PNPLA6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNPLA6
Entrez Gene ID	10908
Full Name	patatin like phospholipase domain containing 6
Synonyms	BNHS,LNMS,NTE,NTEMND,OMCS,SPG39,iPLA2delta,sws
General protein information	Preferred Names patatin like phospholipase domain containing 6 Names neuropathy target esterase patatin-like phospholipase domain-containing protein 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	This gene encodes a phospholipase that deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine. It is thought to function in neurite outgrowth and process elongation during neuronal differentiation. The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells. Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009].
Disorder MIM:	603197
Disorder Html:	Spastic paraplegia 39, autosomal recessive, 612020 (3); Boucher-Neuhauser syndrome, 215470 (3); ?Laurence-Moon syndrome, 245800 (3); Oliver-McFarlane syndrome, 275400 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006702.5	NP_006693.3	neuropathy target esterase isoform b
NM_001166114.1	NP_001159586.1	neuropathy target esterase isoform d
NM_001166111.2	NP_001159583.1	neuropathy target esterase isoform a
NM_001166111.1	NP_001159583.1	neuropathy target esterase isoform a
NM_001166114.2	NP_001159586.1	neuropathy target esterase isoform d
NM_001166112.1	NP_001159584.1	neuropathy target esterase isoform c
NM_006702.4	NP_006693.3	neuropathy target esterase isoform b
NM_001166113.1	NP_001159585.1	neuropathy target esterase isoform b

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PNPLA6	NP_001070015.2
Homo sapiens (human)	PNPLA6	NP_001159583.1
Danio rerio (zebrafish)	LOC560986	XP_005160109.1
Canis lupus familiaris (dog)	PNPLA6	XP_005632988.1
Mus musculus (house mouse)	Pnpla6	NP_001116290.2
Rattus norvegicus (Norway rat)	Pnpla6	XP_341026.6
Gallus gallus (chicken)	PNPLA6	NP_001106924.1
Pan troglodytes (chimpanzee)	PNPLA6	XP_512331.3

Gene Ontology
Bibliography

Related articles in PubMed

Boucher Neuh?user Syndrome - A rare cause of inherited hypogonadotropic hypogonadism. A case of two adult siblings with two novel mutations in PNPLA6.
Langdahl JH, Frederiksen AL, Nguyen N, Brusgaard K, Juhl CB
European journal of medical genetics60(2)105-109(2017 Feb)

The PNPLA-family phospholipases involved in glycerophospholipid homeostasis of HeLa cells.
Hermansson M, H?nninen S, Hokynar K, Somerharju P
Biochimica et biophysica acta1861(9 Pt A)1058-1065(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The unusual medical history with childhood ataxia and hypogonadotropic hypogonadism lead to further examinations and eventually the diagnosis of BNS. The older sister of the proband also displayed the triad of ataxia, HH and chorioretinal dystrophy accompanied by cerebellar atrophy and in 2014, we found the mutations in PNPLA6.
Title: Boucher Neuh?user Syndrome - A rare cause of inherited hypogonadotropic hypogonadism. A case of two adult siblings with two novel mutations in PNPLA6.

These results strongly suggest that PNPLA9, -6 and -4 play a key role in GPL turnover and homeostasis in human cells. A hypothetical model suggesting how these enzymes could recognize the relative concentration of the different GPLs is proposed
Title: The PNPLA-family phospholipases involved in glycerophospholipid homeostasis of HeLa cells.

Data indicate two novel homozygous mutations (one frameshift and one missense mutation) detected in CYP7B1 (SPG5A), while no disease-causing mutation was identified for PNPLA6 (SPG39) and C19orf12 (SPG43).
Title: Mutational analysis of the CYP7B1, PNPLA6 and C19orf12 genes in autosomal recessive hereditary spastic paraplegia.

we found a novel compound heterozygous mutation and a novel homozygous one in the PNPLA6 gene in two Japanese patients with BNS.
Title: Novel mutations in the PNPLA6 gene in Boucher-Neuh?user syndrome.

inducible, neuron-specific expression of full-length human wildtype NTE reduces vacuole formation and substantially rescues mobility.
Title: Delayed Induction of Human NTE (PNPLA6) Rescues Neurodegeneration and Mobility Defects of Drosophila swiss cheese (sws) Mutants.

The following PNPLA6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNPLA6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12003	NM_001166113.1 Latest version!	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu12003	NM_006702.5 Latest version!	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu10887	NM_001166112.1 Latest version!	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$916.30 $1309.00
OHu11758	NM_001166114.2 Latest version!	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu12332	NM_001166111.2 Latest version!	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu12332	NM_001166111.1	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu11758	NM_001166114.1	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu12003	NM_006702.4	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNPLA6 ( NM_006702.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006693.3 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12003
Clone ID Related Accession (Same CDS sequence)	NM_006702.5 , NM_006702.4 , NM_001166113.1
Accession Version	NM_006702.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA104284.1, BC050553.1 and BC051768.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_006702.4. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment near the N-terminus, compared to isoform a. Both variants 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACCCCT CCCTCCTGAA CAGCAGAGTC 
TTGCTGCACC ACGCCAAAGC TGGCACCATC ATTGCCCGCC AGGGAGACCA GGACGTGAGC 
CTGCACTTCG TGCTCTGGGG CTGCCTGCAC GTGTACCAGC GCATGATCGA CAAGGCGGAG 
GACGTGTGCC TGTTCGTAGC GCAGCCCGGG GAACTGGTGG GGCAGCTGGC GGTGCTCACT 
GGCGAACCTC TCATCTTCAC ACTGCGAGCC CAACGCGACT GCACCTTCCT GCGGATCTCC 
AAGTCCGACT TCTATGAGAT CATGCGCGCA CAGCCCAGTG TGGTGCTGAG TGCGGCGCAC 
ACGGTGGCAG CCAGGATGTC GCCCTTCGTG CGCCAGATGG ACTTCGCCAT CGACTGGACT 
GCAGTGGAGG CGGGACGCGC GCTGTACAGG CAGGGCGACC GCTCCGACTG CACTTACATC 
GTGCTCAATG GGCGGCTGCG TAGCGTGATC CAGCGAGGCA GTGGCAAGAA GGAGCTGGTG 
GGCGAGTACG GCCGCGGCGA CCTCATCGGC GTGGTGGAGG CACTGACCCG GCAGCCGCGA 
GCCACGACGG TGCACGCGGT GCGCGACACG GAGCTGGCCA AGCTTCCCGA GGGCACCTTG 
GGTCACATCA AACGCCGGTA CCCGCAGGTC GTGACCCGCC TTATCCACCT ACTGAGCCAG 
AAAATTCTAG GGAATTTGCA GCAGCTGCAA GGACCCTTCC CAGCAGGCTC TGGGTTGGGT 
GTGCCCCCAC ACTCGGAACT CACCAACCCA GCCAGCAACC TGGCAACTGT GGCAATCCTG 
CCTGTGTGTG CTGAGGTCCC CATGGTGGCC TTCACGCTGG AGCTGCAGCA CGCCCTGCAG 
GCCATCGGTC CGACGCTACT CCTTAACAGT GACATCATCC GGGCACGCCT GGGGGCCTCC 
GCACTGGATA GCATCCAAGA GTTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGATGCA 
CACCGTATCG TACTCTACCA GACGGACGCC TCGCTGACGC CCTGGACCGT GCGCTGCCTG 
CGACAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAGCCTAC CCTCGGCCAG 
CTGGAGCAGA TGCTGGAGAA CACGGCTGTG CGCGCCCTTA AGCAGCTAGT CCTGCTCCAC 
CGAGAGGAGG GCGCGGGCCC CACGCGCACC GTGGAGTGGC TAAATATGCG CAGCTGGTGC 
TCGGGGCACC TGCACCTGCG CTGTCCGCGC CGCCTCTTTT CGCGCCGCAG CCCTGCCAAG 
CTGCATGAGC TCTACGAGAA GGTTTTCTCC AGGCGCGCGG ACCGGCACAG CGACTTCTCC 
CGCTTGGCGA GGGTGCTCAC GGGGAACACC ATTGCCCTTG TGCTAGGCGG GGGCGGGGCC 
AGGGGCTGCT CGCACATCGG AGTACTAAAG GCATTAGAGG AGGCGGGGGT CCCCGTGGAC 
CTGGTGGGCG GCACGTCCAT TGGCTCTTTC ATCGGAGCGT TGTACGCGGA GGAGCGCAGC 
GCCAGCCGCA CGAAGCAGCG GGCCCGGGAG TGGGCCAAGA GCATGACTTC GGTGCTGGAA 
CCTGTGTTGG ACCTCACGTA CCCAGTCACC TCCATGTTCA CTGGGTCTGC CTTTAACCGC 
AGCATCCATC GGGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAC 
GTGACCACAG ATATCACCGC CTCAGCCATG CGAGTCCACA AAGATGGCTC CCTGTGGCGG 
TACGTGCGCG CCAGCATGAC GCTGTCGGGC TACCTGCCCC CGCTGTGCGA CCCCAAGGAC 
GGGCACCTAC TCATGGATGG CGGCTACATC AACAATCTGC CAGCGGACAT CGCCCGCAGC 
ATGGGTGCCA AAACGGTCAT CGCCATTGAC GTGGGGAGCC AGGATGAGAC GGACCTCAGC 
ACCTACGGGG ACAGCCTGTC CGGCTGGTGG CTGCTGTGGA AGCGGCTGAA TCCCTGGGCT 
GACAAGGTAA AGGTTCCAGA CATGGCTGAA ATCCAGTCCC GCCTGGCCTA CGTGTCCTGT 
GTGCGGCAGC TAGAGGTTGT CAAGTCCAGC TCCTACTGCG AGTACCTGCG CCCGCCCATC 
GACTGCTTCA AGACCATGGA CTTTGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG 
TACGGGAAGG CGGTGTTTGG AGGCTGGAGC CGTGGCAACG TCATTGAGAA AATGCTCACA 
GACCGGCGGT CTACAGACCT TAATGAGAGC CGCCGTGCAG ACGTGCTTGC CTTCCCAAGC 
TCTGGCTTCA CTGACTTGGC AGAGATTGTG TCCCGGATTG AGCCCCCCAC GAGCTATGTC 
TCTGATGGCT GTGCTGACGG AGAGGAGTCA GATTGTCTGA CAGAGTATGA GGAGGACGCC 
GGACCCGACT GCTCGAGGGA TGAAGGGGGG TCCCCCGAGG GCGCAAGCCC CAGCACTGCC 
TCCGAGATGG AGGAGGAGAA GTCGATTCTC CGGCAACGAC GCTGTCTGCC CCAGGAGCCG 
CCCGGCTCAG CCACAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006693.3
CDS	272..4255
Translation	MEAPLQTGMVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPAGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_006702.5
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006702.5

ORF Insert Sequence:

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACCCCT CCCTCCTGAA CAGCAGAGTC 
TTGCTGCACC ACGCCAAAGC TGGCACCATC ATTGCCCGCC AGGGAGACCA GGACGTGAGC 
CTGCACTTCG TGCTCTGGGG CTGCCTGCAC GTGTACCAGC GCATGATCGA CAAGGCGGAG 
GACGTGTGCC TGTTCGTAGC GCAGCCCGGG GAACTGGTGG GGCAGCTGGC GGTGCTCACT 
GGCGAACCTC TCATCTTCAC ACTGCGAGCC CAACGCGACT GCACCTTCCT GCGGATCTCC 
AAGTCCGACT TCTATGAGAT CATGCGCGCA CAGCCCAGTG TGGTGCTGAG TGCGGCGCAC 
ACGGTGGCAG CCAGGATGTC GCCCTTCGTG CGCCAGATGG ACTTCGCCAT CGACTGGACT 
GCAGTGGAGG CGGGACGCGC GCTGTACAGG CAGGGCGACC GCTCCGACTG CACTTACATC 
GTGCTCAATG GGCGGCTGCG TAGCGTGATC CAGCGAGGCA GTGGCAAGAA GGAGCTGGTG 
GGCGAGTACG GCCGCGGCGA CCTCATCGGC GTGGTGGAGG CACTGACCCG GCAGCCGCGA 
GCCACGACGG TGCACGCGGT GCGCGACACG GAGCTGGCCA AGCTTCCCGA GGGCACCTTG 
GGTCACATCA AACGCCGGTA CCCGCAGGTC GTGACCCGCC TTATCCACCT ACTGAGCCAG 
AAAATTCTAG GGAATTTGCA GCAGCTGCAA GGACCCTTCC CAGCAGGCTC TGGGTTGGGT 
GTGCCCCCAC ACTCGGAACT CACCAACCCA GCCAGCAACC TGGCAACTGT GGCAATCCTG 
CCTGTGTGTG CTGAGGTCCC CATGGTGGCC TTCACGCTGG AGCTGCAGCA CGCCCTGCAG 
GCCATCGGTC CGACGCTACT CCTTAACAGT GACATCATCC GGGCACGCCT GGGGGCCTCC 
GCACTGGATA GCATCCAAGA GTTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGATGCA 
CACCGTATCG TACTCTACCA GACGGACGCC TCGCTGACGC CCTGGACCGT GCGCTGCCTG 
CGACAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAGCCTAC CCTCGGCCAG 
CTGGAGCAGA TGCTGGAGAA CACGGCTGTG CGCGCCCTTA AGCAGCTAGT CCTGCTCCAC 
CGAGAGGAGG GCGCGGGCCC CACGCGCACC GTGGAGTGGC TAAATATGCG CAGCTGGTGC 
TCGGGGCACC TGCACCTGCG CTGTCCGCGC CGCCTCTTTT CGCGCCGCAG CCCTGCCAAG 
CTGCATGAGC TCTACGAGAA GGTTTTCTCC AGGCGCGCGG ACCGGCACAG CGACTTCTCC 
CGCTTGGCGA GGGTGCTCAC GGGGAACACC ATTGCCCTTG TGCTAGGCGG GGGCGGGGCC 
AGGGGCTGCT CGCACATCGG AGTACTAAAG GCATTAGAGG AGGCGGGGGT CCCCGTGGAC 
CTGGTGGGCG GCACGTCCAT TGGCTCTTTC ATCGGAGCGT TGTACGCGGA GGAGCGCAGC 
GCCAGCCGCA CGAAGCAGCG GGCCCGGGAG TGGGCCAAGA GCATGACTTC GGTGCTGGAA 
CCTGTGTTGG ACCTCACGTA CCCAGTCACC TCCATGTTCA CTGGGTCTGC CTTTAACCGC 
AGCATCCATC GGGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAC 
GTGACCACAG ATATCACCGC CTCAGCCATG CGAGTCCACA AAGATGGCTC CCTGTGGCGG 
TACGTGCGCG CCAGCATGAC GCTGTCGGGC TACCTGCCCC CGCTGTGCGA CCCCAAGGAC 
GGGCACCTAC TCATGGATGG CGGCTACATC AACAATCTGC CAGCGGACAT CGCCCGCAGC 
ATGGGTGCCA AAACGGTCAT CGCCATTGAC GTGGGGAGCC AGGATGAGAC GGACCTCAGC 
ACCTACGGGG ACAGCCTGTC CGGCTGGTGG CTGCTGTGGA AGCGGCTGAA TCCCTGGGCT 
GACAAGGTAA AGGTTCCAGA CATGGCTGAA ATCCAGTCCC GCCTGGCCTA CGTGTCCTGT 
GTGCGGCAGC TAGAGGTTGT CAAGTCCAGC TCCTACTGCG AGTACCTGCG CCCGCCCATC 
GACTGCTTCA AGACCATGGA CTTTGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG 
TACGGGAAGG CGGTGTTTGG AGGCTGGAGC CGTGGCAACG TCATTGAGAA AATGCTCACA 
GACCGGCGGT CTACAGACCT TAATGAGAGC CGCCGTGCAG ACGTGCTTGC CTTCCCAAGC 
TCTGGCTTCA CTGACTTGGC AGAGATTGTG TCCCGGATTG AGCCCCCCAC GAGCTATGTC 
TCTGATGGCT GTGCTGACGG AGAGGAGTCA GATTGTCTGA CAGAGTATGA GGAGGACGCC 
GGACCCGACT GCTCGAGGGA TGAAGGGGGG TCCCCCGAGG GCGCAAGCCC CAGCACTGCC 
TCCGAGATGG AGGAGGAGAA GTCGATTCTC CGGCAACGAC GCTGTCTGCC CCAGGAGCCG 
CCCGGCTCAG CCACAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_006702.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006693.3 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12003
Clone ID Related Accession (Same CDS sequence)	NM_006702.5 , NM_006702.4 , NM_001166113.1
Accession Version	NM_006702.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA104284.1, BC050553.1, BC051768.1 and BQ001677.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2009 this sequence version replaced NM_006702.3. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment near the N-terminus, compared to isoform a. Both variants 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACCCCT CCCTCCTGAA CAGCAGAGTC 
TTGCTGCACC ACGCCAAAGC TGGCACCATC ATTGCCCGCC AGGGAGACCA GGACGTGAGC 
CTGCACTTCG TGCTCTGGGG CTGCCTGCAC GTGTACCAGC GCATGATCGA CAAGGCGGAG 
GACGTGTGCC TGTTCGTAGC GCAGCCCGGG GAACTGGTGG GGCAGCTGGC GGTGCTCACT 
GGCGAACCTC TCATCTTCAC ACTGCGAGCC CAACGCGACT GCACCTTCCT GCGGATCTCC 
AAGTCCGACT TCTATGAGAT CATGCGCGCA CAGCCCAGTG TGGTGCTGAG TGCGGCGCAC 
ACGGTGGCAG CCAGGATGTC GCCCTTCGTG CGCCAGATGG ACTTCGCCAT CGACTGGACT 
GCAGTGGAGG CGGGACGCGC GCTGTACAGG CAGGGCGACC GCTCCGACTG CACTTACATC 
GTGCTCAATG GGCGGCTGCG TAGCGTGATC CAGCGAGGCA GTGGCAAGAA GGAGCTGGTG 
GGCGAGTACG GCCGCGGCGA CCTCATCGGC GTGGTGGAGG CACTGACCCG GCAGCCGCGA 
GCCACGACGG TGCACGCGGT GCGCGACACG GAGCTGGCCA AGCTTCCCGA GGGCACCTTG 
GGTCACATCA AACGCCGGTA CCCGCAGGTC GTGACCCGCC TTATCCACCT ACTGAGCCAG 
AAAATTCTAG GGAATTTGCA GCAGCTGCAA GGACCCTTCC CAGCAGGCTC TGGGTTGGGT 
GTGCCCCCAC ACTCGGAACT CACCAACCCA GCCAGCAACC TGGCAACTGT GGCAATCCTG 
CCTGTGTGTG CTGAGGTCCC CATGGTGGCC TTCACGCTGG AGCTGCAGCA CGCCCTGCAG 
GCCATCGGTC CGACGCTACT CCTTAACAGT GACATCATCC GGGCACGCCT GGGGGCCTCC 
GCACTGGATA GCATCCAAGA GTTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGATGCA 
CACCGTATCG TACTCTACCA GACGGACGCC TCGCTGACGC CCTGGACCGT GCGCTGCCTG 
CGACAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAGCCTAC CCTCGGCCAG 
CTGGAGCAGA TGCTGGAGAA CACGGCTGTG CGCGCCCTTA AGCAGCTAGT CCTGCTCCAC 
CGAGAGGAGG GCGCGGGCCC CACGCGCACC GTGGAGTGGC TAAATATGCG CAGCTGGTGC 
TCGGGGCACC TGCACCTGCG CTGTCCGCGC CGCCTCTTTT CGCGCCGCAG CCCTGCCAAG 
CTGCATGAGC TCTACGAGAA GGTTTTCTCC AGGCGCGCGG ACCGGCACAG CGACTTCTCC 
CGCTTGGCGA GGGTGCTCAC GGGGAACACC ATTGCCCTTG TGCTAGGCGG GGGCGGGGCC 
AGGGGCTGCT CGCACATCGG AGTACTAAAG GCATTAGAGG AGGCGGGGGT CCCCGTGGAC 
CTGGTGGGCG GCACGTCCAT TGGCTCTTTC ATCGGAGCGT TGTACGCGGA GGAGCGCAGC 
GCCAGCCGCA CGAAGCAGCG GGCCCGGGAG TGGGCCAAGA GCATGACTTC GGTGCTGGAA 
CCTGTGTTGG ACCTCACGTA CCCAGTCACC TCCATGTTCA CTGGGTCTGC CTTTAACCGC 
AGCATCCATC GGGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAC 
GTGACCACAG ATATCACCGC CTCAGCCATG CGAGTCCACA AAGATGGCTC CCTGTGGCGG 
TACGTGCGCG CCAGCATGAC GCTGTCGGGC TACCTGCCCC CGCTGTGCGA CCCCAAGGAC 
GGGCACCTAC TCATGGATGG CGGCTACATC AACAATCTGC CAGCGGACAT CGCCCGCAGC 
ATGGGTGCCA AAACGGTCAT CGCCATTGAC GTGGGGAGCC AGGATGAGAC GGACCTCAGC 
ACCTACGGGG ACAGCCTGTC CGGCTGGTGG CTGCTGTGGA AGCGGCTGAA TCCCTGGGCT 
GACAAGGTAA AGGTTCCAGA CATGGCTGAA ATCCAGTCCC GCCTGGCCTA CGTGTCCTGT 
GTGCGGCAGC TAGAGGTTGT CAAGTCCAGC TCCTACTGCG AGTACCTGCG CCCGCCCATC 
GACTGCTTCA AGACCATGGA CTTTGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG 
TACGGGAAGG CGGTGTTTGG AGGCTGGAGC CGTGGCAACG TCATTGAGAA AATGCTCACA 
GACCGGCGGT CTACAGACCT TAATGAGAGC CGCCGTGCAG ACGTGCTTGC CTTCCCAAGC 
TCTGGCTTCA CTGACTTGGC AGAGATTGTG TCCCGGATTG AGCCCCCCAC GAGCTATGTC 
TCTGATGGCT GTGCTGACGG AGAGGAGTCA GATTGTCTGA CAGAGTATGA GGAGGACGCC 
GGACCCGACT GCTCGAGGGA TGAAGGGGGG TCCCCCGAGG GCGCAAGCCC CAGCACTGCC 
TCCGAGATGG AGGAGGAGAA GTCGATTCTC CGGCAACGAC GCTGTCTGCC CCAGGAGCCG 
CCCGGCTCAG CCACAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006693.3
CDS	284..4267
Translation	MEAPLQTGMVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPAGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_006702.4
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006702.4

ORF Insert Sequence:

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACCCCT CCCTCCTGAA CAGCAGAGTC 
TTGCTGCACC ACGCCAAAGC TGGCACCATC ATTGCCCGCC AGGGAGACCA GGACGTGAGC 
CTGCACTTCG TGCTCTGGGG CTGCCTGCAC GTGTACCAGC GCATGATCGA CAAGGCGGAG 
GACGTGTGCC TGTTCGTAGC GCAGCCCGGG GAACTGGTGG GGCAGCTGGC GGTGCTCACT 
GGCGAACCTC TCATCTTCAC ACTGCGAGCC CAACGCGACT GCACCTTCCT GCGGATCTCC 
AAGTCCGACT TCTATGAGAT CATGCGCGCA CAGCCCAGTG TGGTGCTGAG TGCGGCGCAC 
ACGGTGGCAG CCAGGATGTC GCCCTTCGTG CGCCAGATGG ACTTCGCCAT CGACTGGACT 
GCAGTGGAGG CGGGACGCGC GCTGTACAGG CAGGGCGACC GCTCCGACTG CACTTACATC 
GTGCTCAATG GGCGGCTGCG TAGCGTGATC CAGCGAGGCA GTGGCAAGAA GGAGCTGGTG 
GGCGAGTACG GCCGCGGCGA CCTCATCGGC GTGGTGGAGG CACTGACCCG GCAGCCGCGA 
GCCACGACGG TGCACGCGGT GCGCGACACG GAGCTGGCCA AGCTTCCCGA GGGCACCTTG 
GGTCACATCA AACGCCGGTA CCCGCAGGTC GTGACCCGCC TTATCCACCT ACTGAGCCAG 
AAAATTCTAG GGAATTTGCA GCAGCTGCAA GGACCCTTCC CAGCAGGCTC TGGGTTGGGT 
GTGCCCCCAC ACTCGGAACT CACCAACCCA GCCAGCAACC TGGCAACTGT GGCAATCCTG 
CCTGTGTGTG CTGAGGTCCC CATGGTGGCC TTCACGCTGG AGCTGCAGCA CGCCCTGCAG 
GCCATCGGTC CGACGCTACT CCTTAACAGT GACATCATCC GGGCACGCCT GGGGGCCTCC 
GCACTGGATA GCATCCAAGA GTTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGATGCA 
CACCGTATCG TACTCTACCA GACGGACGCC TCGCTGACGC CCTGGACCGT GCGCTGCCTG 
CGACAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAGCCTAC CCTCGGCCAG 
CTGGAGCAGA TGCTGGAGAA CACGGCTGTG CGCGCCCTTA AGCAGCTAGT CCTGCTCCAC 
CGAGAGGAGG GCGCGGGCCC CACGCGCACC GTGGAGTGGC TAAATATGCG CAGCTGGTGC 
TCGGGGCACC TGCACCTGCG CTGTCCGCGC CGCCTCTTTT CGCGCCGCAG CCCTGCCAAG 
CTGCATGAGC TCTACGAGAA GGTTTTCTCC AGGCGCGCGG ACCGGCACAG CGACTTCTCC 
CGCTTGGCGA GGGTGCTCAC GGGGAACACC ATTGCCCTTG TGCTAGGCGG GGGCGGGGCC 
AGGGGCTGCT CGCACATCGG AGTACTAAAG GCATTAGAGG AGGCGGGGGT CCCCGTGGAC 
CTGGTGGGCG GCACGTCCAT TGGCTCTTTC ATCGGAGCGT TGTACGCGGA GGAGCGCAGC 
GCCAGCCGCA CGAAGCAGCG GGCCCGGGAG TGGGCCAAGA GCATGACTTC GGTGCTGGAA 
CCTGTGTTGG ACCTCACGTA CCCAGTCACC TCCATGTTCA CTGGGTCTGC CTTTAACCGC 
AGCATCCATC GGGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAC 
GTGACCACAG ATATCACCGC CTCAGCCATG CGAGTCCACA AAGATGGCTC CCTGTGGCGG 
TACGTGCGCG CCAGCATGAC GCTGTCGGGC TACCTGCCCC CGCTGTGCGA CCCCAAGGAC 
GGGCACCTAC TCATGGATGG CGGCTACATC AACAATCTGC CAGCGGACAT CGCCCGCAGC 
ATGGGTGCCA AAACGGTCAT CGCCATTGAC GTGGGGAGCC AGGATGAGAC GGACCTCAGC 
ACCTACGGGG ACAGCCTGTC CGGCTGGTGG CTGCTGTGGA AGCGGCTGAA TCCCTGGGCT 
GACAAGGTAA AGGTTCCAGA CATGGCTGAA ATCCAGTCCC GCCTGGCCTA CGTGTCCTGT 
GTGCGGCAGC TAGAGGTTGT CAAGTCCAGC TCCTACTGCG AGTACCTGCG CCCGCCCATC 
GACTGCTTCA AGACCATGGA CTTTGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG 
TACGGGAAGG CGGTGTTTGG AGGCTGGAGC CGTGGCAACG TCATTGAGAA AATGCTCACA 
GACCGGCGGT CTACAGACCT TAATGAGAGC CGCCGTGCAG ACGTGCTTGC CTTCCCAAGC 
TCTGGCTTCA CTGACTTGGC AGAGATTGTG TCCCGGATTG AGCCCCCCAC GAGCTATGTC 
TCTGATGGCT GTGCTGACGG AGAGGAGTCA GATTGTCTGA CAGAGTATGA GGAGGACGCC 
GGACCCGACT GCTCGAGGGA TGAAGGGGGG TCCCCCGAGG GCGCAAGCCC CAGCACTGCC 
TCCGAGATGG AGGAGGAGAA GTCGATTCTC CGGCAACGAC GCTGTCTGCC CCAGGAGCCG 
CCCGGCTCAG CCACAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_001166113.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159585.1 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 3, mRNA.

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CloneID	OHu12003
Clone ID Related Accession (Same CDS sequence)	NM_006702.5 , NM_006702.4 , NM_001166113.1
Accession Version	NM_001166113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC050553.1, AJ004832.1, BC051768.1 and BQ001677.1. Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The full-extent of the 5' UTR has not been determined. The resulting isoform (b) lacks an internal segment near the N-terminus, compared to isoform a. Both variants 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ004832.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACCCCT CCCTCCTGAA CAGCAGAGTC 
TTGCTGCACC ACGCCAAAGC TGGCACCATC ATTGCCCGCC AGGGAGACCA GGACGTGAGC 
CTGCACTTCG TGCTCTGGGG CTGCCTGCAC GTGTACCAGC GCATGATCGA CAAGGCGGAG 
GACGTGTGCC TGTTCGTAGC GCAGCCCGGG GAACTGGTGG GGCAGCTGGC GGTGCTCACT 
GGCGAACCTC TCATCTTCAC ACTGCGAGCC CAACGCGACT GCACCTTCCT GCGGATCTCC 
AAGTCCGACT TCTATGAGAT CATGCGCGCA CAGCCCAGTG TGGTGCTGAG TGCGGCGCAC 
ACGGTGGCAG CCAGGATGTC GCCCTTCGTG CGCCAGATGG ACTTCGCCAT CGACTGGACT 
GCAGTGGAGG CGGGACGCGC GCTGTACAGG CAGGGCGACC GCTCCGACTG CACTTACATC 
GTGCTCAATG GGCGGCTGCG TAGCGTGATC CAGCGAGGCA GTGGCAAGAA GGAGCTGGTG 
GGCGAGTACG GCCGCGGCGA CCTCATCGGC GTGGTGGAGG CACTGACCCG GCAGCCGCGA 
GCCACGACGG TGCACGCGGT GCGCGACACG GAGCTGGCCA AGCTTCCCGA GGGCACCTTG 
GGTCACATCA AACGCCGGTA CCCGCAGGTC GTGACCCGCC TTATCCACCT ACTGAGCCAG 
AAAATTCTAG GGAATTTGCA GCAGCTGCAA GGACCCTTCC CAGCAGGCTC TGGGTTGGGT 
GTGCCCCCAC ACTCGGAACT CACCAACCCA GCCAGCAACC TGGCAACTGT GGCAATCCTG 
CCTGTGTGTG CTGAGGTCCC CATGGTGGCC TTCACGCTGG AGCTGCAGCA CGCCCTGCAG 
GCCATCGGTC CGACGCTACT CCTTAACAGT GACATCATCC GGGCACGCCT GGGGGCCTCC 
GCACTGGATA GCATCCAAGA GTTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGATGCA 
CACCGTATCG TACTCTACCA GACGGACGCC TCGCTGACGC CCTGGACCGT GCGCTGCCTG 
CGACAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAGCCTAC CCTCGGCCAG 
CTGGAGCAGA TGCTGGAGAA CACGGCTGTG CGCGCCCTTA AGCAGCTAGT CCTGCTCCAC 
CGAGAGGAGG GCGCGGGCCC CACGCGCACC GTGGAGTGGC TAAATATGCG CAGCTGGTGC 
TCGGGGCACC TGCACCTGCG CTGTCCGCGC CGCCTCTTTT CGCGCCGCAG CCCTGCCAAG 
CTGCATGAGC TCTACGAGAA GGTTTTCTCC AGGCGCGCGG ACCGGCACAG CGACTTCTCC 
CGCTTGGCGA GGGTGCTCAC GGGGAACACC ATTGCCCTTG TGCTAGGCGG GGGCGGGGCC 
AGGGGCTGCT CGCACATCGG AGTACTAAAG GCATTAGAGG AGGCGGGGGT CCCCGTGGAC 
CTGGTGGGCG GCACGTCCAT TGGCTCTTTC ATCGGAGCGT TGTACGCGGA GGAGCGCAGC 
GCCAGCCGCA CGAAGCAGCG GGCCCGGGAG TGGGCCAAGA GCATGACTTC GGTGCTGGAA 
CCTGTGTTGG ACCTCACGTA CCCAGTCACC TCCATGTTCA CTGGGTCTGC CTTTAACCGC 
AGCATCCATC GGGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAC 
GTGACCACAG ATATCACCGC CTCAGCCATG CGAGTCCACA AAGATGGCTC CCTGTGGCGG 
TACGTGCGCG CCAGCATGAC GCTGTCGGGC TACCTGCCCC CGCTGTGCGA CCCCAAGGAC 
GGGCACCTAC TCATGGATGG CGGCTACATC AACAATCTGC CAGCGGACAT CGCCCGCAGC 
ATGGGTGCCA AAACGGTCAT CGCCATTGAC GTGGGGAGCC AGGATGAGAC GGACCTCAGC 
ACCTACGGGG ACAGCCTGTC CGGCTGGTGG CTGCTGTGGA AGCGGCTGAA TCCCTGGGCT 
GACAAGGTAA AGGTTCCAGA CATGGCTGAA ATCCAGTCCC GCCTGGCCTA CGTGTCCTGT 
GTGCGGCAGC TAGAGGTTGT CAAGTCCAGC TCCTACTGCG AGTACCTGCG CCCGCCCATC 
GACTGCTTCA AGACCATGGA CTTTGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG 
TACGGGAAGG CGGTGTTTGG AGGCTGGAGC CGTGGCAACG TCATTGAGAA AATGCTCACA 
GACCGGCGGT CTACAGACCT TAATGAGAGC CGCCGTGCAG ACGTGCTTGC CTTCCCAAGC 
TCTGGCTTCA CTGACTTGGC AGAGATTGTG TCCCGGATTG AGCCCCCCAC GAGCTATGTC 
TCTGATGGCT GTGCTGACGG AGAGGAGTCA GATTGTCTGA CAGAGTATGA GGAGGACGCC 
GGACCCGACT GCTCGAGGGA TGAAGGGGGG TCCCCCGAGG GCGCAAGCCC CAGCACTGCC 
TCCGAGATGG AGGAGGAGAA GTCGATTCTC CGGCAACGAC GCTGTCTGCC CCAGGAGCCG 
CCCGGCTCAG CCACAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159585.1
CDS	272..4255
Translation	MEAPLQTGMVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPAGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_001166113.1
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166113.1

ORF Insert Sequence:

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACCCCT CCCTCCTGAA CAGCAGAGTC 
TTGCTGCACC ACGCCAAAGC TGGCACCATC ATTGCCCGCC AGGGAGACCA GGACGTGAGC 
CTGCACTTCG TGCTCTGGGG CTGCCTGCAC GTGTACCAGC GCATGATCGA CAAGGCGGAG 
GACGTGTGCC TGTTCGTAGC GCAGCCCGGG GAACTGGTGG GGCAGCTGGC GGTGCTCACT 
GGCGAACCTC TCATCTTCAC ACTGCGAGCC CAACGCGACT GCACCTTCCT GCGGATCTCC 
AAGTCCGACT TCTATGAGAT CATGCGCGCA CAGCCCAGTG TGGTGCTGAG TGCGGCGCAC 
ACGGTGGCAG CCAGGATGTC GCCCTTCGTG CGCCAGATGG ACTTCGCCAT CGACTGGACT 
GCAGTGGAGG CGGGACGCGC GCTGTACAGG CAGGGCGACC GCTCCGACTG CACTTACATC 
GTGCTCAATG GGCGGCTGCG TAGCGTGATC CAGCGAGGCA GTGGCAAGAA GGAGCTGGTG 
GGCGAGTACG GCCGCGGCGA CCTCATCGGC GTGGTGGAGG CACTGACCCG GCAGCCGCGA 
GCCACGACGG TGCACGCGGT GCGCGACACG GAGCTGGCCA AGCTTCCCGA GGGCACCTTG 
GGTCACATCA AACGCCGGTA CCCGCAGGTC GTGACCCGCC TTATCCACCT ACTGAGCCAG 
AAAATTCTAG GGAATTTGCA GCAGCTGCAA GGACCCTTCC CAGCAGGCTC TGGGTTGGGT 
GTGCCCCCAC ACTCGGAACT CACCAACCCA GCCAGCAACC TGGCAACTGT GGCAATCCTG 
CCTGTGTGTG CTGAGGTCCC CATGGTGGCC TTCACGCTGG AGCTGCAGCA CGCCCTGCAG 
GCCATCGGTC CGACGCTACT CCTTAACAGT GACATCATCC GGGCACGCCT GGGGGCCTCC 
GCACTGGATA GCATCCAAGA GTTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGATGCA 
CACCGTATCG TACTCTACCA GACGGACGCC TCGCTGACGC CCTGGACCGT GCGCTGCCTG 
CGACAGGCCG ACTGCATCCT CATTGTGGGC CTGGGGGACC AGGAGCCTAC CCTCGGCCAG 
CTGGAGCAGA TGCTGGAGAA CACGGCTGTG CGCGCCCTTA AGCAGCTAGT CCTGCTCCAC 
CGAGAGGAGG GCGCGGGCCC CACGCGCACC GTGGAGTGGC TAAATATGCG CAGCTGGTGC 
TCGGGGCACC TGCACCTGCG CTGTCCGCGC CGCCTCTTTT CGCGCCGCAG CCCTGCCAAG 
CTGCATGAGC TCTACGAGAA GGTTTTCTCC AGGCGCGCGG ACCGGCACAG CGACTTCTCC 
CGCTTGGCGA GGGTGCTCAC GGGGAACACC ATTGCCCTTG TGCTAGGCGG GGGCGGGGCC 
AGGGGCTGCT CGCACATCGG AGTACTAAAG GCATTAGAGG AGGCGGGGGT CCCCGTGGAC 
CTGGTGGGCG GCACGTCCAT TGGCTCTTTC ATCGGAGCGT TGTACGCGGA GGAGCGCAGC 
GCCAGCCGCA CGAAGCAGCG GGCCCGGGAG TGGGCCAAGA GCATGACTTC GGTGCTGGAA 
CCTGTGTTGG ACCTCACGTA CCCAGTCACC TCCATGTTCA CTGGGTCTGC CTTTAACCGC 
AGCATCCATC GGGTCTTCCA GGATAAGCAG ATTGAGGACC TGTGGCTGCC TTACTTCAAC 
GTGACCACAG ATATCACCGC CTCAGCCATG CGAGTCCACA AAGATGGCTC CCTGTGGCGG 
TACGTGCGCG CCAGCATGAC GCTGTCGGGC TACCTGCCCC CGCTGTGCGA CCCCAAGGAC 
GGGCACCTAC TCATGGATGG CGGCTACATC AACAATCTGC CAGCGGACAT CGCCCGCAGC 
ATGGGTGCCA AAACGGTCAT CGCCATTGAC GTGGGGAGCC AGGATGAGAC GGACCTCAGC 
ACCTACGGGG ACAGCCTGTC CGGCTGGTGG CTGCTGTGGA AGCGGCTGAA TCCCTGGGCT 
GACAAGGTAA AGGTTCCAGA CATGGCTGAA ATCCAGTCCC GCCTGGCCTA CGTGTCCTGT 
GTGCGGCAGC TAGAGGTTGT CAAGTCCAGC TCCTACTGCG AGTACCTGCG CCCGCCCATC 
GACTGCTTCA AGACCATGGA CTTTGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG 
TACGGGAAGG CGGTGTTTGG AGGCTGGAGC CGTGGCAACG TCATTGAGAA AATGCTCACA 
GACCGGCGGT CTACAGACCT TAATGAGAGC CGCCGTGCAG ACGTGCTTGC CTTCCCAAGC 
TCTGGCTTCA CTGACTTGGC AGAGATTGTG TCCCGGATTG AGCCCCCCAC GAGCTATGTC 
TCTGATGGCT GTGCTGACGG AGAGGAGTCA GATTGTCTGA CAGAGTATGA GGAGGACGCC 
GGACCCGACT GCTCGAGGGA TGAAGGGGGG TCCCCCGAGG GCGCAAGCCC CAGCACTGCC 
TCCGAGATGG AGGAGGAGAA GTCGATTCTC CGGCAACGAC GCTGTCTGCC CCAGGAGCCG 
CCCGGCTCAG CCACAGATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_001166112.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159584.1 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 4, mRNA.

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CloneID	OHu10887
Clone ID Related Accession (Same CDS sequence)	NM_001166112.1
Accession Version	NM_001166112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302462.1, BC051768.1 and BQ001677.1. Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, uses two alternate in-frame splice sites, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (c) lacks two internal segments and one internal residue, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302462.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACGTGA GCCTGCACTT CGTGCTCTGG 
GGCTGCCTGC ACGTGTACCA GCGCATGATC GACAAGGCGG AGGACGTGTG CCTGTTCGTA 
GCGCAGCCCG GGGAACTGGT GGGGCAGCTG GCGGTGCTCA CTGGCGAACC TCTCATCTTC 
ACACTGCGAG CCCAACGCGA CTGCACCTTC CTGCGGATCT CCAAGTCCGA CTTCTATGAG 
ATCATGCGCG CACAGCCCAG TGTGGTGCTG AGTGCGGCGC ACACGGTGGC AGCCAGGATG 
TCGCCCTTCG TGCGCCAGAT GGACTTCGCC ATCGACTGGA CTGCAGTGGA GGCGGGACGC 
GCGCTGTACA GGCAGGGCGA CCGCTCCGAC TGCACTTACA TCGTGCTCAA TGGGCGGCTG 
CGTAGCGTGA TCCAGCGAGG CAGTGGCAAG AAGGAGCTGG TGGGCGAGTA CGGCCGCGGC 
GACCTCATCG GCGTGGTGGA GGCACTGACC CGGCAGCCGC GAGCCACGAC GGTGCACGCG 
GTGCGCGACA CGGAGCTGGC CAAGCTTCCC GAGGGCACCT TGGGTCACAT CAAACGCCGG 
TACCCGCAGG TCGTGACCCG CCTTATCCAC CTACTGAGCC AGAAAATTCT AGGGAATTTG 
CAGCAGCTGC AAGGACCCTT CCCAGGCTCT GGGTTGGGTG TGCCCCCACA CTCGGAACTC 
ACCAACCCAG CCAGCAACCT GGCAACTGTG GCAATCCTGC CTGTGTGTGC TGAGGTCCCC 
ATGGTGGCCT TCACGCTGGA GCTGCAGCAC GCCCTGCAGG CCATCGGTCC GACGCTACTC 
CTTAACAGTG ACATCATCCG GGCACGCCTG GGGGCCTCCG CACTGGATAG CATCCAAGAG 
TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG GAGGATGCAC ACCGTATCGT ACTCTACCAG 
ACGGACGCCT CGCTGACGCC CTGGACCGTG CGCTGCCTGC GACAGGCCGA CTGCATCCTC 
ATTGTGGGCC TGGGGGACCA GGAGCCTACC CTCGGCCAGC TGGAGCAGAT GCTGGAGAAC 
ACGGCTGTGC GCGCCCTTAA GCAGCTAGTC CTGCTCCACC GAGAGGAGGG CGCGGGCCCC 
ACGCGCACCG TGGAGTGGCT AAATATGCGC AGCTGGTGCT CGGGGCACCT GCACCTGCGC 
TGTCCGCGCC GCCTCTTTTC GCGCCGCAGC CCTGCCAAGC TGCATGAGCT CTACGAGAAG 
GTTTTCTCCA GGCGCGCGGA CCGGCACAGC GACTTCTCCC GCTTGGCGAG GGTGCTCACG 
GGGAACACCA TTGCCCTTGT GCTAGGCGGG GGCGGGGCCA GGGGCTGCTC GCACATCGGA 
GTACTAAAGG CATTAGAGGA GGCGGGGGTC CCCGTGGACC TGGTGGGCGG CACGTCCATT 
GGCTCTTTCA TCGGAGCGTT GTACGCGGAG GAGCGCAGCG CCAGCCGCAC GAAGCAGCGG 
GCCCGGGAGT GGGCCAAGAG CATGACTTCG GTGCTGGAAC CTGTGTTGGA CCTCACGTAC 
CCAGTCACCT CCATGTTCAC TGGGTCTGCC TTTAACCGCA GCATCCATCG GGTCTTCCAG 
GATAAGCAGA TTGAGGACCT GTGGCTGCCT TACTTCAACG TGACCACAGA TATCACCGCC 
TCAGCCATGC GAGTCCACAA AGATGGCTCC CTGTGGCGGT ACGTGCGCGC CAGCATGACG 
CTGTCGGGCT ACCTGCCCCC GCTGTGCGAC CCCAAGGACG GGCACCTACT CATGGATGGC 
GGCTACATCA ACAATCTGCC AGCGGACATC GCCCGCAGCA TGGGTGCCAA AACGGTCATC 
GCCATTGACG TGGGGAGCCA GGATGAGACG GACCTCAGCA CCTACGGGGA CAGCCTGTCC 
GGCTGGTGGC TGCTGTGGAA GCGGCTGAAT CCCTGGGCTG ACAAGGTAAA GGTTCCAGAC 
ATGGCTGAAA TCCAGTCCCG CCTGGCCTAC GTGTCCTGTG TGCGGCAGCT AGAGGTTGTC 
AAGTCCAGCT CCTACTGCGA GTACCTGCGC CCGCCCATCG ACTGCTTCAA GACCATGGAC 
TTTGGGAAGT TCGACCAGAT CTATGATGTG GGCTACCAGT ACGGGAAGGC GGTGTTTGGA 
GGCTGGAGCC GTGGCAACGT CATTGAGAAA ATGCTCACAG ACCGGCGGTC TACAGACCTT 
AATGAGAGCC GCCGTGCAGA CGTGCTTGCC TTCCCAAGCT CTGGCTTCAC TGACTTGGCA 
GAGATTGTGT CCCGGATTGA GCCCCCCACG AGCTATGTCT CTGATGGCTG TGCTGACGGA 
GAGGAGTCAG ATTGTCTGAC AGAGTATGAG GAGGACGCCG GACCCGACTG CTCGAGGGAT 
GAAGGGGGGT CCCCCGAGGG CGCAAGCCCC AGCACTGCCT CCGAGATGGA GGAGGAGAAG 
TCGATTCTCC GGCAACGACG CTGTCTGCCC CAGGAGCCGC CCGGCTCAGC CACAGATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159584.1
CDS	401..4303
Translation	MEAPLQTGMVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_001166112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166112.1

ORF Insert Sequence:

ATGGAGGCTC CGCTGCAAAC TGGAATGGTG CTTGGCGTGA TGATCGGGGC CGGAGTGGCG 
GTGGTGGTCA CGGCCGTGCT CATCCTCCTG GTGGTGCGGA GGCTGCGAGT GCCAAAAACC 
CCAGCCCCGG ATGGCCCCCG GTATCGGTTC CGGAAGAGGG ACAAAGTGCT CTTCTATGGC 
CGGAAGATTA TGCGGAAGGT GTCACAATCC ACCTCCTCCC TCGTGGATAC CTCTGTCTCC 
GCCACCTCCC GGCCACGCAT GAGGAAGAAA CTGAAGATGC TCAACATTGC CAAGAAGATC 
CTGCGCATCC AGAAAGAGAC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC CGCAGTGCTA 
GAAGCTGACC TGACCGAGGG CGACCTGGCT AACTCCCATC TGCCCTCTGA AGTGCTTTAT 
ATGCTCAAGA ACGTCCGGGT GCTGGGCCAC TTCGAGAAGC CACTCTTCCT GGAGCTCTGC 
CGCCACATGG TCTTCCAGCG GCTGGGCCAG GGTGACTACG TCTTCCGGCC GGGCCAGCCA 
GATGCCAGCA TCTACGTGGT GCAGGACGGG CTGCTGGAGC TCTGTCTGCC AGGGCCTGAC 
GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GCGTCAACAG CCTTCTCAGC 
ATCCTGGATG TCATCACCGG TCACCAGCAT CCCCAGCGGA CCGTGTCTGC CCGGGCGGCC 
CGGGACTCCA CGGTGCTGCG CCTGCCGGTG GAAGCATTCT CCGCGGTCTT CACCAAGTAC 
CCGGAGAGCT TGGTGCGGGT CGTGCAGATC ATCATGGTGC GGCTGCAGCG AGTCACCTTC 
CTGGCACTGC ACAACTACCT GGGTCTGACC AATGAGCTCT TCAGCCACGA GATCCAGCCC 
CTGCGTCTGT TCCCCAGCCC CGGCCTCCCA ACTCGCACCA GCCCTGTGCG GGGCTCCAAG 
AGAATGGTCA GCACCTCAGC TACAGACGAG CCCAGGGAGA CCCCAGGGCG GCCACCCGAT 
CCCACCGGGG CCCCGCTGCC TGGACCTACA GGGGACCCTG TGAAGCCCAC ATCCCTGGAA 
ACCCCCTCGG CCCCTCTGCT GAGCCGCTGC GTCTCCATGC CAGGGGACAT CTCAGGCTTG 
CAGGGTGGCC CCCGCTCCGA CTTCGACATG GCCTATGAGC GTGGCCGGAT CTCCGTGTCC 
CTGCAGGAAG AGGCCTCCGG GGGGTCCCTG GCAGCCCCCG CTCGGACCCC CACTCAGGAG 
CCTCGTGAGC AGCCGGCAGG CGCCTGTGAA TACAGCTACT GTGAGGATGA GTCGGCCACT 
GGTGGCTGCC CTTTCGGGCC CTACCAGGGC CGCCAGACCA GCAGCATCTT CGAGGCAGCA 
AAGCAGGAGC TGGCCAAGCT GATGCGGATT GAGGACGTGA GCCTGCACTT CGTGCTCTGG 
GGCTGCCTGC ACGTGTACCA GCGCATGATC GACAAGGCGG AGGACGTGTG CCTGTTCGTA 
GCGCAGCCCG GGGAACTGGT GGGGCAGCTG GCGGTGCTCA CTGGCGAACC TCTCATCTTC 
ACACTGCGAG CCCAACGCGA CTGCACCTTC CTGCGGATCT CCAAGTCCGA CTTCTATGAG 
ATCATGCGCG CACAGCCCAG TGTGGTGCTG AGTGCGGCGC ACACGGTGGC AGCCAGGATG 
TCGCCCTTCG TGCGCCAGAT GGACTTCGCC ATCGACTGGA CTGCAGTGGA GGCGGGACGC 
GCGCTGTACA GGCAGGGCGA CCGCTCCGAC TGCACTTACA TCGTGCTCAA TGGGCGGCTG 
CGTAGCGTGA TCCAGCGAGG CAGTGGCAAG AAGGAGCTGG TGGGCGAGTA CGGCCGCGGC 
GACCTCATCG GCGTGGTGGA GGCACTGACC CGGCAGCCGC GAGCCACGAC GGTGCACGCG 
GTGCGCGACA CGGAGCTGGC CAAGCTTCCC GAGGGCACCT TGGGTCACAT CAAACGCCGG 
TACCCGCAGG TCGTGACCCG CCTTATCCAC CTACTGAGCC AGAAAATTCT AGGGAATTTG 
CAGCAGCTGC AAGGACCCTT CCCAGGCTCT GGGTTGGGTG TGCCCCCACA CTCGGAACTC 
ACCAACCCAG CCAGCAACCT GGCAACTGTG GCAATCCTGC CTGTGTGTGC TGAGGTCCCC 
ATGGTGGCCT TCACGCTGGA GCTGCAGCAC GCCCTGCAGG CCATCGGTCC GACGCTACTC 
CTTAACAGTG ACATCATCCG GGCACGCCTG GGGGCCTCCG CACTGGATAG CATCCAAGAG 
TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG GAGGATGCAC ACCGTATCGT ACTCTACCAG 
ACGGACGCCT CGCTGACGCC CTGGACCGTG CGCTGCCTGC GACAGGCCGA CTGCATCCTC 
ATTGTGGGCC TGGGGGACCA GGAGCCTACC CTCGGCCAGC TGGAGCAGAT GCTGGAGAAC 
ACGGCTGTGC GCGCCCTTAA GCAGCTAGTC CTGCTCCACC GAGAGGAGGG CGCGGGCCCC 
ACGCGCACCG TGGAGTGGCT AAATATGCGC AGCTGGTGCT CGGGGCACCT GCACCTGCGC 
TGTCCGCGCC GCCTCTTTTC GCGCCGCAGC CCTGCCAAGC TGCATGAGCT CTACGAGAAG 
GTTTTCTCCA GGCGCGCGGA CCGGCACAGC GACTTCTCCC GCTTGGCGAG GGTGCTCACG 
GGGAACACCA TTGCCCTTGT GCTAGGCGGG GGCGGGGCCA GGGGCTGCTC GCACATCGGA 
GTACTAAAGG CATTAGAGGA GGCGGGGGTC CCCGTGGACC TGGTGGGCGG CACGTCCATT 
GGCTCTTTCA TCGGAGCGTT GTACGCGGAG GAGCGCAGCG CCAGCCGCAC GAAGCAGCGG 
GCCCGGGAGT GGGCCAAGAG CATGACTTCG GTGCTGGAAC CTGTGTTGGA CCTCACGTAC 
CCAGTCACCT CCATGTTCAC TGGGTCTGCC TTTAACCGCA GCATCCATCG GGTCTTCCAG 
GATAAGCAGA TTGAGGACCT GTGGCTGCCT TACTTCAACG TGACCACAGA TATCACCGCC 
TCAGCCATGC GAGTCCACAA AGATGGCTCC CTGTGGCGGT ACGTGCGCGC CAGCATGACG 
CTGTCGGGCT ACCTGCCCCC GCTGTGCGAC CCCAAGGACG GGCACCTACT CATGGATGGC 
GGCTACATCA ACAATCTGCC AGCGGACATC GCCCGCAGCA TGGGTGCCAA AACGGTCATC 
GCCATTGACG TGGGGAGCCA GGATGAGACG GACCTCAGCA CCTACGGGGA CAGCCTGTCC 
GGCTGGTGGC TGCTGTGGAA GCGGCTGAAT CCCTGGGCTG ACAAGGTAAA GGTTCCAGAC 
ATGGCTGAAA TCCAGTCCCG CCTGGCCTAC GTGTCCTGTG TGCGGCAGCT AGAGGTTGTC 
AAGTCCAGCT CCTACTGCGA GTACCTGCGC CCGCCCATCG ACTGCTTCAA GACCATGGAC 
TTTGGGAAGT TCGACCAGAT CTATGATGTG GGCTACCAGT ACGGGAAGGC GGTGTTTGGA 
GGCTGGAGCC GTGGCAACGT CATTGAGAAA ATGCTCACAG ACCGGCGGTC TACAGACCTT 
AATGAGAGCC GCCGTGCAGA CGTGCTTGCC TTCCCAAGCT CTGGCTTCAC TGACTTGGCA 
GAGATTGTGT CCCGGATTGA GCCCCCCACG AGCTATGTCT CTGATGGCTG TGCTGACGGA 
GAGGAGTCAG ATTGTCTGAC AGAGTATGAG GAGGACGCCG GACCCGACTG CTCGAGGGAT 
GAAGGGGGGT CCCCCGAGGG CGCAAGCCCC AGCACTGCCT CCGAGATGGA GGAGGAGAAG 
TCGATTCTCC GGCAACGACG CTGTCTGCCC CAGGAGCCGC CCGGCTCAGC CACAGATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_001166114.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159586.1 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu11758
Clone ID Related Accession (Same CDS sequence)	NM_001166114.1 , NM_001166114.2
Accession Version	NM_001166114.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA096881.1, BC038229.1, BC051768.1 and BQ001677.1. Transcript Variant: This variant (5) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (d) has a shorter N-terminus and lacks one internal residue, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038229.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACAT CGAGTCACGG GCTGGCTACG AACTCCTCGG GGGCGAAGGT GGCGGAGAGG 
GATGGGTTCC AGGACGTCCT GGCGCCCGGG GAAGGCTCGG CGGGACGGAT TTGCGGTGCG 
CAGCCAGTGC CGTTCGTCCC TCAGGTGCTT GGCGTGATGA TCGGGGCCGG AGTGGCGGTG 
GTGGTCACGG CCGTGCTCAT CCTCCTGGTG GTGCGGAGGC TGCGAGTGCC AAAAACCCCA 
GCCCCGGATG GCCCCCGGTA TCGGTTCCGG AAGAGGGACA AAGTGCTCTT CTATGGCCGG 
AAGATTATGC GGAAGGTGTC ACAATCCACC TCCTCCCTCG TGGATACCTC TGTCTCCGCC 
ACCTCCCGGC CACGCATGAG GAAGAAACTG AAGATGCTCA ACATTGCCAA GAAGATCCTG 
CGCATCCAGA AAGAGACGCC CACGCTGCAG CGGAAGGAGC CCCCGCCCGC AGTGCTAGAA 
GCTGACCTGA CCGAGGGCGA CCTGGCTAAC TCCCATCTGC CCTCTGAAGT GCTTTATATG 
CTCAAGAACG TCCGGGTGCT GGGCCACTTC GAGAAGCCAC TCTTCCTGGA GCTCTGCCGC 
CACATGGTCT TCCAGCGGCT GGGCCAGGGT GACTACGTCT TCCGGCCGGG CCAGCCAGAT 
GCCAGCATCT ACGTGGTGCA GGACGGGCTG CTGGAGCTCT GTCTGCCAGG GCCTGACGGG 
AAGGAGTGTG TGGTGAAGGA AGTGGTTCCT GGGGACAGCG TCAACAGCCT TCTCAGCATC 
CTGGATGTCA TCACCGGTCA CCAGCATCCC CAGCGGACCG TGTCTGCCCG GGCGGCCCGG 
GACTCCACGG TGCTGCGCCT GCCGGTGGAA GCATTCTCCG CGGTCTTCAC CAAGTACCCG 
GAGAGCTTGG TGCGGGTCGT GCAGATCATC ATGGTGCGGC TGCAGCGAGT CACCTTCCTG 
GCACTGCACA ACTACCTGGG TCTGACCAAT GAGCTCTTCA GCCACGAGAT CCAGCCCCTG 
CGTCTGTTCC CCAGCCCCGG CCTCCCAACT CGCACCAGCC CTGTGCGGGG CTCCAAGAGA 
ATGGTCAGCA CCTCAGCTAC AGACGAGCCC AGGGAGACCC CAGGGCGGCC ACCCGATCCC 
ACCGGGGCCC CGCTGCCTGG ACCTACAGGG GACCCTGTGA AGCCCACATC CCTGGAAACC 
CCCTCGGCCC CTCTGCTGAG CCGCTGCGTC TCCATGCCAG GGGACATCTC AGGCTTGCAG 
GGTGGCCCCC GCTCCGACTT CGACATGGCC TATGAGCGTG GCCGGATCTC CGTGTCCCTG 
CAGGAAGAGG CCTCCGGGGG GTCCCTGGCA GCCCCCGCTC GGACCCCCAC TCAGGAGCCT 
CGTGAGCAGC CGGCAGGCGC CTGTGAATAC AGCTACTGTG AGGATGAGTC GGCCACTGGT 
GGCTGCCCTT TCGGGCCCTA CCAGGGCCGC CAGACCAGCA GCATCTTCGA GGCAGCAAAG 
CAGGAGCTGG CCAAGCTGAT GCGGATTGAG GACCCCTCCC TCCTGAACAG CAGAGTCTTG 
CTGCACCACG CCAAAGCTGG CACCATCATT GCCCGCCAGG GAGACCAGGA CGTGAGCCTG 
CACTTCGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATCGACAA GGCGGAGGAC 
GTGTGCCTGT TCGTAGCGCA GCCCGGGGAA CTGGTGGGGC AGCTGGCGGT GCTCACTGGC 
GAACCTCTCA TCTTCACACT GCGAGCCCAA CGCGACTGCA CCTTCCTGCG GATCTCCAAG 
TCCGACTTCT ATGAGATCAT GCGCGCACAG CCCAGTGTGG TGCTGAGTGC GGCGCACACG 
GTGGCAGCCA GGATGTCGCC CTTCGTGCGC CAGATGGACT TCGCCATCGA CTGGACTGCA 
GTGGAGGCGG GACGCGCGCT GTACAGGCAG GGCGACCGCT CCGACTGCAC TTACATCGTG 
CTCAATGGGC GGCTGCGTAG CGTGATCCAG CGAGGCAGTG GCAAGAAGGA GCTGGTGGGC 
GAGTACGGCC GCGGCGACCT CATCGGCGTG GTGGAGGCAC TGACCCGGCA GCCGCGAGCC 
ACGACGGTGC ACGCGGTGCG CGACACGGAG CTGGCCAAGC TTCCCGAGGG CACCTTGGGT 
CACATCAAAC GCCGGTACCC GCAGGTCGTG ACCCGCCTTA TCCACCTACT GAGCCAGAAA 
ATTCTAGGGA ATTTGCAGCA GCTGCAAGGA CCCTTCCCAG GCTCTGGGTT GGGTGTGCCC 
CCACACTCGG AACTCACCAA CCCAGCCAGC AACCTGGCAA CTGTGGCAAT CCTGCCTGTG 
TGTGCTGAGG TCCCCATGGT GGCCTTCACG CTGGAGCTGC AGCACGCCCT GCAGGCCATC 
GGTCCGACGC TACTCCTTAA CAGTGACATC ATCCGGGCAC GCCTGGGGGC CTCCGCACTG 
GATAGCATCC AAGAGTTCCG GCTGTCAGGG TGGCTGGCCC AGCAGGAGGA TGCACACCGT 
ATCGTACTCT ACCAGACGGA CGCCTCGCTG ACGCCCTGGA CCGTGCGCTG CCTGCGACAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAGC CTACCCTCGG CCAGCTGGAG 
CAGATGCTGG AGAACACGGC TGTGCGCGCC CTTAAGCAGC TAGTCCTGCT CCACCGAGAG 
GAGGGCGCGG GCCCCACGCG CACCGTGGAG TGGCTAAATA TGCGCAGCTG GTGCTCGGGG 
CACCTGCACC TGCGCTGTCC GCGCCGCCTC TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT 
GAGCTCTACG AGAAGGTTTT CTCCAGGCGC GCGGACCGGC ACAGCGACTT CTCCCGCTTG 
GCGAGGGTGC TCACGGGGAA CACCATTGCC CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC 
TGCTCGCACA TCGGAGTACT AAAGGCATTA GAGGAGGCGG GGGTCCCCGT GGACCTGGTG 
GGCGGCACGT CCATTGGCTC TTTCATCGGA GCGTTGTACG CGGAGGAGCG CAGCGCCAGC 
CGCACGAAGC AGCGGGCCCG GGAGTGGGCC AAGAGCATGA CTTCGGTGCT GGAACCTGTG 
TTGGACCTCA CGTACCCAGT CACCTCCATG TTCACTGGGT CTGCCTTTAA CCGCAGCATC 
CATCGGGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAACGTGACC 
ACAGATATCA CCGCCTCAGC CATGCGAGTC CACAAAGATG GCTCCCTGTG GCGGTACGTG 
CGCGCCAGCA TGACGCTGTC GGGCTACCTG CCCCCGCTGT GCGACCCCAA GGACGGGCAC 
CTACTCATGG ATGGCGGCTA CATCAACAAT CTGCCAGCGG ACATCGCCCG CAGCATGGGT 
GCCAAAACGG TCATCGCCAT TGACGTGGGG AGCCAGGATG AGACGGACCT CAGCACCTAC 
GGGGACAGCC TGTCCGGCTG GTGGCTGCTG TGGAAGCGGC TGAATCCCTG GGCTGACAAG 
GTAAAGGTTC CAGACATGGC TGAAATCCAG TCCCGCCTGG CCTACGTGTC CTGTGTGCGG 
CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC TGCGAGTACC TGCGCCCGCC CATCGACTGC 
TTCAAGACCA TGGACTTTGG GAAGTTCGAC CAGATCTATG ATGTGGGCTA CCAGTACGGG 
AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC AACGTCATTG AGAAAATGCT CACAGACCGG 
CGGTCTACAG ACCTTAATGA GAGCCGCCGT GCAGACGTGC TTGCCTTCCC AAGCTCTGGC 
TTCACTGACT TGGCAGAGAT TGTGTCCCGG ATTGAGCCCC CCACGAGCTA TGTCTCTGAT 
GGCTGTGCTG ACGGAGAGGA GTCAGATTGT CTGACAGAGT ATGAGGAGGA CGCCGGACCC 
GACTGCTCGA GGGATGAAGG GGGGTCCCCC GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG 
ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA CGACGCTGTC TGCCCCAGGA GCCGCCCGGC 
TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159586.1
CDS	87..4184
Translation	MGTSSHGLATNSSGAKVAERDGFQDVLAPGEGSAGRICGAQPVPFVPQVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_001166114.1
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166114.1

ORF Insert Sequence:

ATGGGGACAT CGAGTCACGG GCTGGCTACG AACTCCTCGG GGGCGAAGGT GGCGGAGAGG 
GATGGGTTCC AGGACGTCCT GGCGCCCGGG GAAGGCTCGG CGGGACGGAT TTGCGGTGCG 
CAGCCAGTGC CGTTCGTCCC TCAGGTGCTT GGCGTGATGA TCGGGGCCGG AGTGGCGGTG 
GTGGTCACGG CCGTGCTCAT CCTCCTGGTG GTGCGGAGGC TGCGAGTGCC AAAAACCCCA 
GCCCCGGATG GCCCCCGGTA TCGGTTCCGG AAGAGGGACA AAGTGCTCTT CTATGGCCGG 
AAGATTATGC GGAAGGTGTC ACAATCCACC TCCTCCCTCG TGGATACCTC TGTCTCCGCC 
ACCTCCCGGC CACGCATGAG GAAGAAACTG AAGATGCTCA ACATTGCCAA GAAGATCCTG 
CGCATCCAGA AAGAGACGCC CACGCTGCAG CGGAAGGAGC CCCCGCCCGC AGTGCTAGAA 
GCTGACCTGA CCGAGGGCGA CCTGGCTAAC TCCCATCTGC CCTCTGAAGT GCTTTATATG 
CTCAAGAACG TCCGGGTGCT GGGCCACTTC GAGAAGCCAC TCTTCCTGGA GCTCTGCCGC 
CACATGGTCT TCCAGCGGCT GGGCCAGGGT GACTACGTCT TCCGGCCGGG CCAGCCAGAT 
GCCAGCATCT ACGTGGTGCA GGACGGGCTG CTGGAGCTCT GTCTGCCAGG GCCTGACGGG 
AAGGAGTGTG TGGTGAAGGA AGTGGTTCCT GGGGACAGCG TCAACAGCCT TCTCAGCATC 
CTGGATGTCA TCACCGGTCA CCAGCATCCC CAGCGGACCG TGTCTGCCCG GGCGGCCCGG 
GACTCCACGG TGCTGCGCCT GCCGGTGGAA GCATTCTCCG CGGTCTTCAC CAAGTACCCG 
GAGAGCTTGG TGCGGGTCGT GCAGATCATC ATGGTGCGGC TGCAGCGAGT CACCTTCCTG 
GCACTGCACA ACTACCTGGG TCTGACCAAT GAGCTCTTCA GCCACGAGAT CCAGCCCCTG 
CGTCTGTTCC CCAGCCCCGG CCTCCCAACT CGCACCAGCC CTGTGCGGGG CTCCAAGAGA 
ATGGTCAGCA CCTCAGCTAC AGACGAGCCC AGGGAGACCC CAGGGCGGCC ACCCGATCCC 
ACCGGGGCCC CGCTGCCTGG ACCTACAGGG GACCCTGTGA AGCCCACATC CCTGGAAACC 
CCCTCGGCCC CTCTGCTGAG CCGCTGCGTC TCCATGCCAG GGGACATCTC AGGCTTGCAG 
GGTGGCCCCC GCTCCGACTT CGACATGGCC TATGAGCGTG GCCGGATCTC CGTGTCCCTG 
CAGGAAGAGG CCTCCGGGGG GTCCCTGGCA GCCCCCGCTC GGACCCCCAC TCAGGAGCCT 
CGTGAGCAGC CGGCAGGCGC CTGTGAATAC AGCTACTGTG AGGATGAGTC GGCCACTGGT 
GGCTGCCCTT TCGGGCCCTA CCAGGGCCGC CAGACCAGCA GCATCTTCGA GGCAGCAAAG 
CAGGAGCTGG CCAAGCTGAT GCGGATTGAG GACCCCTCCC TCCTGAACAG CAGAGTCTTG 
CTGCACCACG CCAAAGCTGG CACCATCATT GCCCGCCAGG GAGACCAGGA CGTGAGCCTG 
CACTTCGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATCGACAA GGCGGAGGAC 
GTGTGCCTGT TCGTAGCGCA GCCCGGGGAA CTGGTGGGGC AGCTGGCGGT GCTCACTGGC 
GAACCTCTCA TCTTCACACT GCGAGCCCAA CGCGACTGCA CCTTCCTGCG GATCTCCAAG 
TCCGACTTCT ATGAGATCAT GCGCGCACAG CCCAGTGTGG TGCTGAGTGC GGCGCACACG 
GTGGCAGCCA GGATGTCGCC CTTCGTGCGC CAGATGGACT TCGCCATCGA CTGGACTGCA 
GTGGAGGCGG GACGCGCGCT GTACAGGCAG GGCGACCGCT CCGACTGCAC TTACATCGTG 
CTCAATGGGC GGCTGCGTAG CGTGATCCAG CGAGGCAGTG GCAAGAAGGA GCTGGTGGGC 
GAGTACGGCC GCGGCGACCT CATCGGCGTG GTGGAGGCAC TGACCCGGCA GCCGCGAGCC 
ACGACGGTGC ACGCGGTGCG CGACACGGAG CTGGCCAAGC TTCCCGAGGG CACCTTGGGT 
CACATCAAAC GCCGGTACCC GCAGGTCGTG ACCCGCCTTA TCCACCTACT GAGCCAGAAA 
ATTCTAGGGA ATTTGCAGCA GCTGCAAGGA CCCTTCCCAG GCTCTGGGTT GGGTGTGCCC 
CCACACTCGG AACTCACCAA CCCAGCCAGC AACCTGGCAA CTGTGGCAAT CCTGCCTGTG 
TGTGCTGAGG TCCCCATGGT GGCCTTCACG CTGGAGCTGC AGCACGCCCT GCAGGCCATC 
GGTCCGACGC TACTCCTTAA CAGTGACATC ATCCGGGCAC GCCTGGGGGC CTCCGCACTG 
GATAGCATCC AAGAGTTCCG GCTGTCAGGG TGGCTGGCCC AGCAGGAGGA TGCACACCGT 
ATCGTACTCT ACCAGACGGA CGCCTCGCTG ACGCCCTGGA CCGTGCGCTG CCTGCGACAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAGC CTACCCTCGG CCAGCTGGAG 
CAGATGCTGG AGAACACGGC TGTGCGCGCC CTTAAGCAGC TAGTCCTGCT CCACCGAGAG 
GAGGGCGCGG GCCCCACGCG CACCGTGGAG TGGCTAAATA TGCGCAGCTG GTGCTCGGGG 
CACCTGCACC TGCGCTGTCC GCGCCGCCTC TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT 
GAGCTCTACG AGAAGGTTTT CTCCAGGCGC GCGGACCGGC ACAGCGACTT CTCCCGCTTG 
GCGAGGGTGC TCACGGGGAA CACCATTGCC CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC 
TGCTCGCACA TCGGAGTACT AAAGGCATTA GAGGAGGCGG GGGTCCCCGT GGACCTGGTG 
GGCGGCACGT CCATTGGCTC TTTCATCGGA GCGTTGTACG CGGAGGAGCG CAGCGCCAGC 
CGCACGAAGC AGCGGGCCCG GGAGTGGGCC AAGAGCATGA CTTCGGTGCT GGAACCTGTG 
TTGGACCTCA CGTACCCAGT CACCTCCATG TTCACTGGGT CTGCCTTTAA CCGCAGCATC 
CATCGGGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAACGTGACC 
ACAGATATCA CCGCCTCAGC CATGCGAGTC CACAAAGATG GCTCCCTGTG GCGGTACGTG 
CGCGCCAGCA TGACGCTGTC GGGCTACCTG CCCCCGCTGT GCGACCCCAA GGACGGGCAC 
CTACTCATGG ATGGCGGCTA CATCAACAAT CTGCCAGCGG ACATCGCCCG CAGCATGGGT 
GCCAAAACGG TCATCGCCAT TGACGTGGGG AGCCAGGATG AGACGGACCT CAGCACCTAC 
GGGGACAGCC TGTCCGGCTG GTGGCTGCTG TGGAAGCGGC TGAATCCCTG GGCTGACAAG 
GTAAAGGTTC CAGACATGGC TGAAATCCAG TCCCGCCTGG CCTACGTGTC CTGTGTGCGG 
CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC TGCGAGTACC TGCGCCCGCC CATCGACTGC 
TTCAAGACCA TGGACTTTGG GAAGTTCGAC CAGATCTATG ATGTGGGCTA CCAGTACGGG 
AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC AACGTCATTG AGAAAATGCT CACAGACCGG 
CGGTCTACAG ACCTTAATGA GAGCCGCCGT GCAGACGTGC TTGCCTTCCC AAGCTCTGGC 
TTCACTGACT TGGCAGAGAT TGTGTCCCGG ATTGAGCCCC CCACGAGCTA TGTCTCTGAT 
GGCTGTGCTG ACGGAGAGGA GTCAGATTGT CTGACAGAGT ATGAGGAGGA CGCCGGACCC 
GACTGCTCGA GGGATGAAGG GGGGTCCCCC GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG 
ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA CGACGCTGTC TGCCCCAGGA GCCGCCCGGC 
TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_001166114.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159586.1 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu11758
Clone ID Related Accession (Same CDS sequence)	NM_001166114.1 , NM_001166114.2
Accession Version	NM_001166114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA096881.1, BC038229.1 and BC051768.1. On Jun 2, 2019 this sequence version replaced NM_001166114.1. Transcript Variant: This variant (5) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (d) has a shorter N-terminus and lacks one internal residue, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038229.1, SRR7346977.1960364.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGACAT CGAGTCACGG GCTGGCTACG AACTCCTCGG GGGCGAAGGT GGCGGAGAGG 
GATGGGTTCC AGGACGTCCT GGCGCCCGGG GAAGGCTCGG CGGGACGGAT TTGCGGTGCG 
CAGCCAGTGC CGTTCGTCCC TCAGGTGCTT GGCGTGATGA TCGGGGCCGG AGTGGCGGTG 
GTGGTCACGG CCGTGCTCAT CCTCCTGGTG GTGCGGAGGC TGCGAGTGCC AAAAACCCCA 
GCCCCGGATG GCCCCCGGTA TCGGTTCCGG AAGAGGGACA AAGTGCTCTT CTATGGCCGG 
AAGATTATGC GGAAGGTGTC ACAATCCACC TCCTCCCTCG TGGATACCTC TGTCTCCGCC 
ACCTCCCGGC CACGCATGAG GAAGAAACTG AAGATGCTCA ACATTGCCAA GAAGATCCTG 
CGCATCCAGA AAGAGACGCC CACGCTGCAG CGGAAGGAGC CCCCGCCCGC AGTGCTAGAA 
GCTGACCTGA CCGAGGGCGA CCTGGCTAAC TCCCATCTGC CCTCTGAAGT GCTTTATATG 
CTCAAGAACG TCCGGGTGCT GGGCCACTTC GAGAAGCCAC TCTTCCTGGA GCTCTGCCGC 
CACATGGTCT TCCAGCGGCT GGGCCAGGGT GACTACGTCT TCCGGCCGGG CCAGCCAGAT 
GCCAGCATCT ACGTGGTGCA GGACGGGCTG CTGGAGCTCT GTCTGCCAGG GCCTGACGGG 
AAGGAGTGTG TGGTGAAGGA AGTGGTTCCT GGGGACAGCG TCAACAGCCT TCTCAGCATC 
CTGGATGTCA TCACCGGTCA CCAGCATCCC CAGCGGACCG TGTCTGCCCG GGCGGCCCGG 
GACTCCACGG TGCTGCGCCT GCCGGTGGAA GCATTCTCCG CGGTCTTCAC CAAGTACCCG 
GAGAGCTTGG TGCGGGTCGT GCAGATCATC ATGGTGCGGC TGCAGCGAGT CACCTTCCTG 
GCACTGCACA ACTACCTGGG TCTGACCAAT GAGCTCTTCA GCCACGAGAT CCAGCCCCTG 
CGTCTGTTCC CCAGCCCCGG CCTCCCAACT CGCACCAGCC CTGTGCGGGG CTCCAAGAGA 
ATGGTCAGCA CCTCAGCTAC AGACGAGCCC AGGGAGACCC CAGGGCGGCC ACCCGATCCC 
ACCGGGGCCC CGCTGCCTGG ACCTACAGGG GACCCTGTGA AGCCCACATC CCTGGAAACC 
CCCTCGGCCC CTCTGCTGAG CCGCTGCGTC TCCATGCCAG GGGACATCTC AGGCTTGCAG 
GGTGGCCCCC GCTCCGACTT CGACATGGCC TATGAGCGTG GCCGGATCTC CGTGTCCCTG 
CAGGAAGAGG CCTCCGGGGG GTCCCTGGCA GCCCCCGCTC GGACCCCCAC TCAGGAGCCT 
CGTGAGCAGC CGGCAGGCGC CTGTGAATAC AGCTACTGTG AGGATGAGTC GGCCACTGGT 
GGCTGCCCTT TCGGGCCCTA CCAGGGCCGC CAGACCAGCA GCATCTTCGA GGCAGCAAAG 
CAGGAGCTGG CCAAGCTGAT GCGGATTGAG GACCCCTCCC TCCTGAACAG CAGAGTCTTG 
CTGCACCACG CCAAAGCTGG CACCATCATT GCCCGCCAGG GAGACCAGGA CGTGAGCCTG 
CACTTCGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATCGACAA GGCGGAGGAC 
GTGTGCCTGT TCGTAGCGCA GCCCGGGGAA CTGGTGGGGC AGCTGGCGGT GCTCACTGGC 
GAACCTCTCA TCTTCACACT GCGAGCCCAA CGCGACTGCA CCTTCCTGCG GATCTCCAAG 
TCCGACTTCT ATGAGATCAT GCGCGCACAG CCCAGTGTGG TGCTGAGTGC GGCGCACACG 
GTGGCAGCCA GGATGTCGCC CTTCGTGCGC CAGATGGACT TCGCCATCGA CTGGACTGCA 
GTGGAGGCGG GACGCGCGCT GTACAGGCAG GGCGACCGCT CCGACTGCAC TTACATCGTG 
CTCAATGGGC GGCTGCGTAG CGTGATCCAG CGAGGCAGTG GCAAGAAGGA GCTGGTGGGC 
GAGTACGGCC GCGGCGACCT CATCGGCGTG GTGGAGGCAC TGACCCGGCA GCCGCGAGCC 
ACGACGGTGC ACGCGGTGCG CGACACGGAG CTGGCCAAGC TTCCCGAGGG CACCTTGGGT 
CACATCAAAC GCCGGTACCC GCAGGTCGTG ACCCGCCTTA TCCACCTACT GAGCCAGAAA 
ATTCTAGGGA ATTTGCAGCA GCTGCAAGGA CCCTTCCCAG GCTCTGGGTT GGGTGTGCCC 
CCACACTCGG AACTCACCAA CCCAGCCAGC AACCTGGCAA CTGTGGCAAT CCTGCCTGTG 
TGTGCTGAGG TCCCCATGGT GGCCTTCACG CTGGAGCTGC AGCACGCCCT GCAGGCCATC 
GGTCCGACGC TACTCCTTAA CAGTGACATC ATCCGGGCAC GCCTGGGGGC CTCCGCACTG 
GATAGCATCC AAGAGTTCCG GCTGTCAGGG TGGCTGGCCC AGCAGGAGGA TGCACACCGT 
ATCGTACTCT ACCAGACGGA CGCCTCGCTG ACGCCCTGGA CCGTGCGCTG CCTGCGACAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAGC CTACCCTCGG CCAGCTGGAG 
CAGATGCTGG AGAACACGGC TGTGCGCGCC CTTAAGCAGC TAGTCCTGCT CCACCGAGAG 
GAGGGCGCGG GCCCCACGCG CACCGTGGAG TGGCTAAATA TGCGCAGCTG GTGCTCGGGG 
CACCTGCACC TGCGCTGTCC GCGCCGCCTC TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT 
GAGCTCTACG AGAAGGTTTT CTCCAGGCGC GCGGACCGGC ACAGCGACTT CTCCCGCTTG 
GCGAGGGTGC TCACGGGGAA CACCATTGCC CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC 
TGCTCGCACA TCGGAGTACT AAAGGCATTA GAGGAGGCGG GGGTCCCCGT GGACCTGGTG 
GGCGGCACGT CCATTGGCTC TTTCATCGGA GCGTTGTACG CGGAGGAGCG CAGCGCCAGC 
CGCACGAAGC AGCGGGCCCG GGAGTGGGCC AAGAGCATGA CTTCGGTGCT GGAACCTGTG 
TTGGACCTCA CGTACCCAGT CACCTCCATG TTCACTGGGT CTGCCTTTAA CCGCAGCATC 
CATCGGGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAACGTGACC 
ACAGATATCA CCGCCTCAGC CATGCGAGTC CACAAAGATG GCTCCCTGTG GCGGTACGTG 
CGCGCCAGCA TGACGCTGTC GGGCTACCTG CCCCCGCTGT GCGACCCCAA GGACGGGCAC 
CTACTCATGG ATGGCGGCTA CATCAACAAT CTGCCAGCGG ACATCGCCCG CAGCATGGGT 
GCCAAAACGG TCATCGCCAT TGACGTGGGG AGCCAGGATG AGACGGACCT CAGCACCTAC 
GGGGACAGCC TGTCCGGCTG GTGGCTGCTG TGGAAGCGGC TGAATCCCTG GGCTGACAAG 
GTAAAGGTTC CAGACATGGC TGAAATCCAG TCCCGCCTGG CCTACGTGTC CTGTGTGCGG 
CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC TGCGAGTACC TGCGCCCGCC CATCGACTGC 
TTCAAGACCA TGGACTTTGG GAAGTTCGAC CAGATCTATG ATGTGGGCTA CCAGTACGGG 
AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC AACGTCATTG AGAAAATGCT CACAGACCGG 
CGGTCTACAG ACCTTAATGA GAGCCGCCGT GCAGACGTGC TTGCCTTCCC AAGCTCTGGC 
TTCACTGACT TGGCAGAGAT TGTGTCCCGG ATTGAGCCCC CCACGAGCTA TGTCTCTGAT 
GGCTGTGCTG ACGGAGAGGA GTCAGATTGT CTGACAGAGT ATGAGGAGGA CGCCGGACCC 
GACTGCTCGA GGGATGAAGG GGGGTCCCCC GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG 
ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA CGACGCTGTC TGCCCCAGGA GCCGCCCGGC 
TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159586.1
CDS	73..4170
Translation	MGTSSHGLATNSSGAKVAERDGFQDVLAPGEGSAGRICGAQPVPFVPQVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_001166114.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166114.2

ORF Insert Sequence:

ATGGGGACAT CGAGTCACGG GCTGGCTACG AACTCCTCGG GGGCGAAGGT GGCGGAGAGG 
GATGGGTTCC AGGACGTCCT GGCGCCCGGG GAAGGCTCGG CGGGACGGAT TTGCGGTGCG 
CAGCCAGTGC CGTTCGTCCC TCAGGTGCTT GGCGTGATGA TCGGGGCCGG AGTGGCGGTG 
GTGGTCACGG CCGTGCTCAT CCTCCTGGTG GTGCGGAGGC TGCGAGTGCC AAAAACCCCA 
GCCCCGGATG GCCCCCGGTA TCGGTTCCGG AAGAGGGACA AAGTGCTCTT CTATGGCCGG 
AAGATTATGC GGAAGGTGTC ACAATCCACC TCCTCCCTCG TGGATACCTC TGTCTCCGCC 
ACCTCCCGGC CACGCATGAG GAAGAAACTG AAGATGCTCA ACATTGCCAA GAAGATCCTG 
CGCATCCAGA AAGAGACGCC CACGCTGCAG CGGAAGGAGC CCCCGCCCGC AGTGCTAGAA 
GCTGACCTGA CCGAGGGCGA CCTGGCTAAC TCCCATCTGC CCTCTGAAGT GCTTTATATG 
CTCAAGAACG TCCGGGTGCT GGGCCACTTC GAGAAGCCAC TCTTCCTGGA GCTCTGCCGC 
CACATGGTCT TCCAGCGGCT GGGCCAGGGT GACTACGTCT TCCGGCCGGG CCAGCCAGAT 
GCCAGCATCT ACGTGGTGCA GGACGGGCTG CTGGAGCTCT GTCTGCCAGG GCCTGACGGG 
AAGGAGTGTG TGGTGAAGGA AGTGGTTCCT GGGGACAGCG TCAACAGCCT TCTCAGCATC 
CTGGATGTCA TCACCGGTCA CCAGCATCCC CAGCGGACCG TGTCTGCCCG GGCGGCCCGG 
GACTCCACGG TGCTGCGCCT GCCGGTGGAA GCATTCTCCG CGGTCTTCAC CAAGTACCCG 
GAGAGCTTGG TGCGGGTCGT GCAGATCATC ATGGTGCGGC TGCAGCGAGT CACCTTCCTG 
GCACTGCACA ACTACCTGGG TCTGACCAAT GAGCTCTTCA GCCACGAGAT CCAGCCCCTG 
CGTCTGTTCC CCAGCCCCGG CCTCCCAACT CGCACCAGCC CTGTGCGGGG CTCCAAGAGA 
ATGGTCAGCA CCTCAGCTAC AGACGAGCCC AGGGAGACCC CAGGGCGGCC ACCCGATCCC 
ACCGGGGCCC CGCTGCCTGG ACCTACAGGG GACCCTGTGA AGCCCACATC CCTGGAAACC 
CCCTCGGCCC CTCTGCTGAG CCGCTGCGTC TCCATGCCAG GGGACATCTC AGGCTTGCAG 
GGTGGCCCCC GCTCCGACTT CGACATGGCC TATGAGCGTG GCCGGATCTC CGTGTCCCTG 
CAGGAAGAGG CCTCCGGGGG GTCCCTGGCA GCCCCCGCTC GGACCCCCAC TCAGGAGCCT 
CGTGAGCAGC CGGCAGGCGC CTGTGAATAC AGCTACTGTG AGGATGAGTC GGCCACTGGT 
GGCTGCCCTT TCGGGCCCTA CCAGGGCCGC CAGACCAGCA GCATCTTCGA GGCAGCAAAG 
CAGGAGCTGG CCAAGCTGAT GCGGATTGAG GACCCCTCCC TCCTGAACAG CAGAGTCTTG 
CTGCACCACG CCAAAGCTGG CACCATCATT GCCCGCCAGG GAGACCAGGA CGTGAGCCTG 
CACTTCGTGC TCTGGGGCTG CCTGCACGTG TACCAGCGCA TGATCGACAA GGCGGAGGAC 
GTGTGCCTGT TCGTAGCGCA GCCCGGGGAA CTGGTGGGGC AGCTGGCGGT GCTCACTGGC 
GAACCTCTCA TCTTCACACT GCGAGCCCAA CGCGACTGCA CCTTCCTGCG GATCTCCAAG 
TCCGACTTCT ATGAGATCAT GCGCGCACAG CCCAGTGTGG TGCTGAGTGC GGCGCACACG 
GTGGCAGCCA GGATGTCGCC CTTCGTGCGC CAGATGGACT TCGCCATCGA CTGGACTGCA 
GTGGAGGCGG GACGCGCGCT GTACAGGCAG GGCGACCGCT CCGACTGCAC TTACATCGTG 
CTCAATGGGC GGCTGCGTAG CGTGATCCAG CGAGGCAGTG GCAAGAAGGA GCTGGTGGGC 
GAGTACGGCC GCGGCGACCT CATCGGCGTG GTGGAGGCAC TGACCCGGCA GCCGCGAGCC 
ACGACGGTGC ACGCGGTGCG CGACACGGAG CTGGCCAAGC TTCCCGAGGG CACCTTGGGT 
CACATCAAAC GCCGGTACCC GCAGGTCGTG ACCCGCCTTA TCCACCTACT GAGCCAGAAA 
ATTCTAGGGA ATTTGCAGCA GCTGCAAGGA CCCTTCCCAG GCTCTGGGTT GGGTGTGCCC 
CCACACTCGG AACTCACCAA CCCAGCCAGC AACCTGGCAA CTGTGGCAAT CCTGCCTGTG 
TGTGCTGAGG TCCCCATGGT GGCCTTCACG CTGGAGCTGC AGCACGCCCT GCAGGCCATC 
GGTCCGACGC TACTCCTTAA CAGTGACATC ATCCGGGCAC GCCTGGGGGC CTCCGCACTG 
GATAGCATCC AAGAGTTCCG GCTGTCAGGG TGGCTGGCCC AGCAGGAGGA TGCACACCGT 
ATCGTACTCT ACCAGACGGA CGCCTCGCTG ACGCCCTGGA CCGTGCGCTG CCTGCGACAG 
GCCGACTGCA TCCTCATTGT GGGCCTGGGG GACCAGGAGC CTACCCTCGG CCAGCTGGAG 
CAGATGCTGG AGAACACGGC TGTGCGCGCC CTTAAGCAGC TAGTCCTGCT CCACCGAGAG 
GAGGGCGCGG GCCCCACGCG CACCGTGGAG TGGCTAAATA TGCGCAGCTG GTGCTCGGGG 
CACCTGCACC TGCGCTGTCC GCGCCGCCTC TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT 
GAGCTCTACG AGAAGGTTTT CTCCAGGCGC GCGGACCGGC ACAGCGACTT CTCCCGCTTG 
GCGAGGGTGC TCACGGGGAA CACCATTGCC CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC 
TGCTCGCACA TCGGAGTACT AAAGGCATTA GAGGAGGCGG GGGTCCCCGT GGACCTGGTG 
GGCGGCACGT CCATTGGCTC TTTCATCGGA GCGTTGTACG CGGAGGAGCG CAGCGCCAGC 
CGCACGAAGC AGCGGGCCCG GGAGTGGGCC AAGAGCATGA CTTCGGTGCT GGAACCTGTG 
TTGGACCTCA CGTACCCAGT CACCTCCATG TTCACTGGGT CTGCCTTTAA CCGCAGCATC 
CATCGGGTCT TCCAGGATAA GCAGATTGAG GACCTGTGGC TGCCTTACTT CAACGTGACC 
ACAGATATCA CCGCCTCAGC CATGCGAGTC CACAAAGATG GCTCCCTGTG GCGGTACGTG 
CGCGCCAGCA TGACGCTGTC GGGCTACCTG CCCCCGCTGT GCGACCCCAA GGACGGGCAC 
CTACTCATGG ATGGCGGCTA CATCAACAAT CTGCCAGCGG ACATCGCCCG CAGCATGGGT 
GCCAAAACGG TCATCGCCAT TGACGTGGGG AGCCAGGATG AGACGGACCT CAGCACCTAC 
GGGGACAGCC TGTCCGGCTG GTGGCTGCTG TGGAAGCGGC TGAATCCCTG GGCTGACAAG 
GTAAAGGTTC CAGACATGGC TGAAATCCAG TCCCGCCTGG CCTACGTGTC CTGTGTGCGG 
CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC TGCGAGTACC TGCGCCCGCC CATCGACTGC 
TTCAAGACCA TGGACTTTGG GAAGTTCGAC CAGATCTATG ATGTGGGCTA CCAGTACGGG 
AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC AACGTCATTG AGAAAATGCT CACAGACCGG 
CGGTCTACAG ACCTTAATGA GAGCCGCCGT GCAGACGTGC TTGCCTTCCC AAGCTCTGGC 
TTCACTGACT TGGCAGAGAT TGTGTCCCGG ATTGAGCCCC CCACGAGCTA TGTCTCTGAT 
GGCTGTGCTG ACGGAGAGGA GTCAGATTGT CTGACAGAGT ATGAGGAGGA CGCCGGACCC 
GACTGCTCGA GGGATGAAGG GGGGTCCCCC GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG 
ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA CGACGCTGTC TGCCCCAGGA GCCGCCCGGC 
TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_001166111.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159583.1 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 1, mRNA.

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CloneID	OHu12332
Clone ID Related Accession (Same CDS sequence)	NM_001166111.2 , NM_001166111.1
Accession Version	NM_001166111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068563.1, AK294021.1, BC038229.1 and BC051768.1. On Jun 2, 2019 this sequence version replaced NM_001166111.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294021.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTC CGCTGCAAAC TGGAATGATG GGGACATCGA GTCACGGGCT GGCTACGAAC 
TCCTCGGGGG CGAAGGTGGC GGAGAGGGAT GGGTTCCAGG ACGTCCTGGC GCCCGGGGAA 
GGCTCGGCGG GACGGATTTG CGGTGCGCAG CCAGTGCCGT TCGTCCCTCA GGTGCTTGGC 
GTGATGATCG GGGCCGGAGT GGCGGTGGTG GTCACGGCCG TGCTCATCCT CCTGGTGGTG 
CGGAGGCTGC GAGTGCCAAA AACCCCAGCC CCGGATGGCC CCCGGTATCG GTTCCGGAAG 
AGGGACAAAG TGCTCTTCTA TGGCCGGAAG ATTATGCGGA AGGTGTCACA ATCCACCTCC 
TCCCTCGTGG ATACCTCTGT CTCCGCCACC TCCCGGCCAC GCATGAGGAA GAAACTGAAG 
ATGCTCAACA TTGCCAAGAA GATCCTGCGC ATCCAGAAAG AGACGCCCAC GCTGCAGCGG 
AAGGAGCCCC CGCCCGCAGT GCTAGAAGCT GACCTGACCG AGGGCGACCT GGCTAACTCC 
CATCTGCCCT CTGAAGTGCT TTATATGCTC AAGAACGTCC GGGTGCTGGG CCACTTCGAG 
AAGCCACTCT TCCTGGAGCT CTGCCGCCAC ATGGTCTTCC AGCGGCTGGG CCAGGGTGAC 
TACGTCTTCC GGCCGGGCCA GCCAGATGCC AGCATCTACG TGGTGCAGGA CGGGCTGCTG 
GAGCTCTGTC TGCCAGGGCC TGACGGGAAG GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG 
GACAGCGTCA ACAGCCTTCT CAGCATCCTG GATGTCATCA CCGGTCACCA GCATCCCCAG 
CGGACCGTGT CTGCCCGGGC GGCCCGGGAC TCCACGGTGC TGCGCCTGCC GGTGGAAGCA 
TTCTCCGCGG TCTTCACCAA GTACCCGGAG AGCTTGGTGC GGGTCGTGCA GATCATCATG 
GTGCGGCTGC AGCGAGTCAC CTTCCTGGCA CTGCACAACT ACCTGGGTCT GACCAATGAG 
CTCTTCAGCC ACGAGATCCA GCCCCTGCGT CTGTTCCCCA GCCCCGGCCT CCCAACTCGC 
ACCAGCCCTG TGCGGGGCTC CAAGAGAATG GTCAGCACCT CAGCTACAGA CGAGCCCAGG 
GAGACCCCAG GGCGGCCACC CGATCCCACC GGGGCCCCGC TGCCTGGACC TACAGGGGAC 
CCTGTGAAGC CCACATCCCT GGAAACCCCC TCGGCCCCTC TGCTGAGCCG CTGCGTCTCC 
ATGCCAGGGG ACATCTCAGG CTTGCAGGGT GGCCCCCGCT CCGACTTCGA CATGGCCTAT 
GAGCGTGGCC GGATCTCCGT GTCCCTGCAG GAAGAGGCCT CCGGGGGGTC CCTGGCAGCC 
CCCGCTCGGA CCCCCACTCA GGAGCCTCGT GAGCAGCCGG CAGGCGCCTG TGAATACAGC 
TACTGTGAGG ATGAGTCGGC CACTGGTGGC TGCCCTTTCG GGCCCTACCA GGGCCGCCAG 
ACCAGCAGCA TCTTCGAGGC AGCAAAGCAG GAGCTGGCCA AGCTGATGCG GATTGAGGAC 
CCCTCCCTCC TGAACAGCAG AGTCTTGCTG CACCACGCCA AAGCTGGCAC CATCATTGCC 
CGCCAGGGAG ACCAGGACGT GAGCCTGCAC TTCGTGCTCT GGGGCTGCCT GCACGTGTAC 
CAGCGCATGA TCGACAAGGC GGAGGACGTG TGCCTGTTCG TAGCGCAGCC CGGGGAACTG 
GTGGGGCAGC TGGCGGTGCT CACTGGCGAA CCTCTCATCT TCACACTGCG AGCCCAACGC 
GACTGCACCT TCCTGCGGAT CTCCAAGTCC GACTTCTATG AGATCATGCG CGCACAGCCC 
AGTGTGGTGC TGAGTGCGGC GCACACGGTG GCAGCCAGGA TGTCGCCCTT CGTGCGCCAG 
ATGGACTTCG CCATCGACTG GACTGCAGTG GAGGCGGGAC GCGCGCTGTA CAGGCAGGGC 
GACCGCTCCG ACTGCACTTA CATCGTGCTC AATGGGCGGC TGCGTAGCGT GATCCAGCGA 
GGCAGTGGCA AGAAGGAGCT GGTGGGCGAG TACGGCCGCG GCGACCTCAT CGGCGTGGTG 
GAGGCACTGA CCCGGCAGCC GCGAGCCACG ACGGTGCACG CGGTGCGCGA CACGGAGCTG 
GCCAAGCTTC CCGAGGGCAC CTTGGGTCAC ATCAAACGCC GGTACCCGCA GGTCGTGACC 
CGCCTTATCC ACCTACTGAG CCAGAAAATT CTAGGGAATT TGCAGCAGCT GCAAGGACCC 
TTCCCAGCAG GCTCTGGGTT GGGTGTGCCC CCACACTCGG AACTCACCAA CCCAGCCAGC 
AACCTGGCAA CTGTGGCAAT CCTGCCTGTG TGTGCTGAGG TCCCCATGGT GGCCTTCACG 
CTGGAGCTGC AGCACGCCCT GCAGGCCATC GGTCCGACGC TACTCCTTAA CAGTGACATC 
ATCCGGGCAC GCCTGGGGGC CTCCGCACTG GATAGCATCC AAGAGTTCCG GCTGTCAGGG 
TGGCTGGCCC AGCAGGAGGA TGCACACCGT ATCGTACTCT ACCAGACGGA CGCCTCGCTG 
ACGCCCTGGA CCGTGCGCTG CCTGCGACAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAGC CTACCCTCGG CCAGCTGGAG CAGATGCTGG AGAACACGGC TGTGCGCGCC 
CTTAAGCAGC TAGTCCTGCT CCACCGAGAG GAGGGCGCGG GCCCCACGCG CACCGTGGAG 
TGGCTAAATA TGCGCAGCTG GTGCTCGGGG CACCTGCACC TGCGCTGTCC GCGCCGCCTC 
TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT GAGCTCTACG AGAAGGTTTT CTCCAGGCGC 
GCGGACCGGC ACAGCGACTT CTCCCGCTTG GCGAGGGTGC TCACGGGGAA CACCATTGCC 
CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC TGCTCGCACA TCGGAGTACT AAAGGCATTA 
GAGGAGGCGG GGGTCCCCGT GGACCTGGTG GGCGGCACGT CCATTGGCTC TTTCATCGGA 
GCGTTGTACG CGGAGGAGCG CAGCGCCAGC CGCACGAAGC AGCGGGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCGGTGCT GGAACCTGTG TTGGACCTCA CGTACCCAGT CACCTCCATG 
TTCACTGGGT CTGCCTTTAA CCGCAGCATC CATCGGGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAACGTGACC ACAGATATCA CCGCCTCAGC CATGCGAGTC 
CACAAAGATG GCTCCCTGTG GCGGTACGTG CGCGCCAGCA TGACGCTGTC GGGCTACCTG 
CCCCCGCTGT GCGACCCCAA GGACGGGCAC CTACTCATGG ATGGCGGCTA CATCAACAAT 
CTGCCAGCGG ACATCGCCCG CAGCATGGGT GCCAAAACGG TCATCGCCAT TGACGTGGGG 
AGCCAGGATG AGACGGACCT CAGCACCTAC GGGGACAGCC TGTCCGGCTG GTGGCTGCTG 
TGGAAGCGGC TGAATCCCTG GGCTGACAAG GTAAAGGTTC CAGACATGGC TGAAATCCAG 
TCCCGCCTGG CCTACGTGTC CTGTGTGCGG CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC 
TGCGAGTACC TGCGCCCGCC CATCGACTGC TTCAAGACCA TGGACTTTGG GAAGTTCGAC 
CAGATCTATG ATGTGGGCTA CCAGTACGGG AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC 
AACGTCATTG AGAAAATGCT CACAGACCGG CGGTCTACAG ACCTTAATGA GAGCCGCCGT 
GCAGACGTGC TTGCCTTCCC AAGCTCTGGC TTCACTGACT TGGCAGAGAT TGTGTCCCGG 
ATTGAGCCCC CCACGAGCTA TGTCTCTGAT GGCTGTGCTG ACGGAGAGGA GTCAGATTGT 
CTGACAGAGT ATGAGGAGGA CGCCGGACCC GACTGCTCGA GGGATGAAGG GGGGTCCCCC 
GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA 
CGACGCTGTC TGCCCCAGGA GCCGCCCGGC TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159583.1
CDS	207..4334
Translation	MEAPLQTGMMGTSSHGLATNSSGAKVAERDGFQDVLAPGEGSAGRICGAQPVPFVPQVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPAGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_001166111.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166111.2

ORF Insert Sequence:

ATGGAGGCTC CGCTGCAAAC TGGAATGATG GGGACATCGA GTCACGGGCT GGCTACGAAC 
TCCTCGGGGG CGAAGGTGGC GGAGAGGGAT GGGTTCCAGG ACGTCCTGGC GCCCGGGGAA 
GGCTCGGCGG GACGGATTTG CGGTGCGCAG CCAGTGCCGT TCGTCCCTCA GGTGCTTGGC 
GTGATGATCG GGGCCGGAGT GGCGGTGGTG GTCACGGCCG TGCTCATCCT CCTGGTGGTG 
CGGAGGCTGC GAGTGCCAAA AACCCCAGCC CCGGATGGCC CCCGGTATCG GTTCCGGAAG 
AGGGACAAAG TGCTCTTCTA TGGCCGGAAG ATTATGCGGA AGGTGTCACA ATCCACCTCC 
TCCCTCGTGG ATACCTCTGT CTCCGCCACC TCCCGGCCAC GCATGAGGAA GAAACTGAAG 
ATGCTCAACA TTGCCAAGAA GATCCTGCGC ATCCAGAAAG AGACGCCCAC GCTGCAGCGG 
AAGGAGCCCC CGCCCGCAGT GCTAGAAGCT GACCTGACCG AGGGCGACCT GGCTAACTCC 
CATCTGCCCT CTGAAGTGCT TTATATGCTC AAGAACGTCC GGGTGCTGGG CCACTTCGAG 
AAGCCACTCT TCCTGGAGCT CTGCCGCCAC ATGGTCTTCC AGCGGCTGGG CCAGGGTGAC 
TACGTCTTCC GGCCGGGCCA GCCAGATGCC AGCATCTACG TGGTGCAGGA CGGGCTGCTG 
GAGCTCTGTC TGCCAGGGCC TGACGGGAAG GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG 
GACAGCGTCA ACAGCCTTCT CAGCATCCTG GATGTCATCA CCGGTCACCA GCATCCCCAG 
CGGACCGTGT CTGCCCGGGC GGCCCGGGAC TCCACGGTGC TGCGCCTGCC GGTGGAAGCA 
TTCTCCGCGG TCTTCACCAA GTACCCGGAG AGCTTGGTGC GGGTCGTGCA GATCATCATG 
GTGCGGCTGC AGCGAGTCAC CTTCCTGGCA CTGCACAACT ACCTGGGTCT GACCAATGAG 
CTCTTCAGCC ACGAGATCCA GCCCCTGCGT CTGTTCCCCA GCCCCGGCCT CCCAACTCGC 
ACCAGCCCTG TGCGGGGCTC CAAGAGAATG GTCAGCACCT CAGCTACAGA CGAGCCCAGG 
GAGACCCCAG GGCGGCCACC CGATCCCACC GGGGCCCCGC TGCCTGGACC TACAGGGGAC 
CCTGTGAAGC CCACATCCCT GGAAACCCCC TCGGCCCCTC TGCTGAGCCG CTGCGTCTCC 
ATGCCAGGGG ACATCTCAGG CTTGCAGGGT GGCCCCCGCT CCGACTTCGA CATGGCCTAT 
GAGCGTGGCC GGATCTCCGT GTCCCTGCAG GAAGAGGCCT CCGGGGGGTC CCTGGCAGCC 
CCCGCTCGGA CCCCCACTCA GGAGCCTCGT GAGCAGCCGG CAGGCGCCTG TGAATACAGC 
TACTGTGAGG ATGAGTCGGC CACTGGTGGC TGCCCTTTCG GGCCCTACCA GGGCCGCCAG 
ACCAGCAGCA TCTTCGAGGC AGCAAAGCAG GAGCTGGCCA AGCTGATGCG GATTGAGGAC 
CCCTCCCTCC TGAACAGCAG AGTCTTGCTG CACCACGCCA AAGCTGGCAC CATCATTGCC 
CGCCAGGGAG ACCAGGACGT GAGCCTGCAC TTCGTGCTCT GGGGCTGCCT GCACGTGTAC 
CAGCGCATGA TCGACAAGGC GGAGGACGTG TGCCTGTTCG TAGCGCAGCC CGGGGAACTG 
GTGGGGCAGC TGGCGGTGCT CACTGGCGAA CCTCTCATCT TCACACTGCG AGCCCAACGC 
GACTGCACCT TCCTGCGGAT CTCCAAGTCC GACTTCTATG AGATCATGCG CGCACAGCCC 
AGTGTGGTGC TGAGTGCGGC GCACACGGTG GCAGCCAGGA TGTCGCCCTT CGTGCGCCAG 
ATGGACTTCG CCATCGACTG GACTGCAGTG GAGGCGGGAC GCGCGCTGTA CAGGCAGGGC 
GACCGCTCCG ACTGCACTTA CATCGTGCTC AATGGGCGGC TGCGTAGCGT GATCCAGCGA 
GGCAGTGGCA AGAAGGAGCT GGTGGGCGAG TACGGCCGCG GCGACCTCAT CGGCGTGGTG 
GAGGCACTGA CCCGGCAGCC GCGAGCCACG ACGGTGCACG CGGTGCGCGA CACGGAGCTG 
GCCAAGCTTC CCGAGGGCAC CTTGGGTCAC ATCAAACGCC GGTACCCGCA GGTCGTGACC 
CGCCTTATCC ACCTACTGAG CCAGAAAATT CTAGGGAATT TGCAGCAGCT GCAAGGACCC 
TTCCCAGCAG GCTCTGGGTT GGGTGTGCCC CCACACTCGG AACTCACCAA CCCAGCCAGC 
AACCTGGCAA CTGTGGCAAT CCTGCCTGTG TGTGCTGAGG TCCCCATGGT GGCCTTCACG 
CTGGAGCTGC AGCACGCCCT GCAGGCCATC GGTCCGACGC TACTCCTTAA CAGTGACATC 
ATCCGGGCAC GCCTGGGGGC CTCCGCACTG GATAGCATCC AAGAGTTCCG GCTGTCAGGG 
TGGCTGGCCC AGCAGGAGGA TGCACACCGT ATCGTACTCT ACCAGACGGA CGCCTCGCTG 
ACGCCCTGGA CCGTGCGCTG CCTGCGACAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAGC CTACCCTCGG CCAGCTGGAG CAGATGCTGG AGAACACGGC TGTGCGCGCC 
CTTAAGCAGC TAGTCCTGCT CCACCGAGAG GAGGGCGCGG GCCCCACGCG CACCGTGGAG 
TGGCTAAATA TGCGCAGCTG GTGCTCGGGG CACCTGCACC TGCGCTGTCC GCGCCGCCTC 
TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT GAGCTCTACG AGAAGGTTTT CTCCAGGCGC 
GCGGACCGGC ACAGCGACTT CTCCCGCTTG GCGAGGGTGC TCACGGGGAA CACCATTGCC 
CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC TGCTCGCACA TCGGAGTACT AAAGGCATTA 
GAGGAGGCGG GGGTCCCCGT GGACCTGGTG GGCGGCACGT CCATTGGCTC TTTCATCGGA 
GCGTTGTACG CGGAGGAGCG CAGCGCCAGC CGCACGAAGC AGCGGGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCGGTGCT GGAACCTGTG TTGGACCTCA CGTACCCAGT CACCTCCATG 
TTCACTGGGT CTGCCTTTAA CCGCAGCATC CATCGGGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAACGTGACC ACAGATATCA CCGCCTCAGC CATGCGAGTC 
CACAAAGATG GCTCCCTGTG GCGGTACGTG CGCGCCAGCA TGACGCTGTC GGGCTACCTG 
CCCCCGCTGT GCGACCCCAA GGACGGGCAC CTACTCATGG ATGGCGGCTA CATCAACAAT 
CTGCCAGCGG ACATCGCCCG CAGCATGGGT GCCAAAACGG TCATCGCCAT TGACGTGGGG 
AGCCAGGATG AGACGGACCT CAGCACCTAC GGGGACAGCC TGTCCGGCTG GTGGCTGCTG 
TGGAAGCGGC TGAATCCCTG GGCTGACAAG GTAAAGGTTC CAGACATGGC TGAAATCCAG 
TCCCGCCTGG CCTACGTGTC CTGTGTGCGG CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC 
TGCGAGTACC TGCGCCCGCC CATCGACTGC TTCAAGACCA TGGACTTTGG GAAGTTCGAC 
CAGATCTATG ATGTGGGCTA CCAGTACGGG AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC 
AACGTCATTG AGAAAATGCT CACAGACCGG CGGTCTACAG ACCTTAATGA GAGCCGCCGT 
GCAGACGTGC TTGCCTTCCC AAGCTCTGGC TTCACTGACT TGGCAGAGAT TGTGTCCCGG 
ATTGAGCCCC CCACGAGCTA TGTCTCTGAT GGCTGTGCTG ACGGAGAGGA GTCAGATTGT 
CTGACAGAGT ATGAGGAGGA CGCCGGACCC GACTGCTCGA GGGATGAAGG GGGGTCCCCC 
GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA 
CGACGCTGTC TGCCCCAGGA GCCGCCCGGC TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPLA6 ( NM_001166111.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159583.1 Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 1, mRNA.

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CloneID	OHu12332
Clone ID Related Accession (Same CDS sequence)	NM_001166111.2 , NM_001166111.1
Accession Version	NM_001166111.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	neuropathy target esterase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068563.1, AK294021.1, BC038229.1, BC051768.1 and BQ001677.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294021.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCTC CGCTGCAAAC TGGAATGATG GGGACATCGA GTCACGGGCT GGCTACGAAC 
TCCTCGGGGG CGAAGGTGGC GGAGAGGGAT GGGTTCCAGG ACGTCCTGGC GCCCGGGGAA 
GGCTCGGCGG GACGGATTTG CGGTGCGCAG CCAGTGCCGT TCGTCCCTCA GGTGCTTGGC 
GTGATGATCG GGGCCGGAGT GGCGGTGGTG GTCACGGCCG TGCTCATCCT CCTGGTGGTG 
CGGAGGCTGC GAGTGCCAAA AACCCCAGCC CCGGATGGCC CCCGGTATCG GTTCCGGAAG 
AGGGACAAAG TGCTCTTCTA TGGCCGGAAG ATTATGCGGA AGGTGTCACA ATCCACCTCC 
TCCCTCGTGG ATACCTCTGT CTCCGCCACC TCCCGGCCAC GCATGAGGAA GAAACTGAAG 
ATGCTCAACA TTGCCAAGAA GATCCTGCGC ATCCAGAAAG AGACGCCCAC GCTGCAGCGG 
AAGGAGCCCC CGCCCGCAGT GCTAGAAGCT GACCTGACCG AGGGCGACCT GGCTAACTCC 
CATCTGCCCT CTGAAGTGCT TTATATGCTC AAGAACGTCC GGGTGCTGGG CCACTTCGAG 
AAGCCACTCT TCCTGGAGCT CTGCCGCCAC ATGGTCTTCC AGCGGCTGGG CCAGGGTGAC 
TACGTCTTCC GGCCGGGCCA GCCAGATGCC AGCATCTACG TGGTGCAGGA CGGGCTGCTG 
GAGCTCTGTC TGCCAGGGCC TGACGGGAAG GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG 
GACAGCGTCA ACAGCCTTCT CAGCATCCTG GATGTCATCA CCGGTCACCA GCATCCCCAG 
CGGACCGTGT CTGCCCGGGC GGCCCGGGAC TCCACGGTGC TGCGCCTGCC GGTGGAAGCA 
TTCTCCGCGG TCTTCACCAA GTACCCGGAG AGCTTGGTGC GGGTCGTGCA GATCATCATG 
GTGCGGCTGC AGCGAGTCAC CTTCCTGGCA CTGCACAACT ACCTGGGTCT GACCAATGAG 
CTCTTCAGCC ACGAGATCCA GCCCCTGCGT CTGTTCCCCA GCCCCGGCCT CCCAACTCGC 
ACCAGCCCTG TGCGGGGCTC CAAGAGAATG GTCAGCACCT CAGCTACAGA CGAGCCCAGG 
GAGACCCCAG GGCGGCCACC CGATCCCACC GGGGCCCCGC TGCCTGGACC TACAGGGGAC 
CCTGTGAAGC CCACATCCCT GGAAACCCCC TCGGCCCCTC TGCTGAGCCG CTGCGTCTCC 
ATGCCAGGGG ACATCTCAGG CTTGCAGGGT GGCCCCCGCT CCGACTTCGA CATGGCCTAT 
GAGCGTGGCC GGATCTCCGT GTCCCTGCAG GAAGAGGCCT CCGGGGGGTC CCTGGCAGCC 
CCCGCTCGGA CCCCCACTCA GGAGCCTCGT GAGCAGCCGG CAGGCGCCTG TGAATACAGC 
TACTGTGAGG ATGAGTCGGC CACTGGTGGC TGCCCTTTCG GGCCCTACCA GGGCCGCCAG 
ACCAGCAGCA TCTTCGAGGC AGCAAAGCAG GAGCTGGCCA AGCTGATGCG GATTGAGGAC 
CCCTCCCTCC TGAACAGCAG AGTCTTGCTG CACCACGCCA AAGCTGGCAC CATCATTGCC 
CGCCAGGGAG ACCAGGACGT GAGCCTGCAC TTCGTGCTCT GGGGCTGCCT GCACGTGTAC 
CAGCGCATGA TCGACAAGGC GGAGGACGTG TGCCTGTTCG TAGCGCAGCC CGGGGAACTG 
GTGGGGCAGC TGGCGGTGCT CACTGGCGAA CCTCTCATCT TCACACTGCG AGCCCAACGC 
GACTGCACCT TCCTGCGGAT CTCCAAGTCC GACTTCTATG AGATCATGCG CGCACAGCCC 
AGTGTGGTGC TGAGTGCGGC GCACACGGTG GCAGCCAGGA TGTCGCCCTT CGTGCGCCAG 
ATGGACTTCG CCATCGACTG GACTGCAGTG GAGGCGGGAC GCGCGCTGTA CAGGCAGGGC 
GACCGCTCCG ACTGCACTTA CATCGTGCTC AATGGGCGGC TGCGTAGCGT GATCCAGCGA 
GGCAGTGGCA AGAAGGAGCT GGTGGGCGAG TACGGCCGCG GCGACCTCAT CGGCGTGGTG 
GAGGCACTGA CCCGGCAGCC GCGAGCCACG ACGGTGCACG CGGTGCGCGA CACGGAGCTG 
GCCAAGCTTC CCGAGGGCAC CTTGGGTCAC ATCAAACGCC GGTACCCGCA GGTCGTGACC 
CGCCTTATCC ACCTACTGAG CCAGAAAATT CTAGGGAATT TGCAGCAGCT GCAAGGACCC 
TTCCCAGCAG GCTCTGGGTT GGGTGTGCCC CCACACTCGG AACTCACCAA CCCAGCCAGC 
AACCTGGCAA CTGTGGCAAT CCTGCCTGTG TGTGCTGAGG TCCCCATGGT GGCCTTCACG 
CTGGAGCTGC AGCACGCCCT GCAGGCCATC GGTCCGACGC TACTCCTTAA CAGTGACATC 
ATCCGGGCAC GCCTGGGGGC CTCCGCACTG GATAGCATCC AAGAGTTCCG GCTGTCAGGG 
TGGCTGGCCC AGCAGGAGGA TGCACACCGT ATCGTACTCT ACCAGACGGA CGCCTCGCTG 
ACGCCCTGGA CCGTGCGCTG CCTGCGACAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAGC CTACCCTCGG CCAGCTGGAG CAGATGCTGG AGAACACGGC TGTGCGCGCC 
CTTAAGCAGC TAGTCCTGCT CCACCGAGAG GAGGGCGCGG GCCCCACGCG CACCGTGGAG 
TGGCTAAATA TGCGCAGCTG GTGCTCGGGG CACCTGCACC TGCGCTGTCC GCGCCGCCTC 
TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT GAGCTCTACG AGAAGGTTTT CTCCAGGCGC 
GCGGACCGGC ACAGCGACTT CTCCCGCTTG GCGAGGGTGC TCACGGGGAA CACCATTGCC 
CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC TGCTCGCACA TCGGAGTACT AAAGGCATTA 
GAGGAGGCGG GGGTCCCCGT GGACCTGGTG GGCGGCACGT CCATTGGCTC TTTCATCGGA 
GCGTTGTACG CGGAGGAGCG CAGCGCCAGC CGCACGAAGC AGCGGGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCGGTGCT GGAACCTGTG TTGGACCTCA CGTACCCAGT CACCTCCATG 
TTCACTGGGT CTGCCTTTAA CCGCAGCATC CATCGGGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAACGTGACC ACAGATATCA CCGCCTCAGC CATGCGAGTC 
CACAAAGATG GCTCCCTGTG GCGGTACGTG CGCGCCAGCA TGACGCTGTC GGGCTACCTG 
CCCCCGCTGT GCGACCCCAA GGACGGGCAC CTACTCATGG ATGGCGGCTA CATCAACAAT 
CTGCCAGCGG ACATCGCCCG CAGCATGGGT GCCAAAACGG TCATCGCCAT TGACGTGGGG 
AGCCAGGATG AGACGGACCT CAGCACCTAC GGGGACAGCC TGTCCGGCTG GTGGCTGCTG 
TGGAAGCGGC TGAATCCCTG GGCTGACAAG GTAAAGGTTC CAGACATGGC TGAAATCCAG 
TCCCGCCTGG CCTACGTGTC CTGTGTGCGG CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC 
TGCGAGTACC TGCGCCCGCC CATCGACTGC TTCAAGACCA TGGACTTTGG GAAGTTCGAC 
CAGATCTATG ATGTGGGCTA CCAGTACGGG AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC 
AACGTCATTG AGAAAATGCT CACAGACCGG CGGTCTACAG ACCTTAATGA GAGCCGCCGT 
GCAGACGTGC TTGCCTTCCC AAGCTCTGGC TTCACTGACT TGGCAGAGAT TGTGTCCCGG 
ATTGAGCCCC CCACGAGCTA TGTCTCTGAT GGCTGTGCTG ACGGAGAGGA GTCAGATTGT 
CTGACAGAGT ATGAGGAGGA CGCCGGACCC GACTGCTCGA GGGATGAAGG GGGGTCCCCC 
GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA 
CGACGCTGTC TGCCCCAGGA GCCGCCCGGC TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159583.1
CDS	241..4368
Translation	MEAPLQTGMMGTSSHGLATNSSGAKVAERDGFQDVLAPGEGSAGRICGAQPVPFVPQVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDPVKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKILGNLQQLQGPFPAGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

Target ORF information:

RefSeq Version	NM_001166111.1
Organism	Homo sapiens(human)
Definition	Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166111.1

ORF Insert Sequence:

ATGGAGGCTC CGCTGCAAAC TGGAATGATG GGGACATCGA GTCACGGGCT GGCTACGAAC 
TCCTCGGGGG CGAAGGTGGC GGAGAGGGAT GGGTTCCAGG ACGTCCTGGC GCCCGGGGAA 
GGCTCGGCGG GACGGATTTG CGGTGCGCAG CCAGTGCCGT TCGTCCCTCA GGTGCTTGGC 
GTGATGATCG GGGCCGGAGT GGCGGTGGTG GTCACGGCCG TGCTCATCCT CCTGGTGGTG 
CGGAGGCTGC GAGTGCCAAA AACCCCAGCC CCGGATGGCC CCCGGTATCG GTTCCGGAAG 
AGGGACAAAG TGCTCTTCTA TGGCCGGAAG ATTATGCGGA AGGTGTCACA ATCCACCTCC 
TCCCTCGTGG ATACCTCTGT CTCCGCCACC TCCCGGCCAC GCATGAGGAA GAAACTGAAG 
ATGCTCAACA TTGCCAAGAA GATCCTGCGC ATCCAGAAAG AGACGCCCAC GCTGCAGCGG 
AAGGAGCCCC CGCCCGCAGT GCTAGAAGCT GACCTGACCG AGGGCGACCT GGCTAACTCC 
CATCTGCCCT CTGAAGTGCT TTATATGCTC AAGAACGTCC GGGTGCTGGG CCACTTCGAG 
AAGCCACTCT TCCTGGAGCT CTGCCGCCAC ATGGTCTTCC AGCGGCTGGG CCAGGGTGAC 
TACGTCTTCC GGCCGGGCCA GCCAGATGCC AGCATCTACG TGGTGCAGGA CGGGCTGCTG 
GAGCTCTGTC TGCCAGGGCC TGACGGGAAG GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG 
GACAGCGTCA ACAGCCTTCT CAGCATCCTG GATGTCATCA CCGGTCACCA GCATCCCCAG 
CGGACCGTGT CTGCCCGGGC GGCCCGGGAC TCCACGGTGC TGCGCCTGCC GGTGGAAGCA 
TTCTCCGCGG TCTTCACCAA GTACCCGGAG AGCTTGGTGC GGGTCGTGCA GATCATCATG 
GTGCGGCTGC AGCGAGTCAC CTTCCTGGCA CTGCACAACT ACCTGGGTCT GACCAATGAG 
CTCTTCAGCC ACGAGATCCA GCCCCTGCGT CTGTTCCCCA GCCCCGGCCT CCCAACTCGC 
ACCAGCCCTG TGCGGGGCTC CAAGAGAATG GTCAGCACCT CAGCTACAGA CGAGCCCAGG 
GAGACCCCAG GGCGGCCACC CGATCCCACC GGGGCCCCGC TGCCTGGACC TACAGGGGAC 
CCTGTGAAGC CCACATCCCT GGAAACCCCC TCGGCCCCTC TGCTGAGCCG CTGCGTCTCC 
ATGCCAGGGG ACATCTCAGG CTTGCAGGGT GGCCCCCGCT CCGACTTCGA CATGGCCTAT 
GAGCGTGGCC GGATCTCCGT GTCCCTGCAG GAAGAGGCCT CCGGGGGGTC CCTGGCAGCC 
CCCGCTCGGA CCCCCACTCA GGAGCCTCGT GAGCAGCCGG CAGGCGCCTG TGAATACAGC 
TACTGTGAGG ATGAGTCGGC CACTGGTGGC TGCCCTTTCG GGCCCTACCA GGGCCGCCAG 
ACCAGCAGCA TCTTCGAGGC AGCAAAGCAG GAGCTGGCCA AGCTGATGCG GATTGAGGAC 
CCCTCCCTCC TGAACAGCAG AGTCTTGCTG CACCACGCCA AAGCTGGCAC CATCATTGCC 
CGCCAGGGAG ACCAGGACGT GAGCCTGCAC TTCGTGCTCT GGGGCTGCCT GCACGTGTAC 
CAGCGCATGA TCGACAAGGC GGAGGACGTG TGCCTGTTCG TAGCGCAGCC CGGGGAACTG 
GTGGGGCAGC TGGCGGTGCT CACTGGCGAA CCTCTCATCT TCACACTGCG AGCCCAACGC 
GACTGCACCT TCCTGCGGAT CTCCAAGTCC GACTTCTATG AGATCATGCG CGCACAGCCC 
AGTGTGGTGC TGAGTGCGGC GCACACGGTG GCAGCCAGGA TGTCGCCCTT CGTGCGCCAG 
ATGGACTTCG CCATCGACTG GACTGCAGTG GAGGCGGGAC GCGCGCTGTA CAGGCAGGGC 
GACCGCTCCG ACTGCACTTA CATCGTGCTC AATGGGCGGC TGCGTAGCGT GATCCAGCGA 
GGCAGTGGCA AGAAGGAGCT GGTGGGCGAG TACGGCCGCG GCGACCTCAT CGGCGTGGTG 
GAGGCACTGA CCCGGCAGCC GCGAGCCACG ACGGTGCACG CGGTGCGCGA CACGGAGCTG 
GCCAAGCTTC CCGAGGGCAC CTTGGGTCAC ATCAAACGCC GGTACCCGCA GGTCGTGACC 
CGCCTTATCC ACCTACTGAG CCAGAAAATT CTAGGGAATT TGCAGCAGCT GCAAGGACCC 
TTCCCAGCAG GCTCTGGGTT GGGTGTGCCC CCACACTCGG AACTCACCAA CCCAGCCAGC 
AACCTGGCAA CTGTGGCAAT CCTGCCTGTG TGTGCTGAGG TCCCCATGGT GGCCTTCACG 
CTGGAGCTGC AGCACGCCCT GCAGGCCATC GGTCCGACGC TACTCCTTAA CAGTGACATC 
ATCCGGGCAC GCCTGGGGGC CTCCGCACTG GATAGCATCC AAGAGTTCCG GCTGTCAGGG 
TGGCTGGCCC AGCAGGAGGA TGCACACCGT ATCGTACTCT ACCAGACGGA CGCCTCGCTG 
ACGCCCTGGA CCGTGCGCTG CCTGCGACAG GCCGACTGCA TCCTCATTGT GGGCCTGGGG 
GACCAGGAGC CTACCCTCGG CCAGCTGGAG CAGATGCTGG AGAACACGGC TGTGCGCGCC 
CTTAAGCAGC TAGTCCTGCT CCACCGAGAG GAGGGCGCGG GCCCCACGCG CACCGTGGAG 
TGGCTAAATA TGCGCAGCTG GTGCTCGGGG CACCTGCACC TGCGCTGTCC GCGCCGCCTC 
TTTTCGCGCC GCAGCCCTGC CAAGCTGCAT GAGCTCTACG AGAAGGTTTT CTCCAGGCGC 
GCGGACCGGC ACAGCGACTT CTCCCGCTTG GCGAGGGTGC TCACGGGGAA CACCATTGCC 
CTTGTGCTAG GCGGGGGCGG GGCCAGGGGC TGCTCGCACA TCGGAGTACT AAAGGCATTA 
GAGGAGGCGG GGGTCCCCGT GGACCTGGTG GGCGGCACGT CCATTGGCTC TTTCATCGGA 
GCGTTGTACG CGGAGGAGCG CAGCGCCAGC CGCACGAAGC AGCGGGCCCG GGAGTGGGCC 
AAGAGCATGA CTTCGGTGCT GGAACCTGTG TTGGACCTCA CGTACCCAGT CACCTCCATG 
TTCACTGGGT CTGCCTTTAA CCGCAGCATC CATCGGGTCT TCCAGGATAA GCAGATTGAG 
GACCTGTGGC TGCCTTACTT CAACGTGACC ACAGATATCA CCGCCTCAGC CATGCGAGTC 
CACAAAGATG GCTCCCTGTG GCGGTACGTG CGCGCCAGCA TGACGCTGTC GGGCTACCTG 
CCCCCGCTGT GCGACCCCAA GGACGGGCAC CTACTCATGG ATGGCGGCTA CATCAACAAT 
CTGCCAGCGG ACATCGCCCG CAGCATGGGT GCCAAAACGG TCATCGCCAT TGACGTGGGG 
AGCCAGGATG AGACGGACCT CAGCACCTAC GGGGACAGCC TGTCCGGCTG GTGGCTGCTG 
TGGAAGCGGC TGAATCCCTG GGCTGACAAG GTAAAGGTTC CAGACATGGC TGAAATCCAG 
TCCCGCCTGG CCTACGTGTC CTGTGTGCGG CAGCTAGAGG TTGTCAAGTC CAGCTCCTAC 
TGCGAGTACC TGCGCCCGCC CATCGACTGC TTCAAGACCA TGGACTTTGG GAAGTTCGAC 
CAGATCTATG ATGTGGGCTA CCAGTACGGG AAGGCGGTGT TTGGAGGCTG GAGCCGTGGC 
AACGTCATTG AGAAAATGCT CACAGACCGG CGGTCTACAG ACCTTAATGA GAGCCGCCGT 
GCAGACGTGC TTGCCTTCCC AAGCTCTGGC TTCACTGACT TGGCAGAGAT TGTGTCCCGG 
ATTGAGCCCC CCACGAGCTA TGTCTCTGAT GGCTGTGCTG ACGGAGAGGA GTCAGATTGT 
CTGACAGAGT ATGAGGAGGA CGCCGGACCC GACTGCTCGA GGGATGAAGG GGGGTCCCCC 
GAGGGCGCAA GCCCCAGCAC TGCCTCCGAG ATGGAGGAGG AGAAGTCGAT TCTCCGGCAA 
CGACGCTGTC TGCCCCAGGA GCCGCCCGGC TCAGCCACAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Boucher Neuh?user Syndrome - A rare cause of inherited hypogonadotropic hypogonadism. A case of two adult siblings with two novel mutations in PNPLA6.
European journal of medical genetics60(2)105-109(2017 Feb)
Langdahl JH,Frederiksen AL,Nguyen N,Brusgaard K,Juhl CB

The PNPLA-family phospholipases involved in glycerophospholipid homeostasis of HeLa cells.
Biochimica et biophysica acta1861(9 Pt A)1058-1065(2016 09)
Hermansson M,H?nninen S,Hokynar K,Somerharju P

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PNPLA6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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