CHRNB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNB1
Entrez Gene ID	1140
Full Name	cholinergic receptor nicotinic beta 1 subunit
Synonyms	ACHRB,CHRNB,CMS1D,CMS2A,CMS2C,SCCMS
General protein information	Preferred Names cholinergic receptor nicotinic beta 1 subunit Names acetylcholine receptor subunit beta acetylcholine receptor, nicotinic, beta 1 (muscle) cholinergic receptor, nicotinic beta 1 cholinergic receptor, nicotinic, beta 1 (muscle) cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one beta, one gamma, and one delta subunit. This gene encodes the beta subunit of the acetylcholine receptor. The acetylcholine receptor changes conformation upon acetylcholine binding leading to the opening of an ion-conducting channel across the plasma membrane. Mutations in this gene are associated with slow-channel congenital myasthenic syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	100710
Disorder Html:	Myasthenic syndrome, congenital, 2A, slow-channel, 616313 (3); ?Myasthenic syndrome, congenital, 2C, associated with acetylcholine receptor deficiency, 616314 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000747.3	NP_000738.2	acetylcholine receptor subunit beta precursor
NM_000747.2	NP_000738.2	acetylcholine receptor subunit beta precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHRNB1	XP_511298.3
Danio rerio (zebrafish)	chrnb1	NP_001240739.1
Canis lupus familiaris (dog)	CHRNB1	XP_546591.2
Bos taurus (cattle)	CHRNB1	NP_776941.1
Mus musculus (house mouse)	Chrnb1	NP_033731.3
Homo sapiens (human)	CHRNB1	NP_000738.2
	CHRNB1	XP_001108848.2
Rattus norvegicus (Norway rat)	Chrnb1	NP_036660.1

Gene Ontology
Bibliography

Related articles in PubMed

Cellular protein and mRNA expression of ?1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta.
Aishah A, Hinton T, Machaalani R
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience589-16(2017 May)

Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating.
Shen XM, Okuno T, Milone M, Otsuka K, Takahashi K, Komaki H, Giles E, Ohno K, Engel AG
Human mutation37(10)1051-9(2016 10)

Determinants in the ? and ? subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor.
Rudell JC, Borges LS, Rudell JB, Beck KA, Ferns MJ
The Journal of biological chemistry289(1)203-14(2014 Jan)

Association of single nucleotide polymorphisms of nicotinic acetylcholine receptor subunits with cervical neoplasia.
Calleja-Macias I, Osann K, Remedios-Chan M, Barrera-Saldana HA, Illades-Aguiar B, Anton-Culver H, Chikova AK, Grando SA, Bernard HU
Life sciences91(21-22)1099-102(2012 Nov)

Resequencing of nicotinic acetylcholine receptor genes and association of common and rare variants with the Fagerstr?m test for nicotine dependence.
Wessel J, McDonald SM, Hinds DA, Stokowski RP, Javitz HS, Kennemer M, Krasnow R, Dirks W, Hardin J, Pitts SJ, Michel M, Jack L, Ballinger DG, McClure JB, Swan GE, Bergen AW
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology35(12)2392-402(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reports on the presence of the CHRNB1 at the cellular level in human skeletal muscle and placenta and that mRNA expression mirrors that of protein within cell types.
Title: Cellular protein and mRNA expression of ?1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta.

Study traced the cause of congenital myasthenia syndrome in unrelated patients with dominant missense mutations in the M2 domain of AChR. A valine residue is replaced by a smaller alanine either in the epsilon subunit or an equivalent position in the beta one. Also, each valine in the valine ring was found to contribute to channel kinetics equally, and the valine ring has bee optimized during evolution to govern channe...
Title: Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating.

These findings identify novel Golgi retention signals in the beta and delta subunit loops that regulate surface trafficking of assembled AChR and may help prevent surface expression of unassembled subunits.
Title: Determinants in the ? and ? subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor.

Single nucleotide polymorphisms , rs55633891 (alpha9, C>T) and rs17856697 (beta1, A>G), did not exhibit a significant trend to be associated with cervical lesions.
Title: Association of single nucleotide polymorphisms of nicotinic acetylcholine receptor subunits with cervical neoplasia.

Observational study of gene-disease association. (HuGE Navigator)
Title: Resequencing of nicotinic acetylcholine receptor genes and association of common and rare variants with the Fagerstr?m test for nicotine dependence.

The following CHRNB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19287	NM_000747.3 Latest version!	Homo sapiens cholinergic receptor nicotinic beta 1 subunit (CHRNB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19287	NM_000747.2	Homo sapiens cholinergic receptor nicotinic beta 1 subunit (CHRNB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNB1 ( NM_000747.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000738.2 Homo sapiens cholinergic receptor nicotinic beta 1 subunit (CHRNB1), mRNA.

Price & Availability↑

CloneID	OHu19287
Clone ID Related Accession (Same CDS sequence)	NM_000747.3 , NM_000747.2
Accession Version	NM_000747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit beta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC113189.11, BC023553.2 and BC011371.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000747.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023553.2, AK308106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306071.7/ ENSP00000304290.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCCCAG GGGCTCTGCT GATGCTGCTG GGGGCGCTGG GGGCGCCGCT CGCCCCAGGC 
GTCCGCGGCT CGGAGGCGGA GGGTCGACTC CGGGAGAAAC TTTTCTCTGG CTATGATAGC 
TCCGTGCGGC CAGCGCGGGA GGTGGGAGAC CGTGTCAGGG TCAGCGTTGG TCTCATCCTG 
GCGCAACTCA TCAGCCTGAA CGAGAAGGAT GAAGAGATGA GCACAAAGGT GTACTTAGAC 
CTGGAGTGGA CTGACTACAG GCTGAGCTGG GACCCTGCGG AGCACGACGG CATCGATTCG 
CTCCGCATCA CGGCGGAATC CGTGTGGCTC CCTGACGTGG TGCTACTGAA CAACAATGAT 
GGGAATTTTG ACGTGGCTCT GGACATTAGC GTCGTGGTGT CCTCCGACGG CTCCGTGCGT 
TGGCAACCCC CGGGCATCTA TCGCAGCAGC TGCAGCATCC AGGTCACCTA CTTCCCCTTC 
GACTGGCAGA ATTGCACTAT GGTGTTCAGC TCCTACAGCT ACGACAGCTC GGAGGTCAGC 
CTGCAGACAG GCCTGGGTCC TGACGGGCAA GGGCATCAGG AAATCCACAT TCATGAAGGG 
ACTTTCATTG AGAATGGCCA GTGGGAGATT ATCCACAAGC CCTCTCGGCT AATCCAGCCT 
CCAGGCGATC CTAGGGGAGG GAGGGAAGGA CAGCGCCAGG AAGTCATCTT CTACCTCATC 
ATCCGCCGCA AGCCTCTCTT CTACCTGGTC AACGTCATTG CCCCATGCAT CCTCATCACT 
CTTCTGGCCA TCTTCGTCTT CTACCTGCCA CCAGATGCAG GAGAGAAGAT GGGGCTCTCA 
ATCTTTGCCC TGCTGACCCT TACTGTGTTC CTGCTGCTGC TGGCTGACAA AGTACCTGAG 
ACCTCACTAT CAGTACCCAT TATTATCAAG TACCTCATGT TTACCATGGT CCTCGTCACC 
TTCTCAGTCA TCCTTAGTGT CGTGGTTCTC AACCTGCACC ACCGCTCACC CCACACCCAC 
CAAATGCCCC TTTGGGTCCG TCAGATCTTC ATTCACAAAC TTCCGCTGTA CCTGCGTCTA 
AAAAGGCCCA AACCCGAGAG AGACCTGATG CCGGAGCCCC CTCACTGTTC TTCTCCAGGA 
AGTGGCTGGG GTCGGGGAAC AGATGAATAT TTCATCCGGA AGCCGCCAAG TGATTTTCTC 
TTCCCCAAAC CCAATAGGTT CCAGCCTGAA CTGTCTGCCC CTGATCTGCG GCGATTTATC 
GATGGTCCAA ACCGGGCTGT GGCCCTGCTT CCGGAGCTAC GGGAGGTCGT CTCCTCTATC 
AGCTACATCG CTCGACAGCT GCAGGAACAG GAGGACCACG ATGCGCTGAA GGAGGACTGG 
CAGTTTGTGG CCATGGTAGT GGACCGCCTC TTCCTGTGGA CTTTCATCAT CTTCACCAGC 
GTTGGGACCC TAGTCATCTT CCTGGACGCC ACGTACCACT TGCCCCCTCC AGACCCCTTT 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000738.2
CDS	68..1573
Translation	MTPGALLMLLGALGAPLAPGVRGSEAEGRLREKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKDEEMSTKVYLDLEWTDYRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDISVVVSSDGSVRWQPPGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFIENGQWEIIHKPSRLIQPPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLSIFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQMPLWVRQIFIHKLPLYLRLKRPKPERDLMPEPPHCSSPGSGWGRGTDEYFIRKPPSDFLFPKPNRFQPELSAPDLRRFIDGPNRAVALLPELREVVSSISYIARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFTSVGTLVIFLDATYHLPPPDPFP

Target ORF information:

RefSeq Version	NM_000747.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 1 subunit (CHRNB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000747.3

ORF Insert Sequence:

ATGACCCCAG GGGCTCTGCT GATGCTGCTG GGGGCGCTGG GGGCGCCGCT CGCCCCAGGC 
GTCCGCGGCT CGGAGGCGGA GGGTCGACTC CGGGAGAAAC TTTTCTCTGG CTATGATAGC 
TCCGTGCGGC CAGCGCGGGA GGTGGGAGAC CGTGTCAGGG TCAGCGTTGG TCTCATCCTG 
GCGCAACTCA TCAGCCTGAA CGAGAAGGAT GAAGAGATGA GCACAAAGGT GTACTTAGAC 
CTGGAGTGGA CTGACTACAG GCTGAGCTGG GACCCTGCGG AGCACGACGG CATCGATTCG 
CTCCGCATCA CGGCGGAATC CGTGTGGCTC CCTGACGTGG TGCTACTGAA CAACAATGAT 
GGGAATTTTG ACGTGGCTCT GGACATTAGC GTCGTGGTGT CCTCCGACGG CTCCGTGCGT 
TGGCAACCCC CGGGCATCTA TCGCAGCAGC TGCAGCATCC AGGTCACCTA CTTCCCCTTC 
GACTGGCAGA ATTGCACTAT GGTGTTCAGC TCCTACAGCT ACGACAGCTC GGAGGTCAGC 
CTGCAGACAG GCCTGGGTCC TGACGGGCAA GGGCATCAGG AAATCCACAT TCATGAAGGG 
ACTTTCATTG AGAATGGCCA GTGGGAGATT ATCCACAAGC CCTCTCGGCT AATCCAGCCT 
CCAGGCGATC CTAGGGGAGG GAGGGAAGGA CAGCGCCAGG AAGTCATCTT CTACCTCATC 
ATCCGCCGCA AGCCTCTCTT CTACCTGGTC AACGTCATTG CCCCATGCAT CCTCATCACT 
CTTCTGGCCA TCTTCGTCTT CTACCTGCCA CCAGATGCAG GAGAGAAGAT GGGGCTCTCA 
ATCTTTGCCC TGCTGACCCT TACTGTGTTC CTGCTGCTGC TGGCTGACAA AGTACCTGAG 
ACCTCACTAT CAGTACCCAT TATTATCAAG TACCTCATGT TTACCATGGT CCTCGTCACC 
TTCTCAGTCA TCCTTAGTGT CGTGGTTCTC AACCTGCACC ACCGCTCACC CCACACCCAC 
CAAATGCCCC TTTGGGTCCG TCAGATCTTC ATTCACAAAC TTCCGCTGTA CCTGCGTCTA 
AAAAGGCCCA AACCCGAGAG AGACCTGATG CCGGAGCCCC CTCACTGTTC TTCTCCAGGA 
AGTGGCTGGG GTCGGGGAAC AGATGAATAT TTCATCCGGA AGCCGCCAAG TGATTTTCTC 
TTCCCCAAAC CCAATAGGTT CCAGCCTGAA CTGTCTGCCC CTGATCTGCG GCGATTTATC 
GATGGTCCAA ACCGGGCTGT GGCCCTGCTT CCGGAGCTAC GGGAGGTCGT CTCCTCTATC 
AGCTACATCG CTCGACAGCT GCAGGAACAG GAGGACCACG ATGCGCTGAA GGAGGACTGG 
CAGTTTGTGG CCATGGTAGT GGACCGCCTC TTCCTGTGGA CTTTCATCAT CTTCACCAGC 
GTTGGGACCC TAGTCATCTT CCTGGACGCC ACGTACCACT TGCCCCCTCC AGACCCCTTT 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNB1 ( NM_000747.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000738.2 Homo sapiens cholinergic receptor nicotinic beta 1 subunit (CHRNB1), mRNA.

Price & Availability↑

CloneID	OHu19287
Clone ID Related Accession (Same CDS sequence)	NM_000747.3 , NM_000747.2
Accession Version	NM_000747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-12
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit beta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC023553.2 and BC011371.1. This sequence is a reference standard in the RefSeqGene project. On Jan 26, 2004 this sequence version replaced NM_000747.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023553.2, BC011371.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG GGGCTCTGCT GATGCTGCTG GGGGCGCTGG GGGCGCCGCT CGCCCCAGGC 
GTCCGCGGCT CGGAGGCGGA GGGTCGACTC CGGGAGAAAC TTTTCTCTGG CTATGATAGC 
TCCGTGCGGC CAGCGCGGGA GGTGGGAGAC CGTGTCAGGG TCAGCGTTGG TCTCATCCTG 
GCGCAACTCA TCAGCCTGAA CGAGAAGGAT GAAGAGATGA GCACAAAGGT GTACTTAGAC 
CTGGAGTGGA CTGACTACAG GCTGAGCTGG GACCCTGCGG AGCACGACGG CATCGATTCG 
CTCCGCATCA CGGCGGAATC CGTGTGGCTC CCTGACGTGG TGCTACTGAA CAACAATGAT 
GGGAATTTTG ACGTGGCTCT GGACATTAGC GTCGTGGTGT CCTCCGACGG CTCCGTGCGT 
TGGCAACCCC CGGGCATCTA TCGCAGCAGC TGCAGCATCC AGGTCACCTA CTTCCCCTTC 
GACTGGCAGA ATTGCACTAT GGTGTTCAGC TCCTACAGCT ACGACAGCTC GGAGGTCAGC 
CTGCAGACAG GCCTGGGTCC TGACGGGCAA GGGCATCAGG AAATCCACAT TCATGAAGGG 
ACTTTCATTG AGAATGGCCA GTGGGAGATT ATCCACAAGC CCTCTCGGCT AATCCAGCCT 
CCAGGCGATC CTAGGGGAGG GAGGGAAGGA CAGCGCCAGG AAGTCATCTT CTACCTCATC 
ATCCGCCGCA AGCCTCTCTT CTACCTGGTC AACGTCATTG CCCCATGCAT CCTCATCACT 
CTTCTGGCCA TCTTCGTCTT CTACCTGCCA CCAGATGCAG GAGAGAAGAT GGGGCTCTCA 
ATCTTTGCCC TGCTGACCCT TACTGTGTTC CTGCTGCTGC TGGCTGACAA AGTACCTGAG 
ACCTCACTAT CAGTACCCAT TATTATCAAG TACCTCATGT TTACCATGGT CCTCGTCACC 
TTCTCAGTCA TCCTTAGTGT CGTGGTTCTC AACCTGCACC ACCGCTCACC CCACACCCAC 
CAAATGCCCC TTTGGGTCCG TCAGATCTTC ATTCACAAAC TTCCGCTGTA CCTGCGTCTA 
AAAAGGCCCA AACCCGAGAG AGACCTGATG CCGGAGCCCC CTCACTGTTC TTCTCCAGGA 
AGTGGCTGGG GTCGGGGAAC AGATGAATAT TTCATCCGGA AGCCGCCAAG TGATTTTCTC 
TTCCCCAAAC CCAATAGGTT CCAGCCTGAA CTGTCTGCCC CTGATCTGCG GCGATTTATC 
GATGGTCCAA ACCGGGCTGT GGCCCTGCTT CCGGAGCTAC GGGAGGTCGT CTCCTCTATC 
AGCTACATCG CTCGACAGCT GCAGGAACAG GAGGACCACG ATGCGCTGAA GGAGGACTGG 
CAGTTTGTGG CCATGGTAGT GGACCGCCTC TTCCTGTGGA CTTTCATCAT CTTCACCAGC 
GTTGGGACCC TAGTCATCTT CCTGGACGCC ACGTACCACT TGCCCCCTCC AGACCCCTTT 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000738.2
CDS	42..1547
Translation	MTPGALLMLLGALGAPLAPGVRGSEAEGRLREKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKDEEMSTKVYLDLEWTDYRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDISVVVSSDGSVRWQPPGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFIENGQWEIIHKPSRLIQPPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLSIFALLTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQMPLWVRQIFIHKLPLYLRLKRPKPERDLMPEPPHCSSPGSGWGRGTDEYFIRKPPSDFLFPKPNRFQPELSAPDLRRFIDGPNRAVALLPELREVVSSISYIARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFTSVGTLVIFLDATYHLPPPDPFP

Target ORF information:

RefSeq Version	NM_000747.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 1 subunit (CHRNB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000747.2

ORF Insert Sequence:

ATGACCCCAG GGGCTCTGCT GATGCTGCTG GGGGCGCTGG GGGCGCCGCT CGCCCCAGGC 
GTCCGCGGCT CGGAGGCGGA GGGTCGACTC CGGGAGAAAC TTTTCTCTGG CTATGATAGC 
TCCGTGCGGC CAGCGCGGGA GGTGGGAGAC CGTGTCAGGG TCAGCGTTGG TCTCATCCTG 
GCGCAACTCA TCAGCCTGAA CGAGAAGGAT GAAGAGATGA GCACAAAGGT GTACTTAGAC 
CTGGAGTGGA CTGACTACAG GCTGAGCTGG GACCCTGCGG AGCACGACGG CATCGATTCG 
CTCCGCATCA CGGCGGAATC CGTGTGGCTC CCTGACGTGG TGCTACTGAA CAACAATGAT 
GGGAATTTTG ACGTGGCTCT GGACATTAGC GTCGTGGTGT CCTCCGACGG CTCCGTGCGT 
TGGCAACCCC CGGGCATCTA TCGCAGCAGC TGCAGCATCC AGGTCACCTA CTTCCCCTTC 
GACTGGCAGA ATTGCACTAT GGTGTTCAGC TCCTACAGCT ACGACAGCTC GGAGGTCAGC 
CTGCAGACAG GCCTGGGTCC TGACGGGCAA GGGCATCAGG AAATCCACAT TCATGAAGGG 
ACTTTCATTG AGAATGGCCA GTGGGAGATT ATCCACAAGC CCTCTCGGCT AATCCAGCCT 
CCAGGCGATC CTAGGGGAGG GAGGGAAGGA CAGCGCCAGG AAGTCATCTT CTACCTCATC 
ATCCGCCGCA AGCCTCTCTT CTACCTGGTC AACGTCATTG CCCCATGCAT CCTCATCACT 
CTTCTGGCCA TCTTCGTCTT CTACCTGCCA CCAGATGCAG GAGAGAAGAT GGGGCTCTCA 
ATCTTTGCCC TGCTGACCCT TACTGTGTTC CTGCTGCTGC TGGCTGACAA AGTACCTGAG 
ACCTCACTAT CAGTACCCAT TATTATCAAG TACCTCATGT TTACCATGGT CCTCGTCACC 
TTCTCAGTCA TCCTTAGTGT CGTGGTTCTC AACCTGCACC ACCGCTCACC CCACACCCAC 
CAAATGCCCC TTTGGGTCCG TCAGATCTTC ATTCACAAAC TTCCGCTGTA CCTGCGTCTA 
AAAAGGCCCA AACCCGAGAG AGACCTGATG CCGGAGCCCC CTCACTGTTC TTCTCCAGGA 
AGTGGCTGGG GTCGGGGAAC AGATGAATAT TTCATCCGGA AGCCGCCAAG TGATTTTCTC 
TTCCCCAAAC CCAATAGGTT CCAGCCTGAA CTGTCTGCCC CTGATCTGCG GCGATTTATC 
GATGGTCCAA ACCGGGCTGT GGCCCTGCTT CCGGAGCTAC GGGAGGTCGT CTCCTCTATC 
AGCTACATCG CTCGACAGCT GCAGGAACAG GAGGACCACG ATGCGCTGAA GGAGGACTGG 
CAGTTTGTGG CCATGGTAGT GGACCGCCTC TTCCTGTGGA CTTTCATCAT CTTCACCAGC 
GTTGGGACCC TAGTCATCTT CCTGGACGCC ACGTACCACT TGCCCCCTCC AGACCCCTTT 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular protein and mRNA expression of ?1 nicotinic acetylcholine receptor (nAChR) subunit in brain, skeletal muscle and placenta.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience589-16(2017 May)
Aishah A,Hinton T,Machaalani R

Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating.
Human mutation37(10)1051-9(2016 10)
Shen XM,Okuno T,Milone M,Otsuka K,Takahashi K,Komaki H,Giles E,Ohno K,Engel AG

Determinants in the ? and ? subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor.
The Journal of biological chemistry289(1)203-14(2014 Jan)
Rudell JC,Borges LS,Rudell JB,Beck KA,Ferns MJ

Association of single nucleotide polymorphisms of nicotinic acetylcholine receptor subunits with cervical neoplasia.
Life sciences91(21-22)1099-102(2012 Nov)
Calleja-Macias I,Osann K,Remedios-Chan M,Barrera-Saldana HA,Illades-Aguiar B,Anton-Culver H,Chikova AK,Grando SA,Bernard HU

Resequencing of nicotinic acetylcholine receptor genes and association of common and rare variants with the Fagerstr?m test for nicotine dependence.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology35(12)2392-402(2010 Nov)
Wessel J,McDonald SM,Hinds DA,Stokowski RP,Javitz HS,Kennemer M,Krasnow R,Dirks W,Hardin J,Pitts SJ,Michel M,Jack L,Ballinger DG,McClure JB,Swan GE,Bergen AW

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CHRNB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報