RAPGEF4 cDNA ORF clone, Homo sapiens(human)

The following RAPGEF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu109022 NM_001375875.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu109025 NM_001375876.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu20982 XM_006712205.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu35775 XM_005246246.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu64677 XM_011510505.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu109023 NM_001375868.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109024 NM_001375872.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109027 NM_001375870.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109028 NM_001375874.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109029 NM_001375869.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109030 NM_001375871.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109032 NM_001375867.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109033 NM_001375873.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu17215 NM_001100397.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu20982 NM_001282900.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu21563 NM_001282901.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu21628 NM_001282899.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu35775 NM_001375866.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu88944 XM_017003196.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu88945 XM_017003197.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu109026 NM_001375864.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu109031 NM_001375865.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu17245 NM_007023.4
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
View Old Accession Versions >>
OHu35775 XM_005246246.2 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu20982 NM_001282900.1 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu17245 NM_007023.3 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu17215 NM_001100397.1 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu21563 NM_001282901.1 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu88944 XM_017003196.1 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu64677 XM_011510505.2 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu21628 NM_001282899.1 Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu109022
    Clone ID Related Accession (Same CDS sequence) NM_001375875.1
    Accession Version NM_001375875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform q
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3 and AC104086.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.285551.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCGGTGTG GAATTTAAAA
    TCCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362804.1
    CDS140..1168
    Translation

    Target ORF information:

    RefSeq Version NM_001375875.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCGGTGTG GAATTTAAAA
    TCCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109025
    Clone ID Related Accession (Same CDS sequence) NM_001375876.1
    Accession Version NM_001375876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform r
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3 and AC104086.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.24796.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC GGTGTGGAAT
    TTAAAATCCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362805.1
    CDS140..1114
    Translation

    Target ORF information:

    RefSeq Version NM_001375876.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC GGTGTGGAAT
    TTAAAATCCT GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17215
    Clone ID Related Accession (Same CDS sequence) NM_001100397.1 , NM_001100397.2
    Accession Version NM_001100397.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC040183.2, BC024004.2, AB027471.1 and BQ184791.1. Transcript Variant: This variant (2) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040183.2, SRR1660807.143230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCTACA AGAAATATCG ACAGTATATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TCCCTTCAGA GAAGATCCTC
    AGAGCTGGAA AAATTTTACG AAATGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAA TGCTGTGTGG GAACTGAACT GGTGGACTGG
    ATGATGCAGC AGACACCATG TGTTCACTCC CGGACTCAAG CTGTTGGCAT GTGGCAAGTC
    CTGTTAGAAG ATGGTGTTCT CAACCACGTG GACCAGGAGC ACCATTTCCA AGACAAATAT
    TTATTCTATC GATTTCTGGA TGATGAGCAC GAGGATGCCC CTTTGCCTAC TGAGGAGGAG
    AAGAAGGAGT GTGATGAGGA GCTCCAGGAC ACCATGCTGC TGCTGTCACA GATGGGCCCC
    GACGCCCACA TGAGGATGAT CCTTCGCAAA CCACCTGGCC AGAGGACTGT GGATGACCTA
    GAGATTATCT ATGAGGAGCT TCTTCATATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGAGAGT TAGCAGGTGT TCTCATTTTT GAGTCTCACG CCAAAGGAGG GACTGTGTTG
    TTTAACCAGG GGGAAGAAGG TACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTACG GCAAGGGTGT GGTCTGCACC CTGCATGAAG GAGATGACTT CGGCAAGTTA
    GCACTAGTGA ATGATGCCCC ACGAGCTGCC TCTATCGTCT TACGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTAA GGGACGTGGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGAGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACAATACGCC TTGAGGCAAC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTATT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CGGCACTGGT GGCCCACTAC CACGCACAGC CTTCACAAGG TACAGAACAG
    GAGAAAATGG ATTATGCCCT CAACAATAAG AGGCGAGTCA TCCGCCTGGT TCTACAGTGG
    GCTGCCATGT ATGGAGACCT CCTGCAAGAG GATGACGTGT CTATGGCCTT CCTGGAGGAG
    TTTTATGTAT CTGTATCAGA TGATGCCCGG ATGATTGCTG CCCTCAAGGA GCAACTGCCA
    GAGTTGGAGA AGATTGTCAA GCAAATCTCA GAAGATGCAA AGGCACCACA AAAGAAGCAC
    AAGGTTCTTT TGCAACAGTT CAATACGGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGCGGCTCTG ATGAAGTTCT GTTTAAGGTC TATTGCATGG ACCACACCTA CACAACCATT
    CGGGTGCCAG TGGCCACTTC GGTGAAGGAA GTCATCAGTG CAGTTGCCGA CAAGCTGGGC
    TCCGGGGAGG GCCTGATCAT AGTCAAGATG AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACGACGCTC ACCATTAATG GACGCCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTA CCAGAACAGG AAGGCCCAAC TGTTGGAACA
    GTGGGAACTT TTGAACTGAT GAGCTCCAAA GATTTAGCAT ACCAGATGAC AATTTATGAT
    TGGGAACTCT TCAACTGCGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    AATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGATTTAA TGAAATTCAG
    TTTTGGGTCG TCACTGAGAT CTGCCTTTGT TCTCAGCTCA GCAAGCGTGT TCAGCTATTA
    AAAAAATTTA TTAAGATAGC AGCCCACTGT AAGGAGTATA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAACGTTGCT GTGAGCCGCT TGGCACTAAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCG GAGTTTGAAA GTTTAATGGA CCCTTCAAGG
    AACCACAGGG CCTACAGGCT GACAGTAGCT AAGCTGGAAC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTAGTAAACT TTGAAAAAAT GCGCATGATT GCAAATACGG CCAGAACAGT GAGATACTAC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCATCA GGATGTCCGG
    AGTTATGTAC GGCAATTAAA TGTGATTGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093867.1
    CDS379..2982
    Translation

    Target ORF information:

    RefSeq Version NM_001100397.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCTACA AGAAATATCG ACAGTATATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TCCCTTCAGA GAAGATCCTC
    AGAGCTGGAA AAATTTTACG AAATGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAA TGCTGTGTGG GAACTGAACT GGTGGACTGG
    ATGATGCAGC AGACACCATG TGTTCACTCC CGGACTCAAG CTGTTGGCAT GTGGCAAGTC
    CTGTTAGAAG ATGGTGTTCT CAACCACGTG GACCAGGAGC ACCATTTCCA AGACAAATAT
    TTATTCTATC GATTTCTGGA TGATGAGCAC GAGGATGCCC CTTTGCCTAC TGAGGAGGAG
    AAGAAGGAGT GTGATGAGGA GCTCCAGGAC ACCATGCTGC TGCTGTCACA GATGGGCCCC
    GACGCCCACA TGAGGATGAT CCTTCGCAAA CCACCTGGCC AGAGGACTGT GGATGACCTA
    GAGATTATCT ATGAGGAGCT TCTTCATATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGAGAGT TAGCAGGTGT TCTCATTTTT GAGTCTCACG CCAAAGGAGG GACTGTGTTG
    TTTAACCAGG GGGAAGAAGG TACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTACG GCAAGGGTGT GGTCTGCACC CTGCATGAAG GAGATGACTT CGGCAAGTTA
    GCACTAGTGA ATGATGCCCC ACGAGCTGCC TCTATCGTCT TACGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTAA GGGACGTGGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGAGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACAATACGCC TTGAGGCAAC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTATT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CGGCACTGGT GGCCCACTAC CACGCACAGC CTTCACAAGG TACAGAACAG
    GAGAAAATGG ATTATGCCCT CAACAATAAG AGGCGAGTCA TCCGCCTGGT TCTACAGTGG
    GCTGCCATGT ATGGAGACCT CCTGCAAGAG GATGACGTGT CTATGGCCTT CCTGGAGGAG
    TTTTATGTAT CTGTATCAGA TGATGCCCGG ATGATTGCTG CCCTCAAGGA GCAACTGCCA
    GAGTTGGAGA AGATTGTCAA GCAAATCTCA GAAGATGCAA AGGCACCACA AAAGAAGCAC
    AAGGTTCTTT TGCAACAGTT CAATACGGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGCGGCTCTG ATGAAGTTCT GTTTAAGGTC TATTGCATGG ACCACACCTA CACAACCATT
    CGGGTGCCAG TGGCCACTTC GGTGAAGGAA GTCATCAGTG CAGTTGCCGA CAAGCTGGGC
    TCCGGGGAGG GCCTGATCAT AGTCAAGATG AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACGACGCTC ACCATTAATG GACGCCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTA CCAGAACAGG AAGGCCCAAC TGTTGGAACA
    GTGGGAACTT TTGAACTGAT GAGCTCCAAA GATTTAGCAT ACCAGATGAC AATTTATGAT
    TGGGAACTCT TCAACTGCGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    AATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGATTTAA TGAAATTCAG
    TTTTGGGTCG TCACTGAGAT CTGCCTTTGT TCTCAGCTCA GCAAGCGTGT TCAGCTATTA
    AAAAAATTTA TTAAGATAGC AGCCCACTGT AAGGAGTATA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAACGTTGCT GTGAGCCGCT TGGCACTAAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCG GAGTTTGAAA GTTTAATGGA CCCTTCAAGG
    AACCACAGGG CCTACAGGCT GACAGTAGCT AAGCTGGAAC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTAGTAAACT TTGAAAAAAT GCGCATGATT GCAAATACGG CCAGAACAGT GAGATACTAC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCATCA GGATGTCCGG
    AGTTATGTAC GGCAATTAAA TGTGATTGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20982
    Clone ID Related Accession (Same CDS sequence) NM_001282900.2 , NM_001282900.1 , XM_006712205.3
    Accession Version NM_001282900.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform d
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC335305.1, AK296340.1, U78516.1 and BQ184791.1. On Sep 20, 2013 this sequence version replaced XM_005246249.1. Transcript Variant: This variant (4) differs in its 5' UTR, lacks several exons in the 5' coding region, initiates translation at a downstream in-frame start codon, and lacks an additional in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.207914.1, SRR1803617.114623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269829.1
    CDS277..2799
    Translation

    Target ORF information:

    RefSeq Version NM_001282900.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21563
    Clone ID Related Accession (Same CDS sequence) NM_001282901.1 , NM_001282901.2
    Accession Version NM_001282901.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform e
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK311575.1, AK294433.1, U78516.1 and BQ184791.1. Transcript Variant: This variant (5) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (e) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA 
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269830.1
    CDS212..2587
    Translation

    Target ORF information:

    RefSeq Version NM_001282901.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA 
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21628
    Clone ID Related Accession (Same CDS sequence) NM_001282899.1 , NM_001282899.2
    Accession Version NM_001282899.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC335305.1, AK316072.1, U78516.1 and BQ184791.1. On Sep 20, 2013 this sequence version replaced XM_005246247.1. Transcript Variant: This variant (3) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.8134.1, SRR1803614.117433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGAGCTG GAAAAATTTT ACGAAATGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAATGCTGTG TGGGAACTGA ACTGGTGGAC TGGATGATGC AGCAGACACC ATGTGTTCAC
    TCCCGGACTC AAGCTGTTGG CATGTGGCAA GTCCTGTTAG AAGATGGTGT TCTCAACCAC
    GTGGACCAGG AGCACCATTT CCAAGACAAA TATTTATTCT ATCGATTTCT GGATGATGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGACGCCC ACATGAGGAT GATCCTTCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAGATTA TCTATGAGGA GCTTCTTCAT
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGAG AGTTAGCAGG TGTTCTCATT
    TTTGAGTCTC ACGCCAAAGG AGGGACTGTG TTGTTTAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ACGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AAGGAGATGA CTTCGGCAAG TTAGCACTAG TGAATGATGC CCCACGAGCT
    GCCTCTATCG TCTTACGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TAAGGGACGT GGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGAGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGGC AACTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    ATTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCGGCACT GGTGGCCCAC
    TACCACGCAC AGCCTTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGGCGAG TCATCCGCCT GGTTCTACAG TGGGCTGCCA TGTATGGAGA CCTCCTGCAA
    GAGGATGACG TGTCTATGGC CTTCCTGGAG GAGTTTTATG TATCTGTATC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCAGAGTTGG AGAAGATTGT CAAGCAAATC
    TCAGAAGATG CAAAGGCACC ACAAAAGAAG CACAAGGTTC TTTTGCAACA GTTCAATACG
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGCGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CAGTGGCCAC TTCGGTGAAG
    GAAGTCATCA GTGCAGTTGC CGACAAGCTG GGCTCCGGGG AGGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACGACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTACCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG CGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGATT TAATGAAATT CAGTTTTGGG TCGTCACTGA GATCTGCCTT
    TGTTCTCAGC TCAGCAAGCG TGTTCAGCTA TTAAAAAAAT TTATTAAGAT AGCAGCCCAC
    TGTAAGGAGT ATAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAACGTT
    GCTGTGAGCC GCTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGTTTAAT GGACCCTTCA AGGAACCACA GGGCCTACAG GCTGACAGTA
    GCTAAGCTGG AACCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CGGCCAGAAC AGTGAGATAC TACAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269828.1
    CDS277..2853
    Translation

    Target ORF information:

    RefSeq Version NM_001282899.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGAGCTG GAAAAATTTT ACGAAATGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAATGCTGTG TGGGAACTGA ACTGGTGGAC TGGATGATGC AGCAGACACC ATGTGTTCAC
    TCCCGGACTC AAGCTGTTGG CATGTGGCAA GTCCTGTTAG AAGATGGTGT TCTCAACCAC
    GTGGACCAGG AGCACCATTT CCAAGACAAA TATTTATTCT ATCGATTTCT GGATGATGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGACGCCC ACATGAGGAT GATCCTTCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAGATTA TCTATGAGGA GCTTCTTCAT
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGAG AGTTAGCAGG TGTTCTCATT
    TTTGAGTCTC ACGCCAAAGG AGGGACTGTG TTGTTTAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ACGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AAGGAGATGA CTTCGGCAAG TTAGCACTAG TGAATGATGC CCCACGAGCT
    GCCTCTATCG TCTTACGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TAAGGGACGT GGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGAGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGGC AACTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    ATTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCGGCACT GGTGGCCCAC
    TACCACGCAC AGCCTTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGGCGAG TCATCCGCCT GGTTCTACAG TGGGCTGCCA TGTATGGAGA CCTCCTGCAA
    GAGGATGACG TGTCTATGGC CTTCCTGGAG GAGTTTTATG TATCTGTATC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCAGAGTTGG AGAAGATTGT CAAGCAAATC
    TCAGAAGATG CAAAGGCACC ACAAAAGAAG CACAAGGTTC TTTTGCAACA GTTCAATACG
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGCGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CAGTGGCCAC TTCGGTGAAG
    GAAGTCATCA GTGCAGTTGC CGACAAGCTG GGCTCCGGGG AGGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACGACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTACCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG CGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGATT TAATGAAATT CAGTTTTGGG TCGTCACTGA GATCTGCCTT
    TGTTCTCAGC TCAGCAAGCG TGTTCAGCTA TTAAAAAAAT TTATTAAGAT AGCAGCCCAC
    TGTAAGGAGT ATAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAACGTT
    GCTGTGAGCC GCTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGTTTAAT GGACCCTTCA AGGAACCACA GGGCCTACAG GCTGACAGTA
    GCTAAGCTGG AACCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CGGCCAGAAC AGTGAGATAC TACAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20982
    Clone ID Related Accession (Same CDS sequence) NM_001282900.2 , NM_001282900.1 , XM_006712205.3
    Accession Version XM_006712205.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712268.1
    CDS519..3041
    Translation

    Target ORF information:

    RefSeq Version XM_006712205.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712205.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35775
    Clone ID Related Accession (Same CDS sequence) XM_005246246.3 , XM_005246246.2 , NM_001375866.1
    Accession Version XM_005246246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005246246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACGGCC
    AGAACAGTGA GATACTACAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246303.1
    CDS66..3047
    Translation

    Target ORF information:

    RefSeq Version XM_005246246.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACGGCC
    AGAACAGTGA GATACTACAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35775
    Clone ID Related Accession (Same CDS sequence) XM_005246246.3 , XM_005246246.2 , NM_001375866.1
    Accession Version XM_005246246.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACGGCC
    AGAACAGTGA GATACTACAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005246303.1
    CDS55..3036
    Translation

    Target ORF information:

    RefSeq Version XM_005246246.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005246246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACGGCC
    AGAACAGTGA GATACTACAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64677
    Clone ID Related Accession (Same CDS sequence) XM_011510505.2 , XM_011510505.3
    Accession Version XM_011510505.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG AAGGACTTCA 
    AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT CCTTATGGTG
    TTATGGAAAC GGGCTCTAAC AATGACAACA CCTCTCATTG AACCTCACGT TCCTCTTCGT
    CCTGCTAACA CCATTACCAA GGTCCCTTCA GAGAAGATCC TCAGAGCTGG AAAAATTTTA
    CGAAATGCCA TTCTCTCTCG AGCACCTCAC ATGATAAGAG ATAGAAAATA CCACCTAAAG
    ACATACAGAC AATGCTGTGT GGGAACTGAA CTGGTGGACT GGATGATGCA GCAGACACCA
    TGTGTTCACT CCCGGACTCA AGCTGTTGGC ATGTGGCAAG TCCTGTTAGA AGATGGTGTT
    CTCAACCACG TGGACCAGGA GCACCATTTC CAAGACAAAT ATTTATTCTA TCGATTTCTG
    GATGATGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGACGCCCA CATGAGGATG
    ATCCTTCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAGATTAT CTATGAGGAG
    CTTCTTCATA TTAAAGCCTT ATCCCATCTT TCTACCACAG TGAAACGAGA GTTAGCAGGT
    GTTCTCATTT TTGAGTCTCA CGCCAAAGGA GGGACTGTGT TGTTTAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCAGTGAATG TAGTCATTTA CGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA AGGAGATGAC TTCGGCAAGT TAGCACTAGT GAATGATGCC
    CCACGAGCTG CCTCTATCGT CTTACGAGAA GATAACTGCC ATTTCTTAAG AGTAGACAAG
    GAGGATTTCA ACCGGATCCT AAGGGACGTG GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGAGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGGCA ACTTTAAATG AAGCAACAGA TTCTGTTTTA
    AATGACTTTA TTATGATGCA CTGTGTTTTT ATGCCAAATA CCCAGCTTTG CCCGGCACTG
    GTGGCCCACT ACCACGCACA GCCTTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGGCGAGT CATCCGCCTG GTTCTACAGT GGGCTGCCAT GTATGGAGAC
    CTCCTGCAAG AGGATGACGT GTCTATGGCC TTCCTGGAGG AGTTTTATGT ATCTGTATCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CAGAGTTGGA GAAGATTGTC
    AAGCAAATCT CAGAAGATGC AAAGGCACCA CAAAAGAAGC ACAAGGTTCT TTTGCAACAG
    TTCAATACGG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGCGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC AGTGGCCACT
    TCGGTGAAGG AAGTCATCAG TGCAGTTGCC GACAAGCTGG GCTCCGGGGA GGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACGACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGATTCA
    CTGACTCCCT TACCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGC
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGGAGATTT AATGAAATTC AGTTTTGGGT CGTCACTGAG
    ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT GTTCAGCTAT TAAAAAAATT TATTAAGATA
    GCAGCCCACT GTAAGGAGTA TAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAACGTTG CTGTGAGCCG CTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGTTTAATG GACCCTTCAA GGAACCACAG GGCCTACAGG
    CTGACAGTAG CTAAGCTGGA ACCTCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACG TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC GGCCAGAACA GTGAGATACT ACAGGAGCCA ACCCTTCAAT
    CCTGATGCAG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508807.1
    CDS425..3070
    Translation

    Target ORF information:

    RefSeq Version XM_011510505.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG AAGGACTTCA 
    AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT CCTTATGGTG
    TTATGGAAAC GGGCTCTAAC AATGACAACA CCTCTCATTG AACCTCACGT TCCTCTTCGT
    CCTGCTAACA CCATTACCAA GGTCCCTTCA GAGAAGATCC TCAGAGCTGG AAAAATTTTA
    CGAAATGCCA TTCTCTCTCG AGCACCTCAC ATGATAAGAG ATAGAAAATA CCACCTAAAG
    ACATACAGAC AATGCTGTGT GGGAACTGAA CTGGTGGACT GGATGATGCA GCAGACACCA
    TGTGTTCACT CCCGGACTCA AGCTGTTGGC ATGTGGCAAG TCCTGTTAGA AGATGGTGTT
    CTCAACCACG TGGACCAGGA GCACCATTTC CAAGACAAAT ATTTATTCTA TCGATTTCTG
    GATGATGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGACGCCCA CATGAGGATG
    ATCCTTCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAGATTAT CTATGAGGAG
    CTTCTTCATA TTAAAGCCTT ATCCCATCTT TCTACCACAG TGAAACGAGA GTTAGCAGGT
    GTTCTCATTT TTGAGTCTCA CGCCAAAGGA GGGACTGTGT TGTTTAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCAGTGAATG TAGTCATTTA CGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA AGGAGATGAC TTCGGCAAGT TAGCACTAGT GAATGATGCC
    CCACGAGCTG CCTCTATCGT CTTACGAGAA GATAACTGCC ATTTCTTAAG AGTAGACAAG
    GAGGATTTCA ACCGGATCCT AAGGGACGTG GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGAGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGGCA ACTTTAAATG AAGCAACAGA TTCTGTTTTA
    AATGACTTTA TTATGATGCA CTGTGTTTTT ATGCCAAATA CCCAGCTTTG CCCGGCACTG
    GTGGCCCACT ACCACGCACA GCCTTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGGCGAGT CATCCGCCTG GTTCTACAGT GGGCTGCCAT GTATGGAGAC
    CTCCTGCAAG AGGATGACGT GTCTATGGCC TTCCTGGAGG AGTTTTATGT ATCTGTATCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CAGAGTTGGA GAAGATTGTC
    AAGCAAATCT CAGAAGATGC AAAGGCACCA CAAAAGAAGC ACAAGGTTCT TTTGCAACAG
    TTCAATACGG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGCGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC AGTGGCCACT
    TCGGTGAAGG AAGTCATCAG TGCAGTTGCC GACAAGCTGG GCTCCGGGGA GGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACGACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGATTCA
    CTGACTCCCT TACCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGC
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGGAGATTT AATGAAATTC AGTTTTGGGT CGTCACTGAG
    ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT GTTCAGCTAT TAAAAAAATT TATTAAGATA
    GCAGCCCACT GTAAGGAGTA TAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAACGTTG CTGTGAGCCG CTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGTTTAATG GACCCTTCAA GGAACCACAG GGCCTACAGG
    CTGACAGTAG CTAAGCTGGA ACCTCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACG TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC GGCCAGAACA GTGAGATACT ACAGGAGCCA ACCCTTCAAT
    CCTGATGCAG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64677
    Clone ID Related Accession (Same CDS sequence) XM_011510505.2 , XM_011510505.3
    Accession Version XM_011510505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG AAGGACTTCA 
    AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT CCTTATGGTG
    TTATGGAAAC GGGCTCTAAC AATGACAACA CCTCTCATTG AACCTCACGT TCCTCTTCGT
    CCTGCTAACA CCATTACCAA GGTCCCTTCA GAGAAGATCC TCAGAGCTGG AAAAATTTTA
    CGAAATGCCA TTCTCTCTCG AGCACCTCAC ATGATAAGAG ATAGAAAATA CCACCTAAAG
    ACATACAGAC AATGCTGTGT GGGAACTGAA CTGGTGGACT GGATGATGCA GCAGACACCA
    TGTGTTCACT CCCGGACTCA AGCTGTTGGC ATGTGGCAAG TCCTGTTAGA AGATGGTGTT
    CTCAACCACG TGGACCAGGA GCACCATTTC CAAGACAAAT ATTTATTCTA TCGATTTCTG
    GATGATGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGACGCCCA CATGAGGATG
    ATCCTTCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAGATTAT CTATGAGGAG
    CTTCTTCATA TTAAAGCCTT ATCCCATCTT TCTACCACAG TGAAACGAGA GTTAGCAGGT
    GTTCTCATTT TTGAGTCTCA CGCCAAAGGA GGGACTGTGT TGTTTAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCAGTGAATG TAGTCATTTA CGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA AGGAGATGAC TTCGGCAAGT TAGCACTAGT GAATGATGCC
    CCACGAGCTG CCTCTATCGT CTTACGAGAA GATAACTGCC ATTTCTTAAG AGTAGACAAG
    GAGGATTTCA ACCGGATCCT AAGGGACGTG GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGAGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGGCA ACTTTAAATG AAGCAACAGA TTCTGTTTTA
    AATGACTTTA TTATGATGCA CTGTGTTTTT ATGCCAAATA CCCAGCTTTG CCCGGCACTG
    GTGGCCCACT ACCACGCACA GCCTTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGGCGAGT CATCCGCCTG GTTCTACAGT GGGCTGCCAT GTATGGAGAC
    CTCCTGCAAG AGGATGACGT GTCTATGGCC TTCCTGGAGG AGTTTTATGT ATCTGTATCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CAGAGTTGGA GAAGATTGTC
    AAGCAAATCT CAGAAGATGC AAAGGCACCA CAAAAGAAGC ACAAGGTTCT TTTGCAACAG
    TTCAATACGG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGCGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC AGTGGCCACT
    TCGGTGAAGG AAGTCATCAG TGCAGTTGCC GACAAGCTGG GCTCCGGGGA GGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACGACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGATTCA
    CTGACTCCCT TACCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGC
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGGAGATTT AATGAAATTC AGTTTTGGGT CGTCACTGAG
    ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT GTTCAGCTAT TAAAAAAATT TATTAAGATA
    GCAGCCCACT GTAAGGAGTA TAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAACGTTG CTGTGAGCCG CTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGTTTAATG GACCCTTCAA GGAACCACAG GGCCTACAGG
    CTGACAGTAG CTAAGCTGGA ACCTCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACG TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC GGCCAGAACA GTGAGATACT ACAGGAGCCA ACCCTTCAAT
    CCTGATGCAG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508807.1
    CDS436..3081
    Translation

    Target ORF information:

    RefSeq Version XM_011510505.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG AAGGACTTCA 
    AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT CCTTATGGTG
    TTATGGAAAC GGGCTCTAAC AATGACAACA CCTCTCATTG AACCTCACGT TCCTCTTCGT
    CCTGCTAACA CCATTACCAA GGTCCCTTCA GAGAAGATCC TCAGAGCTGG AAAAATTTTA
    CGAAATGCCA TTCTCTCTCG AGCACCTCAC ATGATAAGAG ATAGAAAATA CCACCTAAAG
    ACATACAGAC AATGCTGTGT GGGAACTGAA CTGGTGGACT GGATGATGCA GCAGACACCA
    TGTGTTCACT CCCGGACTCA AGCTGTTGGC ATGTGGCAAG TCCTGTTAGA AGATGGTGTT
    CTCAACCACG TGGACCAGGA GCACCATTTC CAAGACAAAT ATTTATTCTA TCGATTTCTG
    GATGATGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGACGCCCA CATGAGGATG
    ATCCTTCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAGATTAT CTATGAGGAG
    CTTCTTCATA TTAAAGCCTT ATCCCATCTT TCTACCACAG TGAAACGAGA GTTAGCAGGT
    GTTCTCATTT TTGAGTCTCA CGCCAAAGGA GGGACTGTGT TGTTTAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCAGTGAATG TAGTCATTTA CGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA AGGAGATGAC TTCGGCAAGT TAGCACTAGT GAATGATGCC
    CCACGAGCTG CCTCTATCGT CTTACGAGAA GATAACTGCC ATTTCTTAAG AGTAGACAAG
    GAGGATTTCA ACCGGATCCT AAGGGACGTG GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGAGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGGCA ACTTTAAATG AAGCAACAGA TTCTGTTTTA
    AATGACTTTA TTATGATGCA CTGTGTTTTT ATGCCAAATA CCCAGCTTTG CCCGGCACTG
    GTGGCCCACT ACCACGCACA GCCTTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGGCGAGT CATCCGCCTG GTTCTACAGT GGGCTGCCAT GTATGGAGAC
    CTCCTGCAAG AGGATGACGT GTCTATGGCC TTCCTGGAGG AGTTTTATGT ATCTGTATCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CAGAGTTGGA GAAGATTGTC
    AAGCAAATCT CAGAAGATGC AAAGGCACCA CAAAAGAAGC ACAAGGTTCT TTTGCAACAG
    TTCAATACGG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGCGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC AGTGGCCACT
    TCGGTGAAGG AAGTCATCAG TGCAGTTGCC GACAAGCTGG GCTCCGGGGA GGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACGACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGATTCA
    CTGACTCCCT TACCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGC
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGGAGATTT AATGAAATTC AGTTTTGGGT CGTCACTGAG
    ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT GTTCAGCTAT TAAAAAAATT TATTAAGATA
    GCAGCCCACT GTAAGGAGTA TAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAACGTTG CTGTGAGCCG CTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGTTTAATG GACCCTTCAA GGAACCACAG GGCCTACAGG
    CTGACAGTAG CTAAGCTGGA ACCTCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACG TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC GGCCAGAACA GTGAGATACT ACAGGAGCCA ACCCTTCAAT
    CCTGATGCAG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109023
    Clone ID Related Accession (Same CDS sequence) NM_001375868.1
    Accession Version NM_001375868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform j
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.28730.1, SRR1803616.87116.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGAA AAGGTGGTGC TCAAACCTAA TGATGTTTCA
    GTATTTACGA CGCTCACCAT TAATGGACGC CTGTTTGCTT GCCCGCGAGA GCAATTCGAT
    TCACTGACTC CCTTACCAGA ACAGGAAGGC CCAACTGTTG GAACAGTGGG AACTTTTGAA
    CTGATGAGCT CCAAAGATTT AGCATACCAG ATGACAATTT ATGATTGGGA ACTCTTCAAC
    TGCGTGCATG AGCTGGAGCT AATCTATCAC ACATTTGGAA GGCATAATTT TAAAAAGACC
    ACAGCAAACT TGGATTTGTT CCTGAGGAGA TTTAATGAAA TTCAGTTTTG GGTCGTCACT
    GAGATCTGCC TTTGTTCTCA GCTCAGCAAG CGTGTTCAGC TATTAAAAAA ATTTATTAAG
    ATAGCAGCCC ACTGTAAGGA GTATAAAAAT CTGAATTCCT TTTTTGCCAT CGTCATGGGA
    CTAAGTAACG TTGCTGTGAG CCGCTTGGCA CTAACGTGGG AGAAACTGCC AAGCAAGTTC
    AAGAAGTTCT ATGCGGAGTT TGAAAGTTTA ATGGACCCTT CAAGGAACCA CAGGGCCTAC
    AGGCTGACAG TAGCTAAGCT GGAACCTCCT CTCATCCCCT TCATGCCTTT GCTCATTAAA
    GATATGACAT TTACTCATGA GGGGAACAAG ACGTTCATTG ACAATCTAGT AAACTTTGAA
    AAAATGCGCA TGATTGCAAA TACGGCCAGA ACAGTGAGAT ACTACAGGAG CCAACCCTTC
    AATCCTGATG CAGCTCAAGC TAATAAGAAC CATCAGGATG TCCGGAGTTA TGTACGGCAA
    TTAAATGTGA TTGACAACCA GAGAACTTTA TCACAGATGT CACACAGATT AGAGCCTCGT
    CGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362797.1
    CDS140..3028
    Translation

    Target ORF information:

    RefSeq Version NM_001375868.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGAA AAGGTGGTGC TCAAACCTAA TGATGTTTCA
    GTATTTACGA CGCTCACCAT TAATGGACGC CTGTTTGCTT GCCCGCGAGA GCAATTCGAT
    TCACTGACTC CCTTACCAGA ACAGGAAGGC CCAACTGTTG GAACAGTGGG AACTTTTGAA
    CTGATGAGCT CCAAAGATTT AGCATACCAG ATGACAATTT ATGATTGGGA ACTCTTCAAC
    TGCGTGCATG AGCTGGAGCT AATCTATCAC ACATTTGGAA GGCATAATTT TAAAAAGACC
    ACAGCAAACT TGGATTTGTT CCTGAGGAGA TTTAATGAAA TTCAGTTTTG GGTCGTCACT
    GAGATCTGCC TTTGTTCTCA GCTCAGCAAG CGTGTTCAGC TATTAAAAAA ATTTATTAAG
    ATAGCAGCCC ACTGTAAGGA GTATAAAAAT CTGAATTCCT TTTTTGCCAT CGTCATGGGA
    CTAAGTAACG TTGCTGTGAG CCGCTTGGCA CTAACGTGGG AGAAACTGCC AAGCAAGTTC
    AAGAAGTTCT ATGCGGAGTT TGAAAGTTTA ATGGACCCTT CAAGGAACCA CAGGGCCTAC
    AGGCTGACAG TAGCTAAGCT GGAACCTCCT CTCATCCCCT TCATGCCTTT GCTCATTAAA
    GATATGACAT TTACTCATGA GGGGAACAAG ACGTTCATTG ACAATCTAGT AAACTTTGAA
    AAAATGCGCA TGATTGCAAA TACGGCCAGA ACAGTGAGAT ACTACAGGAG CCAACCCTTC
    AATCCTGATG CAGCTCAAGC TAATAAGAAC CATCAGGATG TCCGGAGTTA TGTACGGCAA
    TTAAATGTGA TTGACAACCA GAGAACTTTA TCACAGATGT CACACAGATT AGAGCCTCGT
    CGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109024
    Clone ID Related Accession (Same CDS sequence) NM_001375872.1
    Accession Version NM_001375872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform n
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.61449.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGC GCATGATTGC AAATACGGCC AGAACAGTGA GATACTACAG GAGCCAACCC
    TTCAATCCTG ATGCAGCTCA AGCTAATAAG AACCATCAGG ATGTCCGGAG TTATGTACGG
    CAATTAAATG TGATTGACAA CCAGAGAACT TTATCACAGA TGTCACACAG ATTAGAGCCT
    CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362801.1
    CDS140..2971
    Translation

    Target ORF information:

    RefSeq Version NM_001375872.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGC GCATGATTGC AAATACGGCC AGAACAGTGA GATACTACAG GAGCCAACCC
    TTCAATCCTG ATGCAGCTCA AGCTAATAAG AACCATCAGG ATGTCCGGAG TTATGTACGG
    CAATTAAATG TGATTGACAA CCAGAGAACT TTATCACAGA TGTCACACAG ATTAGAGCCT
    CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109027
    Clone ID Related Accession (Same CDS sequence) NM_001375870.1
    Accession Version NM_001375870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform l
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.8360.1, SRR1803615.264136.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGCG CATGATTGCA AATACGGCCA GAACAGTGAG
    ATACTACAGG AGCCAACCCT TCAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362799.1
    CDS140..2989
    Translation

    Target ORF information:

    RefSeq Version NM_001375870.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGCG CATGATTGCA AATACGGCCA GAACAGTGAG
    ATACTACAGG AGCCAACCCT TCAATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109028
    Clone ID Related Accession (Same CDS sequence) NM_001375874.1
    Accession Version NM_001375874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform p
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.47890.1, SRR1803614.116830.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGCGCATG ATTGCAAATA CGGCCAGAAC
    AGTGAGATAC TACAGGAGCC AACCCTTCAA TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362803.1
    CDS140..2935
    Translation

    Target ORF information:

    RefSeq Version NM_001375874.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGCGCATG ATTGCAAATA CGGCCAGAAC
    AGTGAGATAC TACAGGAGCC AACCCTTCAA TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109029
    Clone ID Related Accession (Same CDS sequence) NM_001375869.1
    Accession Version NM_001375869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform k
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.76217.1, SRR1803611.229882.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362798.1
    CDS140..3022
    Translation

    Target ORF information:

    RefSeq Version NM_001375869.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109030
    Clone ID Related Accession (Same CDS sequence) NM_001375871.1
    Accession Version NM_001375871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform m
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.12033.1, SRR1803617.4977.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGAAAAGG TGGTGCTCAA ACCTAATGAT
    GTTTCAGTAT TTACGACGCT CACCATTAAT GGACGCCTGT TTGCTTGCCC GCGAGAGCAA
    TTCGATTCAC TGACTCCCTT ACCAGAACAG GAAGGCCCAA CTGTTGGAAC AGTGGGAACT
    TTTGAACTGA TGAGCTCCAA AGATTTAGCA TACCAGATGA CAATTTATGA TTGGGAACTC
    TTCAACTGCG TGCATGAGCT GGAGCTAATC TATCACACAT TTGGAAGGCA TAATTTTAAA
    AAGACCACAG CAAACTTGGA TTTGTTCCTG AGGAGATTTA ATGAAATTCA GTTTTGGGTC
    GTCACTGAGA TCTGCCTTTG TTCTCAGCTC AGCAAGCGTG TTCAGCTATT AAAAAAATTT
    ATTAAGATAG CAGCCCACTG TAAGGAGTAT AAAAATCTGA ATTCCTTTTT TGCCATCGTC
    ATGGGACTAA GTAACGTTGC TGTGAGCCGC TTGGCACTAA CGTGGGAGAA ACTGCCAAGC
    AAGTTCAAGA AGTTCTATGC GGAGTTTGAA AGTTTAATGG ACCCTTCAAG GAACCACAGG
    GCCTACAGGC TGACAGTAGC TAAGCTGGAA CCTCCTCTCA TCCCCTTCAT GCCTTTGCTC
    ATTAAAGATA TGACATTTAC TCATGAGGGG AACAAGACGT TCATTGACAA TCTAGTAAAC
    TTTGAAAAAA TGCGCATGAT TGCAAATACG GCCAGAACAG TGAGATACTA CAGGAGCCAA
    CCCTTCAATC CTGATGCAGC TCAAGCTAAT AAGAACCATC AGGATGTCCG GAGTTATGTA
    CGGCAATTAA ATGTGATTGA CAACCAGAGA ACTTTATCAC AGATGTCACA CAGATTAGAG
    CCTCGTCGAC CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362800.1
    CDS140..2974
    Translation

    Target ORF information:

    RefSeq Version NM_001375871.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGAAAAGG TGGTGCTCAA ACCTAATGAT
    GTTTCAGTAT TTACGACGCT CACCATTAAT GGACGCCTGT TTGCTTGCCC GCGAGAGCAA
    TTCGATTCAC TGACTCCCTT ACCAGAACAG GAAGGCCCAA CTGTTGGAAC AGTGGGAACT
    TTTGAACTGA TGAGCTCCAA AGATTTAGCA TACCAGATGA CAATTTATGA TTGGGAACTC
    TTCAACTGCG TGCATGAGCT GGAGCTAATC TATCACACAT TTGGAAGGCA TAATTTTAAA
    AAGACCACAG CAAACTTGGA TTTGTTCCTG AGGAGATTTA ATGAAATTCA GTTTTGGGTC
    GTCACTGAGA TCTGCCTTTG TTCTCAGCTC AGCAAGCGTG TTCAGCTATT AAAAAAATTT
    ATTAAGATAG CAGCCCACTG TAAGGAGTAT AAAAATCTGA ATTCCTTTTT TGCCATCGTC
    ATGGGACTAA GTAACGTTGC TGTGAGCCGC TTGGCACTAA CGTGGGAGAA ACTGCCAAGC
    AAGTTCAAGA AGTTCTATGC GGAGTTTGAA AGTTTAATGG ACCCTTCAAG GAACCACAGG
    GCCTACAGGC TGACAGTAGC TAAGCTGGAA CCTCCTCTCA TCCCCTTCAT GCCTTTGCTC
    ATTAAAGATA TGACATTTAC TCATGAGGGG AACAAGACGT TCATTGACAA TCTAGTAAAC
    TTTGAAAAAA TGCGCATGAT TGCAAATACG GCCAGAACAG TGAGATACTA CAGGAGCCAA
    CCCTTCAATC CTGATGCAGC TCAAGCTAAT AAGAACCATC AGGATGTCCG GAGTTATGTA
    CGGCAATTAA ATGTGATTGA CAACCAGAGA ACTTTATCAC AGATGTCACA CAGATTAGAG
    CCTCGTCGAC CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109032
    Clone ID Related Accession (Same CDS sequence) NM_001375867.1
    Accession Version NM_001375867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform i
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.55442.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGACACCT CTCATTGAAC CTCACGTTCC TCTTCGTCCT
    GCTAACACCA TTACCAAGGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362796.1
    CDS140..3082
    Translation

    Target ORF information:

    RefSeq Version NM_001375867.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGACACCT CTCATTGAAC CTCACGTTCC TCTTCGTCCT
    GCTAACACCA TTACCAAGGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109033
    Clone ID Related Accession (Same CDS sequence) NM_001375873.1
    Accession Version NM_001375873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform o
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.191354.1, SRR1803617.28479.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGCGCA TGATTGCAAA TACGGCCAGA ACAGTGAGAT ACTACAGGAG CCAACCCTTC
    AATCCTGATG CAGCTCAAGC TAATAAGAAC CATCAGGATG TCCGGAGTTA TGTACGGCAA
    TTAAATGTGA TTGACAACCA GAGAACTTTA TCACAGATGT CACACAGATT AGAGCCTCGT
    CGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362802.1
    CDS140..2968
    Translation

    Target ORF information:

    RefSeq Version NM_001375873.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGCGCA TGATTGCAAA TACGGCCAGA ACAGTGAGAT ACTACAGGAG CCAACCCTTC
    AATCCTGATG CAGCTCAAGC TAATAAGAAC CATCAGGATG TCCGGAGTTA TGTACGGCAA
    TTAAATGTGA TTGACAACCA GAGAACTTTA TCACAGATGT CACACAGATT AGAGCCTCGT
    CGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17215
    Clone ID Related Accession (Same CDS sequence) NM_001100397.1 , NM_001100397.2
    Accession Version NM_001100397.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC009484.3, AC104086.6 and AC019046.4. On Nov 5, 2019 this sequence version replaced NM_001100397.1. Transcript Variant: This variant (2) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040183.2, AK304278.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCTACA AGAAATATCG ACAGTATATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TCCCTTCAGA GAAGATCCTC
    AGAGCTGGAA AAATTTTACG AAATGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAA TGCTGTGTGG GAACTGAACT GGTGGACTGG
    ATGATGCAGC AGACACCATG TGTTCACTCC CGGACTCAAG CTGTTGGCAT GTGGCAAGTC
    CTGTTAGAAG ATGGTGTTCT CAACCACGTG GACCAGGAGC ACCATTTCCA AGACAAATAT
    TTATTCTATC GATTTCTGGA TGATGAGCAC GAGGATGCCC CTTTGCCTAC TGAGGAGGAG
    AAGAAGGAGT GTGATGAGGA GCTCCAGGAC ACCATGCTGC TGCTGTCACA GATGGGCCCC
    GACGCCCACA TGAGGATGAT CCTTCGCAAA CCACCTGGCC AGAGGACTGT GGATGACCTA
    GAGATTATCT ATGAGGAGCT TCTTCATATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGAGAGT TAGCAGGTGT TCTCATTTTT GAGTCTCACG CCAAAGGAGG GACTGTGTTG
    TTTAACCAGG GGGAAGAAGG TACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTACG GCAAGGGTGT GGTCTGCACC CTGCATGAAG GAGATGACTT CGGCAAGTTA
    GCACTAGTGA ATGATGCCCC ACGAGCTGCC TCTATCGTCT TACGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTAA GGGACGTGGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGAGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACAATACGCC TTGAGGCAAC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTATT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CGGCACTGGT GGCCCACTAC CACGCACAGC CTTCACAAGG TACAGAACAG
    GAGAAAATGG ATTATGCCCT CAACAATAAG AGGCGAGTCA TCCGCCTGGT TCTACAGTGG
    GCTGCCATGT ATGGAGACCT CCTGCAAGAG GATGACGTGT CTATGGCCTT CCTGGAGGAG
    TTTTATGTAT CTGTATCAGA TGATGCCCGG ATGATTGCTG CCCTCAAGGA GCAACTGCCA
    GAGTTGGAGA AGATTGTCAA GCAAATCTCA GAAGATGCAA AGGCACCACA AAAGAAGCAC
    AAGGTTCTTT TGCAACAGTT CAATACGGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGCGGCTCTG ATGAAGTTCT GTTTAAGGTC TATTGCATGG ACCACACCTA CACAACCATT
    CGGGTGCCAG TGGCCACTTC GGTGAAGGAA GTCATCAGTG CAGTTGCCGA CAAGCTGGGC
    TCCGGGGAGG GCCTGATCAT AGTCAAGATG AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACGACGCTC ACCATTAATG GACGCCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTA CCAGAACAGG AAGGCCCAAC TGTTGGAACA
    GTGGGAACTT TTGAACTGAT GAGCTCCAAA GATTTAGCAT ACCAGATGAC AATTTATGAT
    TGGGAACTCT TCAACTGCGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    AATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGATTTAA TGAAATTCAG
    TTTTGGGTCG TCACTGAGAT CTGCCTTTGT TCTCAGCTCA GCAAGCGTGT TCAGCTATTA
    AAAAAATTTA TTAAGATAGC AGCCCACTGT AAGGAGTATA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAACGTTGCT GTGAGCCGCT TGGCACTAAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCG GAGTTTGAAA GTTTAATGGA CCCTTCAAGG
    AACCACAGGG CCTACAGGCT GACAGTAGCT AAGCTGGAAC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTAGTAAACT TTGAAAAAAT GCGCATGATT GCAAATACGG CCAGAACAGT GAGATACTAC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCATCA GGATGTCCGG
    AGTTATGTAC GGCAATTAAA TGTGATTGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093867.1
    CDS141..2744
    Translation

    Target ORF information:

    RefSeq Version NM_001100397.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100397.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCTACA AGAAATATCG ACAGTATATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TCCCTTCAGA GAAGATCCTC
    AGAGCTGGAA AAATTTTACG AAATGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAA TGCTGTGTGG GAACTGAACT GGTGGACTGG
    ATGATGCAGC AGACACCATG TGTTCACTCC CGGACTCAAG CTGTTGGCAT GTGGCAAGTC
    CTGTTAGAAG ATGGTGTTCT CAACCACGTG GACCAGGAGC ACCATTTCCA AGACAAATAT
    TTATTCTATC GATTTCTGGA TGATGAGCAC GAGGATGCCC CTTTGCCTAC TGAGGAGGAG
    AAGAAGGAGT GTGATGAGGA GCTCCAGGAC ACCATGCTGC TGCTGTCACA GATGGGCCCC
    GACGCCCACA TGAGGATGAT CCTTCGCAAA CCACCTGGCC AGAGGACTGT GGATGACCTA
    GAGATTATCT ATGAGGAGCT TCTTCATATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGAGAGT TAGCAGGTGT TCTCATTTTT GAGTCTCACG CCAAAGGAGG GACTGTGTTG
    TTTAACCAGG GGGAAGAAGG TACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTACG GCAAGGGTGT GGTCTGCACC CTGCATGAAG GAGATGACTT CGGCAAGTTA
    GCACTAGTGA ATGATGCCCC ACGAGCTGCC TCTATCGTCT TACGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTAA GGGACGTGGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGAGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACAATACGCC TTGAGGCAAC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTATT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CGGCACTGGT GGCCCACTAC CACGCACAGC CTTCACAAGG TACAGAACAG
    GAGAAAATGG ATTATGCCCT CAACAATAAG AGGCGAGTCA TCCGCCTGGT TCTACAGTGG
    GCTGCCATGT ATGGAGACCT CCTGCAAGAG GATGACGTGT CTATGGCCTT CCTGGAGGAG
    TTTTATGTAT CTGTATCAGA TGATGCCCGG ATGATTGCTG CCCTCAAGGA GCAACTGCCA
    GAGTTGGAGA AGATTGTCAA GCAAATCTCA GAAGATGCAA AGGCACCACA AAAGAAGCAC
    AAGGTTCTTT TGCAACAGTT CAATACGGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGCGGCTCTG ATGAAGTTCT GTTTAAGGTC TATTGCATGG ACCACACCTA CACAACCATT
    CGGGTGCCAG TGGCCACTTC GGTGAAGGAA GTCATCAGTG CAGTTGCCGA CAAGCTGGGC
    TCCGGGGAGG GCCTGATCAT AGTCAAGATG AGTTCCGGAG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACGACGCTC ACCATTAATG GACGCCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTA CCAGAACAGG AAGGCCCAAC TGTTGGAACA
    GTGGGAACTT TTGAACTGAT GAGCTCCAAA GATTTAGCAT ACCAGATGAC AATTTATGAT
    TGGGAACTCT TCAACTGCGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    AATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGATTTAA TGAAATTCAG
    TTTTGGGTCG TCACTGAGAT CTGCCTTTGT TCTCAGCTCA GCAAGCGTGT TCAGCTATTA
    AAAAAATTTA TTAAGATAGC AGCCCACTGT AAGGAGTATA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAACGTTGCT GTGAGCCGCT TGGCACTAAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCG GAGTTTGAAA GTTTAATGGA CCCTTCAAGG
    AACCACAGGG CCTACAGGCT GACAGTAGCT AAGCTGGAAC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTAGTAAACT TTGAAAAAAT GCGCATGATT GCAAATACGG CCAGAACAGT GAGATACTAC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCATCA GGATGTCCGG
    AGTTATGTAC GGCAATTAAA TGTGATTGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGATTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20982
    Clone ID Related Accession (Same CDS sequence) NM_001282900.2 , NM_001282900.1 , XM_006712205.3
    Accession Version NM_001282900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform d
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC009484.3, AC104086.6 and AC019046.4. On Nov 5, 2019 this sequence version replaced NM_001282900.1. Transcript Variant: This variant (4) differs in its 5' UTR, lacks several exons in the 5' coding region, initiates translation at a downstream in-frame start codon, and lacks an additional in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296340.1, SRR1660805.207914.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269829.1
    CDS174..2696
    Translation

    Target ORF information:

    RefSeq Version NM_001282900.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT CCCTTCAGAG AAGATCCTCA GAGCTGGAAA AATTTTACGA
    AATGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAAT GCTGTGTGGG AACTGAACTG GTGGACTGGA TGATGCAGCA GACACCATGT
    GTTCACTCCC GGACTCAAGC TGTTGGCATG TGGCAAGTCC TGTTAGAAGA TGGTGTTCTC
    AACCACGTGG ACCAGGAGCA CCATTTCCAA GACAAATATT TATTCTATCG ATTTCTGGAT
    GATGAGCACG AGGATGCCCC TTTGCCTACT GAGGAGGAGA AGAAGGAGTG TGATGAGGAG
    CTCCAGGACA CCATGCTGCT GCTGTCACAG ATGGGCCCCG ACGCCCACAT GAGGATGATC
    CTTCGCAAAC CACCTGGCCA GAGGACTGTG GATGACCTAG AGATTATCTA TGAGGAGCTT
    CTTCATATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGAGAGTT AGCAGGTGTT
    CTCATTTTTG AGTCTCACGC CAAAGGAGGG ACTGTGTTGT TTAACCAGGG GGAAGAAGGT
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTACGG CAAGGGTGTG
    GTCTGCACCC TGCATGAAGG AGATGACTTC GGCAAGTTAG CACTAGTGAA TGATGCCCCA
    CGAGCTGCCT CTATCGTCTT ACGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTAAG GGACGTGGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GAGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CAATACGCCT TGAGGCAACT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTATTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC GGCACTGGTG
    GCCCACTACC ACGCACAGCC TTCACAAGGT ACAGAACAGG AGAAAATGGA TTATGCCCTC
    AACAATAAGA GGCGAGTCAT CCGCCTGGTT CTACAGTGGG CTGCCATGTA TGGAGACCTC
    CTGCAAGAGG ATGACGTGTC TATGGCCTTC CTGGAGGAGT TTTATGTATC TGTATCAGAT
    GATGCCCGGA TGATTGCTGC CCTCAAGGAG CAACTGCCAG AGTTGGAGAA GATTGTCAAG
    CAAATCTCAG AAGATGCAAA GGCACCACAA AAGAAGCACA AGGTTCTTTT GCAACAGTTC
    AATACGGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GCGGCTCTGA TGAAGTTCTG
    TTTAAGGTCT ATTGCATGGA CCACACCTAC ACAACCATTC GGGTGCCAGT GGCCACTTCG
    GTGAAGGAAG TCATCAGTGC AGTTGCCGAC AAGCTGGGCT CCGGGGAGGG CCTGATCATA
    GTCAAGATGA GTTCCGGAGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21563
    Clone ID Related Accession (Same CDS sequence) NM_001282901.1 , NM_001282901.2
    Accession Version NM_001282901.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-07
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform e
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC009484.3, AC104086.6 and AC019046.4. On Nov 8, 2019 this sequence version replaced NM_001282901.1. Transcript Variant: This variant (5) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (e) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294433.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA 
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269830.1
    CDS265..2640
    Translation

    Target ORF information:

    RefSeq Version NM_001282901.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282901.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA 
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21628
    Clone ID Related Accession (Same CDS sequence) NM_001282899.1 , NM_001282899.2
    Accession Version NM_001282899.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC009484.3, AC104086.6 and AC019046.4. On Nov 5, 2019 this sequence version replaced NM_001282899.1. Transcript Variant: This variant (3) differs in its 5' UTR, lacks several exons in the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316072.1, AK316014.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGAGCTG GAAAAATTTT ACGAAATGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAATGCTGTG TGGGAACTGA ACTGGTGGAC TGGATGATGC AGCAGACACC ATGTGTTCAC
    TCCCGGACTC AAGCTGTTGG CATGTGGCAA GTCCTGTTAG AAGATGGTGT TCTCAACCAC
    GTGGACCAGG AGCACCATTT CCAAGACAAA TATTTATTCT ATCGATTTCT GGATGATGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGACGCCC ACATGAGGAT GATCCTTCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAGATTA TCTATGAGGA GCTTCTTCAT
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGAG AGTTAGCAGG TGTTCTCATT
    TTTGAGTCTC ACGCCAAAGG AGGGACTGTG TTGTTTAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ACGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AAGGAGATGA CTTCGGCAAG TTAGCACTAG TGAATGATGC CCCACGAGCT
    GCCTCTATCG TCTTACGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TAAGGGACGT GGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGAGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGGC AACTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    ATTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCGGCACT GGTGGCCCAC
    TACCACGCAC AGCCTTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGGCGAG TCATCCGCCT GGTTCTACAG TGGGCTGCCA TGTATGGAGA CCTCCTGCAA
    GAGGATGACG TGTCTATGGC CTTCCTGGAG GAGTTTTATG TATCTGTATC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCAGAGTTGG AGAAGATTGT CAAGCAAATC
    TCAGAAGATG CAAAGGCACC ACAAAAGAAG CACAAGGTTC TTTTGCAACA GTTCAATACG
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGCGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CAGTGGCCAC TTCGGTGAAG
    GAAGTCATCA GTGCAGTTGC CGACAAGCTG GGCTCCGGGG AGGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACGACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTACCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG CGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGATT TAATGAAATT CAGTTTTGGG TCGTCACTGA GATCTGCCTT
    TGTTCTCAGC TCAGCAAGCG TGTTCAGCTA TTAAAAAAAT TTATTAAGAT AGCAGCCCAC
    TGTAAGGAGT ATAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAACGTT
    GCTGTGAGCC GCTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGTTTAAT GGACCCTTCA AGGAACCACA GGGCCTACAG GCTGACAGTA
    GCTAAGCTGG AACCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CGGCCAGAAC AGTGAGATAC TACAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269828.1
    CDS174..2750
    Translation

    Target ORF information:

    RefSeq Version NM_001282899.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282899.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTCCCTTC AGAGAAGATC CTCAGAGCTG GAAAAATTTT ACGAAATGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAATGCTGTG TGGGAACTGA ACTGGTGGAC TGGATGATGC AGCAGACACC ATGTGTTCAC
    TCCCGGACTC AAGCTGTTGG CATGTGGCAA GTCCTGTTAG AAGATGGTGT TCTCAACCAC
    GTGGACCAGG AGCACCATTT CCAAGACAAA TATTTATTCT ATCGATTTCT GGATGATGAG
    CACGAGGATG CCCCTTTGCC TACTGAGGAG GAGAAGAAGG AGTGTGATGA GGAGCTCCAG
    GACACCATGC TGCTGCTGTC ACAGATGGGC CCCGACGCCC ACATGAGGAT GATCCTTCGC
    AAACCACCTG GCCAGAGGAC TGTGGATGAC CTAGAGATTA TCTATGAGGA GCTTCTTCAT
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGAG AGTTAGCAGG TGTTCTCATT
    TTTGAGTCTC ACGCCAAAGG AGGGACTGTG TTGTTTAACC AGGGGGAAGA AGGTACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ACGGCAAGGG TGTGGTCTGC
    ACCCTGCATG AAGGAGATGA CTTCGGCAAG TTAGCACTAG TGAATGATGC CCCACGAGCT
    GCCTCTATCG TCTTACGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TAAGGGACGT GGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGAGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACAATAC GCCTTGAGGC AACTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    ATTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCGGCACT GGTGGCCCAC
    TACCACGCAC AGCCTTCACA AGGTACAGAA CAGGAGAAAA TGGATTATGC CCTCAACAAT
    AAGAGGCGAG TCATCCGCCT GGTTCTACAG TGGGCTGCCA TGTATGGAGA CCTCCTGCAA
    GAGGATGACG TGTCTATGGC CTTCCTGGAG GAGTTTTATG TATCTGTATC AGATGATGCC
    CGGATGATTG CTGCCCTCAA GGAGCAACTG CCAGAGTTGG AGAAGATTGT CAAGCAAATC
    TCAGAAGATG CAAAGGCACC ACAAAAGAAG CACAAGGTTC TTTTGCAACA GTTCAATACG
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGCGGCT CTGATGAAGT TCTGTTTAAG
    GTCTATTGCA TGGACCACAC CTACACAACC ATTCGGGTGC CAGTGGCCAC TTCGGTGAAG
    GAAGTCATCA GTGCAGTTGC CGACAAGCTG GGCTCCGGGG AGGGCCTGAT CATAGTCAAG
    ATGAGTTCCG GAGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACGACG
    CTCACCATTA ATGGACGCCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTACCAGAAC AGGAAGGCCC AACTGTTGGA ACAGTGGGAA CTTTTGAACT GATGAGCTCC
    AAAGATTTAG CATACCAGAT GACAATTTAT GATTGGGAAC TCTTCAACTG CGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATAATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGATT TAATGAAATT CAGTTTTGGG TCGTCACTGA GATCTGCCTT
    TGTTCTCAGC TCAGCAAGCG TGTTCAGCTA TTAAAAAAAT TTATTAAGAT AGCAGCCCAC
    TGTAAGGAGT ATAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAACGTT
    GCTGTGAGCC GCTTGGCACT AACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCGGAGTTTG AAAGTTTAAT GGACCCTTCA AGGAACCACA GGGCCTACAG GCTGACAGTA
    GCTAAGCTGG AACCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTAGTAA ACTTTGAAAA AATGCGCATG
    ATTGCAAATA CGGCCAGAAC AGTGAGATAC TACAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA TCAGGATGTC CGGAGTTATG TACGGCAATT AAATGTGATT
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGATTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35775
    Clone ID Related Accession (Same CDS sequence) XM_005246246.3 , XM_005246246.2 , NM_001375866.1
    Accession Version NM_001375866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform h
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. On Nov 5, 2019 this sequence version replaced XM_005246246.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KJ534925.1, SRR1660809.217188.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACGGCC
    AGAACAGTGA GATACTACAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362795.1
    CDS140..3121
    Translation

    Target ORF information:

    RefSeq Version NM_001375866.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTC
    CCTTCAGAGA AGATCCTCAG AGCTGGAAAA ATTTTACGAA ATGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAATG CTGTGTGGGA
    ACTGAACTGG TGGACTGGAT GATGCAGCAG ACACCATGTG TTCACTCCCG GACTCAAGCT
    GTTGGCATGT GGCAAGTCCT GTTAGAAGAT GGTGTTCTCA ACCACGTGGA CCAGGAGCAC
    CATTTCCAAG ACAAATATTT ATTCTATCGA TTTCTGGATG ATGAGCACGA GGATGCCCCT
    TTGCCTACTG AGGAGGAGAA GAAGGAGTGT GATGAGGAGC TCCAGGACAC CATGCTGCTG
    CTGTCACAGA TGGGCCCCGA CGCCCACATG AGGATGATCC TTCGCAAACC ACCTGGCCAG
    AGGACTGTGG ATGACCTAGA GATTATCTAT GAGGAGCTTC TTCATATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGAGAGTTA GCAGGTGTTC TCATTTTTGA GTCTCACGCC
    AAAGGAGGGA CTGTGTTGTT TAACCAGGGG GAAGAAGGTA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTACGGC AAGGGTGTGG TCTGCACCCT GCATGAAGGA
    GATGACTTCG GCAAGTTAGC ACTAGTGAAT GATGCCCCAC GAGCTGCCTC TATCGTCTTA
    CGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTAAGG
    GACGTGGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG AGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC AATACGCCTT
    GAGGCAACTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTATTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCG GCACTGGTGG CCCACTACCA CGCACAGCCT
    TCACAAGGTA CAGAACAGGA GAAAATGGAT TATGCCCTCA ACAATAAGAG GCGAGTCATC
    CGCCTGGTTC TACAGTGGGC TGCCATGTAT GGAGACCTCC TGCAAGAGGA TGACGTGTCT
    ATGGCCTTCC TGGAGGAGTT TTATGTATCT GTATCAGATG ATGCCCGGAT GATTGCTGCC
    CTCAAGGAGC AACTGCCAGA GTTGGAGAAG ATTGTCAAGC AAATCTCAGA AGATGCAAAG
    GCACCACAAA AGAAGCACAA GGTTCTTTTG CAACAGTTCA ATACGGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG CGGCTCTGAT GAAGTTCTGT TTAAGGTCTA TTGCATGGAC
    CACACCTACA CAACCATTCG GGTGCCAGTG GCCACTTCGG TGAAGGAAGT CATCAGTGCA
    GTTGCCGACA AGCTGGGCTC CGGGGAGGGC CTGATCATAG TCAAGATGAG TTCCGGAGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CGACGCTCAC CATTAATGGA
    CGCCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTACC AGAACAGGAA
    GGCCCAACTG TTGGAACAGT GGGAACTTTT GAACTGATGA GCTCCAAAGA TTTAGCATAC
    CAGATGACAA TTTATGATTG GGAACTCTTC AACTGCGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATAA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGATTTAATG AAATTCAGTT TTGGGTCGTC ACTGAGATCT GCCTTTGTTC TCAGCTCAGC
    AAGCGTGTTC AGCTATTAAA AAAATTTATT AAGATAGCAG CCCACTGTAA GGAGTATAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ACGTTGCTGT GAGCCGCTTG
    GCACTAACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCGGA GTTTGAAAGT
    TTAATGGACC CTTCAAGGAA CCACAGGGCC TACAGGCTGA CAGTAGCTAA GCTGGAACCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT AGTAAACTTT GAAAAAATGC GCATGATTGC AAATACGGCC
    AGAACAGTGA GATACTACAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCATCAGG ATGTCCGGAG TTATGTACGG CAATTAAATG TGATTGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ATTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88944
    Clone ID Related Accession (Same CDS sequence) XM_017003196.1 , XM_017003196.2
    Accession Version XM_017003196.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCCTGTTG CTGAAATGCA CAAATGTTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858685.1
    CDS55..2937
    Translation

    Target ORF information:

    RefSeq Version XM_017003196.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCCTGTTG CTGAAATGCA CAAATGTTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88944
    Clone ID Related Accession (Same CDS sequence) XM_017003196.1 , XM_017003196.2
    Accession Version XM_017003196.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCCTGTTG CTGAAATGCA CAAATGTTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858685.1
    CDS59..2941
    Translation

    Target ORF information:

    RefSeq Version XM_017003196.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003196.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCCTGTTG CTGAAATGCA CAAATGTTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88945
    Clone ID Related Accession (Same CDS sequence) XM_017003197.1
    Accession Version XM_017003197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTCTACA AGAAATATCG ACAGTATATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACGTCCC TTCAGAGAAG
    ATCCTCAGAG CTGGAAAAAT TTTACGAAAT GCCATTCTCT CTCGAGCACC TCACATGATA
    AGAGATAGAA AATACCACCT AAAGACATAC AGACAATGCT GTGTGGGAAC TGAACTGGTG
    GACTGGATGA TGCAGCAGAC ACCATGTGTT CACTCCCGGA CTCAAGCTGT TGGCATGTGG
    CAAGTCCTGT TAGAAGATGG TGTTCTCAAC CACGTGGACC AGGAGCACCA TTTCCAAGAC
    AAATATTTAT TCTATCGATT TCTGGATGAT GAGCACGAGG ATGCCCCTTT GCCTACTGAG
    GAGGAGAAGA AGGAGTGTGA TGAGGAGCTC CAGGACACCA TGCTGCTGCT GTCACAGATG
    GGCCCCGACG CCCACATGAG GATGATCCTT CGCAAACCAC CTGGCCAGAG GACTGTGGAT
    GACCTAGAGA TTATCTATGA GGAGCTTCTT CATATTAAAG CCTTATCCCA TCTTTCTACC
    ACAGTGAAAC GAGAGTTAGC AGGTGTTCTC ATTTTTGAGT CTCACGCCAA AGGAGGGACT
    GTGTTGTTTA ACCAGGGGGA AGAAGGTACC TCCTGGTACA TTATTCTAAA AGGATCAGTG
    AATGTAGTCA TTTACGGCAA GGGTGTGGTC TGCACCCTGC ATGAAGGAGA TGACTTCGGC
    AAGTTAGCAC TAGTGAATGA TGCCCCACGA GCTGCCTCTA TCGTCTTACG AGAAGATAAC
    TGCCATTTCT TAAGAGTAGA CAAGGAGGAT TTCAACCGGA TCCTAAGGGA CGTGGAGGCG
    AATACAGTCA GACTTAAAGA ACATGACCAA GATGTCTTGG TGCTGGAGAA GGTCCCAGCA
    GGGAACAGAG CTTCTAATCA AGGAAACTCA CAGCCTCAGC AAAAGTATAC TGTGATGTCA
    GGAACACCTG AAAAAATTTT AGAGCATTTT CTAGAAACAA TACGCCTTGA GGCAACTTTA
    AATGAAGCAA CAGATTCTGT TTTAAATGAC TTTATTATGA TGCACTGTGT TTTTATGCCA
    AATACCCAGC TTTGCCCGGC ACTGGTGGCC CACTACCACG CACAGCCTTC ACAAGGTACA
    GAACAGGAGA AAATGGATTA TGCCCTCAAC AATAAGAGGC GAGTCATCCG CCTGGTTCTA
    CAGTGGGCTG CCATGTATGG AGACCTCCTG CAAGAGGATG ACGTGTCTAT GGCCTTCCTG
    GAGGAGTTTT ATGTATCTGT ATCAGATGAT GCCCGGATGA TTGCTGCCCT CAAGGAGCAA
    CTGCCAGAGT TGGAGAAGAT TGTCAAGCAA ATCTCAGAAG ATGCAAAGGC ACCACAAAAG
    AAGCACAAGG TTCTTTTGCA ACAGTTCAAT ACGGGCGATG AGAGAGCCCA GAAGCGCCAG
    CCTATCCGCG GCTCTGATGA AGTTCTGTTT AAGGTCTATT GCATGGACCA CACCTACACA
    ACCATTCGGG TGCCAGTGGC CACTTCGGTG AAGGAAGTCA TCAGTGCAGT TGCCGACAAG
    CTGGGCTCCG GGGAGGGCCT GATCATAGTC AAGATGAGTT CCGGAGGAGA AAAGGTGGTG
    CTCAAACCTA ATGATGTTTC AGTATTTACG ACGCTCACCA TTAATGGACG CCTGTTTGCT
    TGCCCGCGAG AGCAATTCGA TTCACTGACT CCCTTACCAG AACAGGAAGG CCCAACTGTT
    GGAACAGTGG GAACTTTTGA ACTGATGAGC TCCAAAGATT TAGCATACCA GATGACAATT
    TATGATTGGG AACTCTTCAA CTGCGTGCAT GAGCTGGAGC TAATCTATCA CACATTTGGA
    AGGCATAATT TTAAAAAGAC CACAGCAAAC TTGGATTTGT TCCTGAGGAG ATTTAATGAA
    ATTCAGTTTT GGGTCGTCAC TGAGATCTGC CTTTGTTCTC AGCTCAGCAA GCGTGTTCAG
    CTATTAAAAA AATTTATTAA GATAGCAGCC CACTGTAAGG AGTATAAAAA TCTGAATTCC
    TTTTTTGCCA TCGTCATGGG ACTAAGTAAC GTTGCTGTGA GCCGCTTGGC ACTAACGTGG
    GAGAAACTGC CAAGCAAGTT CAAGAAGTTC TATGCGGAGT TTGAAAGTTT AATGGACCCT
    TCAAGGAACC ACAGGGCCTA CAGGCTGACA GTAGCTAAGC TGGAACCTCC TCTCATCCCC
    TTCATGCCTT TGCTCATTAA AGATATGACA TTTACTCATG AGGGGAACAA GACGTTCATT
    GACAATCTAG TAAACTTTGA AAAAATGCGC ATGATTGCAA ATACGGCCAG AACAGTGAGA
    TACTACAGGA GCCAACCCTT CAATCCTGAT GCAGCTCAAG CTAATAAGAA CCATCAGGAT
    GTCCGGAGTT ATGTACGGCA ATTAAATGTG ATTGACAACC AGAGAACTTT ATCACAGATG
    TCACACAGAT TAGAGCCTCG TCGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858686.1
    CDS190..2739
    Translation

    Target ORF information:

    RefSeq Version XM_017003197.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTCTACA AGAAATATCG ACAGTATATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACGTCCC TTCAGAGAAG
    ATCCTCAGAG CTGGAAAAAT TTTACGAAAT GCCATTCTCT CTCGAGCACC TCACATGATA
    AGAGATAGAA AATACCACCT AAAGACATAC AGACAATGCT GTGTGGGAAC TGAACTGGTG
    GACTGGATGA TGCAGCAGAC ACCATGTGTT CACTCCCGGA CTCAAGCTGT TGGCATGTGG
    CAAGTCCTGT TAGAAGATGG TGTTCTCAAC CACGTGGACC AGGAGCACCA TTTCCAAGAC
    AAATATTTAT TCTATCGATT TCTGGATGAT GAGCACGAGG ATGCCCCTTT GCCTACTGAG
    GAGGAGAAGA AGGAGTGTGA TGAGGAGCTC CAGGACACCA TGCTGCTGCT GTCACAGATG
    GGCCCCGACG CCCACATGAG GATGATCCTT CGCAAACCAC CTGGCCAGAG GACTGTGGAT
    GACCTAGAGA TTATCTATGA GGAGCTTCTT CATATTAAAG CCTTATCCCA TCTTTCTACC
    ACAGTGAAAC GAGAGTTAGC AGGTGTTCTC ATTTTTGAGT CTCACGCCAA AGGAGGGACT
    GTGTTGTTTA ACCAGGGGGA AGAAGGTACC TCCTGGTACA TTATTCTAAA AGGATCAGTG
    AATGTAGTCA TTTACGGCAA GGGTGTGGTC TGCACCCTGC ATGAAGGAGA TGACTTCGGC
    AAGTTAGCAC TAGTGAATGA TGCCCCACGA GCTGCCTCTA TCGTCTTACG AGAAGATAAC
    TGCCATTTCT TAAGAGTAGA CAAGGAGGAT TTCAACCGGA TCCTAAGGGA CGTGGAGGCG
    AATACAGTCA GACTTAAAGA ACATGACCAA GATGTCTTGG TGCTGGAGAA GGTCCCAGCA
    GGGAACAGAG CTTCTAATCA AGGAAACTCA CAGCCTCAGC AAAAGTATAC TGTGATGTCA
    GGAACACCTG AAAAAATTTT AGAGCATTTT CTAGAAACAA TACGCCTTGA GGCAACTTTA
    AATGAAGCAA CAGATTCTGT TTTAAATGAC TTTATTATGA TGCACTGTGT TTTTATGCCA
    AATACCCAGC TTTGCCCGGC ACTGGTGGCC CACTACCACG CACAGCCTTC ACAAGGTACA
    GAACAGGAGA AAATGGATTA TGCCCTCAAC AATAAGAGGC GAGTCATCCG CCTGGTTCTA
    CAGTGGGCTG CCATGTATGG AGACCTCCTG CAAGAGGATG ACGTGTCTAT GGCCTTCCTG
    GAGGAGTTTT ATGTATCTGT ATCAGATGAT GCCCGGATGA TTGCTGCCCT CAAGGAGCAA
    CTGCCAGAGT TGGAGAAGAT TGTCAAGCAA ATCTCAGAAG ATGCAAAGGC ACCACAAAAG
    AAGCACAAGG TTCTTTTGCA ACAGTTCAAT ACGGGCGATG AGAGAGCCCA GAAGCGCCAG
    CCTATCCGCG GCTCTGATGA AGTTCTGTTT AAGGTCTATT GCATGGACCA CACCTACACA
    ACCATTCGGG TGCCAGTGGC CACTTCGGTG AAGGAAGTCA TCAGTGCAGT TGCCGACAAG
    CTGGGCTCCG GGGAGGGCCT GATCATAGTC AAGATGAGTT CCGGAGGAGA AAAGGTGGTG
    CTCAAACCTA ATGATGTTTC AGTATTTACG ACGCTCACCA TTAATGGACG CCTGTTTGCT
    TGCCCGCGAG AGCAATTCGA TTCACTGACT CCCTTACCAG AACAGGAAGG CCCAACTGTT
    GGAACAGTGG GAACTTTTGA ACTGATGAGC TCCAAAGATT TAGCATACCA GATGACAATT
    TATGATTGGG AACTCTTCAA CTGCGTGCAT GAGCTGGAGC TAATCTATCA CACATTTGGA
    AGGCATAATT TTAAAAAGAC CACAGCAAAC TTGGATTTGT TCCTGAGGAG ATTTAATGAA
    ATTCAGTTTT GGGTCGTCAC TGAGATCTGC CTTTGTTCTC AGCTCAGCAA GCGTGTTCAG
    CTATTAAAAA AATTTATTAA GATAGCAGCC CACTGTAAGG AGTATAAAAA TCTGAATTCC
    TTTTTTGCCA TCGTCATGGG ACTAAGTAAC GTTGCTGTGA GCCGCTTGGC ACTAACGTGG
    GAGAAACTGC CAAGCAAGTT CAAGAAGTTC TATGCGGAGT TTGAAAGTTT AATGGACCCT
    TCAAGGAACC ACAGGGCCTA CAGGCTGACA GTAGCTAAGC TGGAACCTCC TCTCATCCCC
    TTCATGCCTT TGCTCATTAA AGATATGACA TTTACTCATG AGGGGAACAA GACGTTCATT
    GACAATCTAG TAAACTTTGA AAAAATGCGC ATGATTGCAA ATACGGCCAG AACAGTGAGA
    TACTACAGGA GCCAACCCTT CAATCCTGAT GCAGCTCAAG CTAATAAGAA CCATCAGGAT
    GTCCGGAGTT ATGTACGGCA ATTAAATGTG ATTGACAACC AGAGAACTTT ATCACAGATG
    TCACACAGAT TAGAGCCTCG TCGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109026
    Clone ID Related Accession (Same CDS sequence) NM_001375864.1
    Accession Version NM_001375864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform f
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.46521.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCATACCC TTTCCGGCTA TTCAGACCTT GCGGGACCAC TGGAGCGATC CAGCGAAGAT 
    GTGGATATAA TCTTCACTCG ACTGAAAGAA GTTAAAGCTT TTGAGAAATT TCACCCAAAT
    CTCCTTCATC AGATTTGCTT ATGTGGTTAT TATGAGAATC TGGAAAAGGG AATAACATTA
    TTTCGCCAGG GTGATATTGG AACAAACTGG TATGCTGTCC TGGCAGGGTC TTTGGATGTT
    AAAGTATCTG AGACCAGCAG TCACCAGGAT GCTGTGACCA TCTGTACCCT GGGAATTGGG
    ACGGCCTTTG GAGAGTCCAT TCTGGACAAC ACACCCCGCC ATGCAACCAT CGTTACCAGG
    GAGAGCAGTG AACTGCTCCG CATCGAGCAG AAGGACTTCA AGGCACTATG GGAGAAATAT
    CGACAGTATA TGGCAGGACT TCTGGCTCCT CCTTATGGTG TTATGGAAAC GGGCTCTAAC
    AATGACAGGA TTCCTGACAA GGAGAACACA CCTCTCATTG AACCTCACGT TCCTCTTCGT
    CCTGCTAACA CCATTACCAA GGTCCCTTCA GAGAAGATCC TCAGAGCTGG AAAAATTTTA
    CGAAATGCCA TTCTCTCTCG AGCACCTCAC ATGATAAGAG ATAGAAAATA CCACCTAAAG
    ACATACAGAC AATGCTGTGT GGGAACTGAA CTGGTGGACT GGATGATGCA GCAGACACCA
    TGTGTTCACT CCCGGACTCA AGCTGTTGGC ATGTGGCAAG TCCTGTTAGA AGATGGTGTT
    CTCAACCACG TGGACCAGGA GCACCATTTC CAAGACAAAT ATTTATTCTA TCGATTTCTG
    GATGATGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGACGCCCA CATGAGGATG
    ATCCTTCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAGATTAT CTATGAGGAG
    CTTCTTCATA TTAAAGCCTT ATCCCATCTT TCTACCACAG TGAAACGAGA GTTAGCAGGT
    GTTCTCATTT TTGAGTCTCA CGCCAAAGGA GGGACTGTGT TGTTTAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCAGTGAATG TAGTCATTTA CGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA AGGAGATGAC TTCGGCAAGT TAGCACTAGT GAATGATGCC
    CCACGAGCTG CCTCTATCGT CTTACGAGAA GATAACTGCC ATTTCTTAAG AGTAGACAAG
    GAGGATTTCA ACCGGATCCT AAGGGACGTG GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGAGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGGCA ACTTTAAATG AAGCAACAGA TTCTGTTTTA
    AATGACTTTA TTATGATGCA CTGTGTTTTT ATGCCAAATA CCCAGCTTTG CCCGGCACTG
    GTGGCCCACT ACCACGCACA GCCTTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGGCGAGT CATCCGCCTG GTTCTACAGT GGGCTGCCAT GTATGGAGAC
    CTCCTGCAAG AGGATGACGT GTCTATGGCC TTCCTGGAGG AGTTTTATGT ATCTGTATCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CAGAGTTGGA GAAGATTGTC
    AAGCAAATCT CAGAAGATGC AAAGGCACCA CAAAAGAAGC ACAAGGTTCT TTTGCAACAG
    TTCAATACGG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGCGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC AGTGGCCACT
    TCGGTGAAGG AAGTCATCAG TGCAGTTGCC GACAAGCTGG GCTCCGGGGA GGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACGACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGATTCA
    CTGACTCCCT TACCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGC
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGGAGATTT AATGAAATTC AGTTTTGGGT CGTCACTGAG
    ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT GTTCAGCTAT TAAAAAAATT TATTAAGATA
    GCAGCCCACT GTAAGGAGTA TAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAACGTTG CTGTGAGCCG CTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGTTTAATG GACCCTTCAA GGAACCACAG GGCCTACAGG
    CTGACAGTAG CTAAGCTGGA ACCTCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACG TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC GGCCAGAACA GTGAGATACT ACAGGAGCCA ACCCTTCAAT
    CCTGATGCAG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362793.1
    CDS547..3552
    Translation

    Target ORF information:

    RefSeq Version NM_001375864.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCATACCC TTTCCGGCTA TTCAGACCTT GCGGGACCAC TGGAGCGATC CAGCGAAGAT 
    GTGGATATAA TCTTCACTCG ACTGAAAGAA GTTAAAGCTT TTGAGAAATT TCACCCAAAT
    CTCCTTCATC AGATTTGCTT ATGTGGTTAT TATGAGAATC TGGAAAAGGG AATAACATTA
    TTTCGCCAGG GTGATATTGG AACAAACTGG TATGCTGTCC TGGCAGGGTC TTTGGATGTT
    AAAGTATCTG AGACCAGCAG TCACCAGGAT GCTGTGACCA TCTGTACCCT GGGAATTGGG
    ACGGCCTTTG GAGAGTCCAT TCTGGACAAC ACACCCCGCC ATGCAACCAT CGTTACCAGG
    GAGAGCAGTG AACTGCTCCG CATCGAGCAG AAGGACTTCA AGGCACTATG GGAGAAATAT
    CGACAGTATA TGGCAGGACT TCTGGCTCCT CCTTATGGTG TTATGGAAAC GGGCTCTAAC
    AATGACAGGA TTCCTGACAA GGAGAACACA CCTCTCATTG AACCTCACGT TCCTCTTCGT
    CCTGCTAACA CCATTACCAA GGTCCCTTCA GAGAAGATCC TCAGAGCTGG AAAAATTTTA
    CGAAATGCCA TTCTCTCTCG AGCACCTCAC ATGATAAGAG ATAGAAAATA CCACCTAAAG
    ACATACAGAC AATGCTGTGT GGGAACTGAA CTGGTGGACT GGATGATGCA GCAGACACCA
    TGTGTTCACT CCCGGACTCA AGCTGTTGGC ATGTGGCAAG TCCTGTTAGA AGATGGTGTT
    CTCAACCACG TGGACCAGGA GCACCATTTC CAAGACAAAT ATTTATTCTA TCGATTTCTG
    GATGATGAGC ACGAGGATGC CCCTTTGCCT ACTGAGGAGG AGAAGAAGGA GTGTGATGAG
    GAGCTCCAGG ACACCATGCT GCTGCTGTCA CAGATGGGCC CCGACGCCCA CATGAGGATG
    ATCCTTCGCA AACCACCTGG CCAGAGGACT GTGGATGACC TAGAGATTAT CTATGAGGAG
    CTTCTTCATA TTAAAGCCTT ATCCCATCTT TCTACCACAG TGAAACGAGA GTTAGCAGGT
    GTTCTCATTT TTGAGTCTCA CGCCAAAGGA GGGACTGTGT TGTTTAACCA GGGGGAAGAA
    GGTACCTCCT GGTACATTAT TCTAAAAGGA TCAGTGAATG TAGTCATTTA CGGCAAGGGT
    GTGGTCTGCA CCCTGCATGA AGGAGATGAC TTCGGCAAGT TAGCACTAGT GAATGATGCC
    CCACGAGCTG CCTCTATCGT CTTACGAGAA GATAACTGCC ATTTCTTAAG AGTAGACAAG
    GAGGATTTCA ACCGGATCCT AAGGGACGTG GAGGCGAATA CAGTCAGACT TAAAGAACAT
    GACCAAGATG TCTTGGTGCT GGAGAAGGTC CCAGCAGGGA ACAGAGCTTC TAATCAAGGA
    AACTCACAGC CTCAGCAAAA GTATACTGTG ATGTCAGGAA CACCTGAAAA AATTTTAGAG
    CATTTTCTAG AAACAATACG CCTTGAGGCA ACTTTAAATG AAGCAACAGA TTCTGTTTTA
    AATGACTTTA TTATGATGCA CTGTGTTTTT ATGCCAAATA CCCAGCTTTG CCCGGCACTG
    GTGGCCCACT ACCACGCACA GCCTTCACAA GGTACAGAAC AGGAGAAAAT GGATTATGCC
    CTCAACAATA AGAGGCGAGT CATCCGCCTG GTTCTACAGT GGGCTGCCAT GTATGGAGAC
    CTCCTGCAAG AGGATGACGT GTCTATGGCC TTCCTGGAGG AGTTTTATGT ATCTGTATCA
    GATGATGCCC GGATGATTGC TGCCCTCAAG GAGCAACTGC CAGAGTTGGA GAAGATTGTC
    AAGCAAATCT CAGAAGATGC AAAGGCACCA CAAAAGAAGC ACAAGGTTCT TTTGCAACAG
    TTCAATACGG GCGATGAGAG AGCCCAGAAG CGCCAGCCTA TCCGCGGCTC TGATGAAGTT
    CTGTTTAAGG TCTATTGCAT GGACCACACC TACACAACCA TTCGGGTGCC AGTGGCCACT
    TCGGTGAAGG AAGTCATCAG TGCAGTTGCC GACAAGCTGG GCTCCGGGGA GGGCCTGATC
    ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG GTGGTGCTCA AACCTAATGA TGTTTCAGTA
    TTTACGACGC TCACCATTAA TGGACGCCTG TTTGCTTGCC CGCGAGAGCA ATTCGATTCA
    CTGACTCCCT TACCAGAACA GGAAGGCCCA ACTGTTGGAA CAGTGGGAAC TTTTGAACTG
    ATGAGCTCCA AAGATTTAGC ATACCAGATG ACAATTTATG ATTGGGAACT CTTCAACTGC
    GTGCATGAGC TGGAGCTAAT CTATCACACA TTTGGAAGGC ATAATTTTAA AAAGACCACA
    GCAAACTTGG ATTTGTTCCT GAGGAGATTT AATGAAATTC AGTTTTGGGT CGTCACTGAG
    ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT GTTCAGCTAT TAAAAAAATT TATTAAGATA
    GCAGCCCACT GTAAGGAGTA TAAAAATCTG AATTCCTTTT TTGCCATCGT CATGGGACTA
    AGTAACGTTG CTGTGAGCCG CTTGGCACTA ACGTGGGAGA AACTGCCAAG CAAGTTCAAG
    AAGTTCTATG CGGAGTTTGA AAGTTTAATG GACCCTTCAA GGAACCACAG GGCCTACAGG
    CTGACAGTAG CTAAGCTGGA ACCTCCTCTC ATCCCCTTCA TGCCTTTGCT CATTAAAGAT
    ATGACATTTA CTCATGAGGG GAACAAGACG TTCATTGACA ATCTAGTAAA CTTTGAAAAA
    ATGCGCATGA TTGCAAATAC GGCCAGAACA GTGAGATACT ACAGGAGCCA ACCCTTCAAT
    CCTGATGCAG CTCAAGCTAA TAAGAACCAT CAGGATGTCC GGAGTTATGT ACGGCAATTA
    AATGTGATTG ACAACCAGAG AACTTTATCA CAGATGTCAC ACAGATTAGA GCCTCGTCGA
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109031
    Clone ID Related Accession (Same CDS sequence) NM_001375865.1
    Accession Version NM_001375865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform g
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.283579.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362794.1
    CDS140..3142
    Translation

    Target ORF information:

    RefSeq Version NM_001375865.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGTATTT
    ACGACGCTCA CCATTAATGG ACGCCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTAC CAGAACAGGA AGGCCCAACT GTTGGAACAG TGGGAACTTT TGAACTGATG
    AGCTCCAAAG ATTTAGCATA CCAGATGACA ATTTATGATT GGGAACTCTT CAACTGCGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATA ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGATTTAAT GAAATTCAGT TTTGGGTCGT CACTGAGATC
    TGCCTTTGTT CTCAGCTCAG CAAGCGTGTT CAGCTATTAA AAAAATTTAT TAAGATAGCA
    GCCCACTGTA AGGAGTATAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AACGTTGCTG TGAGCCGCTT GGCACTAACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCGG AGTTTGAAAG TTTAATGGAC CCTTCAAGGA ACCACAGGGC CTACAGGCTG
    ACAGTAGCTA AGCTGGAACC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TAGTAAACTT TGAAAAAATG
    CGCATGATTG CAAATACGGC CAGAACAGTG AGATACTACA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCATCAG GATGTCCGGA GTTATGTACG GCAATTAAAT
    GTGATTGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GATTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17245
    Clone ID Related Accession (Same CDS sequence) NM_007023.3 , NM_007023.4
    Accession Version NM_007023.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB471672.1, BI546558.1, AB027471.1, BC024004.2 and BQ184791.1. On Aug 10, 2007 this sequence version replaced NM_007023.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.261752.1, SRR1660805.245272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008954.2
    CDS188..3223
    Translation

    Target ORF information:

    RefSeq Version NM_007023.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007023.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17245
    Clone ID Related Accession (Same CDS sequence) NM_007023.3 , NM_007023.4
    Accession Version NM_007023.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 4 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018712.5, AC009484.3, AC104086.6 and AC019046.4. On Nov 23, 2018 this sequence version replaced NM_007023.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.237474.1, SRR1803613.166907.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397081.8/ ENSP00000380271.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008954.2
    CDS140..3175
    Translation

    Target ORF information:

    RefSeq Version NM_007023.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007023.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCTGCCG AGTGGATCGC CTGCCTGGAT 
    AAAAGACCAC TGGAGCGATC CAGCGAAGAT GTGGATATAA TCTTCACTCG ACTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGCTT ATGTGGTTAT
    TATGAGAATC TGGAAAAGGG AATAACATTA TTTCGCCAGG GTGATATTGG AACAAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTATCTG AGACCAGCAG TCACCAGGAT
    GCTGTGACCA TCTGTACCCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACACCCCGCC ATGCAACCAT CGTTACCAGG GAGAGCAGTG AACTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTATA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTCCCTTCA
    GAGAAGATCC TCAGAGCTGG AAAAATTTTA CGAAATGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT GGGAACTGAA
    CTGGTGGACT GGATGATGCA GCAGACACCA TGTGTTCACT CCCGGACTCA AGCTGTTGGC
    ATGTGGCAAG TCCTGTTAGA AGATGGTGTT CTCAACCACG TGGACCAGGA GCACCATTTC
    CAAGACAAAT ATTTATTCTA TCGATTTCTG GATGATGAGC ACGAGGATGC CCCTTTGCCT
    ACTGAGGAGG AGAAGAAGGA GTGTGATGAG GAGCTCCAGG ACACCATGCT GCTGCTGTCA
    CAGATGGGCC CCGACGCCCA CATGAGGATG ATCCTTCGCA AACCACCTGG CCAGAGGACT
    GTGGATGACC TAGAGATTAT CTATGAGGAG CTTCTTCATA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGAGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA
    GGGACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA CGGCAAGGGT GTGGTCTGCA CCCTGCATGA AGGAGATGAC
    TTCGGCAAGT TAGCACTAGT GAATGATGCC CCACGAGCTG CCTCTATCGT CTTACGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT AAGGGACGTG
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGGCA
    ACTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTA TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCGGCACTG GTGGCCCACT ACCACGCACA GCCTTCACAA
    GGTACAGAAC AGGAGAAAAT GGATTATGCC CTCAACAATA AGAGGCGAGT CATCCGCCTG
    GTTCTACAGT GGGCTGCCAT GTATGGAGAC CTCCTGCAAG AGGATGACGT GTCTATGGCC
    TTCCTGGAGG AGTTTTATGT ATCTGTATCA GATGATGCCC GGATGATTGC TGCCCTCAAG
    GAGCAACTGC CAGAGTTGGA GAAGATTGTC AAGCAAATCT CAGAAGATGC AAAGGCACCA
    CAAAAGAAGC ACAAGGTTCT TTTGCAACAG TTCAATACGG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGCGGCTC TGATGAAGTT CTGTTTAAGG TCTATTGCAT GGACCACACC
    TACACAACCA TTCGGGTGCC AGTGGCCACT TCGGTGAAGG AAGTCATCAG TGCAGTTGCC
    GACAAGCTGG GCTCCGGGGA GGGCCTGATC ATAGTCAAGA TGAGTTCCGG AGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TACCAGAACA GGAAGGCCCA
    ACTGTTGGAA CAGTGGGAAC TTTTGAACTG ATGAGCTCCA AAGATTTAGC ATACCAGATG
    ACAATTTATG ATTGGGAACT CTTCAACTGC GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT
    AATGAAATTC AGTTTTGGGT CGTCACTGAG ATCTGCCTTT GTTCTCAGCT CAGCAAGCGT
    GTTCAGCTAT TAAAAAAATT TATTAAGATA GCAGCCCACT GTAAGGAGTA TAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAACGTTG CTGTGAGCCG CTTGGCACTA
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CGGAGTTTGA AAGTTTAATG
    GACCCTTCAA GGAACCACAG GGCCTACAGG CTGACAGTAG CTAAGCTGGA ACCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC GGCCAGAACA
    GTGAGATACT ACAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAT
    CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGATTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.