SEMA6B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA6B
Entrez Gene ID	10501
Full Name	semaphorin 6B
Synonyms	SEM-SEMA-Y,SEMA-VIB,SEMAN,semaZ
General protein information	Preferred Names semaphorin 6B Names semaphorin-6B sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B semaphorin VIB semaphorin Z semaphorin-6Ba
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a member of the semaphorin family, a group of proteins characterized by the presence of a conserved semaphorin (sema) domain. Whereas some semaphorins are transmembrane proteins, others are secreted. Semaphorins play a major role in axon guidance. The protein encoded by this gene may be involved in both peripheral and central nervous system development. [provided by RefSeq, Jul 2008].
Disorder MIM:	608873

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527641.1	XP_011525943.1	semaphorin-6B isoform X2
NM_032108.4	NP_115484.2	semaphorin-6B precursor
XM_011527639.2	XP_011525941.1	semaphorin-6B isoform X1
XM_011527640.2	XP_011525942.1	semaphorin-6B isoform X1
NM_032108.3	NP_115484.2	semaphorin-6B precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SEMA6B	XP_002688976.2
Mus musculus (house mouse)	Sema6b	NP_038690.1
Gallus gallus (chicken)	SEMA6B	XP_004948862.1
Xenopus tropicalis (tropical clawed frog)	sema6b	XP_002942249.2
Danio rerio (zebrafish)	si:ch211-168n16.1	XP_002660976.2
	LOC719044	XP_001112607.1
Rattus norvegicus (Norway rat)	Sema6b	NP_445923.1
Homo sapiens (human)	SEMA6B	NP_115484.2
Pan troglodytes (chimpanzee)	SEMA6B	XP_001139016.3
Danio rerio (zebrafish)	LOC100148883	XP_002663156.2

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of SEMA6B gene expression in breast cancer: identification of a new isoform.
D'Apice L, Costa V, Valente C, Trovato M, Pagani A, Manera S, Regolo L, Zambelli A, Ciccodicola A, De Berardinis P
Biochimica et biophysica acta1830(10)4543-53(2013 Oct)

The role of axon guidance factor semaphorin 6B in the invasion and metastasis of gastric cancer.
Ge C, Li Q, Wang L, Xu X
The Journal of international medical research41(2)284-92(2013 Apr)

Signal peptide prediction based on analysis of experimentally verified cleavage sites.
Zhang Z, Henzel WJ
Protein science : a publication of the Protein Society13(10)2819-24(2004 Oct)

The human semaphorin 6B gene is down regulated by PPARs.
Collet P, Domenjoud L, Devignes MD, Murad H, Schohn H, Dau?a M
Genomics83(6)1141-50(2004 Jun)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

states a clear relation among breast cancer and SEMA6B expression; moreover we describe for the first time the SEMA6Ba protein and report here the analysis of SEMA6Ba RNA messenger, the protein expression and the cellular localization
Title: Analysis of SEMA6B gene expression in breast cancer: identification of a new isoform.

Semaphorin 6B is related to tumour differentiation and metastasis in vivo, and tumour cell migration, adhesion and invasion in vitro.
Title: The role of axon guidance factor semaphorin 6B in the invasion and metastasis of gastric cancer.

Gene SEMA6B is strongly down regulated by peroxisome proliferator-activated receptors.
Title: The human semaphorin 6B gene is down regulated by PPARs.

The following SEMA6B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA6B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04848	NM_032108.4 Latest version!	Homo sapiens semaphorin 6B (SEMA6B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu64072	XM_011527641.1 Latest version!	Homo sapiens semaphorin 6B (SEMA6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64071	XM_011527640.2 Latest version!	Homo sapiens semaphorin 6B (SEMA6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu64071	XM_011527639.2 Latest version!	Homo sapiens semaphorin 6B (SEMA6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu04848	NM_032108.3	Homo sapiens semaphorin 6B (SEMA6B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA6B ( NM_032108.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115484.2 Homo sapiens semaphorin 6B (SEMA6B), mRNA.

Price & Availability↑

CloneID	OHu04848
Clone ID Related Accession (Same CDS sequence)	NM_032108.4 , NM_032108.3
Accession Version	NM_032108.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	semaphorin-6B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA818632.1, DB257858.1 and BC142617.1. On Nov 22, 2018 this sequence version replaced NM_032108.3. ##Evidence-Data-START## Transcript exon combination :: BC142617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000586582.6/ ENSP00000467290.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGAACTGTAT CGGCAGTCAG 
GACCCCTACT GCGGGTGGGC CCCCGACGGC TCCTGCATCT TCCTCAGCCC GGGCACCAGA 
GCCGCCTTTG AGCAGGACGT GTCCGGGGCC AGCACCTCAG GCTTAGGGGA CTGCACAGGA 
CTCCTGCGGG CCAGCCTCTC CGAGGACCGC GCGGGGCTGG TGTCGGTGAA CCTGCTGGTA 
ACGTCGTCGG TGGCGGCCTT CGTGGTGGGA GCCGTGGTGT CCGGCTTCAG CGTGGGCTGG 
TTCGTGGGCC TCCGTGAGCG GCGGGAGCTG GCCCGGCGCA AGGACAAGGA GGCCATCCTG 
GCGCACGGGG CGGGCGAGGC GGTGCTGAGC GTCAGCCGCC TGGGCGAGCG CAGGGCGCAG 
GGTCCCGGGG GCCGGGGCGG AGGCGGTGGC GGTGGCGCCG GGGTTCCCCC GGAGGCCCTG 
CTGGCGCCCC TGATGCAGAA CGGCTGGGCC AAGGCCACGC TGCTGCAGGG CGGGCCCCAC 
GACCTGGACT CGGGGCTGCT GCCCACGCCC GAGCAGACGC CGCTGCCGCA GAAGCGCCTG 
CCCACTCCGC ACCCGCACCC CCACGCCCTG GGCCCCCGCG CCTGGGACCA CGGCCACCCC 
CTGCTCCCGG CCTCCGCTTC ATCCTCCCTC CTGCTGCTGG CGCCCGCCCG GGCCCCCGAG 
CAGCCCCCCG CGCCTGGGGA GCCGACCCCC GACGGCCGCC TCTATGCTGC CCGGCCCGGC 
CGCGCCTCCC ACGGCGACTT CCCGCTCACC CCCCACGCCA GCCCGGACCG CCGGCGGGTG 
GTGTCCGCGC CCACGGGCCC CTTGGACCCA GCCTCAGCCG CCGATGGCCT CCCGCGGCCC 
TGGAGCCCGC CCCCGACGGG CAGCCTGAGG AGGCCACTGG GCCCCCACGC CCCTCCGGCC 
GCCACCCTGC GCCGCACCCA CACGTTCAAC AGCGGCGAGG CCCGGCCTGG GGACCGCCAC 
CGCGGCTGCC ACGCCCGGCC GGGCACAGAC TTGGCCCACC TCCTCCCCTA TGGGGGGGCG 
GACAGGACTG CGCCCCCCGT GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115484.2
CDS	188..2854
Translation	MQTPRASPPRPALLLLLLLLGGAHGLFPEEPPPLSVAPRDYLNHYPVFVGSGPGRLTPAEGADDLNIQRVLRVNRTLFIGDRDNLYRVELEPPTSTELRYQRKLTWRSNPSDINVCRMKGKQEGECRNFVKVLLLRDESTLFVCGSNAFNPVCANYSIDTLQPVGDNISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRSLGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLEKQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVVLAVFSTPSNSIPGSAVCAFDLTQVAAVFEGRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASSALPDDILNFVKTHPLMDEAVPSLGHAPWILRTLMRHQLTRVAVDVGAGPWGNQTVVFLGSEAGTVLKFLVRPNASTSGTSGLSVFLEEFETYRPDRCGRPGGGETGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQQYSGCMKNCIGSQDPYCGWAPDGSCIFLSPGTRAAFEQDVSGASTSGLGDCTGLLRASLSEDRAGLVSVNLLVTSSVAAFVVGAVVSGFSVGWFVGLRERRELARRKDKEAILAHGAGEAVLSVSRLGERRAQGPGGRGGGGGGGAGVPPEALLAPLMQNGWAKATLLQGGPHDLDSGLLPTPEQTPLPQKRLPTPHPHPHALGPRAWDHGHPLLPASASSSLLLLAPARAPEQPPAPGEPTPDGRLYAARPGRASHGDFPLTPHASPDRRRVVSAPTGPLDPASAADGLPRPWSPPPTGSLRRPLGPHAPPAATLRRTHTFNSGEARPGDRHRGCHARPGTDLAHLLPYGGADRTAPPVP

Target ORF information:

RefSeq Version	NM_032108.4
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 6B (SEMA6B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032108.4

ORF Insert Sequence:

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGAACTGTAT CGGCAGTCAG 
GACCCCTACT GCGGGTGGGC CCCCGACGGC TCCTGCATCT TCCTCAGCCC GGGCACCAGA 
GCCGCCTTTG AGCAGGACGT GTCCGGGGCC AGCACCTCAG GCTTAGGGGA CTGCACAGGA 
CTCCTGCGGG CCAGCCTCTC CGAGGACCGC GCGGGGCTGG TGTCGGTGAA CCTGCTGGTA 
ACGTCGTCGG TGGCGGCCTT CGTGGTGGGA GCCGTGGTGT CCGGCTTCAG CGTGGGCTGG 
TTCGTGGGCC TCCGTGAGCG GCGGGAGCTG GCCCGGCGCA AGGACAAGGA GGCCATCCTG 
GCGCACGGGG CGGGCGAGGC GGTGCTGAGC GTCAGCCGCC TGGGCGAGCG CAGGGCGCAG 
GGTCCCGGGG GCCGGGGCGG AGGCGGTGGC GGTGGCGCCG GGGTTCCCCC GGAGGCCCTG 
CTGGCGCCCC TGATGCAGAA CGGCTGGGCC AAGGCCACGC TGCTGCAGGG CGGGCCCCAC 
GACCTGGACT CGGGGCTGCT GCCCACGCCC GAGCAGACGC CGCTGCCGCA GAAGCGCCTG 
CCCACTCCGC ACCCGCACCC CCACGCCCTG GGCCCCCGCG CCTGGGACCA CGGCCACCCC 
CTGCTCCCGG CCTCCGCTTC ATCCTCCCTC CTGCTGCTGG CGCCCGCCCG GGCCCCCGAG 
CAGCCCCCCG CGCCTGGGGA GCCGACCCCC GACGGCCGCC TCTATGCTGC CCGGCCCGGC 
CGCGCCTCCC ACGGCGACTT CCCGCTCACC CCCCACGCCA GCCCGGACCG CCGGCGGGTG 
GTGTCCGCGC CCACGGGCCC CTTGGACCCA GCCTCAGCCG CCGATGGCCT CCCGCGGCCC 
TGGAGCCCGC CCCCGACGGG CAGCCTGAGG AGGCCACTGG GCCCCCACGC CCCTCCGGCC 
GCCACCCTGC GCCGCACCCA CACGTTCAAC AGCGGCGAGG CCCGGCCTGG GGACCGCCAC 
CGCGGCTGCC ACGCCCGGCC GGGCACAGAC TTGGCCCACC TCCTCCCCTA TGGGGGGGCG 
GACAGGACTG CGCCCCCCGT GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6B ( NM_032108.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115484.2 Homo sapiens semaphorin 6B (SEMA6B), mRNA.

Price & Availability↑

CloneID	OHu04848
Clone ID Related Accession (Same CDS sequence)	NM_032108.4 , NM_032108.3
Accession Version	NM_032108.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	semaphorin-6B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA818632.1, DB257858.1, BC142617.1 and AI825930.1. On Nov 25, 2010 this sequence version replaced NM_032108.2. ##Evidence-Data-START## Transcript exon combination :: BC142617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGAACTGTAT CGGCAGTCAG 
GACCCCTACT GCGGGTGGGC CCCCGACGGC TCCTGCATCT TCCTCAGCCC GGGCACCAGA 
GCCGCCTTTG AGCAGGACGT GTCCGGGGCC AGCACCTCAG GCTTAGGGGA CTGCACAGGA 
CTCCTGCGGG CCAGCCTCTC CGAGGACCGC GCGGGGCTGG TGTCGGTGAA CCTGCTGGTA 
ACGTCGTCGG TGGCGGCCTT CGTGGTGGGA GCCGTGGTGT CCGGCTTCAG CGTGGGCTGG 
TTCGTGGGCC TCCGTGAGCG GCGGGAGCTG GCCCGGCGCA AGGACAAGGA GGCCATCCTG 
GCGCACGGGG CGGGCGAGGC GGTGCTGAGC GTCAGCCGCC TGGGCGAGCG CAGGGCGCAG 
GGTCCCGGGG GCCGGGGCGG AGGCGGTGGC GGTGGCGCCG GGGTTCCCCC GGAGGCCCTG 
CTGGCGCCCC TGATGCAGAA CGGCTGGGCC AAGGCCACGC TGCTGCAGGG CGGGCCCCAC 
GACCTGGACT CGGGGCTGCT GCCCACGCCC GAGCAGACGC CGCTGCCGCA GAAGCGCCTG 
CCCACTCCGC ACCCGCACCC CCACGCCCTG GGCCCCCGCG CCTGGGACCA CGGCCACCCC 
CTGCTCCCGG CCTCCGCTTC ATCCTCCCTC CTGCTGCTGG CGCCCGCCCG GGCCCCCGAG 
CAGCCCCCCG CGCCTGGGGA GCCGACCCCC GACGGCCGCC TCTATGCTGC CCGGCCCGGC 
CGCGCCTCCC ACGGCGACTT CCCGCTCACC CCCCACGCCA GCCCGGACCG CCGGCGGGTG 
GTGTCCGCGC CCACGGGCCC CTTGGACCCA GCCTCAGCCG CCGATGGCCT CCCGCGGCCC 
TGGAGCCCGC CCCCGACGGG CAGCCTGAGG AGGCCACTGG GCCCCCACGC CCCTCCGGCC 
GCCACCCTGC GCCGCACCCA CACGTTCAAC AGCGGCGAGG CCCGGCCTGG GGACCGCCAC 
CGCGGCTGCC ACGCCCGGCC GGGCACAGAC TTGGCCCACC TCCTCCCCTA TGGGGGGGCG 
GACAGGACTG CGCCCCCCGT GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115484.2
CDS	263..2929
Translation	MQTPRASPPRPALLLLLLLLGGAHGLFPEEPPPLSVAPRDYLNHYPVFVGSGPGRLTPAEGADDLNIQRVLRVNRTLFIGDRDNLYRVELEPPTSTELRYQRKLTWRSNPSDINVCRMKGKQEGECRNFVKVLLLRDESTLFVCGSNAFNPVCANYSIDTLQPVGDNISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRSLGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLEKQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVVLAVFSTPSNSIPGSAVCAFDLTQVAAVFEGRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASSALPDDILNFVKTHPLMDEAVPSLGHAPWILRTLMRHQLTRVAVDVGAGPWGNQTVVFLGSEAGTVLKFLVRPNASTSGTSGLSVFLEEFETYRPDRCGRPGGGETGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQQYSGCMKNCIGSQDPYCGWAPDGSCIFLSPGTRAAFEQDVSGASTSGLGDCTGLLRASLSEDRAGLVSVNLLVTSSVAAFVVGAVVSGFSVGWFVGLRERRELARRKDKEAILAHGAGEAVLSVSRLGERRAQGPGGRGGGGGGGAGVPPEALLAPLMQNGWAKATLLQGGPHDLDSGLLPTPEQTPLPQKRLPTPHPHPHALGPRAWDHGHPLLPASASSSLLLLAPARAPEQPPAPGEPTPDGRLYAARPGRASHGDFPLTPHASPDRRRVVSAPTGPLDPASAADGLPRPWSPPPTGSLRRPLGPHAPPAATLRRTHTFNSGEARPGDRHRGCHARPGTDLAHLLPYGGADRTAPPVP

Target ORF information:

RefSeq Version	NM_032108.3
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 6B (SEMA6B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032108.3

ORF Insert Sequence:

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGAACTGTAT CGGCAGTCAG 
GACCCCTACT GCGGGTGGGC CCCCGACGGC TCCTGCATCT TCCTCAGCCC GGGCACCAGA 
GCCGCCTTTG AGCAGGACGT GTCCGGGGCC AGCACCTCAG GCTTAGGGGA CTGCACAGGA 
CTCCTGCGGG CCAGCCTCTC CGAGGACCGC GCGGGGCTGG TGTCGGTGAA CCTGCTGGTA 
ACGTCGTCGG TGGCGGCCTT CGTGGTGGGA GCCGTGGTGT CCGGCTTCAG CGTGGGCTGG 
TTCGTGGGCC TCCGTGAGCG GCGGGAGCTG GCCCGGCGCA AGGACAAGGA GGCCATCCTG 
GCGCACGGGG CGGGCGAGGC GGTGCTGAGC GTCAGCCGCC TGGGCGAGCG CAGGGCGCAG 
GGTCCCGGGG GCCGGGGCGG AGGCGGTGGC GGTGGCGCCG GGGTTCCCCC GGAGGCCCTG 
CTGGCGCCCC TGATGCAGAA CGGCTGGGCC AAGGCCACGC TGCTGCAGGG CGGGCCCCAC 
GACCTGGACT CGGGGCTGCT GCCCACGCCC GAGCAGACGC CGCTGCCGCA GAAGCGCCTG 
CCCACTCCGC ACCCGCACCC CCACGCCCTG GGCCCCCGCG CCTGGGACCA CGGCCACCCC 
CTGCTCCCGG CCTCCGCTTC ATCCTCCCTC CTGCTGCTGG CGCCCGCCCG GGCCCCCGAG 
CAGCCCCCCG CGCCTGGGGA GCCGACCCCC GACGGCCGCC TCTATGCTGC CCGGCCCGGC 
CGCGCCTCCC ACGGCGACTT CCCGCTCACC CCCCACGCCA GCCCGGACCG CCGGCGGGTG 
GTGTCCGCGC CCACGGGCCC CTTGGACCCA GCCTCAGCCG CCGATGGCCT CCCGCGGCCC 
TGGAGCCCGC CCCCGACGGG CAGCCTGAGG AGGCCACTGG GCCCCCACGC CCCTCCGGCC 
GCCACCCTGC GCCGCACCCA CACGTTCAAC AGCGGCGAGG CCCGGCCTGG GGACCGCCAC 
CGCGGCTGCC ACGCCCGGCC GGGCACAGAC TTGGCCCACC TCCTCCCCTA TGGGGGGGCG 
GACAGGACTG CGCCCCCCGT GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6B ( XM_011527641.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525943.1 Homo sapiens semaphorin 6B (SEMA6B), transcript variant X3, mRNA.

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CloneID	OHu64072
Clone ID Related Accession (Same CDS sequence)	XM_011527641.1
Accession Version	XM_011527641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-6B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGTCTGCTCT AGGAACTGTA 
TCGGCAGTCA GGACCCCTAC TGCGGGTGGG CCCCCGACGG CTCCTGCATC TTCCTCAGCC 
CGGGCACCAG AGCCGCCTTT GAGCAGGACG TGTCCGGGGC CAGCACCTCA GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525943.1
CDS	312..2048
Translation	MQTPRASPPRPALLLLLLLLGGAHGLFPEEPPPLSVAPRDYLNHYPVFVGSGPGRLTPAEGADDLNIQRVLRVNRTLFIGDRDNLYRVELEPPTSTELRYQRKLTWRSNPSDINVCRMKGKQEGECRNFVKVLLLRDESTLFVCGSNAFNPVCANYSIDTLQPVGDNISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRSLGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLEKQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVVLAVFSTPSNSIPGSAVCAFDLTQVAAVFEGRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASSALPDDILNFVKTHPLMDEAVPSLGHAPWILRTLMRHQLTRVAVDVGAGPWGNQTVVFLGSEAGTVLKFLVRPNASTSGTSGLSVFLEEFETYRPDRCGRPGGGETGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQQYSGCMKSALGTVSAVRTPTAGGPPTAPASSSARAPEPPLSRTCPGPAPQA

Target ORF information:

RefSeq Version	XM_011527641.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 6B (SEMA6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527641.1

ORF Insert Sequence:

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGTCTGCTCT AGGAACTGTA 
TCGGCAGTCA GGACCCCTAC TGCGGGTGGG CCCCCGACGG CTCCTGCATC TTCCTCAGCC 
CGGGCACCAG AGCCGCCTTT GAGCAGGACG TGTCCGGGGC CAGCACCTCA GGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6B ( XM_011527639.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525941.1 Homo sapiens semaphorin 6B (SEMA6B), transcript variant X1, mRNA.

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CloneID	OHu64071
Clone ID Related Accession (Same CDS sequence)	XM_011527639.2 , XM_011527640.2
Accession Version	XM_011527639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGTCCCTGGT CTGCTCTAGG 
AACTGTATCG GCAGTCAGGA CCCCTACTGC GGGTGGGCCC CCGACGGCTC CTGCATCTTC 
CTCAGCCCGG GCACCAGAGC CGCCTTTGAG CAGGACGTGT CCGGGGCCAG CACCTCAGGC 
TTAGGGGACT GCACAGGACT CCTGCGGGCC AGCCTCTCCG AGGACCGCGC GGGGCTGGTG 
TCGGTGAACC TGCTGGTAAC GTCGTCGGTG GCGGCCTTCG TGGTGGGAGC CGTGGTGTCC 
GGCTTCAGCG TGGGCTGGTT CGTGGGCCTC CGTGAGCGGC GGGAGCTGGC CCGGCGCAAG 
GACAAGGAGG CCATCCTGGC GCACGGGGCG GGCGAGGCGG TGCTGAGCGT CAGCCGCCTG 
GGCGAGCGCA GGGCGCAGGG TCCCGGGGGC CGGGGCGGAG GCGGTGGCGG TGGCGCCGGG 
GTTCCCCCGG AGGCCCTGCT GGCGCCCCTG ATGCAGAACG GCTGGGCCAA GGCCACGCTG 
CTGCAGGGCG GGCCCCACGA CCTGGACTCG GGGCTGCTGC CCACGCCCGA GCAGACGCCG 
CTGCCGCAGA AGCGCCTGCC CACTCCGCAC CCGCACCCCC ACGCCCTGGG CCCCCGCGCC 
TGGGACCACG GCCACCCCCT GCTCCCGGCC TCCGCTTCAT CCTCCCTCCT GCTGCTGGCG 
CCCGCCCGGG CCCCCGAGCA GCCCCCCGCG CCTGGGGAGC CGACCCCCGA CGGCCGCCTC 
TATGCTGCCC GGCCCGGCCG CGCCTCCCAC GGCGACTTCC CGCTCACCCC CCACGCCAGC 
CCGGACCGCC GGCGGGTGGT GTCCGCGCCC ACGGGCCCCT TGGACCCAGC CTCAGCCGCC 
GATGGCCTCC CGCGGCCCTG GAGCCCGCCC CCGACGGGCA GCCTGAGGAG GCCACTGGGC 
CCCCACGCCC CTCCGGCCGC CACCCTGCGC CGCACCCACA CGTTCAACAG CGGCGAGGCC 
CGGCCTGGGG ACCGCCACCG CGGCTGCCAC GCCCGGCCGG GCACAGACTT GGCCCACCTC 
CTCCCCTATG GGGGGGCGGA CAGGACTGCG CCCCCCGTGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525941.1
CDS	312..2996
Translation	MQTPRASPPRPALLLLLLLLGGAHGLFPEEPPPLSVAPRDYLNHYPVFVGSGPGRLTPAEGADDLNIQRVLRVNRTLFIGDRDNLYRVELEPPTSTELRYQRKLTWRSNPSDINVCRMKGKQEGECRNFVKVLLLRDESTLFVCGSNAFNPVCANYSIDTLQPVGDNISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRSLGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLEKQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVVLAVFSTPSNSIPGSAVCAFDLTQVAAVFEGRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASSALPDDILNFVKTHPLMDEAVPSLGHAPWILRTLMRHQLTRVAVDVGAGPWGNQTVVFLGSEAGTVLKFLVRPNASTSGTSGLSVFLEEFETYRPDRCGRPGGGETGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQQYSGCMKSLVCSRNCIGSQDPYCGWAPDGSCIFLSPGTRAAFEQDVSGASTSGLGDCTGLLRASLSEDRAGLVSVNLLVTSSVAAFVVGAVVSGFSVGWFVGLRERRELARRKDKEAILAHGAGEAVLSVSRLGERRAQGPGGRGGGGGGGAGVPPEALLAPLMQNGWAKATLLQGGPHDLDSGLLPTPEQTPLPQKRLPTPHPHPHALGPRAWDHGHPLLPASASSSLLLLAPARAPEQPPAPGEPTPDGRLYAARPGRASHGDFPLTPHASPDRRRVVSAPTGPLDPASAADGLPRPWSPPPTGSLRRPLGPHAPPAATLRRTHTFNSGEARPGDRHRGCHARPGTDLAHLLPYGGADRTAPPVP

Target ORF information:

RefSeq Version	XM_011527639.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 6B (SEMA6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527639.2

ORF Insert Sequence:

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGTCCCTGGT CTGCTCTAGG 
AACTGTATCG GCAGTCAGGA CCCCTACTGC GGGTGGGCCC CCGACGGCTC CTGCATCTTC 
CTCAGCCCGG GCACCAGAGC CGCCTTTGAG CAGGACGTGT CCGGGGCCAG CACCTCAGGC 
TTAGGGGACT GCACAGGACT CCTGCGGGCC AGCCTCTCCG AGGACCGCGC GGGGCTGGTG 
TCGGTGAACC TGCTGGTAAC GTCGTCGGTG GCGGCCTTCG TGGTGGGAGC CGTGGTGTCC 
GGCTTCAGCG TGGGCTGGTT CGTGGGCCTC CGTGAGCGGC GGGAGCTGGC CCGGCGCAAG 
GACAAGGAGG CCATCCTGGC GCACGGGGCG GGCGAGGCGG TGCTGAGCGT CAGCCGCCTG 
GGCGAGCGCA GGGCGCAGGG TCCCGGGGGC CGGGGCGGAG GCGGTGGCGG TGGCGCCGGG 
GTTCCCCCGG AGGCCCTGCT GGCGCCCCTG ATGCAGAACG GCTGGGCCAA GGCCACGCTG 
CTGCAGGGCG GGCCCCACGA CCTGGACTCG GGGCTGCTGC CCACGCCCGA GCAGACGCCG 
CTGCCGCAGA AGCGCCTGCC CACTCCGCAC CCGCACCCCC ACGCCCTGGG CCCCCGCGCC 
TGGGACCACG GCCACCCCCT GCTCCCGGCC TCCGCTTCAT CCTCCCTCCT GCTGCTGGCG 
CCCGCCCGGG CCCCCGAGCA GCCCCCCGCG CCTGGGGAGC CGACCCCCGA CGGCCGCCTC 
TATGCTGCCC GGCCCGGCCG CGCCTCCCAC GGCGACTTCC CGCTCACCCC CCACGCCAGC 
CCGGACCGCC GGCGGGTGGT GTCCGCGCCC ACGGGCCCCT TGGACCCAGC CTCAGCCGCC 
GATGGCCTCC CGCGGCCCTG GAGCCCGCCC CCGACGGGCA GCCTGAGGAG GCCACTGGGC 
CCCCACGCCC CTCCGGCCGC CACCCTGCGC CGCACCCACA CGTTCAACAG CGGCGAGGCC 
CGGCCTGGGG ACCGCCACCG CGGCTGCCAC GCCCGGCCGG GCACAGACTT GGCCCACCTC 
CTCCCCTATG GGGGGGCGGA CAGGACTGCG CCCCCCGTGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA6B ( XM_011527640.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525942.1 Homo sapiens semaphorin 6B (SEMA6B), transcript variant X2, mRNA.

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CloneID	OHu64071
Clone ID Related Accession (Same CDS sequence)	XM_011527639.2 , XM_011527640.2
Accession Version	XM_011527640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGTCCCTGGT CTGCTCTAGG 
AACTGTATCG GCAGTCAGGA CCCCTACTGC GGGTGGGCCC CCGACGGCTC CTGCATCTTC 
CTCAGCCCGG GCACCAGAGC CGCCTTTGAG CAGGACGTGT CCGGGGCCAG CACCTCAGGC 
TTAGGGGACT GCACAGGACT CCTGCGGGCC AGCCTCTCCG AGGACCGCGC GGGGCTGGTG 
TCGGTGAACC TGCTGGTAAC GTCGTCGGTG GCGGCCTTCG TGGTGGGAGC CGTGGTGTCC 
GGCTTCAGCG TGGGCTGGTT CGTGGGCCTC CGTGAGCGGC GGGAGCTGGC CCGGCGCAAG 
GACAAGGAGG CCATCCTGGC GCACGGGGCG GGCGAGGCGG TGCTGAGCGT CAGCCGCCTG 
GGCGAGCGCA GGGCGCAGGG TCCCGGGGGC CGGGGCGGAG GCGGTGGCGG TGGCGCCGGG 
GTTCCCCCGG AGGCCCTGCT GGCGCCCCTG ATGCAGAACG GCTGGGCCAA GGCCACGCTG 
CTGCAGGGCG GGCCCCACGA CCTGGACTCG GGGCTGCTGC CCACGCCCGA GCAGACGCCG 
CTGCCGCAGA AGCGCCTGCC CACTCCGCAC CCGCACCCCC ACGCCCTGGG CCCCCGCGCC 
TGGGACCACG GCCACCCCCT GCTCCCGGCC TCCGCTTCAT CCTCCCTCCT GCTGCTGGCG 
CCCGCCCGGG CCCCCGAGCA GCCCCCCGCG CCTGGGGAGC CGACCCCCGA CGGCCGCCTC 
TATGCTGCCC GGCCCGGCCG CGCCTCCCAC GGCGACTTCC CGCTCACCCC CCACGCCAGC 
CCGGACCGCC GGCGGGTGGT GTCCGCGCCC ACGGGCCCCT TGGACCCAGC CTCAGCCGCC 
GATGGCCTCC CGCGGCCCTG GAGCCCGCCC CCGACGGGCA GCCTGAGGAG GCCACTGGGC 
CCCCACGCCC CTCCGGCCGC CACCCTGCGC CGCACCCACA CGTTCAACAG CGGCGAGGCC 
CGGCCTGGGG ACCGCCACCG CGGCTGCCAC GCCCGGCCGG GCACAGACTT GGCCCACCTC 
CTCCCCTATG GGGGGGCGGA CAGGACTGCG CCCCCCGTGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525942.1
CDS	55..2739
Translation	MQTPRASPPRPALLLLLLLLGGAHGLFPEEPPPLSVAPRDYLNHYPVFVGSGPGRLTPAEGADDLNIQRVLRVNRTLFIGDRDNLYRVELEPPTSTELRYQRKLTWRSNPSDINVCRMKGKQEGECRNFVKVLLLRDESTLFVCGSNAFNPVCANYSIDTLQPVGDNISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRSLGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLEKQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVVLAVFSTPSNSIPGSAVCAFDLTQVAAVFEGRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASSALPDDILNFVKTHPLMDEAVPSLGHAPWILRTLMRHQLTRVAVDVGAGPWGNQTVVFLGSEAGTVLKFLVRPNASTSGTSGLSVFLEEFETYRPDRCGRPGGGETGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQQYSGCMKSLVCSRNCIGSQDPYCGWAPDGSCIFLSPGTRAAFEQDVSGASTSGLGDCTGLLRASLSEDRAGLVSVNLLVTSSVAAFVVGAVVSGFSVGWFVGLRERRELARRKDKEAILAHGAGEAVLSVSRLGERRAQGPGGRGGGGGGGAGVPPEALLAPLMQNGWAKATLLQGGPHDLDSGLLPTPEQTPLPQKRLPTPHPHPHALGPRAWDHGHPLLPASASSSLLLLAPARAPEQPPAPGEPTPDGRLYAARPGRASHGDFPLTPHASPDRRRVVSAPTGPLDPASAADGLPRPWSPPPTGSLRRPLGPHAPPAATLRRTHTFNSGEARPGDRHRGCHARPGTDLAHLLPYGGADRTAPPVP

Target ORF information:

RefSeq Version	XM_011527640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 6B (SEMA6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527640.2

ORF Insert Sequence:

ATGCAGACCC CGCGAGCGTC CCCTCCCCGC CCGGCCCTGC TGCTTCTGCT GCTGCTACTG 
GGGGGCGCCC ACGGCCTCTT TCCTGAGGAG CCGCCGCCGC TTAGCGTGGC CCCCAGGGAC 
TACCTGAACC ACTATCCCGT GTTTGTGGGC AGCGGGCCCG GACGCCTGAC CCCCGCAGAA 
GGTGCTGACG ACCTCAACAT CCAGCGAGTC CTGCGGGTCA ACAGGACGCT GTTCATTGGG 
GACAGGGACA ACCTCTACCG CGTAGAGCTG GAGCCCCCCA CGTCCACGGA GCTGCGGTAC 
CAGAGGAAGC TGACCTGGAG ATCTAACCCC AGCGACATAA ACGTGTGTCG GATGAAGGGC 
AAACAGGAGG GCGAGTGTCG AAACTTCGTA AAGGTGCTGC TCCTTCGGGA CGAGTCCACG 
CTCTTTGTGT GCGGTTCCAA CGCCTTCAAC CCGGTGTGCG CCAACTACAG CATAGACACC 
CTGCAGCCCG TCGGAGACAA CATCAGCGGT ATGGCCCGCT GCCCGTACGA CCCCAAGCAC 
GCCAATGTTG CCCTCTTCTC TGACGGGATG CTCTTCACAG CTACTGTTAC CGACTTCCTA 
GCCATTGATG CTGTCATCTA CCGCAGCCTC GGGGACAGGC CCACCCTGCG CACCGTGAAA 
CATGACTCCA AGTGGTTCAA AGAGCCTTAC TTTGTCCATG CGGTGGAGTG GGGCAGCCAT 
GTCTACTTCT TCTTCCGGGA GATTGCGATG GAGTTTAACT ACCTGGAGAA GGTGGTGGTG 
TCCCGCGTGG CCCGAGTGTG CAAGAACGAC GTGGGAGGCT CCCCCCGCGT GCTGGAGAAG 
CAGTGGACGT CCTTCCTGAA GGCGCGGCTC AACTGCTCTG TACCCGGAGA CTCCCATTTC 
TACTTCAACG TGCTGCAGGC TGTCACGGGC GTGGTCAGCC TCGGGGGCCG GCCCGTGGTC 
CTGGCCGTTT TTTCCACGCC CAGCAACAGC ATCCCTGGCT CGGCTGTCTG CGCCTTTGAC 
CTGACACAGG TGGCAGCTGT GTTTGAAGGC CGCTTCCGAG AGCAGAAGTC CCCCGAGTCC 
ATCTGGACGC CGGTGCCGGA GGATCAGGTG CCTCGACCCC GGCCCGGGTG CTGCGCAGCC 
CCCGGGATGC AGTACAATGC CTCCAGCGCC TTGCCGGATG ACATCCTCAA CTTTGTCAAG 
ACCCACCCTC TGATGGACGA GGCGGTGCCC TCGCTGGGCC ATGCGCCCTG GATCCTGCGG 
ACCCTGATGA GGCACCAGCT GACTCGAGTG GCTGTGGACG TGGGAGCCGG CCCCTGGGGC 
AACCAGACCG TTGTCTTCCT GGGTTCTGAG GCGGGGACGG TCCTCAAGTT CCTCGTCCGG 
CCCAATGCCA GCACCTCAGG GACGTCTGGG CTCAGTGTCT TCCTGGAGGA GTTTGAGACC 
TACCGGCCGG ACAGGTGTGG ACGGCCCGGC GGTGGCGAGA CAGGGCAGCG GCTGCTGAGC 
TTGGAGCTGG ACGCAGCTTC GGGGGGCCTG CTGGCTGCCT TCCCCCGCTG CGTGGTCCGA 
GTGCCTGTGG CTCGCTGCCA GCAGTACTCG GGGTGTATGA AGTCCCTGGT CTGCTCTAGG 
AACTGTATCG GCAGTCAGGA CCCCTACTGC GGGTGGGCCC CCGACGGCTC CTGCATCTTC 
CTCAGCCCGG GCACCAGAGC CGCCTTTGAG CAGGACGTGT CCGGGGCCAG CACCTCAGGC 
TTAGGGGACT GCACAGGACT CCTGCGGGCC AGCCTCTCCG AGGACCGCGC GGGGCTGGTG 
TCGGTGAACC TGCTGGTAAC GTCGTCGGTG GCGGCCTTCG TGGTGGGAGC CGTGGTGTCC 
GGCTTCAGCG TGGGCTGGTT CGTGGGCCTC CGTGAGCGGC GGGAGCTGGC CCGGCGCAAG 
GACAAGGAGG CCATCCTGGC GCACGGGGCG GGCGAGGCGG TGCTGAGCGT CAGCCGCCTG 
GGCGAGCGCA GGGCGCAGGG TCCCGGGGGC CGGGGCGGAG GCGGTGGCGG TGGCGCCGGG 
GTTCCCCCGG AGGCCCTGCT GGCGCCCCTG ATGCAGAACG GCTGGGCCAA GGCCACGCTG 
CTGCAGGGCG GGCCCCACGA CCTGGACTCG GGGCTGCTGC CCACGCCCGA GCAGACGCCG 
CTGCCGCAGA AGCGCCTGCC CACTCCGCAC CCGCACCCCC ACGCCCTGGG CCCCCGCGCC 
TGGGACCACG GCCACCCCCT GCTCCCGGCC TCCGCTTCAT CCTCCCTCCT GCTGCTGGCG 
CCCGCCCGGG CCCCCGAGCA GCCCCCCGCG CCTGGGGAGC CGACCCCCGA CGGCCGCCTC 
TATGCTGCCC GGCCCGGCCG CGCCTCCCAC GGCGACTTCC CGCTCACCCC CCACGCCAGC 
CCGGACCGCC GGCGGGTGGT GTCCGCGCCC ACGGGCCCCT TGGACCCAGC CTCAGCCGCC 
GATGGCCTCC CGCGGCCCTG GAGCCCGCCC CCGACGGGCA GCCTGAGGAG GCCACTGGGC 
CCCCACGCCC CTCCGGCCGC CACCCTGCGC CGCACCCACA CGTTCAACAG CGGCGAGGCC 
CGGCCTGGGG ACCGCCACCG CGGCTGCCAC GCCCGGCCGG GCACAGACTT GGCCCACCTC 
CTCCCCTATG GGGGGGCGGA CAGGACTGCG CCCCCCGTGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of SEMA6B gene expression in breast cancer: identification of a new isoform.
Biochimica et biophysica acta1830(10)4543-53(2013 Oct)
D'Apice L,Costa V,Valente C,Trovato M,Pagani A,Manera S,Regolo L,Zambelli A,Ciccodicola A,De Berardinis P

The role of axon guidance factor semaphorin 6B in the invasion and metastasis of gastric cancer.
The Journal of international medical research41(2)284-92(2013 Apr)
Ge C,Li Q,Wang L,Xu X

Signal peptide prediction based on analysis of experimentally verified cleavage sites.
Protein science : a publication of the Protein Society13(10)2819-24(2004 Oct)
Zhang Z,Henzel WJ

The human semaphorin 6B gene is down regulated by PPARs.
Genomics83(6)1141-50(2004 Jun)
Collet P,Domenjoud L,Devignes MD,Murad H,Schohn H,Dau?a M

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

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SEMA6B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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