CHAT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHAT
Entrez Gene ID	1103
Full Name	choline O-acetyltransferase
Synonyms	CHOACTASE,CMS1A,CMS1A2,CMS6
General protein information	Preferred Names choline O-acetyltransferase Names choline O-acetyltransferase acetyl CoA:choline O-acetyltransferase choline acetylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q11.23
Summary	This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010].
Disorder MIM:	118490
Disorder Html:	Myasthenic syndrome, congenital, 6, presynaptic, 254210 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_020984.3	NP_066264.3	choline O-acetyltransferase isoform 1
NM_020549.4	NP_065574.3	choline O-acetyltransferase isoform 2
NM_020986.3	NP_066266.3	choline O-acetyltransferase isoform 1
NM_001142929.1	NP_001136401.1	choline O-acetyltransferase isoform 1
NM_001142933.1	NP_001136405.1	choline O-acetyltransferase isoform 3
NM_001142934.1	NP_001136406.1	choline O-acetyltransferase isoform 1
NM_020985.3	NP_066265.3	choline O-acetyltransferase isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chat	NP_034021.1
Drosophila melanogaster (fruit fly)	Cha	NP_477004.5
Homo sapiens (human)	CHAT	NP_001136405.1
Pan troglodytes (chimpanzee)	CHAT	XP_003339420.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002370	XP_312586.5
Caenorhabditis elegans (roundworm)	cha-1	NP_001023603.1
Xenopus tropicalis (tropical clawed frog)	chat	XP_002933959.1
	CHAT	XP_002805679.1
Bos taurus (cattle)	CHAT	XP_002698999.2
Canis lupus familiaris (dog)	CHAT	XP_005637542.1
Rattus norvegicus (Norway rat)	Chat	NP_001164064.1
Gallus gallus (chicken)	CHAT	NP_989941.1
Danio rerio (zebrafish)	chat	NP_001124191.1

Gene Ontology
Bibliography

Related articles in PubMed

The Choline Acetyltransferase (CHAT) Gene is Associated with Parahippocampal and Hippocampal Structure and Short-term Memory Span.
Zhu B, Chen C, Moyzis RK, Dong Q, Lin C
Neuroscience369261-268(2018 Jan)

Human choline acetyltransferase mRNAs with different 5'-region produce a 69-kDa major translation product.
Misawa H, Matsuura J, Oda Y, Takahashi R, Deguchi T
Brain research. Molecular brain research44(2)323-33(1997 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Compared with the control group, the densitometric quantification and mean density of GPR43 and ChAT proteins, and expression of GPR43 and CHAT genes, were significantly decreased in the patients with mixed refractory constipation.
Title: Function and clinical implications of short-chain fatty acids in patients with mixed refractory constipation.

We conducted a meta-analysis of studies involving CHAT, TFAM, and VR22 polymorphisms and Alzheimer disease susceptibility.For CHAT, rs2177369 (G>A) in whites and rs3810950 (G>A) in Asians were found to be associated with AD susceptibility. No association was detected between rs1880676 and rs868750 and AD risk.
Title: Polymorphisms of CHAT but not TFAM or VR22 are Associated with Alzheimer Disease Risk.

Results suggest that SATB1 is activated to bind to chromatin at S/MARs after exposure to Abeta 1-42, resulting in alternative utilization and movement of 82-kDa ChAT to these regions demonstrating that both proteins play critical roles in the response of neural cells to acute Abeta-exposure.
Title: 82-kDa choline acetyltransferase and SATB1 localize to ?-amyloid induced matrix attachment regions.

In conclusion, our meta-analysis indicated CHAT rs2177369 polymorphism might play a protective role in AD, while rs3810950 variant was a risk factor for AD but its single heterozygous mutations might not influence susceptibility to AD.
Title: Genetic Association of CHAT rs3810950 and rs2177369 Polymorphisms with the Risk of Alzheimer's Disease: A Meta-Analysis.

meta-analysis suggested that rs1880670G/A, and rs2177369 G/A polymorphisms were not risk factors for Alzheimer's Disease. However, rs3810950G/A, or rs868750G/A genetic polymorphism was a genetic risk factor for the development of Alzheimer's Disease.
Title: Association of Choline Acetyltransferase Gene Polymorphisms (SNPs rs868750G/A, rs1880676G/A, rs2177369G/A and rs3810950G/A) with Alzheimer's Disease Risk: A Meta-Analysis.

The following CHAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16386	NM_001142934.1 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu16386	NM_020985.3 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu16386	NM_020984.3 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu16386	NM_001142929.1 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu16386	NM_020986.3 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu16430	NM_001142933.1 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$468.30 $669.00
OHu19162	NM_020549.4 Latest version!	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHAT ( NM_020984.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066264.3 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA.

Price & Availability↑

CloneID	OHu16386
Clone ID Related Accession (Same CDS sequence)	NM_020984.3 , NM_020986.3 , NM_001142929.1 , NM_001142934.1 , NM_020985.3
Accession Version	NM_020984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305909.1 and AC073366.4. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced NM_020984.2. Transcript Variant: This variant (R) contains alternate 5' exon R (PMID 7616604) and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305909.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066264.3
CDS	174..2066
Translation	MAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_020984.3
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020984.3

ORF Insert Sequence:

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( NM_020986.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066266.3 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA.

Price & Availability↑

CloneID	OHu16386
Clone ID Related Accession (Same CDS sequence)	NM_020984.3 , NM_020986.3 , NM_001142929.1 , NM_001142934.1 , NM_020985.3
Accession Version	NM_020986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D82340.1, D82341.1, AC073366.4, BC130615.1 and AF305907.1. On May 21, 2008 this sequence version replaced NM_020986.2. Transcript Variant: This variant (N2) contains alternate 5' exon N and encodes isoform 1 (PMID 9073174). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF305909.1, BC130615.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066266.3
CDS	164..2056
Translation	MAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_020986.3
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020986.3

ORF Insert Sequence:

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( NM_001142929.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136401.1 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

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CloneID	OHu16386
Clone ID Related Accession (Same CDS sequence)	NM_020984.3 , NM_020986.3 , NM_001142929.1 , NM_001142934.1 , NM_020985.3
Accession Version	NM_001142929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073366.4 and AF305907.1. Transcript Variant: This variant (M) contains alternate 5' exon M (PMID 7616604) but uses a downstream start codon and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305907.1, S56138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1337937) RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136401.1
CDS	508..2400
Translation	MAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_001142929.1
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142929.1

ORF Insert Sequence:

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( NM_001142934.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136406.1 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

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CloneID	OHu16386
Clone ID Related Accession (Same CDS sequence)	NM_020984.3 , NM_020986.3 , NM_001142929.1 , NM_001142934.1 , NM_020985.3
Accession Version	NM_001142934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305908.2 and AC073366.4. Transcript Variant: This variant (S) contains alternate 5' exons M and S in the 5' coding region but uses a downstream start codon and encodes isoform 1 (PMID 11172068). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305908.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1337937) ##RefSeq-Attributes-END##

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136406.1
CDS	578..2470
Translation	MAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_001142934.1
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142934.1

ORF Insert Sequence:

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( NM_020985.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066265.3 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N1, mRNA.

Price & Availability↑

CloneID	OHu16386
Clone ID Related Accession (Same CDS sequence)	NM_020984.3 , NM_020986.3 , NM_001142929.1 , NM_001142934.1 , NM_020985.3
Accession Version	NM_020985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D82340.1, AC073366.4, BC130615.1 and AF305907.1. On May 21, 2008 this sequence version replaced NM_020985.2. Transcript Variant: This variant (N1) contains alternate 5' exon N and uses an alternate splice site within alternate exon M, and encodes isoform 1 (PMID 9073174). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF305909.1, BC130615.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 5' end.

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066265.3
CDS	336..2228
Translation	MAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_020985.3
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020985.3

ORF Insert Sequence:

ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG 
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC 
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG 
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG 
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC 
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT 
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC 
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG 
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC 
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA 
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG 
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG 
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC 
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC 
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG 
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC 
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC 
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG 
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC 
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC 
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC 
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG 
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG 
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG 
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG 
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC 
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT 
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC 
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG 
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA 
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( NM_001142933.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136405.1 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

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CloneID	OHu16430
Clone ID Related Accession (Same CDS sequence)	NM_001142933.1
Accession Version	NM_001142933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305908.2 and AC073366.4. Transcript Variant: This variant (S) contains alternate 5' exons M and S in the 5' coding region, and uses an upstream start codon (PMID 11172068). The resulting protein (isoform 3) has a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305908.2 [ECO:0000332] ##Evidence-Data-END##

ATGTGGCCGG AATGCAGAGA TGAAGCACTG AGCACAGTAG GTCCACACCT CTGCATCCCT 
GCACCAGGAC TCACCAAGAC GCCCATCCTG GAAAAGGTCC CCCGTAAGAT GGCAGCAAAA 
ACTCCCAGCA GTGAGGAGTC TGGGCTGCCC AAACTGCCCG TGCCCCCGCT GCAGCAGACC 
CTGGCCACGT ACCTGCAGTG CATGCGACAC TTGGTGTCTG AGGAGCAGTT CAGGAAGAGC 
CAGGCCATTG TGCAGCAGTT TGGGGCCCCT GGTGGCCTCG GCGAGACCCT GCAGCAGAAA 
CTCCTGGAGC GGCAGGAGAA GACAGCCAAC TGGGTGTCTG AGTACTGGCT GAATGACATG 
TATCTCAACA ACCGCCTGGC CCTGCCTGTC AACTCCAGCC CTGCCGTGAT CTTTGCTCGG 
CAGCACTTCC CTGGCACCGA TGACCAGCTG AGGTTTGCAG CCAGCCTCAT CTCTGGTGTA 
CTCAGCTACA AGGCCCTGCT GGACAGCCAC TCCATTCCCA CTGACTGTGC CAAAGGCCAG 
CTGTCAGGGC AGCCCCTTTG CATGAAGCAA TACTATGGGC TCTTCTCCTC CTACCGGCTC 
CCCGGCCATA CCCAGGACAC GCTGGTGGCT CAGAACAGCA GCATCATGCC GGAGCCTGAG 
CACGTCATCG TAGCCTGCTG CAATCAGTTC TTTGTCTTGG ATGTTGTCAT TAATTTCCGC 
CGTCTCAGTG AGGGGGATCT GTTCACTCAG TTGAGAAAGA TAGTCAAAAT GGCTTCCAAC 
GAGGACGAGC GTTTGCCTCC AATTGGCCTG CTGACGTCTG ACGGGAGGAG CGAGTGGGCC 
GAGGCCAGGA CGGTCCTCGT GAAAGACTCC ACCAACCGGG ACTCGCTGGA CATGATTGAG 
CGCTGCATCT GCCTTGTATG CCTGGACGCG CCAGGAGGCG TGGAGCTCAG CGACACCCAC 
AGGGCACTCC AGCTCCTTCA CGGCGGAGGC TACAGCAAGA ACGGGGCCAA TCGCTGGTAC 
GACAAGTCCC TGCAGTTTGT GGTGGGCCGA GACGGCACCT GCGGTGTGGT GTGCGAACAC 
TCCCCATTCG ATGGCATCGT CCTGGTGCAG TGCACTGAGC ATCTGCTCAA GCACATGACG 
CAGAGCAGCA GGAAGCTGAT CCGAGCAGAC TCCGTCAGCG AGCTCCCCGC CCCCCGGAGG 
CTGCGGTGGA AATGCTCCCC GGAAATTCAA GGCCACTTAG CCTCCTCGGC AGAAAAACTT 
CAACGAATAG TAAAGAACCT TGACTTCATT GTCTATAAGT TTGACAACTA TGGGAAAACA 
TTCATTAAGA AGCAGAAATG CAGCCCTGAT GCCTTCATCC AGGTGGCCCT CCAGCTGGCC 
TTCTACAGGC TCCATCGAAG ACTGGTGCCC ACCTACGAGA GCGCGTCCAT CCGCCGATTC 
CAGGAGGGAC GCGTGGACAA CATCAGATCG GCCACTCCAG AGGCACTGGC TTTTGTGAGA 
GCCGTGACTG ACCACAAGGC TGCTGTGCCA GCTTCTGAGA AGCTTCTGCT CCTGAAGGAT 
GCCATCCGTG CCCAGACTGC ATACACAGTC ATGGCCATAA CAGGGATGGC CATTGACAAC 
CACCTGCTGG CACTGCGGGA GCTGGCCCGG GCCATGTGCA AGGAGCTGCC CGAGATGTTC 
ATGGATGAAA CCTACCTGAT GAGCAACCGG TTTGTCCTCT CCACTAGCCA GGTGCCCACA 
ACCACGGAGA TGTTCTGCTG CTATGGTCCT GTGGTCCCAA ATGGGTATGG TGCCTGCTAC 
AACCCCCAGC CAGAGACCAT CCTTTTCTGC ATCTCTAGCT TTCACAGCTG CAAAGAGACT 
TCTTCTAGCA AGTTTGCAAA AGCTGTGGAA GAAAGCCTCA TTGACATGAG AGACCTCTGC 
AGTCTGCTGC CGCCTACTGA GAGCAAGCCA TTGGCAACAA AGGAAAAAGC CACGAGGCCC 
AGCCAGGGAC ACCAACCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136405.1
CDS	470..2470
Translation	MWPECRDEALSTVGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_001142933.1
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142933.1

ORF Insert Sequence:

ATGTGGCCGG AATGCAGAGA TGAAGCACTG AGCACAGTAG GTCCACACCT CTGCATCCCT 
GCACCAGGAC TCACCAAGAC GCCCATCCTG GAAAAGGTCC CCCGTAAGAT GGCAGCAAAA 
ACTCCCAGCA GTGAGGAGTC TGGGCTGCCC AAACTGCCCG TGCCCCCGCT GCAGCAGACC 
CTGGCCACGT ACCTGCAGTG CATGCGACAC TTGGTGTCTG AGGAGCAGTT CAGGAAGAGC 
CAGGCCATTG TGCAGCAGTT TGGGGCCCCT GGTGGCCTCG GCGAGACCCT GCAGCAGAAA 
CTCCTGGAGC GGCAGGAGAA GACAGCCAAC TGGGTGTCTG AGTACTGGCT GAATGACATG 
TATCTCAACA ACCGCCTGGC CCTGCCTGTC AACTCCAGCC CTGCCGTGAT CTTTGCTCGG 
CAGCACTTCC CTGGCACCGA TGACCAGCTG AGGTTTGCAG CCAGCCTCAT CTCTGGTGTA 
CTCAGCTACA AGGCCCTGCT GGACAGCCAC TCCATTCCCA CTGACTGTGC CAAAGGCCAG 
CTGTCAGGGC AGCCCCTTTG CATGAAGCAA TACTATGGGC TCTTCTCCTC CTACCGGCTC 
CCCGGCCATA CCCAGGACAC GCTGGTGGCT CAGAACAGCA GCATCATGCC GGAGCCTGAG 
CACGTCATCG TAGCCTGCTG CAATCAGTTC TTTGTCTTGG ATGTTGTCAT TAATTTCCGC 
CGTCTCAGTG AGGGGGATCT GTTCACTCAG TTGAGAAAGA TAGTCAAAAT GGCTTCCAAC 
GAGGACGAGC GTTTGCCTCC AATTGGCCTG CTGACGTCTG ACGGGAGGAG CGAGTGGGCC 
GAGGCCAGGA CGGTCCTCGT GAAAGACTCC ACCAACCGGG ACTCGCTGGA CATGATTGAG 
CGCTGCATCT GCCTTGTATG CCTGGACGCG CCAGGAGGCG TGGAGCTCAG CGACACCCAC 
AGGGCACTCC AGCTCCTTCA CGGCGGAGGC TACAGCAAGA ACGGGGCCAA TCGCTGGTAC 
GACAAGTCCC TGCAGTTTGT GGTGGGCCGA GACGGCACCT GCGGTGTGGT GTGCGAACAC 
TCCCCATTCG ATGGCATCGT CCTGGTGCAG TGCACTGAGC ATCTGCTCAA GCACATGACG 
CAGAGCAGCA GGAAGCTGAT CCGAGCAGAC TCCGTCAGCG AGCTCCCCGC CCCCCGGAGG 
CTGCGGTGGA AATGCTCCCC GGAAATTCAA GGCCACTTAG CCTCCTCGGC AGAAAAACTT 
CAACGAATAG TAAAGAACCT TGACTTCATT GTCTATAAGT TTGACAACTA TGGGAAAACA 
TTCATTAAGA AGCAGAAATG CAGCCCTGAT GCCTTCATCC AGGTGGCCCT CCAGCTGGCC 
TTCTACAGGC TCCATCGAAG ACTGGTGCCC ACCTACGAGA GCGCGTCCAT CCGCCGATTC 
CAGGAGGGAC GCGTGGACAA CATCAGATCG GCCACTCCAG AGGCACTGGC TTTTGTGAGA 
GCCGTGACTG ACCACAAGGC TGCTGTGCCA GCTTCTGAGA AGCTTCTGCT CCTGAAGGAT 
GCCATCCGTG CCCAGACTGC ATACACAGTC ATGGCCATAA CAGGGATGGC CATTGACAAC 
CACCTGCTGG CACTGCGGGA GCTGGCCCGG GCCATGTGCA AGGAGCTGCC CGAGATGTTC 
ATGGATGAAA CCTACCTGAT GAGCAACCGG TTTGTCCTCT CCACTAGCCA GGTGCCCACA 
ACCACGGAGA TGTTCTGCTG CTATGGTCCT GTGGTCCCAA ATGGGTATGG TGCCTGCTAC 
AACCCCCAGC CAGAGACCAT CCTTTTCTGC ATCTCTAGCT TTCACAGCTG CAAAGAGACT 
TCTTCTAGCA AGTTTGCAAA AGCTGTGGAA GAAAGCCTCA TTGACATGAG AGACCTCTGC 
AGTCTGCTGC CGCCTACTGA GAGCAAGCCA TTGGCAACAA AGGAAAAAGC CACGAGGCCC 
AGCCAGGGAC ACCAACCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( NM_020549.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065574.3 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

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CloneID	OHu19162
Clone ID Related Accession (Same CDS sequence)	NM_020549.4
Accession Version	NM_020549.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	choline O-acetyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073366.4 and AF305907.1. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced NM_020549.3. Transcript Variant: This variant (M) contains alternate 5' exon M (PMID 7616604) and uses an upstream start codon. The resulting protein (isoform 2) has a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305907.1, S56138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGGGGCTGA GGACAGCGAA GAAGAGGGGG CTTGGGGGAG GGGGGAAATG GAAGAGAGAG 
GAGGGAGGAG GTACAAGAGG AAGGAGAGAA GTGCGGCCAG CTTGCTTTCT CCAGTCGGGT 
GGCCGCGGGG ACCCGGGCGA CGTCGGAGGC CCTGCCGGGA ACCCAGGCTG CAGCCCCCAC 
CCCCGCGCTG CGACACGCCC CCCACCCCTT CCGGCTCACA CCCCCGCCCA CACTCCTGAG 
TGGTGCGGTG CAGCGTCGGC CGAGGCAGCA GAGCCGAGGA GAGCAGGTCC ACACCTCTGC 
ATCCCTGCAC CAGGACTCAC CAAGACGCCC ATCCTGGAAA AGGTCCCCCG TAAGATGGCA 
GCAAAAACTC CCAGCAGTGA GGAGTCTGGG CTGCCCAAAC TGCCCGTGCC CCCGCTGCAG 
CAGACCCTGG CCACGTACCT GCAGTGCATG CGACACTTGG TGTCTGAGGA GCAGTTCAGG 
AAGAGCCAGG CCATTGTGCA GCAGTTTGGG GCCCCTGGTG GCCTCGGCGA GACCCTGCAG 
CAGAAACTCC TGGAGCGGCA GGAGAAGACA GCCAACTGGG TGTCTGAGTA CTGGCTGAAT 
GACATGTATC TCAACAACCG CCTGGCCCTG CCTGTCAACT CCAGCCCTGC CGTGATCTTT 
GCTCGGCAGC ACTTCCCTGG CACCGATGAC CAGCTGAGGT TTGCAGCCAG CCTCATCTCT 
GGTGTACTCA GCTACAAGGC CCTGCTGGAC AGCCACTCCA TTCCCACTGA CTGTGCCAAA 
GGCCAGCTGT CAGGGCAGCC CCTTTGCATG AAGCAATACT ATGGGCTCTT CTCCTCCTAC 
CGGCTCCCCG GCCATACCCA GGACACGCTG GTGGCTCAGA ACAGCAGCAT CATGCCGGAG 
CCTGAGCACG TCATCGTAGC CTGCTGCAAT CAGTTCTTTG TCTTGGATGT TGTCATTAAT 
TTCCGCCGTC TCAGTGAGGG GGATCTGTTC ACTCAGTTGA GAAAGATAGT CAAAATGGCT 
TCCAACGAGG ACGAGCGTTT GCCTCCAATT GGCCTGCTGA CGTCTGACGG GAGGAGCGAG 
TGGGCCGAGG CCAGGACGGT CCTCGTGAAA GACTCCACCA ACCGGGACTC GCTGGACATG 
ATTGAGCGCT GCATCTGCCT TGTATGCCTG GACGCGCCAG GAGGCGTGGA GCTCAGCGAC 
ACCCACAGGG CACTCCAGCT CCTTCACGGC GGAGGCTACA GCAAGAACGG GGCCAATCGC 
TGGTACGACA AGTCCCTGCA GTTTGTGGTG GGCCGAGACG GCACCTGCGG TGTGGTGTGC 
GAACACTCCC CATTCGATGG CATCGTCCTG GTGCAGTGCA CTGAGCATCT GCTCAAGCAC 
ATGACGCAGA GCAGCAGGAA GCTGATCCGA GCAGACTCCG TCAGCGAGCT CCCCGCCCCC 
CGGAGGCTGC GGTGGAAATG CTCCCCGGAA ATTCAAGGCC ACTTAGCCTC CTCGGCAGAA 
AAACTTCAAC GAATAGTAAA GAACCTTGAC TTCATTGTCT ATAAGTTTGA CAACTATGGG 
AAAACATTCA TTAAGAAGCA GAAATGCAGC CCTGATGCCT TCATCCAGGT GGCCCTCCAG 
CTGGCCTTCT ACAGGCTCCA TCGAAGACTG GTGCCCACCT ACGAGAGCGC GTCCATCCGC 
CGATTCCAGG AGGGACGCGT GGACAACATC AGATCGGCCA CTCCAGAGGC ACTGGCTTTT 
GTGAGAGCCG TGACTGACCA CAAGGCTGCT GTGCCAGCTT CTGAGAAGCT TCTGCTCCTG 
AAGGATGCCA TCCGTGCCCA GACTGCATAC ACAGTCATGG CCATAACAGG GATGGCCATT 
GACAACCACC TGCTGGCACT GCGGGAGCTG GCCCGGGCCA TGTGCAAGGA GCTGCCCGAG 
ATGTTCATGG ATGAAACCTA CCTGATGAGC AACCGGTTTG TCCTCTCCAC TAGCCAGGTG 
CCCACAACCA CGGAGATGTT CTGCTGCTAT GGTCCTGTGG TCCCAAATGG GTATGGTGCC 
TGCTACAACC CCCAGCCAGA GACCATCCTT TTCTGCATCT CTAGCTTTCA CAGCTGCAAA 
GAGACTTCTT CTAGCAAGTT TGCAAAAGCT GTGGAAGAAA GCCTCATTGA CATGAGAGAC 
CTCTGCAGTC TGCTGCCGCC TACTGAGAGC AAGCCATTGG CAACAAAGGA AAAAGCCACG 
AGGCCCAGCC AGGGACACCA ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065574.3
CDS	154..2400
Translation	MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPLPAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Target ORF information:

RefSeq Version	NM_020549.4
Organism	Homo sapiens(human)
Definition	Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020549.4

ORF Insert Sequence:

ATGGGGCTGA GGACAGCGAA GAAGAGGGGG CTTGGGGGAG GGGGGAAATG GAAGAGAGAG 
GAGGGAGGAG GTACAAGAGG AAGGAGAGAA GTGCGGCCAG CTTGCTTTCT CCAGTCGGGT 
GGCCGCGGGG ACCCGGGCGA CGTCGGAGGC CCTGCCGGGA ACCCAGGCTG CAGCCCCCAC 
CCCCGCGCTG CGACACGCCC CCCACCCCTT CCGGCTCACA CCCCCGCCCA CACTCCTGAG 
TGGTGCGGTG CAGCGTCGGC CGAGGCAGCA GAGCCGAGGA GAGCAGGTCC ACACCTCTGC 
ATCCCTGCAC CAGGACTCAC CAAGACGCCC ATCCTGGAAA AGGTCCCCCG TAAGATGGCA 
GCAAAAACTC CCAGCAGTGA GGAGTCTGGG CTGCCCAAAC TGCCCGTGCC CCCGCTGCAG 
CAGACCCTGG CCACGTACCT GCAGTGCATG CGACACTTGG TGTCTGAGGA GCAGTTCAGG 
AAGAGCCAGG CCATTGTGCA GCAGTTTGGG GCCCCTGGTG GCCTCGGCGA GACCCTGCAG 
CAGAAACTCC TGGAGCGGCA GGAGAAGACA GCCAACTGGG TGTCTGAGTA CTGGCTGAAT 
GACATGTATC TCAACAACCG CCTGGCCCTG CCTGTCAACT CCAGCCCTGC CGTGATCTTT 
GCTCGGCAGC ACTTCCCTGG CACCGATGAC CAGCTGAGGT TTGCAGCCAG CCTCATCTCT 
GGTGTACTCA GCTACAAGGC CCTGCTGGAC AGCCACTCCA TTCCCACTGA CTGTGCCAAA 
GGCCAGCTGT CAGGGCAGCC CCTTTGCATG AAGCAATACT ATGGGCTCTT CTCCTCCTAC 
CGGCTCCCCG GCCATACCCA GGACACGCTG GTGGCTCAGA ACAGCAGCAT CATGCCGGAG 
CCTGAGCACG TCATCGTAGC CTGCTGCAAT CAGTTCTTTG TCTTGGATGT TGTCATTAAT 
TTCCGCCGTC TCAGTGAGGG GGATCTGTTC ACTCAGTTGA GAAAGATAGT CAAAATGGCT 
TCCAACGAGG ACGAGCGTTT GCCTCCAATT GGCCTGCTGA CGTCTGACGG GAGGAGCGAG 
TGGGCCGAGG CCAGGACGGT CCTCGTGAAA GACTCCACCA ACCGGGACTC GCTGGACATG 
ATTGAGCGCT GCATCTGCCT TGTATGCCTG GACGCGCCAG GAGGCGTGGA GCTCAGCGAC 
ACCCACAGGG CACTCCAGCT CCTTCACGGC GGAGGCTACA GCAAGAACGG GGCCAATCGC 
TGGTACGACA AGTCCCTGCA GTTTGTGGTG GGCCGAGACG GCACCTGCGG TGTGGTGTGC 
GAACACTCCC CATTCGATGG CATCGTCCTG GTGCAGTGCA CTGAGCATCT GCTCAAGCAC 
ATGACGCAGA GCAGCAGGAA GCTGATCCGA GCAGACTCCG TCAGCGAGCT CCCCGCCCCC 
CGGAGGCTGC GGTGGAAATG CTCCCCGGAA ATTCAAGGCC ACTTAGCCTC CTCGGCAGAA 
AAACTTCAAC GAATAGTAAA GAACCTTGAC TTCATTGTCT ATAAGTTTGA CAACTATGGG 
AAAACATTCA TTAAGAAGCA GAAATGCAGC CCTGATGCCT TCATCCAGGT GGCCCTCCAG 
CTGGCCTTCT ACAGGCTCCA TCGAAGACTG GTGCCCACCT ACGAGAGCGC GTCCATCCGC 
CGATTCCAGG AGGGACGCGT GGACAACATC AGATCGGCCA CTCCAGAGGC ACTGGCTTTT 
GTGAGAGCCG TGACTGACCA CAAGGCTGCT GTGCCAGCTT CTGAGAAGCT TCTGCTCCTG 
AAGGATGCCA TCCGTGCCCA GACTGCATAC ACAGTCATGG CCATAACAGG GATGGCCATT 
GACAACCACC TGCTGGCACT GCGGGAGCTG GCCCGGGCCA TGTGCAAGGA GCTGCCCGAG 
ATGTTCATGG ATGAAACCTA CCTGATGAGC AACCGGTTTG TCCTCTCCAC TAGCCAGGTG 
CCCACAACCA CGGAGATGTT CTGCTGCTAT GGTCCTGTGG TCCCAAATGG GTATGGTGCC 
TGCTACAACC CCCAGCCAGA GACCATCCTT TTCTGCATCT CTAGCTTTCA CAGCTGCAAA 
GAGACTTCTT CTAGCAAGTT TGCAAAAGCT GTGGAAGAAA GCCTCATTGA CATGAGAGAC 
CTCTGCAGTC TGCTGCCGCC TACTGAGAGC AAGCCATTGG CAACAAAGGA AAAAGCCACG 
AGGCCCAGCC AGGGACACCA ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Choline Acetyltransferase (CHAT) Gene is Associated with Parahippocampal and Hippocampal Structure and Short-term Memory Span.
Neuroscience369261-268(2018 Jan)
Zhu B,Chen C,Moyzis RK,Dong Q,Lin C

Human choline acetyltransferase mRNAs with different 5'-region produce a 69-kDa major translation product.
Brain research. Molecular brain research44(2)323-33(1997 Mar)
Misawa H,Matsuura J,Oda Y,Takahashi R,Deguchi T

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CHAT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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