NCKAP1 cDNA ORF clone, Homo sapiens(human)

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu08058 NM_205842.3
Latest version!
Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu08140 NM_013436.5
Latest version!
Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu35768 XM_006712200.3
Latest version!
Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu35769 XM_006712201.3
Latest version!
Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu08058 NM_205842.2 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $797.30
$1139.00
OHu08140 NM_013436.4 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu08058
    Clone ID Related Accession (Same CDS sequence) NM_205842.2 , NM_205842.3
    Accession Version NM_205842.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product nck-associated protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, BC015025.1, CA314471.1 and AC108514.3. On May 31, 2019 this sequence version replaced NM_205842.2. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011159.1, SRR1803615.70352.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA
    GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA
    TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA
    GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT
    CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT
    TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT
    ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA
    CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG
    GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC
    AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC
    CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA
    CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA
    AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA
    ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT
    GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT
    AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT
    CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT
    GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA
    TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA
    ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG
    ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT
    CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT
    AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA
    TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995314.1
    CDS359..3763
    Translation

    Target ORF information:

    RefSeq Version NM_205842.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_205842.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA
    GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA
    TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA
    GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT
    CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT
    TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT
    ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA
    CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG
    GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC
    AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC
    CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA
    CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA
    AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA
    ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT
    GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT
    AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT
    CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT
    GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA
    TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA
    ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG
    ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT
    CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT
    AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA
    TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08140
    Clone ID Related Accession (Same CDS sequence) NM_013436.5 , NM_013436.4
    Accession Version NM_013436.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product nck-associated protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, BC015025.1, CA314471.1 and AC108514.3. On Nov 22, 2018 this sequence version replaced NM_013436.4. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.176887.1, SRR1803611.89941.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361354.9/ ENSP00000355348.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG
    AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT
    ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT
    ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC
    AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC
    AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA
    GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG
    TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG
    AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT
    TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT
    ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA
    GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA
    CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT
    GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT
    ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG
    AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT
    GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG
    TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA
    TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA
    AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT
    TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC
    TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG
    CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT
    ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA
    CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA
    GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA
    GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT
    GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT
    ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT
    GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA
    ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT
    ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA
    GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC
    AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA
    AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA
    GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA
    TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG
    AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT
    GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA
    CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA
    GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG
    TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT
    TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG
    GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT
    GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT
    GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG
    CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT
    AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG
    GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC
    AAACAAAGTG TTACATCTTC TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_038464.1
    CDS359..3745
    Translation

    Target ORF information:

    RefSeq Version NM_013436.5
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013436.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG
    AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT
    ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT
    ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC
    AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC
    AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA
    GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG
    TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG
    AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT
    TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT
    ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA
    GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA
    CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT
    GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT
    ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG
    AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT
    GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG
    TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA
    TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA
    AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT
    TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC
    TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG
    CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT
    ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA
    CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA
    GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA
    GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT
    GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT
    ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT
    GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA
    ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT
    ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA
    GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC
    AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA
    AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA
    GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA
    TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG
    AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT
    GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA
    CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA
    GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG
    TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT
    TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG
    GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT
    GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT
    GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG
    CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT
    AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG
    GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC
    AAACAAAGTG TTACATCTTC TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08140
    Clone ID Related Accession (Same CDS sequence) NM_013436.5 , NM_013436.4
    Accession Version NM_013436.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product nck-associated protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, AC064871.6, BC015025.1 and CA314471.1. On May 24, 2013 this sequence version replaced NM_013436.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015025.1, SRR1803611.108456.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG
    AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT
    ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT
    ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC
    AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC
    AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA
    GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG
    TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG
    AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT
    TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT
    ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA
    GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA
    CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT
    GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT
    ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG
    AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT
    GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG
    TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA
    TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA
    AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT
    TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC
    TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG
    CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT
    ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA
    CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA
    GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA
    GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT
    GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT
    ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT
    GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA
    ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT
    ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA
    GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC
    AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA
    AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA
    GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA
    TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG
    AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT
    GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA
    CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA
    GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG
    TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT
    TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG
    GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT
    GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT
    GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG
    CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT
    AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG
    GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC
    AAACAAAGTG TTACATCTTC TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_038464.1
    CDS760..4146
    Translation

    Target ORF information:

    RefSeq Version NM_013436.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013436.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG
    AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT
    ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC
    TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT
    ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC
    AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC
    AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA
    GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT
    AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG
    TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG
    AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT
    TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT
    ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA
    GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA
    CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT
    GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT
    ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG
    AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT
    GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG
    TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA
    TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA
    AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT
    TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC
    TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG
    CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT
    ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA
    CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA
    GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA
    GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT
    GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT
    ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT
    GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA
    ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT
    ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA
    GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC
    AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA
    AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA
    GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA
    TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG
    AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT
    GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA
    CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA
    GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG
    TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT
    TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG
    GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT
    GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT
    GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG
    CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT
    AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG
    GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC
    AAACAAAGTG TTACATCTTC TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08058
    Clone ID Related Accession (Same CDS sequence) NM_205842.2 , NM_205842.3
    Accession Version NM_205842.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product nck-associated protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, AC064871.6, BC015025.1 and CA314471.1. On May 24, 2013 this sequence version replaced NM_205842.1. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011159.1, SRR1660809.212111.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA
    GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA
    TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA
    GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT
    CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT
    TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT
    ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA
    CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG
    GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC
    AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC
    CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA
    CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA
    AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA
    ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT
    GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT
    AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT
    CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT
    GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA
    TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA
    ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG
    ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT
    CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT
    AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA
    TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_995314.1
    CDS760..4164
    Translation

    Target ORF information:

    RefSeq Version NM_205842.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_205842.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA
    CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA
    GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA
    TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA
    GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT
    CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT
    GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT
    TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA
    CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT
    ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA
    CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG
    GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC
    AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC
    CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA
    CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA
    AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA
    ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT
    GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT
    AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT
    CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT
    GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA
    TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA
    ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG
    ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT
    CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT
    AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA
    TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35768
    Clone ID Related Accession (Same CDS sequence) XM_006712200.3
    Accession Version XM_006712200.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product nck-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT
    GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA
    TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT
    TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA
    ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC
    ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA
    CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT
    GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA
    AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCACCT
    AGTACAATGC TTAATCCAGC ACAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT
    GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC
    GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT
    CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC
    TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT
    TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC
    CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC
    TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT
    GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA
    GCACATGTGA GGAAATACGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC
    TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA
    TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA
    GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT
    ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG
    AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA
    TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT
    TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT
    ATGAGTTGCA CGCATGAACT ATGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT
    TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT
    ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTAC CCAAGCATTG TGCCAAAACT
    ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA
    AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT
    AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG
    GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT
    ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT
    TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT
    GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA
    GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG
    TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC GTTTGTGAAC
    TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG
    AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG
    TGGCATATTT CATCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG
    TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT GTTTAAAAGA
    TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC
    CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA
    AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG
    AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC
    GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC
    TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT
    CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC
    AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA
    GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT
    ACAACAAGAA ATAGAGAATC TGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA
    TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC
    CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712263.1
    CDS252..3650
    Translation

    Target ORF information:

    RefSeq Version XM_006712200.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712200.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT
    GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA
    TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT
    TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA
    ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC
    ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA
    CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT
    GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA
    AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCACCT
    AGTACAATGC TTAATCCAGC ACAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT
    GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC
    GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT
    CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC
    TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT
    TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC
    CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC
    TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT
    GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA
    GCACATGTGA GGAAATACGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC
    TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA
    TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA
    GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT
    ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG
    AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA
    TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT
    TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT
    ATGAGTTGCA CGCATGAACT ATGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT
    TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT
    ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTAC CCAAGCATTG TGCCAAAACT
    ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA
    AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT
    AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG
    GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT
    ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT
    TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT
    GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA
    GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG
    TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC GTTTGTGAAC
    TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG
    AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG
    TGGCATATTT CATCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG
    TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT GTTTAAAAGA
    TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC
    CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA
    AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG
    AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC
    GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC
    TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT
    CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC
    AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA
    GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT
    ACAACAAGAA ATAGAGAATC TGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA
    TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC
    CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35769
    Clone ID Related Accession (Same CDS sequence) XM_006712201.3
    Accession Version XM_006712201.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product nck-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA
    AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA
    TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT
    ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA
    CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT
    GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT
    GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT
    CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC
    CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAATCCA
    GCACAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT
    ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT
    TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC
    ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT
    GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA
    CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA
    TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC
    ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT
    AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAC
    GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC
    AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT
    TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT
    TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT
    TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT
    ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT
    TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA
    TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACGCATGAA
    CTATGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC
    CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT
    ACCCTTAGTG ACCAGTTGCT ACCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT
    AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT
    GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT
    TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT
    ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG
    ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT
    GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA
    AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA
    ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT
    GGCCATATAG CATATTTTCC TGCAATGAAA GCGTTTGTGA ACTTACCTAC AGAAAATGAA
    TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA
    CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCATCACAA
    GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC
    AGCTTTGACA AACCAGACCA GATGGCTGCA CTGTTTAAAA GATTATCATC TGTTGACAGT
    GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA
    GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT
    AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA
    TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA
    AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT
    GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA
    GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT
    TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA
    TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA
    TCTGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT
    TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA
    AGTGTTACAT CTTCTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712264.1
    CDS254..3634
    Translation

    Target ORF information:

    RefSeq Version XM_006712201.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712201.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC
    CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA
    AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA
    TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT
    ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA
    CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT
    GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT
    GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT
    CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC
    CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAATCCA
    GCACAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT
    ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT
    TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC
    ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT
    GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA
    CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA
    TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC
    ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT
    AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAC
    GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC
    AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT
    TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT
    TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT
    TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT
    ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT
    TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA
    TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACGCATGAA
    CTATGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC
    CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT
    ACCCTTAGTG ACCAGTTGCT ACCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT
    AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT
    GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT
    TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT
    ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG
    ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT
    GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA
    AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA
    ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT
    GGCCATATAG CATATTTTCC TGCAATGAAA GCGTTTGTGA ACTTACCTAC AGAAAATGAA
    TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA
    CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCATCACAA
    GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC
    AGCTTTGACA AACCAGACCA GATGGCTGCA CTGTTTAAAA GATTATCATC TGTTGACAGT
    GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA
    GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT
    AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA
    TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA
    AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT
    GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA
    GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT
    TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA
    TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA
    TCTGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT
    TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA
    AGTGTTACAT CTTCTGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.