SH2D1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SH2D1B
Entrez Gene ID	117157
Full Name	SH2 domain containing 1B
Synonyms	EAT2
General protein information	Preferred Names SH2 domain containing 1B Names SH2 domain-containing protein 1B EAT-2 EWS/FLI1-activated transcript 2 SH2 domain-containing molecule EAT2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q23.3
Summary	By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008].
Disorder MIM:	608510

mRNA and Protein(s)

mRNA	Protein	Name
NM_053282.4	NP_444512.2	SH2 domain-containing protein 1B
NM_053282.5	NP_444512.2	SH2 domain-containing protein 1B

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH2D1B	NP_444512.2
Pan troglodytes (chimpanzee)	SH2D1B	XP_003949678.1
Rattus norvegicus (Norway rat)	Sh2d1b	XP_577285.1
Canis lupus familiaris (dog)	SH2D1B	XP_005640915.1
Bos taurus (cattle)	SH2D1B	NP_001180091.1
Mus musculus (house mouse)	Sh2d1b1	NP_036139.3
Danio rerio (zebrafish)	si:dkey-11p10.10	NP_001189421.1
Xenopus tropicalis (tropical clawed frog)	LOC100486929	XP_002932331.1
	SH2D1B	XP_001118174.1
Gallus gallus (chicken)	SH2D1B	NP_001265002.1

Gene Ontology
Bibliography

Related articles in PubMed

The X-ray Crystallographic Structure of Human EAT2 (SH2D1B).
Taha M, Nezerwa E, Nam HJ
Protein and peptide letters23(10)862-866(2016)

Phospho-tyrosine dependent protein-protein interaction network.
Grossmann A, Benlasfer N, Birth P, Hegele A, Wachsmuth F, Apelt L, Stelzl U
Molecular systems biology11(3)794(2015 Mar)

Genetic association study of QT interval highlights role for calcium signaling pathways in myocardial repolarization.
Arking DE, Pulit SL, Crotti L, van der Harst P, Munroe PB, Koopmann TT, Sotoodehnia N, Rossin EJ, Morley M, Wang X, Johnson AD, Lundby A, Gudbjartsson DF, Noseworthy PA, Eijgelsheim M, Bradford Y, Tarasov KV, D?rr M, M?ller-Nurasyid M, Lahtinen AM, Nolte IM, Smith AV, Bis JC, Isaacs A, Newhouse SJ, Evans DS, Post WS, Waggott D, Lyytik?inen LP, Hicks AA, Eisele L, Ellinghaus D, Hayward C, Navarro P, Ulivi S, Tanaka T, Tester DJ, Chatel S, Gustafsson S, Kumari M, Morris RW, Naluai ?T, Padmanabhan S, Kluttig A, Strohmer B, Panayiotou AG, Torres M, Knoflach M, Hubacek JA, Slowikowski K, Raychaudhuri S, Kumar RD, Harris TB, Launer LJ, Shuldiner AR, Alonso A, Bader JS, Ehret G, Huang H, Kao WH, Strait JB, Macfarlane PW, Brown M, Caulfield MJ, Samani NJ, Kronenberg F, Willeit J, , , Smith JG, Greiser KH, Meyer Zu Schwabedissen H, Werdan K, Carella M, Zelante L, Heckbert SR, Psaty BM, Rotter JI, Kolcic I, Pola?ek O, Wright AF, Griffin M, Daly MJ, , Arnar DO, H?lm H, Thorsteinsdottir U, , Denny JC, Roden DM, Zuvich RL, Emilsson V, Plump AS, Larson MG, O'Donnell CJ, Yin X, Bobbo M, D'Adamo AP, Iorio A, Sinagra G, Carracedo A, Cummings SR, Nalls MA, Jula A, Kontula KK, Marjamaa A, Oikarinen L, Perola M, Porthan K, Erbel R, Hoffmann P, J?ckel KH, K?lsch H, N?then MM, , den Hoed M, Loos RJ, Thelle DS, Gieger C, Meitinger T, Perz S, Peters A, Prucha H, Sinner MF, Waldenberger M, de Boer RA, Franke L, van der Vleuten PA, Beckmann BM, Martens E, Bardai A, Hofman N, Wilde AA, Behr ER, Dalageorgou C, Giudicessi JR, Medeiros-Domingo A, Barc J, Kyndt F, Probst V, Ghidoni A, Insolia R, Hamilton RM, Scherer SW, Brandimarto J, Margulies K, Moravec CE, del Greco M F, Fuchsberger C, O'Connell JR, Lee WK, Watt GC, Campbell H, Wild SH, El Mokhtari NE, Frey N, Asselbergs FW, Mateo Leach I, Navis G, van den Berg MP, van Veldhuisen DJ, Kellis M, Krijthe BP, Franco OH, Hofman A, Kors JA, Uitterlinden AG, Witteman JC, Kedenko L, Lamina C, Oostra BA, Abecasis GR, Lakatta EG, Mulas A, Orr? M, Schlessinger D, Uda M, Markus MR, V?lker U, Snieder H, Spector TD, ?rnl?v J, Lind L, Sundstr?m J, Syv?nen AC, Kivimaki M, K?h?nen M, Mononen N, Raitakari OT, Viikari JS, Adamkova V, Kiechl S, Brion M, Nicolaides AN, Paulweber B, Haerting J, Dominiczak AF, Nyberg F, Whincup PH, Hingorani AD, Schott JJ, Bezzina CR, Ingelsson E, Ferrucci L, Gasparini P, Wilson JF, Rudan I, Franke A, M?hleisen TW, Pramstaller PP, Lehtim?ki TJ, Paterson AD, Parsa A, Liu Y, van Duijn CM, Siscovick DS, Gudnason V, Jamshidi Y, Salomaa V, Felix SB, Sanna S, Ritchie MD, Stricker BH, Stefansson K, Boyer LA, Cappola TP, Olsen JV, Lage K, Schwartz PJ, K??b S, Chakravarti A, Ackerman MJ, Pfeufer A, de Bakker PI, Newton-Cheh C
Nature genetics46(8)826-36(2014 Aug)

Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.
Leung KK, Hause RJ Jr, Barkinge JL, Ciaccio MF, Chuu CP, Jones RB
Molecular & cellular proteomics : MCP13(7)1705-23(2014 Jul)

EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase C?, Ca++, and Erk, leading to granule polarization.
P?rez-Quintero LA, Roncagalli R, Guo H, Latour S, Davidson D, Veillette A
The Journal of experimental medicine211(4)727-42(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

When compared with SAP, the other single SH2 domain protein in human, EAT2 shows similar binding energies to unphosphorylated ligands. This is inconsistent to the previous data showing low affinity of EAT2 toward unphosphorylated peptides compared to SAP which shows high affinity
Title: The X-ray Crystallographic Structure of Human EAT2 (SH2D1B).

Data indicate that Ewing's sarcoma-associated transcript-2 (EAT-2) over-expression increased the anti-tumor activity of NK cells against K562 tumor cell. targets.
Title: Manipulation of EAT-2 expression promotes induction of multiple beneficial regulatory and effector functions of the human innate immune system as a novel immunomodulatory strategy.

EAT-2 mediates its effects in natural killer cells by linking SLAM family receptors to phospholipase Cgamma, calcium fluxes, and Erk kinase.
Title: EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase C?, Ca++, and Erk, leading to granule polarization.

NTB-A-mediated IFN-gamma production was greatly reduced in the absence of SLAM-associated protein (SAP), demonstrating that cytokine production and cytotoxicity are differentially dependent on SAP and possibly EAT-2
Title: Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells.

The following SH2D1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SH2D1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05651	NM_053282.5 Latest version!	Homo sapiens SH2 domain containing 1B (SH2D1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu05651	NM_053282.4	Homo sapiens SH2 domain containing 1B (SH2D1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SH2D1B ( NM_053282.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_444512.2 Homo sapiens SH2 domain containing 1B (SH2D1B), mRNA.

Price & Availability↑

CloneID	OHu05651
Clone ID Related Accession (Same CDS sequence)	NM_053282.4 , NM_053282.5
Accession Version	NM_053282.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	SH2 domain-containing protein 1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL709120.1, BC022407.1, BC066595.1, AK097162.1 and AF484964.1. On Nov 15, 2004 this sequence version replaced NM_053282.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF484964.1, AK097162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END##

ATGGATCTGC CTTACTACCA TGGACGTCTG ACCAAGCAAG ACTGTGAGAC CTTGCTGCTC 
AAGGAAGGGG TGGATGGCAA CTTTCTTTTA AGAGACAGCG AGTCGATACC AGGAGTCCTG 
TGCCTCTGTG TCTCGTTTAA AAATATTGTC TACACATACC GAATCTTCAG AGAGAAACAC 
GGGTATTACA GGATACAGAC TGCAGAAGGT TCTCCAAAAC AGGTCTTTCC AAGCCTAAAG 
GAACTGATCT CCAAATTTGA AAAACCAAAT CAGGGGATGG TGGTTCACCT TTTAAAGCCA 
ATAAAGAGAA CCAGCCCCAG CTTGAGATGG AGAGGATTGA AATTAGAGTT GGAAACATTT 
GTGAACAGTA ACAGCGATTA TGTGGATGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444512.2
CDS	123..521
Translation	MDLPYYHGRLTKQDCETLLLKEGVDGNFLLRDSESIPGVLCLCVSFKNIVYTYRIFREKHGYYRIQTAEGSPKQVFPSLKELISKFEKPNQGMVVHLLKPIKRTSPSLRWRGLKLELETFVNSNSDYVDVLP

Target ORF information:

RefSeq Version	NM_053282.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SH2 domain containing 1B (SH2D1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053282.4

ORF Insert Sequence:

ATGGATCTGC CTTACTACCA TGGACGTCTG ACCAAGCAAG ACTGTGAGAC CTTGCTGCTC 
AAGGAAGGGG TGGATGGCAA CTTTCTTTTA AGAGACAGCG AGTCGATACC AGGAGTCCTG 
TGCCTCTGTG TCTCGTTTAA AAATATTGTC TACACATACC GAATCTTCAG AGAGAAACAC 
GGGTATTACA GGATACAGAC TGCAGAAGGT TCTCCAAAAC AGGTCTTTCC AAGCCTAAAG 
GAACTGATCT CCAAATTTGA AAAACCAAAT CAGGGGATGG TGGTTCACCT TTTAAAGCCA 
ATAAAGAGAA CCAGCCCCAG CTTGAGATGG AGAGGATTGA AATTAGAGTT GGAAACATTT 
GTGAACAGTA ACAGCGATTA TGTGGATGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH2D1B ( NM_053282.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_444512.2 Homo sapiens SH2 domain containing 1B (SH2D1B), mRNA.

Price & Availability↑

CloneID	OHu05651
Clone ID Related Accession (Same CDS sequence)	NM_053282.4 , NM_053282.5
Accession Version	NM_053282.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	SH2 domain-containing protein 1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL512785.7. On Nov 23, 2018 this sequence version replaced NM_053282.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF484964.1, AK097162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367929.3/ ENSP00000356906.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATCTGC CTTACTACCA TGGACGTCTG ACCAAGCAAG ACTGTGAGAC CTTGCTGCTC 
AAGGAAGGGG TGGATGGCAA CTTTCTTTTA AGAGACAGCG AGTCGATACC AGGAGTCCTG 
TGCCTCTGTG TCTCGTTTAA AAATATTGTC TACACATACC GAATCTTCAG AGAGAAACAC 
GGGTATTACA GGATACAGAC TGCAGAAGGT TCTCCAAAAC AGGTCTTTCC AAGCCTAAAG 
GAACTGATCT CCAAATTTGA AAAACCAAAT CAGGGGATGG TGGTTCACCT TTTAAAGCCA 
ATAAAGAGAA CCAGCCCCAG CTTGAGATGG AGAGGATTGA AATTAGAGTT GGAAACATTT 
GTGAACAGTA ACAGCGATTA TGTGGATGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444512.2
CDS	121..519
Translation	MDLPYYHGRLTKQDCETLLLKEGVDGNFLLRDSESIPGVLCLCVSFKNIVYTYRIFREKHGYYRIQTAEGSPKQVFPSLKELISKFEKPNQGMVVHLLKPIKRTSPSLRWRGLKLELETFVNSNSDYVDVLP

Target ORF information:

RefSeq Version	NM_053282.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SH2 domain containing 1B (SH2D1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053282.5

ORF Insert Sequence:

ATGGATCTGC CTTACTACCA TGGACGTCTG ACCAAGCAAG ACTGTGAGAC CTTGCTGCTC 
AAGGAAGGGG TGGATGGCAA CTTTCTTTTA AGAGACAGCG AGTCGATACC AGGAGTCCTG 
TGCCTCTGTG TCTCGTTTAA AAATATTGTC TACACATACC GAATCTTCAG AGAGAAACAC 
GGGTATTACA GGATACAGAC TGCAGAAGGT TCTCCAAAAC AGGTCTTTCC AAGCCTAAAG 
GAACTGATCT CCAAATTTGA AAAACCAAAT CAGGGGATGG TGGTTCACCT TTTAAAGCCA 
ATAAAGAGAA CCAGCCCCAG CTTGAGATGG AGAGGATTGA AATTAGAGTT GGAAACATTT 
GTGAACAGTA ACAGCGATTA TGTGGATGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The X-ray Crystallographic Structure of Human EAT2 (SH2D1B).
Protein and peptide letters23(10)862-866(2016)
Taha M,Nezerwa E,Nam HJ

Phospho-tyrosine dependent protein-protein interaction network.
Molecular systems biology11(3)794(2015 Mar)
Grossmann A,Benlasfer N,Birth P,Hegele A,Wachsmuth F,Apelt L,Stelzl U

Genetic association study of QT interval highlights role for calcium signaling pathways in myocardial repolarization.
Nature genetics46(8)826-36(2014 Aug)
Arking DE,Pulit SL,Crotti L,van der Harst P,Munroe PB,Koopmann TT,Sotoodehnia N,Rossin EJ,Morley M,Wang X,Johnson AD,Lundby A,Gudbjartsson DF,Noseworthy PA,Eijgelsheim M,Bradford Y,Tarasov KV,D?rr M,M?ller-Nurasyid M,Lahtinen AM,Nolte IM,Smith AV,Bis JC,Isaacs A,Newhouse SJ,Evans DS,Post WS,Waggott D,Lyytik?inen LP,Hicks AA,Eisele L,Ellinghaus D,Hayward C,Navarro P,Ulivi S,Tanaka T,Tester DJ,Chatel S,Gustafsson S,Kumari M,Morris RW,Naluai ?T,Padmanabhan S,Kluttig A,Strohmer B,Panayiotou AG,Torres M,Knoflach M,Hubacek JA,Slowikowski K,Raychaudhuri S,Kumar RD,Harris TB,Launer LJ,Shuldiner AR,Alonso A,Bader JS,Ehret G,Huang H,Kao WH,Strait JB,Macfarlane PW,Brown M,Caulfield MJ,Samani NJ,Kronenberg F,Willeit J,,,Smith JG,Greiser KH,Meyer Zu Schwabedissen H,Werdan K,Carella M,Zelante L,Heckbert SR,Psaty BM,Rotter JI,Kolcic I,Pola?ek O,Wright AF,Griffin M,Daly MJ,,Arnar DO,H?lm H,Thorsteinsdottir U,,Denny JC,Roden DM,Zuvich RL,Emilsson V,Plump AS,Larson MG,O'Donnell CJ,Yin X,Bobbo M,D'Adamo AP,Iorio A,Sinagra G,Carracedo A,Cummings SR,Nalls MA,Jula A,Kontula KK,Marjamaa A,Oikarinen L,Perola M,Porthan K,Erbel R,Hoffmann P,J?ckel KH,K?lsch H,N?then MM,,den Hoed M,Loos RJ,Thelle DS,Gieger C,Meitinger T,Perz S,Peters A,Prucha H,Sinner MF,Waldenberger M,de Boer RA,Franke L,van der Vleuten PA,Beckmann BM,Martens E,Bardai A,Hofman N,Wilde AA,Behr ER,Dalageorgou C,Giudicessi JR,Medeiros-Domingo A,Barc J,Kyndt F,Probst V,Ghidoni A,Insolia R,Hamilton RM,Scherer SW,Brandimarto J,Margulies K,Moravec CE,del Greco M F,Fuchsberger C,O'Connell JR,Lee WK,Watt GC,Campbell H,Wild SH,El Mokhtari NE,Frey N,Asselbergs FW,Mateo Leach I,Navis G,van den Berg MP,van Veldhuisen DJ,Kellis M,Krijthe BP,Franco OH,Hofman A,Kors JA,Uitterlinden AG,Witteman JC,Kedenko L,Lamina C,Oostra BA,Abecasis GR,Lakatta EG,Mulas A,Orr? M,Schlessinger D,Uda M,Markus MR,V?lker U,Snieder H,Spector TD,?rnl?v J,Lind L,Sundstr?m J,Syv?nen AC,Kivimaki M,K?h?nen M,Mononen N,Raitakari OT,Viikari JS,Adamkova V,Kiechl S,Brion M,Nicolaides AN,Paulweber B,Haerting J,Dominiczak AF,Nyberg F,Whincup PH,Hingorani AD,Schott JJ,Bezzina CR,Ingelsson E,Ferrucci L,Gasparini P,Wilson JF,Rudan I,Franke A,M?hleisen TW,Pramstaller PP,Lehtim?ki TJ,Paterson AD,Parsa A,Liu Y,van Duijn CM,Siscovick DS,Gudnason V,Jamshidi Y,Salomaa V,Felix SB,Sanna S,Ritchie MD,Stricker BH,Stefansson K,Boyer LA,Cappola TP,Olsen JV,Lage K,Schwartz PJ,K??b S,Chakravarti A,Ackerman MJ,Pfeufer A,de Bakker PI,Newton-Cheh C

Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.
Molecular & cellular proteomics : MCP13(7)1705-23(2014 Jul)
Leung KK,Hause RJ Jr,Barkinge JL,Ciaccio MF,Chuu CP,Jones RB

EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase C?, Ca++, and Erk, leading to granule polarization.
The Journal of experimental medicine211(4)727-42(2014 Apr)
P?rez-Quintero LA,Roncagalli R,Guo H,Latour S,Davidson D,Veillette A