NLRP3 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_004895.4	NP_004886.3	NACHT, LRR and PYD domains-containing protein 3 isoform a
XM_017000184.1	XP_016855673.1	NACHT, LRR and PYD domains-containing protein 3 isoform X4
XM_024452862.1	XP_024308630.1	NACHT, LRR and PYD domains-containing protein 3 isoform X3
NM_001243133.2	NP_001230062.1	NACHT, LRR and PYD domains-containing protein 3 isoform e
NM_001127462.3	NP_001120934.2	NACHT, LRR and PYD domains-containing protein 3 isoform d
NM_183395.3	NP_899632.2	NACHT, LRR and PYD domains-containing protein 3 isoform b
NM_001243133.1	NP_001230062.1	NACHT, LRR and PYD domains-containing protein 3 isoform e
XM_011544055.2	XP_011542357.1	NACHT, LRR and PYD domains-containing protein 3 isoform X5
NM_001079821.3	NP_001073289.2	NACHT, LRR and PYD domains-containing protein 3 isoform e
XM_011544053.2	XP_011542355.1	NACHT, LRR and PYD domains-containing protein 3 isoform X3
NM_001079821.2	NP_001073289.1	NACHT, LRR and PYD domains-containing protein 3 isoform a
NM_001127462.2	NP_001120934.1	NACHT, LRR and PYD domains-containing protein 3 isoform d
NM_001127461.2	NP_001120933.1	NACHT, LRR and PYD domains-containing protein 3 isoform c
XM_017000181.1	XP_016855670.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_017000182.1	XP_016855671.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_024452874.1	XP_024308642.1	NACHT, LRR and PYD domains-containing protein 3 isoform X5
NM_001127461.3	NP_001120933.2	NACHT, LRR and PYD domains-containing protein 3 isoform c
XM_017000183.1	XP_016855672.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2
NM_183395.2	NP_899632.1	NACHT, LRR and PYD domains-containing protein 3 isoform b
XM_011544048.2	XP_011542350.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
NM_004895.5	NP_004886.3	NACHT, LRR and PYD domains-containing protein 3 isoform a

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15874	NM_001243133.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu26851	NM_004895.4 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26851	NM_001079821.2 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26907	NM_183395.2 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$650.30 $929.00
OHu27058	NM_001127461.2 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
OHu27337	NM_001127462.2 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu34987	XM_017000183.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74741	XM_024452862.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74741	XM_011544053.2 Latest version!	Homo sapiens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74742	XM_017000184.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74743	XM_024452874.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu74743	XM_011544055.2 Latest version!	Homo sapiens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu109390	NM_183395.3 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu109444	NM_001127461.3 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu109486	NM_001127462.3 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu109389	NM_001079821.3 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu109389	NM_001243133.2 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu34986	NM_004895.5 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu34986	XM_017000182.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu34986	XM_011544048.2 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu34986	XM_017000181.1 Latest version!	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

NLRP3 ( NM_001243133.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230062.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu15874
Clone ID Related Accession (Same CDS sequence)	NM_001243133.1
Accession Version	NM_001243133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Hal M. Hoffman. The reference sequence was derived from AF427617.1, AC104335.2, AF410477.1, AL606804.11 and AF420469.2. Transcript Variant: This variant (6) is identical to variant 1 but uses a downstream start codon. The resulting protein (isoform e) is shorter at the N-terminus compared to isoform a. Sequence Note: This RefSeq initiates translation at a downstream start codon, resulting in a protein that is two aa shorter at the N-terminus compared to other isoforms. This RefSeq was created to support the clinical genetics community because the residue coordinates referred to by that community and the literature, including PMIDs:11687797, 11786556 and 12522564, are based on the use of the downstream start codon. No experimental evidence exists to show which start codon is preferentially used. The RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF410477.1, AF468522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11687797) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGG 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTCA GCTGCTGCCT CACATCAGCA 
TGTTGTCAGG ATCTTGCATC AGTATTGAGC ACCAGCCATT CCCTGACCAG ACTCTATGTG 
GGGGAGAATG CCTTGGGAGA CTCAGGAGTC GCAATTTTAT GTGAAAAAGC CAAGAATCCA 
CAGTGTAACC TGCAGAAACT GGGGTTGGTG AATTCTGGCC TTACGTCAGT CTGTTGTTCA 
GCTTTGTCCT CGGTACTCAG CACTAATCAG AATCTCACGC ACCTTTACCT GCGAGGCAAC 
ACTCTCGGAG ACAAGGGGAT CAAACTACTC TGTGAGGGAC TCTTGCACCC CGACTGCAAG 
CTTCAGGTGT TGGAATTAGA CAACTGCAAC CTCACGTCAC ACTGCTGCTG GGATCTTTCC 
ACACTTCTGA CCTCCAGCCA GAGCCTGCGA AAGCTGAGCC TGGGCAACAA TGACCTGGGC 
GACCTGGGGG TCATGATGTT CTGTGAAGTG CTGAAACAGC AGAGCTGCCT CCTGCAGAAC 
CTGGGGTTGT CTGAAATGTA TTTCAATTAT GAGACAAAAA GTGCGTTAGA AACACTTCAA 
GAAGAAAAGC CTGAGCTGAC CGTCGTCTTT GAGCCTTCTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230062.1
CDS	753..3857
Translation	MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001243133.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243133.1

ORF Insert Sequence:

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGG 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTCA GCTGCTGCCT CACATCAGCA 
TGTTGTCAGG ATCTTGCATC AGTATTGAGC ACCAGCCATT CCCTGACCAG ACTCTATGTG 
GGGGAGAATG CCTTGGGAGA CTCAGGAGTC GCAATTTTAT GTGAAAAAGC CAAGAATCCA 
CAGTGTAACC TGCAGAAACT GGGGTTGGTG AATTCTGGCC TTACGTCAGT CTGTTGTTCA 
GCTTTGTCCT CGGTACTCAG CACTAATCAG AATCTCACGC ACCTTTACCT GCGAGGCAAC 
ACTCTCGGAG ACAAGGGGAT CAAACTACTC TGTGAGGGAC TCTTGCACCC CGACTGCAAG 
CTTCAGGTGT TGGAATTAGA CAACTGCAAC CTCACGTCAC ACTGCTGCTG GGATCTTTCC 
ACACTTCTGA CCTCCAGCCA GAGCCTGCGA AAGCTGAGCC TGGGCAACAA TGACCTGGGC 
GACCTGGGGG TCATGATGTT CTGTGAAGTG CTGAAACAGC AGAGCTGCCT CCTGCAGAAC 
CTGGGGTTGT CTGAAATGTA TTTCAATTAT GAGACAAAAA GTGCGTTAGA AACACTTCAA 
GAAGAAAAGC CTGAGCTGAC CGTCGTCTTT GAGCCTTCTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_004895.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004886.3 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26851
Clone ID Related Accession (Same CDS sequence)	NM_004895.4 , NM_001079821.2
Accession Version	NM_004895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF427617.1, AC104335.2, AF410477.1, AL606804.11 and AF420469.2. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2008 this sequence version replaced NM_004895.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF410477.1, AF468522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004886.3
CDS	747..3857
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_004895.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004895.4

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001079821.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073289.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 3, mRNA.

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CloneID	OHu26851
Clone ID Related Accession (Same CDS sequence)	NM_004895.4 , NM_001079821.2
Accession Version	NM_001079821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314998.1, AF410477.1, AL606804.11 and AF420469.2. On Oct 7, 2008 this sequence version replaced NM_001079821.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF420469.2, AK314998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073289.1
CDS	139..3249
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001079821.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079821.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_183395.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_899632.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26907
Clone ID Related Accession (Same CDS sequence)	NM_183395.2
Accession Version	NM_183395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF427617.1, AC104335.2, AF410477.1, AY092033.1, AL606804.11 and AF420469.2. On Oct 7, 2008 this sequence version replaced NM_183395.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons, which are located in the mid-coding and 3' coding regions, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF427617.1, AY092033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899632.1
CDS	747..3515
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_183395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183395.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001127461.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120933.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27058
Clone ID Related Accession (Same CDS sequence)	NM_001127461.2
Accession Version	NM_001127461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314998.1, AB208891.1, AC104335.2, AL606804.11 and AF420469.2. On Oct 7, 2008 this sequence version replaced NM_001127461.1. Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTGAATTC TGGCCTTACG 
TCAGTCTGTT GTTCAGCTTT GTCCTCGGTA CTCAGCACTA ATCAGAATCT CACGCACCTT 
TACCTGCGAG GCAACACTCT CGGAGACAAG GGGATCAAAC TACTCTGTGA GGGACTCTTG 
CACCCCGACT GCAAGCTTCA GGTGTTGGAA TTAGACAACT GCAACCTCAC GTCACACTGC 
TGCTGGGATC TTTCCACACT TCTGACCTCC AGCCAGAGCC TGCGAAAGCT GAGCCTGGGC 
AACAATGACC TGGGCGACCT GGGGGTCATG ATGTTCTGTG AAGTGCTGAA ACAGCAGAGC 
TGCCTCCTGC AGAACCTGGG GTTGTCTGAA ATGTATTTCA ATTATGAGAC AAAAAGTGCG 
TTAGAAACAC TTCAAGAAGA AAAGCCTGAG CTGACCGTCG TCTTTGAGCC TTCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120933.1
CDS	781..3720
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001127461.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127461.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTGAATTC TGGCCTTACG 
TCAGTCTGTT GTTCAGCTTT GTCCTCGGTA CTCAGCACTA ATCAGAATCT CACGCACCTT 
TACCTGCGAG GCAACACTCT CGGAGACAAG GGGATCAAAC TACTCTGTGA GGGACTCTTG 
CACCCCGACT GCAAGCTTCA GGTGTTGGAA TTAGACAACT GCAACCTCAC GTCACACTGC 
TGCTGGGATC TTTCCACACT TCTGACCTCC AGCCAGAGCC TGCGAAAGCT GAGCCTGGGC 
AACAATGACC TGGGCGACCT GGGGGTCATG ATGTTCTGTG AAGTGCTGAA ACAGCAGAGC 
TGCCTCCTGC AGAACCTGGG GTTGTCTGAA ATGTATTTCA ATTATGAGAC AAAAAGTGCG 
TTAGAAACAC TTCAAGAAGA AAAGCCTGAG CTGACCGTCG TCTTTGAGCC TTCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001127462.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120934.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 5, mRNA.

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CloneID	OHu27337
Clone ID Related Accession (Same CDS sequence)	NM_001127462.2
Accession Version	NM_001127462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF410477.1, AC104335.2, AY422168.1, AL606804.11 and AF420469.2. On Oct 7, 2008 this sequence version replaced NM_001127462.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the mid-coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117211.1, AY422168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTCAGCTGC 
TGCCTCACAT CAGCATGTTG TCAGGATCTT GCATCAGTAT TGAGCACCAG CCATTCCCTG 
ACCAGACTCT ATGTGGGGGA GAATGCCTTG GGAGACTCAG GAGTCGCAAT TTTATGTGAA 
AAAGCCAAGA ATCCACAGTG TAACCTGCAG AAACTGGGGT TGGTGAATTC TGGCCTTACG 
TCAGTCTGTT GTTCAGCTTT GTCCTCGGTA CTCAGCACTA ATCAGAATCT CACGCACCTT 
TACCTGCGAG GCAACACTCT CGGAGACAAG GGGATCAAAC TACTCTGTGA GGGACTCTTG 
CACCCCGACT GCAAGCTTCA GGTGTTGGAA TTAGACAACT GCAACCTCAC GTCACACTGC 
TGCTGGGATC TTTCCACACT TCTGACCTCC AGCCAGAGCC TGCGAAAGCT GAGCCTGGGC 
AACAATGACC TGGGCGACCT GGGGGTCATG ATGTTCTGTG AAGTGCTGAA ACAGCAGAGC 
TGCCTCCTGC AGAACCTGGG GTTGTCTGAA ATGTATTTCA ATTATGAGAC AAAAAGTGCG 
TTAGAAACAC TTCAAGAAGA AAAGCCTGAG CTGACCGTCG TCTTTGAGCC TTCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120934.1
CDS	747..3686
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001127462.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127462.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTCAGCTGC 
TGCCTCACAT CAGCATGTTG TCAGGATCTT GCATCAGTAT TGAGCACCAG CCATTCCCTG 
ACCAGACTCT ATGTGGGGGA GAATGCCTTG GGAGACTCAG GAGTCGCAAT TTTATGTGAA 
AAAGCCAAGA ATCCACAGTG TAACCTGCAG AAACTGGGGT TGGTGAATTC TGGCCTTACG 
TCAGTCTGTT GTTCAGCTTT GTCCTCGGTA CTCAGCACTA ATCAGAATCT CACGCACCTT 
TACCTGCGAG GCAACACTCT CGGAGACAAG GGGATCAAAC TACTCTGTGA GGGACTCTTG 
CACCCCGACT GCAAGCTTCA GGTGTTGGAA TTAGACAACT GCAACCTCAC GTCACACTGC 
TGCTGGGATC TTTCCACACT TCTGACCTCC AGCCAGAGCC TGCGAAAGCT GAGCCTGGGC 
AACAATGACC TGGGCGACCT GGGGGTCATG ATGTTCTGTG AAGTGCTGAA ACAGCAGAGC 
TGCCTCCTGC AGAACCTGGG GTTGTCTGAA ATGTATTTCA ATTATGAGAC AAAAAGTGCG 
TTAGAAACAC TTCAAGAAGA AAAGCCTGAG CTGACCGTCG TCTTTGAGCC TTCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_017000183.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855672.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu34987
Clone ID Related Accession (Same CDS sequence)	XM_017000183.1
Accession Version	XM_017000183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTCAGCTGC 
TGCCTCACAT CAGCATGTTG TCAGGATCTT GCATCAGTAT TGAGCACCAG CCATTCCCTG 
ACCAGACTCT ATGTGGGGGA GAATGCCTTG GGAGACTCAG GAGTCGCAAT TTTATGTGAA 
AAAGCCAAGA ATCCACAGTG TAACCTGCAG AAACTGGGGT TGGTGAATTC TGGCCTTACG 
TCAGTCTGTT GTTCAGCTTT GTCCTCGGTA CTCAGCACTA ATCAGAATCT CACGCACCTT 
TACCTGCGAG GCAACACTCT CGGAGACAAG GGGATCAAAC TACTCTGTGA GGGACTCTTG 
CACCCCGACT GCAAGCTTCA GGTGTTGGAA TTAGACAACT GCAACCTCAC GTCACACTGC 
TGCTGGGATC TTTCCACACT TCTGACCTCC AGCCAGAGCC TGCGAAAGCT GAGCCTGGGC 
AACAATGACC TGGGCGACCT GGGGGTCATG ATGTTCTGTG AAGTGCTGAA ACAGCAGAGC 
TGCCTCCTGC AGAACCTGGG GTTGTCTGAA ATGTATTTCA ATTATGAGAC AAAAAGTGCG 
TTAGAAACAC TTCAAGAAGA AAAGCCTGAG CTGACCGTCG TCTTTGAGCC TTCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855672.1
CDS	774..3713
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_017000183.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000183.1

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTCAGCTGC 
TGCCTCACAT CAGCATGTTG TCAGGATCTT GCATCAGTAT TGAGCACCAG CCATTCCCTG 
ACCAGACTCT ATGTGGGGGA GAATGCCTTG GGAGACTCAG GAGTCGCAAT TTTATGTGAA 
AAAGCCAAGA ATCCACAGTG TAACCTGCAG AAACTGGGGT TGGTGAATTC TGGCCTTACG 
TCAGTCTGTT GTTCAGCTTT GTCCTCGGTA CTCAGCACTA ATCAGAATCT CACGCACCTT 
TACCTGCGAG GCAACACTCT CGGAGACAAG GGGATCAAAC TACTCTGTGA GGGACTCTTG 
CACCCCGACT GCAAGCTTCA GGTGTTGGAA TTAGACAACT GCAACCTCAC GTCACACTGC 
TGCTGGGATC TTTCCACACT TCTGACCTCC AGCCAGAGCC TGCGAAAGCT GAGCCTGGGC 
AACAATGACC TGGGCGACCT GGGGGTCATG ATGTTCTGTG AAGTGCTGAA ACAGCAGAGC 
TGCCTCCTGC AGAACCTGGG GTTGTCTGAA ATGTATTTCA ATTATGAGAC AAAAAGTGCG 
TTAGAAACAC TTCAAGAAGA AAAGCCTGAG CTGACCGTCG TCTTTGAGCC TTCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_024452862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308630.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu74741
Clone ID Related Accession (Same CDS sequence)	XM_024452862.1 , XM_011544053.2
Accession Version	XM_024452862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308630.1
CDS	886..3654
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_024452862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452862.1

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_011544053.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542355.1 Homo sapiens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu74741
Clone ID Related Accession (Same CDS sequence)	XM_024452862.1 , XM_011544053.2
Accession Version	XM_011544053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542355.1
CDS	774..3542
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_011544053.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544053.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_017000184.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855673.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu74742
Clone ID Related Accession (Same CDS sequence)	XM_017000184.1
Accession Version	XM_017000184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855673.1
CDS	774..3542
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_017000184.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000184.1

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GGGCGCTGTG GCCTCTCGCA TGAGTGCTGC TTCGACATCT CCTTGGTCCT CAGCAGCAAC 
CAGAAGCTGG TGGAGCTGGA CCTGAGTGAC AACGCCCTCG GTGACTTCGG AATCAGACTT 
CTGTGTGTGG GACTGAAGCA CCTGTTGTGC AATCTGAAGA AGCTCTGGTT GGTGAATTCT 
GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA TCAGAATCTC 
ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT ACTCTGTGAG 
GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG CAACCTCACG 
TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT GCGAAAGCTG 
AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA AGTGCTGAAA 
CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA TTATGAGACA 
AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT CTTTGAGCCT 
TCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_011544055.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542357.1 Homo sapiens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu74743
Clone ID Related Accession (Same CDS sequence)	XM_011544055.2 , XM_024452874.1
Accession Version	XM_011544055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GTGAATTCTG GCCTTACGTC AGTCTGTTGT TCAGCTTTGT CCTCGGTACT CAGCACTAAT 
CAGAATCTCA CGCACCTTTA CCTGCGAGGC AACACTCTCG GAGACAAGGG GATCAAACTA 
CTCTGTGAGG GACTCTTGCA CCCCGACTGC AAGCTTCAGG TGTTGGAATT AGACAACTGC 
AACCTCACGT CACACTGCTG CTGGGATCTT TCCACACTTC TGACCTCCAG CCAGAGCCTG 
CGAAAGCTGA GCCTGGGCAA CAATGACCTG GGCGACCTGG GGGTCATGAT GTTCTGTGAA 
GTGCTGAAAC AGCAGAGCTG CCTCCTGCAG AACCTGGGGT TGTCTGAAAT GTATTTCAAT 
TATGAGACAA AAAGTGCGTT AGAAACACTT CAAGAAGAAA AGCCTGAGCT GACCGTCGTC 
TTTGAGCCTT CTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542357.1
CDS	774..3371
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_011544055.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544055.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GTGAATTCTG GCCTTACGTC AGTCTGTTGT TCAGCTTTGT CCTCGGTACT CAGCACTAAT 
CAGAATCTCA CGCACCTTTA CCTGCGAGGC AACACTCTCG GAGACAAGGG GATCAAACTA 
CTCTGTGAGG GACTCTTGCA CCCCGACTGC AAGCTTCAGG TGTTGGAATT AGACAACTGC 
AACCTCACGT CACACTGCTG CTGGGATCTT TCCACACTTC TGACCTCCAG CCAGAGCCTG 
CGAAAGCTGA GCCTGGGCAA CAATGACCTG GGCGACCTGG GGGTCATGAT GTTCTGTGAA 
GTGCTGAAAC AGCAGAGCTG CCTCCTGCAG AACCTGGGGT TGTCTGAAAT GTATTTCAAT 
TATGAGACAA AAAGTGCGTT AGAAACACTT CAAGAAGAAA AGCCTGAGCT GACCGTCGTC 
TTTGAGCCTT CTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_024452874.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308642.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X7, mRNA.

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CloneID	OHu74743
Clone ID Related Accession (Same CDS sequence)	XM_011544055.2 , XM_024452874.1
Accession Version	XM_024452874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GTGAATTCTG GCCTTACGTC AGTCTGTTGT TCAGCTTTGT CCTCGGTACT CAGCACTAAT 
CAGAATCTCA CGCACCTTTA CCTGCGAGGC AACACTCTCG GAGACAAGGG GATCAAACTA 
CTCTGTGAGG GACTCTTGCA CCCCGACTGC AAGCTTCAGG TGTTGGAATT AGACAACTGC 
AACCTCACGT CACACTGCTG CTGGGATCTT TCCACACTTC TGACCTCCAG CCAGAGCCTG 
CGAAAGCTGA GCCTGGGCAA CAATGACCTG GGCGACCTGG GGGTCATGAT GTTCTGTGAA 
GTGCTGAAAC AGCAGAGCTG CCTCCTGCAG AACCTGGGGT TGTCTGAAAT GTATTTCAAT 
TATGAGACAA AAAGTGCGTT AGAAACACTT CAAGAAGAAA AGCCTGAGCT GACCGTCGTC 
TTTGAGCCTT CTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308642.1
CDS	886..3483
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_024452874.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452874.1

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGGTTG 
GTGAATTCTG GCCTTACGTC AGTCTGTTGT TCAGCTTTGT CCTCGGTACT CAGCACTAAT 
CAGAATCTCA CGCACCTTTA CCTGCGAGGC AACACTCTCG GAGACAAGGG GATCAAACTA 
CTCTGTGAGG GACTCTTGCA CCCCGACTGC AAGCTTCAGG TGTTGGAATT AGACAACTGC 
AACCTCACGT CACACTGCTG CTGGGATCTT TCCACACTTC TGACCTCCAG CCAGAGCCTG 
CGAAAGCTGA GCCTGGGCAA CAATGACCTG GGCGACCTGG GGGTCATGAT GTTCTGTGAA 
GTGCTGAAAC AGCAGAGCTG CCTCCTGCAG AACCTGGGGT TGTCTGAAAT GTATTTCAAT 
TATGAGACAA AAAGTGCGTT AGAAACACTT CAAGAAGAAA AGCCTGAGCT GACCGTCGTC 
TTTGAGCCTT CTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_183395.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_899632.2 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 2, mRNA.

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CloneID	OHu109390
Clone ID Related Accession (Same CDS sequence)	NM_183395.3
Accession Version	NM_183395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104335.2, AF427617.1, AF410477.1, AY092033.1 and AL606804.11. On or before Dec 17, 2019 this sequence version replaced XM_017000184.1, NM_183395.2. Transcript Variant: This variant (2) lacks two alternate in-frame exons, which are located in the mid-coding and 3' coding regions, compared to variant 6. The encoded isoform (b) is shorter than isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY092033.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11687797) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG GTTGGGGCGC 
TGTGGCCTCT CGCATGAGTG CTGCTTCGAC ATCTCCTTGG TCCTCAGCAG CAACCAGAAG 
CTGGTGGAGC TGGACCTGAG TGACAACGCC CTCGGTGACT TCGGAATCAG ACTTCTGTGT 
GTGGGACTGA AGCACCTGTT GTGCAATCTG AAGAAGCTCT GGTTGGTGAA TTCTGGCCTT 
ACGTCAGTCT GTTGTTCAGC TTTGTCCTCG GTACTCAGCA CTAATCAGAA TCTCACGCAC 
CTTTACCTGC GAGGCAACAC TCTCGGAGAC AAGGGGATCA AACTACTCTG TGAGGGACTC 
TTGCACCCCG ACTGCAAGCT TCAGGTGTTG GAATTAGACA ACTGCAACCT CACGTCACAC 
TGCTGCTGGG ATCTTTCCAC ACTTCTGACC TCCAGCCAGA GCCTGCGAAA GCTGAGCCTG 
GGCAACAATG ACCTGGGCGA CCTGGGGGTC ATGATGTTCT GTGAAGTGCT GAAACAGCAG 
AGCTGCCTCC TGCAGAACCT GGGGTTGTCT GAAATGTATT TCAATTATGA GACAAAAAGT 
GCGTTAGAAA CACTTCAAGA AGAAAAGCCT GAGCTGACCG TCGTCTTTGA GCCTTCTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899632.2
CDS	770..3532
Translation	MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_183395.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183395.3

ORF Insert Sequence:

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG GTTGGGGCGC 
TGTGGCCTCT CGCATGAGTG CTGCTTCGAC ATCTCCTTGG TCCTCAGCAG CAACCAGAAG 
CTGGTGGAGC TGGACCTGAG TGACAACGCC CTCGGTGACT TCGGAATCAG ACTTCTGTGT 
GTGGGACTGA AGCACCTGTT GTGCAATCTG AAGAAGCTCT GGTTGGTGAA TTCTGGCCTT 
ACGTCAGTCT GTTGTTCAGC TTTGTCCTCG GTACTCAGCA CTAATCAGAA TCTCACGCAC 
CTTTACCTGC GAGGCAACAC TCTCGGAGAC AAGGGGATCA AACTACTCTG TGAGGGACTC 
TTGCACCCCG ACTGCAAGCT TCAGGTGTTG GAATTAGACA ACTGCAACCT CACGTCACAC 
TGCTGCTGGG ATCTTTCCAC ACTTCTGACC TCCAGCCAGA GCCTGCGAAA GCTGAGCCTG 
GGCAACAATG ACCTGGGCGA CCTGGGGGTC ATGATGTTCT GTGAAGTGCT GAAACAGCAG 
AGCTGCCTCC TGCAGAACCT GGGGTTGTCT GAAATGTATT TCAATTATGA GACAAAAAGT 
GCGTTAGAAA CACTTCAAGA AGAAAAGCCT GAGCTGACCG TCGTCTTTGA GCCTTCTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001127461.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120933.2 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu109444
Clone ID Related Accession (Same CDS sequence)	NM_001127461.3
Accession Version	NM_001127461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314998.1, AB208891.1 and AC104335.2. On Dec 17, 2019 this sequence version replaced NM_001127461.2. Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 6. The encoded isoform (c) is shorter than isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208891.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11687797) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTGA ATTCTGGCCT TACGTCAGTC 
TGTTGTTCAG CTTTGTCCTC GGTACTCAGC ACTAATCAGA ATCTCACGCA CCTTTACCTG 
CGAGGCAACA CTCTCGGAGA CAAGGGGATC AAACTACTCT GTGAGGGACT CTTGCACCCC 
GACTGCAAGC TTCAGGTGTT GGAATTAGAC AACTGCAACC TCACGTCACA CTGCTGCTGG 
GATCTTTCCA CACTTCTGAC CTCCAGCCAG AGCCTGCGAA AGCTGAGCCT GGGCAACAAT 
GACCTGGGCG ACCTGGGGGT CATGATGTTC TGTGAAGTGC TGAAACAGCA GAGCTGCCTC 
CTGCAGAACC TGGGGTTGTC TGAAATGTAT TTCAATTATG AGACAAAAAG TGCGTTAGAA 
ACACTTCAAG AAGAAAAGCC TGAGCTGACC GTCGTCTTTG AGCCTTCTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120933.2
CDS	770..3703
Translation	MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001127461.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127461.3

ORF Insert Sequence:

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTGA ATTCTGGCCT TACGTCAGTC 
TGTTGTTCAG CTTTGTCCTC GGTACTCAGC ACTAATCAGA ATCTCACGCA CCTTTACCTG 
CGAGGCAACA CTCTCGGAGA CAAGGGGATC AAACTACTCT GTGAGGGACT CTTGCACCCC 
GACTGCAAGC TTCAGGTGTT GGAATTAGAC AACTGCAACC TCACGTCACA CTGCTGCTGG 
GATCTTTCCA CACTTCTGAC CTCCAGCCAG AGCCTGCGAA AGCTGAGCCT GGGCAACAAT 
GACCTGGGCG ACCTGGGGGT CATGATGTTC TGTGAAGTGC TGAAACAGCA GAGCTGCCTC 
CTGCAGAACC TGGGGTTGTC TGAAATGTAT TTCAATTATG AGACAAAAAG TGCGTTAGAA 
ACACTTCAAG AAGAAAAGCC TGAGCTGACC GTCGTCTTTG AGCCTTCTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001127462.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120934.2 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu109486
Clone ID Related Accession (Same CDS sequence)	NM_001127462.3
Accession Version	NM_001127462.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104335.2, AF410477.1, AY422168.1 and AL606804.11. On or before Dec 17, 2019 this sequence version replaced XM_017000183.1, NM_001127462.2. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the mid-coding region, compared to variant 6. The encoded isoform (d) is shorter than isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC117211.1, AY422168.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11687797) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG GTTGGGGCGC 
TGTGGCCTCT CGCATGAGTG CTGCTTCGAC ATCTCCTTGG TCCTCAGCAG CAACCAGAAG 
CTGGTGGAGC TGGACCTGAG TGACAACGCC CTCGGTGACT TCGGAATCAG ACTTCTGTGT 
GTGGGACTGA AGCACCTGTT GTGCAATCTG AAGAAGCTCT GGTTGGTCAG CTGCTGCCTC 
ACATCAGCAT GTTGTCAGGA TCTTGCATCA GTATTGAGCA CCAGCCATTC CCTGACCAGA 
CTCTATGTGG GGGAGAATGC CTTGGGAGAC TCAGGAGTCG CAATTTTATG TGAAAAAGCC 
AAGAATCCAC AGTGTAACCT GCAGAAACTG GGGTTGGTGA ATTCTGGCCT TACGTCAGTC 
TGTTGTTCAG CTTTGTCCTC GGTACTCAGC ACTAATCAGA ATCTCACGCA CCTTTACCTG 
CGAGGCAACA CTCTCGGAGA CAAGGGGATC AAACTACTCT GTGAGGGACT CTTGCACCCC 
GACTGCAAGC TTCAGGTGTT GGAATTAGAC AACTGCAACC TCACGTCACA CTGCTGCTGG 
GATCTTTCCA CACTTCTGAC CTCCAGCCAG AGCCTGCGAA AGCTGAGCCT GGGCAACAAT 
GACCTGGGCG ACCTGGGGGT CATGATGTTC TGTGAAGTGC TGAAACAGCA GAGCTGCCTC 
CTGCAGAACC TGGGGTTGTC TGAAATGTAT TTCAATTATG AGACAAAAAG TGCGTTAGAA 
ACACTTCAAG AAGAAAAGCC TGAGCTGACC GTCGTCTTTG AGCCTTCTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120934.2
CDS	770..3703
Translation	MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001127462.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127462.3

ORF Insert Sequence:

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG GTTGGGGCGC 
TGTGGCCTCT CGCATGAGTG CTGCTTCGAC ATCTCCTTGG TCCTCAGCAG CAACCAGAAG 
CTGGTGGAGC TGGACCTGAG TGACAACGCC CTCGGTGACT TCGGAATCAG ACTTCTGTGT 
GTGGGACTGA AGCACCTGTT GTGCAATCTG AAGAAGCTCT GGTTGGTCAG CTGCTGCCTC 
ACATCAGCAT GTTGTCAGGA TCTTGCATCA GTATTGAGCA CCAGCCATTC CCTGACCAGA 
CTCTATGTGG GGGAGAATGC CTTGGGAGAC TCAGGAGTCG CAATTTTATG TGAAAAAGCC 
AAGAATCCAC AGTGTAACCT GCAGAAACTG GGGTTGGTGA ATTCTGGCCT TACGTCAGTC 
TGTTGTTCAG CTTTGTCCTC GGTACTCAGC ACTAATCAGA ATCTCACGCA CCTTTACCTG 
CGAGGCAACA CTCTCGGAGA CAAGGGGATC AAACTACTCT GTGAGGGACT CTTGCACCCC 
GACTGCAAGC TTCAGGTGTT GGAATTAGAC AACTGCAACC TCACGTCACA CTGCTGCTGG 
GATCTTTCCA CACTTCTGAC CTCCAGCCAG AGCCTGCGAA AGCTGAGCCT GGGCAACAAT 
GACCTGGGCG ACCTGGGGGT CATGATGTTC TGTGAAGTGC TGAAACAGCA GAGCTGCCTC 
CTGCAGAACC TGGGGTTGTC TGAAATGTAT TTCAATTATG AGACAAAAAG TGCGTTAGAA 
ACACTTCAAG AAGAAAAGCC TGAGCTGACC GTCGTCTTTG AGCCTTCTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001243133.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230062.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 6, mRNA.

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CloneID	OHu109389
Clone ID Related Accession (Same CDS sequence)	NM_001243133.2 , NM_001079821.3
Accession Version	NM_001243133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Hal M. Hoffman. The reference sequence was derived from AC104335.2, AF427617.1 and AF410477.1. On Dec 17, 2019 this sequence version replaced NM_001243133.1. Transcript Variant: This variant (6) represents the longest transcript and encodes isoform e. Variants 1 and 6 represent the same transcript but use different translation start sites since both sites are likely used. The encoded isoform (e) uses the most conserved translation start site. Both variants 3 and 6 encode the same isoform (e). Sequence Note: The RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF468522.1, BC143362.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11687797) RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTCA GCTGCTGCCT CACATCAGCA 
TGTTGTCAGG ATCTTGCATC AGTATTGAGC ACCAGCCATT CCCTGACCAG ACTCTATGTG 
GGGGAGAATG CCTTGGGAGA CTCAGGAGTC GCAATTTTAT GTGAAAAAGC CAAGAATCCA 
CAGTGTAACC TGCAGAAACT GGGGTTGGTG AATTCTGGCC TTACGTCAGT CTGTTGTTCA 
GCTTTGTCCT CGGTACTCAG CACTAATCAG AATCTCACGC ACCTTTACCT GCGAGGCAAC 
ACTCTCGGAG ACAAGGGGAT CAAACTACTC TGTGAGGGAC TCTTGCACCC CGACTGCAAG 
CTTCAGGTGT TGGAATTAGA CAACTGCAAC CTCACGTCAC ACTGCTGCTG GGATCTTTCC 
ACACTTCTGA CCTCCAGCCA GAGCCTGCGA AAGCTGAGCC TGGGCAACAA TGACCTGGGC 
GACCTGGGGG TCATGATGTT CTGTGAAGTG CTGAAACAGC AGAGCTGCCT CCTGCAGAAC 
CTGGGGTTGT CTGAAATGTA TTTCAATTAT GAGACAAAAA GTGCGTTAGA AACACTTCAA 
GAAGAAAAGC CTGAGCTGAC CGTCGTCTTT GAGCCTTCTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230062.1
CDS	770..3874
Translation	MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001243133.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243133.2

ORF Insert Sequence:

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTCA GCTGCTGCCT CACATCAGCA 
TGTTGTCAGG ATCTTGCATC AGTATTGAGC ACCAGCCATT CCCTGACCAG ACTCTATGTG 
GGGGAGAATG CCTTGGGAGA CTCAGGAGTC GCAATTTTAT GTGAAAAAGC CAAGAATCCA 
CAGTGTAACC TGCAGAAACT GGGGTTGGTG AATTCTGGCC TTACGTCAGT CTGTTGTTCA 
GCTTTGTCCT CGGTACTCAG CACTAATCAG AATCTCACGC ACCTTTACCT GCGAGGCAAC 
ACTCTCGGAG ACAAGGGGAT CAAACTACTC TGTGAGGGAC TCTTGCACCC CGACTGCAAG 
CTTCAGGTGT TGGAATTAGA CAACTGCAAC CTCACGTCAC ACTGCTGCTG GGATCTTTCC 
ACACTTCTGA CCTCCAGCCA GAGCCTGCGA AAGCTGAGCC TGGGCAACAA TGACCTGGGC 
GACCTGGGGG TCATGATGTT CTGTGAAGTG CTGAAACAGC AGAGCTGCCT CCTGCAGAAC 
CTGGGGTTGT CTGAAATGTA TTTCAATTAT GAGACAAAAA GTGCGTTAGA AACACTTCAA 
GAAGAAAAGC CTGAGCTGAC CGTCGTCTTT GAGCCTTCTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001079821.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073289.2 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu109389
Clone ID Related Accession (Same CDS sequence)	NM_001243133.2 , NM_001079821.3
Accession Version	NM_001079821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314998.1, AF410477.1, AC104335.2 and AL606804.11. On Dec 19, 2019 this sequence version replaced NM_001079821.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 6. Both variants 3 and 6 encode the same isoform (e). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF420469.2, AK314998.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11687797) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTCA GCTGCTGCCT CACATCAGCA 
TGTTGTCAGG ATCTTGCATC AGTATTGAGC ACCAGCCATT CCCTGACCAG ACTCTATGTG 
GGGGAGAATG CCTTGGGAGA CTCAGGAGTC GCAATTTTAT GTGAAAAAGC CAAGAATCCA 
CAGTGTAACC TGCAGAAACT GGGGTTGGTG AATTCTGGCC TTACGTCAGT CTGTTGTTCA 
GCTTTGTCCT CGGTACTCAG CACTAATCAG AATCTCACGC ACCTTTACCT GCGAGGCAAC 
ACTCTCGGAG ACAAGGGGAT CAAACTACTC TGTGAGGGAC TCTTGCACCC CGACTGCAAG 
CTTCAGGTGT TGGAATTAGA CAACTGCAAC CTCACGTCAC ACTGCTGCTG GGATCTTTCC 
ACACTTCTGA CCTCCAGCCA GAGCCTGCGA AAGCTGAGCC TGGGCAACAA TGACCTGGGC 
GACCTGGGGG TCATGATGTT CTGTGAAGTG CTGAAACAGC AGAGCTGCCT CCTGCAGAAC 
CTGGGGTTGT CTGAAATGTA TTTCAATTAT GAGACAAAAA GTGCGTTAGA AACACTTCAA 
GAAGAAAAGC CTGAGCTGAC CGTCGTCTTT GAGCCTTCTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073289.2
CDS	128..3232
Translation	MASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_001079821.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079821.3

ORF Insert Sequence:

ATGGCAAGCA CCCGCTGCAA GCTGGCCAGG TACCTGGAGG ACCTGGAGGA TGTGGACTTG 
AAGAAATTTA AGATGCACTT AGAGGACTAT CCTCCCCAGA AGGGCTGCAT CCCCCTCCCG 
AGGGGTCAGA CAGAGAAGGC AGACCATGTG GATCTAGCCA CGCTAATGAT CGACTTCAAT 
GGGGAGGAGA AGGCGTGGGC CATGGCCGTG TGGATCTTCG CTGCGATCAA CAGGAGAGAC 
CTTTATGAGA AAGCAAAAAG AGATGAGCCG AAGTGGGGTT CAGATAATGC ACGTGTTTCG 
AATCCCACTG TGATATGCCA GGAAGACAGC ATTGAAGAGG AGTGGATGGG TTTACTGGAG 
TACCTTTCGA GAATCTCTAT TTGTAAAATG AAGAAAGATT ACCGTAAGAA GTACAGAAAG 
TACGTGAGAA GCAGATTCCA GTGCATTGAA GACAGGAATG CCCGTCTGGG TGAGAGTGTG 
AGCCTCAACA AACGCTACAC ACGACTGCGT CTCATCAAGG AGCACCGGAG CCAGCAGGAG 
AGGGAGCAGG AGCTTCTGGC CATCGGCAAG ACCAAGACGT GTGAGAGCCC CGTGAGTCCC 
ATTAAGATGG AGTTGCTGTT TGACCCCGAT GATGAGCATT CTGAGCCTGT GCACACCGTG 
GTGTTCCAGG GGGCGGCAGG GATTGGGAAA ACAATCCTGG CCAGGAAGAT GATGTTGGAC 
TGGGCGTCGG GGACACTCTA CCAAGACAGG TTTGACTATC TGTTCTATAT CCACTGTCGA 
GAGGTGAGCC TTGTGACACA GAGGAGCCTG GGGGACCTGA TCATGAGCTG CTGCCCCGAC 
CCAAACCCAC CCATCCACAA GATCGTGAGA AAACCCTCCA GAATCCTCTT CCTCATGGAC 
GGCTTCGATG AGCTGCAAGG TGCCTTTGAC GAGCACATAG GACCGCTCTG CACTGACTGG 
CAGAAGGCCG AGCGGGGAGA CATTCTCCTG AGCAGCCTCA TCAGAAAGAA GCTGCTTCCC 
GAGGCCTCTC TGCTCATCAC CACGAGACCT GTGGCCCTGG AGAAACTGCA GCACTTGCTG 
GACCATCCTC GGCATGTGGA GATCCTGGGT TTCTCCGAGG CCAAAAGGAA AGAGTACTTC 
TTCAAGTACT TCTCTGATGA GGCCCAAGCC AGGGCAGCCT TCAGTCTGAT TCAGGAGAAC 
GAGGTCCTCT TCACCATGTG CTTCATCCCC CTGGTCTGCT GGATCGTGTG CACTGGACTG 
AAACAGCAGA TGGAGAGTGG CAAGAGCCTT GCCCAGACAT CCAAGACCAC CACCGCGGTG 
TACGTCTTCT TCCTTTCCAG TTTGCTGCAG CCCCGGGGAG GGAGCCAGGA GCACGGCCTC 
TGCGCCCACC TCTGGGGGCT CTGCTCTTTG GCTGCAGATG GAATCTGGAA CCAGAAAATC 
CTGTTTGAGG AGTCCGACCT CAGGAATCAT GGACTGCAGA AGGCGGATGT GTCTGCTTTC 
CTGAGGATGA ACCTGTTCCA AAAGGAAGTG GACTGCGAGA AGTTCTACAG CTTCATCCAC 
ATGACTTTCC AGGAGTTCTT TGCCGCCATG TACTACCTGC TGGAAGAGGA AAAGGAAGGA 
AGGACGAACG TTCCAGGGAG TCGTTTGAAG CTTCCCAGCC GAGACGTGAC AGTCCTTCTG 
GAAAACTATG GCAAATTCGA AAAGGGGTAT TTGATTTTTG TTGTACGTTT CCTCTTTGGC 
CTGGTAAACC AGGAGAGGAC CTCCTACTTG GAGAAGAAAT TAAGTTGCAA GATCTCTCAG 
CAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGTGAAAG CCAAAGCTAA AAAGCTGCAG 
ATCCAGCCCA GCCAGCTGGA ATTGTTCTAC TGTTTGTACG AGATGCAGGA GGAGGACTTC 
GTGCAAAGGG CCATGGACTA TTTCCCCAAG ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CACATGGTTT CTTCCTTTTG CATTGAGAAC TGTCATCGGG TGGAGTCACT GTCCCTGGGG 
TTTCTCCATA ACATGCCCAA GGAGGAAGAG GAGGAGGAAA AGGAAGGCCG ACACCTTGAT 
ATGGTGCAGT GTGTCCTCCC AAGCTCCTCT CATGCTGCCT GTTCTCATGG ATTGGTGAAC 
AGCCACCTCA CTTCCAGTTT TTGCCGGGGC CTCTTTTCAG TTCTGAGCAC CAGCCAGAGT 
CTAACTGAAT TGGACCTCAG TGACAATTCT CTGGGGGACC CAGGGATGAG AGTGTTGTGT 
GAAACGCTCC AGCATCCTGG CTGTAACATT CGGAGATTGT GGTTGGGGCG CTGTGGCCTC 
TCGCATGAGT GCTGCTTCGA CATCTCCTTG GTCCTCAGCA GCAACCAGAA GCTGGTGGAG 
CTGGACCTGA GTGACAACGC CCTCGGTGAC TTCGGAATCA GACTTCTGTG TGTGGGACTG 
AAGCACCTGT TGTGCAATCT GAAGAAGCTC TGGTTGGTCA GCTGCTGCCT CACATCAGCA 
TGTTGTCAGG ATCTTGCATC AGTATTGAGC ACCAGCCATT CCCTGACCAG ACTCTATGTG 
GGGGAGAATG CCTTGGGAGA CTCAGGAGTC GCAATTTTAT GTGAAAAAGC CAAGAATCCA 
CAGTGTAACC TGCAGAAACT GGGGTTGGTG AATTCTGGCC TTACGTCAGT CTGTTGTTCA 
GCTTTGTCCT CGGTACTCAG CACTAATCAG AATCTCACGC ACCTTTACCT GCGAGGCAAC 
ACTCTCGGAG ACAAGGGGAT CAAACTACTC TGTGAGGGAC TCTTGCACCC CGACTGCAAG 
CTTCAGGTGT TGGAATTAGA CAACTGCAAC CTCACGTCAC ACTGCTGCTG GGATCTTTCC 
ACACTTCTGA CCTCCAGCCA GAGCCTGCGA AAGCTGAGCC TGGGCAACAA TGACCTGGGC 
GACCTGGGGG TCATGATGTT CTGTGAAGTG CTGAAACAGC AGAGCTGCCT CCTGCAGAAC 
CTGGGGTTGT CTGAAATGTA TTTCAATTAT GAGACAAAAA GTGCGTTAGA AACACTTCAA 
GAAGAAAAGC CTGAGCTGAC CGTCGTCTTT GAGCCTTCTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_004895.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004886.3 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 1, mRNA.

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CloneID	OHu34986
Clone ID Related Accession (Same CDS sequence)	XM_017000181.1 , XM_017000182.1 , XM_011544048.2 , NM_004895.5
Accession Version	NM_004895.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104335.2, AF427617.1 and AF410477.1. On Dec 17, 2019 this sequence version replaced NM_004895.4. Transcript Variant: This variant (1) represents the same transcript, but uses an upstream translation start codon, compared to variant 6. Variants 1 and 6 represent the same transcript but use different translation start sites since both sites are likely used. The encoded isoform (a) uses a less conserved upstream translation start codon and has a slightly longer N-terminus, compared to isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in primates, rat, kangaroo rat, dolphin, cow, cat, elephant, tenrec and armadillo. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 2 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF468522.1, BC143362.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004886.3
CDS	764..3874
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	NM_004895.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004895.5

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_017000181.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855670.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu34986
Clone ID Related Accession (Same CDS sequence)	XM_017000181.1 , XM_017000182.1 , XM_011544048.2 , NM_004895.5
Accession Version	XM_017000181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855670.1
CDS	363..3473
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_017000181.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000181.1

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_017000182.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855671.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu34986
Clone ID Related Accession (Same CDS sequence)	XM_017000181.1 , XM_017000182.1 , XM_011544048.2 , NM_004895.5
Accession Version	XM_017000182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855671.1
CDS	308..3418
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_017000182.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000182.1

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_011544048.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542350.1 Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

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CloneID	OHu34986
Clone ID Related Accession (Same CDS sequence)	XM_017000181.1 , XM_017000182.1 , XM_011544048.2 , NM_004895.5
Accession Version	XM_011544048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542350.1
CDS	886..3996
Translation	MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Target ORF information:

RefSeq Version	XM_011544048.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544048.2

ORF Insert Sequence:

ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG 
GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC 
CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC 
TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT 
GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA 
CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC 
AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG 
AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG 
CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG 
AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC 
ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG 
TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC 
TGTCGAGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC 
CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC 
ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT 
GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG 
CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC 
TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG 
TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG 
GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT 
GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC 
GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC 
GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG 
AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT 
GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC 
ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG 
GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC 
CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC 
TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC 
TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG 
CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG 
GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA 
ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC 
CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC 
CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG 
GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC 
CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG 
TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT 
GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG 
GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG 
GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA 
TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC 
TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG 
AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT 
TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA 
GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC 
TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC 
CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG 
CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA 
CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NLRP3
Entrez Gene ID	114548
Full Name	NLR family pyrin domain containing 3
Synonyms	AGTAVPRL,AII,AVP,C1orf7,CIAS1,CLR1.1,DFNA34,FCAS,FCAS1,FCU,MWS,NALP3,PYPAF1
General protein information	Preferred Names NLR family pyrin domain containing 3 Names NACHT, LRR and PYD domains-containing protein 3 NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3 NACHT, LRR and PYD containing protein 3 PYRIN-containing APAF1-like protein 1 caterpiller protein 1.1 cold autoinflammatory syndrome 1 protein cold-induced autoinflammatory syndrome 1 protein cryopyrin nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q44
Summary	This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008].
Disorder MIM:	606416
Disorder Html:	Familial cold-induced inflammatory syndrome 1, 120100 (3); Muckle-Wells syndrome, 191900 (3); CINCA syndrome, 607115 (3); Deafness, autosomal dominant 34, with or without inflammation, 617772 (3)

Homo sapiens (human)	NLRP3	NP_004886.3
	NLRP3	NP_001107823.1
Xenopus tropicalis (tropical clawed frog)	LOC100487412	XP_002938247.2
Xenopus tropicalis (tropical clawed frog)	LOC101730849	XP_004917660.1
Xenopus tropicalis (tropical clawed frog)	LOC100488807	XP_002943171.1
Rattus norvegicus (Norway rat)	Nlrp3	NP_001178571.1
Xenopus tropicalis (tropical clawed frog)	LOC101731251	XP_004911151.1
Xenopus tropicalis (tropical clawed frog)	LOC100489137	XP_002943173.1
Canis lupus familiaris (dog)	NLRP3	XP_848377.2
Mus musculus (house mouse)	Nlrp3	NP_665826.1
Xenopus tropicalis (tropical clawed frog)	LOC101730480	XP_004917655.1
Xenopus tropicalis (tropical clawed frog)	LOC100488969	XP_002943172.2
Pan troglodytes (chimpanzee)	NLRP3	XP_003949788.1
Xenopus tropicalis (tropical clawed frog)	LOC100491706	XP_002944439.2
Xenopus tropicalis (tropical clawed frog)	LOC100495083	XP_002940532.2
Xenopus tropicalis (tropical clawed frog)	LOC100486737	XP_002937318.2
Xenopus tropicalis (tropical clawed frog)	LOC101730955	XP_004917628.1
Xenopus tropicalis (tropical clawed frog)	LOC101730774	XP_004917659.1
Bos taurus (cattle)	NLRP3	NP_001095689.1
Gallus gallus (chicken)	NLRP3	XP_001233262.3
Xenopus tropicalis (tropical clawed frog)	LOC100497524	XP_002945565.2
Xenopus tropicalis (tropical clawed frog)	LOC100486578	XP_002937317.2
Xenopus tropicalis (tropical clawed frog)	LOC100493406	XP_004917630.1
Xenopus tropicalis (tropical clawed frog)	LOC100488643	XP_002943170.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NLRP3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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