ELFN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ELFN2
Entrez Gene ID	114794
Full Name	extracellular leucine rich repeat and fibronectin type III domain containing 2
Synonyms	LRRC62,PPP1R29
General protein information	Preferred Names extracellular leucine rich repeat and fibronectin type III domain containing 2 Names protein phosphatase 1 regulatory subunit 29 dJ63G5.3 (putative Leucine rich protein) leucine-rich repeat and fibronectin type-III domain-containing protein 6 leucine-rich repeat-containing protein 62
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_052906.5	NP_443138.2	protein phosphatase 1 regulatory subunit 29 precursor
NM_052906.4	NP_443138.2	protein phosphatase 1 regulatory subunit 29 precursor

Pathways from BioSystems

Homologies

	ELFN2	XP_001086834.1
Canis lupus familiaris (dog)	ELFN2	XP_005625962.1
Danio rerio (zebrafish)	LOC796777	XP_005164143.1
Danio rerio (zebrafish)	elfn2a	XP_001922657.1
Homo sapiens (human)	ELFN2	NP_443138.2
Pan troglodytes (chimpanzee)	ELFN2	XP_003317295.1
Gallus gallus (chicken)	ELFN2	XP_004937806.1
Xenopus tropicalis (tropical clawed frog)	elfn2	NP_001123827.1
Bos taurus (cattle)	ELFN2	XP_005206934.1
Mus musculus (house mouse)	Elfn2	NP_898964.2
Rattus norvegicus (Norway rat)	Elfn2-ps1	XP_006242103.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
Cobb J, Cule E, Moncrieffe H, Hinks A, Ursu S, Patrick F, Kassoumeri L, Flynn E, Bulatovi? M, Wulffraat N, van Zelst B, de Jonge R, Bohm M, Dolezalova P, Hirani S, Newman S, Whitworth P, Southwood TR, De Iorio M, , , , Wedderburn LR, Thomson W
The pharmacogenomics journal14(4)356-64(2014 Aug)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Hendrickx A, Beullens M, Ceulemans H, Den Abt T, Van Eynde A, Nicolaescu E, Lesage B, Bollen M
Chemistry & biology16(4)365-71(2009 Apr)

The extracellular leucine-rich repeat superfamily; a comparative survey and analysis of evolutionary relationships and expression patterns.
Dolan J, Walshe K, Alsbury S, Hokamp K, O'Keeffe S, Okafuji T, Miller SF, Tear G, Mitchell KJ
BMC genomics8320(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ELFN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ELFN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu53388	NM_052906.5 Latest version!	Homo sapiens extracellular leucine rich repeat and fibronectin type III domain containing 2 (ELFN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$414.50-$580.30 $829.00
View Old Accession Versions >>
OHu53388	NM_052906.4	Homo sapiens extracellular leucine rich repeat and fibronectin type III domain containing 2 (ELFN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$414.50-$580.30 $829.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ELFN2 ( NM_052906.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_443138.2 Homo sapiens extracellular leucine rich repeat and fibronectin type III domain containing 2 (ELFN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu53388
Clone ID Related Accession (Same CDS sequence)	NM_052906.5 , NM_052906.4
Accession Version	NM_052906.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 29 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP325335.1, DA398675.1, Z94160.1, AB067491.1 and BC032082.2. On Nov 22, 2018 this sequence version replaced NM_052906.4. Transcript Variant: This variant (1) represents the longer trancript and encodes the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: DA398675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000402918.7/ ENSP00000385277.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCGCC TGGGGCTGTG CGCGGCGGCG CTGCTGTGCG TGTGCCGGCC GGGTGCCGTG 
CGTGCCGACT GCTGGCTCAT TGAGGGCGAC AAGGGCTACG TGTGGCTGGC CATCTGCAGC 
CAGAACCAGC CGCCCTACGA GACCATCCCG CAGCACATCA ATAGCACCGT GCACGACCTG 
CGGCTCAACG AGAACAAGCT CAAAGCCGTG CTCTACTCCT CGCTCAACCG CTTTGGGAAC 
CTCACCGACC TCAACCTCAC CAAGAACGAG ATCTCCTACA TCGAGGACGG TGCCTTCCTG 
GGCCAGTCGA GCCTGCAGGT CCTGCAGCTG GGCTACAACA AGCTCAGCAA CCTGACGGAG 
GGCATGCTGC GAGGCATGAG CCGCCTGCAG TTCCTCTTTG TCCAGCACAA CCTCATCGAG 
GTGGTGACGC CCACCGCCTT CTCCGAGTGC CCGAGCCTCA TCAGCATCGA CCTGTCCTCC 
AACCGCCTCA GCCGCCTGGA CGGTGCCACC TTTGCCAGCC TCGCCAGCCT GATGGTGTGT 
GAGCTGGCCG GCAACCCCTT CAACTGTGAG TGCGACCTCT TCGGCTTCCT GGCCTGGCTG 
GTGGTCTTCA ACAACGTCAC CAAGAACTAC GACCGCCTGC AGTGTGAGTC GCCGCGGGAG 
TTTGCCGGCT ACCCGCTGCT GGTGCCCCGG CCCTACCACA GCCTCAACGC CATCACCGTA 
CTCCAGGCCA AGTGTCGGAA TGGCTCGCTG CCCGCCCGGC CCGTGAGCCA CCCCACGCCC 
TACTCCACCG ACGCCCAGAG GGAGCCAGAC GAGAACTCGG GCTTCAACCC CGACGAGATC 
CTTTCGGTGG AGCCGCCGGC CTCGTCCACC ACGGATGCGT CGGCAGGGCC AGCCATCAAG 
CTGCACCACG TCACGTTCAC CTCGGCCACC CTGGTGGTCA TCATCCCACA CCCCTACAGC 
AAGATGTACA TCCTCGTGCA GTACAACAAC AGCTACTTCT CCGACGTCAT GACCCTCAAG 
AACAAGAAGG AGATCGTGAC GCTGGACAAA CTGCGGGCGC ACACTGAGTA CACCTTCTGC 
GTGACCTCGC TGCGCAACAG CCGCCGCTTC AACCACACCT GCCTGACCTT CACCACGCGG 
GACCCCGTCC CCGGAGACTT GGCGCCCAGC ACCTCCACCA CCACCCACTA CATCATGACC 
ATCCTGGGCT GCCTCTTTGG CATGGTTATC GTGCTGGGAG CCGTGTACTA CTGCCTGCGC 
AAGCGGCGCA TGCAGGAGGA GAAGCAGAAG TCTGTCAACG TCAAGAAGAC CATCCTGGAG 
ATGCGCTACG GGGCTGATGT GGATGCCGGC TCCATTGTGC ACGCCGCCCA GAAGCTGGGC 
GAGCCTCCCG TGCTGCCCGT ATCTCGCATG GCCTCCATCC CCTCCATGAT CGGGGAGAAG 
CTGCCCACCG CCAAGGGGTT GGAGGCCGGG CTGGACACAC CCAAGGTAGC CACCAAAGGC 
AACTATATCG AGGTGCGCAC AGGCGCCGGC GGGGACGGTC TGGCTCGGCC CGAGGATGAC 
CTCCCGGACC TCGAGAACGG CCAGGGCTCG GCTGCAGAGA TCTCCACCAT TGCCAAGGAG 
GTGGACAAGG TCAACCAGAT CATTAACAAC TGCATCGATG CTCTCAAGCT GGACTCGGCC 
TCTTTTCTGG GAGGCGGCAG CAGCAGTGGG GACCCCGAGC TGGCCTTCGA GTGCCAGTCC 
CTCCCTGCAG CTGCTGCCGC CTCCTCAGCC ACTGGCCCCG GGGCCCTGGA GCGGCCCAGC 
TTCCTTTCGC CTCCCTACAA GGAGAGCTCC CACCACCCAC TACAGCGCCA GCTGAGCGCC 
GACGCGGCCG TGACCCGCAA GACCTGCAGC GTGTCGTCCA GTGGTTCCAT CAAGAGCGCC 
AAGGTCTTTA GCCTGGACGT GCCCGACCAT CCGGCCGCCA CAGGGCTGGC TAAGGGCGAC 
TCCAAGTACA TCGAGAAGGG CAGCCCCCTC AACAGCCCGC TGGACCGGCT CCCGCTGGTG 
CCGGCGGGCA GCGGCGGGGG CAGCGGCGGG GGCGGGGGCA TCCACCACCT GGAGGTGAAG 
CCGGCCTACC ACTGCAGCGA GCACCGGCAC AGCTTTCCCG CCCTGTACTA CGAGGAGGGT 
GCCGACAGCC TGAGCCAGCG CGTGTCCTTC CTCAAGCCGC TGACCCGCTC CAAGCGTGAC 
TCCACCTACT CGCAGCTCTC CCCCAGACAC TACTACTCAG GGTACTCCTC CAGCCCCGAG 
TACTCATCCG AGAGCACGCA CAAGATCTGG GAGCGCTTCC GGCCCTACAA GAAGCACCAC 
CGGGAGGAGG TGTACATGGC CGCCGGTCAC GCCCTGCGCA AGAAGGTCCA GTTCGCCAAG 
GACGAGGATC TGCATGACAT CCTTGATTAC TGGAAGGGGG TCTCCGCCCA GCAGAAGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443138.2
CDS	796..3258
Translation	MLRLGLCAAALLCVCRPGAVRADCWLIEGDKGYVWLAICSQNQPPYETIPQHINSTVHDLRLNENKLKAVLYSSLNRFGNLTDLNLTKNEISYIEDGAFLGQSSLQVLQLGYNKLSNLTEGMLRGMSRLQFLFVQHNLIEVVTPTAFSECPSLISIDLSSNRLSRLDGATFASLASLMVCELAGNPFNCECDLFGFLAWLVVFNNVTKNYDRLQCESPREFAGYPLLVPRPYHSLNAITVLQAKCRNGSLPARPVSHPTPYSTDAQREPDENSGFNPDEILSVEPPASSTTDASAGPAIKLHHVTFTSATLVVIIPHPYSKMYILVQYNNSYFSDVMTLKNKKEIVTLDKLRAHTEYTFCVTSLRNSRRFNHTCLTFTTRDPVPGDLAPSTSTTTHYIMTILGCLFGMVIVLGAVYYCLRKRRMQEEKQKSVNVKKTILEMRYGADVDAGSIVHAAQKLGEPPVLPVSRMASIPSMIGEKLPTAKGLEAGLDTPKVATKGNYIEVRTGAGGDGLARPEDDLPDLENGQGSAAEISTIAKEVDKVNQIINNCIDALKLDSASFLGGGSSSGDPELAFECQSLPAAAAASSATGPGALERPSFLSPPYKESSHHPLQRQLSADAAVTRKTCSVSSSGSIKSAKVFSLDVPDHPAATGLAKGDSKYIEKGSPLNSPLDRLPLVPAGSGGGSGGGGGIHHLEVKPAYHCSEHRHSFPALYYEEGADSLSQRVSFLKPLTRSKRDSTYSQLSPRHYYSGYSSSPEYSSESTHKIWERFRPYKKHHREEVYMAAGHALRKKVQFAKDEDLHDILDYWKGVSAQQKL

Target ORF information:

RefSeq Version	NM_052906.5
Organism	Homo sapiens(human)
Definition	Homo sapiens extracellular leucine rich repeat and fibronectin type III domain containing 2 (ELFN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052906.5

ORF Insert Sequence:

ATGCTGCGCC TGGGGCTGTG CGCGGCGGCG CTGCTGTGCG TGTGCCGGCC GGGTGCCGTG 
CGTGCCGACT GCTGGCTCAT TGAGGGCGAC AAGGGCTACG TGTGGCTGGC CATCTGCAGC 
CAGAACCAGC CGCCCTACGA GACCATCCCG CAGCACATCA ATAGCACCGT GCACGACCTG 
CGGCTCAACG AGAACAAGCT CAAAGCCGTG CTCTACTCCT CGCTCAACCG CTTTGGGAAC 
CTCACCGACC TCAACCTCAC CAAGAACGAG ATCTCCTACA TCGAGGACGG TGCCTTCCTG 
GGCCAGTCGA GCCTGCAGGT CCTGCAGCTG GGCTACAACA AGCTCAGCAA CCTGACGGAG 
GGCATGCTGC GAGGCATGAG CCGCCTGCAG TTCCTCTTTG TCCAGCACAA CCTCATCGAG 
GTGGTGACGC CCACCGCCTT CTCCGAGTGC CCGAGCCTCA TCAGCATCGA CCTGTCCTCC 
AACCGCCTCA GCCGCCTGGA CGGTGCCACC TTTGCCAGCC TCGCCAGCCT GATGGTGTGT 
GAGCTGGCCG GCAACCCCTT CAACTGTGAG TGCGACCTCT TCGGCTTCCT GGCCTGGCTG 
GTGGTCTTCA ACAACGTCAC CAAGAACTAC GACCGCCTGC AGTGTGAGTC GCCGCGGGAG 
TTTGCCGGCT ACCCGCTGCT GGTGCCCCGG CCCTACCACA GCCTCAACGC CATCACCGTA 
CTCCAGGCCA AGTGTCGGAA TGGCTCGCTG CCCGCCCGGC CCGTGAGCCA CCCCACGCCC 
TACTCCACCG ACGCCCAGAG GGAGCCAGAC GAGAACTCGG GCTTCAACCC CGACGAGATC 
CTTTCGGTGG AGCCGCCGGC CTCGTCCACC ACGGATGCGT CGGCAGGGCC AGCCATCAAG 
CTGCACCACG TCACGTTCAC CTCGGCCACC CTGGTGGTCA TCATCCCACA CCCCTACAGC 
AAGATGTACA TCCTCGTGCA GTACAACAAC AGCTACTTCT CCGACGTCAT GACCCTCAAG 
AACAAGAAGG AGATCGTGAC GCTGGACAAA CTGCGGGCGC ACACTGAGTA CACCTTCTGC 
GTGACCTCGC TGCGCAACAG CCGCCGCTTC AACCACACCT GCCTGACCTT CACCACGCGG 
GACCCCGTCC CCGGAGACTT GGCGCCCAGC ACCTCCACCA CCACCCACTA CATCATGACC 
ATCCTGGGCT GCCTCTTTGG CATGGTTATC GTGCTGGGAG CCGTGTACTA CTGCCTGCGC 
AAGCGGCGCA TGCAGGAGGA GAAGCAGAAG TCTGTCAACG TCAAGAAGAC CATCCTGGAG 
ATGCGCTACG GGGCTGATGT GGATGCCGGC TCCATTGTGC ACGCCGCCCA GAAGCTGGGC 
GAGCCTCCCG TGCTGCCCGT ATCTCGCATG GCCTCCATCC CCTCCATGAT CGGGGAGAAG 
CTGCCCACCG CCAAGGGGTT GGAGGCCGGG CTGGACACAC CCAAGGTAGC CACCAAAGGC 
AACTATATCG AGGTGCGCAC AGGCGCCGGC GGGGACGGTC TGGCTCGGCC CGAGGATGAC 
CTCCCGGACC TCGAGAACGG CCAGGGCTCG GCTGCAGAGA TCTCCACCAT TGCCAAGGAG 
GTGGACAAGG TCAACCAGAT CATTAACAAC TGCATCGATG CTCTCAAGCT GGACTCGGCC 
TCTTTTCTGG GAGGCGGCAG CAGCAGTGGG GACCCCGAGC TGGCCTTCGA GTGCCAGTCC 
CTCCCTGCAG CTGCTGCCGC CTCCTCAGCC ACTGGCCCCG GGGCCCTGGA GCGGCCCAGC 
TTCCTTTCGC CTCCCTACAA GGAGAGCTCC CACCACCCAC TACAGCGCCA GCTGAGCGCC 
GACGCGGCCG TGACCCGCAA GACCTGCAGC GTGTCGTCCA GTGGTTCCAT CAAGAGCGCC 
AAGGTCTTTA GCCTGGACGT GCCCGACCAT CCGGCCGCCA CAGGGCTGGC TAAGGGCGAC 
TCCAAGTACA TCGAGAAGGG CAGCCCCCTC AACAGCCCGC TGGACCGGCT CCCGCTGGTG 
CCGGCGGGCA GCGGCGGGGG CAGCGGCGGG GGCGGGGGCA TCCACCACCT GGAGGTGAAG 
CCGGCCTACC ACTGCAGCGA GCACCGGCAC AGCTTTCCCG CCCTGTACTA CGAGGAGGGT 
GCCGACAGCC TGAGCCAGCG CGTGTCCTTC CTCAAGCCGC TGACCCGCTC CAAGCGTGAC 
TCCACCTACT CGCAGCTCTC CCCCAGACAC TACTACTCAG GGTACTCCTC CAGCCCCGAG 
TACTCATCCG AGAGCACGCA CAAGATCTGG GAGCGCTTCC GGCCCTACAA GAAGCACCAC 
CGGGAGGAGG TGTACATGGC CGCCGGTCAC GCCCTGCGCA AGAAGGTCCA GTTCGCCAAG 
GACGAGGATC TGCATGACAT CCTTGATTAC TGGAAGGGGG TCTCCGCCCA GCAGAAGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELFN2 ( NM_052906.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_443138.2 Homo sapiens extracellular leucine rich repeat and fibronectin type III domain containing 2 (ELFN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu53388
Clone ID Related Accession (Same CDS sequence)	NM_052906.5 , NM_052906.4
Accession Version	NM_052906.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 29 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA398675.1, Z94160.1, AB067491.1 and BC032082.2. On Jan 17, 2014 this sequence version replaced NM_052906.3. Transcript Variant: This variant (1) represents the longer trancript and encodes the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: DA398675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGCC TGGGGCTGTG CGCGGCGGCG CTGCTGTGCG TGTGCCGGCC GGGTGCCGTG 
CGTGCCGACT GCTGGCTCAT TGAGGGCGAC AAGGGCTACG TGTGGCTGGC CATCTGCAGC 
CAGAACCAGC CGCCCTACGA GACCATCCCG CAGCACATCA ATAGCACCGT GCACGACCTG 
CGGCTCAACG AGAACAAGCT CAAAGCCGTG CTCTACTCCT CGCTCAACCG CTTTGGGAAC 
CTCACCGACC TCAACCTCAC CAAGAACGAG ATCTCCTACA TCGAGGACGG TGCCTTCCTG 
GGCCAGTCGA GCCTGCAGGT CCTGCAGCTG GGCTACAACA AGCTCAGCAA CCTGACGGAG 
GGCATGCTGC GAGGCATGAG CCGCCTGCAG TTCCTCTTTG TCCAGCACAA CCTCATCGAG 
GTGGTGACGC CCACCGCCTT CTCCGAGTGC CCGAGCCTCA TCAGCATCGA CCTGTCCTCC 
AACCGCCTCA GCCGCCTGGA CGGTGCCACC TTTGCCAGCC TCGCCAGCCT GATGGTGTGT 
GAGCTGGCCG GCAACCCCTT CAACTGTGAG TGCGACCTCT TCGGCTTCCT GGCCTGGCTG 
GTGGTCTTCA ACAACGTCAC CAAGAACTAC GACCGCCTGC AGTGTGAGTC GCCGCGGGAG 
TTTGCCGGCT ACCCGCTGCT GGTGCCCCGG CCCTACCACA GCCTCAACGC CATCACCGTA 
CTCCAGGCCA AGTGTCGGAA TGGCTCGCTG CCCGCCCGGC CCGTGAGCCA CCCCACGCCC 
TACTCCACCG ACGCCCAGAG GGAGCCAGAC GAGAACTCGG GCTTCAACCC CGACGAGATC 
CTTTCGGTGG AGCCGCCGGC CTCGTCCACC ACGGATGCGT CGGCAGGGCC AGCCATCAAG 
CTGCACCACG TCACGTTCAC CTCGGCCACC CTGGTGGTCA TCATCCCACA CCCCTACAGC 
AAGATGTACA TCCTCGTGCA GTACAACAAC AGCTACTTCT CCGACGTCAT GACCCTCAAG 
AACAAGAAGG AGATCGTGAC GCTGGACAAA CTGCGGGCGC ACACTGAGTA CACCTTCTGC 
GTGACCTCGC TGCGCAACAG CCGCCGCTTC AACCACACCT GCCTGACCTT CACCACGCGG 
GACCCCGTCC CCGGAGACTT GGCGCCCAGC ACCTCCACCA CCACCCACTA CATCATGACC 
ATCCTGGGCT GCCTCTTTGG CATGGTTATC GTGCTGGGAG CCGTGTACTA CTGCCTGCGC 
AAGCGGCGCA TGCAGGAGGA GAAGCAGAAG TCTGTCAACG TCAAGAAGAC CATCCTGGAG 
ATGCGCTACG GGGCTGATGT GGATGCCGGC TCCATTGTGC ACGCCGCCCA GAAGCTGGGC 
GAGCCTCCCG TGCTGCCCGT ATCTCGCATG GCCTCCATCC CCTCCATGAT CGGGGAGAAG 
CTGCCCACCG CCAAGGGGTT GGAGGCCGGG CTGGACACAC CCAAGGTAGC CACCAAAGGC 
AACTATATCG AGGTGCGCAC AGGCGCCGGC GGGGACGGTC TGGCTCGGCC CGAGGATGAC 
CTCCCGGACC TCGAGAACGG CCAGGGCTCG GCTGCAGAGA TCTCCACCAT TGCCAAGGAG 
GTGGACAAGG TCAACCAGAT CATTAACAAC TGCATCGATG CTCTCAAGCT GGACTCGGCC 
TCTTTTCTGG GAGGCGGCAG CAGCAGTGGG GACCCCGAGC TGGCCTTCGA GTGCCAGTCC 
CTCCCTGCAG CTGCTGCCGC CTCCTCAGCC ACTGGCCCCG GGGCCCTGGA GCGGCCCAGC 
TTCCTTTCGC CTCCCTACAA GGAGAGCTCC CACCACCCAC TACAGCGCCA GCTGAGCGCC 
GACGCGGCCG TGACCCGCAA GACCTGCAGC GTGTCGTCCA GTGGTTCCAT CAAGAGCGCC 
AAGGTCTTTA GCCTGGACGT GCCCGACCAT CCGGCCGCCA CAGGGCTGGC TAAGGGCGAC 
TCCAAGTACA TCGAGAAGGG CAGCCCCCTC AACAGCCCGC TGGACCGGCT CCCGCTGGTG 
CCGGCGGGCA GCGGCGGGGG CAGCGGCGGG GGCGGGGGCA TCCACCACCT GGAGGTGAAG 
CCGGCCTACC ACTGCAGCGA GCACCGGCAC AGCTTTCCCG CCCTGTACTA CGAGGAGGGT 
GCCGACAGCC TGAGCCAGCG CGTGTCCTTC CTCAAGCCGC TGACCCGCTC CAAGCGTGAC 
TCCACCTACT CGCAGCTCTC CCCCAGACAC TACTACTCAG GGTACTCCTC CAGCCCCGAG 
TACTCATCCG AGAGCACGCA CAAGATCTGG GAGCGCTTCC GGCCCTACAA GAAGCACCAC 
CGGGAGGAGG TGTACATGGC CGCCGGTCAC GCCCTGCGCA AGAAGGTCCA GTTCGCCAAG 
GACGAGGATC TGCATGACAT CCTTGATTAC TGGAAGGGGG TCTCCGCCCA GCAGAAGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443138.2
CDS	787..3249
Translation	MLRLGLCAAALLCVCRPGAVRADCWLIEGDKGYVWLAICSQNQPPYETIPQHINSTVHDLRLNENKLKAVLYSSLNRFGNLTDLNLTKNEISYIEDGAFLGQSSLQVLQLGYNKLSNLTEGMLRGMSRLQFLFVQHNLIEVVTPTAFSECPSLISIDLSSNRLSRLDGATFASLASLMVCELAGNPFNCECDLFGFLAWLVVFNNVTKNYDRLQCESPREFAGYPLLVPRPYHSLNAITVLQAKCRNGSLPARPVSHPTPYSTDAQREPDENSGFNPDEILSVEPPASSTTDASAGPAIKLHHVTFTSATLVVIIPHPYSKMYILVQYNNSYFSDVMTLKNKKEIVTLDKLRAHTEYTFCVTSLRNSRRFNHTCLTFTTRDPVPGDLAPSTSTTTHYIMTILGCLFGMVIVLGAVYYCLRKRRMQEEKQKSVNVKKTILEMRYGADVDAGSIVHAAQKLGEPPVLPVSRMASIPSMIGEKLPTAKGLEAGLDTPKVATKGNYIEVRTGAGGDGLARPEDDLPDLENGQGSAAEISTIAKEVDKVNQIINNCIDALKLDSASFLGGGSSSGDPELAFECQSLPAAAAASSATGPGALERPSFLSPPYKESSHHPLQRQLSADAAVTRKTCSVSSSGSIKSAKVFSLDVPDHPAATGLAKGDSKYIEKGSPLNSPLDRLPLVPAGSGGGSGGGGGIHHLEVKPAYHCSEHRHSFPALYYEEGADSLSQRVSFLKPLTRSKRDSTYSQLSPRHYYSGYSSSPEYSSESTHKIWERFRPYKKHHREEVYMAAGHALRKKVQFAKDEDLHDILDYWKGVSAQQKL

Target ORF information:

RefSeq Version	NM_052906.4
Organism	Homo sapiens(human)
Definition	Homo sapiens extracellular leucine rich repeat and fibronectin type III domain containing 2 (ELFN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052906.4

ORF Insert Sequence:

ATGCTGCGCC TGGGGCTGTG CGCGGCGGCG CTGCTGTGCG TGTGCCGGCC GGGTGCCGTG 
CGTGCCGACT GCTGGCTCAT TGAGGGCGAC AAGGGCTACG TGTGGCTGGC CATCTGCAGC 
CAGAACCAGC CGCCCTACGA GACCATCCCG CAGCACATCA ATAGCACCGT GCACGACCTG 
CGGCTCAACG AGAACAAGCT CAAAGCCGTG CTCTACTCCT CGCTCAACCG CTTTGGGAAC 
CTCACCGACC TCAACCTCAC CAAGAACGAG ATCTCCTACA TCGAGGACGG TGCCTTCCTG 
GGCCAGTCGA GCCTGCAGGT CCTGCAGCTG GGCTACAACA AGCTCAGCAA CCTGACGGAG 
GGCATGCTGC GAGGCATGAG CCGCCTGCAG TTCCTCTTTG TCCAGCACAA CCTCATCGAG 
GTGGTGACGC CCACCGCCTT CTCCGAGTGC CCGAGCCTCA TCAGCATCGA CCTGTCCTCC 
AACCGCCTCA GCCGCCTGGA CGGTGCCACC TTTGCCAGCC TCGCCAGCCT GATGGTGTGT 
GAGCTGGCCG GCAACCCCTT CAACTGTGAG TGCGACCTCT TCGGCTTCCT GGCCTGGCTG 
GTGGTCTTCA ACAACGTCAC CAAGAACTAC GACCGCCTGC AGTGTGAGTC GCCGCGGGAG 
TTTGCCGGCT ACCCGCTGCT GGTGCCCCGG CCCTACCACA GCCTCAACGC CATCACCGTA 
CTCCAGGCCA AGTGTCGGAA TGGCTCGCTG CCCGCCCGGC CCGTGAGCCA CCCCACGCCC 
TACTCCACCG ACGCCCAGAG GGAGCCAGAC GAGAACTCGG GCTTCAACCC CGACGAGATC 
CTTTCGGTGG AGCCGCCGGC CTCGTCCACC ACGGATGCGT CGGCAGGGCC AGCCATCAAG 
CTGCACCACG TCACGTTCAC CTCGGCCACC CTGGTGGTCA TCATCCCACA CCCCTACAGC 
AAGATGTACA TCCTCGTGCA GTACAACAAC AGCTACTTCT CCGACGTCAT GACCCTCAAG 
AACAAGAAGG AGATCGTGAC GCTGGACAAA CTGCGGGCGC ACACTGAGTA CACCTTCTGC 
GTGACCTCGC TGCGCAACAG CCGCCGCTTC AACCACACCT GCCTGACCTT CACCACGCGG 
GACCCCGTCC CCGGAGACTT GGCGCCCAGC ACCTCCACCA CCACCCACTA CATCATGACC 
ATCCTGGGCT GCCTCTTTGG CATGGTTATC GTGCTGGGAG CCGTGTACTA CTGCCTGCGC 
AAGCGGCGCA TGCAGGAGGA GAAGCAGAAG TCTGTCAACG TCAAGAAGAC CATCCTGGAG 
ATGCGCTACG GGGCTGATGT GGATGCCGGC TCCATTGTGC ACGCCGCCCA GAAGCTGGGC 
GAGCCTCCCG TGCTGCCCGT ATCTCGCATG GCCTCCATCC CCTCCATGAT CGGGGAGAAG 
CTGCCCACCG CCAAGGGGTT GGAGGCCGGG CTGGACACAC CCAAGGTAGC CACCAAAGGC 
AACTATATCG AGGTGCGCAC AGGCGCCGGC GGGGACGGTC TGGCTCGGCC CGAGGATGAC 
CTCCCGGACC TCGAGAACGG CCAGGGCTCG GCTGCAGAGA TCTCCACCAT TGCCAAGGAG 
GTGGACAAGG TCAACCAGAT CATTAACAAC TGCATCGATG CTCTCAAGCT GGACTCGGCC 
TCTTTTCTGG GAGGCGGCAG CAGCAGTGGG GACCCCGAGC TGGCCTTCGA GTGCCAGTCC 
CTCCCTGCAG CTGCTGCCGC CTCCTCAGCC ACTGGCCCCG GGGCCCTGGA GCGGCCCAGC 
TTCCTTTCGC CTCCCTACAA GGAGAGCTCC CACCACCCAC TACAGCGCCA GCTGAGCGCC 
GACGCGGCCG TGACCCGCAA GACCTGCAGC GTGTCGTCCA GTGGTTCCAT CAAGAGCGCC 
AAGGTCTTTA GCCTGGACGT GCCCGACCAT CCGGCCGCCA CAGGGCTGGC TAAGGGCGAC 
TCCAAGTACA TCGAGAAGGG CAGCCCCCTC AACAGCCCGC TGGACCGGCT CCCGCTGGTG 
CCGGCGGGCA GCGGCGGGGG CAGCGGCGGG GGCGGGGGCA TCCACCACCT GGAGGTGAAG 
CCGGCCTACC ACTGCAGCGA GCACCGGCAC AGCTTTCCCG CCCTGTACTA CGAGGAGGGT 
GCCGACAGCC TGAGCCAGCG CGTGTCCTTC CTCAAGCCGC TGACCCGCTC CAAGCGTGAC 
TCCACCTACT CGCAGCTCTC CCCCAGACAC TACTACTCAG GGTACTCCTC CAGCCCCGAG 
TACTCATCCG AGAGCACGCA CAAGATCTGG GAGCGCTTCC GGCCCTACAA GAAGCACCAC 
CGGGAGGAGG TGTACATGGC CGCCGGTCAC GCCCTGCGCA AGAAGGTCCA GTTCGCCAAG 
GACGAGGATC TGCATGACAT CCTTGATTAC TGGAAGGGGG TCTCCGCCCA GCAGAAGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
The pharmacogenomics journal14(4)356-64(2014 Aug)
Cobb J,Cule E,Moncrieffe H,Hinks A,Ursu S,Patrick F,Kassoumeri L,Flynn E,Bulatovi? M,Wulffraat N,van Zelst B,de Jonge R,Bohm M,Dolezalova P,Hirani S,Newman S,Whitworth P,Southwood TR,De Iorio M,,,,Wedderburn LR,Thomson W

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Chemistry & biology16(4)365-71(2009 Apr)
Hendrickx A,Beullens M,Ceulemans H,Den Abt T,Van Eynde A,Nicolaescu E,Lesage B,Bollen M

The extracellular leucine-rich repeat superfamily; a comparative survey and analysis of evolutionary relationships and expression patterns.
BMC genomics8320(2007 Sep)
Dolan J,Walshe K,Alsbury S,Hokamp K,O'Keeffe S,Okafuji T,Miller SF,Tear G,Mitchell KJ