DDX42 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX42
Entrez Gene ID	11325
Full Name	DEAD-box helicase 42
Synonyms	DDX42P,RHELP,RNAHP,SF3B8,SF3b125
General protein information	Preferred Names DEAD-box helicase 42 Names ATP-dependent RNA helicase DDX42 DEAD (Asp-Glu-Ala-Asp) box helicase 42 DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 SF3b DEAD box protein splicing factor 3B-associated 125 kDa protein splicing factor 3b, subunit 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.3
Summary	This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	613369

mRNA and Protein(s)

mRNA	Protein	Name
XM_006721657.1	XP_006721720.1	ATP-dependent RNA helicase DDX42 isoform X1
NM_203499.2	NP_987095.1	ATP-dependent RNA helicase DDX42
XM_017024111.1	XP_016879600.1	ATP-dependent RNA helicase DDX42 isoform X1
XM_024450558.1	XP_024306326.1	ATP-dependent RNA helicase DDX42 isoform X2
NM_007372.3	NP_031398.2	ATP-dependent RNA helicase DDX42
XM_017024112.1	XP_016879601.1	ATP-dependent RNA helicase DDX42 isoform X2
XM_011524259.2	XP_011522561.1	ATP-dependent RNA helicase DDX42 isoform X3
NM_203499.3	NP_987095.1	ATP-dependent RNA helicase DDX42

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx42	NP_082350.3
Rattus norvegicus (Norway rat)	Ddx42	NP_001100529.1
Caenorhabditis elegans (roundworm)	C46F11.4	NP_497743.1
Canis lupus familiaris (dog)	DDX42	XP_537598.3
Danio rerio (zebrafish)	ddx42	NP_001032894.2
Homo sapiens (human)	DDX42	NP_031398.2
Pan troglodytes (chimpanzee)	DDX42	XP_001147880.1
Bos taurus (cattle)	DDX42	XP_003587506.2
Arabidopsis thaliana (thale cress)	AT2G47330	NP_566099.1
Xenopus tropicalis (tropical clawed frog)	ddx42	XP_004918655.1
	DDX42	XP_001116381.1
Drosophila melanogaster (fruit fly)	CG6418	NP_648413.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012523	XP_306246.4
Gallus gallus (chicken)	DDX42	NP_001026097.1
Oryza sativa (rice)	Os03g0308500	NP_001049904.1

Gene Ontology
Bibliography

Related articles in PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The DEAD box protein Ddx42p modulates the function of ASPP2, a stimulator of apoptosis.
Uhlmann-Schiffler H, Kiermayer S, Stahl H
Oncogene28(20)2065-73(2009 May)

Interferon antagonist function of Japanese encephalitis virus NS4A and its interaction with DEAD-box RNA helicase DDX42.
Lin CW, Cheng CW, Yang TC, Li SW, Cheng MH, Wan L, Lin YJ, Lai CH, Lin WY, Kao MC
Virus research137(1)49-55(2008 Oct)

Ddx42p--a human DEAD box protein with RNA chaperone activities.
Uhlmann-Schiffler H, Jalal C, Stahl H
Nucleic acids research34(1)10-22(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression of N-terminal DDX42 is able to overcome Japanese encephalitis virus-induced antagonism of IFN responses and so may be important for Japanese encephalitis virus pathogenesis
Title: Interferon antagonist function of Japanese encephalitis virus NS4A and its interaction with DEAD-box RNA helicase DDX42.

Ddx42p represents the first example of a human DEAD box protein possessing RNA helicase, protein displacement and RNA annealing activities.
Title: Ddx42p--a human DEAD box protein with RNA chaperone activities.

characterization of protein associated with the human 17S U2 snRNP and one of its stable subunits, SF3b; may facilitate the assembly of the 17S U2 snRNP
Title: Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.

The following DDX42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01556	NM_007372.3 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01556	NM_203499.3 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01556	XM_006721657.1 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01556	XM_017024111.1 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu64920	XM_011524259.2 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu86545	XM_024450558.1 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86545	XM_017024112.1 Latest version!	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu01556	NM_203499.2	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX42 ( NM_203499.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_987095.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01556
Clone ID Related Accession (Same CDS sequence)	XM_006721657.1 , NM_203499.2 , XM_017024111.1 , NM_007372.3 , NM_203499.3
Accession Version	NM_203499.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA440475.1, AB036090.1 and BC015505.1. On Jul 12, 2013 this sequence version replaced NM_203499.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.104354.1, SRR1803612.106913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_987095.1
CDS	205..3021
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	NM_203499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203499.2

ORF Insert Sequence:

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( NM_007372.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_031398.2 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01556
Clone ID Related Accession (Same CDS sequence)	XM_006721657.1 , NM_203499.2 , XM_017024111.1 , NM_007372.3 , NM_203499.3
Accession Version	NM_007372.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC015651.18, BC093081.1 and BC015505.1. On Jul 12, 2013 this sequence version replaced NM_007372.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC093081.1, SRR1803615.196748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031398.2
CDS	276..3092
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	NM_007372.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007372.3

ORF Insert Sequence:

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( NM_203499.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_987095.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 2, mRNA.

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CloneID	OHu01556
Clone ID Related Accession (Same CDS sequence)	XM_006721657.1 , NM_203499.2 , XM_017024111.1 , NM_007372.3 , NM_203499.3
Accession Version	NM_203499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC015651.18, DA440475.1, AB036090.1 and BC015505.1. On Nov 22, 2018 this sequence version replaced NM_203499.2. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.104354.1, SRR1803612.106913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389924.7/ ENSP00000374574.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_987095.1
CDS	224..3040
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	NM_203499.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203499.3

ORF Insert Sequence:

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( XM_006721657.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721720.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X1, mRNA.

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CloneID	OHu01556
Clone ID Related Accession (Same CDS sequence)	XM_006721657.1 , NM_203499.2 , XM_017024111.1 , NM_007372.3 , NM_203499.3
Accession Version	XM_006721657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721720.1
CDS	413..3229
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_006721657.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721657.1

ORF Insert Sequence:

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( XM_017024111.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879600.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X2, mRNA.

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CloneID	OHu01556
Clone ID Related Accession (Same CDS sequence)	XM_006721657.1 , NM_203499.2 , XM_017024111.1 , NM_007372.3 , NM_203499.3
Accession Version	XM_017024111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879600.1
CDS	512..3328
Translation	MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_017024111.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024111.1

ORF Insert Sequence:

ATGAACTGGA ATAAAGGTGG TCCTGGCACT AAGCGAGGAT TTGGCTTTGG AGGTTTTGCC 
ATCAGTGCTG GGAAAAAGGA GGAACCCAAA CTCCCACAGC AGTCCCACAG TGCCTTTGGG 
GCAACCAGCT CTTCTTCTGG ATTTGGAAAG TCAGCTCCAC CACAGCTTCC TTCTTTCTAC 
AAAATTGGAT CTAAGCGGGC CAACTTTGAT GAAGAAAATG CCTATTTTGA AGATGAGGAA 
GAAGATTCTA GCAACGTTGA TTTACCTTAC ATTCCTGCTG AAAACTCACC AACTCGCCAG 
CAATTCCATT CCAAGCCAGT AGATTCTGAC AGCGATGATG ATCCCTTGGA GGCATTCATG 
GCTGAAGTGG AGGATCAGGC AGCTAGAGAC ATGAAGAGGC TTGAAGAAAA GGACAAGGAA 
AGAAAAAACG TAAAGGGTAT TCGAGATGAC ATTGAAGAGG AAGATGACCA AGAAGCTTAT 
TTTCGATACA TGGCAGAAAA CCCAACTGCT GGTGTGGTTC AGGAGGAAGA GGAAGACAAT 
CTAGAATATG ATAGTGACGG AAATCCAATT GCACCTACCA AAAAAATCAT TGATCCTCTT 
CCCCCCATTG ATCATTCAGA GATTGACTAT CCACCATTTG AAAAAAACTT TTACAATGAG 
CATGAAGAGA TAACCAACCT CACTCCACAG CAGTTAATAG ATCTCCGGCA TAAGCTCAAT 
CTTCGGGTCT CTGGTGCTGC ACCTCCTAGA CCAGGAAGTA GCTTTGCTCA TTTTGGGTTT 
GACGAACAAC TTATGCACCA GATTCGGAAA TCTGAATACA CACAGCCCAC TCCAATACAG 
TGCCAGGGTG TGCCTGTGGC ATTAAGTGGT AGAGACATGA TTGGTATTGC CAAAACAGGT 
AGTGGGAAAA CTGCAGCCTT CATTTGGCCC ATGTTGATTC ATATAATGGA CCAGAAGGAG 
TTGGAACCAG GTGATGGACC AATTGCAGTG ATTGTGTGTC CTACCAGGGA GCTTTGCCAG 
CAGATCCATG CAGAATGTAA GCGGTTTGGA AAAGCATATA ATCTTCGATC AGTGGCCGTA 
TATGGAGGAG GGAGTATGTG GGAGCAGGCC AAGGCCCTTC AGGAGGGGGC AGAGATTGTT 
GTGTGTACCC CAGGTCGACT GATAGATCAT GTGAAAAAGA AAGCTACCAA TCTTCAAAGA 
GTCTCTTACC TTGTGTTTGA TGAAGCAGAT CGAATGTTTG ACATGGGATT TGAGTACCAA 
GTTCGATCCA TAGCAAGTCA TGTTCGTCCT GACAGGCAGA CTCTCTTATT TAGTGCAACT 
TTTCGGAAGA AGATTGAAAA GTTGGCCAGA GACATCCTGA TCGACCCTAT TCGAGTGGTG 
CAGGGAGATA TTGGAGAGGC AAATGAAGAT GTGACACAGA TTGTGGAGAT TCTCCATTCT 
GGACCTAGTA AATGGAACTG GCTTACCCGG CGTCTGGTAG AATTTACCTC TTCAGGGAGT 
GTCCTCCTCT TTGTTACTAA AAAAGCCAAT GCTGAAGAGC TAGCGAATAA CCTTAAACAG 
GAGGGTCATA ATCTTGGGCT GCTCCATGGG GATATGGATC AGAGTGAGAG AAACAAGGTC 
ATTTCAGACT TTAAGAAAAA GGACATCCCA GTCCTGGTGG CCACAGATGT TGCAGCCCGT 
GGTCTGGACA TTCCTTCAAT TAAGACTGTC ATTAACTATG ATGTGGCACG AGACATTGAT 
ACCCACACGC ATAGGATTGG CCGCACAGGA AGAGCGGGTG AGAAAGGTGT GGCCTATACC 
CTACTCACTC CCAAGGACAG CAATTTTGCT GGTGACCTGG TCCGGAACTT GGAAGGAGCC 
AATCAACACG TTTCTAAGGA ACTCCTAGAT CTGGCAATGC AGAATGCCTG GTTTCGGAAA 
TCTCGATTCA AAGGAGGGAA AGGAAAAAAG CTGAACATTG GTGGAGGAGG CCTAGGCTAC 
AGGGAGCGGC CTGGCCTGGG CTCTGAGAAC ATGGATCGAG GAAATAACAA TGTAATGAGC 
AATTATGAGG CCTACAAGCC TTCCACAGGA GCTATGGGAG ATCGACTAAC GGCAATGAAA 
GCAGCTTTCC AGTCACAGTA CAAGAGTCAC TTTGTTGCAG CCAGTTTAAG TAATCAGAAG 
GCTGGAAGTT CTGCTGCTGG GGCAAGTGGG TGGACTAGTG CAGGGAGCTT GAATTCTGTT 
CCAACTAACT CAGCACAACA GGGCCATAAC AGTCCTGACA GCCCCGTCAC CAGTGCCGCC 
AAGGGCATCC CAGGCTTTGG CAATACTGGC AACATCAGTG GTGCCCCTGT GACCTACCCG 
TCTGCCGGAG CCCAAGGAGT CAACAACACA GCTTCAGGGA ATAACAGCCG AGAAGGGACT 
GGGGGCAGCA ACGGGAAAAG AGAGAGATAT ACTGAGAACC GGGGCAGCAG CCGTCACAGT 
CACGGAGAGA CTGGCAATCG GCATAGCGAT AGTCCACGTC ACGGAGATGG TGGTCGCCAT 
GGAGATGGAT ACCGCCATCC AGAAAGCAGC AGCCGTCATA CTGATGGCCA TCGGCACGGG 
GAGAACAGAC ATGGAGGAAG CGCAGGCCGG CATGGGGAGA ACCGGGGTGC AAATGATGGT 
CGGAATGGGG AAAGCAGGAA AGAAGCTTTT AATCGTGAGA GCAAGATGGA GCCCAAGATG 
GAACCCAAAG TGGACAGCAG CAAGATGGAC AAGGTGGACA GCAAGACAGA TAAGACAGCT 
GACGGCTTTG CTGTCCCAGA GCCGCCTAAA CGCAAGAAAA GTCGATGGGA CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( XM_011524259.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522561.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu64920
Clone ID Related Accession (Same CDS sequence)	XM_011524259.2
Accession Version	XM_011524259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGA TTCGGAAATC TGAATACACA CAGCCCACTC CAATACAGTG CCAGGGTGTG 
CCTGTGGCAT TAAGTGGTAG AGACATGATT GGTATTGCCA AAACAGGTAG TGGGAAAACT 
GCAGCCTTCA TTTGGCCCAT GTTGATTCAT ATAATGGACC AGAAGGAGTT GGAACCAGGT 
GATGGACCAA TTGCAGTGAT TGTGTGTCCT ACCAGGGAGC TTTGCCAGCA GATCCATGCA 
GAATGTAAGC GGTTTGGAAA AGCATATAAT CTTCGATCAG TGGCCGTATA TGGAGGAGGG 
AGTATGTGGG AGCAGGCCAA GGCCCTTCAG GAGGGGGCAG AGATTGTTGT GTGTACCCCA 
GGTCGACTGA TAGATCATGT GAAAAAGAAA GCTACCAATC TTCAAAGAGT CTCTTACCTT 
GTGTTTGATG AAGCAGATCG AATGTTTGAC ATGGGATTTG AGTACCAAGT TCGATCCATA 
GCAAGTCATG TTCGTCCTGA CAGGCAGACT CTCTTATTTA GTGCAACTTT TCGGAAGAAG 
ATTGAAAAGT TGGCCAGAGA CATCCTGATC GACCCTATTC GAGTGGTGCA GGGAGATATT 
GGAGAGGCAA ATGAAGATGT GACACAGATT GTGGAGATTC TCCATTCTGG ACCTAGTAAA 
TGGAACTGGC TTACCCGGCG TCTGGTAGAA TTTACCTCTT CAGGGAGTGT CCTCCTCTTT 
GTTACTAAAA AAGCCAATGC TGAAGAGCTA GCGAATAACC TTAAACAGGA GGGTCATAAT 
CTTGGGCTGC TCCATGGGGA TATGGATCAG AGTGAGAGAA ACAAGGTCAT TTCAGACTTT 
AAGAAAAAGG ACATCCCAGT CCTGGTGGCC ACAGATGTTG CAGCCCGTGG TCTGGACATT 
CCTTCAATTA AGACTGTCAT TAACTATGAT GTGGCACGAG ACATTGATAC CCACACGCAT 
AGGATTGGCC GCACAGGAAG AGCGGGTGAG AAAGGTGTGG CCTATACCCT ACTCACTCCC 
AAGGACAGCA ATTTTGCTGG TGACCTGGTC CGGAACTTGG AAGGAGCCAA TCAACACGTT 
TCTAAGGAAC TCCTAGATCT GGCAATGCAG AATGCCTGGT TTCGGAAATC TCGATTCAAA 
GGAGGGAAAG GAAAAAAGCT GAACATTGGT GGAGGAGGCC TAGGCTACAG GGAGCGGCCT 
GGCCTGGGCT CTGAGAACAT GGATCGAGGA AATAACAATG TAATGAGCAA TTATGAGGCC 
TACAAGCCTT CCACAGGAGC TATGGGAGAT CGACTAACGG CAATGAAAGC AGCTTTCCAG 
TCACAGTACA AGAGTCACTT TGTTGCAGCC AGTTTAAGTA ATCAGAAGGC TGGAAGTTCT 
GCTGCTGGGG CAAGTGGGTG GACTAGTGCA GGGAGCTTGA ATTCTGTTCC AACTAACTCA 
GCACAACAGG GCCATAACAG TCCTGACAGC CCCGTCACCA GTGCCGCCAA GGGCATCCCA 
GGCTTTGGCA ATACTGGCAA CATCAGTGGT GCCCCTGTGA CCTACCCGTC TGCCGGAGCC 
CAAGGAGTCA ACAACACAGC TTCAGGGAAT AACAGCCGAG AAGGGACTGG GGGCAGCAAC 
GGGAAAAGAG AGAGATATAC TGAGAACCGG GGCAGCAGCC GTCACAGTCA CGGAGAGACT 
GGCAATCGGC ATAGCGATAG TCCACGTCAC GGAGATGGTG GTCGCCATGG AGATGGATAC 
CGCCATCCAG AAAGCAGCAG CCGTCATACT GATGGCCATC GGCACGGGGA GAACAGACAT 
GGAGGAAGCG CAGGCCGGCA TGGGGAGAAC CGGGGTGCAA ATGATGGTCG GAATGGGGAA 
AGCAGGAAAG AAGCTTTTAA TCGTGAGAGC AAGATGGAGC CCAAGATGGA ACCCAAAGTG 
GACAGCAGCA AGATGGACAA GGTGGACAGC AAGACAGATA AGACAGCTGA CGGCTTTGCT 
GTCCCAGAGC CGCCTAAACG CAAGAAAAGT CGATGGGACA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522561.1
CDS	1006..3030
Translation	MHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_011524259.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524259.2

ORF Insert Sequence:

ATGCACCAGA TTCGGAAATC TGAATACACA CAGCCCACTC CAATACAGTG CCAGGGTGTG 
CCTGTGGCAT TAAGTGGTAG AGACATGATT GGTATTGCCA AAACAGGTAG TGGGAAAACT 
GCAGCCTTCA TTTGGCCCAT GTTGATTCAT ATAATGGACC AGAAGGAGTT GGAACCAGGT 
GATGGACCAA TTGCAGTGAT TGTGTGTCCT ACCAGGGAGC TTTGCCAGCA GATCCATGCA 
GAATGTAAGC GGTTTGGAAA AGCATATAAT CTTCGATCAG TGGCCGTATA TGGAGGAGGG 
AGTATGTGGG AGCAGGCCAA GGCCCTTCAG GAGGGGGCAG AGATTGTTGT GTGTACCCCA 
GGTCGACTGA TAGATCATGT GAAAAAGAAA GCTACCAATC TTCAAAGAGT CTCTTACCTT 
GTGTTTGATG AAGCAGATCG AATGTTTGAC ATGGGATTTG AGTACCAAGT TCGATCCATA 
GCAAGTCATG TTCGTCCTGA CAGGCAGACT CTCTTATTTA GTGCAACTTT TCGGAAGAAG 
ATTGAAAAGT TGGCCAGAGA CATCCTGATC GACCCTATTC GAGTGGTGCA GGGAGATATT 
GGAGAGGCAA ATGAAGATGT GACACAGATT GTGGAGATTC TCCATTCTGG ACCTAGTAAA 
TGGAACTGGC TTACCCGGCG TCTGGTAGAA TTTACCTCTT CAGGGAGTGT CCTCCTCTTT 
GTTACTAAAA AAGCCAATGC TGAAGAGCTA GCGAATAACC TTAAACAGGA GGGTCATAAT 
CTTGGGCTGC TCCATGGGGA TATGGATCAG AGTGAGAGAA ACAAGGTCAT TTCAGACTTT 
AAGAAAAAGG ACATCCCAGT CCTGGTGGCC ACAGATGTTG CAGCCCGTGG TCTGGACATT 
CCTTCAATTA AGACTGTCAT TAACTATGAT GTGGCACGAG ACATTGATAC CCACACGCAT 
AGGATTGGCC GCACAGGAAG AGCGGGTGAG AAAGGTGTGG CCTATACCCT ACTCACTCCC 
AAGGACAGCA ATTTTGCTGG TGACCTGGTC CGGAACTTGG AAGGAGCCAA TCAACACGTT 
TCTAAGGAAC TCCTAGATCT GGCAATGCAG AATGCCTGGT TTCGGAAATC TCGATTCAAA 
GGAGGGAAAG GAAAAAAGCT GAACATTGGT GGAGGAGGCC TAGGCTACAG GGAGCGGCCT 
GGCCTGGGCT CTGAGAACAT GGATCGAGGA AATAACAATG TAATGAGCAA TTATGAGGCC 
TACAAGCCTT CCACAGGAGC TATGGGAGAT CGACTAACGG CAATGAAAGC AGCTTTCCAG 
TCACAGTACA AGAGTCACTT TGTTGCAGCC AGTTTAAGTA ATCAGAAGGC TGGAAGTTCT 
GCTGCTGGGG CAAGTGGGTG GACTAGTGCA GGGAGCTTGA ATTCTGTTCC AACTAACTCA 
GCACAACAGG GCCATAACAG TCCTGACAGC CCCGTCACCA GTGCCGCCAA GGGCATCCCA 
GGCTTTGGCA ATACTGGCAA CATCAGTGGT GCCCCTGTGA CCTACCCGTC TGCCGGAGCC 
CAAGGAGTCA ACAACACAGC TTCAGGGAAT AACAGCCGAG AAGGGACTGG GGGCAGCAAC 
GGGAAAAGAG AGAGATATAC TGAGAACCGG GGCAGCAGCC GTCACAGTCA CGGAGAGACT 
GGCAATCGGC ATAGCGATAG TCCACGTCAC GGAGATGGTG GTCGCCATGG AGATGGATAC 
CGCCATCCAG AAAGCAGCAG CCGTCATACT GATGGCCATC GGCACGGGGA GAACAGACAT 
GGAGGAAGCG CAGGCCGGCA TGGGGAGAAC CGGGGTGCAA ATGATGGTCG GAATGGGGAA 
AGCAGGAAAG AAGCTTTTAA TCGTGAGAGC AAGATGGAGC CCAAGATGGA ACCCAAAGTG 
GACAGCAGCA AGATGGACAA GGTGGACAGC AAGACAGATA AGACAGCTGA CGGCTTTGCT 
GTCCCAGAGC CGCCTAAACG CAAGAAAAGT CGATGGGACA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( XM_024450558.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306326.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu86545
Clone ID Related Accession (Same CDS sequence)	XM_024450558.1 , XM_017024112.1
Accession Version	XM_024450558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA ACCCAACTGC TGGTGTGGTT CAGGAGGAAG AGGAAGACAA TCTAGAATAT 
GATAGTGACG GAAATCCAAT TGCACCTACC AAAAAAATCA TTGATCCTCT TCCCCCCATT 
GATCATTCAG AGATTGACTA TCCACCATTT GAAAAAAACT TTTACAATGA GCATGAAGAG 
ATAACCAACC TCACTCCACA GCAGTTAATA GATCTCCGGC ATAAGCTCAA TCTTCGGGTC 
TCTGGTGCTG CACCTCCTAG ACCAGGAAGT AGCTTTGCTC ATTTTGGGTT TGACGAACAA 
CTTATGCACC AGATTCGGAA ATCTGAATAC ACACAGCCCA CTCCAATACA GTGCCAGGGT 
GTGCCTGTGG CATTAAGTGG TAGAGACATG ATTGGTATTG CCAAAACAGG TAGTGGGAAA 
ACTGCAGCCT TCATTTGGCC CATGTTGATT CATATAATGG ACCAGAAGGA GTTGGAACCA 
GGTGATGGAC CAATTGCAGT GATTGTGTGT CCTACCAGGG AGCTTTGCCA GCAGATCCAT 
GCAGAATGTA AGCGGTTTGG AAAAGCATAT AATCTTCGAT CAGTGGCCGT ATATGGAGGA 
GGGAGTATGT GGGAGCAGGC CAAGGCCCTT CAGGAGGGGG CAGAGATTGT TGTGTGTACC 
CCAGGTCGAC TGATAGATCA TGTGAAAAAG AAAGCTACCA ATCTTCAAAG AGTCTCTTAC 
CTTGTGTTTG ATGAAGCAGA TCGAATGTTT GACATGGGAT TTGAGTACCA AGTTCGATCC 
ATAGCAAGTC ATGTTCGTCC TGACAGGCAG ACTCTCTTAT TTAGTGCAAC TTTTCGGAAG 
AAGATTGAAA AGTTGGCCAG AGACATCCTG ATCGACCCTA TTCGAGTGGT GCAGGGAGAT 
ATTGGAGAGG CAAATGAAGA TGTGACACAG ATTGTGGAGA TTCTCCATTC TGGACCTAGT 
AAATGGAACT GGCTTACCCG GCGTCTGGTA GAATTTACCT CTTCAGGGAG TGTCCTCCTC 
TTTGTTACTA AAAAAGCCAA TGCTGAAGAG CTAGCGAATA ACCTTAAACA GGAGGGTCAT 
AATCTTGGGC TGCTCCATGG GGATATGGAT CAGAGTGAGA GAAACAAGGT CATTTCAGAC 
TTTAAGAAAA AGGACATCCC AGTCCTGGTG GCCACAGATG TTGCAGCCCG TGGTCTGGAC 
ATTCCTTCAA TTAAGACTGT CATTAACTAT GATGTGGCAC GAGACATTGA TACCCACACG 
CATAGGATTG GCCGCACAGG AAGAGCGGGT GAGAAAGGTG TGGCCTATAC CCTACTCACT 
CCCAAGGACA GCAATTTTGC TGGTGACCTG GTCCGGAACT TGGAAGGAGC CAATCAACAC 
GTTTCTAAGG AACTCCTAGA TCTGGCAATG CAGAATGCCT GGTTTCGGAA ATCTCGATTC 
AAAGGAGGGA AAGGAAAAAA GCTGAACATT GGTGGAGGAG GCCTAGGCTA CAGGGAGCGG 
CCTGGCCTGG GCTCTGAGAA CATGGATCGA GGAAATAACA ATGTAATGAG CAATTATGAG 
GCCTACAAGC CTTCCACAGG AGCTATGGGA GATCGACTAA CGGCAATGAA AGCAGCTTTC 
CAGTCACAGT ACAAGAGTCA CTTTGTTGCA GCCAGTTTAA GTAATCAGAA GGCTGGAAGT 
TCTGCTGCTG GGGCAAGTGG GTGGACTAGT GCAGGGAGCT TGAATTCTGT TCCAACTAAC 
TCAGCACAAC AGGGCCATAA CAGTCCTGAC AGCCCCGTCA CCAGTGCCGC CAAGGGCATC 
CCAGGCTTTG GCAATACTGG CAACATCAGT GGTGCCCCTG TGACCTACCC GTCTGCCGGA 
GCCCAAGGAG TCAACAACAC AGCTTCAGGG AATAACAGCC GAGAAGGGAC TGGGGGCAGC 
AACGGGAAAA GAGAGAGATA TACTGAGAAC CGGGGCAGCA GCCGTCACAG TCACGGAGAG 
ACTGGCAATC GGCATAGCGA TAGTCCACGT CACGGAGATG GTGGTCGCCA TGGAGATGGA 
TACCGCCATC CAGAAAGCAG CAGCCGTCAT ACTGATGGCC ATCGGCACGG GGAGAACAGA 
CATGGAGGAA GCGCAGGCCG GCATGGGGAG AACCGGGGTG CAAATGATGG TCGGAATGGG 
GAAAGCAGGA AAGAAGCTTT TAATCGTGAG AGCAAGATGG AGCCCAAGAT GGAACCCAAA 
GTGGACAGCA GCAAGATGGA CAAGGTGGAC AGCAAGACAG ATAAGACAGC TGACGGCTTT 
GCTGTCCCAG AGCCGCCTAA ACGCAAGAAA AGTCGATGGG ACAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306326.1
CDS	350..2677
Translation	MAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_024450558.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450558.1

ORF Insert Sequence:

ATGGCAGAAA ACCCAACTGC TGGTGTGGTT CAGGAGGAAG AGGAAGACAA TCTAGAATAT 
GATAGTGACG GAAATCCAAT TGCACCTACC AAAAAAATCA TTGATCCTCT TCCCCCCATT 
GATCATTCAG AGATTGACTA TCCACCATTT GAAAAAAACT TTTACAATGA GCATGAAGAG 
ATAACCAACC TCACTCCACA GCAGTTAATA GATCTCCGGC ATAAGCTCAA TCTTCGGGTC 
TCTGGTGCTG CACCTCCTAG ACCAGGAAGT AGCTTTGCTC ATTTTGGGTT TGACGAACAA 
CTTATGCACC AGATTCGGAA ATCTGAATAC ACACAGCCCA CTCCAATACA GTGCCAGGGT 
GTGCCTGTGG CATTAAGTGG TAGAGACATG ATTGGTATTG CCAAAACAGG TAGTGGGAAA 
ACTGCAGCCT TCATTTGGCC CATGTTGATT CATATAATGG ACCAGAAGGA GTTGGAACCA 
GGTGATGGAC CAATTGCAGT GATTGTGTGT CCTACCAGGG AGCTTTGCCA GCAGATCCAT 
GCAGAATGTA AGCGGTTTGG AAAAGCATAT AATCTTCGAT CAGTGGCCGT ATATGGAGGA 
GGGAGTATGT GGGAGCAGGC CAAGGCCCTT CAGGAGGGGG CAGAGATTGT TGTGTGTACC 
CCAGGTCGAC TGATAGATCA TGTGAAAAAG AAAGCTACCA ATCTTCAAAG AGTCTCTTAC 
CTTGTGTTTG ATGAAGCAGA TCGAATGTTT GACATGGGAT TTGAGTACCA AGTTCGATCC 
ATAGCAAGTC ATGTTCGTCC TGACAGGCAG ACTCTCTTAT TTAGTGCAAC TTTTCGGAAG 
AAGATTGAAA AGTTGGCCAG AGACATCCTG ATCGACCCTA TTCGAGTGGT GCAGGGAGAT 
ATTGGAGAGG CAAATGAAGA TGTGACACAG ATTGTGGAGA TTCTCCATTC TGGACCTAGT 
AAATGGAACT GGCTTACCCG GCGTCTGGTA GAATTTACCT CTTCAGGGAG TGTCCTCCTC 
TTTGTTACTA AAAAAGCCAA TGCTGAAGAG CTAGCGAATA ACCTTAAACA GGAGGGTCAT 
AATCTTGGGC TGCTCCATGG GGATATGGAT CAGAGTGAGA GAAACAAGGT CATTTCAGAC 
TTTAAGAAAA AGGACATCCC AGTCCTGGTG GCCACAGATG TTGCAGCCCG TGGTCTGGAC 
ATTCCTTCAA TTAAGACTGT CATTAACTAT GATGTGGCAC GAGACATTGA TACCCACACG 
CATAGGATTG GCCGCACAGG AAGAGCGGGT GAGAAAGGTG TGGCCTATAC CCTACTCACT 
CCCAAGGACA GCAATTTTGC TGGTGACCTG GTCCGGAACT TGGAAGGAGC CAATCAACAC 
GTTTCTAAGG AACTCCTAGA TCTGGCAATG CAGAATGCCT GGTTTCGGAA ATCTCGATTC 
AAAGGAGGGA AAGGAAAAAA GCTGAACATT GGTGGAGGAG GCCTAGGCTA CAGGGAGCGG 
CCTGGCCTGG GCTCTGAGAA CATGGATCGA GGAAATAACA ATGTAATGAG CAATTATGAG 
GCCTACAAGC CTTCCACAGG AGCTATGGGA GATCGACTAA CGGCAATGAA AGCAGCTTTC 
CAGTCACAGT ACAAGAGTCA CTTTGTTGCA GCCAGTTTAA GTAATCAGAA GGCTGGAAGT 
TCTGCTGCTG GGGCAAGTGG GTGGACTAGT GCAGGGAGCT TGAATTCTGT TCCAACTAAC 
TCAGCACAAC AGGGCCATAA CAGTCCTGAC AGCCCCGTCA CCAGTGCCGC CAAGGGCATC 
CCAGGCTTTG GCAATACTGG CAACATCAGT GGTGCCCCTG TGACCTACCC GTCTGCCGGA 
GCCCAAGGAG TCAACAACAC AGCTTCAGGG AATAACAGCC GAGAAGGGAC TGGGGGCAGC 
AACGGGAAAA GAGAGAGATA TACTGAGAAC CGGGGCAGCA GCCGTCACAG TCACGGAGAG 
ACTGGCAATC GGCATAGCGA TAGTCCACGT CACGGAGATG GTGGTCGCCA TGGAGATGGA 
TACCGCCATC CAGAAAGCAG CAGCCGTCAT ACTGATGGCC ATCGGCACGG GGAGAACAGA 
CATGGAGGAA GCGCAGGCCG GCATGGGGAG AACCGGGGTG CAAATGATGG TCGGAATGGG 
GAAAGCAGGA AAGAAGCTTT TAATCGTGAG AGCAAGATGG AGCCCAAGAT GGAACCCAAA 
GTGGACAGCA GCAAGATGGA CAAGGTGGAC AGCAAGACAG ATAAGACAGC TGACGGCTTT 
GCTGTCCCAG AGCCGCCTAA ACGCAAGAAA AGTCGATGGG ACAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX42 ( XM_017024112.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879601.1 Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu86545
Clone ID Related Accession (Same CDS sequence)	XM_024450558.1 , XM_017024112.1
Accession Version	XM_017024112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX42 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA ACCCAACTGC TGGTGTGGTT CAGGAGGAAG AGGAAGACAA TCTAGAATAT 
GATAGTGACG GAAATCCAAT TGCACCTACC AAAAAAATCA TTGATCCTCT TCCCCCCATT 
GATCATTCAG AGATTGACTA TCCACCATTT GAAAAAAACT TTTACAATGA GCATGAAGAG 
ATAACCAACC TCACTCCACA GCAGTTAATA GATCTCCGGC ATAAGCTCAA TCTTCGGGTC 
TCTGGTGCTG CACCTCCTAG ACCAGGAAGT AGCTTTGCTC ATTTTGGGTT TGACGAACAA 
CTTATGCACC AGATTCGGAA ATCTGAATAC ACACAGCCCA CTCCAATACA GTGCCAGGGT 
GTGCCTGTGG CATTAAGTGG TAGAGACATG ATTGGTATTG CCAAAACAGG TAGTGGGAAA 
ACTGCAGCCT TCATTTGGCC CATGTTGATT CATATAATGG ACCAGAAGGA GTTGGAACCA 
GGTGATGGAC CAATTGCAGT GATTGTGTGT CCTACCAGGG AGCTTTGCCA GCAGATCCAT 
GCAGAATGTA AGCGGTTTGG AAAAGCATAT AATCTTCGAT CAGTGGCCGT ATATGGAGGA 
GGGAGTATGT GGGAGCAGGC CAAGGCCCTT CAGGAGGGGG CAGAGATTGT TGTGTGTACC 
CCAGGTCGAC TGATAGATCA TGTGAAAAAG AAAGCTACCA ATCTTCAAAG AGTCTCTTAC 
CTTGTGTTTG ATGAAGCAGA TCGAATGTTT GACATGGGAT TTGAGTACCA AGTTCGATCC 
ATAGCAAGTC ATGTTCGTCC TGACAGGCAG ACTCTCTTAT TTAGTGCAAC TTTTCGGAAG 
AAGATTGAAA AGTTGGCCAG AGACATCCTG ATCGACCCTA TTCGAGTGGT GCAGGGAGAT 
ATTGGAGAGG CAAATGAAGA TGTGACACAG ATTGTGGAGA TTCTCCATTC TGGACCTAGT 
AAATGGAACT GGCTTACCCG GCGTCTGGTA GAATTTACCT CTTCAGGGAG TGTCCTCCTC 
TTTGTTACTA AAAAAGCCAA TGCTGAAGAG CTAGCGAATA ACCTTAAACA GGAGGGTCAT 
AATCTTGGGC TGCTCCATGG GGATATGGAT CAGAGTGAGA GAAACAAGGT CATTTCAGAC 
TTTAAGAAAA AGGACATCCC AGTCCTGGTG GCCACAGATG TTGCAGCCCG TGGTCTGGAC 
ATTCCTTCAA TTAAGACTGT CATTAACTAT GATGTGGCAC GAGACATTGA TACCCACACG 
CATAGGATTG GCCGCACAGG AAGAGCGGGT GAGAAAGGTG TGGCCTATAC CCTACTCACT 
CCCAAGGACA GCAATTTTGC TGGTGACCTG GTCCGGAACT TGGAAGGAGC CAATCAACAC 
GTTTCTAAGG AACTCCTAGA TCTGGCAATG CAGAATGCCT GGTTTCGGAA ATCTCGATTC 
AAAGGAGGGA AAGGAAAAAA GCTGAACATT GGTGGAGGAG GCCTAGGCTA CAGGGAGCGG 
CCTGGCCTGG GCTCTGAGAA CATGGATCGA GGAAATAACA ATGTAATGAG CAATTATGAG 
GCCTACAAGC CTTCCACAGG AGCTATGGGA GATCGACTAA CGGCAATGAA AGCAGCTTTC 
CAGTCACAGT ACAAGAGTCA CTTTGTTGCA GCCAGTTTAA GTAATCAGAA GGCTGGAAGT 
TCTGCTGCTG GGGCAAGTGG GTGGACTAGT GCAGGGAGCT TGAATTCTGT TCCAACTAAC 
TCAGCACAAC AGGGCCATAA CAGTCCTGAC AGCCCCGTCA CCAGTGCCGC CAAGGGCATC 
CCAGGCTTTG GCAATACTGG CAACATCAGT GGTGCCCCTG TGACCTACCC GTCTGCCGGA 
GCCCAAGGAG TCAACAACAC AGCTTCAGGG AATAACAGCC GAGAAGGGAC TGGGGGCAGC 
AACGGGAAAA GAGAGAGATA TACTGAGAAC CGGGGCAGCA GCCGTCACAG TCACGGAGAG 
ACTGGCAATC GGCATAGCGA TAGTCCACGT CACGGAGATG GTGGTCGCCA TGGAGATGGA 
TACCGCCATC CAGAAAGCAG CAGCCGTCAT ACTGATGGCC ATCGGCACGG GGAGAACAGA 
CATGGAGGAA GCGCAGGCCG GCATGGGGAG AACCGGGGTG CAAATGATGG TCGGAATGGG 
GAAAGCAGGA AAGAAGCTTT TAATCGTGAG AGCAAGATGG AGCCCAAGAT GGAACCCAAA 
GTGGACAGCA GCAAGATGGA CAAGGTGGAC AGCAAGACAG ATAAGACAGC TGACGGCTTT 
GCTGTCCCAG AGCCGCCTAA ACGCAAGAAA AGTCGATGGG ACAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879601.1
CDS	81..2408
Translation	MAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Target ORF information:

RefSeq Version	XM_017024112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 42 (DDX42), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024112.1

ORF Insert Sequence:

ATGGCAGAAA ACCCAACTGC TGGTGTGGTT CAGGAGGAAG AGGAAGACAA TCTAGAATAT 
GATAGTGACG GAAATCCAAT TGCACCTACC AAAAAAATCA TTGATCCTCT TCCCCCCATT 
GATCATTCAG AGATTGACTA TCCACCATTT GAAAAAAACT TTTACAATGA GCATGAAGAG 
ATAACCAACC TCACTCCACA GCAGTTAATA GATCTCCGGC ATAAGCTCAA TCTTCGGGTC 
TCTGGTGCTG CACCTCCTAG ACCAGGAAGT AGCTTTGCTC ATTTTGGGTT TGACGAACAA 
CTTATGCACC AGATTCGGAA ATCTGAATAC ACACAGCCCA CTCCAATACA GTGCCAGGGT 
GTGCCTGTGG CATTAAGTGG TAGAGACATG ATTGGTATTG CCAAAACAGG TAGTGGGAAA 
ACTGCAGCCT TCATTTGGCC CATGTTGATT CATATAATGG ACCAGAAGGA GTTGGAACCA 
GGTGATGGAC CAATTGCAGT GATTGTGTGT CCTACCAGGG AGCTTTGCCA GCAGATCCAT 
GCAGAATGTA AGCGGTTTGG AAAAGCATAT AATCTTCGAT CAGTGGCCGT ATATGGAGGA 
GGGAGTATGT GGGAGCAGGC CAAGGCCCTT CAGGAGGGGG CAGAGATTGT TGTGTGTACC 
CCAGGTCGAC TGATAGATCA TGTGAAAAAG AAAGCTACCA ATCTTCAAAG AGTCTCTTAC 
CTTGTGTTTG ATGAAGCAGA TCGAATGTTT GACATGGGAT TTGAGTACCA AGTTCGATCC 
ATAGCAAGTC ATGTTCGTCC TGACAGGCAG ACTCTCTTAT TTAGTGCAAC TTTTCGGAAG 
AAGATTGAAA AGTTGGCCAG AGACATCCTG ATCGACCCTA TTCGAGTGGT GCAGGGAGAT 
ATTGGAGAGG CAAATGAAGA TGTGACACAG ATTGTGGAGA TTCTCCATTC TGGACCTAGT 
AAATGGAACT GGCTTACCCG GCGTCTGGTA GAATTTACCT CTTCAGGGAG TGTCCTCCTC 
TTTGTTACTA AAAAAGCCAA TGCTGAAGAG CTAGCGAATA ACCTTAAACA GGAGGGTCAT 
AATCTTGGGC TGCTCCATGG GGATATGGAT CAGAGTGAGA GAAACAAGGT CATTTCAGAC 
TTTAAGAAAA AGGACATCCC AGTCCTGGTG GCCACAGATG TTGCAGCCCG TGGTCTGGAC 
ATTCCTTCAA TTAAGACTGT CATTAACTAT GATGTGGCAC GAGACATTGA TACCCACACG 
CATAGGATTG GCCGCACAGG AAGAGCGGGT GAGAAAGGTG TGGCCTATAC CCTACTCACT 
CCCAAGGACA GCAATTTTGC TGGTGACCTG GTCCGGAACT TGGAAGGAGC CAATCAACAC 
GTTTCTAAGG AACTCCTAGA TCTGGCAATG CAGAATGCCT GGTTTCGGAA ATCTCGATTC 
AAAGGAGGGA AAGGAAAAAA GCTGAACATT GGTGGAGGAG GCCTAGGCTA CAGGGAGCGG 
CCTGGCCTGG GCTCTGAGAA CATGGATCGA GGAAATAACA ATGTAATGAG CAATTATGAG 
GCCTACAAGC CTTCCACAGG AGCTATGGGA GATCGACTAA CGGCAATGAA AGCAGCTTTC 
CAGTCACAGT ACAAGAGTCA CTTTGTTGCA GCCAGTTTAA GTAATCAGAA GGCTGGAAGT 
TCTGCTGCTG GGGCAAGTGG GTGGACTAGT GCAGGGAGCT TGAATTCTGT TCCAACTAAC 
TCAGCACAAC AGGGCCATAA CAGTCCTGAC AGCCCCGTCA CCAGTGCCGC CAAGGGCATC 
CCAGGCTTTG GCAATACTGG CAACATCAGT GGTGCCCCTG TGACCTACCC GTCTGCCGGA 
GCCCAAGGAG TCAACAACAC AGCTTCAGGG AATAACAGCC GAGAAGGGAC TGGGGGCAGC 
AACGGGAAAA GAGAGAGATA TACTGAGAAC CGGGGCAGCA GCCGTCACAG TCACGGAGAG 
ACTGGCAATC GGCATAGCGA TAGTCCACGT CACGGAGATG GTGGTCGCCA TGGAGATGGA 
TACCGCCATC CAGAAAGCAG CAGCCGTCAT ACTGATGGCC ATCGGCACGG GGAGAACAGA 
CATGGAGGAA GCGCAGGCCG GCATGGGGAG AACCGGGGTG CAAATGATGG TCGGAATGGG 
GAAAGCAGGA AAGAAGCTTT TAATCGTGAG AGCAAGATGG AGCCCAAGAT GGAACCCAAA 
GTGGACAGCA GCAAGATGGA CAAGGTGGAC AGCAAGACAG ATAAGACAGC TGACGGCTTT 
GCTGTCCCAG AGCCGCCTAA ACGCAAGAAA AGTCGATGGG ACAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

The DEAD box protein Ddx42p modulates the function of ASPP2, a stimulator of apoptosis.
Oncogene28(20)2065-73(2009 May)
Uhlmann-Schiffler H,Kiermayer S,Stahl H

Interferon antagonist function of Japanese encephalitis virus NS4A and its interaction with DEAD-box RNA helicase DDX42.
Virus research137(1)49-55(2008 Oct)
Lin CW,Cheng CW,Yang TC,Li SW,Cheng MH,Wan L,Lin YJ,Lai CH,Lin WY,Kao MC

Ddx42p--a human DEAD box protein with RNA chaperone activities.
Nucleic acids research34(1)10-22(2006)
Uhlmann-Schiffler H,Jalal C,Stahl H

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DDX42 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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