ARFGEF1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011517442.2	XP_011515744.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X4
XM_005251134.5	XP_005251191.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
NM_006421.5	NP_006412.2	brefeldin A-inhibited guanine nucleotide-exchange protein 1
NM_006421.4	NP_006412.2	brefeldin A-inhibited guanine nucleotide-exchange protein 1
XM_005251136.4	XP_005251193.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
XM_005251135.3	XP_005251192.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
XM_005251134.4	XP_005251191.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
XM_005251136.3	XP_005251193.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
XM_005251135.4	XP_005251192.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09419	NM_006421.5 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu09419	XM_005251134.5 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu42208	XM_005251135.4 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu42209	XM_005251136.4 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu42210	XM_011517442.2 Latest version!	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu09419	NM_006421.4	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu09419	XM_005251134.4	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu42209	XM_005251136.3	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu42208	XM_005251135.3	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

ARFGEF1 ( NM_006421.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006412.2 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Price & Availability↑

CloneID	OHu09419
Clone ID Related Accession (Same CDS sequence)	XM_005251134.5 , NM_006421.5 , NM_006421.4 , XM_005251134.4
Accession Version	NM_006421.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021321.11, AI692863.1, AF111162.1, AL117446.1 and CA314908.1. On Nov 24, 2018 this sequence version replaced NM_006421.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.152129.1, SRR1803614.267959.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262215.8/ ENSP00000262215.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006412.2
CDS	495..6044
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	NM_006421.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006421.5

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( NM_006421.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006412.2 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

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CloneID	OHu09419
Clone ID Related Accession (Same CDS sequence)	XM_005251134.5 , NM_006421.5 , NM_006421.4 , XM_005251134.4
Accession Version	NM_006421.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI692863.1, AF111162.1, AL117446.1 and CA314908.1. On Aug 23, 2011 this sequence version replaced NM_006421.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.152129.1, SRR1803614.267959.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006412.2
CDS	391..5940
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	NM_006421.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006421.4

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_005251134.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251191.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.

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CloneID	OHu09419
Clone ID Related Accession (Same CDS sequence)	XM_005251134.5 , NM_006421.5 , NM_006421.4 , XM_005251134.4
Accession Version	XM_005251134.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251134.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251191.1
CDS	365..5914
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	XM_005251134.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251134.5

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_005251134.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251191.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.

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CloneID	OHu09419
Clone ID Related Accession (Same CDS sequence)	XM_005251134.5 , NM_006421.5 , NM_006421.4 , XM_005251134.4
Accession Version	XM_005251134.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251134.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251191.1
CDS	370..5919
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	XM_005251134.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251134.4

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_005251135.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251192.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.

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CloneID	OHu42208
Clone ID Related Accession (Same CDS sequence)	XM_005251135.3 , XM_005251135.4
Accession Version	XM_005251135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGA TACAATATCA 
CAGAAGTCTG TAGATATTCA TGATTCTATT CAACCAAGGT CTGTGGATAA CAGACCACAA 
GCACCACTGG TTTCTGCGTC TGCTGTTAAT GAAGAAGTCA GCAAAATTAA ATCTACAGCA 
AAATTTCCAG AACAAAAATT GTTTGCTGCC CTGTTGATTA AATGTGTTGT GCAGCTGGAA 
CTCATCCAGA CTATCGACAA CATTGTCTTC TTCCCAGCCA CAAGTAAGAA AGAAGATGCA 
GAAAACTTAG CTGCAGCACA GAGAGATGCG GTGGACTTTG ATGTTCGCGT TGATACTCAA 
GACCAAGGAA TGTACCGCTT TTTAACATCA CAACAACTTT TTAAGCTACT GGACTGCTTA 
TTAGAGTCAC ATAGATTTGC AAAAGCGTTT AATTCCAACA ACGAACAGAG GACTGCCCTG 
TGGAAAGCAG GATTCAAAGG CAAATCCAAG CCCAACCTTC TGAAGCAGGA GACCAGCAGC 
CTGGCCTGTG GGCTGCGCAT TCTCTTCCGG ATGTACATGG ATGAGAGCCG CGTTAGTGCC 
TGGGAGGAGG TCCAGCAGAG GCTTTTGAAT GTCTGCAGTG AAGCACTAAG TTACTTCCTC 
ACTCTAACAT CAGAAAGTCA TCGAGAAGCC TGGACTAACT TACTGCTTTT GTTTCTAACT 
AAAGTTCTAA AGATAAGTGA TAATAGGTTT AAAGCTCATG CATCATTCTA CTACCCTCTC 
TTATGTGAAA TTATGCAATT TGACTTGATT CCTGAACTTC GTGCTGTTCT TAGAAGATTT 
TTTCTGCGAA TCGGAGTAGT TTTTCAGATA TCACAACCAC CTGAACAGGA ACTTGGAATA 
AACAAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251192.1
CDS	641..6172
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	XM_005251135.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251135.3

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGA TACAATATCA 
CAGAAGTCTG TAGATATTCA TGATTCTATT CAACCAAGGT CTGTGGATAA CAGACCACAA 
GCACCACTGG TTTCTGCGTC TGCTGTTAAT GAAGAAGTCA GCAAAATTAA ATCTACAGCA 
AAATTTCCAG AACAAAAATT GTTTGCTGCC CTGTTGATTA AATGTGTTGT GCAGCTGGAA 
CTCATCCAGA CTATCGACAA CATTGTCTTC TTCCCAGCCA CAAGTAAGAA AGAAGATGCA 
GAAAACTTAG CTGCAGCACA GAGAGATGCG GTGGACTTTG ATGTTCGCGT TGATACTCAA 
GACCAAGGAA TGTACCGCTT TTTAACATCA CAACAACTTT TTAAGCTACT GGACTGCTTA 
TTAGAGTCAC ATAGATTTGC AAAAGCGTTT AATTCCAACA ACGAACAGAG GACTGCCCTG 
TGGAAAGCAG GATTCAAAGG CAAATCCAAG CCCAACCTTC TGAAGCAGGA GACCAGCAGC 
CTGGCCTGTG GGCTGCGCAT TCTCTTCCGG ATGTACATGG ATGAGAGCCG CGTTAGTGCC 
TGGGAGGAGG TCCAGCAGAG GCTTTTGAAT GTCTGCAGTG AAGCACTAAG TTACTTCCTC 
ACTCTAACAT CAGAAAGTCA TCGAGAAGCC TGGACTAACT TACTGCTTTT GTTTCTAACT 
AAAGTTCTAA AGATAAGTGA TAATAGGTTT AAAGCTCATG CATCATTCTA CTACCCTCTC 
TTATGTGAAA TTATGCAATT TGACTTGATT CCTGAACTTC GTGCTGTTCT TAGAAGATTT 
TTTCTGCGAA TCGGAGTAGT TTTTCAGATA TCACAACCAC CTGAACAGGA ACTTGGAATA 
AACAAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_005251135.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251192.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.

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CloneID	OHu42208
Clone ID Related Accession (Same CDS sequence)	XM_005251135.3 , XM_005251135.4
Accession Version	XM_005251135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGA TACAATATCA 
CAGAAGTCTG TAGATATTCA TGATTCTATT CAACCAAGGT CTGTGGATAA CAGACCACAA 
GCACCACTGG TTTCTGCGTC TGCTGTTAAT GAAGAAGTCA GCAAAATTAA ATCTACAGCA 
AAATTTCCAG AACAAAAATT GTTTGCTGCC CTGTTGATTA AATGTGTTGT GCAGCTGGAA 
CTCATCCAGA CTATCGACAA CATTGTCTTC TTCCCAGCCA CAAGTAAGAA AGAAGATGCA 
GAAAACTTAG CTGCAGCACA GAGAGATGCG GTGGACTTTG ATGTTCGCGT TGATACTCAA 
GACCAAGGAA TGTACCGCTT TTTAACATCA CAACAACTTT TTAAGCTACT GGACTGCTTA 
TTAGAGTCAC ATAGATTTGC AAAAGCGTTT AATTCCAACA ACGAACAGAG GACTGCCCTG 
TGGAAAGCAG GATTCAAAGG CAAATCCAAG CCCAACCTTC TGAAGCAGGA GACCAGCAGC 
CTGGCCTGTG GGCTGCGCAT TCTCTTCCGG ATGTACATGG ATGAGAGCCG CGTTAGTGCC 
TGGGAGGAGG TCCAGCAGAG GCTTTTGAAT GTCTGCAGTG AAGCACTAAG TTACTTCCTC 
ACTCTAACAT CAGAAAGTCA TCGAGAAGCC TGGACTAACT TACTGCTTTT GTTTCTAACT 
AAAGTTCTAA AGATAAGTGA TAATAGGTTT AAAGCTCATG CATCATTCTA CTACCCTCTC 
TTATGTGAAA TTATGCAATT TGACTTGATT CCTGAACTTC GTGCTGTTCT TAGAAGATTT 
TTTCTGCGAA TCGGAGTAGT TTTTCAGATA TCACAACCAC CTGAACAGGA ACTTGGAATA 
AACAAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251192.1
CDS	82..5613
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	XM_005251135.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251135.4

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGA TACAATATCA 
CAGAAGTCTG TAGATATTCA TGATTCTATT CAACCAAGGT CTGTGGATAA CAGACCACAA 
GCACCACTGG TTTCTGCGTC TGCTGTTAAT GAAGAAGTCA GCAAAATTAA ATCTACAGCA 
AAATTTCCAG AACAAAAATT GTTTGCTGCC CTGTTGATTA AATGTGTTGT GCAGCTGGAA 
CTCATCCAGA CTATCGACAA CATTGTCTTC TTCCCAGCCA CAAGTAAGAA AGAAGATGCA 
GAAAACTTAG CTGCAGCACA GAGAGATGCG GTGGACTTTG ATGTTCGCGT TGATACTCAA 
GACCAAGGAA TGTACCGCTT TTTAACATCA CAACAACTTT TTAAGCTACT GGACTGCTTA 
TTAGAGTCAC ATAGATTTGC AAAAGCGTTT AATTCCAACA ACGAACAGAG GACTGCCCTG 
TGGAAAGCAG GATTCAAAGG CAAATCCAAG CCCAACCTTC TGAAGCAGGA GACCAGCAGC 
CTGGCCTGTG GGCTGCGCAT TCTCTTCCGG ATGTACATGG ATGAGAGCCG CGTTAGTGCC 
TGGGAGGAGG TCCAGCAGAG GCTTTTGAAT GTCTGCAGTG AAGCACTAAG TTACTTCCTC 
ACTCTAACAT CAGAAAGTCA TCGAGAAGCC TGGACTAACT TACTGCTTTT GTTTCTAACT 
AAAGTTCTAA AGATAAGTGA TAATAGGTTT AAAGCTCATG CATCATTCTA CTACCCTCTC 
TTATGTGAAA TTATGCAATT TGACTTGATT CCTGAACTTC GTGCTGTTCT TAGAAGATTT 
TTTCTGCGAA TCGGAGTAGT TTTTCAGATA TCACAACCAC CTGAACAGGA ACTTGGAATA 
AACAAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_005251136.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251193.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X3, mRNA.

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CloneID	OHu42209
Clone ID Related Accession (Same CDS sequence)	XM_005251136.4 , XM_005251136.3
Accession Version	XM_005251136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251136.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGACCAA ACGGACCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251193.1
CDS	82..5484
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRTKRTL

Target ORF information:

RefSeq Version	XM_005251136.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251136.4

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGACCAA ACGGACCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_005251136.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251193.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X3, mRNA.

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CloneID	OHu42209
Clone ID Related Accession (Same CDS sequence)	XM_005251136.4 , XM_005251136.3
Accession Version	XM_005251136.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGACCAA ACGGACCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251193.1
CDS	641..6043
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRTKRTL

Target ORF information:

RefSeq Version	XM_005251136.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251136.3

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCTCTGGA GAAGATATTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAA CAGAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTGCCTT TTGAGTTGGC ATGCCAGTCC AAATGTCCTC GCATAGTTAG TACATCTCTA 
GATTGCTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTTCAGGGC 
CCTCAGACAG ATGAAGGAGT TCAGCTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA TGAAGGGACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATCTACTTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTCACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGACCAA ACGGACCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_011517442.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515744.1 Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X4, mRNA.

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CloneID	OHu42210
Clone ID Related Accession (Same CDS sequence)	XM_011517442.2
Accession Version	XM_011517442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515744.1
CDS	1277..6226
Translation	MLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	XM_011517442.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517442.2

ORF Insert Sequence:

ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC AAAACAAATG 
GAAAAAGAAA GGCATCGGCA GCATCATCAT CTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCTGAATCAC CTCAACTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT ATCCCAAGAA 
CACGAAGGGG ACCTTGACCT CCATACAAAT GATGTAGATA AAAGTCTTCA GGATGACACA 
GAGCCTGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GAAGTGTTAT ATGACGGAGA AAACCATGAT 
TGTGAGGAAA AGCCACAAGA CATTGTACAG AACATTGTAG AAGAAATGGT GAACATTGTT 
GTTGGAGATA TGGGAGAAGG GACTACTATA AATGCAAGTG CAGATGGCAA CATTGGAACT 
ATAGAGGATG GTAGTGACAG TGAAAATATT CAAGCAAATG GAATTCCAGG AACACCAATT 
TCTGTTGCAT ATACACCATC CTTACCTGAT GATAGATTGT CAGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTGTCAGAT 
GGACCACCAG ATCCAAAGTC TCATGAACTA CGATCCAAGA TTCTTTCATT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATTAAGCAGT ATCTTTGTGT TGCACTCTCA AAAAATGGAG TCTCATCTGT TCCAGAGGTT 
TTTGAGCTTT CTCTTTCTAT ATTTCTTACT TTGTTGTCAA ATTTCAAGAC ACATCTGAAG 
ATGCAAATTG AGGTGTTCTT TAAAGAAATT TTCTTATACA TTTTGGAAAC TTCTACCAGC 
TCATTTGATC ACAAATGGAT GGTTATTCAG ACACTGACGA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATTTATGT AAACTATGAC TGTGACTTAA ATGCAGCCAA TATATTTGAA 
AGACTAGTAA ATGATCTATC AAAAATTGCT CAAGGAAGGG GCAGTCAAGA ACTTGGTATG 
AGTAATGTTC AGGAATTGAG CCTGAGGAAA AAAGGTTTAG AATGCTTAGT GTCGATTTTG 
AAGTGTATGG TTGAATGGAG TAAGGATCAG TATGTGAATC CCAACTCCCA GACAACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGATG AGTGAAATCA AACACCCTGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACATCATCAT CAGGAATAGG CAGCTACAGT 
ACACAGATGT CTGGCACTGA TAATCCAGAA CAATTTGAGG TCCTAAAGCA ACAAAAAGAA 
ATAATAGAAC AAGGGATAGA TTTATTTAAT AAGAAACCAA AGAGAGGAAT ACAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACACCT GAAGATATTG CCCAATTCTT ACATCAAGAG 
GAAAGATTAG ACTCTACTCA AGTGGGTGAG TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCATATGT GGACCAACAT GACTTTTCAG GAAAAGACTT CGTTTCAGCC 
CTTCGTATGT TTCTAGAAGG ATTTCGTCTT CCAGGGGAAG CTCAGAAAAT CGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATACCTAGAA TGCAACCAAG GACAAACTCT CTTTGCTAGT 
GCGGATACAG CTTATGTTTT GGCTTATTCA ATTATCATGT TGACCACAGA CCTTCACAGT 
CCACAGGTGA AAAATAAAAT GACAAAGGAA CAATACATTA AGATGAATAG AGGTATCAAT 
GACAGTAAAG ACCTTCCTGA AGAGTATCTA TCAGCCATCT ATAATGAAAT AGCTGGGAAA 
AAGATATCAA TGAAAGAAAC AAAAGAACTA ACAATCCCTA CAAAATCAAG TAAACAGAAT 
GTAGCCAGTG AAAAACAAAG AAGACTTCTG TATAACTTAG AAATGGAGCA GATGGCCAAG 
ACAGCTAAAG CACTCATGGA GGCCGTGAGC CATGTTCAGG CACCCTTTAC AAGTGCAACA 
CATTTGGAGC ATGTGAGGCC CATGTTTAAG TTGGCTTGGA CGCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGATTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAGAGAGA TGCATATGTC 
CAGGCACTAG CAAGATTTAC CTTACTCACA GTGAGTTCCG GTATTACTGA AATGAAACAG 
AAGAACATTG ACACAATAAA AACACTTATC ACAGTGGCTC ATACAGATGG AAATTATTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGT TGGAGCTGGC ACAGCTTATA 
GGAACTGGAG TGAAACCTCG ATACATTTCT GGAACAGTGC GAGGCAGAGA AGGATCTCTT 
ACTGGAACAA AAGATCAGGC TCCTGATGAA TTTGTGGGTT TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AACAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAGAGT 
GTTGTTGTTG CTGTAGATAG AATATTCACA GGATCTACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC CACGACACAC 
CCAAGAATGT TTAGTCTACA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTACAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGGTGT 
AATCCTAATG AAGATGTAGC TATTTTTGCA GTAGACTCCT TGAGGCAGTT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAACTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGAG ATATGGTTGT ACGGTGTATA 
GCACAGATGG TTAATTCTCA AGCTGCTAAC ATTCGATCTG GATGGAAGAA CATTTTCTCT 
GTATTTCATC TAGCTGCATC TGATCAAGAT GAAAGCATAG TGGAACTTGC ATTCCAAACA 
ACCGGGCACA TTGTCACCCT TGTATTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAGTGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGACACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATATG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAAT ACACAAGCGA TGATATGAAC GTAGCACCTG AAGACAGGGT GTGGGTGAGA 
GGATGGTTCC CAATTCTCTT TGAGTTATCC TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CATATGGCCA CACTTATGAG 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTG 
CCAGAACAAC AGACAGAGAA AGCTGAATGG ATGACAACAA CTTGCAATCA TGCACTTTAT 
GCAATCTGTG ATGTATTCAC TCAGTATTTA GAAGTACTCA GTGATGTACT TTTGGATGAC 
ATTTTTGCTC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTAGC GCGATCTGGT 
ACAAACTGTT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTTACCCT AGAAATCTGG 
GATAAAACTT GCAACTGCAC ACTGGATATC TTCAAAACCA CAATCCCACA TGCGCTGTTG 
ACCTGGCGAC CCAATTCTGG AGAAACTGCC CCCCCACCTC CATCTCCTGT AAGTGAAAAG 
CCATTGGATA CAATATCACA GAAGTCTGTA GATATTCATG ATTCTATTCA ACCAAGGTCT 
GTGGATAACA GACCACAAGC ACCACTGGTT TCTGCGTCTG CTGTTAATGA AGAAGTCAGC 
AAAATTAAAT CTACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCTGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TTGTCTTCTT CCCAGCCACA 
AGTAAGAAAG AAGATGCAGA AAACTTAGCT GCAGCACAGA GAGATGCGGT GGACTTTGAT 
GTTCGCGTTG ATACTCAAGA CCAAGGAATG TACCGCTTTT TAACATCACA ACAACTTTTT 
AAGCTACTGG ACTGCTTATT AGAGTCACAT AGATTTGCAA AAGCGTTTAA TTCCAACAAC 
GAACAGAGGA CTGCCCTGTG GAAAGCAGGA TTCAAAGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CCAGCAGCCT GGCCTGTGGG CTGCGCATTC TCTTCCGGAT GTACATGGAT 
GAGAGCCGCG TTAGTGCCTG GGAGGAGGTC CAGCAGAGGC TTTTGAATGT CTGCAGTGAA 
GCACTAAGTT ACTTCCTCAC TCTAACATCA GAAAGTCATC GAGAAGCCTG GACTAACTTA 
CTGCTTTTGT TTCTAACTAA AGTTCTAAAG ATAAGTGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGCGAATC GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ARFGEF1
Entrez Gene ID	10565
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms	ARFGEP1,BIG1,P200
General protein information	Preferred Names ADP ribosylation factor guanine nucleotide exchange factor 1 Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) p200 ARF guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q13.2
Summary	ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Aug 2011].
Disorder MIM:	604141

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ARFGEF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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