CLGN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLGN
Entrez Gene ID	1047
Full Name	calmegin
General protein information	Preferred Names calmegin Names calmegin testis tissue sperm-binding protein Li 79P
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q31.1
Summary	Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq, Jul 2008].
Disorder MIM:	601858

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130675.1	NP_001124147.1	calmegin precursor
NM_004362.3	NP_004353.1	calmegin precursor
NM_004362.2	NP_004353.1	calmegin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLGN	XP_003310574.1
Danio rerio (zebrafish)	clgn	NP_001071009.1
Rattus norvegicus (Norway rat)	Clgn	NP_001102942.1
Neurospora crassa	NCU09265	XP_964700.2
Homo sapiens (human)	CLGN	NP_004353.1
Bos taurus (cattle)	CLGN	NP_001029377.1
	CLGN	XP_001089882.1
Mus musculus (house mouse)	Clgn	NP_034034.2
Xenopus tropicalis (tropical clawed frog)	clgn	XP_002932081.2
Canis lupus familiaris (dog)	CLGN	XP_533285.3
Gallus gallus (chicken)	CLGN	XP_420413.4
Schizosaccharomyces pombe (fission yeast)	cnx1	NP_593612.1
Magnaporthe oryzae (rice blast fungus)	MGG_01607	XP_003714591.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-Wide Gene-Sodium Interaction Analyses on Blood Pressure: The Genetic Epidemiology Network of Salt-Sensitivity Study.
Li C, He J, Chen J, Zhao J, Gu D, Hixson JE, Rao DC, Jaquish CE, Gu CC, Chen J, Huang J, Chen S, Kelly TN
Hypertension (Dallas, Tex. : 1979)68(2)348-55(2016 08)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Gupta GD, Coyaud ?, Gon?alves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L
Cell163(6)1484-99(2015 Dec)

Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions.
Sakono M, Seko A, Takeda Y, Aikawa J, Hachisu M, Koizumi A, Fujikawa K, Ito Y
Biochimica et biophysica acta1840(9)2904-13(2014 Sep)

Genome-wide association with select biomarker traits in the Framingham Heart Study.
Benjamin EJ, Dupuis J, Larson MG, Lunetta KL, Booth SL, Govindaraju DR, Kathiresan S, Keaney JF Jr, Keyes MJ, Lin JP, Meigs JB, Robins SJ, Rong J, Schnabel R, Vita JA, Wang TJ, Wilson PW, Wolf PA, Vasan RS
BMC medical genetics8 Suppl 1S11(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In gene-based analysis CLGN1 represented one of a cluster of genes, which interacted with sodium to influence Blood Pressure.
Title: Genome-Wide Gene-Sodium Interaction Analyses on Blood Pressure: The Genetic Epidemiology Network of Salt-Sensitivity Study.

Although their similarity in carbohydrate binding specificities is high, there seems to be some differences in the mode of substrate recognition between calmegin and calnexin.
Title: Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

calmegin expression is regulated by histone deacetylase and CpG methyltransferase in a coordinative way
Title: Coordinated transcriptional regulation of calmegin,a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase.

The following CLGN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLGN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16511	NM_001130675.1 Latest version!	Homo sapiens calmegin (CLGN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16511	NM_004362.3 Latest version!	Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu16511	NM_004362.2	Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLGN ( NM_001130675.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124147.1 Homo sapiens calmegin (CLGN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16511
Clone ID Related Accession (Same CDS sequence)	NM_001130675.1 , NM_004362.3 , NM_004362.2
Accession Version	NM_001130675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	calmegin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC396076.1, AL045602.1, BC028357.2 and D86322.1. Transcript Variant: This variant (2) contains an extra exon in the 5' UTR, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005702.1, SRR1660805.28588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCATTTCC AAGCCTTTTG GCTATGTTTG GGTCTTCTGT TCATCTCAAT TAATGCAGAA 
TTTATGGATG ATGATGTTGA GACGGAAGAC TTTGAAGAAA ATTCAGAAGA AATTGATGTT 
AATGAAAGTG AACTTTCCTC AGAGATTAAA TATAAGACAC CTCAACCTAT AGGAGAAGTA 
TATTTTGCAG AAACTTTTGA TAGTGGAAGG TTGGCTGGAT GGGTCTTATC AAAAGCAAAG 
AAAGATGACA TGGATGAGGA AATTTCAATA TACGATGGAA GATGGGAAAT TGAAGAGTTG 
AAAGAAAACC AGGTACCTGG TGACAGAGGA CTGGTATTAA AATCTAGAGC AAAGCATCAT 
GCAATATCTG CTGTATTAGC AAAACCATTC ATTTTTGCTG ATAAACCCTT GATAGTTCAA 
TATGAAGTAA ATTTTCAAGA TGGTATTGAT TGTGGAGGTG CATACATTAA ACTCCTAGCA 
GACACTGATG ATTTGATTCT GGAAAACTTT TATGATAAAA CATCCTATAT CATTATGTTT 
GGACCAGATA AATGTGGAGA AGATTATAAA CTTCATTTTA TCTTCAGACA TAAACATCCC 
AAAACTGGAG TTTTCGAAGA GAAACATGCC AAACCTCCAG ATGTAGACCT TAAAAAGTTC 
TTTACAGACA GGAAGACTCA TCTTTATACC CTTGTGATGA ATCCAGATGA CACATTTGAG 
GTGTTAGTTG ATCAAACAGT TGTAAACAAA GGAAGCCTCC TAGAGGATGT GGTTCCTCCT 
ATCAAACCTC CCAAAGAAAT TGAAGATCCC AATGATAAAA AACCTGAGGA ATGGGATGAA 
AGAGCAAAAA TTCCTGATCC TTCTGCCGTC AAACCAGAAG ACTGGGATGA AAGTGAACCT 
GCCCAAATAG AAGATTCAAG TGTTGTTAAA CCTGCTGGCT GGCTTGATGA TGAACCAAAA 
TTTATCCCTG ATCCTAATGC TGAAAAACCT GATGACTGGA ATGAAGACAC GGATGGAGAA 
TGGGAGGCAC CTCAGATTCT TAATCCAGCA TGTCGGATTG GGTGTGGTGA GTGGAAACCT 
CCCATGATAG ATAACCCAAA ATACAAAGGA GTATGGAGAC CTCCACTGGT CGATAATCCT 
AACTATCAGG GAATCTGGAG TCCTCGAAAA ATTCCTAATC CAGATTATTT CGAAGATGAT 
CATCCATTTC TTCTGACTTC TTTCAGTGCT CTTGGTTTAG AGCTTTGGTC TATGACCTCT 
GATATCTACT TTGATAATTT TATTATCTGT TCGGAAAAGG AAGTAGCAGA TCACTGGGCT 
GCAGATGGTT GGAGATGGAA AATAATGATA GCAAATGCTA ATAAGCCTGG TGTATTAAAA 
CAGTTAATGG CAGCTGCTGA AGGGCACCCA TGGCTTTGGT TGATTTATCT TGTGACAGCA 
GGAGTGCCAA TAGCATTAAT TACTTCATTT TGTTGGCCAA GAAAAGTAAA GAAAAAACAT 
AAAGATACAG AGTATAAAAA AACCGACATA TGTATACCAC AAACAAAAGG AGTACTAGAG 
CAAGAAGAAA AGGAAGAGAA AGCAGCCCTG GAAAAACCAA TGGACCTGGA AGAGGAAAAA 
AAGCAAAATG ATGGTGAAAT GCTTGAAAAA GAAGAGGAAA GTGAACCTGA GGAAAAGAGT 
GAAGAAGAAA TTGAAATCAT AGAAGGGCAA GAAGAAAGTA ATCAATCAAA TAAGTCTGGG 
TCAGAGGATG AGATGAAAGA AGCAGATGAG AGCACAGGAT CTGGAGATGG GCCGATAAAG 
TCAGTACGCA AAAGAAGAGT ACGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124147.1
CDS	219..2051
Translation	MHFQAFWLCLGLLFISINAEFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD

Target ORF information:

RefSeq Version	NM_001130675.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calmegin (CLGN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130675.1

ORF Insert Sequence:

ATGCATTTCC AAGCCTTTTG GCTATGTTTG GGTCTTCTGT TCATCTCAAT TAATGCAGAA 
TTTATGGATG ATGATGTTGA GACGGAAGAC TTTGAAGAAA ATTCAGAAGA AATTGATGTT 
AATGAAAGTG AACTTTCCTC AGAGATTAAA TATAAGACAC CTCAACCTAT AGGAGAAGTA 
TATTTTGCAG AAACTTTTGA TAGTGGAAGG TTGGCTGGAT GGGTCTTATC AAAAGCAAAG 
AAAGATGACA TGGATGAGGA AATTTCAATA TACGATGGAA GATGGGAAAT TGAAGAGTTG 
AAAGAAAACC AGGTACCTGG TGACAGAGGA CTGGTATTAA AATCTAGAGC AAAGCATCAT 
GCAATATCTG CTGTATTAGC AAAACCATTC ATTTTTGCTG ATAAACCCTT GATAGTTCAA 
TATGAAGTAA ATTTTCAAGA TGGTATTGAT TGTGGAGGTG CATACATTAA ACTCCTAGCA 
GACACTGATG ATTTGATTCT GGAAAACTTT TATGATAAAA CATCCTATAT CATTATGTTT 
GGACCAGATA AATGTGGAGA AGATTATAAA CTTCATTTTA TCTTCAGACA TAAACATCCC 
AAAACTGGAG TTTTCGAAGA GAAACATGCC AAACCTCCAG ATGTAGACCT TAAAAAGTTC 
TTTACAGACA GGAAGACTCA TCTTTATACC CTTGTGATGA ATCCAGATGA CACATTTGAG 
GTGTTAGTTG ATCAAACAGT TGTAAACAAA GGAAGCCTCC TAGAGGATGT GGTTCCTCCT 
ATCAAACCTC CCAAAGAAAT TGAAGATCCC AATGATAAAA AACCTGAGGA ATGGGATGAA 
AGAGCAAAAA TTCCTGATCC TTCTGCCGTC AAACCAGAAG ACTGGGATGA AAGTGAACCT 
GCCCAAATAG AAGATTCAAG TGTTGTTAAA CCTGCTGGCT GGCTTGATGA TGAACCAAAA 
TTTATCCCTG ATCCTAATGC TGAAAAACCT GATGACTGGA ATGAAGACAC GGATGGAGAA 
TGGGAGGCAC CTCAGATTCT TAATCCAGCA TGTCGGATTG GGTGTGGTGA GTGGAAACCT 
CCCATGATAG ATAACCCAAA ATACAAAGGA GTATGGAGAC CTCCACTGGT CGATAATCCT 
AACTATCAGG GAATCTGGAG TCCTCGAAAA ATTCCTAATC CAGATTATTT CGAAGATGAT 
CATCCATTTC TTCTGACTTC TTTCAGTGCT CTTGGTTTAG AGCTTTGGTC TATGACCTCT 
GATATCTACT TTGATAATTT TATTATCTGT TCGGAAAAGG AAGTAGCAGA TCACTGGGCT 
GCAGATGGTT GGAGATGGAA AATAATGATA GCAAATGCTA ATAAGCCTGG TGTATTAAAA 
CAGTTAATGG CAGCTGCTGA AGGGCACCCA TGGCTTTGGT TGATTTATCT TGTGACAGCA 
GGAGTGCCAA TAGCATTAAT TACTTCATTT TGTTGGCCAA GAAAAGTAAA GAAAAAACAT 
AAAGATACAG AGTATAAAAA AACCGACATA TGTATACCAC AAACAAAAGG AGTACTAGAG 
CAAGAAGAAA AGGAAGAGAA AGCAGCCCTG GAAAAACCAA TGGACCTGGA AGAGGAAAAA 
AAGCAAAATG ATGGTGAAAT GCTTGAAAAA GAAGAGGAAA GTGAACCTGA GGAAAAGAGT 
GAAGAAGAAA TTGAAATCAT AGAAGGGCAA GAAGAAAGTA ATCAATCAAA TAAGTCTGGG 
TCAGAGGATG AGATGAAAGA AGCAGATGAG AGCACAGGAT CTGGAGATGG GCCGATAAAG 
TCAGTACGCA AAAGAAGAGT ACGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLGN ( NM_004362.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004353.1 Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16511
Clone ID Related Accession (Same CDS sequence)	NM_001130675.1 , NM_004362.3 , NM_004362.2
Accession Version	NM_004362.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	calmegin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC396076.1, BC028357.2 and D86322.1. On Nov 22, 2018 this sequence version replaced NM_004362.2. Transcript Variant: This variant (1) is the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86322.1, SRR1803617.222683.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325617.10/ ENSP00000326699.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCATTTCC AAGCCTTTTG GCTATGTTTG GGTCTTCTGT TCATCTCAAT TAATGCAGAA 
TTTATGGATG ATGATGTTGA GACGGAAGAC TTTGAAGAAA ATTCAGAAGA AATTGATGTT 
AATGAAAGTG AACTTTCCTC AGAGATTAAA TATAAGACAC CTCAACCTAT AGGAGAAGTA 
TATTTTGCAG AAACTTTTGA TAGTGGAAGG TTGGCTGGAT GGGTCTTATC AAAAGCAAAG 
AAAGATGACA TGGATGAGGA AATTTCAATA TACGATGGAA GATGGGAAAT TGAAGAGTTG 
AAAGAAAACC AGGTACCTGG TGACAGAGGA CTGGTATTAA AATCTAGAGC AAAGCATCAT 
GCAATATCTG CTGTATTAGC AAAACCATTC ATTTTTGCTG ATAAACCCTT GATAGTTCAA 
TATGAAGTAA ATTTTCAAGA TGGTATTGAT TGTGGAGGTG CATACATTAA ACTCCTAGCA 
GACACTGATG ATTTGATTCT GGAAAACTTT TATGATAAAA CATCCTATAT CATTATGTTT 
GGACCAGATA AATGTGGAGA AGATTATAAA CTTCATTTTA TCTTCAGACA TAAACATCCC 
AAAACTGGAG TTTTCGAAGA GAAACATGCC AAACCTCCAG ATGTAGACCT TAAAAAGTTC 
TTTACAGACA GGAAGACTCA TCTTTATACC CTTGTGATGA ATCCAGATGA CACATTTGAG 
GTGTTAGTTG ATCAAACAGT TGTAAACAAA GGAAGCCTCC TAGAGGATGT GGTTCCTCCT 
ATCAAACCTC CCAAAGAAAT TGAAGATCCC AATGATAAAA AACCTGAGGA ATGGGATGAA 
AGAGCAAAAA TTCCTGATCC TTCTGCCGTC AAACCAGAAG ACTGGGATGA AAGTGAACCT 
GCCCAAATAG AAGATTCAAG TGTTGTTAAA CCTGCTGGCT GGCTTGATGA TGAACCAAAA 
TTTATCCCTG ATCCTAATGC TGAAAAACCT GATGACTGGA ATGAAGACAC GGATGGAGAA 
TGGGAGGCAC CTCAGATTCT TAATCCAGCA TGTCGGATTG GGTGTGGTGA GTGGAAACCT 
CCCATGATAG ATAACCCAAA ATACAAAGGA GTATGGAGAC CTCCACTGGT CGATAATCCT 
AACTATCAGG GAATCTGGAG TCCTCGAAAA ATTCCTAATC CAGATTATTT CGAAGATGAT 
CATCCATTTC TTCTGACTTC TTTCAGTGCT CTTGGTTTAG AGCTTTGGTC TATGACCTCT 
GATATCTACT TTGATAATTT TATTATCTGT TCGGAAAAGG AAGTAGCAGA TCACTGGGCT 
GCAGATGGTT GGAGATGGAA AATAATGATA GCAAATGCTA ATAAGCCTGG TGTATTAAAA 
CAGTTAATGG CAGCTGCTGA AGGGCACCCA TGGCTTTGGT TGATTTATCT TGTGACAGCA 
GGAGTGCCAA TAGCATTAAT TACTTCATTT TGTTGGCCAA GAAAAGTAAA GAAAAAACAT 
AAAGATACAG AGTATAAAAA AACCGACATA TGTATACCAC AAACAAAAGG AGTACTAGAG 
CAAGAAGAAA AGGAAGAGAA AGCAGCCCTG GAAAAACCAA TGGACCTGGA AGAGGAAAAA 
AAGCAAAATG ATGGTGAAAT GCTTGAAAAA GAAGAGGAAA GTGAACCTGA GGAAAAGAGT 
GAAGAAGAAA TTGAAATCAT AGAAGGGCAA GAAGAAAGTA ATCAATCAAA TAAGTCTGGG 
TCAGAGGATG AGATGAAAGA AGCAGATGAG AGCACAGGAT CTGGAGATGG GCCGATAAAG 
TCAGTACGCA AAAGAAGAGT ACGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004353.1
CDS	122..1954
Translation	MHFQAFWLCLGLLFISINAEFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD

Target ORF information:

RefSeq Version	NM_004362.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004362.3

ORF Insert Sequence:

ATGCATTTCC AAGCCTTTTG GCTATGTTTG GGTCTTCTGT TCATCTCAAT TAATGCAGAA 
TTTATGGATG ATGATGTTGA GACGGAAGAC TTTGAAGAAA ATTCAGAAGA AATTGATGTT 
AATGAAAGTG AACTTTCCTC AGAGATTAAA TATAAGACAC CTCAACCTAT AGGAGAAGTA 
TATTTTGCAG AAACTTTTGA TAGTGGAAGG TTGGCTGGAT GGGTCTTATC AAAAGCAAAG 
AAAGATGACA TGGATGAGGA AATTTCAATA TACGATGGAA GATGGGAAAT TGAAGAGTTG 
AAAGAAAACC AGGTACCTGG TGACAGAGGA CTGGTATTAA AATCTAGAGC AAAGCATCAT 
GCAATATCTG CTGTATTAGC AAAACCATTC ATTTTTGCTG ATAAACCCTT GATAGTTCAA 
TATGAAGTAA ATTTTCAAGA TGGTATTGAT TGTGGAGGTG CATACATTAA ACTCCTAGCA 
GACACTGATG ATTTGATTCT GGAAAACTTT TATGATAAAA CATCCTATAT CATTATGTTT 
GGACCAGATA AATGTGGAGA AGATTATAAA CTTCATTTTA TCTTCAGACA TAAACATCCC 
AAAACTGGAG TTTTCGAAGA GAAACATGCC AAACCTCCAG ATGTAGACCT TAAAAAGTTC 
TTTACAGACA GGAAGACTCA TCTTTATACC CTTGTGATGA ATCCAGATGA CACATTTGAG 
GTGTTAGTTG ATCAAACAGT TGTAAACAAA GGAAGCCTCC TAGAGGATGT GGTTCCTCCT 
ATCAAACCTC CCAAAGAAAT TGAAGATCCC AATGATAAAA AACCTGAGGA ATGGGATGAA 
AGAGCAAAAA TTCCTGATCC TTCTGCCGTC AAACCAGAAG ACTGGGATGA AAGTGAACCT 
GCCCAAATAG AAGATTCAAG TGTTGTTAAA CCTGCTGGCT GGCTTGATGA TGAACCAAAA 
TTTATCCCTG ATCCTAATGC TGAAAAACCT GATGACTGGA ATGAAGACAC GGATGGAGAA 
TGGGAGGCAC CTCAGATTCT TAATCCAGCA TGTCGGATTG GGTGTGGTGA GTGGAAACCT 
CCCATGATAG ATAACCCAAA ATACAAAGGA GTATGGAGAC CTCCACTGGT CGATAATCCT 
AACTATCAGG GAATCTGGAG TCCTCGAAAA ATTCCTAATC CAGATTATTT CGAAGATGAT 
CATCCATTTC TTCTGACTTC TTTCAGTGCT CTTGGTTTAG AGCTTTGGTC TATGACCTCT 
GATATCTACT TTGATAATTT TATTATCTGT TCGGAAAAGG AAGTAGCAGA TCACTGGGCT 
GCAGATGGTT GGAGATGGAA AATAATGATA GCAAATGCTA ATAAGCCTGG TGTATTAAAA 
CAGTTAATGG CAGCTGCTGA AGGGCACCCA TGGCTTTGGT TGATTTATCT TGTGACAGCA 
GGAGTGCCAA TAGCATTAAT TACTTCATTT TGTTGGCCAA GAAAAGTAAA GAAAAAACAT 
AAAGATACAG AGTATAAAAA AACCGACATA TGTATACCAC AAACAAAAGG AGTACTAGAG 
CAAGAAGAAA AGGAAGAGAA AGCAGCCCTG GAAAAACCAA TGGACCTGGA AGAGGAAAAA 
AAGCAAAATG ATGGTGAAAT GCTTGAAAAA GAAGAGGAAA GTGAACCTGA GGAAAAGAGT 
GAAGAAGAAA TTGAAATCAT AGAAGGGCAA GAAGAAAGTA ATCAATCAAA TAAGTCTGGG 
TCAGAGGATG AGATGAAAGA AGCAGATGAG AGCACAGGAT CTGGAGATGG GCCGATAAAG 
TCAGTACGCA AAAGAAGAGT ACGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLGN ( NM_004362.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004353.1 Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16511
Clone ID Related Accession (Same CDS sequence)	NM_001130675.1 , NM_004362.3 , NM_004362.2
Accession Version	NM_004362.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	calmegin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC396076.1, BC028357.2 and D86322.1. On Jul 29, 2008 this sequence version replaced NM_004362.1. Transcript Variant: This variant (1) is the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86322.1, SRR1803617.222683.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATTTCC AAGCCTTTTG GCTATGTTTG GGTCTTCTGT TCATCTCAAT TAATGCAGAA 
TTTATGGATG ATGATGTTGA GACGGAAGAC TTTGAAGAAA ATTCAGAAGA AATTGATGTT 
AATGAAAGTG AACTTTCCTC AGAGATTAAA TATAAGACAC CTCAACCTAT AGGAGAAGTA 
TATTTTGCAG AAACTTTTGA TAGTGGAAGG TTGGCTGGAT GGGTCTTATC AAAAGCAAAG 
AAAGATGACA TGGATGAGGA AATTTCAATA TACGATGGAA GATGGGAAAT TGAAGAGTTG 
AAAGAAAACC AGGTACCTGG TGACAGAGGA CTGGTATTAA AATCTAGAGC AAAGCATCAT 
GCAATATCTG CTGTATTAGC AAAACCATTC ATTTTTGCTG ATAAACCCTT GATAGTTCAA 
TATGAAGTAA ATTTTCAAGA TGGTATTGAT TGTGGAGGTG CATACATTAA ACTCCTAGCA 
GACACTGATG ATTTGATTCT GGAAAACTTT TATGATAAAA CATCCTATAT CATTATGTTT 
GGACCAGATA AATGTGGAGA AGATTATAAA CTTCATTTTA TCTTCAGACA TAAACATCCC 
AAAACTGGAG TTTTCGAAGA GAAACATGCC AAACCTCCAG ATGTAGACCT TAAAAAGTTC 
TTTACAGACA GGAAGACTCA TCTTTATACC CTTGTGATGA ATCCAGATGA CACATTTGAG 
GTGTTAGTTG ATCAAACAGT TGTAAACAAA GGAAGCCTCC TAGAGGATGT GGTTCCTCCT 
ATCAAACCTC CCAAAGAAAT TGAAGATCCC AATGATAAAA AACCTGAGGA ATGGGATGAA 
AGAGCAAAAA TTCCTGATCC TTCTGCCGTC AAACCAGAAG ACTGGGATGA AAGTGAACCT 
GCCCAAATAG AAGATTCAAG TGTTGTTAAA CCTGCTGGCT GGCTTGATGA TGAACCAAAA 
TTTATCCCTG ATCCTAATGC TGAAAAACCT GATGACTGGA ATGAAGACAC GGATGGAGAA 
TGGGAGGCAC CTCAGATTCT TAATCCAGCA TGTCGGATTG GGTGTGGTGA GTGGAAACCT 
CCCATGATAG ATAACCCAAA ATACAAAGGA GTATGGAGAC CTCCACTGGT CGATAATCCT 
AACTATCAGG GAATCTGGAG TCCTCGAAAA ATTCCTAATC CAGATTATTT CGAAGATGAT 
CATCCATTTC TTCTGACTTC TTTCAGTGCT CTTGGTTTAG AGCTTTGGTC TATGACCTCT 
GATATCTACT TTGATAATTT TATTATCTGT TCGGAAAAGG AAGTAGCAGA TCACTGGGCT 
GCAGATGGTT GGAGATGGAA AATAATGATA GCAAATGCTA ATAAGCCTGG TGTATTAAAA 
CAGTTAATGG CAGCTGCTGA AGGGCACCCA TGGCTTTGGT TGATTTATCT TGTGACAGCA 
GGAGTGCCAA TAGCATTAAT TACTTCATTT TGTTGGCCAA GAAAAGTAAA GAAAAAACAT 
AAAGATACAG AGTATAAAAA AACCGACATA TGTATACCAC AAACAAAAGG AGTACTAGAG 
CAAGAAGAAA AGGAAGAGAA AGCAGCCCTG GAAAAACCAA TGGACCTGGA AGAGGAAAAA 
AAGCAAAATG ATGGTGAAAT GCTTGAAAAA GAAGAGGAAA GTGAACCTGA GGAAAAGAGT 
GAAGAAGAAA TTGAAATCAT AGAAGGGCAA GAAGAAAGTA ATCAATCAAA TAAGTCTGGG 
TCAGAGGATG AGATGAAAGA AGCAGATGAG AGCACAGGAT CTGGAGATGG GCCGATAAAG 
TCAGTACGCA AAAGAAGAGT ACGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004353.1
CDS	135..1967
Translation	MHFQAFWLCLGLLFISINAEFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD

Target ORF information:

RefSeq Version	NM_004362.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calmegin (CLGN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004362.2

ORF Insert Sequence:

ATGCATTTCC AAGCCTTTTG GCTATGTTTG GGTCTTCTGT TCATCTCAAT TAATGCAGAA 
TTTATGGATG ATGATGTTGA GACGGAAGAC TTTGAAGAAA ATTCAGAAGA AATTGATGTT 
AATGAAAGTG AACTTTCCTC AGAGATTAAA TATAAGACAC CTCAACCTAT AGGAGAAGTA 
TATTTTGCAG AAACTTTTGA TAGTGGAAGG TTGGCTGGAT GGGTCTTATC AAAAGCAAAG 
AAAGATGACA TGGATGAGGA AATTTCAATA TACGATGGAA GATGGGAAAT TGAAGAGTTG 
AAAGAAAACC AGGTACCTGG TGACAGAGGA CTGGTATTAA AATCTAGAGC AAAGCATCAT 
GCAATATCTG CTGTATTAGC AAAACCATTC ATTTTTGCTG ATAAACCCTT GATAGTTCAA 
TATGAAGTAA ATTTTCAAGA TGGTATTGAT TGTGGAGGTG CATACATTAA ACTCCTAGCA 
GACACTGATG ATTTGATTCT GGAAAACTTT TATGATAAAA CATCCTATAT CATTATGTTT 
GGACCAGATA AATGTGGAGA AGATTATAAA CTTCATTTTA TCTTCAGACA TAAACATCCC 
AAAACTGGAG TTTTCGAAGA GAAACATGCC AAACCTCCAG ATGTAGACCT TAAAAAGTTC 
TTTACAGACA GGAAGACTCA TCTTTATACC CTTGTGATGA ATCCAGATGA CACATTTGAG 
GTGTTAGTTG ATCAAACAGT TGTAAACAAA GGAAGCCTCC TAGAGGATGT GGTTCCTCCT 
ATCAAACCTC CCAAAGAAAT TGAAGATCCC AATGATAAAA AACCTGAGGA ATGGGATGAA 
AGAGCAAAAA TTCCTGATCC TTCTGCCGTC AAACCAGAAG ACTGGGATGA AAGTGAACCT 
GCCCAAATAG AAGATTCAAG TGTTGTTAAA CCTGCTGGCT GGCTTGATGA TGAACCAAAA 
TTTATCCCTG ATCCTAATGC TGAAAAACCT GATGACTGGA ATGAAGACAC GGATGGAGAA 
TGGGAGGCAC CTCAGATTCT TAATCCAGCA TGTCGGATTG GGTGTGGTGA GTGGAAACCT 
CCCATGATAG ATAACCCAAA ATACAAAGGA GTATGGAGAC CTCCACTGGT CGATAATCCT 
AACTATCAGG GAATCTGGAG TCCTCGAAAA ATTCCTAATC CAGATTATTT CGAAGATGAT 
CATCCATTTC TTCTGACTTC TTTCAGTGCT CTTGGTTTAG AGCTTTGGTC TATGACCTCT 
GATATCTACT TTGATAATTT TATTATCTGT TCGGAAAAGG AAGTAGCAGA TCACTGGGCT 
GCAGATGGTT GGAGATGGAA AATAATGATA GCAAATGCTA ATAAGCCTGG TGTATTAAAA 
CAGTTAATGG CAGCTGCTGA AGGGCACCCA TGGCTTTGGT TGATTTATCT TGTGACAGCA 
GGAGTGCCAA TAGCATTAAT TACTTCATTT TGTTGGCCAA GAAAAGTAAA GAAAAAACAT 
AAAGATACAG AGTATAAAAA AACCGACATA TGTATACCAC AAACAAAAGG AGTACTAGAG 
CAAGAAGAAA AGGAAGAGAA AGCAGCCCTG GAAAAACCAA TGGACCTGGA AGAGGAAAAA 
AAGCAAAATG ATGGTGAAAT GCTTGAAAAA GAAGAGGAAA GTGAACCTGA GGAAAAGAGT 
GAAGAAGAAA TTGAAATCAT AGAAGGGCAA GAAGAAAGTA ATCAATCAAA TAAGTCTGGG 
TCAGAGGATG AGATGAAAGA AGCAGATGAG AGCACAGGAT CTGGAGATGG GCCGATAAAG 
TCAGTACGCA AAAGAAGAGT ACGAAAGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-Wide Gene-Sodium Interaction Analyses on Blood Pressure: The Genetic Epidemiology Network of Salt-Sensitivity Study.
Hypertension (Dallas, Tex. : 1979)68(2)348-55(2016 08)
Li C,He J,Chen J,Zhao J,Gu D,Hixson JE,Rao DC,Jaquish CE,Gu CC,Chen J,Huang J,Chen S,Kelly TN

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Cell163(6)1484-99(2015 Dec)
Gupta GD,Coyaud ?,Gon?alves J,Mojarad BA,Liu Y,Wu Q,Gheiratmand L,Comartin D,Tkach JM,Cheung SW,Bashkurov M,Hasegan M,Knight JD,Lin ZY,Schueler M,Hildebrandt F,Moffat J,Gingras AC,Raught B,Pelletier L

Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions.
Biochimica et biophysica acta1840(9)2904-13(2014 Sep)
Sakono M,Seko A,Takeda Y,Aikawa J,Hachisu M,Koizumi A,Fujikawa K,Ito Y

Genome-wide association with select biomarker traits in the Framingham Heart Study.
BMC medical genetics8 Suppl 1S11(2007 Sep)
Benjamin EJ,Dupuis J,Larson MG,Lunetta KL,Booth SL,Govindaraju DR,Kathiresan S,Keaney JF Jr,Keyes MJ,Lin JP,Meigs JB,Robins SJ,Rong J,Schnabel R,Vita JA,Wang TJ,Wilson PW,Wolf PA,Vasan RS

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CLGN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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