 
GACCGAAACC TGGTGTGGAA CGCAGGCGCG CTGCACTACA GTGACGAGGT GGAGATCATC 
CAAGGCCTCA CGCGCATGCC TGGCGGCCGC GACGCACTCA AAAGCAGCGT GGACGCGGTC 
AAGTACTTTG GGAAGGGCAC CTACACCGAC TGCGCTATCA AGAAGGGGCT GGAGCAGCTC 
CTCGTGGGGG GCTCCCACCT GAAGGAGAAT AAGTACCTGA TTGTGGTGAC CGACGGGCAC 
CCCCTGGAGG GCTACAAGGA ACCCTGTGGG GGGCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CACCTGGGCG TCAAAGTCTT CTCGGTGGCC ATCACACCCG ACCACCTGGA GCCGCGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGCCAG 
AGCCGCGACG CAGAGGAGGC CATCAGCCAG ACCATCGACA CCATCGTGGA CATGATCAAA 
AATAACGTGG AGCAAGTGTG CTGCTCCTTC GAATGCCAGC CTGCAAGAGG ACCTCCGGGG 
CTCCGGGGCG ACCCCGGCTT TGAGGGAGAA CGAGGCAAGC CGGGGCTCCC AGGAGAGAAG 
GGAGAAGCCG GAGATCCTGG AAGACCCGGG GACCTCGGAC CTGTTGGGTA CCAGGGAATG 
AAGGGAGAAA AAGGGAGCCG TGGGGAGAAG GGCTCCAGGG GACCCAAGGG CTACAAGGGA 
GAGAAGGGCA AGCGTGGCAT CGACGGGGTG GACGGCGTGA AGGGGGAGAT GGGGTACCCA 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGG TTTGACGGCA TTCAAGGACC CCCTGGCCCC 
AAGGGAGACC CCGGTGCCTT TGGACTGAAA GGAGAAAAGG GCGAGCCTGG AGCTGACGGG 
GAGGCGGGGA GACCAGGGAG CTCGGGACCA TCTGGAGACG AGGGCCAGCC GGGAGAGCCT 
GGGCCCCCCG GAGAGAAAGG AGAGGCGGGC GACGAGGGGA ACCCAGGACC TGACGGTGCC 
CCCGGGGAGC GGGGTGGCCC TGGAGAGAGA GGACCACGGG GGACCCCAGG CACGCGGGGA 
CCAAGAGGAG ACCCTGGTGA AGCTGGCCCG CAGGGTGATC AGGGAAGAGA AGGCCCCGTT 
GGTGTCCCTG GAGACCCGGG CGAGGCTGGC CCTATCGGAC CTAAAGGCTA CCGAGGCGAT 
GAGGGTCCCC CAGGGTCCGA GGGTGCCAGA GGAGCCCCAG GACCTGCCGG ACCCCCTGGA 
GACCCGGGGC TGATGGGTGA AAGGGGAGAA GACGGCCCCG CTGGAAATGG CACCGAGGGC 
TTCCCCGGCT TCCCCGGGTA TCCGGGCAAC AGGGGCGCTC CCGGGATAAA CGGCACGAAG 
GGCTACCCCG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA CGATAACAAC 
GACATTGCAC CCCGAGGAGT CAAAGGAGCA AAGGGGTACC GGGGTCCCGA GGGCCCCCAG 
GGACCCCCAG GACACCAAGG ACCGCCTGGG CCGGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAATGCAAG TGCGGCCCCA TCGACCTCCT GTTCGTGCTG 
GACAGCTCAG AGAGCATTGG CCTGCAGAAC TTCGAGATTG CCAAGGACTT CGTCGTCAAG 
GTCATCGACC GGCTGAGCCG GGACGAGCTG GTCAAGTTCG AGCCAGGGCA GTCGTACGCG 
GGTGTGGTGC AGTACAGCCA CAGCCAGATG CAGGAGCACG TGAGCCTGCG CAGCCCCAGC 
ATCCGGAACG TGCAGGAGCT CAAGGAAGCC ATCAAGAGCC TGCAGTGGAT GGCGGGCGGC 
ACCTTCACGG GGGAGGCCCT GCAGTACACG CGGGACCAGC TGCTGCCGCC CAGCCCGAAC 
AACCGCATCG CCCTGGTCAT CACTGACGGG CGCTCAGACA CTCAGAGGGA CACCACACCG 
CTCAACGTGC TCTGCAGCCC CGGCATCCAG GTGGTCTCCG TGGGCATCAA AGACGTGTTT 
GACTTCATCC CAGGCTCAGA CCAGCTCAAT GTCATTTCTT GCCAAGGCCT GGCACCATCC 
CAGGGCCGGC CCGGCCTCTC GCTGGTCAAG GAGAACTATG CAGAGCTGCT GGAGGATGCC 
TTCCTGAAGA ATGTCACCGC CCAGATCTGC ATAGACAAGA AGTGTCCAGA TTACACCTGC 
CCCATCACGT TCTCCTCCCC GGCTGACATC ACCATCCTGC TGGACGGCTC CGCCAGCGTG 
GGCAGCCACA ACTTTGACAC CACCAAGCGC TTCGCCAAGC GCCTGGCCGA GCGCTTCCTC 
ACAGCGGGCA GGACGGACCC CGCCCACGAC GTGCGGGTGG CGGTGGTGCA GTACAGCGGC 
ACGGGCCAGC AGCGCCCAGA GCGGGCGTCG CTGCAGTTCC TGCAGAACTA CACGGCCCTG 
GCCAGTGCCG TCGATGCCAT GGACTTTATC AACGACGCCA CCGACGTCAA CGATGCCCTG 
GGCTATGTGA CCCGCTTCTA CCGCGAGGCC TCGTCCGGCG CTGCCAAGAA GAGGCTGCTG 
CTCTTCTCAG ATGGCAACTC GCAGGGCGCC ACGCCCGCTG CCATCGAGAA GGCCGTGCAG 
GAAGCCCAGC GGGCAGGCAT CGAGATCTTC GTGGTGGTCG TGGGCCGCCA GGTGAATGAG 
CCCCACATCC GCGTCCTGGT CACCGGCAAG ACGGCCGAGT ACGACGTGGC CTACGGCGAG 
AGCCACCTGT TCCGTGTCCC CAGCTACCAG GCCCTGCTCC GCGGTGTCTT CCACCAGACA 
GTCTCCAGGA AGGTGGCGCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A1 ( NM_001848.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001839.2 Homo sapiens collagen type VI alpha 1 chain (COL6A1), mRNA.

Price & Availability↑

CloneID	OHu17965
Clone ID Related Accession (Same CDS sequence)	NM_001848.3 , NM_001848.2
Accession Version	NM_001848.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	collagen alpha-1(VI) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU100220.1, CN369026.1, BC052575.1, BI769554.1, CN369002.1, CN369041.1, BM686857.1 and BM707144.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2006 this sequence version replaced NM_001848.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.261776.1, BC052575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCGG CCCGTGCTCT GCTGCCCCTG CTGCTGCAGG CCTGCTGGAC AGCCGCGCAG 
GATGAGCCGG AGACCCCGAG GGCCGTGGCC TTCCAGGACT GCCCCGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCTGAGAG CGTGGCCCTG AGGCTGAAGC CCTACGGGGC CCTCGTGGAC 
AAAGTCAAGT CCTTCACCAA GCGCTTCATC GACAACCTGA GGGACAGGTA CTACCGCTGT 
GACCGAAACC TGGTGTGGAA CGCAGGCGCG CTGCACTACA GTGACGAGGT GGAGATCATC 
CAAGGCCTCA CGCGCATGCC TGGCGGCCGC GACGCACTCA AAAGCAGCGT GGACGCGGTC 
AAGTACTTTG GGAAGGGCAC CTACACCGAC TGCGCTATCA AGAAGGGGCT GGAGCAGCTC 
CTCGTGGGGG GCTCCCACCT GAAGGAGAAT AAGTACCTGA TTGTGGTGAC CGACGGGCAC 
CCCCTGGAGG GCTACAAGGA ACCCTGTGGG GGGCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CACCTGGGCG TCAAAGTCTT CTCGGTGGCC ATCACACCCG ACCACCTGGA GCCGCGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGCCAG 
AGCCGCGACG CAGAGGAGGC CATCAGCCAG ACCATCGACA CCATCGTGGA CATGATCAAA 
AATAACGTGG AGCAAGTGTG CTGCTCCTTC GAATGCCAGC CTGCAAGAGG ACCTCCGGGG 
CTCCGGGGCG ACCCCGGCTT TGAGGGAGAA CGAGGCAAGC CGGGGCTCCC AGGAGAGAAG 
GGAGAAGCCG GAGATCCTGG AAGACCCGGG GACCTCGGAC CTGTTGGGTA CCAGGGAATG 
AAGGGAGAAA AAGGGAGCCG TGGGGAGAAG GGCTCCAGGG GACCCAAGGG CTACAAGGGA 
GAGAAGGGCA AGCGTGGCAT CGACGGGGTG GACGGCGTGA AGGGGGAGAT GGGGTACCCA 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGG TTTGACGGCA TTCAAGGACC CCCTGGCCCC 
AAGGGAGACC CCGGTGCCTT TGGACTGAAA GGAGAAAAGG GCGAGCCTGG AGCTGACGGG 
GAGGCGGGGA GACCAGGGAG CTCGGGACCA TCTGGAGACG AGGGCCAGCC GGGAGAGCCT 
GGGCCCCCCG GAGAGAAAGG AGAGGCGGGC GACGAGGGGA ACCCAGGACC TGACGGTGCC 
CCCGGGGAGC GGGGTGGCCC TGGAGAGAGA GGACCACGGG GGACCCCAGG CACGCGGGGA 
CCAAGAGGAG ACCCTGGTGA AGCTGGCCCG CAGGGTGATC AGGGAAGAGA AGGCCCCGTT 
GGTGTCCCTG GAGACCCGGG CGAGGCTGGC CCTATCGGAC CTAAAGGCTA CCGAGGCGAT 
GAGGGTCCCC CAGGGTCCGA GGGTGCCAGA GGAGCCCCAG GACCTGCCGG ACCCCCTGGA 
GACCCGGGGC TGATGGGTGA AAGGGGAGAA GACGGCCCCG CTGGAAATGG CACCGAGGGC 
TTCCCCGGCT TCCCCGGGTA TCCGGGCAAC AGGGGCGCTC CCGGGATAAA CGGCACGAAG 
GGCTACCCCG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA CGATAACAAC 
GACATTGCAC CCCGAGGAGT CAAAGGAGCA AAGGGGTACC GGGGTCCCGA GGGCCCCCAG 
GGACCCCCAG GACACCAAGG ACCGCCTGGG CCGGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAATGCAAG TGCGGCCCCA TCGACCTCCT GTTCGTGCTG 
GACAGCTCAG AGAGCATTGG CCTGCAGAAC TTCGAGATTG CCAAGGACTT CGTCGTCAAG 
GTCATCGACC GGCTGAGCCG GGACGAGCTG GTCAAGTTCG AGCCAGGGCA GTCGTACGCG 
GGTGTGGTGC AGTACAGCCA CAGCCAGATG CAGGAGCACG TGAGCCTGCG CAGCCCCAGC 
ATCCGGAACG TGCAGGAGCT CAAGGAAGCC ATCAAGAGCC TGCAGTGGAT GGCGGGCGGC 
ACCTTCACGG GGGAGGCCCT GCAGTACACG CGGGACCAGC TGCTGCCGCC CAGCCCGAAC 
AACCGCATCG CCCTGGTCAT CACTGACGGG CGCTCAGACA CTCAGAGGGA CACCACACCG 
CTCAACGTGC TCTGCAGCCC CGGCATCCAG GTGGTCTCCG TGGGCATCAA AGACGTGTTT 
GACTTCATCC CAGGCTCAGA CCAGCTCAAT GTCATTTCTT GCCAAGGCCT GGCACCATCC 
CAGGGCCGGC CCGGCCTCTC GCTGGTCAAG GAGAACTATG CAGAGCTGCT GGAGGATGCC 
TTCCTGAAGA ATGTCACCGC CCAGATCTGC ATAGACAAGA AGTGTCCAGA TTACACCTGC 
CCCATCACGT TCTCCTCCCC GGCTGACATC ACCATCCTGC TGGACGGCTC CGCCAGCGTG 
GGCAGCCACA ACTTTGACAC CACCAAGCGC TTCGCCAAGC GCCTGGCCGA GCGCTTCCTC 
ACAGCGGGCA GGACGGACCC CGCCCACGAC GTGCGGGTGG CGGTGGTGCA GTACAGCGGC 
ACGGGCCAGC AGCGCCCAGA GCGGGCGTCG CTGCAGTTCC TGCAGAACTA CACGGCCCTG 
GCCAGTGCCG TCGATGCCAT GGACTTTATC AACGACGCCA CCGACGTCAA CGATGCCCTG 
GGCTATGTGA CCCGCTTCTA CCGCGAGGCC TCGTCCGGCG CTGCCAAGAA GAGGCTGCTG 
CTCTTCTCAG ATGGCAACTC GCAGGGCGCC ACGCCCGCTG CCATCGAGAA GGCCGTGCAG 
GAAGCCCAGC GGGCAGGCAT CGAGATCTTC GTGGTGGTCG TGGGCCGCCA GGTGAATGAG 
CCCCACATCC GCGTCCTGGT CACCGGCAAG ACGGCCGAGT ACGACGTGGC CTACGGCGAG 
AGCCACCTGT TCCGTGTCCC CAGCTACCAG GCCCTGCTCC GCGGTGTCTT CCACCAGACA 
GTCTCCAGGA AGGTGGCGCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001839.2
CDS	103..3189
Translation	MRAARALLPLLLQACWTAAQDEPETPRAVAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCDRNLVWNAGALHYSDEVEIIQGLTRMPGGRDALKSSVDAVKYFGKGTYTDCAIKKGLEQLLVGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGVKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTIVDMIKNNVEQVCCSFECQPARGPPGLRGDPGFEGERGKPGLPGEKGEAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGIDGVDGVKGEMGYPGLPGCKGSPGFDGIQGPPGPKGDPGAFGLKGEKGEPGADGEAGRPGSSGPSGDEGQPGEPGPPGEKGEAGDEGNPGPDGAPGERGGPGERGPRGTPGTRGPRGDPGEAGPQGDQGREGPVGVPGDPGEAGPIGPKGYRGDEGPPGSEGARGAPGPAGPPGDPGLMGERGEDGPAGNGTEGFPGFPGYPGNRGAPGINGTKGYPGLKGDEGEAGDPGDDNNDIAPRGVKGAKGYRGPEGPQGPPGHQGPPGPDECEILDIIMKMCSCCECKCGPIDLLFVLDSSESIGLQNFEIAKDFVVKVIDRLSRDELVKFEPGQSYAGVVQYSHSQMQEHVSLRSPSIRNVQELKEAIKSLQWMAGGTFTGEALQYTRDQLLPPSPNNRIALVITDGRSDTQRDTTPLNVLCSPGIQVVSVGIKDVFDFIPGSDQLNVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG

Target ORF information:

RefSeq Version	NM_001848.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VI alpha 1 chain (COL6A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001848.2

ORF Insert Sequence:

ATGAGGGCGG CCCGTGCTCT GCTGCCCCTG CTGCTGCAGG CCTGCTGGAC AGCCGCGCAG 
GATGAGCCGG AGACCCCGAG GGCCGTGGCC TTCCAGGACT GCCCCGTGGA CCTGTTCTTT 
GTGCTGGACA CCTCTGAGAG CGTGGCCCTG AGGCTGAAGC CCTACGGGGC CCTCGTGGAC 
AAAGTCAAGT CCTTCACCAA GCGCTTCATC GACAACCTGA GGGACAGGTA CTACCGCTGT 
GACCGAAACC TGGTGTGGAA CGCAGGCGCG CTGCACTACA GTGACGAGGT GGAGATCATC 
CAAGGCCTCA CGCGCATGCC TGGCGGCCGC GACGCACTCA AAAGCAGCGT GGACGCGGTC 
AAGTACTTTG GGAAGGGCAC CTACACCGAC TGCGCTATCA AGAAGGGGCT GGAGCAGCTC 
CTCGTGGGGG GCTCCCACCT GAAGGAGAAT AAGTACCTGA TTGTGGTGAC CGACGGGCAC 
CCCCTGGAGG GCTACAAGGA ACCCTGTGGG GGGCTGGAGG ATGCTGTGAA CGAGGCCAAG 
CACCTGGGCG TCAAAGTCTT CTCGGTGGCC ATCACACCCG ACCACCTGGA GCCGCGTCTG 
AGCATCATCG CCACGGACCA CACGTACCGG CGCAACTTCA CGGCGGCTGA CTGGGGCCAG 
AGCCGCGACG CAGAGGAGGC CATCAGCCAG ACCATCGACA CCATCGTGGA CATGATCAAA 
AATAACGTGG AGCAAGTGTG CTGCTCCTTC GAATGCCAGC CTGCAAGAGG ACCTCCGGGG 
CTCCGGGGCG ACCCCGGCTT TGAGGGAGAA CGAGGCAAGC CGGGGCTCCC AGGAGAGAAG 
GGAGAAGCCG GAGATCCTGG AAGACCCGGG GACCTCGGAC CTGTTGGGTA CCAGGGAATG 
AAGGGAGAAA AAGGGAGCCG TGGGGAGAAG GGCTCCAGGG GACCCAAGGG CTACAAGGGA 
GAGAAGGGCA AGCGTGGCAT CGACGGGGTG GACGGCGTGA AGGGGGAGAT GGGGTACCCA 
GGCCTGCCAG GCTGCAAGGG CTCGCCCGGG TTTGACGGCA TTCAAGGACC CCCTGGCCCC 
AAGGGAGACC CCGGTGCCTT TGGACTGAAA GGAGAAAAGG GCGAGCCTGG AGCTGACGGG 
GAGGCGGGGA GACCAGGGAG CTCGGGACCA TCTGGAGACG AGGGCCAGCC GGGAGAGCCT 
GGGCCCCCCG GAGAGAAAGG AGAGGCGGGC GACGAGGGGA ACCCAGGACC TGACGGTGCC 
CCCGGGGAGC GGGGTGGCCC TGGAGAGAGA GGACCACGGG GGACCCCAGG CACGCGGGGA 
CCAAGAGGAG ACCCTGGTGA AGCTGGCCCG CAGGGTGATC AGGGAAGAGA AGGCCCCGTT 
GGTGTCCCTG GAGACCCGGG CGAGGCTGGC CCTATCGGAC CTAAAGGCTA CCGAGGCGAT 
GAGGGTCCCC CAGGGTCCGA GGGTGCCAGA GGAGCCCCAG GACCTGCCGG ACCCCCTGGA 
GACCCGGGGC TGATGGGTGA AAGGGGAGAA GACGGCCCCG CTGGAAATGG CACCGAGGGC 
TTCCCCGGCT TCCCCGGGTA TCCGGGCAAC AGGGGCGCTC CCGGGATAAA CGGCACGAAG 
GGCTACCCCG GCCTCAAGGG GGACGAGGGA GAAGCCGGGG ACCCCGGAGA CGATAACAAC 
GACATTGCAC CCCGAGGAGT CAAAGGAGCA AAGGGGTACC GGGGTCCCGA GGGCCCCCAG 
GGACCCCCAG GACACCAAGG ACCGCCTGGG CCGGACGAAT GCGAGATTTT GGACATCATC 
ATGAAAATGT GCTCTTGCTG TGAATGCAAG TGCGGCCCCA TCGACCTCCT GTTCGTGCTG 
GACAGCTCAG AGAGCATTGG CCTGCAGAAC TTCGAGATTG CCAAGGACTT CGTCGTCAAG 
GTCATCGACC GGCTGAGCCG GGACGAGCTG GTCAAGTTCG AGCCAGGGCA GTCGTACGCG 
GGTGTGGTGC AGTACAGCCA CAGCCAGATG CAGGAGCACG TGAGCCTGCG CAGCCCCAGC 
ATCCGGAACG TGCAGGAGCT CAAGGAAGCC ATCAAGAGCC TGCAGTGGAT GGCGGGCGGC 
ACCTTCACGG GGGAGGCCCT GCAGTACACG CGGGACCAGC TGCTGCCGCC CAGCCCGAAC 
AACCGCATCG CCCTGGTCAT CACTGACGGG CGCTCAGACA CTCAGAGGGA CACCACACCG 
CTCAACGTGC TCTGCAGCCC CGGCATCCAG GTGGTCTCCG TGGGCATCAA AGACGTGTTT 
GACTTCATCC CAGGCTCAGA CCAGCTCAAT GTCATTTCTT GCCAAGGCCT GGCACCATCC 
CAGGGCCGGC CCGGCCTCTC GCTGGTCAAG GAGAACTATG CAGAGCTGCT GGAGGATGCC 
TTCCTGAAGA ATGTCACCGC CCAGATCTGC ATAGACAAGA AGTGTCCAGA TTACACCTGC 
CCCATCACGT TCTCCTCCCC GGCTGACATC ACCATCCTGC TGGACGGCTC CGCCAGCGTG 
GGCAGCCACA ACTTTGACAC CACCAAGCGC TTCGCCAAGC GCCTGGCCGA GCGCTTCCTC 
ACAGCGGGCA GGACGGACCC CGCCCACGAC GTGCGGGTGG CGGTGGTGCA GTACAGCGGC 
ACGGGCCAGC AGCGCCCAGA GCGGGCGTCG CTGCAGTTCC TGCAGAACTA CACGGCCCTG 
GCCAGTGCCG TCGATGCCAT GGACTTTATC AACGACGCCA CCGACGTCAA CGATGCCCTG 
GGCTATGTGA CCCGCTTCTA CCGCGAGGCC TCGTCCGGCG CTGCCAAGAA GAGGCTGCTG 
CTCTTCTCAG ATGGCAACTC GCAGGGCGCC ACGCCCGCTG CCATCGAGAA GGCCGTGCAG 
GAAGCCCAGC GGGCAGGCAT CGAGATCTTC GTGGTGGTCG TGGGCCGCCA GGTGAATGAG 
CCCCACATCC GCGTCCTGGT CACCGGCAAG ACGGCCGAGT ACGACGTGGC CTACGGCGAG 
AGCCACCTGT TCCGTGTCCC CAGCTACCAG GCCCTGCTCC GCGGTGTCTT CCACCAGACA 
GTCTCCAGGA AGGTGGCGCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL6A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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