DNAJC24 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAJC24
Entrez Gene ID	120526
Full Name	DnaJ heat shock protein family (Hsp40) member C24
Synonyms	DPH4,JJJ3,ZCSL3
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member C24 Names dnaJ homolog subfamily C member 24 1700030A21Rik CSL-type zinc finger-containing protein 3 DPH4 homolog (JJJ3, S. cerevisiae) DPH4, JJJ3 homolog DnaJ (Hsp40) homolog, subfamily C, member 24 diphthamide biosynthesis protein 4 zinc finger, CSL domain containing 3 zinc finger, CSL-type containing 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p13
Summary	Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611072

mRNA and Protein(s)

mRNA	Protein	Name
NM_181706.5	NP_859057.4	dnaJ homolog subfamily C member 24
XM_017017216.1	XP_016872705.1	dnaJ homolog subfamily C member 24 isoform X1
NM_181706.4	NP_859057.4	dnaJ homolog subfamily C member 24

Pathways from BioSystems

Homologies

Homo sapiens (human)	DNAJC24	NP_859057.4
Canis lupus familiaris (dog)	DNAJC24	XP_003432405.1
Mus musculus (house mouse)	Dnajc24	NP_081268.1
Danio rerio (zebrafish)	dnajc24	NP_956746.1
Rattus norvegicus (Norway rat)	Dnajc24	NP_001178782.1
Bos taurus (cattle)	DNAJC24	NP_001071570.1
Pan troglodytes (chimpanzee)	DNAJC24	XP_001141859.1
	DNAJC24	XP_001086365.1
Gallus gallus (chicken)	DNAJC24	NP_001177825.1
Xenopus tropicalis (tropical clawed frog)	dnajc24	NP_001037943.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association and admixture analysis of glaucoma in the Women's Health Initiative.
Hoffmann TJ, Tang H, Thornton TA, Caan B, Haan M, Millen AE, Thomas F, Risch N
Human molecular genetics23(24)6634-43(2014 Dec)

Immunotoxin resistance via reversible methylation of the DPH4 promoter is a unique survival strategy.
Wei H, Xiang L, Wayne AS, Chertov O, FitzGerald DJ, Bera TK, Pastan I
Proceedings of the National Academy of Sciences of the United States of America109(18)6898-903(2012 May)

Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein cochaperone, Dph4.
Thakur A, Chitoor B, Goswami AV, Pareek G, Atreya HS, D'Silva P
The Journal of biological chemistry287(16)13194-205(2012 Apr)

Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.
Liu S, Milne GT, Kuremsky JG, Fink GR, Leppla SH
Molecular and cellular biology24(21)9487-97(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

immunotoxin resistance is associated with reversible CpG island methylation and silencing of DPH4 gene transcription
Title: Immunotoxin resistance via reversible methylation of the DPH4 promoter is a unique survival strategy.

We report here the solution structure and mechanism of novel iron-mediated functional roles of human Dph4, a type III J-protein playing a vital role in diphthamide biosynthesis and normal development.
Title: Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein cochaperone, Dph4.

The following DNAJC24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAJC24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30947	NM_181706.5 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu91947	XM_017017216.1 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu30947	NM_181706.4	Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAJC24 ( NM_181706.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_859057.4 Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), mRNA.

Price & Availability↑

CloneID	OHu30947
Clone ID Related Accession (Same CDS sequence)	NM_181706.5 , NM_181706.4
Accession Version	NM_181706.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily C member 24
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833128.1 and AC108456.7. On Nov 22, 2018 this sequence version replaced NM_181706.4. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other species. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 1 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. ##Evidence-Data-START## Transcript exon combination :: DA659255.1, SRR3476690.655970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000465995.6/ ENSP00000417548.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGGCGG TTGAGCAGAT GCCAAAAAAG GATTGGTACA GCATCCTGGG AGCAGACCCA 
TCTGCAAATA TATCAGACCT AAAACAAAAA TATCAAAAAC TCATATTAAT GTATCATCCA 
GATAAACAAA GTACAGATGT ACCAGCAGGA ACAGTGGAGG AATGTGTACA GAAGTTCATC 
GAAATTGATC AAGCATGGAA AATTCTAGGA AATGAAGAGA CAAAAAGAGA GTATGACCTG 
CAGCGGTGTG AAGATGATCT AAGAAATGTA GGACCAGTAG ATGCTCAAGT ATATCTTGAA 
GAAATGTCTT GGAATGAAGG TGATCACTCT TTTTATCTGA GTTGCAGATG TGGTGGAAAA 
TACAGTGTTT CCAAGGATGA AGCGGAAGAA GTTAGCCTGA TTTCTTGTGA TACATGTTCA 
CTAATTATAG AACTCCTTCA TTATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_859057.4
CDS	87..536
Translation	MMAVEQMPKKDWYSILGADPSANISDLKQKYQKLILMYHPDKQSTDVPAGTVEECVQKFIEIDQAWKILGNEETKREYDLQRCEDDLRNVGPVDAQVYLEEMSWNEGDHSFYLSCRCGGKYSVSKDEAEEVSLISCDTCSLIIELLHYN

Target ORF information:

RefSeq Version	NM_181706.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181706.5

ORF Insert Sequence:

ATGATGGCGG TTGAGCAGAT GCCAAAAAAG GATTGGTACA GCATCCTGGG AGCAGACCCA 
TCTGCAAATA TATCAGACCT AAAACAAAAA TATCAAAAAC TCATATTAAT GTATCATCCA 
GATAAACAAA GTACAGATGT ACCAGCAGGA ACAGTGGAGG AATGTGTACA GAAGTTCATC 
GAAATTGATC AAGCATGGAA AATTCTAGGA AATGAAGAGA CAAAAAGAGA GTATGACCTG 
CAGCGGTGTG AAGATGATCT AAGAAATGTA GGACCAGTAG ATGCTCAAGT ATATCTTGAA 
GAAATGTCTT GGAATGAAGG TGATCACTCT TTTTATCTGA GTTGCAGATG TGGTGGAAAA 
TACAGTGTTT CCAAGGATGA AGCGGAAGAA GTTAGCCTGA TTTCTTGTGA TACATGTTCA 
CTAATTATAG AACTCCTTCA TTATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJC24 ( NM_181706.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_859057.4 Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), mRNA.

Price & Availability↑

CloneID	OHu30947
Clone ID Related Accession (Same CDS sequence)	NM_181706.5 , NM_181706.4
Accession Version	NM_181706.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily C member 24
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD685768.1, AL833128.1 and AC108456.7. On Mar 2, 2006 this sequence version replaced NM_181706.3. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other species. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 1 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.71364.1, SRR1660805.201020.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGATGGCGG TTGAGCAGAT GCCAAAAAAG GATTGGTACA GCATCCTGGG AGCAGACCCA 
TCTGCAAATA TATCAGACCT AAAACAAAAA TATCAAAAAC TCATATTAAT GTATCATCCA 
GATAAACAAA GTACAGATGT ACCAGCAGGA ACAGTGGAGG AATGTGTACA GAAGTTCATC 
GAAATTGATC AAGCATGGAA AATTCTAGGA AATGAAGAGA CAAAAAGAGA GTATGACCTG 
CAGCGGTGTG AAGATGATCT AAGAAATGTA GGACCAGTAG ATGCTCAAGT ATATCTTGAA 
GAAATGTCTT GGAATGAAGG TGATCACTCT TTTTATCTGA GTTGCAGATG TGGTGGAAAA 
TACAGTGTTT CCAAGGATGA AGCGGAAGAA GTTAGCCTGA TTTCTTGTGA TACATGTTCA 
CTAATTATAG AACTCCTTCA TTATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_859057.4
CDS	117..566
Translation	MMAVEQMPKKDWYSILGADPSANISDLKQKYQKLILMYHPDKQSTDVPAGTVEECVQKFIEIDQAWKILGNEETKREYDLQRCEDDLRNVGPVDAQVYLEEMSWNEGDHSFYLSCRCGGKYSVSKDEAEEVSLISCDTCSLIIELLHYN

Target ORF information:

RefSeq Version	NM_181706.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181706.4

ORF Insert Sequence:

ATGATGGCGG TTGAGCAGAT GCCAAAAAAG GATTGGTACA GCATCCTGGG AGCAGACCCA 
TCTGCAAATA TATCAGACCT AAAACAAAAA TATCAAAAAC TCATATTAAT GTATCATCCA 
GATAAACAAA GTACAGATGT ACCAGCAGGA ACAGTGGAGG AATGTGTACA GAAGTTCATC 
GAAATTGATC AAGCATGGAA AATTCTAGGA AATGAAGAGA CAAAAAGAGA GTATGACCTG 
CAGCGGTGTG AAGATGATCT AAGAAATGTA GGACCAGTAG ATGCTCAAGT ATATCTTGAA 
GAAATGTCTT GGAATGAAGG TGATCACTCT TTTTATCTGA GTTGCAGATG TGGTGGAAAA 
TACAGTGTTT CCAAGGATGA AGCGGAAGAA GTTAGCCTGA TTTCTTGTGA TACATGTTCA 
CTAATTATAG AACTCCTTCA TTATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJC24 ( XM_017017216.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872705.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu91947
Clone ID Related Accession (Same CDS sequence)	XM_017017216.1
Accession Version	XM_017017216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily C member 24 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG TTGAGCAGAT GCCAAAAAAG GATTGGTACA GCATCCTGGG AGCAGACCCA 
TCTGCAAATA TATCAGACCT AAAACAAAAA TATCAAAAAC TCATATTAAT GTATCATCCA 
GATAAACAAA GTACAGATGT ACCAGCAGGA ACAGTGGAGG AATGTGTACA GAAGTTCATC 
GAAATTGATC AAGCATGGAA AATTCTAGGA AATGAAGAGA CAAAAAGAGA GTATGACCTG 
CAGCGGTGTG AAGATGATCT AAGAAATGTA GGACCAGTAG ATGCTCAAGT ATATCTTGAA 
GAAATGTCTT GGAATGAAGT TACTTCTCAG AGACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872705.1
CDS	102..440
Translation	MMAVEQMPKKDWYSILGADPSANISDLKQKYQKLILMYHPDKQSTDVPAGTVEECVQKFIEIDQAWKILGNEETKREYDLQRCEDDLRNVGPVDAQVYLEEMSWNEVTSQRQ

Target ORF information:

RefSeq Version	XM_017017216.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member C24 (DNAJC24), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017216.1

ORF Insert Sequence:

ATGATGGCGG TTGAGCAGAT GCCAAAAAAG GATTGGTACA GCATCCTGGG AGCAGACCCA 
TCTGCAAATA TATCAGACCT AAAACAAAAA TATCAAAAAC TCATATTAAT GTATCATCCA 
GATAAACAAA GTACAGATGT ACCAGCAGGA ACAGTGGAGG AATGTGTACA GAAGTTCATC 
GAAATTGATC AAGCATGGAA AATTCTAGGA AATGAAGAGA CAAAAAGAGA GTATGACCTG 
CAGCGGTGTG AAGATGATCT AAGAAATGTA GGACCAGTAG ATGCTCAAGT ATATCTTGAA 
GAAATGTCTT GGAATGAAGT TACTTCTCAG AGACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association and admixture analysis of glaucoma in the Women's Health Initiative.
Human molecular genetics23(24)6634-43(2014 Dec)
Hoffmann TJ,Tang H,Thornton TA,Caan B,Haan M,Millen AE,Thomas F,Risch N

Immunotoxin resistance via reversible methylation of the DPH4 promoter is a unique survival strategy.
Proceedings of the National Academy of Sciences of the United States of America109(18)6898-903(2012 May)
Wei H,Xiang L,Wayne AS,Chertov O,FitzGerald DJ,Bera TK,Pastan I

Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein cochaperone, Dph4.
The Journal of biological chemistry287(16)13194-205(2012 Apr)
Thakur A,Chitoor B,Goswami AV,Pareek G,Atreya HS,D'Silva P

Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.
Molecular and cellular biology24(21)9487-97(2004 Nov)
Liu S,Milne GT,Kuremsky JG,Fink GR,Leppla SH

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DNAJC24 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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