NCKAP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCKAP1
Entrez Gene ID	10787
Full Name	NCK associated protein 1
Synonyms	HEM2,NAP1,NAP125,p125Nap1
General protein information	Preferred Names NCK associated protein 1 Names nck-associated protein 1 membrane-associated protein HEM-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q32.1
Disorder MIM:	604891

mRNA and Protein(s)

mRNA	Protein	Name
NM_205842.2	NP_995314.1	nck-associated protein 1 isoform 2
XM_006712201.3	XP_006712264.1	nck-associated protein 1 isoform X2
NM_013436.5	NP_038464.1	nck-associated protein 1 isoform 1
XM_006712200.3	XP_006712263.1	nck-associated protein 1 isoform X1
NM_205842.3	NP_995314.1	nck-associated protein 1 isoform 2
NM_013436.4	NP_038464.1	nck-associated protein 1 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NCKAP1	XP_535990.3
Xenopus tropicalis (tropical clawed frog)	nckap1	XP_004917966.1
Pan troglodytes (chimpanzee)	NCKAP1	XP_001148485.2
Rattus norvegicus (Norway rat)	Nckap1	NP_113806.1
Drosophila melanogaster (fruit fly)	Hem	NP_524214.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004946	XP_315044.2
	NCKAP1	XP_001099879.2
Bos taurus (cattle)	LOC101907541	XP_005202363.1
Mus musculus (house mouse)	Nckap1	NP_058661.1
Arabidopsis thaliana (thale cress)	GRL	NP_001031485.1
Oryza sativa (rice)	Os08g0544500	NP_001062406.2
Homo sapiens (human)	NCKAP1	NP_995314.1
Gallus gallus (chicken)	NCKAP1	XP_001232275.2
Danio rerio (zebrafish)	nckap1	NP_001131137.1
Caenorhabditis elegans (roundworm)	gex-3	NP_502368.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-34c-3p promotes cell proliferation and invasion in hepatocellular carcinoma by regulation of NCKAP1 expression.
Xiao CZ, Wei W, Guo ZX, Zhang MY, Zhang YF, Wang JH, Shi M, Wang HY, Guo RP
Journal of cancer research and clinical oncology143(2)263-273(2017 Feb)

The Contribution of Mosaic Variants to Autism Spectrum Disorder.
Freed D, Pevsner J
PLoS genetics12(9)e1006245(2016 Sep)

The WASF3-NCKAP1-CYFIP1 Complex Is Essential for Breast Cancer Metastasis.
Teng Y, Qin H, Bahassan A, Bendzunas NG, Kennedy EJ, Cowell JK
Cancer research76(17)5133-42(2016 Sep)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-34c-3p functions as a tumor promoter by targeting NCKAP1 that is associated with prognosis in hepatocellular carcinoma.
Title: MicroRNA-34c-3p promotes cell proliferation and invasion in hepatocellular carcinoma by regulation of NCKAP1 expression.

We identify three mosaic missense and likely-gene disrupting mutations in genes previously implicated in ASD (KMT2C, NCKAP1, and MYH10) in probands but none in siblings. We find a strong ascertainment bias for mosaic mutations in probands relative to their unaffected siblings
Title: The Contribution of Mosaic Variants to Autism Spectrum Disorder.

we show that stapled peptides targeting the interface between NCKAP1 and CYFIP1 destabilize the WASF3 complex... thereby suppressing invasion and metastasis of breast cancer cells.
Title: The WASF3-NCKAP1-CYFIP1 Complex Is Essential for Breast Cancer Metastasis.

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08058	NM_205842.3 Latest version!	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu08140	NM_013436.5 Latest version!	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu35768	XM_006712200.3 Latest version!	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu35769	XM_006712201.3 Latest version!	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu08058	NM_205842.2	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$797.30 $1139.00
OHu08140	NM_013436.4	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCKAP1 ( NM_205842.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_995314.1 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08058
Clone ID Related Accession (Same CDS sequence)	NM_205842.2 , NM_205842.3
Accession Version	NM_205842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	nck-associated protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, BC015025.1, CA314471.1 and AC108514.3. On May 31, 2019 this sequence version replaced NM_205842.2. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011159.1, SRR1803615.70352.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995314.1
CDS	359..3763
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_205842.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205842.3

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( NM_013436.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_038464.1 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08140
Clone ID Related Accession (Same CDS sequence)	NM_013436.5 , NM_013436.4
Accession Version	NM_013436.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	nck-associated protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, BC015025.1, CA314471.1 and AC108514.3. On Nov 22, 2018 this sequence version replaced NM_013436.4. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.176887.1, SRR1803611.89941.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361354.9/ ENSP00000355348.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT 
AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG 
CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038464.1
CDS	359..3745
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_013436.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013436.5

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT 
AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG 
CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( NM_013436.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_038464.1 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

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CloneID	OHu08140
Clone ID Related Accession (Same CDS sequence)	NM_013436.5 , NM_013436.4
Accession Version	NM_013436.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	nck-associated protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, AC064871.6, BC015025.1 and CA314471.1. On May 24, 2013 this sequence version replaced NM_013436.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015025.1, SRR1803611.108456.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT 
AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG 
CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038464.1
CDS	760..4146
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_013436.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013436.4

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC AACAGCTTGC ACAACTACAG 
AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT 
ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGACGTTTG CCAAGTCTTC 
TTTGATATTA CTGTAAACTT TGATTTAACA AAGAACTACT TAGATTTAAT TATAACCTAT 
ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC 
AACTATGCCC ATGAAATGAC TCATGGAGCA AGTGACAGAG AATACCCACG CCTTGGCCAG 
ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAGATGATGG AAGAATTTGT ACCCCATAGC 
AAGTCTCTTT CAGATGCACT AATTTCTCTT CAAATGGTAT ATCCTCGAAG GAATCTTTCA 
GCTGACCAGT GGAGAAATGC CCAGTTATTG AGCCTCATCA GTGCACCTAG TACAATGCTT 
AATCCAGCAC AGTCCGACAC TATGCCTTGT GAATACCTCT CTTTGGATGC AATGGAAAAG 
TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGACGC TACAGCACTG 
AACCTTTGGA AACTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTCTTTCG GGATGAAGTT 
TTCCACATTC ACAAAGCTGC AGAAGACTTA TTTGTAAACA TACGAGGCTA TAATAAACGT 
ATTAATGACA TAAGAGAATG CAAGGAGGCA GCCGTGTCAC ATGCTGGTTC AATGCACAGA 
GAAAGACGCA AGTTTTTAAG ATCTGCACTG AAGGAATTGG CTACTGTCCT CTCTGATCAA 
CCTGGATTGC TAGGTCCCAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT 
GAAATCATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGACGACTTT 
ATAGATAAGC ACATTGCTGA ATTAATATTT TACATGGAAG AACTTAGAGC ACATGTGAGG 
AAATACGGAC CTGTAATGCA GAGGTATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT 
GTCCTCAATG AACTCGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG 
TCCTCTTTTG TTAACACTAT GACTTCCCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA 
TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTAGGTTAC AGGCATATAC TAGTGTCTCA 
AAGGCTTCAC TTGGCCTTGC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT 
TTTCATACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC 
ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC 
TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACG 
CATGAACTAT GTCCAGAAGA GCGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT 
ATGTTCCTAG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA 
CAGTGTACCC TTAGTGACCA GTTGCTACCC AAGCATTGTG CCAAAACTAT CAGTCAAGCA 
GTGAATAAGA AATCAAAAAA GCAGACTGGT AAGAAAGGGG AACCTGAAAG GGAGAAACCA 
GGTGTTGAGA GCATGAGGAA AAACAGGCTG GTTGTGACCA ACCTTGATAA ATTGCACACT 
GCACTTTCTG AGTTATGCTT CTCTATAAAT TATGTACCAA ACATGGTGGT ATGGGAACAT 
ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TACGCTTTAC CAAGTCAATT 
GTTGGGATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCTTC AGAACTTCTA 
ACAAGTGTAA GAGCATACAT GACCGTACTC CAGTCAATAG AAAACTATGT GCAGATTGAT 
ATTACAAGAG TATTTAATAA TGTGCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA 
GAGCCAACCA TTACAAGTCT ATACACAAAT TGGTATTTGG AAACTTTGTT ACGACAAGTC 
AGCAATGGCC ATATAGCATA TTTTCCTGCA ATGAAAGCGT TTGTGAACTT ACCTACAGAA 
AATGAATTAA CATTCAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA 
GAACTACTAG GCCCATATGG TATGAAGTTT CTAAGTGAAA GCCTTATGTG GCATATTTCA 
TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGTT AACACAAATG 
AGGACCAGCT TTGACAAACC AGACCAGATG GCTGCACTGT TTAAAAGATT ATCATCTGTT 
GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCCTTCCG ATCATTGGCA 
CAAGAAGCAC TTAGAGATGT CTTATCCTAC CACATTCCTT TTCTTGTAAG TTCAATTGAA 
GATTTTAAGG ATCACATTCC AAGGGAAACT GATATGAAGG TTGCAATGAA TGTGTATGAG 
TTATCATCAG CTGCCGGATT ACCTTGTGAG ATTGATCCTG CATTGGTCGT AGCTCTTTCT 
TCACAAAAAT CGGAAAACAT TAGTCCAGAA GAAGAGTATA AAATTGCCTG CCTTCTCATG 
GTGTTTGTGG CAGTTTCTTT GCCAACACTG GCCAGTAATG TGATGTCTCA GTACAGCCCT 
GCTATAGAAG GGCATTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATTGCT 
GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATTGAAGACC GTCTTAAAGA ATTTCTGGCG 
CTTGCATCCT CCAGTCTACT GAAAATTGGC CAGGAGACAG ATAAAACTAC AACAAGAAAT 
AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTACAAG AATCTCCATT CCTTACAATG 
GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC 
AAACAAAGTG TTACATCTTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( NM_205842.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_995314.1 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.

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CloneID	OHu08058
Clone ID Related Accession (Same CDS sequence)	NM_205842.2 , NM_205842.3
Accession Version	NM_205842.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	nck-associated protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011159.1, AC064871.6, BC015025.1 and CA314471.1. On May 24, 2013 this sequence version replaced NM_205842.1. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011159.1, SRR1660809.212111.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995314.1
CDS	760..4164
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	NM_205842.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205842.2

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA 
CAGCTTGCAC AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC 
TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT 
GACGTTTGCC AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA 
GATTTAATTA TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG 
GCAATCATTG GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA 
TACCCACGCC TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA 
GAATTTGTAC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT 
CCTCGAAGGA ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT 
GCACCTAGTA CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT 
TTGGATGCAA TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT 
ACTGACGCTA CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT 
CTCTTTCGGG ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA 
CGAGGCTATA ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT 
GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT 
ACTGTCCTCT CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA 
TTATCCTTTG CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG 
AAGAGTGCAG ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA 
CTTAGAGCAC ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT 
TCTGGCTTTG ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA 
GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA 
GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG 
GCATATACTA GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG 
ATGATGAATA CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG 
GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA 
CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT 
CATTTTATGA GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC 
AGTCTTTCCT TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC 
ACTGATATTT GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC 
AAAACTATCA GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA 
CCTGAAAGGG AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC 
CTTGATAAAT TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC 
ATGGTGGTAT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA 
CGCTTTACCA AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA 
AAACCTTCAG AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA 
AACTATGTGC AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA 
CATTTAGACA GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA 
ACTTTGTTAC GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT 
GTGAACTTAC CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA 
GAAATGAGGT CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC 
CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT 
GATGTGTTAA CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT 
AAAAGATTAT CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA 
TCCTTCCGAT CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT 
CTTGTAAGTT CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT 
GCAATGAATG TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA 
TTGGTCGTAG CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA 
ATTGCCTGCC TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG 
ATGTCTCAGT ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA 
GCCATCAACC AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT 
CTTAAAGAAT TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT 
AAAACTACAA CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA 
TCTCCATTCC TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT 
GCATACCATG CTGTCTACAA ACAAAGTGTT ACATCTTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_006712200.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712263.1 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

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CloneID	OHu35768
Clone ID Related Accession (Same CDS sequence)	XM_006712200.3
Accession Version	XM_006712200.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT 
GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA 
ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC 
ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA 
CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA 
AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCACCT 
AGTACAATGC TTAATCCAGC ACAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT 
GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC 
GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC 
TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT 
TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC 
CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC 
TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT 
GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA 
GCACATGTGA GGAAATACGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA 
TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA 
GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT 
ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA 
TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT 
TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT 
ATGAGTTGCA CGCATGAACT ATGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT 
TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT 
ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTAC CCAAGCATTG TGCCAAAACT 
ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA 
AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT 
AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG 
GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT 
ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT 
TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT 
GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA 
GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG 
TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC GTTTGTGAAC 
TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG 
AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG 
TGGCATATTT CATCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG 
TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT GTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC 
CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA 
AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC 
GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC 
TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT 
CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA 
GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC TGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA 
TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712263.1
CDS	252..3650
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_006712200.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712200.3

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT 
TGGAAGGCAT GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA 
TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCTT 
GCACAACTAC AGAAAGAAAA ATCAGAGATT CTGAAAAATC TGGCATTATA TTACTTCACA 
TTTGTAGATG TTATGGAATT TAAGGACCAT GTTTGTGAAT TGCTGAATAC TATTGACGTT 
TGCCAAGTCT TCTTTGATAT TACTGTAAAC TTTGATTTAA CAAAGAACTA CTTAGATTTA 
ATTATAACCT ATACAACACT AATGATACTG CTGTCTCGAA TTGAAGAAAG GAAGGCAATC 
ATTGGATTAT ACAACTATGC CCATGAAATG ACTCATGGAG CAAGTGACAG AGAATACCCA 
CGCCTTGGCC AGATGATTGT GGATTATGAA AACCCTTTAA AGAAGATGAT GGAAGAATTT 
GTACCCCATA GCAAGTCTCT TTCAGATGCA CTAATTTCTC TTCAAATGGT ATATCCTCGA 
AGGAATCTTT CAGCTGACCA GTGGAGAAAT GCCCAGTTAT TGAGCCTCAT CAGTGCACCT 
AGTACAATGC TTAATCCAGC ACAGTCCGAC ACTATGCCTT GTGAATACCT CTCTTTGGAT 
GCAATGGAAA AGTGGATTAT CTTTGGCTTT ATTTTGTGCC ATGGGATCCT AAATACTGAC 
GCTACAGCAC TGAACCTTTG GAAACTAGCT CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT 
CGGGATGAAG TTTTCCACAT TCACAAAGCT GCAGAAGACT TATTTGTAAA CATACGAGGC 
TATAATAAAC GTATTAATGA CATAAGAGAA TGCAAGGAGG CAGCCGTGTC ACATGCTGGT 
TCAATGCACA GAGAAAGACG CAAGTTTTTA AGATCTGCAC TGAAGGAATT GGCTACTGTC 
CTCTCTGATC AACCTGGATT GCTAGGTCCC AAGGCACTTT TTGTTTTTAT GGCATTATCC 
TTTGCCCGTG ATGAAATCAT CTGGCTACTT CGTCATGCAG ATAACATGCC AAAGAAGAGT 
GCAGACGACT TTATAGATAA GCACATTGCT GAATTAATAT TTTACATGGA AGAACTTAGA 
GCACATGTGA GGAAATACGG ACCTGTAATG CAGAGGTATT ACGTGCAGTA CCTTTCTGGC 
TTTGATGCTG TTGTCCTCAA TGAACTCGTG CAGAATCTTT CTGTTTGCCC TGAAGATGAA 
TCAATCATCA TGTCCTCTTT TGTTAACACT ATGACTTCCC TAAGTGTAAA ACAAGTTGAA 
GATGGGGAAG TATTTGATTT CAGAGGAATG AGATTAGATT GGTTTAGGTT ACAGGCATAT 
ACTAGTGTCT CAAAGGCTTC ACTTGGCCTT GCAGATCACA GAGAACTTGG AAAGATGATG 
AATACAATAA TTTTTCATAC AAAAATGGTA GATTCCTTGG TGGAAATGTT GGTGGAAACA 
TCAGATCTCT CCATATTTTG TTTTTATAGT CGTGCTTTTG AGAAGATGTT TCAACAGTGT 
TTGGAGTTAC CCTCTCAATC AAGATACTCA ATTGCATTTC CACTACTTTG CACTCATTTT 
ATGAGTTGCA CGCATGAACT ATGTCCAGAA GAGCGACATC ATATTGGAGA TCGCAGTCTT 
TCCTTATGTA ATATGTTCCT AGATGAAATG GCCAAACAAG CTCGAAATCT CATCACTGAT 
ATTTGCACAG AACAGTGTAC CCTTAGTGAC CAGTTGCTAC CCAAGCATTG TGCCAAAACT 
ATCAGTCAAG CAGTGAATAA GAAATCAAAA AAGCAGACTG GTAAGAAAGG GGAACCTGAA 
AGGGAGAAAC CAGGTGTTGA GAGCATGAGG AAAAACAGGC TGGTTGTGAC CAACCTTGAT 
AAATTGCACA CTGCACTTTC TGAGTTATGC TTCTCTATAA ATTATGTACC AAACATGGTG 
GTATGGGAAC ATACCTTTAC CCCACGAGAA TATTTGACTT CTCATCTGGA AATACGCTTT 
ACCAAGTCAA TTGTTGGGAT GACTATGTAT AATCAAGCCA CACAGGAAAT TGCAAAACCT 
TCAGAACTTC TAACAAGTGT AAGAGCATAC ATGACCGTAC TCCAGTCAAT AGAAAACTAT 
GTGCAGATTG ATATTACAAG AGTATTTAAT AATGTGCTTC TTCAACAAAC ACAACATTTA 
GACAGTCATG GAGAGCCAAC CATTACAAGT CTATACACAA ATTGGTATTT GGAAACTTTG 
TTACGACAAG TCAGCAATGG CCATATAGCA TATTTTCCTG CAATGAAAGC GTTTGTGAAC 
TTACCTACAG AAAATGAATT AACATTCAAT GCAGAGGAAT ATTCTGACAT ATCAGAAATG 
AGGTCATTAT CAGAACTACT AGGCCCATAT GGTATGAAGT TTCTAAGTGA AAGCCTTATG 
TGGCATATTT CATCACAAGT TGCTGAACTT AAGAAACTTG TGGTGGAGAA TGTTGATGTG 
TTAACACAAA TGAGGACCAG CTTTGACAAA CCAGACCAGA TGGCTGCACT GTTTAAAAGA 
TTATCATCTG TTGACAGTGT CTTGAAGAGG ATGACAATAA TTGGTGTAAT TTTATCCTTC 
CGATCATTGG CACAAGAAGC ACTTAGAGAT GTCTTATCCT ACCACATTCC TTTTCTTGTA 
AGTTCAATTG AAGATTTTAA GGATCACATT CCAAGGGAAA CTGATATGAA GGTTGCAATG 
AATGTGTATG AGTTATCATC AGCTGCCGGA TTACCTTGTG AGATTGATCC TGCATTGGTC 
GTAGCTCTTT CTTCACAAAA ATCGGAAAAC ATTAGTCCAG AAGAAGAGTA TAAAATTGCC 
TGCCTTCTCA TGGTGTTTGT GGCAGTTTCT TTGCCAACAC TGGCCAGTAA TGTGATGTCT 
CAGTACAGCC CTGCTATAGA AGGGCATTGC AACAACATAC ATTGCTTGGC CAAAGCCATC 
AACCAGATTG CTGCAGCTTT GTTTACAATT CACAAAGGAA GCATTGAAGA CCGTCTTAAA 
GAATTTCTGG CGCTTGCATC CTCCAGTCTA CTGAAAATTG GCCAGGAGAC AGATAAAACT 
ACAACAAGAA ATAGAGAATC TGTTTATTTA CTGCTAGATA TGATTGTACA AGAATCTCCA 
TTCCTTACAA TGGATCTTTT GGAATCTTGT TTTCCTTATG TCTTGCTGAG AAATGCATAC 
CATGCTGTCT ACAAACAAAG TGTTACATCT TCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_006712201.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712264.1 Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

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CloneID	OHu35769
Clone ID Related Accession (Same CDS sequence)	XM_006712201.3
Accession Version	XM_006712201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA 
CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT 
GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT 
GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC 
CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAATCCA 
GCACAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT 
ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT 
TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT 
GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA 
TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC 
ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT 
AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAC 
GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT 
TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT 
TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT 
TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT 
ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT 
TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA 
TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACGCATGAA 
CTATGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC 
CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT 
ACCCTTAGTG ACCAGTTGCT ACCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT 
AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT 
GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT 
TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT 
ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG 
ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT 
GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA 
AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA 
ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT 
GGCCATATAG CATATTTTCC TGCAATGAAA GCGTTTGTGA ACTTACCTAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA 
CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCATCACAA 
GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTGTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA 
GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT 
AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA 
TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA 
AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT 
GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA 
GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA 
TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCTGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT 
TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712264.1
CDS	254..3634
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_006712201.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712201.3

ORF Insert Sequence:

ATGTCGCGCT CAGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAGGCAA AACCATCCTA TCTTATCGAC AAAAACCTGG AATCTGCTGT GAAATTCATA 
GTCAGAAAAT TCCCTGCTGT AGAAACCCGC AACAACAATC TTGCACAACT ACAGAAAGAA 
AAATCAGAGA TTCTGAAAAA TCTGGCATTA TATTACTTCA CATTTGTAGA TGTTATGGAA 
TTTAAGGACC ATGTTTGTGA ATTGCTGAAT ACTATTGACG TTTGCCAAGT CTTCTTTGAT 
ATTACTGTAA ACTTTGATTT AACAAAGAAC TACTTAGATT TAATTATAAC CTATACAACA 
CTAATGATAC TGCTGTCTCG AATTGAAGAA AGGAAGGCAA TCATTGGATT ATACAACTAT 
GCCCATGAAA TGACTCATGG AGCAAGTGAC AGAGAATACC CACGCCTTGG CCAGATGATT 
GTGGATTATG AAAACCCTTT AAAGAAGATG ATGGAAGAAT TTGTACCCCA TAGCAAGTCT 
CTTTCAGATG CACTAATTTC TCTTCAAATG GTATATCCTC GAAGGAATCT TTCAGCTGAC 
CAGTGGAGAA ATGCCCAGTT ATTGAGCCTC ATCAGTGCAC CTAGTACAAT GCTTAATCCA 
GCACAGTCCG ACACTATGCC TTGTGAATAC CTCTCTTTGG ATGCAATGGA AAAGTGGATT 
ATCTTTGGCT TTATTTTGTG CCATGGGATC CTAAATACTG ACGCTACAGC ACTGAACCTT 
TGGAAACTAG CTCTTCAAAG TAGCTCTTGC CTCTCTCTCT TTCGGGATGA AGTTTTCCAC 
ATTCACAAAG CTGCAGAAGA CTTATTTGTA AACATACGAG GCTATAATAA ACGTATTAAT 
GACATAAGAG AATGCAAGGA GGCAGCCGTG TCACATGCTG GTTCAATGCA CAGAGAAAGA 
CGCAAGTTTT TAAGATCTGC ACTGAAGGAA TTGGCTACTG TCCTCTCTGA TCAACCTGGA 
TTGCTAGGTC CCAAGGCACT TTTTGTTTTT ATGGCATTAT CCTTTGCCCG TGATGAAATC 
ATCTGGCTAC TTCGTCATGC AGATAACATG CCAAAGAAGA GTGCAGACGA CTTTATAGAT 
AAGCACATTG CTGAATTAAT ATTTTACATG GAAGAACTTA GAGCACATGT GAGGAAATAC 
GGACCTGTAA TGCAGAGGTA TTACGTGCAG TACCTTTCTG GCTTTGATGC TGTTGTCCTC 
AATGAACTCG TGCAGAATCT TTCTGTTTGC CCTGAAGATG AATCAATCAT CATGTCCTCT 
TTTGTTAACA CTATGACTTC CCTAAGTGTA AAACAAGTTG AAGATGGGGA AGTATTTGAT 
TTCAGAGGAA TGAGATTAGA TTGGTTTAGG TTACAGGCAT ATACTAGTGT CTCAAAGGCT 
TCACTTGGCC TTGCAGATCA CAGAGAACTT GGAAAGATGA TGAATACAAT AATTTTTCAT 
ACAAAAATGG TAGATTCCTT GGTGGAAATG TTGGTGGAAA CATCAGATCT CTCCATATTT 
TGTTTTTATA GTCGTGCTTT TGAGAAGATG TTTCAACAGT GTTTGGAGTT ACCCTCTCAA 
TCAAGATACT CAATTGCATT TCCACTACTT TGCACTCATT TTATGAGTTG CACGCATGAA 
CTATGTCCAG AAGAGCGACA TCATATTGGA GATCGCAGTC TTTCCTTATG TAATATGTTC 
CTAGATGAAA TGGCCAAACA AGCTCGAAAT CTCATCACTG ATATTTGCAC AGAACAGTGT 
ACCCTTAGTG ACCAGTTGCT ACCCAAGCAT TGTGCCAAAA CTATCAGTCA AGCAGTGAAT 
AAGAAATCAA AAAAGCAGAC TGGTAAGAAA GGGGAACCTG AAAGGGAGAA ACCAGGTGTT 
GAGAGCATGA GGAAAAACAG GCTGGTTGTG ACCAACCTTG ATAAATTGCA CACTGCACTT 
TCTGAGTTAT GCTTCTCTAT AAATTATGTA CCAAACATGG TGGTATGGGA ACATACCTTT 
ACCCCACGAG AATATTTGAC TTCTCATCTG GAAATACGCT TTACCAAGTC AATTGTTGGG 
ATGACTATGT ATAATCAAGC CACACAGGAA ATTGCAAAAC CTTCAGAACT TCTAACAAGT 
GTAAGAGCAT ACATGACCGT ACTCCAGTCA ATAGAAAACT ATGTGCAGAT TGATATTACA 
AGAGTATTTA ATAATGTGCT TCTTCAACAA ACACAACATT TAGACAGTCA TGGAGAGCCA 
ACCATTACAA GTCTATACAC AAATTGGTAT TTGGAAACTT TGTTACGACA AGTCAGCAAT 
GGCCATATAG CATATTTTCC TGCAATGAAA GCGTTTGTGA ACTTACCTAC AGAAAATGAA 
TTAACATTCA ATGCAGAGGA ATATTCTGAC ATATCAGAAA TGAGGTCATT ATCAGAACTA 
CTAGGCCCAT ATGGTATGAA GTTTCTAAGT GAAAGCCTTA TGTGGCATAT TTCATCACAA 
GTTGCTGAAC TTAAGAAACT TGTGGTGGAG AATGTTGATG TGTTAACACA AATGAGGACC 
AGCTTTGACA AACCAGACCA GATGGCTGCA CTGTTTAAAA GATTATCATC TGTTGACAGT 
GTCTTGAAGA GGATGACAAT AATTGGTGTA ATTTTATCCT TCCGATCATT GGCACAAGAA 
GCACTTAGAG ATGTCTTATC CTACCACATT CCTTTTCTTG TAAGTTCAAT TGAAGATTTT 
AAGGATCACA TTCCAAGGGA AACTGATATG AAGGTTGCAA TGAATGTGTA TGAGTTATCA 
TCAGCTGCCG GATTACCTTG TGAGATTGAT CCTGCATTGG TCGTAGCTCT TTCTTCACAA 
AAATCGGAAA ACATTAGTCC AGAAGAAGAG TATAAAATTG CCTGCCTTCT CATGGTGTTT 
GTGGCAGTTT CTTTGCCAAC ACTGGCCAGT AATGTGATGT CTCAGTACAG CCCTGCTATA 
GAAGGGCATT GCAACAACAT ACATTGCTTG GCCAAAGCCA TCAACCAGAT TGCTGCAGCT 
TTGTTTACAA TTCACAAAGG AAGCATTGAA GACCGTCTTA AAGAATTTCT GGCGCTTGCA 
TCCTCCAGTC TACTGAAAAT TGGCCAGGAG ACAGATAAAA CTACAACAAG AAATAGAGAA 
TCTGTTTATT TACTGCTAGA TATGATTGTA CAAGAATCTC CATTCCTTAC AATGGATCTT 
TTGGAATCTT GTTTTCCTTA TGTCTTGCTG AGAAATGCAT ACCATGCTGT CTACAAACAA 
AGTGTTACAT CTTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-34c-3p promotes cell proliferation and invasion in hepatocellular carcinoma by regulation of NCKAP1 expression.
Journal of cancer research and clinical oncology143(2)263-273(2017 Feb)
Xiao CZ,Wei W,Guo ZX,Zhang MY,Zhang YF,Wang JH,Shi M,Wang HY,Guo RP

The Contribution of Mosaic Variants to Autism Spectrum Disorder.
PLoS genetics12(9)e1006245(2016 Sep)
Freed D,Pevsner J

The WASF3-NCKAP1-CYFIP1 Complex Is Essential for Breast Cancer Metastasis.
Cancer research76(17)5133-42(2016 Sep)
Teng Y,Qin H,Bahassan A,Bendzunas NG,Kennedy EJ,Cowell JK

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

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NCKAP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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