APC2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	APC2
Entrez Gene ID	10297
Full Name	APC2, WNT signaling pathway regulator
Synonyms	APCL
General protein information	Preferred Names APC2, WNT signaling pathway regulator Names adenomatous polyposis coli protein 2 adenomatosis polyposis coli 2 adenomatous polyposis coli like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a strongly conserved protein that has an N-terminal coiled-coil domain followed by an armadillo domain, five 20-amino acid repeats, and two SAMP domains. This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. The protein encoded by this gene is closely related to the adenomatous polyposis coli (APC) tumor-suppressor protein and has similar tumor-suppressor effects. This gene also plays a role in actin assembly, cell-cell adhesion, and microtubule network formation through its interaction with cytoskeletal proteins. This gene has its highest expression in the central nervous system and is involved in brain development through cytoskeletal regulation in neurons. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017].
Disorder MIM:	612034
Disorder Html:	?Sotos syndrome 3, 617169 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001351273.1	NP_001338202.1	adenomatous polyposis coli protein 2 isoform 2
XM_006722608.3	XP_006722671.2	adenomatous polyposis coli protein 2 isoform X1
XM_005259475.2	XP_005259532.1	adenomatous polyposis coli protein 2 isoform X3
NM_005883.2	NP_005874.1	adenomatous polyposis coli protein 2 isoform 1
XM_006722610.3	XP_006722673.2	adenomatous polyposis coli protein 2 isoform X2
XM_006722607.2	XP_006722670.1	adenomatous polyposis coli protein 2 isoform X4
XM_006722609.3	XP_006722672.1	adenomatous polyposis coli protein 2 isoform X5

Pathways from BioSystems

Homologies

Homo sapiens (human)	APC2	NP_005874.1
Pan troglodytes (chimpanzee)	APC2	XP_003953359.1
Bos taurus (cattle)	APC2	XP_002689221.3
Danio rerio (zebrafish)	apc2	XP_694133.6
Xenopus tropicalis (tropical clawed frog)	apc2	XP_002941166.2
	APC2	XP_001095216.2
Canis lupus familiaris (dog)	APC2	XP_542202.4
Mus musculus (house mouse)	Apc2	NP_035919.2
Rattus norvegicus (Norway rat)	Apc2	NP_001100239.2
Gallus gallus (chicken)	APC2	NP_001165242.1

Gene Ontology
Bibliography

Related articles in PubMed

Hsa-miR-11181 regulates Wnt signaling pathway through targeting of APC2 transcripts in SW480 cell line.
Dokanehiifard S, Soltani BM
Gene641297-302(2018 Jan)

Familial adenomatous polyposis in children younger than age ten years: a multidisciplinary clinic experience.
Attard TM, Tajouri T, Peterson KD, Tinley S, Thorson AG, Lynch HT
Diseases of the colon and rectum51(2)207-12(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

APC2 3'-UTR is targeted by hsa-miR-11181.
Title: Hsa-miR-11181 regulates Wnt signaling pathway through targeting of APC2 transcripts in SW480 cell line.

Functional redundancy between Apc and Apc2 regulates tissue homeostasis and prevents tumorigenesis in murine mammary epithelium
Title: Functional redundancy between Apc and Apc2 regulates tissue homeostasis and prevents tumorigenesis in murine mammary epithelium.

Data show that poly(ADP-ribose) polymerase (PARP) enzyme Tankyrase (TNKS) inhibition in colon cancer cells decreases beta-catenin signaling at the level of both Axin and APC2.
Title: The Poly(ADP-ribose) Polymerase Enzyme Tankyrase Antagonizes Activity of the ?-Catenin Destruction Complex through ADP-ribosylation of Axin and APC2.

We report that APC-2 gene is hypermethylated in both RB tumor samples and Y79 cells. Reduced APC-2 lead to increased Wnt signaling pathway protein, beta-catenin suggesting tumor suppressive role of APC-2 gene.
Title: Hypermethylation of adenomatosis polyposis coli-2 and its tumor suppressor role in retinoblastoma.

miR-939 functioned as a potential tumor promoter by regulating the Wnt/beta-catenin signal pathway through direct suppression of APC2 expression.
Title: MiR-939 promotes the proliferation of human ovarian cancer cells by repressing APC2 expression.

The following APC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05000	NM_005883.2 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu51394	NM_001351273.1 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu05000	XM_006722609.3 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu51392	XM_005259475.2 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu51393	XM_006722607.2 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu88116	XM_006722608.3 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu88117	XM_006722610.3 Latest version!	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APC2 ( NM_005883.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005874.1 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05000
Clone ID Related Accession (Same CDS sequence)	NM_005883.2 , XM_006722609.3
Accession Version	NM_005883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032573.1 and AC027307.5. This sequence is a reference standard in the RefSeqGene project. On Jan 7, 2005 this sequence version replaced NM_005883.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAGCT CCGTGGCGCC CTACGAGCAG CTGGTGAGGC AGGTGGAGGC CTTGAAGGCT 
GAGAACAGCC ACCTGAGGCA GGAGCTAAGG GACAACTCCA GCCACCTGTC CAAGCTGGAG 
ACAGAGACGT CGGGCATGAA GGAGGTCCTG AAGCACCTAC AGGGAAAACT GGAGCAGGAG 
GCCCGAGTGC TGGTGTCCTC GGGGCAGACG GAGGTGCTGG AGCAGCTGAA GGCCCTACAG 
ATGGACATCA CCAGCCTGTA CAACCTCAAG TTCCAGCCGC CCACCCTGGG CCCGGAGCCT 
GCCGCCCGGA CCCCCGAGGG CAGCCCAGTA CACGGCTCCG GGCCCTCCAA GGACAGCTTT 
GGGGAGCTGA GCCGGGCCAC CATCCGGCTG CTGGAGGAAC TGGACCGGGA ACGGTGTTTC 
CTGCTGAATG AGATTGAGAA GGAGGAGAAG GAGAAGCTCT GGTACTACTC TCAGCTGCAG 
GGCCTGTCCA AGCGCCTGGA CGAGCTGCCG CACGTGGAGA CGCAGTTCTC GATGCAGATG 
GACCTGATCC GGCAGCAGCT TGAGTTCGAG GCCCAGCACA TCCGCTCGCT GATGGAGGAG 
CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCACAGA TCCGCGCCTC GCGCCTGGAG 
CAGATTGACA AGGAGCTGCT GGAGGCGCAG GACCGAGTGC AGCAGACGGA GCCCCAGGCC 
TTGCTGGCGG TGAAGTCGGT GCCGGTGGAC GAGGACCCCG AGACAGAGGT CCCCACACAC 
CCTGAGGATG GCACCCCTCA GCCGGGCAAC AGCAAGGTGG AGGTGGTCTT CTGGCTGTTG 
TCCATGTTGG CGACGCGCGA CCAGGAGGAT ACAGCGCGCA CGCTGCTGGC CATGTCCAGC 
TCGCCCGAGA GCTGCGTGGC CATGCGCCGC TCGGGCTGTC TGCCTCTGCT GCTGCAAATC 
CTCCACGGCA CCGAGGCCGC GGCCGGGGGT CGCGCCGGGG CCCCAGGGGC ACCGGGCGCC 
AAGGACGCAC GCATGCGCGC CAACGCGGCG CTGCACAACA TCGTCTTCTC GCAGCCGGAC 
CAGGGCCTGG CGCGCAAGGA GATGCGCGTC CTGCACGTGC TGGAGCAGAT CCGGGCCTAC 
TGCGAGACCT GCTGGGACTG GCTGCAGGCC CGAGACGGCG GGCCCGAGGG AGGTGGCGCC 
GGCAGCGCCC CGATCCCCAT CGAGCCGCAG ATCTGCCAGG CCACCTGTGC TGTTATGAAG 
CTGTCCTTTG ATGAGGAGTA CCGCCGTGCC ATGAACGAGC TAGGTGGGCT GCAGGCCGTG 
GCAGAGCTGC TGCAGGTTGA CTATGAGATG CACAAGATGA CCCGGGACCC GCTGAACCTG 
GCGCTGCGCC GCTACGCGGG CATGACCCTC ACCAACCTCA CCTTTGGGGA CGTTGCCAAC 
AAGGCCACCC TGTGTGCGCG CCGCGGCTGC ATGGAGGCCA TCGTGGCCCA GCTGGCCTCC 
GACAGTGAGG AGCTCCACCA GGTGGTGTCC AGCATCCTTC GGAACTTGTC CTGGAGGGCC 
GACATCAACA GCAAGAAGGT GCTGAGGGAG GCGGGCAGCG TGACTGCCCT GGTGCAGTGT 
GTCCTGCGGG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TGAGCGCCCT GTGGAATCTG 
TCTGCACACA GCACAGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGCGC CCTGGGCTTC 
CTGGTGAGCA CCCTGACCTA CAAGTGTCAG AGCAACTCGC TGGCCATCAT CGAGAGCGGC 
GGCGGCATCC TCCGCAATGT GTCCAGCCTC GTCGCCACCC GTGAGGACTA CAGGCAGGTG 
CTCCGGGATC ACAACTGTCT GCAGACGCTG CTGCAGCATC TGACTTCGCA CAGCCTGACC 
ATCGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCGC CCGTGACCAG 
GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGTA ATCTGGTGCA CTCCAAGCAC 
AAGATGATCG CCATGGGCAG CGCCGCCGCC CTGCGCAACC TGCTGGCCCA TCGGCCCGCC 
AAGCACCAGG CGGCCGCCAC CGCCGTGTCC CCAGGCAGCT GCGTGCCCAG CCTGTACGTG 
CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACGCACGGC ACCTCGCGCA GGCGCTGGAG 
CACCTGGAGA AGCAGGGCCC GCCGGCAGCC GAGGCCGCCA CTAAGAAGCC GCTGCCGCCC 
CTGCGACACC TGGACGGCCT GGCCCAAGAC TATGCTTCCG ATTCGGGCTG CTTTGACGAC 
GACGATGCAC CGTCATCCCT GGCTGCGGCC GCGGCCACCG GGGAGCCAGC CAGCCCTGCC 
GCGCTGTCCC TCTTCCTGGG CAGCCCCTTC CTGCAGGGGC AGGCGCTGGC TCGCACCCCG 
CCCACCCGCC GAGGCGGCAA GGAGGCAGAG AAGGACACCA GTGGGGAGGC AGCCGTGGCG 
GCCAAGGCCA AGGCCAAGCT GGCGCTTGCA GTGGCGCGCA TCGACCAGCT GGTGGAGGAC 
ATCTCCGCCC TGCACACCTC GTCCGACGAT AGCTTCAGCC TCAGCTCTGG AGACCCGGGA 
CAGGAGGCGC CACGGGAGGG CCGCGCCCAG TCCTGCTCGC CATGCCGCGG CCCGGAGGGC 
GGGCGGCGAG AGGCAGGAAG CCGGGCGCAC CCGCTGCTGC GGCTCAAGGC GGCCCACGCC 
AGCCTCTCCA ACGACAGCCT CAACAGCGGC AGTGCCAGCG ACGGGTACTG CCCACGCGAA 
CATATGCTGC CCTGCCCGCT GGCCGCACTG GCTTCGCGCC GCGAGGACCC CAGGTGTGGG 
CAGCCTCGGC CCAGCCGGCT TGACCTTGAC CTGCCCGGCT GCCAGGCCGA GCCCCCGGCC 
CGCGAGGCCA CCTCCGCCGA CGCCCGCGTG CGCACCATCA AGCTGTCGCC TACCTATCAG 
CACGTGCCAC TGCTTGAGGG TGCCTCAAGG GCGGGTGCAG AGCCCCTCGC GGGGCCTGGA 
ATCTCTCCAG GGGCCCGGAA GCAGGCCTGG CTGCCGGCAG ACCACCTGAG CAAGGTTCCC 
GAGAAGCTGG CGGCTGCCCC GCTGTCTGTG GCCAGCAAGG CACTGCAGAA ACTGGCGGCG 
CAAGAGGGGC CACTCTCGCT GTCCCGATGC AGCTCCCTTT CCTCGCTGTC CTCGGCCGGC 
CGCCCAGGCC CCAGCGAGGG TGGTGACCTG GATGACAGTG ACTCCTCCCT GGAGGGGCTG 
GAGGAGGCCG GCCCCAGCGA GGCTGAGCTG GACAGCACGT GGCGGGCGCC CGGGGCCACC 
TCGCTGCCCG TAGCCATTCC GGCTCCCCGG CGTAACCGAG GCCGGGGCCT GGGGGTGGAA 
GACGCCACGC CGTCCAGCTC GTCGGAGAAC TACGTGCAGG AGACACCGCT TGTGCTGAGC 
CGCTGCAGCT CTGTGAGCTC GCTGGGCAGC TTCGAGAGCC CGTCCATCGC CAGCTCCATC 
CCCAGTGAAC CTTGCAGCGG GCAGGGCAGC GGCACCATCA GCCCTAGCGA GCTGCCCGAC 
AGCCCCGGAC AGACCATGCC TCCCAGCCGG AGCAAGACGC CACCGCTGGC GCCCGCGCCA 
CAGGGTCCCC CCGAGGCCAC CCAGTTCAGC CTGCAGTGGG AGAGCTACGT GAAGCGCTTC 
CTGGACATCG CCGACTGCCG GGAGCGCTGC CGGCTGCCAT CTGAGCTGGA CGCAGGCAGC 
GTGCGCTTTA CCGTGGAGAA GCCAGACGAG AACTTCTCGT GCGCCTCCAG CCTCAGCGCG 
CTGGCCTTGC ACGAGCACTA CGTGCAGCAG GACGTGGAGC TGCGGCTGCT GCCCTCGGCC 
TGCCCCGAGC GCGGCGGGGG CGCCGGGGGC GCCGGCCTCC ACTTTGCAGG GCACCGGCGG 
CGGGAGGAGG GGCCGGCGCC CACGGGTTCT CGCCCTCGCG GCGCCGCGGA CCAGGAGCTG 
GAACTGCTGC GGGAGTGCCT GGGAGCCGCC GTGCCTGCCC GGCTGCGCAA GGTGGCCTCC 
GCGCTGGTGC CAGGTCGCCG CGCACTCCCC GTGCCCGTCT ACATGTTGGT GCCCGCCCCG 
GCCCCGGCCC AGGAGGACGA CTCCTGCACT GACTCCGCGG AGGGCACGCC GGTCAACTTC 
TCTAGCGCCG CCTCGCTCAG CGACGAGACG CTGCAGGGAC CCCCCAGGGA CCAGCCCGGG 
GGACCAGCGG GCAGGCAAAG ACCCACCGGC CGCCCCACCT CTGCCAGACA GGCCATGGGG 
CACCGGCACA AGGCGGGAGG CGCCGGCCGC AGCGCGGAGC AGTCTCGGGG CGCGGGCAAG 
AACAGAGCAG GGCTGGAGCT GCCCCTGGGC CGGCCCCCGA GCGCCCCCGC AGACAAGGAC 
GGCTCAAAGC CCGGCCGGAC CCGCGGGGAC GGGGCGCTCC AGTCGCTGTG CCTCACGACG 
CCCACTGAGG AGGCCGTGTA CTGCTTCTAC GGCAACGACT CGGACGAGGA GCCCCCGGCG 
GCCGCGCCCA CGCCAACCCA CCGGCGCACA TCGGCCATCC CTCGCGCTTT TACGCGGGAG 
CGTCCGCAGG GCCGGAAGGA GGCCCCTGCC CCGTCCAAGG CTGCACCAGC TGCCCCGCCG 
CCCGCCCGGA CCCAGCCCAG CCTCATTGCT GACGAGACCC CGCCCTGCTA CTCCCTGAGC 
TCCTCCGCCA GCTCCCTCAG CGAGCCCGAG CCCTCGGAGC CGCCGGCCGT CCATCCACGA 
GGCCGGGAGC CCGCGGTCAC CAAGGACCCG GGCCCAGGAG GCGGACGCGA CAGCTCGCCC 
AGCCCGCGGG CCGCGGAGGA GCTTCTGCAG CGGTGCATCA GCTCGGCCCT GCCCAGGCGC 
CGGCCCCCCG TGTCTGGCCT GCGGCGCCGC AAGCCCCGAG CCACCCGGCT GGATGAGCGG 
CCCGCAGAGG GGTCCCGGGA ACGCGGCGAG GAGGCAGCGG GCTCGGACCG GGCCTCCGAC 
CTGGATAGCG TGGAGTGGCG CGCCATCCAG GAGGGCGCCA ATTCAATTGT CACGTGGCTG 
CACCAGGCAG CAGCTGCCAC GCGGGAGGCC TCGTCCGAGT CCGACTCCAT CCTGTCCTTC 
GTATCCGGGC TGTCAGTGGG ATCCACCCTA CAGCCCCCCA AGCACAGGAA GGGACGACAG 
GCGGAGGGAG AAATGGGCAG TGCCCGGCGG CCAGAGAAAA GGGGCGCAGC CTCAGTCAAG 
ACCAGCGGGA GCCCCCGTTC CCCTGCAGGC CCCGAGAAGC CACGTGGCAC ACAGAAGACC 
ACGCCCGGGG TGCCAGCTGT GCTCCGGGGA CGAACAGTGA TCTACGTCCC CAGCCCGGCA 
CCCCGTGCCC AGCCCAAAGG GACCCCCGGC CCCCGCGCCA CACCGCGGAA GGTGGCGCCC 
CCTTGCCTGG CACAGCCCGC GGCTCCAGCC AAAGTCCCGA GCCCCGGGCA GCAGCGGTCG 
CGGAGCCTAC ACCGGCCTGC CAAGACCTCG GAGCTGGCGA CGCTGAGCCA GCCCCCCAGA 
AGCGCCACAC CGCCCGCCCG CCTCGCCAAG ACCCCCTCCT CCAGCTCCTC CCAGACCTCG 
CCCGCCTCCC AGCCCCTGCC CAGAAAGCGC CCCCCGGTCA CCCAGGCTGC TGGGGCCCTG 
CCCGGCCCCG GAGCCTCCCC GGTGCCCAAA ACGCCGGCGC GCACCCTTCT GGCGAAGCAG 
CACAAGACGC AGAGATCGCC CGTGCGGATC CCGTTCATGC AGAGGCCGGC CCGGCGTGGG 
CCGCCACCGC TGGCTCGGGC AGTCCCGGAG CCGGGCCCCA GGGGCCGGGC GGGGACCGAG 
GCGGGCCCGG GGGCGCGCGG GGGCCGCCTG GGCCTGGTGC GTGTGGCCTC AGCCCTCTCC 
AGCGGCAGCG AGTCCTCCGA CCGCTCGGGC TTCCGGCGAC AGCTAACCTT CATCAAGGAG 
TCGCCGGGCT TGCGGCGCCG CCGCTCCGAG CTGTCCTCGG CCGAGTCCGC GGCCTCTGCC 
CCCCAGGGCG CCTCGCCCCG CCGCGGCCGG CCCGCGCTGC CCGCCGTCTT CCTCTGCTCC 
TCGCGCTGCG AAGAGCTCCG AGCGGCACCC CGGCAGGGCC CGGCCCCGGC CCGGCAGCGG 
CCCCCCGCGG CCCGACCCAG CCCTGGCGAG CGCCCTGCCC GGCGCACCAC CTCCGAGAGC 
CCGTCCCGCC TGCCTGTGCG CGCGCCCGCC GCCCGGCCGG AGACTGTCAA GCGCTACGCG 
TCGCTGCCGC ACATCAGCGT GGCCCGCAGG CCCGACGGCG CCGTCCCCGC GGCCCCTGCC 
TCAGCCGACG CCGCGCGCCG CAGCAGCGAC GGGGAGCCCC GGCCGCTCCC CAGGGTGGCC 
GCGCCGGGCA CGACCTGGCG GCGCATCCGA GATGAGGACG TGCCCCACAT CCTGCGCAGC 
ACGCTTCCCG CCACGGCCCT GCCACTGCGG GGCTCCACGC CCGAGGACGC CCCGGCCGGG 
CCCCCGCCGC GCAAGACCAG CGACGCCGTG GTCCAGACCG AGGAGGTCGC CGCCCCCAAG 
ACCAACTCCA GCACGTCCCC GAGCCTGGAG ACCAGGGAGC CCCCCGGGGC CCCCGCCGGC 
GGCCAGCTCT CCCTCCTCGG CAGCGACGTG GACGGTCCCA GCCTCGCCAA GGCTCCCATC 
TCCGCACCCT TCGTGCACGA GGGCCTGGGG GTCGCCGTGG GGGGCTTCCC CGCCAGCCGG 
CACGGCTCCC CCAGCCGCTC GGCCCGAGTA CCCCCCTTCA ACTATGTGCC CAGCCCCATG 
GTGGTCGCAG CCACCACCGA CTCGGCCGCG GAGAAAGCCC CGGCCACTGC CTCCGCCACC 
CTCCTGGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005874.1
CDS	209..7120
Translation	MASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETQFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	NM_005883.2
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005883.2

ORF Insert Sequence:

ATGGCGAGCT CCGTGGCGCC CTACGAGCAG CTGGTGAGGC AGGTGGAGGC CTTGAAGGCT 
GAGAACAGCC ACCTGAGGCA GGAGCTAAGG GACAACTCCA GCCACCTGTC CAAGCTGGAG 
ACAGAGACGT CGGGCATGAA GGAGGTCCTG AAGCACCTAC AGGGAAAACT GGAGCAGGAG 
GCCCGAGTGC TGGTGTCCTC GGGGCAGACG GAGGTGCTGG AGCAGCTGAA GGCCCTACAG 
ATGGACATCA CCAGCCTGTA CAACCTCAAG TTCCAGCCGC CCACCCTGGG CCCGGAGCCT 
GCCGCCCGGA CCCCCGAGGG CAGCCCAGTA CACGGCTCCG GGCCCTCCAA GGACAGCTTT 
GGGGAGCTGA GCCGGGCCAC CATCCGGCTG CTGGAGGAAC TGGACCGGGA ACGGTGTTTC 
CTGCTGAATG AGATTGAGAA GGAGGAGAAG GAGAAGCTCT GGTACTACTC TCAGCTGCAG 
GGCCTGTCCA AGCGCCTGGA CGAGCTGCCG CACGTGGAGA CGCAGTTCTC GATGCAGATG 
GACCTGATCC GGCAGCAGCT TGAGTTCGAG GCCCAGCACA TCCGCTCGCT GATGGAGGAG 
CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCACAGA TCCGCGCCTC GCGCCTGGAG 
CAGATTGACA AGGAGCTGCT GGAGGCGCAG GACCGAGTGC AGCAGACGGA GCCCCAGGCC 
TTGCTGGCGG TGAAGTCGGT GCCGGTGGAC GAGGACCCCG AGACAGAGGT CCCCACACAC 
CCTGAGGATG GCACCCCTCA GCCGGGCAAC AGCAAGGTGG AGGTGGTCTT CTGGCTGTTG 
TCCATGTTGG CGACGCGCGA CCAGGAGGAT ACAGCGCGCA CGCTGCTGGC CATGTCCAGC 
TCGCCCGAGA GCTGCGTGGC CATGCGCCGC TCGGGCTGTC TGCCTCTGCT GCTGCAAATC 
CTCCACGGCA CCGAGGCCGC GGCCGGGGGT CGCGCCGGGG CCCCAGGGGC ACCGGGCGCC 
AAGGACGCAC GCATGCGCGC CAACGCGGCG CTGCACAACA TCGTCTTCTC GCAGCCGGAC 
CAGGGCCTGG CGCGCAAGGA GATGCGCGTC CTGCACGTGC TGGAGCAGAT CCGGGCCTAC 
TGCGAGACCT GCTGGGACTG GCTGCAGGCC CGAGACGGCG GGCCCGAGGG AGGTGGCGCC 
GGCAGCGCCC CGATCCCCAT CGAGCCGCAG ATCTGCCAGG CCACCTGTGC TGTTATGAAG 
CTGTCCTTTG ATGAGGAGTA CCGCCGTGCC ATGAACGAGC TAGGTGGGCT GCAGGCCGTG 
GCAGAGCTGC TGCAGGTTGA CTATGAGATG CACAAGATGA CCCGGGACCC GCTGAACCTG 
GCGCTGCGCC GCTACGCGGG CATGACCCTC ACCAACCTCA CCTTTGGGGA CGTTGCCAAC 
AAGGCCACCC TGTGTGCGCG CCGCGGCTGC ATGGAGGCCA TCGTGGCCCA GCTGGCCTCC 
GACAGTGAGG AGCTCCACCA GGTGGTGTCC AGCATCCTTC GGAACTTGTC CTGGAGGGCC 
GACATCAACA GCAAGAAGGT GCTGAGGGAG GCGGGCAGCG TGACTGCCCT GGTGCAGTGT 
GTCCTGCGGG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TGAGCGCCCT GTGGAATCTG 
TCTGCACACA GCACAGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGCGC CCTGGGCTTC 
CTGGTGAGCA CCCTGACCTA CAAGTGTCAG AGCAACTCGC TGGCCATCAT CGAGAGCGGC 
GGCGGCATCC TCCGCAATGT GTCCAGCCTC GTCGCCACCC GTGAGGACTA CAGGCAGGTG 
CTCCGGGATC ACAACTGTCT GCAGACGCTG CTGCAGCATC TGACTTCGCA CAGCCTGACC 
ATCGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCGC CCGTGACCAG 
GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGTA ATCTGGTGCA CTCCAAGCAC 
AAGATGATCG CCATGGGCAG CGCCGCCGCC CTGCGCAACC TGCTGGCCCA TCGGCCCGCC 
AAGCACCAGG CGGCCGCCAC CGCCGTGTCC CCAGGCAGCT GCGTGCCCAG CCTGTACGTG 
CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACGCACGGC ACCTCGCGCA GGCGCTGGAG 
CACCTGGAGA AGCAGGGCCC GCCGGCAGCC GAGGCCGCCA CTAAGAAGCC GCTGCCGCCC 
CTGCGACACC TGGACGGCCT GGCCCAAGAC TATGCTTCCG ATTCGGGCTG CTTTGACGAC 
GACGATGCAC CGTCATCCCT GGCTGCGGCC GCGGCCACCG GGGAGCCAGC CAGCCCTGCC 
GCGCTGTCCC TCTTCCTGGG CAGCCCCTTC CTGCAGGGGC AGGCGCTGGC TCGCACCCCG 
CCCACCCGCC GAGGCGGCAA GGAGGCAGAG AAGGACACCA GTGGGGAGGC AGCCGTGGCG 
GCCAAGGCCA AGGCCAAGCT GGCGCTTGCA GTGGCGCGCA TCGACCAGCT GGTGGAGGAC 
ATCTCCGCCC TGCACACCTC GTCCGACGAT AGCTTCAGCC TCAGCTCTGG AGACCCGGGA 
CAGGAGGCGC CACGGGAGGG CCGCGCCCAG TCCTGCTCGC CATGCCGCGG CCCGGAGGGC 
GGGCGGCGAG AGGCAGGAAG CCGGGCGCAC CCGCTGCTGC GGCTCAAGGC GGCCCACGCC 
AGCCTCTCCA ACGACAGCCT CAACAGCGGC AGTGCCAGCG ACGGGTACTG CCCACGCGAA 
CATATGCTGC CCTGCCCGCT GGCCGCACTG GCTTCGCGCC GCGAGGACCC CAGGTGTGGG 
CAGCCTCGGC CCAGCCGGCT TGACCTTGAC CTGCCCGGCT GCCAGGCCGA GCCCCCGGCC 
CGCGAGGCCA CCTCCGCCGA CGCCCGCGTG CGCACCATCA AGCTGTCGCC TACCTATCAG 
CACGTGCCAC TGCTTGAGGG TGCCTCAAGG GCGGGTGCAG AGCCCCTCGC GGGGCCTGGA 
ATCTCTCCAG GGGCCCGGAA GCAGGCCTGG CTGCCGGCAG ACCACCTGAG CAAGGTTCCC 
GAGAAGCTGG CGGCTGCCCC GCTGTCTGTG GCCAGCAAGG CACTGCAGAA ACTGGCGGCG 
CAAGAGGGGC CACTCTCGCT GTCCCGATGC AGCTCCCTTT CCTCGCTGTC CTCGGCCGGC 
CGCCCAGGCC CCAGCGAGGG TGGTGACCTG GATGACAGTG ACTCCTCCCT GGAGGGGCTG 
GAGGAGGCCG GCCCCAGCGA GGCTGAGCTG GACAGCACGT GGCGGGCGCC CGGGGCCACC 
TCGCTGCCCG TAGCCATTCC GGCTCCCCGG CGTAACCGAG GCCGGGGCCT GGGGGTGGAA 
GACGCCACGC CGTCCAGCTC GTCGGAGAAC TACGTGCAGG AGACACCGCT TGTGCTGAGC 
CGCTGCAGCT CTGTGAGCTC GCTGGGCAGC TTCGAGAGCC CGTCCATCGC CAGCTCCATC 
CCCAGTGAAC CTTGCAGCGG GCAGGGCAGC GGCACCATCA GCCCTAGCGA GCTGCCCGAC 
AGCCCCGGAC AGACCATGCC TCCCAGCCGG AGCAAGACGC CACCGCTGGC GCCCGCGCCA 
CAGGGTCCCC CCGAGGCCAC CCAGTTCAGC CTGCAGTGGG AGAGCTACGT GAAGCGCTTC 
CTGGACATCG CCGACTGCCG GGAGCGCTGC CGGCTGCCAT CTGAGCTGGA CGCAGGCAGC 
GTGCGCTTTA CCGTGGAGAA GCCAGACGAG AACTTCTCGT GCGCCTCCAG CCTCAGCGCG 
CTGGCCTTGC ACGAGCACTA CGTGCAGCAG GACGTGGAGC TGCGGCTGCT GCCCTCGGCC 
TGCCCCGAGC GCGGCGGGGG CGCCGGGGGC GCCGGCCTCC ACTTTGCAGG GCACCGGCGG 
CGGGAGGAGG GGCCGGCGCC CACGGGTTCT CGCCCTCGCG GCGCCGCGGA CCAGGAGCTG 
GAACTGCTGC GGGAGTGCCT GGGAGCCGCC GTGCCTGCCC GGCTGCGCAA GGTGGCCTCC 
GCGCTGGTGC CAGGTCGCCG CGCACTCCCC GTGCCCGTCT ACATGTTGGT GCCCGCCCCG 
GCCCCGGCCC AGGAGGACGA CTCCTGCACT GACTCCGCGG AGGGCACGCC GGTCAACTTC 
TCTAGCGCCG CCTCGCTCAG CGACGAGACG CTGCAGGGAC CCCCCAGGGA CCAGCCCGGG 
GGACCAGCGG GCAGGCAAAG ACCCACCGGC CGCCCCACCT CTGCCAGACA GGCCATGGGG 
CACCGGCACA AGGCGGGAGG CGCCGGCCGC AGCGCGGAGC AGTCTCGGGG CGCGGGCAAG 
AACAGAGCAG GGCTGGAGCT GCCCCTGGGC CGGCCCCCGA GCGCCCCCGC AGACAAGGAC 
GGCTCAAAGC CCGGCCGGAC CCGCGGGGAC GGGGCGCTCC AGTCGCTGTG CCTCACGACG 
CCCACTGAGG AGGCCGTGTA CTGCTTCTAC GGCAACGACT CGGACGAGGA GCCCCCGGCG 
GCCGCGCCCA CGCCAACCCA CCGGCGCACA TCGGCCATCC CTCGCGCTTT TACGCGGGAG 
CGTCCGCAGG GCCGGAAGGA GGCCCCTGCC CCGTCCAAGG CTGCACCAGC TGCCCCGCCG 
CCCGCCCGGA CCCAGCCCAG CCTCATTGCT GACGAGACCC CGCCCTGCTA CTCCCTGAGC 
TCCTCCGCCA GCTCCCTCAG CGAGCCCGAG CCCTCGGAGC CGCCGGCCGT CCATCCACGA 
GGCCGGGAGC CCGCGGTCAC CAAGGACCCG GGCCCAGGAG GCGGACGCGA CAGCTCGCCC 
AGCCCGCGGG CCGCGGAGGA GCTTCTGCAG CGGTGCATCA GCTCGGCCCT GCCCAGGCGC 
CGGCCCCCCG TGTCTGGCCT GCGGCGCCGC AAGCCCCGAG CCACCCGGCT GGATGAGCGG 
CCCGCAGAGG GGTCCCGGGA ACGCGGCGAG GAGGCAGCGG GCTCGGACCG GGCCTCCGAC 
CTGGATAGCG TGGAGTGGCG CGCCATCCAG GAGGGCGCCA ATTCAATTGT CACGTGGCTG 
CACCAGGCAG CAGCTGCCAC GCGGGAGGCC TCGTCCGAGT CCGACTCCAT CCTGTCCTTC 
GTATCCGGGC TGTCAGTGGG ATCCACCCTA CAGCCCCCCA AGCACAGGAA GGGACGACAG 
GCGGAGGGAG AAATGGGCAG TGCCCGGCGG CCAGAGAAAA GGGGCGCAGC CTCAGTCAAG 
ACCAGCGGGA GCCCCCGTTC CCCTGCAGGC CCCGAGAAGC CACGTGGCAC ACAGAAGACC 
ACGCCCGGGG TGCCAGCTGT GCTCCGGGGA CGAACAGTGA TCTACGTCCC CAGCCCGGCA 
CCCCGTGCCC AGCCCAAAGG GACCCCCGGC CCCCGCGCCA CACCGCGGAA GGTGGCGCCC 
CCTTGCCTGG CACAGCCCGC GGCTCCAGCC AAAGTCCCGA GCCCCGGGCA GCAGCGGTCG 
CGGAGCCTAC ACCGGCCTGC CAAGACCTCG GAGCTGGCGA CGCTGAGCCA GCCCCCCAGA 
AGCGCCACAC CGCCCGCCCG CCTCGCCAAG ACCCCCTCCT CCAGCTCCTC CCAGACCTCG 
CCCGCCTCCC AGCCCCTGCC CAGAAAGCGC CCCCCGGTCA CCCAGGCTGC TGGGGCCCTG 
CCCGGCCCCG GAGCCTCCCC GGTGCCCAAA ACGCCGGCGC GCACCCTTCT GGCGAAGCAG 
CACAAGACGC AGAGATCGCC CGTGCGGATC CCGTTCATGC AGAGGCCGGC CCGGCGTGGG 
CCGCCACCGC TGGCTCGGGC AGTCCCGGAG CCGGGCCCCA GGGGCCGGGC GGGGACCGAG 
GCGGGCCCGG GGGCGCGCGG GGGCCGCCTG GGCCTGGTGC GTGTGGCCTC AGCCCTCTCC 
AGCGGCAGCG AGTCCTCCGA CCGCTCGGGC TTCCGGCGAC AGCTAACCTT CATCAAGGAG 
TCGCCGGGCT TGCGGCGCCG CCGCTCCGAG CTGTCCTCGG CCGAGTCCGC GGCCTCTGCC 
CCCCAGGGCG CCTCGCCCCG CCGCGGCCGG CCCGCGCTGC CCGCCGTCTT CCTCTGCTCC 
TCGCGCTGCG AAGAGCTCCG AGCGGCACCC CGGCAGGGCC CGGCCCCGGC CCGGCAGCGG 
CCCCCCGCGG CCCGACCCAG CCCTGGCGAG CGCCCTGCCC GGCGCACCAC CTCCGAGAGC 
CCGTCCCGCC TGCCTGTGCG CGCGCCCGCC GCCCGGCCGG AGACTGTCAA GCGCTACGCG 
TCGCTGCCGC ACATCAGCGT GGCCCGCAGG CCCGACGGCG CCGTCCCCGC GGCCCCTGCC 
TCAGCCGACG CCGCGCGCCG CAGCAGCGAC GGGGAGCCCC GGCCGCTCCC CAGGGTGGCC 
GCGCCGGGCA CGACCTGGCG GCGCATCCGA GATGAGGACG TGCCCCACAT CCTGCGCAGC 
ACGCTTCCCG CCACGGCCCT GCCACTGCGG GGCTCCACGC CCGAGGACGC CCCGGCCGGG 
CCCCCGCCGC GCAAGACCAG CGACGCCGTG GTCCAGACCG AGGAGGTCGC CGCCCCCAAG 
ACCAACTCCA GCACGTCCCC GAGCCTGGAG ACCAGGGAGC CCCCCGGGGC CCCCGCCGGC 
GGCCAGCTCT CCCTCCTCGG CAGCGACGTG GACGGTCCCA GCCTCGCCAA GGCTCCCATC 
TCCGCACCCT TCGTGCACGA GGGCCTGGGG GTCGCCGTGG GGGGCTTCCC CGCCAGCCGG 
CACGGCTCCC CCAGCCGCTC GGCCCGAGTA CCCCCCTTCA ACTATGTGCC CAGCCCCATG 
GTGGTCGCAG CCACCACCGA CTCGGCCGCG GAGAAAGCCC CGGCCACTGC CTCCGCCACC 
CTCCTGGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APC2 ( NM_001351273.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338202.1 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant 2, mRNA.

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CloneID	OHu51394
Clone ID Related Accession (Same CDS sequence)	NM_001351273.1
Accession Version	NM_001351273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027307.5. Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the 5' coding region, compared to variant 1. This difference results in a protein (isoform 2) that is one amino acid shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF128222.1, AJ012652.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGAGCT CCGTGGCGCC CTACGAGCAG CTGGTGAGGC AGGTGGAGGC CTTGAAGGCT 
GAGAACAGCC ACCTGAGGCA GGAGCTAAGG GACAACTCCA GCCACCTGTC CAAGCTGGAG 
ACAGAGACGT CGGGCATGAA GGAGGTCCTG AAGCACCTAC AGGGAAAACT GGAGCAGGAG 
GCCCGAGTGC TGGTGTCCTC GGGGCAGACG GAGGTGCTGG AGCAGCTGAA GGCCCTACAG 
ATGGACATCA CCAGCCTGTA CAACCTCAAG TTCCAGCCGC CCACCCTGGG CCCGGAGCCT 
GCCGCCCGGA CCCCCGAGGG CAGCCCAGTA CACGGCTCCG GGCCCTCCAA GGACAGCTTT 
GGGGAGCTGA GCCGGGCCAC CATCCGGCTG CTGGAGGAAC TGGACCGGGA ACGGTGTTTC 
CTGCTGAATG AGATTGAGAA GGAGGAGAAG GAGAAGCTCT GGTACTACTC TCAGCTGCAG 
GGCCTGTCCA AGCGCCTGGA CGAGCTGCCG CACGTGGAGA CGTTCTCGAT GCAGATGGAC 
CTGATCCGGC AGCAGCTTGA GTTCGAGGCC CAGCACATCC GCTCGCTGAT GGAGGAGCGC 
TTCGGCACCT CGGACGAGAT GGTGCAGCGG GCACAGATCC GCGCCTCGCG CCTGGAGCAG 
ATTGACAAGG AGCTGCTGGA GGCGCAGGAC CGAGTGCAGC AGACGGAGCC CCAGGCCTTG 
CTGGCGGTGA AGTCGGTGCC GGTGGACGAG GACCCCGAGA CAGAGGTCCC CACACACCCT 
GAGGATGGCA CCCCTCAGCC GGGCAACAGC AAGGTGGAGG TGGTCTTCTG GCTGTTGTCC 
ATGTTGGCGA CGCGCGACCA GGAGGATACA GCGCGCACGC TGCTGGCCAT GTCCAGCTCG 
CCCGAGAGCT GCGTGGCCAT GCGCCGCTCG GGCTGTCTGC CTCTGCTGCT GCAAATCCTC 
CACGGCACCG AGGCCGCGGC CGGGGGTCGC GCCGGGGCCC CAGGGGCACC GGGCGCCAAG 
GACGCACGCA TGCGCGCCAA CGCGGCGCTG CACAACATCG TCTTCTCGCA GCCGGACCAG 
GGCCTGGCGC GCAAGGAGAT GCGCGTCCTG CACGTGCTGG AGCAGATCCG GGCCTACTGC 
GAGACCTGCT GGGACTGGCT GCAGGCCCGA GACGGCGGGC CCGAGGGAGG TGGCGCCGGC 
AGCGCCCCGA TCCCCATCGA GCCGCAGATC TGCCAGGCCA CCTGTGCTGT TATGAAGCTG 
TCCTTTGATG AGGAGTACCG CCGTGCCATG AACGAGCTAG GTGGGCTGCA GGCCGTGGCA 
GAGCTGCTGC AGGTTGACTA TGAGATGCAC AAGATGACCC GGGACCCGCT GAACCTGGCG 
CTGCGCCGCT ACGCGGGCAT GACCCTCACC AACCTCACCT TTGGGGACGT TGCCAACAAG 
GCCACCCTGT GTGCGCGCCG CGGCTGCATG GAGGCCATCG TGGCCCAGCT GGCCTCCGAC 
AGTGAGGAGC TCCACCAGGT GGTGTCCAGC ATCCTTCGGA ACTTGTCCTG GAGGGCCGAC 
ATCAACAGCA AGAAGGTGCT GAGGGAGGCG GGCAGCGTGA CTGCCCTGGT GCAGTGTGTC 
CTGCGGGCCA CCAAGGAGTC CACCCTGAAG AGCGTGCTGA GCGCCCTGTG GAATCTGTCT 
GCACACAGCA CAGAGAACAA GGCGGCCATC TGCCAGGTGG ATGGCGCCCT GGGCTTCCTG 
GTGAGCACCC TGACCTACAA GTGTCAGAGC AACTCGCTGG CCATCATCGA GAGCGGCGGC 
GGCATCCTCC GCAATGTGTC CAGCCTCGTC GCCACCCGTG AGGACTACAG GCAGGTGCTC 
CGGGATCACA ACTGTCTGCA GACGCTGCTG CAGCATCTGA CTTCGCACAG CCTGACCATC 
GTGAGCAACG CGTGCGGCAC GCTCTGGAAC CTGTCGGCCC GCAGCGCCCG TGACCAGGAG 
CTGCTGTGGG ACCTGGGCGC CGTGGGCATG CTGCGTAATC TGGTGCACTC CAAGCACAAG 
ATGATCGCCA TGGGCAGCGC CGCCGCCCTG CGCAACCTGC TGGCCCATCG GCCCGCCAAG 
CACCAGGCGG CCGCCACCGC CGTGTCCCCA GGCAGCTGCG TGCCCAGCCT GTACGTGCGC 
AAGCAGCGGG CGCTGGAGGC CGAGCTGGAC GCACGGCACC TCGCGCAGGC GCTGGAGCAC 
CTGGAGAAGC AGGGCCCGCC GGCAGCCGAG GCCGCCACTA AGAAGCCGCT GCCGCCCCTG 
CGACACCTGG ACGGCCTGGC CCAAGACTAT GCTTCCGATT CGGGCTGCTT TGACGACGAC 
GATGCACCGT CATCCCTGGC TGCGGCCGCG GCCACCGGGG AGCCAGCCAG CCCTGCCGCG 
CTGTCCCTCT TCCTGGGCAG CCCCTTCCTG CAGGGGCAGG CGCTGGCTCG CACCCCGCCC 
ACCCGCCGAG GCGGCAAGGA GGCAGAGAAG GACACCAGTG GGGAGGCAGC CGTGGCGGCC 
AAGGCCAAGG CCAAGCTGGC GCTTGCAGTG GCGCGCATCG ACCAGCTGGT GGAGGACATC 
TCCGCCCTGC ACACCTCGTC CGACGATAGC TTCAGCCTCA GCTCTGGAGA CCCGGGACAG 
GAGGCGCCAC GGGAGGGCCG CGCCCAGTCC TGCTCGCCAT GCCGCGGCCC GGAGGGCGGG 
CGGCGAGAGG CAGGAAGCCG GGCGCACCCG CTGCTGCGGC TCAAGGCGGC CCACGCCAGC 
CTCTCCAACG ACAGCCTCAA CAGCGGCAGT GCCAGCGACG GGTACTGCCC ACGCGAACAT 
ATGCTGCCCT GCCCGCTGGC CGCACTGGCT TCGCGCCGCG AGGACCCCAG GTGTGGGCAG 
CCTCGGCCCA GCCGGCTTGA CCTTGACCTG CCCGGCTGCC AGGCCGAGCC CCCGGCCCGC 
GAGGCCACCT CCGCCGACGC CCGCGTGCGC ACCATCAAGC TGTCGCCTAC CTATCAGCAC 
GTGCCACTGC TTGAGGGTGC CTCAAGGGCG GGTGCAGAGC CCCTCGCGGG GCCTGGAATC 
TCTCCAGGGG CCCGGAAGCA GGCCTGGCTG CCGGCAGACC ACCTGAGCAA GGTTCCCGAG 
AAGCTGGCGG CTGCCCCGCT GTCTGTGGCC AGCAAGGCAC TGCAGAAACT GGCGGCGCAA 
GAGGGGCCAC TCTCGCTGTC CCGATGCAGC TCCCTTTCCT CGCTGTCCTC GGCCGGCCGC 
CCAGGCCCCA GCGAGGGTGG TGACCTGGAT GACAGTGACT CCTCCCTGGA GGGGCTGGAG 
GAGGCCGGCC CCAGCGAGGC TGAGCTGGAC AGCACGTGGC GGGCGCCCGG GGCCACCTCG 
CTGCCCGTAG CCATTCCGGC TCCCCGGCGT AACCGAGGCC GGGGCCTGGG GGTGGAAGAC 
GCCACGCCGT CCAGCTCGTC GGAGAACTAC GTGCAGGAGA CACCGCTTGT GCTGAGCCGC 
TGCAGCTCTG TGAGCTCGCT GGGCAGCTTC GAGAGCCCGT CCATCGCCAG CTCCATCCCC 
AGTGAACCTT GCAGCGGGCA GGGCAGCGGC ACCATCAGCC CTAGCGAGCT GCCCGACAGC 
CCCGGACAGA CCATGCCTCC CAGCCGGAGC AAGACGCCAC CGCTGGCGCC CGCGCCACAG 
GGTCCCCCCG AGGCCACCCA GTTCAGCCTG CAGTGGGAGA GCTACGTGAA GCGCTTCCTG 
GACATCGCCG ACTGCCGGGA GCGCTGCCGG CTGCCATCTG AGCTGGACGC AGGCAGCGTG 
CGCTTTACCG TGGAGAAGCC AGACGAGAAC TTCTCGTGCG CCTCCAGCCT CAGCGCGCTG 
GCCTTGCACG AGCACTACGT GCAGCAGGAC GTGGAGCTGC GGCTGCTGCC CTCGGCCTGC 
CCCGAGCGCG GCGGGGGCGC CGGGGGCGCC GGCCTCCACT TTGCAGGGCA CCGGCGGCGG 
GAGGAGGGGC CGGCGCCCAC GGGTTCTCGC CCTCGCGGCG CCGCGGACCA GGAGCTGGAA 
CTGCTGCGGG AGTGCCTGGG AGCCGCCGTG CCTGCCCGGC TGCGCAAGGT GGCCTCCGCG 
CTGGTGCCAG GTCGCCGCGC ACTCCCCGTG CCCGTCTACA TGTTGGTGCC CGCCCCGGCC 
CCGGCCCAGG AGGACGACTC CTGCACTGAC TCCGCGGAGG GCACGCCGGT CAACTTCTCT 
AGCGCCGCCT CGCTCAGCGA CGAGACGCTG CAGGGACCCC CCAGGGACCA GCCCGGGGGA 
CCAGCGGGCA GGCAAAGACC CACCGGCCGC CCCACCTCTG CCAGACAGGC CATGGGGCAC 
CGGCACAAGG CGGGAGGCGC CGGCCGCAGC GCGGAGCAGT CTCGGGGCGC GGGCAAGAAC 
AGAGCAGGGC TGGAGCTGCC CCTGGGCCGG CCCCCGAGCG CCCCCGCAGA CAAGGACGGC 
TCAAAGCCCG GCCGGACCCG CGGGGACGGG GCGCTCCAGT CGCTGTGCCT CACGACGCCC 
ACTGAGGAGG CCGTGTACTG CTTCTACGGC AACGACTCGG ACGAGGAGCC CCCGGCGGCC 
GCGCCCACGC CAACCCACCG GCGCACATCG GCCATCCCTC GCGCTTTTAC GCGGGAGCGT 
CCGCAGGGCC GGAAGGAGGC CCCTGCCCCG TCCAAGGCTG CACCAGCTGC CCCGCCGCCC 
GCCCGGACCC AGCCCAGCCT CATTGCTGAC GAGACCCCGC CCTGCTACTC CCTGAGCTCC 
TCCGCCAGCT CCCTCAGCGA GCCCGAGCCC TCGGAGCCGC CGGCCGTCCA TCCACGAGGC 
CGGGAGCCCG CGGTCACCAA GGACCCGGGC CCAGGAGGCG GACGCGACAG CTCGCCCAGC 
CCGCGGGCCG CGGAGGAGCT TCTGCAGCGG TGCATCAGCT CGGCCCTGCC CAGGCGCCGG 
CCCCCCGTGT CTGGCCTGCG GCGCCGCAAG CCCCGAGCCA CCCGGCTGGA TGAGCGGCCC 
GCAGAGGGGT CCCGGGAACG CGGCGAGGAG GCAGCGGGCT CGGACCGGGC CTCCGACCTG 
GATAGCGTGG AGTGGCGCGC CATCCAGGAG GGCGCCAATT CAATTGTCAC GTGGCTGCAC 
CAGGCAGCAG CTGCCACGCG GGAGGCCTCG TCCGAGTCCG ACTCCATCCT GTCCTTCGTA 
TCCGGGCTGT CAGTGGGATC CACCCTACAG CCCCCCAAGC ACAGGAAGGG ACGACAGGCG 
GAGGGAGAAA TGGGCAGTGC CCGGCGGCCA GAGAAAAGGG GCGCAGCCTC AGTCAAGACC 
AGCGGGAGCC CCCGTTCCCC TGCAGGCCCC GAGAAGCCAC GTGGCACACA GAAGACCACG 
CCCGGGGTGC CAGCTGTGCT CCGGGGACGA ACAGTGATCT ACGTCCCCAG CCCGGCACCC 
CGTGCCCAGC CCAAAGGGAC CCCCGGCCCC CGCGCCACAC CGCGGAAGGT GGCGCCCCCT 
TGCCTGGCAC AGCCCGCGGC TCCAGCCAAA GTCCCGAGCC CCGGGCAGCA GCGGTCGCGG 
AGCCTACACC GGCCTGCCAA GACCTCGGAG CTGGCGACGC TGAGCCAGCC CCCCAGAAGC 
GCCACACCGC CCGCCCGCCT CGCCAAGACC CCCTCCTCCA GCTCCTCCCA GACCTCGCCC 
GCCTCCCAGC CCCTGCCCAG AAAGCGCCCC CCGGTCACCC AGGCTGCTGG GGCCCTGCCC 
GGCCCCGGAG CCTCCCCGGT GCCCAAAACG CCGGCGCGCA CCCTTCTGGC GAAGCAGCAC 
AAGACGCAGA GATCGCCCGT GCGGATCCCG TTCATGCAGA GGCCGGCCCG GCGTGGGCCG 
CCACCGCTGG CTCGGGCAGT CCCGGAGCCG GGCCCCAGGG GCCGGGCGGG GACCGAGGCG 
GGCCCGGGGG CGCGCGGGGG CCGCCTGGGC CTGGTGCGTG TGGCCTCAGC CCTCTCCAGC 
GGCAGCGAGT CCTCCGACCG CTCGGGCTTC CGGCGACAGC TAACCTTCAT CAAGGAGTCG 
CCGGGCTTGC GGCGCCGCCG CTCCGAGCTG TCCTCGGCCG AGTCCGCGGC CTCTGCCCCC 
CAGGGCGCCT CGCCCCGCCG CGGCCGGCCC GCGCTGCCCG CCGTCTTCCT CTGCTCCTCG 
CGCTGCGAAG AGCTCCGAGC GGCACCCCGG CAGGGCCCGG CCCCGGCCCG GCAGCGGCCC 
CCCGCGGCCC GACCCAGCCC TGGCGAGCGC CCTGCCCGGC GCACCACCTC CGAGAGCCCG 
TCCCGCCTGC CTGTGCGCGC GCCCGCCGCC CGGCCGGAGA CTGTCAAGCG CTACGCGTCG 
CTGCCGCACA TCAGCGTGGC CCGCAGGCCC GACGGCGCCG TCCCCGCGGC CCCTGCCTCA 
GCCGACGCCG CGCGCCGCAG CAGCGACGGG GAGCCCCGGC CGCTCCCCAG GGTGGCCGCG 
CCGGGCACGA CCTGGCGGCG CATCCGAGAT GAGGACGTGC CCCACATCCT GCGCAGCACG 
CTTCCCGCCA CGGCCCTGCC ACTGCGGGGC TCCACGCCCG AGGACGCCCC GGCCGGGCCC 
CCGCCGCGCA AGACCAGCGA CGCCGTGGTC CAGACCGAGG AGGTCGCCGC CCCCAAGACC 
AACTCCAGCA CGTCCCCGAG CCTGGAGACC AGGGAGCCCC CCGGGGCCCC CGCCGGCGGC 
CAGCTCTCCC TCCTCGGCAG CGACGTGGAC GGTCCCAGCC TCGCCAAGGC TCCCATCTCC 
GCACCCTTCG TGCACGAGGG CCTGGGGGTC GCCGTGGGGG GCTTCCCCGC CAGCCGGCAC 
GGCTCCCCCA GCCGCTCGGC CCGAGTACCC CCCTTCAACT ATGTGCCCAG CCCCATGGTG 
GTCGCAGCCA CCACCGACTC GGCCGCGGAG AAAGCCCCGG CCACTGCCTC CGCCACCCTC 
CTGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338202.1
CDS	19..6927
Translation	MASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	NM_001351273.1
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351273.1

ORF Insert Sequence:

ATGGCGAGCT CCGTGGCGCC CTACGAGCAG CTGGTGAGGC AGGTGGAGGC CTTGAAGGCT 
GAGAACAGCC ACCTGAGGCA GGAGCTAAGG GACAACTCCA GCCACCTGTC CAAGCTGGAG 
ACAGAGACGT CGGGCATGAA GGAGGTCCTG AAGCACCTAC AGGGAAAACT GGAGCAGGAG 
GCCCGAGTGC TGGTGTCCTC GGGGCAGACG GAGGTGCTGG AGCAGCTGAA GGCCCTACAG 
ATGGACATCA CCAGCCTGTA CAACCTCAAG TTCCAGCCGC CCACCCTGGG CCCGGAGCCT 
GCCGCCCGGA CCCCCGAGGG CAGCCCAGTA CACGGCTCCG GGCCCTCCAA GGACAGCTTT 
GGGGAGCTGA GCCGGGCCAC CATCCGGCTG CTGGAGGAAC TGGACCGGGA ACGGTGTTTC 
CTGCTGAATG AGATTGAGAA GGAGGAGAAG GAGAAGCTCT GGTACTACTC TCAGCTGCAG 
GGCCTGTCCA AGCGCCTGGA CGAGCTGCCG CACGTGGAGA CGTTCTCGAT GCAGATGGAC 
CTGATCCGGC AGCAGCTTGA GTTCGAGGCC CAGCACATCC GCTCGCTGAT GGAGGAGCGC 
TTCGGCACCT CGGACGAGAT GGTGCAGCGG GCACAGATCC GCGCCTCGCG CCTGGAGCAG 
ATTGACAAGG AGCTGCTGGA GGCGCAGGAC CGAGTGCAGC AGACGGAGCC CCAGGCCTTG 
CTGGCGGTGA AGTCGGTGCC GGTGGACGAG GACCCCGAGA CAGAGGTCCC CACACACCCT 
GAGGATGGCA CCCCTCAGCC GGGCAACAGC AAGGTGGAGG TGGTCTTCTG GCTGTTGTCC 
ATGTTGGCGA CGCGCGACCA GGAGGATACA GCGCGCACGC TGCTGGCCAT GTCCAGCTCG 
CCCGAGAGCT GCGTGGCCAT GCGCCGCTCG GGCTGTCTGC CTCTGCTGCT GCAAATCCTC 
CACGGCACCG AGGCCGCGGC CGGGGGTCGC GCCGGGGCCC CAGGGGCACC GGGCGCCAAG 
GACGCACGCA TGCGCGCCAA CGCGGCGCTG CACAACATCG TCTTCTCGCA GCCGGACCAG 
GGCCTGGCGC GCAAGGAGAT GCGCGTCCTG CACGTGCTGG AGCAGATCCG GGCCTACTGC 
GAGACCTGCT GGGACTGGCT GCAGGCCCGA GACGGCGGGC CCGAGGGAGG TGGCGCCGGC 
AGCGCCCCGA TCCCCATCGA GCCGCAGATC TGCCAGGCCA CCTGTGCTGT TATGAAGCTG 
TCCTTTGATG AGGAGTACCG CCGTGCCATG AACGAGCTAG GTGGGCTGCA GGCCGTGGCA 
GAGCTGCTGC AGGTTGACTA TGAGATGCAC AAGATGACCC GGGACCCGCT GAACCTGGCG 
CTGCGCCGCT ACGCGGGCAT GACCCTCACC AACCTCACCT TTGGGGACGT TGCCAACAAG 
GCCACCCTGT GTGCGCGCCG CGGCTGCATG GAGGCCATCG TGGCCCAGCT GGCCTCCGAC 
AGTGAGGAGC TCCACCAGGT GGTGTCCAGC ATCCTTCGGA ACTTGTCCTG GAGGGCCGAC 
ATCAACAGCA AGAAGGTGCT GAGGGAGGCG GGCAGCGTGA CTGCCCTGGT GCAGTGTGTC 
CTGCGGGCCA CCAAGGAGTC CACCCTGAAG AGCGTGCTGA GCGCCCTGTG GAATCTGTCT 
GCACACAGCA CAGAGAACAA GGCGGCCATC TGCCAGGTGG ATGGCGCCCT GGGCTTCCTG 
GTGAGCACCC TGACCTACAA GTGTCAGAGC AACTCGCTGG CCATCATCGA GAGCGGCGGC 
GGCATCCTCC GCAATGTGTC CAGCCTCGTC GCCACCCGTG AGGACTACAG GCAGGTGCTC 
CGGGATCACA ACTGTCTGCA GACGCTGCTG CAGCATCTGA CTTCGCACAG CCTGACCATC 
GTGAGCAACG CGTGCGGCAC GCTCTGGAAC CTGTCGGCCC GCAGCGCCCG TGACCAGGAG 
CTGCTGTGGG ACCTGGGCGC CGTGGGCATG CTGCGTAATC TGGTGCACTC CAAGCACAAG 
ATGATCGCCA TGGGCAGCGC CGCCGCCCTG CGCAACCTGC TGGCCCATCG GCCCGCCAAG 
CACCAGGCGG CCGCCACCGC CGTGTCCCCA GGCAGCTGCG TGCCCAGCCT GTACGTGCGC 
AAGCAGCGGG CGCTGGAGGC CGAGCTGGAC GCACGGCACC TCGCGCAGGC GCTGGAGCAC 
CTGGAGAAGC AGGGCCCGCC GGCAGCCGAG GCCGCCACTA AGAAGCCGCT GCCGCCCCTG 
CGACACCTGG ACGGCCTGGC CCAAGACTAT GCTTCCGATT CGGGCTGCTT TGACGACGAC 
GATGCACCGT CATCCCTGGC TGCGGCCGCG GCCACCGGGG AGCCAGCCAG CCCTGCCGCG 
CTGTCCCTCT TCCTGGGCAG CCCCTTCCTG CAGGGGCAGG CGCTGGCTCG CACCCCGCCC 
ACCCGCCGAG GCGGCAAGGA GGCAGAGAAG GACACCAGTG GGGAGGCAGC CGTGGCGGCC 
AAGGCCAAGG CCAAGCTGGC GCTTGCAGTG GCGCGCATCG ACCAGCTGGT GGAGGACATC 
TCCGCCCTGC ACACCTCGTC CGACGATAGC TTCAGCCTCA GCTCTGGAGA CCCGGGACAG 
GAGGCGCCAC GGGAGGGCCG CGCCCAGTCC TGCTCGCCAT GCCGCGGCCC GGAGGGCGGG 
CGGCGAGAGG CAGGAAGCCG GGCGCACCCG CTGCTGCGGC TCAAGGCGGC CCACGCCAGC 
CTCTCCAACG ACAGCCTCAA CAGCGGCAGT GCCAGCGACG GGTACTGCCC ACGCGAACAT 
ATGCTGCCCT GCCCGCTGGC CGCACTGGCT TCGCGCCGCG AGGACCCCAG GTGTGGGCAG 
CCTCGGCCCA GCCGGCTTGA CCTTGACCTG CCCGGCTGCC AGGCCGAGCC CCCGGCCCGC 
GAGGCCACCT CCGCCGACGC CCGCGTGCGC ACCATCAAGC TGTCGCCTAC CTATCAGCAC 
GTGCCACTGC TTGAGGGTGC CTCAAGGGCG GGTGCAGAGC CCCTCGCGGG GCCTGGAATC 
TCTCCAGGGG CCCGGAAGCA GGCCTGGCTG CCGGCAGACC ACCTGAGCAA GGTTCCCGAG 
AAGCTGGCGG CTGCCCCGCT GTCTGTGGCC AGCAAGGCAC TGCAGAAACT GGCGGCGCAA 
GAGGGGCCAC TCTCGCTGTC CCGATGCAGC TCCCTTTCCT CGCTGTCCTC GGCCGGCCGC 
CCAGGCCCCA GCGAGGGTGG TGACCTGGAT GACAGTGACT CCTCCCTGGA GGGGCTGGAG 
GAGGCCGGCC CCAGCGAGGC TGAGCTGGAC AGCACGTGGC GGGCGCCCGG GGCCACCTCG 
CTGCCCGTAG CCATTCCGGC TCCCCGGCGT AACCGAGGCC GGGGCCTGGG GGTGGAAGAC 
GCCACGCCGT CCAGCTCGTC GGAGAACTAC GTGCAGGAGA CACCGCTTGT GCTGAGCCGC 
TGCAGCTCTG TGAGCTCGCT GGGCAGCTTC GAGAGCCCGT CCATCGCCAG CTCCATCCCC 
AGTGAACCTT GCAGCGGGCA GGGCAGCGGC ACCATCAGCC CTAGCGAGCT GCCCGACAGC 
CCCGGACAGA CCATGCCTCC CAGCCGGAGC AAGACGCCAC CGCTGGCGCC CGCGCCACAG 
GGTCCCCCCG AGGCCACCCA GTTCAGCCTG CAGTGGGAGA GCTACGTGAA GCGCTTCCTG 
GACATCGCCG ACTGCCGGGA GCGCTGCCGG CTGCCATCTG AGCTGGACGC AGGCAGCGTG 
CGCTTTACCG TGGAGAAGCC AGACGAGAAC TTCTCGTGCG CCTCCAGCCT CAGCGCGCTG 
GCCTTGCACG AGCACTACGT GCAGCAGGAC GTGGAGCTGC GGCTGCTGCC CTCGGCCTGC 
CCCGAGCGCG GCGGGGGCGC CGGGGGCGCC GGCCTCCACT TTGCAGGGCA CCGGCGGCGG 
GAGGAGGGGC CGGCGCCCAC GGGTTCTCGC CCTCGCGGCG CCGCGGACCA GGAGCTGGAA 
CTGCTGCGGG AGTGCCTGGG AGCCGCCGTG CCTGCCCGGC TGCGCAAGGT GGCCTCCGCG 
CTGGTGCCAG GTCGCCGCGC ACTCCCCGTG CCCGTCTACA TGTTGGTGCC CGCCCCGGCC 
CCGGCCCAGG AGGACGACTC CTGCACTGAC TCCGCGGAGG GCACGCCGGT CAACTTCTCT 
AGCGCCGCCT CGCTCAGCGA CGAGACGCTG CAGGGACCCC CCAGGGACCA GCCCGGGGGA 
CCAGCGGGCA GGCAAAGACC CACCGGCCGC CCCACCTCTG CCAGACAGGC CATGGGGCAC 
CGGCACAAGG CGGGAGGCGC CGGCCGCAGC GCGGAGCAGT CTCGGGGCGC GGGCAAGAAC 
AGAGCAGGGC TGGAGCTGCC CCTGGGCCGG CCCCCGAGCG CCCCCGCAGA CAAGGACGGC 
TCAAAGCCCG GCCGGACCCG CGGGGACGGG GCGCTCCAGT CGCTGTGCCT CACGACGCCC 
ACTGAGGAGG CCGTGTACTG CTTCTACGGC AACGACTCGG ACGAGGAGCC CCCGGCGGCC 
GCGCCCACGC CAACCCACCG GCGCACATCG GCCATCCCTC GCGCTTTTAC GCGGGAGCGT 
CCGCAGGGCC GGAAGGAGGC CCCTGCCCCG TCCAAGGCTG CACCAGCTGC CCCGCCGCCC 
GCCCGGACCC AGCCCAGCCT CATTGCTGAC GAGACCCCGC CCTGCTACTC CCTGAGCTCC 
TCCGCCAGCT CCCTCAGCGA GCCCGAGCCC TCGGAGCCGC CGGCCGTCCA TCCACGAGGC 
CGGGAGCCCG CGGTCACCAA GGACCCGGGC CCAGGAGGCG GACGCGACAG CTCGCCCAGC 
CCGCGGGCCG CGGAGGAGCT TCTGCAGCGG TGCATCAGCT CGGCCCTGCC CAGGCGCCGG 
CCCCCCGTGT CTGGCCTGCG GCGCCGCAAG CCCCGAGCCA CCCGGCTGGA TGAGCGGCCC 
GCAGAGGGGT CCCGGGAACG CGGCGAGGAG GCAGCGGGCT CGGACCGGGC CTCCGACCTG 
GATAGCGTGG AGTGGCGCGC CATCCAGGAG GGCGCCAATT CAATTGTCAC GTGGCTGCAC 
CAGGCAGCAG CTGCCACGCG GGAGGCCTCG TCCGAGTCCG ACTCCATCCT GTCCTTCGTA 
TCCGGGCTGT CAGTGGGATC CACCCTACAG CCCCCCAAGC ACAGGAAGGG ACGACAGGCG 
GAGGGAGAAA TGGGCAGTGC CCGGCGGCCA GAGAAAAGGG GCGCAGCCTC AGTCAAGACC 
AGCGGGAGCC CCCGTTCCCC TGCAGGCCCC GAGAAGCCAC GTGGCACACA GAAGACCACG 
CCCGGGGTGC CAGCTGTGCT CCGGGGACGA ACAGTGATCT ACGTCCCCAG CCCGGCACCC 
CGTGCCCAGC CCAAAGGGAC CCCCGGCCCC CGCGCCACAC CGCGGAAGGT GGCGCCCCCT 
TGCCTGGCAC AGCCCGCGGC TCCAGCCAAA GTCCCGAGCC CCGGGCAGCA GCGGTCGCGG 
AGCCTACACC GGCCTGCCAA GACCTCGGAG CTGGCGACGC TGAGCCAGCC CCCCAGAAGC 
GCCACACCGC CCGCCCGCCT CGCCAAGACC CCCTCCTCCA GCTCCTCCCA GACCTCGCCC 
GCCTCCCAGC CCCTGCCCAG AAAGCGCCCC CCGGTCACCC AGGCTGCTGG GGCCCTGCCC 
GGCCCCGGAG CCTCCCCGGT GCCCAAAACG CCGGCGCGCA CCCTTCTGGC GAAGCAGCAC 
AAGACGCAGA GATCGCCCGT GCGGATCCCG TTCATGCAGA GGCCGGCCCG GCGTGGGCCG 
CCACCGCTGG CTCGGGCAGT CCCGGAGCCG GGCCCCAGGG GCCGGGCGGG GACCGAGGCG 
GGCCCGGGGG CGCGCGGGGG CCGCCTGGGC CTGGTGCGTG TGGCCTCAGC CCTCTCCAGC 
GGCAGCGAGT CCTCCGACCG CTCGGGCTTC CGGCGACAGC TAACCTTCAT CAAGGAGTCG 
CCGGGCTTGC GGCGCCGCCG CTCCGAGCTG TCCTCGGCCG AGTCCGCGGC CTCTGCCCCC 
CAGGGCGCCT CGCCCCGCCG CGGCCGGCCC GCGCTGCCCG CCGTCTTCCT CTGCTCCTCG 
CGCTGCGAAG AGCTCCGAGC GGCACCCCGG CAGGGCCCGG CCCCGGCCCG GCAGCGGCCC 
CCCGCGGCCC GACCCAGCCC TGGCGAGCGC CCTGCCCGGC GCACCACCTC CGAGAGCCCG 
TCCCGCCTGC CTGTGCGCGC GCCCGCCGCC CGGCCGGAGA CTGTCAAGCG CTACGCGTCG 
CTGCCGCACA TCAGCGTGGC CCGCAGGCCC GACGGCGCCG TCCCCGCGGC CCCTGCCTCA 
GCCGACGCCG CGCGCCGCAG CAGCGACGGG GAGCCCCGGC CGCTCCCCAG GGTGGCCGCG 
CCGGGCACGA CCTGGCGGCG CATCCGAGAT GAGGACGTGC CCCACATCCT GCGCAGCACG 
CTTCCCGCCA CGGCCCTGCC ACTGCGGGGC TCCACGCCCG AGGACGCCCC GGCCGGGCCC 
CCGCCGCGCA AGACCAGCGA CGCCGTGGTC CAGACCGAGG AGGTCGCCGC CCCCAAGACC 
AACTCCAGCA CGTCCCCGAG CCTGGAGACC AGGGAGCCCC CCGGGGCCCC CGCCGGCGGC 
CAGCTCTCCC TCCTCGGCAG CGACGTGGAC GGTCCCAGCC TCGCCAAGGC TCCCATCTCC 
GCACCCTTCG TGCACGAGGG CCTGGGGGTC GCCGTGGGGG GCTTCCCCGC CAGCCGGCAC 
GGCTCCCCCA GCCGCTCGGC CCGAGTACCC CCCTTCAACT ATGTGCCCAG CCCCATGGTG 
GTCGCAGCCA CCACCGACTC GGCCGCGGAG AAAGCCCCGG CCACTGCCTC CGCCACCCTC 
CTGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APC2 ( XM_006722609.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722672.1 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X5, mRNA.

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CloneID	OHu05000
Clone ID Related Accession (Same CDS sequence)	NM_005883.2 , XM_006722609.3
Accession Version	XM_006722609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCT CCGTGGCGCC CTACGAGCAG CTGGTGAGGC AGGTGGAGGC CTTGAAGGCT 
GAGAACAGCC ACCTGAGGCA GGAGCTAAGG GACAACTCCA GCCACCTGTC CAAGCTGGAG 
ACAGAGACGT CGGGCATGAA GGAGGTCCTG AAGCACCTAC AGGGAAAACT GGAGCAGGAG 
GCCCGAGTGC TGGTGTCCTC GGGGCAGACG GAGGTGCTGG AGCAGCTGAA GGCCCTACAG 
ATGGACATCA CCAGCCTGTA CAACCTCAAG TTCCAGCCGC CCACCCTGGG CCCGGAGCCT 
GCCGCCCGGA CCCCCGAGGG CAGCCCAGTA CACGGCTCCG GGCCCTCCAA GGACAGCTTT 
GGGGAGCTGA GCCGGGCCAC CATCCGGCTG CTGGAGGAAC TGGACCGGGA ACGGTGTTTC 
CTGCTGAATG AGATTGAGAA GGAGGAGAAG GAGAAGCTCT GGTACTACTC TCAGCTGCAG 
GGCCTGTCCA AGCGCCTGGA CGAGCTGCCG CACGTGGAGA CGCAGTTCTC GATGCAGATG 
GACCTGATCC GGCAGCAGCT TGAGTTCGAG GCCCAGCACA TCCGCTCGCT GATGGAGGAG 
CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCACAGA TCCGCGCCTC GCGCCTGGAG 
CAGATTGACA AGGAGCTGCT GGAGGCGCAG GACCGAGTGC AGCAGACGGA GCCCCAGGCC 
TTGCTGGCGG TGAAGTCGGT GCCGGTGGAC GAGGACCCCG AGACAGAGGT CCCCACACAC 
CCTGAGGATG GCACCCCTCA GCCGGGCAAC AGCAAGGTGG AGGTGGTCTT CTGGCTGTTG 
TCCATGTTGG CGACGCGCGA CCAGGAGGAT ACAGCGCGCA CGCTGCTGGC CATGTCCAGC 
TCGCCCGAGA GCTGCGTGGC CATGCGCCGC TCGGGCTGTC TGCCTCTGCT GCTGCAAATC 
CTCCACGGCA CCGAGGCCGC GGCCGGGGGT CGCGCCGGGG CCCCAGGGGC ACCGGGCGCC 
AAGGACGCAC GCATGCGCGC CAACGCGGCG CTGCACAACA TCGTCTTCTC GCAGCCGGAC 
CAGGGCCTGG CGCGCAAGGA GATGCGCGTC CTGCACGTGC TGGAGCAGAT CCGGGCCTAC 
TGCGAGACCT GCTGGGACTG GCTGCAGGCC CGAGACGGCG GGCCCGAGGG AGGTGGCGCC 
GGCAGCGCCC CGATCCCCAT CGAGCCGCAG ATCTGCCAGG CCACCTGTGC TGTTATGAAG 
CTGTCCTTTG ATGAGGAGTA CCGCCGTGCC ATGAACGAGC TAGGTGGGCT GCAGGCCGTG 
GCAGAGCTGC TGCAGGTTGA CTATGAGATG CACAAGATGA CCCGGGACCC GCTGAACCTG 
GCGCTGCGCC GCTACGCGGG CATGACCCTC ACCAACCTCA CCTTTGGGGA CGTTGCCAAC 
AAGGCCACCC TGTGTGCGCG CCGCGGCTGC ATGGAGGCCA TCGTGGCCCA GCTGGCCTCC 
GACAGTGAGG AGCTCCACCA GGTGGTGTCC AGCATCCTTC GGAACTTGTC CTGGAGGGCC 
GACATCAACA GCAAGAAGGT GCTGAGGGAG GCGGGCAGCG TGACTGCCCT GGTGCAGTGT 
GTCCTGCGGG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TGAGCGCCCT GTGGAATCTG 
TCTGCACACA GCACAGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGCGC CCTGGGCTTC 
CTGGTGAGCA CCCTGACCTA CAAGTGTCAG AGCAACTCGC TGGCCATCAT CGAGAGCGGC 
GGCGGCATCC TCCGCAATGT GTCCAGCCTC GTCGCCACCC GTGAGGACTA CAGGCAGGTG 
CTCCGGGATC ACAACTGTCT GCAGACGCTG CTGCAGCATC TGACTTCGCA CAGCCTGACC 
ATCGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCGC CCGTGACCAG 
GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGTA ATCTGGTGCA CTCCAAGCAC 
AAGATGATCG CCATGGGCAG CGCCGCCGCC CTGCGCAACC TGCTGGCCCA TCGGCCCGCC 
AAGCACCAGG CGGCCGCCAC CGCCGTGTCC CCAGGCAGCT GCGTGCCCAG CCTGTACGTG 
CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACGCACGGC ACCTCGCGCA GGCGCTGGAG 
CACCTGGAGA AGCAGGGCCC GCCGGCAGCC GAGGCCGCCA CTAAGAAGCC GCTGCCGCCC 
CTGCGACACC TGGACGGCCT GGCCCAAGAC TATGCTTCCG ATTCGGGCTG CTTTGACGAC 
GACGATGCAC CGTCATCCCT GGCTGCGGCC GCGGCCACCG GGGAGCCAGC CAGCCCTGCC 
GCGCTGTCCC TCTTCCTGGG CAGCCCCTTC CTGCAGGGGC AGGCGCTGGC TCGCACCCCG 
CCCACCCGCC GAGGCGGCAA GGAGGCAGAG AAGGACACCA GTGGGGAGGC AGCCGTGGCG 
GCCAAGGCCA AGGCCAAGCT GGCGCTTGCA GTGGCGCGCA TCGACCAGCT GGTGGAGGAC 
ATCTCCGCCC TGCACACCTC GTCCGACGAT AGCTTCAGCC TCAGCTCTGG AGACCCGGGA 
CAGGAGGCGC CACGGGAGGG CCGCGCCCAG TCCTGCTCGC CATGCCGCGG CCCGGAGGGC 
GGGCGGCGAG AGGCAGGAAG CCGGGCGCAC CCGCTGCTGC GGCTCAAGGC GGCCCACGCC 
AGCCTCTCCA ACGACAGCCT CAACAGCGGC AGTGCCAGCG ACGGGTACTG CCCACGCGAA 
CATATGCTGC CCTGCCCGCT GGCCGCACTG GCTTCGCGCC GCGAGGACCC CAGGTGTGGG 
CAGCCTCGGC CCAGCCGGCT TGACCTTGAC CTGCCCGGCT GCCAGGCCGA GCCCCCGGCC 
CGCGAGGCCA CCTCCGCCGA CGCCCGCGTG CGCACCATCA AGCTGTCGCC TACCTATCAG 
CACGTGCCAC TGCTTGAGGG TGCCTCAAGG GCGGGTGCAG AGCCCCTCGC GGGGCCTGGA 
ATCTCTCCAG GGGCCCGGAA GCAGGCCTGG CTGCCGGCAG ACCACCTGAG CAAGGTTCCC 
GAGAAGCTGG CGGCTGCCCC GCTGTCTGTG GCCAGCAAGG CACTGCAGAA ACTGGCGGCG 
CAAGAGGGGC CACTCTCGCT GTCCCGATGC AGCTCCCTTT CCTCGCTGTC CTCGGCCGGC 
CGCCCAGGCC CCAGCGAGGG TGGTGACCTG GATGACAGTG ACTCCTCCCT GGAGGGGCTG 
GAGGAGGCCG GCCCCAGCGA GGCTGAGCTG GACAGCACGT GGCGGGCGCC CGGGGCCACC 
TCGCTGCCCG TAGCCATTCC GGCTCCCCGG CGTAACCGAG GCCGGGGCCT GGGGGTGGAA 
GACGCCACGC CGTCCAGCTC GTCGGAGAAC TACGTGCAGG AGACACCGCT TGTGCTGAGC 
CGCTGCAGCT CTGTGAGCTC GCTGGGCAGC TTCGAGAGCC CGTCCATCGC CAGCTCCATC 
CCCAGTGAAC CTTGCAGCGG GCAGGGCAGC GGCACCATCA GCCCTAGCGA GCTGCCCGAC 
AGCCCCGGAC AGACCATGCC TCCCAGCCGG AGCAAGACGC CACCGCTGGC GCCCGCGCCA 
CAGGGTCCCC CCGAGGCCAC CCAGTTCAGC CTGCAGTGGG AGAGCTACGT GAAGCGCTTC 
CTGGACATCG CCGACTGCCG GGAGCGCTGC CGGCTGCCAT CTGAGCTGGA CGCAGGCAGC 
GTGCGCTTTA CCGTGGAGAA GCCAGACGAG AACTTCTCGT GCGCCTCCAG CCTCAGCGCG 
CTGGCCTTGC ACGAGCACTA CGTGCAGCAG GACGTGGAGC TGCGGCTGCT GCCCTCGGCC 
TGCCCCGAGC GCGGCGGGGG CGCCGGGGGC GCCGGCCTCC ACTTTGCAGG GCACCGGCGG 
CGGGAGGAGG GGCCGGCGCC CACGGGTTCT CGCCCTCGCG GCGCCGCGGA CCAGGAGCTG 
GAACTGCTGC GGGAGTGCCT GGGAGCCGCC GTGCCTGCCC GGCTGCGCAA GGTGGCCTCC 
GCGCTGGTGC CAGGTCGCCG CGCACTCCCC GTGCCCGTCT ACATGTTGGT GCCCGCCCCG 
GCCCCGGCCC AGGAGGACGA CTCCTGCACT GACTCCGCGG AGGGCACGCC GGTCAACTTC 
TCTAGCGCCG CCTCGCTCAG CGACGAGACG CTGCAGGGAC CCCCCAGGGA CCAGCCCGGG 
GGACCAGCGG GCAGGCAAAG ACCCACCGGC CGCCCCACCT CTGCCAGACA GGCCATGGGG 
CACCGGCACA AGGCGGGAGG CGCCGGCCGC AGCGCGGAGC AGTCTCGGGG CGCGGGCAAG 
AACAGAGCAG GGCTGGAGCT GCCCCTGGGC CGGCCCCCGA GCGCCCCCGC AGACAAGGAC 
GGCTCAAAGC CCGGCCGGAC CCGCGGGGAC GGGGCGCTCC AGTCGCTGTG CCTCACGACG 
CCCACTGAGG AGGCCGTGTA CTGCTTCTAC GGCAACGACT CGGACGAGGA GCCCCCGGCG 
GCCGCGCCCA CGCCAACCCA CCGGCGCACA TCGGCCATCC CTCGCGCTTT TACGCGGGAG 
CGTCCGCAGG GCCGGAAGGA GGCCCCTGCC CCGTCCAAGG CTGCACCAGC TGCCCCGCCG 
CCCGCCCGGA CCCAGCCCAG CCTCATTGCT GACGAGACCC CGCCCTGCTA CTCCCTGAGC 
TCCTCCGCCA GCTCCCTCAG CGAGCCCGAG CCCTCGGAGC CGCCGGCCGT CCATCCACGA 
GGCCGGGAGC CCGCGGTCAC CAAGGACCCG GGCCCAGGAG GCGGACGCGA CAGCTCGCCC 
AGCCCGCGGG CCGCGGAGGA GCTTCTGCAG CGGTGCATCA GCTCGGCCCT GCCCAGGCGC 
CGGCCCCCCG TGTCTGGCCT GCGGCGCCGC AAGCCCCGAG CCACCCGGCT GGATGAGCGG 
CCCGCAGAGG GGTCCCGGGA ACGCGGCGAG GAGGCAGCGG GCTCGGACCG GGCCTCCGAC 
CTGGATAGCG TGGAGTGGCG CGCCATCCAG GAGGGCGCCA ATTCAATTGT CACGTGGCTG 
CACCAGGCAG CAGCTGCCAC GCGGGAGGCC TCGTCCGAGT CCGACTCCAT CCTGTCCTTC 
GTATCCGGGC TGTCAGTGGG ATCCACCCTA CAGCCCCCCA AGCACAGGAA GGGACGACAG 
GCGGAGGGAG AAATGGGCAG TGCCCGGCGG CCAGAGAAAA GGGGCGCAGC CTCAGTCAAG 
ACCAGCGGGA GCCCCCGTTC CCCTGCAGGC CCCGAGAAGC CACGTGGCAC ACAGAAGACC 
ACGCCCGGGG TGCCAGCTGT GCTCCGGGGA CGAACAGTGA TCTACGTCCC CAGCCCGGCA 
CCCCGTGCCC AGCCCAAAGG GACCCCCGGC CCCCGCGCCA CACCGCGGAA GGTGGCGCCC 
CCTTGCCTGG CACAGCCCGC GGCTCCAGCC AAAGTCCCGA GCCCCGGGCA GCAGCGGTCG 
CGGAGCCTAC ACCGGCCTGC CAAGACCTCG GAGCTGGCGA CGCTGAGCCA GCCCCCCAGA 
AGCGCCACAC CGCCCGCCCG CCTCGCCAAG ACCCCCTCCT CCAGCTCCTC CCAGACCTCG 
CCCGCCTCCC AGCCCCTGCC CAGAAAGCGC CCCCCGGTCA CCCAGGCTGC TGGGGCCCTG 
CCCGGCCCCG GAGCCTCCCC GGTGCCCAAA ACGCCGGCGC GCACCCTTCT GGCGAAGCAG 
CACAAGACGC AGAGATCGCC CGTGCGGATC CCGTTCATGC AGAGGCCGGC CCGGCGTGGG 
CCGCCACCGC TGGCTCGGGC AGTCCCGGAG CCGGGCCCCA GGGGCCGGGC GGGGACCGAG 
GCGGGCCCGG GGGCGCGCGG GGGCCGCCTG GGCCTGGTGC GTGTGGCCTC AGCCCTCTCC 
AGCGGCAGCG AGTCCTCCGA CCGCTCGGGC TTCCGGCGAC AGCTAACCTT CATCAAGGAG 
TCGCCGGGCT TGCGGCGCCG CCGCTCCGAG CTGTCCTCGG CCGAGTCCGC GGCCTCTGCC 
CCCCAGGGCG CCTCGCCCCG CCGCGGCCGG CCCGCGCTGC CCGCCGTCTT CCTCTGCTCC 
TCGCGCTGCG AAGAGCTCCG AGCGGCACCC CGGCAGGGCC CGGCCCCGGC CCGGCAGCGG 
CCCCCCGCGG CCCGACCCAG CCCTGGCGAG CGCCCTGCCC GGCGCACCAC CTCCGAGAGC 
CCGTCCCGCC TGCCTGTGCG CGCGCCCGCC GCCCGGCCGG AGACTGTCAA GCGCTACGCG 
TCGCTGCCGC ACATCAGCGT GGCCCGCAGG CCCGACGGCG CCGTCCCCGC GGCCCCTGCC 
TCAGCCGACG CCGCGCGCCG CAGCAGCGAC GGGGAGCCCC GGCCGCTCCC CAGGGTGGCC 
GCGCCGGGCA CGACCTGGCG GCGCATCCGA GATGAGGACG TGCCCCACAT CCTGCGCAGC 
ACGCTTCCCG CCACGGCCCT GCCACTGCGG GGCTCCACGC CCGAGGACGC CCCGGCCGGG 
CCCCCGCCGC GCAAGACCAG CGACGCCGTG GTCCAGACCG AGGAGGTCGC CGCCCCCAAG 
ACCAACTCCA GCACGTCCCC GAGCCTGGAG ACCAGGGAGC CCCCCGGGGC CCCCGCCGGC 
GGCCAGCTCT CCCTCCTCGG CAGCGACGTG GACGGTCCCA GCCTCGCCAA GGCTCCCATC 
TCCGCACCCT TCGTGCACGA GGGCCTGGGG GTCGCCGTGG GGGGCTTCCC CGCCAGCCGG 
CACGGCTCCC CCAGCCGCTC GGCCCGAGTA CCCCCCTTCA ACTATGTGCC CAGCCCCATG 
GTGGTCGCAG CCACCACCGA CTCGGCCGCG GAGAAAGCCC CGGCCACTGC CTCCGCCACC 
CTCCTGGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722672.1
CDS	163..7074
Translation	MASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETQFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	XM_006722609.3
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722609.3

ORF Insert Sequence:

ATGGCGAGCT CCGTGGCGCC CTACGAGCAG CTGGTGAGGC AGGTGGAGGC CTTGAAGGCT 
GAGAACAGCC ACCTGAGGCA GGAGCTAAGG GACAACTCCA GCCACCTGTC CAAGCTGGAG 
ACAGAGACGT CGGGCATGAA GGAGGTCCTG AAGCACCTAC AGGGAAAACT GGAGCAGGAG 
GCCCGAGTGC TGGTGTCCTC GGGGCAGACG GAGGTGCTGG AGCAGCTGAA GGCCCTACAG 
ATGGACATCA CCAGCCTGTA CAACCTCAAG TTCCAGCCGC CCACCCTGGG CCCGGAGCCT 
GCCGCCCGGA CCCCCGAGGG CAGCCCAGTA CACGGCTCCG GGCCCTCCAA GGACAGCTTT 
GGGGAGCTGA GCCGGGCCAC CATCCGGCTG CTGGAGGAAC TGGACCGGGA ACGGTGTTTC 
CTGCTGAATG AGATTGAGAA GGAGGAGAAG GAGAAGCTCT GGTACTACTC TCAGCTGCAG 
GGCCTGTCCA AGCGCCTGGA CGAGCTGCCG CACGTGGAGA CGCAGTTCTC GATGCAGATG 
GACCTGATCC GGCAGCAGCT TGAGTTCGAG GCCCAGCACA TCCGCTCGCT GATGGAGGAG 
CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCACAGA TCCGCGCCTC GCGCCTGGAG 
CAGATTGACA AGGAGCTGCT GGAGGCGCAG GACCGAGTGC AGCAGACGGA GCCCCAGGCC 
TTGCTGGCGG TGAAGTCGGT GCCGGTGGAC GAGGACCCCG AGACAGAGGT CCCCACACAC 
CCTGAGGATG GCACCCCTCA GCCGGGCAAC AGCAAGGTGG AGGTGGTCTT CTGGCTGTTG 
TCCATGTTGG CGACGCGCGA CCAGGAGGAT ACAGCGCGCA CGCTGCTGGC CATGTCCAGC 
TCGCCCGAGA GCTGCGTGGC CATGCGCCGC TCGGGCTGTC TGCCTCTGCT GCTGCAAATC 
CTCCACGGCA CCGAGGCCGC GGCCGGGGGT CGCGCCGGGG CCCCAGGGGC ACCGGGCGCC 
AAGGACGCAC GCATGCGCGC CAACGCGGCG CTGCACAACA TCGTCTTCTC GCAGCCGGAC 
CAGGGCCTGG CGCGCAAGGA GATGCGCGTC CTGCACGTGC TGGAGCAGAT CCGGGCCTAC 
TGCGAGACCT GCTGGGACTG GCTGCAGGCC CGAGACGGCG GGCCCGAGGG AGGTGGCGCC 
GGCAGCGCCC CGATCCCCAT CGAGCCGCAG ATCTGCCAGG CCACCTGTGC TGTTATGAAG 
CTGTCCTTTG ATGAGGAGTA CCGCCGTGCC ATGAACGAGC TAGGTGGGCT GCAGGCCGTG 
GCAGAGCTGC TGCAGGTTGA CTATGAGATG CACAAGATGA CCCGGGACCC GCTGAACCTG 
GCGCTGCGCC GCTACGCGGG CATGACCCTC ACCAACCTCA CCTTTGGGGA CGTTGCCAAC 
AAGGCCACCC TGTGTGCGCG CCGCGGCTGC ATGGAGGCCA TCGTGGCCCA GCTGGCCTCC 
GACAGTGAGG AGCTCCACCA GGTGGTGTCC AGCATCCTTC GGAACTTGTC CTGGAGGGCC 
GACATCAACA GCAAGAAGGT GCTGAGGGAG GCGGGCAGCG TGACTGCCCT GGTGCAGTGT 
GTCCTGCGGG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TGAGCGCCCT GTGGAATCTG 
TCTGCACACA GCACAGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGCGC CCTGGGCTTC 
CTGGTGAGCA CCCTGACCTA CAAGTGTCAG AGCAACTCGC TGGCCATCAT CGAGAGCGGC 
GGCGGCATCC TCCGCAATGT GTCCAGCCTC GTCGCCACCC GTGAGGACTA CAGGCAGGTG 
CTCCGGGATC ACAACTGTCT GCAGACGCTG CTGCAGCATC TGACTTCGCA CAGCCTGACC 
ATCGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCGC CCGTGACCAG 
GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGTA ATCTGGTGCA CTCCAAGCAC 
AAGATGATCG CCATGGGCAG CGCCGCCGCC CTGCGCAACC TGCTGGCCCA TCGGCCCGCC 
AAGCACCAGG CGGCCGCCAC CGCCGTGTCC CCAGGCAGCT GCGTGCCCAG CCTGTACGTG 
CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACGCACGGC ACCTCGCGCA GGCGCTGGAG 
CACCTGGAGA AGCAGGGCCC GCCGGCAGCC GAGGCCGCCA CTAAGAAGCC GCTGCCGCCC 
CTGCGACACC TGGACGGCCT GGCCCAAGAC TATGCTTCCG ATTCGGGCTG CTTTGACGAC 
GACGATGCAC CGTCATCCCT GGCTGCGGCC GCGGCCACCG GGGAGCCAGC CAGCCCTGCC 
GCGCTGTCCC TCTTCCTGGG CAGCCCCTTC CTGCAGGGGC AGGCGCTGGC TCGCACCCCG 
CCCACCCGCC GAGGCGGCAA GGAGGCAGAG AAGGACACCA GTGGGGAGGC AGCCGTGGCG 
GCCAAGGCCA AGGCCAAGCT GGCGCTTGCA GTGGCGCGCA TCGACCAGCT GGTGGAGGAC 
ATCTCCGCCC TGCACACCTC GTCCGACGAT AGCTTCAGCC TCAGCTCTGG AGACCCGGGA 
CAGGAGGCGC CACGGGAGGG CCGCGCCCAG TCCTGCTCGC CATGCCGCGG CCCGGAGGGC 
GGGCGGCGAG AGGCAGGAAG CCGGGCGCAC CCGCTGCTGC GGCTCAAGGC GGCCCACGCC 
AGCCTCTCCA ACGACAGCCT CAACAGCGGC AGTGCCAGCG ACGGGTACTG CCCACGCGAA 
CATATGCTGC CCTGCCCGCT GGCCGCACTG GCTTCGCGCC GCGAGGACCC CAGGTGTGGG 
CAGCCTCGGC CCAGCCGGCT TGACCTTGAC CTGCCCGGCT GCCAGGCCGA GCCCCCGGCC 
CGCGAGGCCA CCTCCGCCGA CGCCCGCGTG CGCACCATCA AGCTGTCGCC TACCTATCAG 
CACGTGCCAC TGCTTGAGGG TGCCTCAAGG GCGGGTGCAG AGCCCCTCGC GGGGCCTGGA 
ATCTCTCCAG GGGCCCGGAA GCAGGCCTGG CTGCCGGCAG ACCACCTGAG CAAGGTTCCC 
GAGAAGCTGG CGGCTGCCCC GCTGTCTGTG GCCAGCAAGG CACTGCAGAA ACTGGCGGCG 
CAAGAGGGGC CACTCTCGCT GTCCCGATGC AGCTCCCTTT CCTCGCTGTC CTCGGCCGGC 
CGCCCAGGCC CCAGCGAGGG TGGTGACCTG GATGACAGTG ACTCCTCCCT GGAGGGGCTG 
GAGGAGGCCG GCCCCAGCGA GGCTGAGCTG GACAGCACGT GGCGGGCGCC CGGGGCCACC 
TCGCTGCCCG TAGCCATTCC GGCTCCCCGG CGTAACCGAG GCCGGGGCCT GGGGGTGGAA 
GACGCCACGC CGTCCAGCTC GTCGGAGAAC TACGTGCAGG AGACACCGCT TGTGCTGAGC 
CGCTGCAGCT CTGTGAGCTC GCTGGGCAGC TTCGAGAGCC CGTCCATCGC CAGCTCCATC 
CCCAGTGAAC CTTGCAGCGG GCAGGGCAGC GGCACCATCA GCCCTAGCGA GCTGCCCGAC 
AGCCCCGGAC AGACCATGCC TCCCAGCCGG AGCAAGACGC CACCGCTGGC GCCCGCGCCA 
CAGGGTCCCC CCGAGGCCAC CCAGTTCAGC CTGCAGTGGG AGAGCTACGT GAAGCGCTTC 
CTGGACATCG CCGACTGCCG GGAGCGCTGC CGGCTGCCAT CTGAGCTGGA CGCAGGCAGC 
GTGCGCTTTA CCGTGGAGAA GCCAGACGAG AACTTCTCGT GCGCCTCCAG CCTCAGCGCG 
CTGGCCTTGC ACGAGCACTA CGTGCAGCAG GACGTGGAGC TGCGGCTGCT GCCCTCGGCC 
TGCCCCGAGC GCGGCGGGGG CGCCGGGGGC GCCGGCCTCC ACTTTGCAGG GCACCGGCGG 
CGGGAGGAGG GGCCGGCGCC CACGGGTTCT CGCCCTCGCG GCGCCGCGGA CCAGGAGCTG 
GAACTGCTGC GGGAGTGCCT GGGAGCCGCC GTGCCTGCCC GGCTGCGCAA GGTGGCCTCC 
GCGCTGGTGC CAGGTCGCCG CGCACTCCCC GTGCCCGTCT ACATGTTGGT GCCCGCCCCG 
GCCCCGGCCC AGGAGGACGA CTCCTGCACT GACTCCGCGG AGGGCACGCC GGTCAACTTC 
TCTAGCGCCG CCTCGCTCAG CGACGAGACG CTGCAGGGAC CCCCCAGGGA CCAGCCCGGG 
GGACCAGCGG GCAGGCAAAG ACCCACCGGC CGCCCCACCT CTGCCAGACA GGCCATGGGG 
CACCGGCACA AGGCGGGAGG CGCCGGCCGC AGCGCGGAGC AGTCTCGGGG CGCGGGCAAG 
AACAGAGCAG GGCTGGAGCT GCCCCTGGGC CGGCCCCCGA GCGCCCCCGC AGACAAGGAC 
GGCTCAAAGC CCGGCCGGAC CCGCGGGGAC GGGGCGCTCC AGTCGCTGTG CCTCACGACG 
CCCACTGAGG AGGCCGTGTA CTGCTTCTAC GGCAACGACT CGGACGAGGA GCCCCCGGCG 
GCCGCGCCCA CGCCAACCCA CCGGCGCACA TCGGCCATCC CTCGCGCTTT TACGCGGGAG 
CGTCCGCAGG GCCGGAAGGA GGCCCCTGCC CCGTCCAAGG CTGCACCAGC TGCCCCGCCG 
CCCGCCCGGA CCCAGCCCAG CCTCATTGCT GACGAGACCC CGCCCTGCTA CTCCCTGAGC 
TCCTCCGCCA GCTCCCTCAG CGAGCCCGAG CCCTCGGAGC CGCCGGCCGT CCATCCACGA 
GGCCGGGAGC CCGCGGTCAC CAAGGACCCG GGCCCAGGAG GCGGACGCGA CAGCTCGCCC 
AGCCCGCGGG CCGCGGAGGA GCTTCTGCAG CGGTGCATCA GCTCGGCCCT GCCCAGGCGC 
CGGCCCCCCG TGTCTGGCCT GCGGCGCCGC AAGCCCCGAG CCACCCGGCT GGATGAGCGG 
CCCGCAGAGG GGTCCCGGGA ACGCGGCGAG GAGGCAGCGG GCTCGGACCG GGCCTCCGAC 
CTGGATAGCG TGGAGTGGCG CGCCATCCAG GAGGGCGCCA ATTCAATTGT CACGTGGCTG 
CACCAGGCAG CAGCTGCCAC GCGGGAGGCC TCGTCCGAGT CCGACTCCAT CCTGTCCTTC 
GTATCCGGGC TGTCAGTGGG ATCCACCCTA CAGCCCCCCA AGCACAGGAA GGGACGACAG 
GCGGAGGGAG AAATGGGCAG TGCCCGGCGG CCAGAGAAAA GGGGCGCAGC CTCAGTCAAG 
ACCAGCGGGA GCCCCCGTTC CCCTGCAGGC CCCGAGAAGC CACGTGGCAC ACAGAAGACC 
ACGCCCGGGG TGCCAGCTGT GCTCCGGGGA CGAACAGTGA TCTACGTCCC CAGCCCGGCA 
CCCCGTGCCC AGCCCAAAGG GACCCCCGGC CCCCGCGCCA CACCGCGGAA GGTGGCGCCC 
CCTTGCCTGG CACAGCCCGC GGCTCCAGCC AAAGTCCCGA GCCCCGGGCA GCAGCGGTCG 
CGGAGCCTAC ACCGGCCTGC CAAGACCTCG GAGCTGGCGA CGCTGAGCCA GCCCCCCAGA 
AGCGCCACAC CGCCCGCCCG CCTCGCCAAG ACCCCCTCCT CCAGCTCCTC CCAGACCTCG 
CCCGCCTCCC AGCCCCTGCC CAGAAAGCGC CCCCCGGTCA CCCAGGCTGC TGGGGCCCTG 
CCCGGCCCCG GAGCCTCCCC GGTGCCCAAA ACGCCGGCGC GCACCCTTCT GGCGAAGCAG 
CACAAGACGC AGAGATCGCC CGTGCGGATC CCGTTCATGC AGAGGCCGGC CCGGCGTGGG 
CCGCCACCGC TGGCTCGGGC AGTCCCGGAG CCGGGCCCCA GGGGCCGGGC GGGGACCGAG 
GCGGGCCCGG GGGCGCGCGG GGGCCGCCTG GGCCTGGTGC GTGTGGCCTC AGCCCTCTCC 
AGCGGCAGCG AGTCCTCCGA CCGCTCGGGC TTCCGGCGAC AGCTAACCTT CATCAAGGAG 
TCGCCGGGCT TGCGGCGCCG CCGCTCCGAG CTGTCCTCGG CCGAGTCCGC GGCCTCTGCC 
CCCCAGGGCG CCTCGCCCCG CCGCGGCCGG CCCGCGCTGC CCGCCGTCTT CCTCTGCTCC 
TCGCGCTGCG AAGAGCTCCG AGCGGCACCC CGGCAGGGCC CGGCCCCGGC CCGGCAGCGG 
CCCCCCGCGG CCCGACCCAG CCCTGGCGAG CGCCCTGCCC GGCGCACCAC CTCCGAGAGC 
CCGTCCCGCC TGCCTGTGCG CGCGCCCGCC GCCCGGCCGG AGACTGTCAA GCGCTACGCG 
TCGCTGCCGC ACATCAGCGT GGCCCGCAGG CCCGACGGCG CCGTCCCCGC GGCCCCTGCC 
TCAGCCGACG CCGCGCGCCG CAGCAGCGAC GGGGAGCCCC GGCCGCTCCC CAGGGTGGCC 
GCGCCGGGCA CGACCTGGCG GCGCATCCGA GATGAGGACG TGCCCCACAT CCTGCGCAGC 
ACGCTTCCCG CCACGGCCCT GCCACTGCGG GGCTCCACGC CCGAGGACGC CCCGGCCGGG 
CCCCCGCCGC GCAAGACCAG CGACGCCGTG GTCCAGACCG AGGAGGTCGC CGCCCCCAAG 
ACCAACTCCA GCACGTCCCC GAGCCTGGAG ACCAGGGAGC CCCCCGGGGC CCCCGCCGGC 
GGCCAGCTCT CCCTCCTCGG CAGCGACGTG GACGGTCCCA GCCTCGCCAA GGCTCCCATC 
TCCGCACCCT TCGTGCACGA GGGCCTGGGG GTCGCCGTGG GGGGCTTCCC CGCCAGCCGG 
CACGGCTCCC CCAGCCGCTC GGCCCGAGTA CCCCCCTTCA ACTATGTGCC CAGCCCCATG 
GTGGTCGCAG CCACCACCGA CTCGGCCGCG GAGAAAGCCC CGGCCACTGC CTCCGCCACC 
CTCCTGGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APC2 ( XM_005259475.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259532.1 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X3, mRNA.

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CloneID	OHu51392
Clone ID Related Accession (Same CDS sequence)	XM_005259475.2
Accession Version	XM_005259475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005259475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGGGGCTCCT GAGCCTGCTG CACTCGGCCT TCTTCGGGGA CCAGACGCTG 
CAGGAGCTGA AGATGGCGAG CTCCGTGGCG CCCTACGAGC AGCTGGTGAG GCAGGTGGAG 
GCCTTGAAGG CTGAGAACAG CCACCTGAGG CAGGAGCTAA GGGACAACTC CAGCCACCTG 
TCCAAGCTGG AGACAGAGAC GTCGGGCATG AAGGAGGTCC TGAAGCACCT ACAGGGAAAA 
CTGGAGCAGG AGGCCCGAGT GCTGGTGTCC TCGGGGCAGA CGGAGGTGCT GGAGCAGCTG 
AAGGCCCTAC AGATGGACAT CACCAGCCTG TACAACCTCA AGTTCCAGCC GCCCACCCTG 
GGCCCGGAGC CTGCCGCCCG GACCCCCGAG GGCAGCCCAG TACACGGCTC CGGGCCCTCC 
AAGGACAGCT TTGGGGAGCT GAGCCGGGCC ACCATCCGGC TGCTGGAGGA ACTGGACCGG 
GAACGGTGTT TCCTGCTGAA TGAGATTGAG AAGGAGGAGA AGGAGAAGCT CTGGTACTAC 
TCTCAGCTGC AGGGCCTGTC CAAGCGCCTG GACGAGCTGC CGCACGTGGA GACGCAGTTC 
TCGATGCAGA TGGACCTGAT CCGGCAGCAG CTTGAGTTCG AGGCCCAGCA CATCCGCTCG 
CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCACA GATCCGCGCC 
TCGCGCCTGG AGCAGATTGA CAAGGAGCTG CTGGAGGCGC AGGACCGAGT GCAGCAGACG 
GAGCCCCAGG CCTTGCTGGC GGTGAAGTCG GTGCCGGTGG ACGAGGACCC CGAGACAGAG 
GTCCCCACAC ACCCTGAGGA TGGCACCCCT CAGCCGGGCA ACAGCAAGGT GGAGGTGGTC 
TTCTGGCTGT TGTCCATGTT GGCGACGCGC GACCAGGAGG ATACAGCGCG CACGCTGCTG 
GCCATGTCCA GCTCGCCCGA GAGCTGCGTG GCCATGCGCC GCTCGGGCTG TCTGCCTCTG 
CTGCTGCAAA TCCTCCACGG CACCGAGGCC GCGGCCGGGG GTCGCGCCGG GGCCCCAGGG 
GCACCGGGCG CCAAGGACGC ACGCATGCGC GCCAACGCGG CGCTGCACAA CATCGTCTTC 
TCGCAGCCGG ACCAGGGCCT GGCGCGCAAG GAGATGCGCG TCCTGCACGT GCTGGAGCAG 
ATCCGGGCCT ACTGCGAGAC CTGCTGGGAC TGGCTGCAGG CCCGAGACGG CGGGCCCGAG 
GGAGGTGGCG CCGGCAGCGC CCCGATCCCC ATCGAGCCGC AGATCTGCCA GGCCACCTGT 
GCTGTTATGA AGCTGTCCTT TGATGAGGAG TACCGCCGTG CCATGAACGA GCTAGGTGGG 
CTGCAGGCCG TGGCAGAGCT GCTGCAGGTT GACTATGAGA TGCACAAGAT GACCCGGGAC 
CCGCTGAACC TGGCGCTGCG CCGCTACGCG GGCATGACCC TCACCAACCT CACCTTTGGG 
GACGTTGCCA ACAAGGCCAC CCTGTGTGCG CGCCGCGGCT GCATGGAGGC CATCGTGGCC 
CAGCTGGCCT CCGACAGTGA GGAGCTCCAC CAGGTGGTGT CCAGCATCCT TCGGAACTTG 
TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AGGCGGGCAG CGTGACTGCC 
CTGGTGCAGT GTGTCCTGCG GGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTGAGCGCC 
CTGTGGAATC TGTCTGCACA CAGCACAGAG AACAAGGCGG CCATCTGCCA GGTGGATGGC 
GCCCTGGGCT TCCTGGTGAG CACCCTGACC TACAAGTGTC AGAGCAACTC GCTGGCCATC 
ATCGAGAGCG GCGGCGGCAT CCTCCGCAAT GTGTCCAGCC TCGTCGCCAC CCGTGAGGAC 
TACAGGCAGG TGCTCCGGGA TCACAACTGT CTGCAGACGC TGCTGCAGCA TCTGACTTCG 
CACAGCCTGA CCATCGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC 
GCCCGTGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG TAATCTGGTG 
CACTCCAAGC ACAAGATGAT CGCCATGGGC AGCGCCGCCG CCCTGCGCAA CCTGCTGGCC 
CATCGGCCCG CCAAGCACCA GGCGGCCGCC ACCGCCGTGT CCCCAGGCAG CTGCGTGCCC 
AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACGCACG GCACCTCGCG 
CAGGCGCTGG AGCACCTGGA GAAGCAGGGC CCGCCGGCAG CCGAGGCCGC CACTAAGAAG 
CCGCTGCCGC CCCTGCGACA CCTGGACGGC CTGGCCCAAG ACTATGCTTC CGATTCGGGC 
TGCTTTGACG ACGACGATGC ACCGTCATCC CTGGCTGCGG CCGCGGCCAC CGGGGAGCCA 
GCCAGCCCTG CCGCGCTGTC CCTCTTCCTG GGCAGCCCCT TCCTGCAGGG GCAGGCGCTG 
GCTCGCACCC CGCCCACCCG CCGAGGCGGC AAGGAGGCAG AGAAGGACAC CAGTGGGGAG 
GCAGCCGTGG CGGCCAAGGC CAAGGCCAAG CTGGCGCTTG CAGTGGCGCG CATCGACCAG 
CTGGTGGAGG ACATCTCCGC CCTGCACACC TCGTCCGACG ATAGCTTCAG CCTCAGCTCT 
GGAGACCCGG GACAGGAGGC GCCACGGGAG GGCCGCGCCC AGTCCTGCTC GCCATGCCGC 
GGCCCGGAGG GCGGGCGGCG AGAGGCAGGA AGCCGGGCGC ACCCGCTGCT GCGGCTCAAG 
GCGGCCCACG CCAGCCTCTC CAACGACAGC CTCAACAGCG GCAGTGCCAG CGACGGGTAC 
TGCCCACGCG AACATATGCT GCCCTGCCCG CTGGCCGCAC TGGCTTCGCG CCGCGAGGAC 
CCCAGGTGTG GGCAGCCTCG GCCCAGCCGG CTTGACCTTG ACCTGCCCGG CTGCCAGGCC 
GAGCCCCCGG CCCGCGAGGC CACCTCCGCC GACGCCCGCG TGCGCACCAT CAAGCTGTCG 
CCTACCTATC AGCACGTGCC ACTGCTTGAG GGTGCCTCAA GGGCGGGTGC AGAGCCCCTC 
GCGGGGCCTG GAATCTCTCC AGGGGCCCGG AAGCAGGCCT GGCTGCCGGC AGACCACCTG 
AGCAAGGTTC CCGAGAAGCT GGCGGCTGCC CCGCTGTCTG TGGCCAGCAA GGCACTGCAG 
AAACTGGCGG CGCAAGAGGG GCCACTCTCG CTGTCCCGAT GCAGCTCCCT TTCCTCGCTG 
TCCTCGGCCG GCCGCCCAGG CCCCAGCGAG GGTGGTGACC TGGATGACAG TGACTCCTCC 
CTGGAGGGGC TGGAGGAGGC CGGCCCCAGC GAGGCTGAGC TGGACAGCAC GTGGCGGGCG 
CCCGGGGCCA CCTCGCTGCC CGTAGCCATT CCGGCTCCCC GGCGTAACCG AGGCCGGGGC 
CTGGGGGTGG AAGACGCCAC GCCGTCCAGC TCGTCGGAGA ACTACGTGCA GGAGACACCG 
CTTGTGCTGA GCCGCTGCAG CTCTGTGAGC TCGCTGGGCA GCTTCGAGAG CCCGTCCATC 
GCCAGCTCCA TCCCCAGTGA ACCTTGCAGC GGGCAGGGCA GCGGCACCAT CAGCCCTAGC 
GAGCTGCCCG ACAGCCCCGG ACAGACCATG CCTCCCAGCC GGAGCAAGAC GCCACCGCTG 
GCGCCCGCGC CACAGGGTCC CCCCGAGGCC ACCCAGTTCA GCCTGCAGTG GGAGAGCTAC 
GTGAAGCGCT TCCTGGACAT CGCCGACTGC CGGGAGCGCT GCCGGCTGCC ATCTGAGCTG 
GACGCAGGCA GCGTGCGCTT TACCGTGGAG AAGCCAGACG AGAACTTCTC GTGCGCCTCC 
AGCCTCAGCG CGCTGGCCTT GCACGAGCAC TACGTGCAGC AGGACGTGGA GCTGCGGCTG 
CTGCCCTCGG CCTGCCCCGA GCGCGGCGGG GGCGCCGGGG GCGCCGGCCT CCACTTTGCA 
GGGCACCGGC GGCGGGAGGA GGGGCCGGCG CCCACGGGTT CTCGCCCTCG CGGCGCCGCG 
GACCAGGAGC TGGAACTGCT GCGGGAGTGC CTGGGAGCCG CCGTGCCTGC CCGGCTGCGC 
AAGGTGGCCT CCGCGCTGGT GCCAGGTCGC CGCGCACTCC CCGTGCCCGT CTACATGTTG 
GTGCCCGCCC CGGCCCCGGC CCAGGAGGAC GACTCCTGCA CTGACTCCGC GGAGGGCACG 
CCGGTCAACT TCTCTAGCGC CGCCTCGCTC AGCGACGAGA CGCTGCAGGG ACCCCCCAGG 
GACCAGCCCG GGGGACCAGC GGGCAGGCAA AGACCCACCG GCCGCCCCAC CTCTGCCAGA 
CAGGCCATGG GGCACCGGCA CAAGGCGGGA GGCGCCGGCC GCAGCGCGGA GCAGTCTCGG 
GGCGCGGGCA AGAACAGAGC AGGGCTGGAG CTGCCCCTGG GCCGGCCCCC GAGCGCCCCC 
GCAGACAAGG ACGGCTCAAA GCCCGGCCGG ACCCGCGGGG ACGGGGCGCT CCAGTCGCTG 
TGCCTCACGA CGCCCACTGA GGAGGCCGTG TACTGCTTCT ACGGCAACGA CTCGGACGAG 
GAGCCCCCGG CGGCCGCGCC CACGCCAACC CACCGGCGCA CATCGGCCAT CCCTCGCGCT 
TTTACGCGGG AGCGTCCGCA GGGCCGGAAG GAGGCCCCTG CCCCGTCCAA GGCTGCACCA 
GCTGCCCCGC CGCCCGCCCG GACCCAGCCC AGCCTCATTG CTGACGAGAC CCCGCCCTGC 
TACTCCCTGA GCTCCTCCGC CAGCTCCCTC AGCGAGCCCG AGCCCTCGGA GCCGCCGGCC 
GTCCATCCAC GAGGCCGGGA GCCCGCGGTC ACCAAGGACC CGGGCCCAGG AGGCGGACGC 
GACAGCTCGC CCAGCCCGCG GGCCGCGGAG GAGCTTCTGC AGCGGTGCAT CAGCTCGGCC 
CTGCCCAGGC GCCGGCCCCC CGTGTCTGGC CTGCGGCGCC GCAAGCCCCG AGCCACCCGG 
CTGGATGAGC GGCCCGCAGA GGGGTCCCGG GAACGCGGCG AGGAGGCAGC GGGCTCGGAC 
CGGGCCTCCG ACCTGGATAG CGTGGAGTGG CGCGCCATCC AGGAGGGCGC CAATTCAATT 
GTCACGTGGC TGCACCAGGC AGCAGCTGCC ACGCGGGAGG CCTCGTCCGA GTCCGACTCC 
ATCCTGTCCT TCGTATCCGG GCTGTCAGTG GGATCCACCC TACAGCCCCC CAAGCACAGG 
AAGGGACGAC AGGCGGAGGG AGAAATGGGC AGTGCCCGGC GGCCAGAGAA AAGGGGCGCA 
GCCTCAGTCA AGACCAGCGG GAGCCCCCGT TCCCCTGCAG GCCCCGAGAA GCCACGTGGC 
ACACAGAAGA CCACGCCCGG GGTGCCAGCT GTGCTCCGGG GACGAACAGT GATCTACGTC 
CCCAGCCCGG CACCCCGTGC CCAGCCCAAA GGGACCCCCG GCCCCCGCGC CACACCGCGG 
AAGGTGGCGC CCCCTTGCCT GGCACAGCCC GCGGCTCCAG CCAAAGTCCC GAGCCCCGGG 
CAGCAGCGGT CGCGGAGCCT ACACCGGCCT GCCAAGACCT CGGAGCTGGC GACGCTGAGC 
CAGCCCCCCA GAAGCGCCAC ACCGCCCGCC CGCCTCGCCA AGACCCCCTC CTCCAGCTCC 
TCCCAGACCT CGCCCGCCTC CCAGCCCCTG CCCAGAAAGC GCCCCCCGGT CACCCAGGCT 
GCTGGGGCCC TGCCCGGCCC CGGAGCCTCC CCGGTGCCCA AAACGCCGGC GCGCACCCTT 
CTGGCGAAGC AGCACAAGAC GCAGAGATCG CCCGTGCGGA TCCCGTTCAT GCAGAGGCCG 
GCCCGGCGTG GGCCGCCACC GCTGGCTCGG GCAGTCCCGG AGCCGGGCCC CAGGGGCCGG 
GCGGGGACCG AGGCGGGCCC GGGGGCGCGC GGGGGCCGCC TGGGCCTGGT GCGTGTGGCC 
TCAGCCCTCT CCAGCGGCAG CGAGTCCTCC GACCGCTCGG GCTTCCGGCG ACAGCTAACC 
TTCATCAAGG AGTCGCCGGG CTTGCGGCGC CGCCGCTCCG AGCTGTCCTC GGCCGAGTCC 
GCGGCCTCTG CCCCCCAGGG CGCCTCGCCC CGCCGCGGCC GGCCCGCGCT GCCCGCCGTC 
TTCCTCTGCT CCTCGCGCTG CGAAGAGCTC CGAGCGGCAC CCCGGCAGGG CCCGGCCCCG 
GCCCGGCAGC GGCCCCCCGC GGCCCGACCC AGCCCTGGCG AGCGCCCTGC CCGGCGCACC 
ACCTCCGAGA GCCCGTCCCG CCTGCCTGTG CGCGCGCCCG CCGCCCGGCC GGAGACTGTC 
AAGCGCTACG CGTCGCTGCC GCACATCAGC GTGGCCCGCA GGCCCGACGG CGCCGTCCCC 
GCGGCCCCTG CCTCAGCCGA CGCCGCGCGC CGCAGCAGCG ACGGGGAGCC CCGGCCGCTC 
CCCAGGGTGG CCGCGCCGGG CACGACCTGG CGGCGCATCC GAGATGAGGA CGTGCCCCAC 
ATCCTGCGCA GCACGCTTCC CGCCACGGCC CTGCCACTGC GGGGCTCCAC GCCCGAGGAC 
GCCCCGGCCG GGCCCCCGCC GCGCAAGACC AGCGACGCCG TGGTCCAGAC CGAGGAGGTC 
GCCGCCCCCA AGACCAACTC CAGCACGTCC CCGAGCCTGG AGACCAGGGA GCCCCCCGGG 
GCCCCCGCCG GCGGCCAGCT CTCCCTCCTC GGCAGCGACG TGGACGGTCC CAGCCTCGCC 
AAGGCTCCCA TCTCCGCACC CTTCGTGCAC GAGGGCCTGG GGGTCGCCGT GGGGGGCTTC 
CCCGCCAGCC GGCACGGCTC CCCCAGCCGC TCGGCCCGAG TACCCCCCTT CAACTATGTG 
CCCAGCCCCA TGGTGGTCGC AGCCACCACC GACTCGGCCG CGGAGAAAGC CCCGGCCACT 
GCCTCCGCCA CCCTCCTGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259532.1
CDS	21..7004
Translation	MGLLGLLSLLHSAFFGDQTLQELKMASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETQFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	XM_005259475.2
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259475.2

ORF Insert Sequence:

ATGGGGCTCC TGGGGCTCCT GAGCCTGCTG CACTCGGCCT TCTTCGGGGA CCAGACGCTG 
CAGGAGCTGA AGATGGCGAG CTCCGTGGCG CCCTACGAGC AGCTGGTGAG GCAGGTGGAG 
GCCTTGAAGG CTGAGAACAG CCACCTGAGG CAGGAGCTAA GGGACAACTC CAGCCACCTG 
TCCAAGCTGG AGACAGAGAC GTCGGGCATG AAGGAGGTCC TGAAGCACCT ACAGGGAAAA 
CTGGAGCAGG AGGCCCGAGT GCTGGTGTCC TCGGGGCAGA CGGAGGTGCT GGAGCAGCTG 
AAGGCCCTAC AGATGGACAT CACCAGCCTG TACAACCTCA AGTTCCAGCC GCCCACCCTG 
GGCCCGGAGC CTGCCGCCCG GACCCCCGAG GGCAGCCCAG TACACGGCTC CGGGCCCTCC 
AAGGACAGCT TTGGGGAGCT GAGCCGGGCC ACCATCCGGC TGCTGGAGGA ACTGGACCGG 
GAACGGTGTT TCCTGCTGAA TGAGATTGAG AAGGAGGAGA AGGAGAAGCT CTGGTACTAC 
TCTCAGCTGC AGGGCCTGTC CAAGCGCCTG GACGAGCTGC CGCACGTGGA GACGCAGTTC 
TCGATGCAGA TGGACCTGAT CCGGCAGCAG CTTGAGTTCG AGGCCCAGCA CATCCGCTCG 
CTGATGGAGG AGCGCTTCGG CACCTCGGAC GAGATGGTGC AGCGGGCACA GATCCGCGCC 
TCGCGCCTGG AGCAGATTGA CAAGGAGCTG CTGGAGGCGC AGGACCGAGT GCAGCAGACG 
GAGCCCCAGG CCTTGCTGGC GGTGAAGTCG GTGCCGGTGG ACGAGGACCC CGAGACAGAG 
GTCCCCACAC ACCCTGAGGA TGGCACCCCT CAGCCGGGCA ACAGCAAGGT GGAGGTGGTC 
TTCTGGCTGT TGTCCATGTT GGCGACGCGC GACCAGGAGG ATACAGCGCG CACGCTGCTG 
GCCATGTCCA GCTCGCCCGA GAGCTGCGTG GCCATGCGCC GCTCGGGCTG TCTGCCTCTG 
CTGCTGCAAA TCCTCCACGG CACCGAGGCC GCGGCCGGGG GTCGCGCCGG GGCCCCAGGG 
GCACCGGGCG CCAAGGACGC ACGCATGCGC GCCAACGCGG CGCTGCACAA CATCGTCTTC 
TCGCAGCCGG ACCAGGGCCT GGCGCGCAAG GAGATGCGCG TCCTGCACGT GCTGGAGCAG 
ATCCGGGCCT ACTGCGAGAC CTGCTGGGAC TGGCTGCAGG CCCGAGACGG CGGGCCCGAG 
GGAGGTGGCG CCGGCAGCGC CCCGATCCCC ATCGAGCCGC AGATCTGCCA GGCCACCTGT 
GCTGTTATGA AGCTGTCCTT TGATGAGGAG TACCGCCGTG CCATGAACGA GCTAGGTGGG 
CTGCAGGCCG TGGCAGAGCT GCTGCAGGTT GACTATGAGA TGCACAAGAT GACCCGGGAC 
CCGCTGAACC TGGCGCTGCG CCGCTACGCG GGCATGACCC TCACCAACCT CACCTTTGGG 
GACGTTGCCA ACAAGGCCAC CCTGTGTGCG CGCCGCGGCT GCATGGAGGC CATCGTGGCC 
CAGCTGGCCT CCGACAGTGA GGAGCTCCAC CAGGTGGTGT CCAGCATCCT TCGGAACTTG 
TCCTGGAGGG CCGACATCAA CAGCAAGAAG GTGCTGAGGG AGGCGGGCAG CGTGACTGCC 
CTGGTGCAGT GTGTCCTGCG GGCCACCAAG GAGTCCACCC TGAAGAGCGT GCTGAGCGCC 
CTGTGGAATC TGTCTGCACA CAGCACAGAG AACAAGGCGG CCATCTGCCA GGTGGATGGC 
GCCCTGGGCT TCCTGGTGAG CACCCTGACC TACAAGTGTC AGAGCAACTC GCTGGCCATC 
ATCGAGAGCG GCGGCGGCAT CCTCCGCAAT GTGTCCAGCC TCGTCGCCAC CCGTGAGGAC 
TACAGGCAGG TGCTCCGGGA TCACAACTGT CTGCAGACGC TGCTGCAGCA TCTGACTTCG 
CACAGCCTGA CCATCGTGAG CAACGCGTGC GGCACGCTCT GGAACCTGTC GGCCCGCAGC 
GCCCGTGACC AGGAGCTGCT GTGGGACCTG GGCGCCGTGG GCATGCTGCG TAATCTGGTG 
CACTCCAAGC ACAAGATGAT CGCCATGGGC AGCGCCGCCG CCCTGCGCAA CCTGCTGGCC 
CATCGGCCCG CCAAGCACCA GGCGGCCGCC ACCGCCGTGT CCCCAGGCAG CTGCGTGCCC 
AGCCTGTACG TGCGCAAGCA GCGGGCGCTG GAGGCCGAGC TGGACGCACG GCACCTCGCG 
CAGGCGCTGG AGCACCTGGA GAAGCAGGGC CCGCCGGCAG CCGAGGCCGC CACTAAGAAG 
CCGCTGCCGC CCCTGCGACA CCTGGACGGC CTGGCCCAAG ACTATGCTTC CGATTCGGGC 
TGCTTTGACG ACGACGATGC ACCGTCATCC CTGGCTGCGG CCGCGGCCAC CGGGGAGCCA 
GCCAGCCCTG CCGCGCTGTC CCTCTTCCTG GGCAGCCCCT TCCTGCAGGG GCAGGCGCTG 
GCTCGCACCC CGCCCACCCG CCGAGGCGGC AAGGAGGCAG AGAAGGACAC CAGTGGGGAG 
GCAGCCGTGG CGGCCAAGGC CAAGGCCAAG CTGGCGCTTG CAGTGGCGCG CATCGACCAG 
CTGGTGGAGG ACATCTCCGC CCTGCACACC TCGTCCGACG ATAGCTTCAG CCTCAGCTCT 
GGAGACCCGG GACAGGAGGC GCCACGGGAG GGCCGCGCCC AGTCCTGCTC GCCATGCCGC 
GGCCCGGAGG GCGGGCGGCG AGAGGCAGGA AGCCGGGCGC ACCCGCTGCT GCGGCTCAAG 
GCGGCCCACG CCAGCCTCTC CAACGACAGC CTCAACAGCG GCAGTGCCAG CGACGGGTAC 
TGCCCACGCG AACATATGCT GCCCTGCCCG CTGGCCGCAC TGGCTTCGCG CCGCGAGGAC 
CCCAGGTGTG GGCAGCCTCG GCCCAGCCGG CTTGACCTTG ACCTGCCCGG CTGCCAGGCC 
GAGCCCCCGG CCCGCGAGGC CACCTCCGCC GACGCCCGCG TGCGCACCAT CAAGCTGTCG 
CCTACCTATC AGCACGTGCC ACTGCTTGAG GGTGCCTCAA GGGCGGGTGC AGAGCCCCTC 
GCGGGGCCTG GAATCTCTCC AGGGGCCCGG AAGCAGGCCT GGCTGCCGGC AGACCACCTG 
AGCAAGGTTC CCGAGAAGCT GGCGGCTGCC CCGCTGTCTG TGGCCAGCAA GGCACTGCAG 
AAACTGGCGG CGCAAGAGGG GCCACTCTCG CTGTCCCGAT GCAGCTCCCT TTCCTCGCTG 
TCCTCGGCCG GCCGCCCAGG CCCCAGCGAG GGTGGTGACC TGGATGACAG TGACTCCTCC 
CTGGAGGGGC TGGAGGAGGC CGGCCCCAGC GAGGCTGAGC TGGACAGCAC GTGGCGGGCG 
CCCGGGGCCA CCTCGCTGCC CGTAGCCATT CCGGCTCCCC GGCGTAACCG AGGCCGGGGC 
CTGGGGGTGG AAGACGCCAC GCCGTCCAGC TCGTCGGAGA ACTACGTGCA GGAGACACCG 
CTTGTGCTGA GCCGCTGCAG CTCTGTGAGC TCGCTGGGCA GCTTCGAGAG CCCGTCCATC 
GCCAGCTCCA TCCCCAGTGA ACCTTGCAGC GGGCAGGGCA GCGGCACCAT CAGCCCTAGC 
GAGCTGCCCG ACAGCCCCGG ACAGACCATG CCTCCCAGCC GGAGCAAGAC GCCACCGCTG 
GCGCCCGCGC CACAGGGTCC CCCCGAGGCC ACCCAGTTCA GCCTGCAGTG GGAGAGCTAC 
GTGAAGCGCT TCCTGGACAT CGCCGACTGC CGGGAGCGCT GCCGGCTGCC ATCTGAGCTG 
GACGCAGGCA GCGTGCGCTT TACCGTGGAG AAGCCAGACG AGAACTTCTC GTGCGCCTCC 
AGCCTCAGCG CGCTGGCCTT GCACGAGCAC TACGTGCAGC AGGACGTGGA GCTGCGGCTG 
CTGCCCTCGG CCTGCCCCGA GCGCGGCGGG GGCGCCGGGG GCGCCGGCCT CCACTTTGCA 
GGGCACCGGC GGCGGGAGGA GGGGCCGGCG CCCACGGGTT CTCGCCCTCG CGGCGCCGCG 
GACCAGGAGC TGGAACTGCT GCGGGAGTGC CTGGGAGCCG CCGTGCCTGC CCGGCTGCGC 
AAGGTGGCCT CCGCGCTGGT GCCAGGTCGC CGCGCACTCC CCGTGCCCGT CTACATGTTG 
GTGCCCGCCC CGGCCCCGGC CCAGGAGGAC GACTCCTGCA CTGACTCCGC GGAGGGCACG 
CCGGTCAACT TCTCTAGCGC CGCCTCGCTC AGCGACGAGA CGCTGCAGGG ACCCCCCAGG 
GACCAGCCCG GGGGACCAGC GGGCAGGCAA AGACCCACCG GCCGCCCCAC CTCTGCCAGA 
CAGGCCATGG GGCACCGGCA CAAGGCGGGA GGCGCCGGCC GCAGCGCGGA GCAGTCTCGG 
GGCGCGGGCA AGAACAGAGC AGGGCTGGAG CTGCCCCTGG GCCGGCCCCC GAGCGCCCCC 
GCAGACAAGG ACGGCTCAAA GCCCGGCCGG ACCCGCGGGG ACGGGGCGCT CCAGTCGCTG 
TGCCTCACGA CGCCCACTGA GGAGGCCGTG TACTGCTTCT ACGGCAACGA CTCGGACGAG 
GAGCCCCCGG CGGCCGCGCC CACGCCAACC CACCGGCGCA CATCGGCCAT CCCTCGCGCT 
TTTACGCGGG AGCGTCCGCA GGGCCGGAAG GAGGCCCCTG CCCCGTCCAA GGCTGCACCA 
GCTGCCCCGC CGCCCGCCCG GACCCAGCCC AGCCTCATTG CTGACGAGAC CCCGCCCTGC 
TACTCCCTGA GCTCCTCCGC CAGCTCCCTC AGCGAGCCCG AGCCCTCGGA GCCGCCGGCC 
GTCCATCCAC GAGGCCGGGA GCCCGCGGTC ACCAAGGACC CGGGCCCAGG AGGCGGACGC 
GACAGCTCGC CCAGCCCGCG GGCCGCGGAG GAGCTTCTGC AGCGGTGCAT CAGCTCGGCC 
CTGCCCAGGC GCCGGCCCCC CGTGTCTGGC CTGCGGCGCC GCAAGCCCCG AGCCACCCGG 
CTGGATGAGC GGCCCGCAGA GGGGTCCCGG GAACGCGGCG AGGAGGCAGC GGGCTCGGAC 
CGGGCCTCCG ACCTGGATAG CGTGGAGTGG CGCGCCATCC AGGAGGGCGC CAATTCAATT 
GTCACGTGGC TGCACCAGGC AGCAGCTGCC ACGCGGGAGG CCTCGTCCGA GTCCGACTCC 
ATCCTGTCCT TCGTATCCGG GCTGTCAGTG GGATCCACCC TACAGCCCCC CAAGCACAGG 
AAGGGACGAC AGGCGGAGGG AGAAATGGGC AGTGCCCGGC GGCCAGAGAA AAGGGGCGCA 
GCCTCAGTCA AGACCAGCGG GAGCCCCCGT TCCCCTGCAG GCCCCGAGAA GCCACGTGGC 
ACACAGAAGA CCACGCCCGG GGTGCCAGCT GTGCTCCGGG GACGAACAGT GATCTACGTC 
CCCAGCCCGG CACCCCGTGC CCAGCCCAAA GGGACCCCCG GCCCCCGCGC CACACCGCGG 
AAGGTGGCGC CCCCTTGCCT GGCACAGCCC GCGGCTCCAG CCAAAGTCCC GAGCCCCGGG 
CAGCAGCGGT CGCGGAGCCT ACACCGGCCT GCCAAGACCT CGGAGCTGGC GACGCTGAGC 
CAGCCCCCCA GAAGCGCCAC ACCGCCCGCC CGCCTCGCCA AGACCCCCTC CTCCAGCTCC 
TCCCAGACCT CGCCCGCCTC CCAGCCCCTG CCCAGAAAGC GCCCCCCGGT CACCCAGGCT 
GCTGGGGCCC TGCCCGGCCC CGGAGCCTCC CCGGTGCCCA AAACGCCGGC GCGCACCCTT 
CTGGCGAAGC AGCACAAGAC GCAGAGATCG CCCGTGCGGA TCCCGTTCAT GCAGAGGCCG 
GCCCGGCGTG GGCCGCCACC GCTGGCTCGG GCAGTCCCGG AGCCGGGCCC CAGGGGCCGG 
GCGGGGACCG AGGCGGGCCC GGGGGCGCGC GGGGGCCGCC TGGGCCTGGT GCGTGTGGCC 
TCAGCCCTCT CCAGCGGCAG CGAGTCCTCC GACCGCTCGG GCTTCCGGCG ACAGCTAACC 
TTCATCAAGG AGTCGCCGGG CTTGCGGCGC CGCCGCTCCG AGCTGTCCTC GGCCGAGTCC 
GCGGCCTCTG CCCCCCAGGG CGCCTCGCCC CGCCGCGGCC GGCCCGCGCT GCCCGCCGTC 
TTCCTCTGCT CCTCGCGCTG CGAAGAGCTC CGAGCGGCAC CCCGGCAGGG CCCGGCCCCG 
GCCCGGCAGC GGCCCCCCGC GGCCCGACCC AGCCCTGGCG AGCGCCCTGC CCGGCGCACC 
ACCTCCGAGA GCCCGTCCCG CCTGCCTGTG CGCGCGCCCG CCGCCCGGCC GGAGACTGTC 
AAGCGCTACG CGTCGCTGCC GCACATCAGC GTGGCCCGCA GGCCCGACGG CGCCGTCCCC 
GCGGCCCCTG CCTCAGCCGA CGCCGCGCGC CGCAGCAGCG ACGGGGAGCC CCGGCCGCTC 
CCCAGGGTGG CCGCGCCGGG CACGACCTGG CGGCGCATCC GAGATGAGGA CGTGCCCCAC 
ATCCTGCGCA GCACGCTTCC CGCCACGGCC CTGCCACTGC GGGGCTCCAC GCCCGAGGAC 
GCCCCGGCCG GGCCCCCGCC GCGCAAGACC AGCGACGCCG TGGTCCAGAC CGAGGAGGTC 
GCCGCCCCCA AGACCAACTC CAGCACGTCC CCGAGCCTGG AGACCAGGGA GCCCCCCGGG 
GCCCCCGCCG GCGGCCAGCT CTCCCTCCTC GGCAGCGACG TGGACGGTCC CAGCCTCGCC 
AAGGCTCCCA TCTCCGCACC CTTCGTGCAC GAGGGCCTGG GGGTCGCCGT GGGGGGCTTC 
CCCGCCAGCC GGCACGGCTC CCCCAGCCGC TCGGCCCGAG TACCCCCCTT CAACTATGTG 
CCCAGCCCCA TGGTGGTCGC AGCCACCACC GACTCGGCCG CGGAGAAAGC CCCGGCCACT 
GCCTCCGCCA CCCTCCTGGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APC2 ( XM_006722607.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722670.1 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X4, mRNA.

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CloneID	OHu51393
Clone ID Related Accession (Same CDS sequence)	XM_006722607.2
Accession Version	XM_006722607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006722607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGGGGCTCCT GAGCCTGCTG CACTCGGCCT TCTTCGGGGA CCAGACGCTG 
CAGGAGCTGA AGATGGCGAG CTCCGTGGCG CCCTACGAGC AGCTGGTGAG GCAGGTGGAG 
GCCTTGAAGG CTGAGAACAG CCACCTGAGG CAGGAGCTAA GGGACAACTC CAGCCACCTG 
TCCAAGCTGG AGACAGAGAC GTCGGGCATG AAGGAGGTCC TGAAGCACCT ACAGGGAAAA 
CTGGAGCAGG AGGCCCGAGT GCTGGTGTCC TCGGGGCAGA CGGAGGTGCT GGAGCAGCTG 
AAGGCCCTAC AGATGGACAT CACCAGCCTG TACAACCTCA AGTTCCAGCC GCCCACCCTG 
GGCCCGGAGC CTGCCGCCCG GACCCCCGAG GGCAGCCCAG TACACGGCTC CGGGCCCTCC 
AAGGACAGCT TTGGGGAGCT GAGCCGGGCC ACCATCCGGC TGCTGGAGGA ACTGGACCGG 
GAACGGTGTT TCCTGCTGAA TGAGATTGAG AAGGAGGAGA AGGAGAAGCT CTGGTACTAC 
TCTCAGCTGC AGGGCCTGTC CAAGCGCCTG GACGAGCTGC CGCACGTGGA GACGTTCTCG 
ATGCAGATGG ACCTGATCCG GCAGCAGCTT GAGTTCGAGG CCCAGCACAT CCGCTCGCTG 
ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCACAGAT CCGCGCCTCG 
CGCCTGGAGC AGATTGACAA GGAGCTGCTG GAGGCGCAGG ACCGAGTGCA GCAGACGGAG 
CCCCAGGCCT TGCTGGCGGT GAAGTCGGTG CCGGTGGACG AGGACCCCGA GACAGAGGTC 
CCCACACACC CTGAGGATGG CACCCCTCAG CCGGGCAACA GCAAGGTGGA GGTGGTCTTC 
TGGCTGTTGT CCATGTTGGC GACGCGCGAC CAGGAGGATA CAGCGCGCAC GCTGCTGGCC 
ATGTCCAGCT CGCCCGAGAG CTGCGTGGCC ATGCGCCGCT CGGGCTGTCT GCCTCTGCTG 
CTGCAAATCC TCCACGGCAC CGAGGCCGCG GCCGGGGGTC GCGCCGGGGC CCCAGGGGCA 
CCGGGCGCCA AGGACGCACG CATGCGCGCC AACGCGGCGC TGCACAACAT CGTCTTCTCG 
CAGCCGGACC AGGGCCTGGC GCGCAAGGAG ATGCGCGTCC TGCACGTGCT GGAGCAGATC 
CGGGCCTACT GCGAGACCTG CTGGGACTGG CTGCAGGCCC GAGACGGCGG GCCCGAGGGA 
GGTGGCGCCG GCAGCGCCCC GATCCCCATC GAGCCGCAGA TCTGCCAGGC CACCTGTGCT 
GTTATGAAGC TGTCCTTTGA TGAGGAGTAC CGCCGTGCCA TGAACGAGCT AGGTGGGCTG 
CAGGCCGTGG CAGAGCTGCT GCAGGTTGAC TATGAGATGC ACAAGATGAC CCGGGACCCG 
CTGAACCTGG CGCTGCGCCG CTACGCGGGC ATGACCCTCA CCAACCTCAC CTTTGGGGAC 
GTTGCCAACA AGGCCACCCT GTGTGCGCGC CGCGGCTGCA TGGAGGCCAT CGTGGCCCAG 
CTGGCCTCCG ACAGTGAGGA GCTCCACCAG GTGGTGTCCA GCATCCTTCG GAACTTGTCC 
TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAGG CGGGCAGCGT GACTGCCCTG 
GTGCAGTGTG TCCTGCGGGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT GAGCGCCCTG 
TGGAATCTGT CTGCACACAG CACAGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGCGCC 
CTGGGCTTCC TGGTGAGCAC CCTGACCTAC AAGTGTCAGA GCAACTCGCT GGCCATCATC 
GAGAGCGGCG GCGGCATCCT CCGCAATGTG TCCAGCCTCG TCGCCACCCG TGAGGACTAC 
AGGCAGGTGC TCCGGGATCA CAACTGTCTG CAGACGCTGC TGCAGCATCT GACTTCGCAC 
AGCCTGACCA TCGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCGCC 
CGTGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGTAA TCTGGTGCAC 
TCCAAGCACA AGATGATCGC CATGGGCAGC GCCGCCGCCC TGCGCAACCT GCTGGCCCAT 
CGGCCCGCCA AGCACCAGGC GGCCGCCACC GCCGTGTCCC CAGGCAGCTG CGTGCCCAGC 
CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACGCACGGCA CCTCGCGCAG 
GCGCTGGAGC ACCTGGAGAA GCAGGGCCCG CCGGCAGCCG AGGCCGCCAC TAAGAAGCCG 
CTGCCGCCCC TGCGACACCT GGACGGCCTG GCCCAAGACT ATGCTTCCGA TTCGGGCTGC 
TTTGACGACG ACGATGCACC GTCATCCCTG GCTGCGGCCG CGGCCACCGG GGAGCCAGCC 
AGCCCTGCCG CGCTGTCCCT CTTCCTGGGC AGCCCCTTCC TGCAGGGGCA GGCGCTGGCT 
CGCACCCCGC CCACCCGCCG AGGCGGCAAG GAGGCAGAGA AGGACACCAG TGGGGAGGCA 
GCCGTGGCGG CCAAGGCCAA GGCCAAGCTG GCGCTTGCAG TGGCGCGCAT CGACCAGCTG 
GTGGAGGACA TCTCCGCCCT GCACACCTCG TCCGACGATA GCTTCAGCCT CAGCTCTGGA 
GACCCGGGAC AGGAGGCGCC ACGGGAGGGC CGCGCCCAGT CCTGCTCGCC ATGCCGCGGC 
CCGGAGGGCG GGCGGCGAGA GGCAGGAAGC CGGGCGCACC CGCTGCTGCG GCTCAAGGCG 
GCCCACGCCA GCCTCTCCAA CGACAGCCTC AACAGCGGCA GTGCCAGCGA CGGGTACTGC 
CCACGCGAAC ATATGCTGCC CTGCCCGCTG GCCGCACTGG CTTCGCGCCG CGAGGACCCC 
AGGTGTGGGC AGCCTCGGCC CAGCCGGCTT GACCTTGACC TGCCCGGCTG CCAGGCCGAG 
CCCCCGGCCC GCGAGGCCAC CTCCGCCGAC GCCCGCGTGC GCACCATCAA GCTGTCGCCT 
ACCTATCAGC ACGTGCCACT GCTTGAGGGT GCCTCAAGGG CGGGTGCAGA GCCCCTCGCG 
GGGCCTGGAA TCTCTCCAGG GGCCCGGAAG CAGGCCTGGC TGCCGGCAGA CCACCTGAGC 
AAGGTTCCCG AGAAGCTGGC GGCTGCCCCG CTGTCTGTGG CCAGCAAGGC ACTGCAGAAA 
CTGGCGGCGC AAGAGGGGCC ACTCTCGCTG TCCCGATGCA GCTCCCTTTC CTCGCTGTCC 
TCGGCCGGCC GCCCAGGCCC CAGCGAGGGT GGTGACCTGG ATGACAGTGA CTCCTCCCTG 
GAGGGGCTGG AGGAGGCCGG CCCCAGCGAG GCTGAGCTGG ACAGCACGTG GCGGGCGCCC 
GGGGCCACCT CGCTGCCCGT AGCCATTCCG GCTCCCCGGC GTAACCGAGG CCGGGGCCTG 
GGGGTGGAAG ACGCCACGCC GTCCAGCTCG TCGGAGAACT ACGTGCAGGA GACACCGCTT 
GTGCTGAGCC GCTGCAGCTC TGTGAGCTCG CTGGGCAGCT TCGAGAGCCC GTCCATCGCC 
AGCTCCATCC CCAGTGAACC TTGCAGCGGG CAGGGCAGCG GCACCATCAG CCCTAGCGAG 
CTGCCCGACA GCCCCGGACA GACCATGCCT CCCAGCCGGA GCAAGACGCC ACCGCTGGCG 
CCCGCGCCAC AGGGTCCCCC CGAGGCCACC CAGTTCAGCC TGCAGTGGGA GAGCTACGTG 
AAGCGCTTCC TGGACATCGC CGACTGCCGG GAGCGCTGCC GGCTGCCATC TGAGCTGGAC 
GCAGGCAGCG TGCGCTTTAC CGTGGAGAAG CCAGACGAGA ACTTCTCGTG CGCCTCCAGC 
CTCAGCGCGC TGGCCTTGCA CGAGCACTAC GTGCAGCAGG ACGTGGAGCT GCGGCTGCTG 
CCCTCGGCCT GCCCCGAGCG CGGCGGGGGC GCCGGGGGCG CCGGCCTCCA CTTTGCAGGG 
CACCGGCGGC GGGAGGAGGG GCCGGCGCCC ACGGGTTCTC GCCCTCGCGG CGCCGCGGAC 
CAGGAGCTGG AACTGCTGCG GGAGTGCCTG GGAGCCGCCG TGCCTGCCCG GCTGCGCAAG 
GTGGCCTCCG CGCTGGTGCC AGGTCGCCGC GCACTCCCCG TGCCCGTCTA CATGTTGGTG 
CCCGCCCCGG CCCCGGCCCA GGAGGACGAC TCCTGCACTG ACTCCGCGGA GGGCACGCCG 
GTCAACTTCT CTAGCGCCGC CTCGCTCAGC GACGAGACGC TGCAGGGACC CCCCAGGGAC 
CAGCCCGGGG GACCAGCGGG CAGGCAAAGA CCCACCGGCC GCCCCACCTC TGCCAGACAG 
GCCATGGGGC ACCGGCACAA GGCGGGAGGC GCCGGCCGCA GCGCGGAGCA GTCTCGGGGC 
GCGGGCAAGA ACAGAGCAGG GCTGGAGCTG CCCCTGGGCC GGCCCCCGAG CGCCCCCGCA 
GACAAGGACG GCTCAAAGCC CGGCCGGACC CGCGGGGACG GGGCGCTCCA GTCGCTGTGC 
CTCACGACGC CCACTGAGGA GGCCGTGTAC TGCTTCTACG GCAACGACTC GGACGAGGAG 
CCCCCGGCGG CCGCGCCCAC GCCAACCCAC CGGCGCACAT CGGCCATCCC TCGCGCTTTT 
ACGCGGGAGC GTCCGCAGGG CCGGAAGGAG GCCCCTGCCC CGTCCAAGGC TGCACCAGCT 
GCCCCGCCGC CCGCCCGGAC CCAGCCCAGC CTCATTGCTG ACGAGACCCC GCCCTGCTAC 
TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC GAGCCCGAGC CCTCGGAGCC GCCGGCCGTC 
CATCCACGAG GCCGGGAGCC CGCGGTCACC AAGGACCCGG GCCCAGGAGG CGGACGCGAC 
AGCTCGCCCA GCCCGCGGGC CGCGGAGGAG CTTCTGCAGC GGTGCATCAG CTCGGCCCTG 
CCCAGGCGCC GGCCCCCCGT GTCTGGCCTG CGGCGCCGCA AGCCCCGAGC CACCCGGCTG 
GATGAGCGGC CCGCAGAGGG GTCCCGGGAA CGCGGCGAGG AGGCAGCGGG CTCGGACCGG 
GCCTCCGACC TGGATAGCGT GGAGTGGCGC GCCATCCAGG AGGGCGCCAA TTCAATTGTC 
ACGTGGCTGC ACCAGGCAGC AGCTGCCACG CGGGAGGCCT CGTCCGAGTC CGACTCCATC 
CTGTCCTTCG TATCCGGGCT GTCAGTGGGA TCCACCCTAC AGCCCCCCAA GCACAGGAAG 
GGACGACAGG CGGAGGGAGA AATGGGCAGT GCCCGGCGGC CAGAGAAAAG GGGCGCAGCC 
TCAGTCAAGA CCAGCGGGAG CCCCCGTTCC CCTGCAGGCC CCGAGAAGCC ACGTGGCACA 
CAGAAGACCA CGCCCGGGGT GCCAGCTGTG CTCCGGGGAC GAACAGTGAT CTACGTCCCC 
AGCCCGGCAC CCCGTGCCCA GCCCAAAGGG ACCCCCGGCC CCCGCGCCAC ACCGCGGAAG 
GTGGCGCCCC CTTGCCTGGC ACAGCCCGCG GCTCCAGCCA AAGTCCCGAG CCCCGGGCAG 
CAGCGGTCGC GGAGCCTACA CCGGCCTGCC AAGACCTCGG AGCTGGCGAC GCTGAGCCAG 
CCCCCCAGAA GCGCCACACC GCCCGCCCGC CTCGCCAAGA CCCCCTCCTC CAGCTCCTCC 
CAGACCTCGC CCGCCTCCCA GCCCCTGCCC AGAAAGCGCC CCCCGGTCAC CCAGGCTGCT 
GGGGCCCTGC CCGGCCCCGG AGCCTCCCCG GTGCCCAAAA CGCCGGCGCG CACCCTTCTG 
GCGAAGCAGC ACAAGACGCA GAGATCGCCC GTGCGGATCC CGTTCATGCA GAGGCCGGCC 
CGGCGTGGGC CGCCACCGCT GGCTCGGGCA GTCCCGGAGC CGGGCCCCAG GGGCCGGGCG 
GGGACCGAGG CGGGCCCGGG GGCGCGCGGG GGCCGCCTGG GCCTGGTGCG TGTGGCCTCA 
GCCCTCTCCA GCGGCAGCGA GTCCTCCGAC CGCTCGGGCT TCCGGCGACA GCTAACCTTC 
ATCAAGGAGT CGCCGGGCTT GCGGCGCCGC CGCTCCGAGC TGTCCTCGGC CGAGTCCGCG 
GCCTCTGCCC CCCAGGGCGC CTCGCCCCGC CGCGGCCGGC CCGCGCTGCC CGCCGTCTTC 
CTCTGCTCCT CGCGCTGCGA AGAGCTCCGA GCGGCACCCC GGCAGGGCCC GGCCCCGGCC 
CGGCAGCGGC CCCCCGCGGC CCGACCCAGC CCTGGCGAGC GCCCTGCCCG GCGCACCACC 
TCCGAGAGCC CGTCCCGCCT GCCTGTGCGC GCGCCCGCCG CCCGGCCGGA GACTGTCAAG 
CGCTACGCGT CGCTGCCGCA CATCAGCGTG GCCCGCAGGC CCGACGGCGC CGTCCCCGCG 
GCCCCTGCCT CAGCCGACGC CGCGCGCCGC AGCAGCGACG GGGAGCCCCG GCCGCTCCCC 
AGGGTGGCCG CGCCGGGCAC GACCTGGCGG CGCATCCGAG ATGAGGACGT GCCCCACATC 
CTGCGCAGCA CGCTTCCCGC CACGGCCCTG CCACTGCGGG GCTCCACGCC CGAGGACGCC 
CCGGCCGGGC CCCCGCCGCG CAAGACCAGC GACGCCGTGG TCCAGACCGA GGAGGTCGCC 
GCCCCCAAGA CCAACTCCAG CACGTCCCCG AGCCTGGAGA CCAGGGAGCC CCCCGGGGCC 
CCCGCCGGCG GCCAGCTCTC CCTCCTCGGC AGCGACGTGG ACGGTCCCAG CCTCGCCAAG 
GCTCCCATCT CCGCACCCTT CGTGCACGAG GGCCTGGGGG TCGCCGTGGG GGGCTTCCCC 
GCCAGCCGGC ACGGCTCCCC CAGCCGCTCG GCCCGAGTAC CCCCCTTCAA CTATGTGCCC 
AGCCCCATGG TGGTCGCAGC CACCACCGAC TCGGCCGCGG AGAAAGCCCC GGCCACTGCC 
TCCGCCACCC TCCTGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722670.1
CDS	21..7001
Translation	MGLLGLLSLLHSAFFGDQTLQELKMASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	XM_006722607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722607.2

ORF Insert Sequence:

ATGGGGCTCC TGGGGCTCCT GAGCCTGCTG CACTCGGCCT TCTTCGGGGA CCAGACGCTG 
CAGGAGCTGA AGATGGCGAG CTCCGTGGCG CCCTACGAGC AGCTGGTGAG GCAGGTGGAG 
GCCTTGAAGG CTGAGAACAG CCACCTGAGG CAGGAGCTAA GGGACAACTC CAGCCACCTG 
TCCAAGCTGG AGACAGAGAC GTCGGGCATG AAGGAGGTCC TGAAGCACCT ACAGGGAAAA 
CTGGAGCAGG AGGCCCGAGT GCTGGTGTCC TCGGGGCAGA CGGAGGTGCT GGAGCAGCTG 
AAGGCCCTAC AGATGGACAT CACCAGCCTG TACAACCTCA AGTTCCAGCC GCCCACCCTG 
GGCCCGGAGC CTGCCGCCCG GACCCCCGAG GGCAGCCCAG TACACGGCTC CGGGCCCTCC 
AAGGACAGCT TTGGGGAGCT GAGCCGGGCC ACCATCCGGC TGCTGGAGGA ACTGGACCGG 
GAACGGTGTT TCCTGCTGAA TGAGATTGAG AAGGAGGAGA AGGAGAAGCT CTGGTACTAC 
TCTCAGCTGC AGGGCCTGTC CAAGCGCCTG GACGAGCTGC CGCACGTGGA GACGTTCTCG 
ATGCAGATGG ACCTGATCCG GCAGCAGCTT GAGTTCGAGG CCCAGCACAT CCGCTCGCTG 
ATGGAGGAGC GCTTCGGCAC CTCGGACGAG ATGGTGCAGC GGGCACAGAT CCGCGCCTCG 
CGCCTGGAGC AGATTGACAA GGAGCTGCTG GAGGCGCAGG ACCGAGTGCA GCAGACGGAG 
CCCCAGGCCT TGCTGGCGGT GAAGTCGGTG CCGGTGGACG AGGACCCCGA GACAGAGGTC 
CCCACACACC CTGAGGATGG CACCCCTCAG CCGGGCAACA GCAAGGTGGA GGTGGTCTTC 
TGGCTGTTGT CCATGTTGGC GACGCGCGAC CAGGAGGATA CAGCGCGCAC GCTGCTGGCC 
ATGTCCAGCT CGCCCGAGAG CTGCGTGGCC ATGCGCCGCT CGGGCTGTCT GCCTCTGCTG 
CTGCAAATCC TCCACGGCAC CGAGGCCGCG GCCGGGGGTC GCGCCGGGGC CCCAGGGGCA 
CCGGGCGCCA AGGACGCACG CATGCGCGCC AACGCGGCGC TGCACAACAT CGTCTTCTCG 
CAGCCGGACC AGGGCCTGGC GCGCAAGGAG ATGCGCGTCC TGCACGTGCT GGAGCAGATC 
CGGGCCTACT GCGAGACCTG CTGGGACTGG CTGCAGGCCC GAGACGGCGG GCCCGAGGGA 
GGTGGCGCCG GCAGCGCCCC GATCCCCATC GAGCCGCAGA TCTGCCAGGC CACCTGTGCT 
GTTATGAAGC TGTCCTTTGA TGAGGAGTAC CGCCGTGCCA TGAACGAGCT AGGTGGGCTG 
CAGGCCGTGG CAGAGCTGCT GCAGGTTGAC TATGAGATGC ACAAGATGAC CCGGGACCCG 
CTGAACCTGG CGCTGCGCCG CTACGCGGGC ATGACCCTCA CCAACCTCAC CTTTGGGGAC 
GTTGCCAACA AGGCCACCCT GTGTGCGCGC CGCGGCTGCA TGGAGGCCAT CGTGGCCCAG 
CTGGCCTCCG ACAGTGAGGA GCTCCACCAG GTGGTGTCCA GCATCCTTCG GAACTTGTCC 
TGGAGGGCCG ACATCAACAG CAAGAAGGTG CTGAGGGAGG CGGGCAGCGT GACTGCCCTG 
GTGCAGTGTG TCCTGCGGGC CACCAAGGAG TCCACCCTGA AGAGCGTGCT GAGCGCCCTG 
TGGAATCTGT CTGCACACAG CACAGAGAAC AAGGCGGCCA TCTGCCAGGT GGATGGCGCC 
CTGGGCTTCC TGGTGAGCAC CCTGACCTAC AAGTGTCAGA GCAACTCGCT GGCCATCATC 
GAGAGCGGCG GCGGCATCCT CCGCAATGTG TCCAGCCTCG TCGCCACCCG TGAGGACTAC 
AGGCAGGTGC TCCGGGATCA CAACTGTCTG CAGACGCTGC TGCAGCATCT GACTTCGCAC 
AGCCTGACCA TCGTGAGCAA CGCGTGCGGC ACGCTCTGGA ACCTGTCGGC CCGCAGCGCC 
CGTGACCAGG AGCTGCTGTG GGACCTGGGC GCCGTGGGCA TGCTGCGTAA TCTGGTGCAC 
TCCAAGCACA AGATGATCGC CATGGGCAGC GCCGCCGCCC TGCGCAACCT GCTGGCCCAT 
CGGCCCGCCA AGCACCAGGC GGCCGCCACC GCCGTGTCCC CAGGCAGCTG CGTGCCCAGC 
CTGTACGTGC GCAAGCAGCG GGCGCTGGAG GCCGAGCTGG ACGCACGGCA CCTCGCGCAG 
GCGCTGGAGC ACCTGGAGAA GCAGGGCCCG CCGGCAGCCG AGGCCGCCAC TAAGAAGCCG 
CTGCCGCCCC TGCGACACCT GGACGGCCTG GCCCAAGACT ATGCTTCCGA TTCGGGCTGC 
TTTGACGACG ACGATGCACC GTCATCCCTG GCTGCGGCCG CGGCCACCGG GGAGCCAGCC 
AGCCCTGCCG CGCTGTCCCT CTTCCTGGGC AGCCCCTTCC TGCAGGGGCA GGCGCTGGCT 
CGCACCCCGC CCACCCGCCG AGGCGGCAAG GAGGCAGAGA AGGACACCAG TGGGGAGGCA 
GCCGTGGCGG CCAAGGCCAA GGCCAAGCTG GCGCTTGCAG TGGCGCGCAT CGACCAGCTG 
GTGGAGGACA TCTCCGCCCT GCACACCTCG TCCGACGATA GCTTCAGCCT CAGCTCTGGA 
GACCCGGGAC AGGAGGCGCC ACGGGAGGGC CGCGCCCAGT CCTGCTCGCC ATGCCGCGGC 
CCGGAGGGCG GGCGGCGAGA GGCAGGAAGC CGGGCGCACC CGCTGCTGCG GCTCAAGGCG 
GCCCACGCCA GCCTCTCCAA CGACAGCCTC AACAGCGGCA GTGCCAGCGA CGGGTACTGC 
CCACGCGAAC ATATGCTGCC CTGCCCGCTG GCCGCACTGG CTTCGCGCCG CGAGGACCCC 
AGGTGTGGGC AGCCTCGGCC CAGCCGGCTT GACCTTGACC TGCCCGGCTG CCAGGCCGAG 
CCCCCGGCCC GCGAGGCCAC CTCCGCCGAC GCCCGCGTGC GCACCATCAA GCTGTCGCCT 
ACCTATCAGC ACGTGCCACT GCTTGAGGGT GCCTCAAGGG CGGGTGCAGA GCCCCTCGCG 
GGGCCTGGAA TCTCTCCAGG GGCCCGGAAG CAGGCCTGGC TGCCGGCAGA CCACCTGAGC 
AAGGTTCCCG AGAAGCTGGC GGCTGCCCCG CTGTCTGTGG CCAGCAAGGC ACTGCAGAAA 
CTGGCGGCGC AAGAGGGGCC ACTCTCGCTG TCCCGATGCA GCTCCCTTTC CTCGCTGTCC 
TCGGCCGGCC GCCCAGGCCC CAGCGAGGGT GGTGACCTGG ATGACAGTGA CTCCTCCCTG 
GAGGGGCTGG AGGAGGCCGG CCCCAGCGAG GCTGAGCTGG ACAGCACGTG GCGGGCGCCC 
GGGGCCACCT CGCTGCCCGT AGCCATTCCG GCTCCCCGGC GTAACCGAGG CCGGGGCCTG 
GGGGTGGAAG ACGCCACGCC GTCCAGCTCG TCGGAGAACT ACGTGCAGGA GACACCGCTT 
GTGCTGAGCC GCTGCAGCTC TGTGAGCTCG CTGGGCAGCT TCGAGAGCCC GTCCATCGCC 
AGCTCCATCC CCAGTGAACC TTGCAGCGGG CAGGGCAGCG GCACCATCAG CCCTAGCGAG 
CTGCCCGACA GCCCCGGACA GACCATGCCT CCCAGCCGGA GCAAGACGCC ACCGCTGGCG 
CCCGCGCCAC AGGGTCCCCC CGAGGCCACC CAGTTCAGCC TGCAGTGGGA GAGCTACGTG 
AAGCGCTTCC TGGACATCGC CGACTGCCGG GAGCGCTGCC GGCTGCCATC TGAGCTGGAC 
GCAGGCAGCG TGCGCTTTAC CGTGGAGAAG CCAGACGAGA ACTTCTCGTG CGCCTCCAGC 
CTCAGCGCGC TGGCCTTGCA CGAGCACTAC GTGCAGCAGG ACGTGGAGCT GCGGCTGCTG 
CCCTCGGCCT GCCCCGAGCG CGGCGGGGGC GCCGGGGGCG CCGGCCTCCA CTTTGCAGGG 
CACCGGCGGC GGGAGGAGGG GCCGGCGCCC ACGGGTTCTC GCCCTCGCGG CGCCGCGGAC 
CAGGAGCTGG AACTGCTGCG GGAGTGCCTG GGAGCCGCCG TGCCTGCCCG GCTGCGCAAG 
GTGGCCTCCG CGCTGGTGCC AGGTCGCCGC GCACTCCCCG TGCCCGTCTA CATGTTGGTG 
CCCGCCCCGG CCCCGGCCCA GGAGGACGAC TCCTGCACTG ACTCCGCGGA GGGCACGCCG 
GTCAACTTCT CTAGCGCCGC CTCGCTCAGC GACGAGACGC TGCAGGGACC CCCCAGGGAC 
CAGCCCGGGG GACCAGCGGG CAGGCAAAGA CCCACCGGCC GCCCCACCTC TGCCAGACAG 
GCCATGGGGC ACCGGCACAA GGCGGGAGGC GCCGGCCGCA GCGCGGAGCA GTCTCGGGGC 
GCGGGCAAGA ACAGAGCAGG GCTGGAGCTG CCCCTGGGCC GGCCCCCGAG CGCCCCCGCA 
GACAAGGACG GCTCAAAGCC CGGCCGGACC CGCGGGGACG GGGCGCTCCA GTCGCTGTGC 
CTCACGACGC CCACTGAGGA GGCCGTGTAC TGCTTCTACG GCAACGACTC GGACGAGGAG 
CCCCCGGCGG CCGCGCCCAC GCCAACCCAC CGGCGCACAT CGGCCATCCC TCGCGCTTTT 
ACGCGGGAGC GTCCGCAGGG CCGGAAGGAG GCCCCTGCCC CGTCCAAGGC TGCACCAGCT 
GCCCCGCCGC CCGCCCGGAC CCAGCCCAGC CTCATTGCTG ACGAGACCCC GCCCTGCTAC 
TCCCTGAGCT CCTCCGCCAG CTCCCTCAGC GAGCCCGAGC CCTCGGAGCC GCCGGCCGTC 
CATCCACGAG GCCGGGAGCC CGCGGTCACC AAGGACCCGG GCCCAGGAGG CGGACGCGAC 
AGCTCGCCCA GCCCGCGGGC CGCGGAGGAG CTTCTGCAGC GGTGCATCAG CTCGGCCCTG 
CCCAGGCGCC GGCCCCCCGT GTCTGGCCTG CGGCGCCGCA AGCCCCGAGC CACCCGGCTG 
GATGAGCGGC CCGCAGAGGG GTCCCGGGAA CGCGGCGAGG AGGCAGCGGG CTCGGACCGG 
GCCTCCGACC TGGATAGCGT GGAGTGGCGC GCCATCCAGG AGGGCGCCAA TTCAATTGTC 
ACGTGGCTGC ACCAGGCAGC AGCTGCCACG CGGGAGGCCT CGTCCGAGTC CGACTCCATC 
CTGTCCTTCG TATCCGGGCT GTCAGTGGGA TCCACCCTAC AGCCCCCCAA GCACAGGAAG 
GGACGACAGG CGGAGGGAGA AATGGGCAGT GCCCGGCGGC CAGAGAAAAG GGGCGCAGCC 
TCAGTCAAGA CCAGCGGGAG CCCCCGTTCC CCTGCAGGCC CCGAGAAGCC ACGTGGCACA 
CAGAAGACCA CGCCCGGGGT GCCAGCTGTG CTCCGGGGAC GAACAGTGAT CTACGTCCCC 
AGCCCGGCAC CCCGTGCCCA GCCCAAAGGG ACCCCCGGCC CCCGCGCCAC ACCGCGGAAG 
GTGGCGCCCC CTTGCCTGGC ACAGCCCGCG GCTCCAGCCA AAGTCCCGAG CCCCGGGCAG 
CAGCGGTCGC GGAGCCTACA CCGGCCTGCC AAGACCTCGG AGCTGGCGAC GCTGAGCCAG 
CCCCCCAGAA GCGCCACACC GCCCGCCCGC CTCGCCAAGA CCCCCTCCTC CAGCTCCTCC 
CAGACCTCGC CCGCCTCCCA GCCCCTGCCC AGAAAGCGCC CCCCGGTCAC CCAGGCTGCT 
GGGGCCCTGC CCGGCCCCGG AGCCTCCCCG GTGCCCAAAA CGCCGGCGCG CACCCTTCTG 
GCGAAGCAGC ACAAGACGCA GAGATCGCCC GTGCGGATCC CGTTCATGCA GAGGCCGGCC 
CGGCGTGGGC CGCCACCGCT GGCTCGGGCA GTCCCGGAGC CGGGCCCCAG GGGCCGGGCG 
GGGACCGAGG CGGGCCCGGG GGCGCGCGGG GGCCGCCTGG GCCTGGTGCG TGTGGCCTCA 
GCCCTCTCCA GCGGCAGCGA GTCCTCCGAC CGCTCGGGCT TCCGGCGACA GCTAACCTTC 
ATCAAGGAGT CGCCGGGCTT GCGGCGCCGC CGCTCCGAGC TGTCCTCGGC CGAGTCCGCG 
GCCTCTGCCC CCCAGGGCGC CTCGCCCCGC CGCGGCCGGC CCGCGCTGCC CGCCGTCTTC 
CTCTGCTCCT CGCGCTGCGA AGAGCTCCGA GCGGCACCCC GGCAGGGCCC GGCCCCGGCC 
CGGCAGCGGC CCCCCGCGGC CCGACCCAGC CCTGGCGAGC GCCCTGCCCG GCGCACCACC 
TCCGAGAGCC CGTCCCGCCT GCCTGTGCGC GCGCCCGCCG CCCGGCCGGA GACTGTCAAG 
CGCTACGCGT CGCTGCCGCA CATCAGCGTG GCCCGCAGGC CCGACGGCGC CGTCCCCGCG 
GCCCCTGCCT CAGCCGACGC CGCGCGCCGC AGCAGCGACG GGGAGCCCCG GCCGCTCCCC 
AGGGTGGCCG CGCCGGGCAC GACCTGGCGG CGCATCCGAG ATGAGGACGT GCCCCACATC 
CTGCGCAGCA CGCTTCCCGC CACGGCCCTG CCACTGCGGG GCTCCACGCC CGAGGACGCC 
CCGGCCGGGC CCCCGCCGCG CAAGACCAGC GACGCCGTGG TCCAGACCGA GGAGGTCGCC 
GCCCCCAAGA CCAACTCCAG CACGTCCCCG AGCCTGGAGA CCAGGGAGCC CCCCGGGGCC 
CCCGCCGGCG GCCAGCTCTC CCTCCTCGGC AGCGACGTGG ACGGTCCCAG CCTCGCCAAG 
GCTCCCATCT CCGCACCCTT CGTGCACGAG GGCCTGGGGG TCGCCGTGGG GGGCTTCCCC 
GCCAGCCGGC ACGGCTCCCC CAGCCGCTCG GCCCGAGTAC CCCCCTTCAA CTATGTGCCC 
AGCCCCATGG TGGTCGCAGC CACCACCGAC TCGGCCGCGG AGAAAGCCCC GGCCACTGCC 
TCCGCCACCC TCCTGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APC2 ( XM_006722608.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722671.2 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X1, mRNA.

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CloneID	OHu88116
Clone ID Related Accession (Same CDS sequence)	XM_006722608.3
Accession Version	XM_006722608.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722608.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCAG CCCGTCTTCC CGCGCCCTCA TTGGCTGTCT CACCCGCATC CCTCATCCCG 
CATCCTCCCC CGCTCGCGGT GGTCTCGCCC AGCGCTAGGA GCGGCAGCGC CGCCTGCCCA 
GGCCCGGACC GGGCTTTGTC CGCCCCGGAG CCCCTGCCCG CGCCGCGGAG ACCCCGGAGC 
CCGCGCGCTC CGAGGCCACC CCGGGCCGGG ATTTCCGGTG GGGCCCGCGG AGCCGCGCAG 
AGGGAGGAGG CCCCAGACCC AGGCGCCCCG CCAGCCCAGC TGCACACGCT GCAGGAGCTG 
AAGATGGCGA GCTCCGTGGC GCCCTACGAG CAGCTGGTGA GGCAGGTGGA GGCCTTGAAG 
GCTGAGAACA GCCACCTGAG GCAGGAGCTA AGGGACAACT CCAGCCACCT GTCCAAGCTG 
GAGACAGAGA CGTCGGGCAT GAAGGAGGTC CTGAAGCACC TACAGGGAAA ACTGGAGCAG 
GAGGCCCGAG TGCTGGTGTC CTCGGGGCAG ACGGAGGTGC TGGAGCAGCT GAAGGCCCTA 
CAGATGGACA TCACCAGCCT GTACAACCTC AAGTTCCAGC CGCCCACCCT GGGCCCGGAG 
CCTGCCGCCC GGACCCCCGA GGGCAGCCCA GTACACGGCT CCGGGCCCTC CAAGGACAGC 
TTTGGGGAGC TGAGCCGGGC CACCATCCGG CTGCTGGAGG AACTGGACCG GGAACGGTGT 
TTCCTGCTGA ATGAGATTGA GAAGGAGGAG AAGGAGAAGC TCTGGTACTA CTCTCAGCTG 
CAGGGCCTGT CCAAGCGCCT GGACGAGCTG CCGCACGTGG AGACGCAGTT CTCGATGCAG 
ATGGACCTGA TCCGGCAGCA GCTTGAGTTC GAGGCCCAGC ACATCCGCTC GCTGATGGAG 
GAGCGCTTCG GCACCTCGGA CGAGATGGTG CAGCGGGCAC AGATCCGCGC CTCGCGCCTG 
GAGCAGATTG ACAAGGAGCT GCTGGAGGCG CAGGACCGAG TGCAGCAGAC GGAGCCCCAG 
GCCTTGCTGG CGGTGAAGTC GGTGCCGGTG GACGAGGACC CCGAGACAGA GGTCCCCACA 
CACCCTGAGG ATGGCACCCC TCAGCCGGGC AACAGCAAGG TGGAGGTGGT CTTCTGGCTG 
TTGTCCATGT TGGCGACGCG CGACCAGGAG GATACAGCGC GCACGCTGCT GGCCATGTCC 
AGCTCGCCCG AGAGCTGCGT GGCCATGCGC CGCTCGGGCT GTCTGCCTCT GCTGCTGCAA 
ATCCTCCACG GCACCGAGGC CGCGGCCGGG GGTCGCGCCG GGGCCCCAGG GGCACCGGGC 
GCCAAGGACG CACGCATGCG CGCCAACGCG GCGCTGCACA ACATCGTCTT CTCGCAGCCG 
GACCAGGGCC TGGCGCGCAA GGAGATGCGC GTCCTGCACG TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA CTGGCTGCAG GCCCGAGACG GCGGGCCCGA GGGAGGTGGC 
GCCGGCAGCG CCCCGATCCC CATCGAGCCG CAGATCTGCC AGGCCACCTG TGCTGTTATG 
AAGCTGTCCT TTGATGAGGA GTACCGCCGT GCCATGAACG AGCTAGGTGG GCTGCAGGCC 
GTGGCAGAGC TGCTGCAGGT TGACTATGAG ATGCACAAGA TGACCCGGGA CCCGCTGAAC 
CTGGCGCTGC GCCGCTACGC GGGCATGACC CTCACCAACC TCACCTTTGG GGACGTTGCC 
AACAAGGCCA CCCTGTGTGC GCGCCGCGGC TGCATGGAGG CCATCGTGGC CCAGCTGGCC 
TCCGACAGTG AGGAGCTCCA CCAGGTGGTG TCCAGCATCC TTCGGAACTT GTCCTGGAGG 
GCCGACATCA ACAGCAAGAA GGTGCTGAGG GAGGCGGGCA GCGTGACTGC CCTGGTGCAG 
TGTGTCCTGC GGGCCACCAA GGAGTCCACC CTGAAGAGCG TGCTGAGCGC CCTGTGGAAT 
CTGTCTGCAC ACAGCACAGA GAACAAGGCG GCCATCTGCC AGGTGGATGG CGCCCTGGGC 
TTCCTGGTGA GCACCCTGAC CTACAAGTGT CAGAGCAACT CGCTGGCCAT CATCGAGAGC 
GGCGGCGGCA TCCTCCGCAA TGTGTCCAGC CTCGTCGCCA CCCGTGAGGA CTACAGGCAG 
GTGCTCCGGG ATCACAACTG TCTGCAGACG CTGCTGCAGC ATCTGACTTC GCACAGCCTG 
ACCATCGTGA GCAACGCGTG CGGCACGCTC TGGAACCTGT CGGCCCGCAG CGCCCGTGAC 
CAGGAGCTGC TGTGGGACCT GGGCGCCGTG GGCATGCTGC GTAATCTGGT GCACTCCAAG 
CACAAGATGA TCGCCATGGG CAGCGCCGCC GCCCTGCGCA ACCTGCTGGC CCATCGGCCC 
GCCAAGCACC AGGCGGCCGC CACCGCCGTG TCCCCAGGCA GCTGCGTGCC CAGCCTGTAC 
GTGCGCAAGC AGCGGGCGCT GGAGGCCGAG CTGGACGCAC GGCACCTCGC GCAGGCGCTG 
GAGCACCTGG AGAAGCAGGG CCCGCCGGCA GCCGAGGCCG CCACTAAGAA GCCGCTGCCG 
CCCCTGCGAC ACCTGGACGG CCTGGCCCAA GACTATGCTT CCGATTCGGG CTGCTTTGAC 
GACGACGATG CACCGTCATC CCTGGCTGCG GCCGCGGCCA CCGGGGAGCC AGCCAGCCCT 
GCCGCGCTGT CCCTCTTCCT GGGCAGCCCC TTCCTGCAGG GGCAGGCGCT GGCTCGCACC 
CCGCCCACCC GCCGAGGCGG CAAGGAGGCA GAGAAGGACA CCAGTGGGGA GGCAGCCGTG 
GCGGCCAAGG CCAAGGCCAA GCTGGCGCTT GCAGTGGCGC GCATCGACCA GCTGGTGGAG 
GACATCTCCG CCCTGCACAC CTCGTCCGAC GATAGCTTCA GCCTCAGCTC TGGAGACCCG 
GGACAGGAGG CGCCACGGGA GGGCCGCGCC CAGTCCTGCT CGCCATGCCG CGGCCCGGAG 
GGCGGGCGGC GAGAGGCAGG AAGCCGGGCG CACCCGCTGC TGCGGCTCAA GGCGGCCCAC 
GCCAGCCTCT CCAACGACAG CCTCAACAGC GGCAGTGCCA GCGACGGGTA CTGCCCACGC 
GAACATATGC TGCCCTGCCC GCTGGCCGCA CTGGCTTCGC GCCGCGAGGA CCCCAGGTGT 
GGGCAGCCTC GGCCCAGCCG GCTTGACCTT GACCTGCCCG GCTGCCAGGC CGAGCCCCCG 
GCCCGCGAGG CCACCTCCGC CGACGCCCGC GTGCGCACCA TCAAGCTGTC GCCTACCTAT 
CAGCACGTGC CACTGCTTGA GGGTGCCTCA AGGGCGGGTG CAGAGCCCCT CGCGGGGCCT 
GGAATCTCTC CAGGGGCCCG GAAGCAGGCC TGGCTGCCGG CAGACCACCT GAGCAAGGTT 
CCCGAGAAGC TGGCGGCTGC CCCGCTGTCT GTGGCCAGCA AGGCACTGCA GAAACTGGCG 
GCGCAAGAGG GGCCACTCTC GCTGTCCCGA TGCAGCTCCC TTTCCTCGCT GTCCTCGGCC 
GGCCGCCCAG GCCCCAGCGA GGGTGGTGAC CTGGATGACA GTGACTCCTC CCTGGAGGGG 
CTGGAGGAGG CCGGCCCCAG CGAGGCTGAG CTGGACAGCA CGTGGCGGGC GCCCGGGGCC 
ACCTCGCTGC CCGTAGCCAT TCCGGCTCCC CGGCGTAACC GAGGCCGGGG CCTGGGGGTG 
GAAGACGCCA CGCCGTCCAG CTCGTCGGAG AACTACGTGC AGGAGACACC GCTTGTGCTG 
AGCCGCTGCA GCTCTGTGAG CTCGCTGGGC AGCTTCGAGA GCCCGTCCAT CGCCAGCTCC 
ATCCCCAGTG AACCTTGCAG CGGGCAGGGC AGCGGCACCA TCAGCCCTAG CGAGCTGCCC 
GACAGCCCCG GACAGACCAT GCCTCCCAGC CGGAGCAAGA CGCCACCGCT GGCGCCCGCG 
CCACAGGGTC CCCCCGAGGC CACCCAGTTC AGCCTGCAGT GGGAGAGCTA CGTGAAGCGC 
TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CATCTGAGCT GGACGCAGGC 
AGCGTGCGCT TTACCGTGGA GAAGCCAGAC GAGAACTTCT CGTGCGCCTC CAGCCTCAGC 
GCGCTGGCCT TGCACGAGCA CTACGTGCAG CAGGACGTGG AGCTGCGGCT GCTGCCCTCG 
GCCTGCCCCG AGCGCGGCGG GGGCGCCGGG GGCGCCGGCC TCCACTTTGC AGGGCACCGG 
CGGCGGGAGG AGGGGCCGGC GCCCACGGGT TCTCGCCCTC GCGGCGCCGC GGACCAGGAG 
CTGGAACTGC TGCGGGAGTG CCTGGGAGCC GCCGTGCCTG CCCGGCTGCG CAAGGTGGCC 
TCCGCGCTGG TGCCAGGTCG CCGCGCACTC CCCGTGCCCG TCTACATGTT GGTGCCCGCC 
CCGGCCCCGG CCCAGGAGGA CGACTCCTGC ACTGACTCCG CGGAGGGCAC GCCGGTCAAC 
TTCTCTAGCG CCGCCTCGCT CAGCGACGAG ACGCTGCAGG GACCCCCCAG GGACCAGCCC 
GGGGGACCAG CGGGCAGGCA AAGACCCACC GGCCGCCCCA CCTCTGCCAG ACAGGCCATG 
GGGCACCGGC ACAAGGCGGG AGGCGCCGGC CGCAGCGCGG AGCAGTCTCG GGGCGCGGGC 
AAGAACAGAG CAGGGCTGGA GCTGCCCCTG GGCCGGCCCC CGAGCGCCCC CGCAGACAAG 
GACGGCTCAA AGCCCGGCCG GACCCGCGGG GACGGGGCGC TCCAGTCGCT GTGCCTCACG 
ACGCCCACTG AGGAGGCCGT GTACTGCTTC TACGGCAACG ACTCGGACGA GGAGCCCCCG 
GCGGCCGCGC CCACGCCAAC CCACCGGCGC ACATCGGCCA TCCCTCGCGC TTTTACGCGG 
GAGCGTCCGC AGGGCCGGAA GGAGGCCCCT GCCCCGTCCA AGGCTGCACC AGCTGCCCCG 
CCGCCCGCCC GGACCCAGCC CAGCCTCATT GCTGACGAGA CCCCGCCCTG CTACTCCCTG 
AGCTCCTCCG CCAGCTCCCT CAGCGAGCCC GAGCCCTCGG AGCCGCCGGC CGTCCATCCA 
CGAGGCCGGG AGCCCGCGGT CACCAAGGAC CCGGGCCCAG GAGGCGGACG CGACAGCTCG 
CCCAGCCCGC GGGCCGCGGA GGAGCTTCTG CAGCGGTGCA TCAGCTCGGC CCTGCCCAGG 
CGCCGGCCCC CCGTGTCTGG CCTGCGGCGC CGCAAGCCCC GAGCCACCCG GCTGGATGAG 
CGGCCCGCAG AGGGGTCCCG GGAACGCGGC GAGGAGGCAG CGGGCTCGGA CCGGGCCTCC 
GACCTGGATA GCGTGGAGTG GCGCGCCATC CAGGAGGGCG CCAATTCAAT TGTCACGTGG 
CTGCACCAGG CAGCAGCTGC CACGCGGGAG GCCTCGTCCG AGTCCGACTC CATCCTGTCC 
TTCGTATCCG GGCTGTCAGT GGGATCCACC CTACAGCCCC CCAAGCACAG GAAGGGACGA 
CAGGCGGAGG GAGAAATGGG CAGTGCCCGG CGGCCAGAGA AAAGGGGCGC AGCCTCAGTC 
AAGACCAGCG GGAGCCCCCG TTCCCCTGCA GGCCCCGAGA AGCCACGTGG CACACAGAAG 
ACCACGCCCG GGGTGCCAGC TGTGCTCCGG GGACGAACAG TGATCTACGT CCCCAGCCCG 
GCACCCCGTG CCCAGCCCAA AGGGACCCCC GGCCCCCGCG CCACACCGCG GAAGGTGGCG 
CCCCCTTGCC TGGCACAGCC CGCGGCTCCA GCCAAAGTCC CGAGCCCCGG GCAGCAGCGG 
TCGCGGAGCC TACACCGGCC TGCCAAGACC TCGGAGCTGG CGACGCTGAG CCAGCCCCCC 
AGAAGCGCCA CACCGCCCGC CCGCCTCGCC AAGACCCCCT CCTCCAGCTC CTCCCAGACC 
TCGCCCGCCT CCCAGCCCCT GCCCAGAAAG CGCCCCCCGG TCACCCAGGC TGCTGGGGCC 
CTGCCCGGCC CCGGAGCCTC CCCGGTGCCC AAAACGCCGG CGCGCACCCT TCTGGCGAAG 
CAGCACAAGA CGCAGAGATC GCCCGTGCGG ATCCCGTTCA TGCAGAGGCC GGCCCGGCGT 
GGGCCGCCAC CGCTGGCTCG GGCAGTCCCG GAGCCGGGCC CCAGGGGCCG GGCGGGGACC 
GAGGCGGGCC CGGGGGCGCG CGGGGGCCGC CTGGGCCTGG TGCGTGTGGC CTCAGCCCTC 
TCCAGCGGCA GCGAGTCCTC CGACCGCTCG GGCTTCCGGC GACAGCTAAC CTTCATCAAG 
GAGTCGCCGG GCTTGCGGCG CCGCCGCTCC GAGCTGTCCT CGGCCGAGTC CGCGGCCTCT 
GCCCCCCAGG GCGCCTCGCC CCGCCGCGGC CGGCCCGCGC TGCCCGCCGT CTTCCTCTGC 
TCCTCGCGCT GCGAAGAGCT CCGAGCGGCA CCCCGGCAGG GCCCGGCCCC GGCCCGGCAG 
CGGCCCCCCG CGGCCCGACC CAGCCCTGGC GAGCGCCCTG CCCGGCGCAC CACCTCCGAG 
AGCCCGTCCC GCCTGCCTGT GCGCGCGCCC GCCGCCCGGC CGGAGACTGT CAAGCGCTAC 
GCGTCGCTGC CGCACATCAG CGTGGCCCGC AGGCCCGACG GCGCCGTCCC CGCGGCCCCT 
GCCTCAGCCG ACGCCGCGCG CCGCAGCAGC GACGGGGAGC CCCGGCCGCT CCCCAGGGTG 
GCCGCGCCGG GCACGACCTG GCGGCGCATC CGAGATGAGG ACGTGCCCCA CATCCTGCGC 
AGCACGCTTC CCGCCACGGC CCTGCCACTG CGGGGCTCCA CGCCCGAGGA CGCCCCGGCC 
GGGCCCCCGC CGCGCAAGAC CAGCGACGCC GTGGTCCAGA CCGAGGAGGT CGCCGCCCCC 
AAGACCAACT CCAGCACGTC CCCGAGCCTG GAGACCAGGG AGCCCCCCGG GGCCCCCGCC 
GGCGGCCAGC TCTCCCTCCT CGGCAGCGAC GTGGACGGTC CCAGCCTCGC CAAGGCTCCC 
ATCTCCGCAC CCTTCGTGCA CGAGGGCCTG GGGGTCGCCG TGGGGGGCTT CCCCGCCAGC 
CGGCACGGCT CCCCCAGCCG CTCGGCCCGA GTACCCCCCT TCAACTATGT GCCCAGCCCC 
ATGGTGGTCG CAGCCACCAC CGACTCGGCC GCGGAGAAAG CCCCGGCCAC TGCCTCCGCC 
ACCCTCCTGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722671.2
CDS	51..7265
Translation	MAPARLPAPSLAVSPASLIPHPPPLAVVSPSARSGSAACPGPDRALSAPEPLPAPRRPRSPRAPRPPRAGISGGARGAAQREEAPDPGAPPAQLHTLQELKMASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETQFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	XM_006722608.3
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722608.3

ORF Insert Sequence:

ATGGCACCAG CCCGTCTTCC CGCGCCCTCA TTGGCTGTCT CACCCGCATC CCTCATCCCG 
CATCCTCCCC CGCTCGCGGT GGTCTCGCCC AGCGCTAGGA GCGGCAGCGC CGCCTGCCCA 
GGCCCGGACC GGGCTTTGTC CGCCCCGGAG CCCCTGCCCG CGCCGCGGAG ACCCCGGAGC 
CCGCGCGCTC CGAGGCCACC CCGGGCCGGG ATTTCCGGTG GGGCCCGCGG AGCCGCGCAG 
AGGGAGGAGG CCCCAGACCC AGGCGCCCCG CCAGCCCAGC TGCACACGCT GCAGGAGCTG 
AAGATGGCGA GCTCCGTGGC GCCCTACGAG CAGCTGGTGA GGCAGGTGGA GGCCTTGAAG 
GCTGAGAACA GCCACCTGAG GCAGGAGCTA AGGGACAACT CCAGCCACCT GTCCAAGCTG 
GAGACAGAGA CGTCGGGCAT GAAGGAGGTC CTGAAGCACC TACAGGGAAA ACTGGAGCAG 
GAGGCCCGAG TGCTGGTGTC CTCGGGGCAG ACGGAGGTGC TGGAGCAGCT GAAGGCCCTA 
CAGATGGACA TCACCAGCCT GTACAACCTC AAGTTCCAGC CGCCCACCCT GGGCCCGGAG 
CCTGCCGCCC GGACCCCCGA GGGCAGCCCA GTACACGGCT CCGGGCCCTC CAAGGACAGC 
TTTGGGGAGC TGAGCCGGGC CACCATCCGG CTGCTGGAGG AACTGGACCG GGAACGGTGT 
TTCCTGCTGA ATGAGATTGA GAAGGAGGAG AAGGAGAAGC TCTGGTACTA CTCTCAGCTG 
CAGGGCCTGT CCAAGCGCCT GGACGAGCTG CCGCACGTGG AGACGCAGTT CTCGATGCAG 
ATGGACCTGA TCCGGCAGCA GCTTGAGTTC GAGGCCCAGC ACATCCGCTC GCTGATGGAG 
GAGCGCTTCG GCACCTCGGA CGAGATGGTG CAGCGGGCAC AGATCCGCGC CTCGCGCCTG 
GAGCAGATTG ACAAGGAGCT GCTGGAGGCG CAGGACCGAG TGCAGCAGAC GGAGCCCCAG 
GCCTTGCTGG CGGTGAAGTC GGTGCCGGTG GACGAGGACC CCGAGACAGA GGTCCCCACA 
CACCCTGAGG ATGGCACCCC TCAGCCGGGC AACAGCAAGG TGGAGGTGGT CTTCTGGCTG 
TTGTCCATGT TGGCGACGCG CGACCAGGAG GATACAGCGC GCACGCTGCT GGCCATGTCC 
AGCTCGCCCG AGAGCTGCGT GGCCATGCGC CGCTCGGGCT GTCTGCCTCT GCTGCTGCAA 
ATCCTCCACG GCACCGAGGC CGCGGCCGGG GGTCGCGCCG GGGCCCCAGG GGCACCGGGC 
GCCAAGGACG CACGCATGCG CGCCAACGCG GCGCTGCACA ACATCGTCTT CTCGCAGCCG 
GACCAGGGCC TGGCGCGCAA GGAGATGCGC GTCCTGCACG TGCTGGAGCA GATCCGGGCC 
TACTGCGAGA CCTGCTGGGA CTGGCTGCAG GCCCGAGACG GCGGGCCCGA GGGAGGTGGC 
GCCGGCAGCG CCCCGATCCC CATCGAGCCG CAGATCTGCC AGGCCACCTG TGCTGTTATG 
AAGCTGTCCT TTGATGAGGA GTACCGCCGT GCCATGAACG AGCTAGGTGG GCTGCAGGCC 
GTGGCAGAGC TGCTGCAGGT TGACTATGAG ATGCACAAGA TGACCCGGGA CCCGCTGAAC 
CTGGCGCTGC GCCGCTACGC GGGCATGACC CTCACCAACC TCACCTTTGG GGACGTTGCC 
AACAAGGCCA CCCTGTGTGC GCGCCGCGGC TGCATGGAGG CCATCGTGGC CCAGCTGGCC 
TCCGACAGTG AGGAGCTCCA CCAGGTGGTG TCCAGCATCC TTCGGAACTT GTCCTGGAGG 
GCCGACATCA ACAGCAAGAA GGTGCTGAGG GAGGCGGGCA GCGTGACTGC CCTGGTGCAG 
TGTGTCCTGC GGGCCACCAA GGAGTCCACC CTGAAGAGCG TGCTGAGCGC CCTGTGGAAT 
CTGTCTGCAC ACAGCACAGA GAACAAGGCG GCCATCTGCC AGGTGGATGG CGCCCTGGGC 
TTCCTGGTGA GCACCCTGAC CTACAAGTGT CAGAGCAACT CGCTGGCCAT CATCGAGAGC 
GGCGGCGGCA TCCTCCGCAA TGTGTCCAGC CTCGTCGCCA CCCGTGAGGA CTACAGGCAG 
GTGCTCCGGG ATCACAACTG TCTGCAGACG CTGCTGCAGC ATCTGACTTC GCACAGCCTG 
ACCATCGTGA GCAACGCGTG CGGCACGCTC TGGAACCTGT CGGCCCGCAG CGCCCGTGAC 
CAGGAGCTGC TGTGGGACCT GGGCGCCGTG GGCATGCTGC GTAATCTGGT GCACTCCAAG 
CACAAGATGA TCGCCATGGG CAGCGCCGCC GCCCTGCGCA ACCTGCTGGC CCATCGGCCC 
GCCAAGCACC AGGCGGCCGC CACCGCCGTG TCCCCAGGCA GCTGCGTGCC CAGCCTGTAC 
GTGCGCAAGC AGCGGGCGCT GGAGGCCGAG CTGGACGCAC GGCACCTCGC GCAGGCGCTG 
GAGCACCTGG AGAAGCAGGG CCCGCCGGCA GCCGAGGCCG CCACTAAGAA GCCGCTGCCG 
CCCCTGCGAC ACCTGGACGG CCTGGCCCAA GACTATGCTT CCGATTCGGG CTGCTTTGAC 
GACGACGATG CACCGTCATC CCTGGCTGCG GCCGCGGCCA CCGGGGAGCC AGCCAGCCCT 
GCCGCGCTGT CCCTCTTCCT GGGCAGCCCC TTCCTGCAGG GGCAGGCGCT GGCTCGCACC 
CCGCCCACCC GCCGAGGCGG CAAGGAGGCA GAGAAGGACA CCAGTGGGGA GGCAGCCGTG 
GCGGCCAAGG CCAAGGCCAA GCTGGCGCTT GCAGTGGCGC GCATCGACCA GCTGGTGGAG 
GACATCTCCG CCCTGCACAC CTCGTCCGAC GATAGCTTCA GCCTCAGCTC TGGAGACCCG 
GGACAGGAGG CGCCACGGGA GGGCCGCGCC CAGTCCTGCT CGCCATGCCG CGGCCCGGAG 
GGCGGGCGGC GAGAGGCAGG AAGCCGGGCG CACCCGCTGC TGCGGCTCAA GGCGGCCCAC 
GCCAGCCTCT CCAACGACAG CCTCAACAGC GGCAGTGCCA GCGACGGGTA CTGCCCACGC 
GAACATATGC TGCCCTGCCC GCTGGCCGCA CTGGCTTCGC GCCGCGAGGA CCCCAGGTGT 
GGGCAGCCTC GGCCCAGCCG GCTTGACCTT GACCTGCCCG GCTGCCAGGC CGAGCCCCCG 
GCCCGCGAGG CCACCTCCGC CGACGCCCGC GTGCGCACCA TCAAGCTGTC GCCTACCTAT 
CAGCACGTGC CACTGCTTGA GGGTGCCTCA AGGGCGGGTG CAGAGCCCCT CGCGGGGCCT 
GGAATCTCTC CAGGGGCCCG GAAGCAGGCC TGGCTGCCGG CAGACCACCT GAGCAAGGTT 
CCCGAGAAGC TGGCGGCTGC CCCGCTGTCT GTGGCCAGCA AGGCACTGCA GAAACTGGCG 
GCGCAAGAGG GGCCACTCTC GCTGTCCCGA TGCAGCTCCC TTTCCTCGCT GTCCTCGGCC 
GGCCGCCCAG GCCCCAGCGA GGGTGGTGAC CTGGATGACA GTGACTCCTC CCTGGAGGGG 
CTGGAGGAGG CCGGCCCCAG CGAGGCTGAG CTGGACAGCA CGTGGCGGGC GCCCGGGGCC 
ACCTCGCTGC CCGTAGCCAT TCCGGCTCCC CGGCGTAACC GAGGCCGGGG CCTGGGGGTG 
GAAGACGCCA CGCCGTCCAG CTCGTCGGAG AACTACGTGC AGGAGACACC GCTTGTGCTG 
AGCCGCTGCA GCTCTGTGAG CTCGCTGGGC AGCTTCGAGA GCCCGTCCAT CGCCAGCTCC 
ATCCCCAGTG AACCTTGCAG CGGGCAGGGC AGCGGCACCA TCAGCCCTAG CGAGCTGCCC 
GACAGCCCCG GACAGACCAT GCCTCCCAGC CGGAGCAAGA CGCCACCGCT GGCGCCCGCG 
CCACAGGGTC CCCCCGAGGC CACCCAGTTC AGCCTGCAGT GGGAGAGCTA CGTGAAGCGC 
TTCCTGGACA TCGCCGACTG CCGGGAGCGC TGCCGGCTGC CATCTGAGCT GGACGCAGGC 
AGCGTGCGCT TTACCGTGGA GAAGCCAGAC GAGAACTTCT CGTGCGCCTC CAGCCTCAGC 
GCGCTGGCCT TGCACGAGCA CTACGTGCAG CAGGACGTGG AGCTGCGGCT GCTGCCCTCG 
GCCTGCCCCG AGCGCGGCGG GGGCGCCGGG GGCGCCGGCC TCCACTTTGC AGGGCACCGG 
CGGCGGGAGG AGGGGCCGGC GCCCACGGGT TCTCGCCCTC GCGGCGCCGC GGACCAGGAG 
CTGGAACTGC TGCGGGAGTG CCTGGGAGCC GCCGTGCCTG CCCGGCTGCG CAAGGTGGCC 
TCCGCGCTGG TGCCAGGTCG CCGCGCACTC CCCGTGCCCG TCTACATGTT GGTGCCCGCC 
CCGGCCCCGG CCCAGGAGGA CGACTCCTGC ACTGACTCCG CGGAGGGCAC GCCGGTCAAC 
TTCTCTAGCG CCGCCTCGCT CAGCGACGAG ACGCTGCAGG GACCCCCCAG GGACCAGCCC 
GGGGGACCAG CGGGCAGGCA AAGACCCACC GGCCGCCCCA CCTCTGCCAG ACAGGCCATG 
GGGCACCGGC ACAAGGCGGG AGGCGCCGGC CGCAGCGCGG AGCAGTCTCG GGGCGCGGGC 
AAGAACAGAG CAGGGCTGGA GCTGCCCCTG GGCCGGCCCC CGAGCGCCCC CGCAGACAAG 
GACGGCTCAA AGCCCGGCCG GACCCGCGGG GACGGGGCGC TCCAGTCGCT GTGCCTCACG 
ACGCCCACTG AGGAGGCCGT GTACTGCTTC TACGGCAACG ACTCGGACGA GGAGCCCCCG 
GCGGCCGCGC CCACGCCAAC CCACCGGCGC ACATCGGCCA TCCCTCGCGC TTTTACGCGG 
GAGCGTCCGC AGGGCCGGAA GGAGGCCCCT GCCCCGTCCA AGGCTGCACC AGCTGCCCCG 
CCGCCCGCCC GGACCCAGCC CAGCCTCATT GCTGACGAGA CCCCGCCCTG CTACTCCCTG 
AGCTCCTCCG CCAGCTCCCT CAGCGAGCCC GAGCCCTCGG AGCCGCCGGC CGTCCATCCA 
CGAGGCCGGG AGCCCGCGGT CACCAAGGAC CCGGGCCCAG GAGGCGGACG CGACAGCTCG 
CCCAGCCCGC GGGCCGCGGA GGAGCTTCTG CAGCGGTGCA TCAGCTCGGC CCTGCCCAGG 
CGCCGGCCCC CCGTGTCTGG CCTGCGGCGC CGCAAGCCCC GAGCCACCCG GCTGGATGAG 
CGGCCCGCAG AGGGGTCCCG GGAACGCGGC GAGGAGGCAG CGGGCTCGGA CCGGGCCTCC 
GACCTGGATA GCGTGGAGTG GCGCGCCATC CAGGAGGGCG CCAATTCAAT TGTCACGTGG 
CTGCACCAGG CAGCAGCTGC CACGCGGGAG GCCTCGTCCG AGTCCGACTC CATCCTGTCC 
TTCGTATCCG GGCTGTCAGT GGGATCCACC CTACAGCCCC CCAAGCACAG GAAGGGACGA 
CAGGCGGAGG GAGAAATGGG CAGTGCCCGG CGGCCAGAGA AAAGGGGCGC AGCCTCAGTC 
AAGACCAGCG GGAGCCCCCG TTCCCCTGCA GGCCCCGAGA AGCCACGTGG CACACAGAAG 
ACCACGCCCG GGGTGCCAGC TGTGCTCCGG GGACGAACAG TGATCTACGT CCCCAGCCCG 
GCACCCCGTG CCCAGCCCAA AGGGACCCCC GGCCCCCGCG CCACACCGCG GAAGGTGGCG 
CCCCCTTGCC TGGCACAGCC CGCGGCTCCA GCCAAAGTCC CGAGCCCCGG GCAGCAGCGG 
TCGCGGAGCC TACACCGGCC TGCCAAGACC TCGGAGCTGG CGACGCTGAG CCAGCCCCCC 
AGAAGCGCCA CACCGCCCGC CCGCCTCGCC AAGACCCCCT CCTCCAGCTC CTCCCAGACC 
TCGCCCGCCT CCCAGCCCCT GCCCAGAAAG CGCCCCCCGG TCACCCAGGC TGCTGGGGCC 
CTGCCCGGCC CCGGAGCCTC CCCGGTGCCC AAAACGCCGG CGCGCACCCT TCTGGCGAAG 
CAGCACAAGA CGCAGAGATC GCCCGTGCGG ATCCCGTTCA TGCAGAGGCC GGCCCGGCGT 
GGGCCGCCAC CGCTGGCTCG GGCAGTCCCG GAGCCGGGCC CCAGGGGCCG GGCGGGGACC 
GAGGCGGGCC CGGGGGCGCG CGGGGGCCGC CTGGGCCTGG TGCGTGTGGC CTCAGCCCTC 
TCCAGCGGCA GCGAGTCCTC CGACCGCTCG GGCTTCCGGC GACAGCTAAC CTTCATCAAG 
GAGTCGCCGG GCTTGCGGCG CCGCCGCTCC GAGCTGTCCT CGGCCGAGTC CGCGGCCTCT 
GCCCCCCAGG GCGCCTCGCC CCGCCGCGGC CGGCCCGCGC TGCCCGCCGT CTTCCTCTGC 
TCCTCGCGCT GCGAAGAGCT CCGAGCGGCA CCCCGGCAGG GCCCGGCCCC GGCCCGGCAG 
CGGCCCCCCG CGGCCCGACC CAGCCCTGGC GAGCGCCCTG CCCGGCGCAC CACCTCCGAG 
AGCCCGTCCC GCCTGCCTGT GCGCGCGCCC GCCGCCCGGC CGGAGACTGT CAAGCGCTAC 
GCGTCGCTGC CGCACATCAG CGTGGCCCGC AGGCCCGACG GCGCCGTCCC CGCGGCCCCT 
GCCTCAGCCG ACGCCGCGCG CCGCAGCAGC GACGGGGAGC CCCGGCCGCT CCCCAGGGTG 
GCCGCGCCGG GCACGACCTG GCGGCGCATC CGAGATGAGG ACGTGCCCCA CATCCTGCGC 
AGCACGCTTC CCGCCACGGC CCTGCCACTG CGGGGCTCCA CGCCCGAGGA CGCCCCGGCC 
GGGCCCCCGC CGCGCAAGAC CAGCGACGCC GTGGTCCAGA CCGAGGAGGT CGCCGCCCCC 
AAGACCAACT CCAGCACGTC CCCGAGCCTG GAGACCAGGG AGCCCCCCGG GGCCCCCGCC 
GGCGGCCAGC TCTCCCTCCT CGGCAGCGAC GTGGACGGTC CCAGCCTCGC CAAGGCTCCC 
ATCTCCGCAC CCTTCGTGCA CGAGGGCCTG GGGGTCGCCG TGGGGGGCTT CCCCGCCAGC 
CGGCACGGCT CCCCCAGCCG CTCGGCCCGA GTACCCCCCT TCAACTATGT GCCCAGCCCC 
ATGGTGGTCG CAGCCACCAC CGACTCGGCC GCGGAGAAAG CCCCGGCCAC TGCCTCCGCC 
ACCCTCCTGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APC2 ( XM_006722610.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722673.2 Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X2, mRNA.

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CloneID	OHu88117
Clone ID Related Accession (Same CDS sequence)	XM_006722610.3
Accession Version	XM_006722610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adenomatous polyposis coli protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722610.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCAG CCCGTCTTCC CGCGCCCTCA TTGGCTGTCT CACCCGCATC CCTCATCCCG 
CATCCTCCCC CGCTCGCGGT GGTCTCGCCC AGCGCTAGGA GCGGCAGCGC CGCCTGCCCA 
GGCCCGGACC GGGCTTTGTC CGCCCCGGAG CCCCTGCCCG CGCCGCGGAG ACCCCGGAGC 
CCGCGCGCTC CGAGGCCACC CCGGGCCGGG ATTTCCGGTG GGGCCCGCGG AGCCGCGCAG 
AGGGAGGAGG CCCCAGACCC AGGCGCCCCG CCAGCCCAGC TGCACACGCT GCAGGAGCTG 
AAGATGGCGA GCTCCGTGGC GCCCTACGAG CAGCTGGTGA GGCAGGTGGA GGCCTTGAAG 
GCTGAGAACA GCCACCTGAG GCAGGAGCTA AGGGACAACT CCAGCCACCT GTCCAAGCTG 
GAGACAGAGA CGTCGGGCAT GAAGGAGGTC CTGAAGCACC TACAGGGAAA ACTGGAGCAG 
GAGGCCCGAG TGCTGGTGTC CTCGGGGCAG ACGGAGGTGC TGGAGCAGCT GAAGGCCCTA 
CAGATGGACA TCACCAGCCT GTACAACCTC AAGTTCCAGC CGCCCACCCT GGGCCCGGAG 
CCTGCCGCCC GGACCCCCGA GGGCAGCCCA GTACACGGCT CCGGGCCCTC CAAGGACAGC 
TTTGGGGAGC TGAGCCGGGC CACCATCCGG CTGCTGGAGG AACTGGACCG GGAACGGTGT 
TTCCTGCTGA ATGAGATTGA GAAGGAGGAG AAGGAGAAGC TCTGGTACTA CTCTCAGCTG 
CAGGGCCTGT CCAAGCGCCT GGACGAGCTG CCGCACGTGG AGACGTTCTC GATGCAGATG 
GACCTGATCC GGCAGCAGCT TGAGTTCGAG GCCCAGCACA TCCGCTCGCT GATGGAGGAG 
CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCACAGA TCCGCGCCTC GCGCCTGGAG 
CAGATTGACA AGGAGCTGCT GGAGGCGCAG GACCGAGTGC AGCAGACGGA GCCCCAGGCC 
TTGCTGGCGG TGAAGTCGGT GCCGGTGGAC GAGGACCCCG AGACAGAGGT CCCCACACAC 
CCTGAGGATG GCACCCCTCA GCCGGGCAAC AGCAAGGTGG AGGTGGTCTT CTGGCTGTTG 
TCCATGTTGG CGACGCGCGA CCAGGAGGAT ACAGCGCGCA CGCTGCTGGC CATGTCCAGC 
TCGCCCGAGA GCTGCGTGGC CATGCGCCGC TCGGGCTGTC TGCCTCTGCT GCTGCAAATC 
CTCCACGGCA CCGAGGCCGC GGCCGGGGGT CGCGCCGGGG CCCCAGGGGC ACCGGGCGCC 
AAGGACGCAC GCATGCGCGC CAACGCGGCG CTGCACAACA TCGTCTTCTC GCAGCCGGAC 
CAGGGCCTGG CGCGCAAGGA GATGCGCGTC CTGCACGTGC TGGAGCAGAT CCGGGCCTAC 
TGCGAGACCT GCTGGGACTG GCTGCAGGCC CGAGACGGCG GGCCCGAGGG AGGTGGCGCC 
GGCAGCGCCC CGATCCCCAT CGAGCCGCAG ATCTGCCAGG CCACCTGTGC TGTTATGAAG 
CTGTCCTTTG ATGAGGAGTA CCGCCGTGCC ATGAACGAGC TAGGTGGGCT GCAGGCCGTG 
GCAGAGCTGC TGCAGGTTGA CTATGAGATG CACAAGATGA CCCGGGACCC GCTGAACCTG 
GCGCTGCGCC GCTACGCGGG CATGACCCTC ACCAACCTCA CCTTTGGGGA CGTTGCCAAC 
AAGGCCACCC TGTGTGCGCG CCGCGGCTGC ATGGAGGCCA TCGTGGCCCA GCTGGCCTCC 
GACAGTGAGG AGCTCCACCA GGTGGTGTCC AGCATCCTTC GGAACTTGTC CTGGAGGGCC 
GACATCAACA GCAAGAAGGT GCTGAGGGAG GCGGGCAGCG TGACTGCCCT GGTGCAGTGT 
GTCCTGCGGG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TGAGCGCCCT GTGGAATCTG 
TCTGCACACA GCACAGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGCGC CCTGGGCTTC 
CTGGTGAGCA CCCTGACCTA CAAGTGTCAG AGCAACTCGC TGGCCATCAT CGAGAGCGGC 
GGCGGCATCC TCCGCAATGT GTCCAGCCTC GTCGCCACCC GTGAGGACTA CAGGCAGGTG 
CTCCGGGATC ACAACTGTCT GCAGACGCTG CTGCAGCATC TGACTTCGCA CAGCCTGACC 
ATCGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCGC CCGTGACCAG 
GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGTA ATCTGGTGCA CTCCAAGCAC 
AAGATGATCG CCATGGGCAG CGCCGCCGCC CTGCGCAACC TGCTGGCCCA TCGGCCCGCC 
AAGCACCAGG CGGCCGCCAC CGCCGTGTCC CCAGGCAGCT GCGTGCCCAG CCTGTACGTG 
CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACGCACGGC ACCTCGCGCA GGCGCTGGAG 
CACCTGGAGA AGCAGGGCCC GCCGGCAGCC GAGGCCGCCA CTAAGAAGCC GCTGCCGCCC 
CTGCGACACC TGGACGGCCT GGCCCAAGAC TATGCTTCCG ATTCGGGCTG CTTTGACGAC 
GACGATGCAC CGTCATCCCT GGCTGCGGCC GCGGCCACCG GGGAGCCAGC CAGCCCTGCC 
GCGCTGTCCC TCTTCCTGGG CAGCCCCTTC CTGCAGGGGC AGGCGCTGGC TCGCACCCCG 
CCCACCCGCC GAGGCGGCAA GGAGGCAGAG AAGGACACCA GTGGGGAGGC AGCCGTGGCG 
GCCAAGGCCA AGGCCAAGCT GGCGCTTGCA GTGGCGCGCA TCGACCAGCT GGTGGAGGAC 
ATCTCCGCCC TGCACACCTC GTCCGACGAT AGCTTCAGCC TCAGCTCTGG AGACCCGGGA 
CAGGAGGCGC CACGGGAGGG CCGCGCCCAG TCCTGCTCGC CATGCCGCGG CCCGGAGGGC 
GGGCGGCGAG AGGCAGGAAG CCGGGCGCAC CCGCTGCTGC GGCTCAAGGC GGCCCACGCC 
AGCCTCTCCA ACGACAGCCT CAACAGCGGC AGTGCCAGCG ACGGGTACTG CCCACGCGAA 
CATATGCTGC CCTGCCCGCT GGCCGCACTG GCTTCGCGCC GCGAGGACCC CAGGTGTGGG 
CAGCCTCGGC CCAGCCGGCT TGACCTTGAC CTGCCCGGCT GCCAGGCCGA GCCCCCGGCC 
CGCGAGGCCA CCTCCGCCGA CGCCCGCGTG CGCACCATCA AGCTGTCGCC TACCTATCAG 
CACGTGCCAC TGCTTGAGGG TGCCTCAAGG GCGGGTGCAG AGCCCCTCGC GGGGCCTGGA 
ATCTCTCCAG GGGCCCGGAA GCAGGCCTGG CTGCCGGCAG ACCACCTGAG CAAGGTTCCC 
GAGAAGCTGG CGGCTGCCCC GCTGTCTGTG GCCAGCAAGG CACTGCAGAA ACTGGCGGCG 
CAAGAGGGGC CACTCTCGCT GTCCCGATGC AGCTCCCTTT CCTCGCTGTC CTCGGCCGGC 
CGCCCAGGCC CCAGCGAGGG TGGTGACCTG GATGACAGTG ACTCCTCCCT GGAGGGGCTG 
GAGGAGGCCG GCCCCAGCGA GGCTGAGCTG GACAGCACGT GGCGGGCGCC CGGGGCCACC 
TCGCTGCCCG TAGCCATTCC GGCTCCCCGG CGTAACCGAG GCCGGGGCCT GGGGGTGGAA 
GACGCCACGC CGTCCAGCTC GTCGGAGAAC TACGTGCAGG AGACACCGCT TGTGCTGAGC 
CGCTGCAGCT CTGTGAGCTC GCTGGGCAGC TTCGAGAGCC CGTCCATCGC CAGCTCCATC 
CCCAGTGAAC CTTGCAGCGG GCAGGGCAGC GGCACCATCA GCCCTAGCGA GCTGCCCGAC 
AGCCCCGGAC AGACCATGCC TCCCAGCCGG AGCAAGACGC CACCGCTGGC GCCCGCGCCA 
CAGGGTCCCC CCGAGGCCAC CCAGTTCAGC CTGCAGTGGG AGAGCTACGT GAAGCGCTTC 
CTGGACATCG CCGACTGCCG GGAGCGCTGC CGGCTGCCAT CTGAGCTGGA CGCAGGCAGC 
GTGCGCTTTA CCGTGGAGAA GCCAGACGAG AACTTCTCGT GCGCCTCCAG CCTCAGCGCG 
CTGGCCTTGC ACGAGCACTA CGTGCAGCAG GACGTGGAGC TGCGGCTGCT GCCCTCGGCC 
TGCCCCGAGC GCGGCGGGGG CGCCGGGGGC GCCGGCCTCC ACTTTGCAGG GCACCGGCGG 
CGGGAGGAGG GGCCGGCGCC CACGGGTTCT CGCCCTCGCG GCGCCGCGGA CCAGGAGCTG 
GAACTGCTGC GGGAGTGCCT GGGAGCCGCC GTGCCTGCCC GGCTGCGCAA GGTGGCCTCC 
GCGCTGGTGC CAGGTCGCCG CGCACTCCCC GTGCCCGTCT ACATGTTGGT GCCCGCCCCG 
GCCCCGGCCC AGGAGGACGA CTCCTGCACT GACTCCGCGG AGGGCACGCC GGTCAACTTC 
TCTAGCGCCG CCTCGCTCAG CGACGAGACG CTGCAGGGAC CCCCCAGGGA CCAGCCCGGG 
GGACCAGCGG GCAGGCAAAG ACCCACCGGC CGCCCCACCT CTGCCAGACA GGCCATGGGG 
CACCGGCACA AGGCGGGAGG CGCCGGCCGC AGCGCGGAGC AGTCTCGGGG CGCGGGCAAG 
AACAGAGCAG GGCTGGAGCT GCCCCTGGGC CGGCCCCCGA GCGCCCCCGC AGACAAGGAC 
GGCTCAAAGC CCGGCCGGAC CCGCGGGGAC GGGGCGCTCC AGTCGCTGTG CCTCACGACG 
CCCACTGAGG AGGCCGTGTA CTGCTTCTAC GGCAACGACT CGGACGAGGA GCCCCCGGCG 
GCCGCGCCCA CGCCAACCCA CCGGCGCACA TCGGCCATCC CTCGCGCTTT TACGCGGGAG 
CGTCCGCAGG GCCGGAAGGA GGCCCCTGCC CCGTCCAAGG CTGCACCAGC TGCCCCGCCG 
CCCGCCCGGA CCCAGCCCAG CCTCATTGCT GACGAGACCC CGCCCTGCTA CTCCCTGAGC 
TCCTCCGCCA GCTCCCTCAG CGAGCCCGAG CCCTCGGAGC CGCCGGCCGT CCATCCACGA 
GGCCGGGAGC CCGCGGTCAC CAAGGACCCG GGCCCAGGAG GCGGACGCGA CAGCTCGCCC 
AGCCCGCGGG CCGCGGAGGA GCTTCTGCAG CGGTGCATCA GCTCGGCCCT GCCCAGGCGC 
CGGCCCCCCG TGTCTGGCCT GCGGCGCCGC AAGCCCCGAG CCACCCGGCT GGATGAGCGG 
CCCGCAGAGG GGTCCCGGGA ACGCGGCGAG GAGGCAGCGG GCTCGGACCG GGCCTCCGAC 
CTGGATAGCG TGGAGTGGCG CGCCATCCAG GAGGGCGCCA ATTCAATTGT CACGTGGCTG 
CACCAGGCAG CAGCTGCCAC GCGGGAGGCC TCGTCCGAGT CCGACTCCAT CCTGTCCTTC 
GTATCCGGGC TGTCAGTGGG ATCCACCCTA CAGCCCCCCA AGCACAGGAA GGGACGACAG 
GCGGAGGGAG AAATGGGCAG TGCCCGGCGG CCAGAGAAAA GGGGCGCAGC CTCAGTCAAG 
ACCAGCGGGA GCCCCCGTTC CCCTGCAGGC CCCGAGAAGC CACGTGGCAC ACAGAAGACC 
ACGCCCGGGG TGCCAGCTGT GCTCCGGGGA CGAACAGTGA TCTACGTCCC CAGCCCGGCA 
CCCCGTGCCC AGCCCAAAGG GACCCCCGGC CCCCGCGCCA CACCGCGGAA GGTGGCGCCC 
CCTTGCCTGG CACAGCCCGC GGCTCCAGCC AAAGTCCCGA GCCCCGGGCA GCAGCGGTCG 
CGGAGCCTAC ACCGGCCTGC CAAGACCTCG GAGCTGGCGA CGCTGAGCCA GCCCCCCAGA 
AGCGCCACAC CGCCCGCCCG CCTCGCCAAG ACCCCCTCCT CCAGCTCCTC CCAGACCTCG 
CCCGCCTCCC AGCCCCTGCC CAGAAAGCGC CCCCCGGTCA CCCAGGCTGC TGGGGCCCTG 
CCCGGCCCCG GAGCCTCCCC GGTGCCCAAA ACGCCGGCGC GCACCCTTCT GGCGAAGCAG 
CACAAGACGC AGAGATCGCC CGTGCGGATC CCGTTCATGC AGAGGCCGGC CCGGCGTGGG 
CCGCCACCGC TGGCTCGGGC AGTCCCGGAG CCGGGCCCCA GGGGCCGGGC GGGGACCGAG 
GCGGGCCCGG GGGCGCGCGG GGGCCGCCTG GGCCTGGTGC GTGTGGCCTC AGCCCTCTCC 
AGCGGCAGCG AGTCCTCCGA CCGCTCGGGC TTCCGGCGAC AGCTAACCTT CATCAAGGAG 
TCGCCGGGCT TGCGGCGCCG CCGCTCCGAG CTGTCCTCGG CCGAGTCCGC GGCCTCTGCC 
CCCCAGGGCG CCTCGCCCCG CCGCGGCCGG CCCGCGCTGC CCGCCGTCTT CCTCTGCTCC 
TCGCGCTGCG AAGAGCTCCG AGCGGCACCC CGGCAGGGCC CGGCCCCGGC CCGGCAGCGG 
CCCCCCGCGG CCCGACCCAG CCCTGGCGAG CGCCCTGCCC GGCGCACCAC CTCCGAGAGC 
CCGTCCCGCC TGCCTGTGCG CGCGCCCGCC GCCCGGCCGG AGACTGTCAA GCGCTACGCG 
TCGCTGCCGC ACATCAGCGT GGCCCGCAGG CCCGACGGCG CCGTCCCCGC GGCCCCTGCC 
TCAGCCGACG CCGCGCGCCG CAGCAGCGAC GGGGAGCCCC GGCCGCTCCC CAGGGTGGCC 
GCGCCGGGCA CGACCTGGCG GCGCATCCGA GATGAGGACG TGCCCCACAT CCTGCGCAGC 
ACGCTTCCCG CCACGGCCCT GCCACTGCGG GGCTCCACGC CCGAGGACGC CCCGGCCGGG 
CCCCCGCCGC GCAAGACCAG CGACGCCGTG GTCCAGACCG AGGAGGTCGC CGCCCCCAAG 
ACCAACTCCA GCACGTCCCC GAGCCTGGAG ACCAGGGAGC CCCCCGGGGC CCCCGCCGGC 
GGCCAGCTCT CCCTCCTCGG CAGCGACGTG GACGGTCCCA GCCTCGCCAA GGCTCCCATC 
TCCGCACCCT TCGTGCACGA GGGCCTGGGG GTCGCCGTGG GGGGCTTCCC CGCCAGCCGG 
CACGGCTCCC CCAGCCGCTC GGCCCGAGTA CCCCCCTTCA ACTATGTGCC CAGCCCCATG 
GTGGTCGCAG CCACCACCGA CTCGGCCGCG GAGAAAGCCC CGGCCACTGC CTCCGCCACC 
CTCCTGGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722673.2
CDS	50..7261
Translation	MAPARLPAPSLAVSPASLIPHPPPLAVVSPSARSGSAACPGPDRALSAPEPLPAPRRPRSPRAPRPPRAGISGGARGAAQREEAPDPGAPPAQLHTLQELKMASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQTEVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCFLLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQRAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLLSMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAALHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMKLSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGCMEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNLSAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTLLQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPAKHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQDYASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVAAKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAHPLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPAREATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSVASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGATSLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGSGTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGSVRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGSRPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNFSSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLGRPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRERPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDPGPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASDLDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARRPEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAPPCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKRPPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTEAGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGRPALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYASLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLRGSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPISAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE

Target ORF information:

RefSeq Version	XM_006722610.3
Organism	Homo sapiens(human)
Definition	Homo sapiens APC regulator of WNT signaling pathway 2 (APC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722610.3

ORF Insert Sequence:

ATGGCACCAG CCCGTCTTCC CGCGCCCTCA TTGGCTGTCT CACCCGCATC CCTCATCCCG 
CATCCTCCCC CGCTCGCGGT GGTCTCGCCC AGCGCTAGGA GCGGCAGCGC CGCCTGCCCA 
GGCCCGGACC GGGCTTTGTC CGCCCCGGAG CCCCTGCCCG CGCCGCGGAG ACCCCGGAGC 
CCGCGCGCTC CGAGGCCACC CCGGGCCGGG ATTTCCGGTG GGGCCCGCGG AGCCGCGCAG 
AGGGAGGAGG CCCCAGACCC AGGCGCCCCG CCAGCCCAGC TGCACACGCT GCAGGAGCTG 
AAGATGGCGA GCTCCGTGGC GCCCTACGAG CAGCTGGTGA GGCAGGTGGA GGCCTTGAAG 
GCTGAGAACA GCCACCTGAG GCAGGAGCTA AGGGACAACT CCAGCCACCT GTCCAAGCTG 
GAGACAGAGA CGTCGGGCAT GAAGGAGGTC CTGAAGCACC TACAGGGAAA ACTGGAGCAG 
GAGGCCCGAG TGCTGGTGTC CTCGGGGCAG ACGGAGGTGC TGGAGCAGCT GAAGGCCCTA 
CAGATGGACA TCACCAGCCT GTACAACCTC AAGTTCCAGC CGCCCACCCT GGGCCCGGAG 
CCTGCCGCCC GGACCCCCGA GGGCAGCCCA GTACACGGCT CCGGGCCCTC CAAGGACAGC 
TTTGGGGAGC TGAGCCGGGC CACCATCCGG CTGCTGGAGG AACTGGACCG GGAACGGTGT 
TTCCTGCTGA ATGAGATTGA GAAGGAGGAG AAGGAGAAGC TCTGGTACTA CTCTCAGCTG 
CAGGGCCTGT CCAAGCGCCT GGACGAGCTG CCGCACGTGG AGACGTTCTC GATGCAGATG 
GACCTGATCC GGCAGCAGCT TGAGTTCGAG GCCCAGCACA TCCGCTCGCT GATGGAGGAG 
CGCTTCGGCA CCTCGGACGA GATGGTGCAG CGGGCACAGA TCCGCGCCTC GCGCCTGGAG 
CAGATTGACA AGGAGCTGCT GGAGGCGCAG GACCGAGTGC AGCAGACGGA GCCCCAGGCC 
TTGCTGGCGG TGAAGTCGGT GCCGGTGGAC GAGGACCCCG AGACAGAGGT CCCCACACAC 
CCTGAGGATG GCACCCCTCA GCCGGGCAAC AGCAAGGTGG AGGTGGTCTT CTGGCTGTTG 
TCCATGTTGG CGACGCGCGA CCAGGAGGAT ACAGCGCGCA CGCTGCTGGC CATGTCCAGC 
TCGCCCGAGA GCTGCGTGGC CATGCGCCGC TCGGGCTGTC TGCCTCTGCT GCTGCAAATC 
CTCCACGGCA CCGAGGCCGC GGCCGGGGGT CGCGCCGGGG CCCCAGGGGC ACCGGGCGCC 
AAGGACGCAC GCATGCGCGC CAACGCGGCG CTGCACAACA TCGTCTTCTC GCAGCCGGAC 
CAGGGCCTGG CGCGCAAGGA GATGCGCGTC CTGCACGTGC TGGAGCAGAT CCGGGCCTAC 
TGCGAGACCT GCTGGGACTG GCTGCAGGCC CGAGACGGCG GGCCCGAGGG AGGTGGCGCC 
GGCAGCGCCC CGATCCCCAT CGAGCCGCAG ATCTGCCAGG CCACCTGTGC TGTTATGAAG 
CTGTCCTTTG ATGAGGAGTA CCGCCGTGCC ATGAACGAGC TAGGTGGGCT GCAGGCCGTG 
GCAGAGCTGC TGCAGGTTGA CTATGAGATG CACAAGATGA CCCGGGACCC GCTGAACCTG 
GCGCTGCGCC GCTACGCGGG CATGACCCTC ACCAACCTCA CCTTTGGGGA CGTTGCCAAC 
AAGGCCACCC TGTGTGCGCG CCGCGGCTGC ATGGAGGCCA TCGTGGCCCA GCTGGCCTCC 
GACAGTGAGG AGCTCCACCA GGTGGTGTCC AGCATCCTTC GGAACTTGTC CTGGAGGGCC 
GACATCAACA GCAAGAAGGT GCTGAGGGAG GCGGGCAGCG TGACTGCCCT GGTGCAGTGT 
GTCCTGCGGG CCACCAAGGA GTCCACCCTG AAGAGCGTGC TGAGCGCCCT GTGGAATCTG 
TCTGCACACA GCACAGAGAA CAAGGCGGCC ATCTGCCAGG TGGATGGCGC CCTGGGCTTC 
CTGGTGAGCA CCCTGACCTA CAAGTGTCAG AGCAACTCGC TGGCCATCAT CGAGAGCGGC 
GGCGGCATCC TCCGCAATGT GTCCAGCCTC GTCGCCACCC GTGAGGACTA CAGGCAGGTG 
CTCCGGGATC ACAACTGTCT GCAGACGCTG CTGCAGCATC TGACTTCGCA CAGCCTGACC 
ATCGTGAGCA ACGCGTGCGG CACGCTCTGG AACCTGTCGG CCCGCAGCGC CCGTGACCAG 
GAGCTGCTGT GGGACCTGGG CGCCGTGGGC ATGCTGCGTA ATCTGGTGCA CTCCAAGCAC 
AAGATGATCG CCATGGGCAG CGCCGCCGCC CTGCGCAACC TGCTGGCCCA TCGGCCCGCC 
AAGCACCAGG CGGCCGCCAC CGCCGTGTCC CCAGGCAGCT GCGTGCCCAG CCTGTACGTG 
CGCAAGCAGC GGGCGCTGGA GGCCGAGCTG GACGCACGGC ACCTCGCGCA GGCGCTGGAG 
CACCTGGAGA AGCAGGGCCC GCCGGCAGCC GAGGCCGCCA CTAAGAAGCC GCTGCCGCCC 
CTGCGACACC TGGACGGCCT GGCCCAAGAC TATGCTTCCG ATTCGGGCTG CTTTGACGAC 
GACGATGCAC CGTCATCCCT GGCTGCGGCC GCGGCCACCG GGGAGCCAGC CAGCCCTGCC 
GCGCTGTCCC TCTTCCTGGG CAGCCCCTTC CTGCAGGGGC AGGCGCTGGC TCGCACCCCG 
CCCACCCGCC GAGGCGGCAA GGAGGCAGAG AAGGACACCA GTGGGGAGGC AGCCGTGGCG 
GCCAAGGCCA AGGCCAAGCT GGCGCTTGCA GTGGCGCGCA TCGACCAGCT GGTGGAGGAC 
ATCTCCGCCC TGCACACCTC GTCCGACGAT AGCTTCAGCC TCAGCTCTGG AGACCCGGGA 
CAGGAGGCGC CACGGGAGGG CCGCGCCCAG TCCTGCTCGC CATGCCGCGG CCCGGAGGGC 
GGGCGGCGAG AGGCAGGAAG CCGGGCGCAC CCGCTGCTGC GGCTCAAGGC GGCCCACGCC 
AGCCTCTCCA ACGACAGCCT CAACAGCGGC AGTGCCAGCG ACGGGTACTG CCCACGCGAA 
CATATGCTGC CCTGCCCGCT GGCCGCACTG GCTTCGCGCC GCGAGGACCC CAGGTGTGGG 
CAGCCTCGGC CCAGCCGGCT TGACCTTGAC CTGCCCGGCT GCCAGGCCGA GCCCCCGGCC 
CGCGAGGCCA CCTCCGCCGA CGCCCGCGTG CGCACCATCA AGCTGTCGCC TACCTATCAG 
CACGTGCCAC TGCTTGAGGG TGCCTCAAGG GCGGGTGCAG AGCCCCTCGC GGGGCCTGGA 
ATCTCTCCAG GGGCCCGGAA GCAGGCCTGG CTGCCGGCAG ACCACCTGAG CAAGGTTCCC 
GAGAAGCTGG CGGCTGCCCC GCTGTCTGTG GCCAGCAAGG CACTGCAGAA ACTGGCGGCG 
CAAGAGGGGC CACTCTCGCT GTCCCGATGC AGCTCCCTTT CCTCGCTGTC CTCGGCCGGC 
CGCCCAGGCC CCAGCGAGGG TGGTGACCTG GATGACAGTG ACTCCTCCCT GGAGGGGCTG 
GAGGAGGCCG GCCCCAGCGA GGCTGAGCTG GACAGCACGT GGCGGGCGCC CGGGGCCACC 
TCGCTGCCCG TAGCCATTCC GGCTCCCCGG CGTAACCGAG GCCGGGGCCT GGGGGTGGAA 
GACGCCACGC CGTCCAGCTC GTCGGAGAAC TACGTGCAGG AGACACCGCT TGTGCTGAGC 
CGCTGCAGCT CTGTGAGCTC GCTGGGCAGC TTCGAGAGCC CGTCCATCGC CAGCTCCATC 
CCCAGTGAAC CTTGCAGCGG GCAGGGCAGC GGCACCATCA GCCCTAGCGA GCTGCCCGAC 
AGCCCCGGAC AGACCATGCC TCCCAGCCGG AGCAAGACGC CACCGCTGGC GCCCGCGCCA 
CAGGGTCCCC CCGAGGCCAC CCAGTTCAGC CTGCAGTGGG AGAGCTACGT GAAGCGCTTC 
CTGGACATCG CCGACTGCCG GGAGCGCTGC CGGCTGCCAT CTGAGCTGGA CGCAGGCAGC 
GTGCGCTTTA CCGTGGAGAA GCCAGACGAG AACTTCTCGT GCGCCTCCAG CCTCAGCGCG 
CTGGCCTTGC ACGAGCACTA CGTGCAGCAG GACGTGGAGC TGCGGCTGCT GCCCTCGGCC 
TGCCCCGAGC GCGGCGGGGG CGCCGGGGGC GCCGGCCTCC ACTTTGCAGG GCACCGGCGG 
CGGGAGGAGG GGCCGGCGCC CACGGGTTCT CGCCCTCGCG GCGCCGCGGA CCAGGAGCTG 
GAACTGCTGC GGGAGTGCCT GGGAGCCGCC GTGCCTGCCC GGCTGCGCAA GGTGGCCTCC 
GCGCTGGTGC CAGGTCGCCG CGCACTCCCC GTGCCCGTCT ACATGTTGGT GCCCGCCCCG 
GCCCCGGCCC AGGAGGACGA CTCCTGCACT GACTCCGCGG AGGGCACGCC GGTCAACTTC 
TCTAGCGCCG CCTCGCTCAG CGACGAGACG CTGCAGGGAC CCCCCAGGGA CCAGCCCGGG 
GGACCAGCGG GCAGGCAAAG ACCCACCGGC CGCCCCACCT CTGCCAGACA GGCCATGGGG 
CACCGGCACA AGGCGGGAGG CGCCGGCCGC AGCGCGGAGC AGTCTCGGGG CGCGGGCAAG 
AACAGAGCAG GGCTGGAGCT GCCCCTGGGC CGGCCCCCGA GCGCCCCCGC AGACAAGGAC 
GGCTCAAAGC CCGGCCGGAC CCGCGGGGAC GGGGCGCTCC AGTCGCTGTG CCTCACGACG 
CCCACTGAGG AGGCCGTGTA CTGCTTCTAC GGCAACGACT CGGACGAGGA GCCCCCGGCG 
GCCGCGCCCA CGCCAACCCA CCGGCGCACA TCGGCCATCC CTCGCGCTTT TACGCGGGAG 
CGTCCGCAGG GCCGGAAGGA GGCCCCTGCC CCGTCCAAGG CTGCACCAGC TGCCCCGCCG 
CCCGCCCGGA CCCAGCCCAG CCTCATTGCT GACGAGACCC CGCCCTGCTA CTCCCTGAGC 
TCCTCCGCCA GCTCCCTCAG CGAGCCCGAG CCCTCGGAGC CGCCGGCCGT CCATCCACGA 
GGCCGGGAGC CCGCGGTCAC CAAGGACCCG GGCCCAGGAG GCGGACGCGA CAGCTCGCCC 
AGCCCGCGGG CCGCGGAGGA GCTTCTGCAG CGGTGCATCA GCTCGGCCCT GCCCAGGCGC 
CGGCCCCCCG TGTCTGGCCT GCGGCGCCGC AAGCCCCGAG CCACCCGGCT GGATGAGCGG 
CCCGCAGAGG GGTCCCGGGA ACGCGGCGAG GAGGCAGCGG GCTCGGACCG GGCCTCCGAC 
CTGGATAGCG TGGAGTGGCG CGCCATCCAG GAGGGCGCCA ATTCAATTGT CACGTGGCTG 
CACCAGGCAG CAGCTGCCAC GCGGGAGGCC TCGTCCGAGT CCGACTCCAT CCTGTCCTTC 
GTATCCGGGC TGTCAGTGGG ATCCACCCTA CAGCCCCCCA AGCACAGGAA GGGACGACAG 
GCGGAGGGAG AAATGGGCAG TGCCCGGCGG CCAGAGAAAA GGGGCGCAGC CTCAGTCAAG 
ACCAGCGGGA GCCCCCGTTC CCCTGCAGGC CCCGAGAAGC CACGTGGCAC ACAGAAGACC 
ACGCCCGGGG TGCCAGCTGT GCTCCGGGGA CGAACAGTGA TCTACGTCCC CAGCCCGGCA 
CCCCGTGCCC AGCCCAAAGG GACCCCCGGC CCCCGCGCCA CACCGCGGAA GGTGGCGCCC 
CCTTGCCTGG CACAGCCCGC GGCTCCAGCC AAAGTCCCGA GCCCCGGGCA GCAGCGGTCG 
CGGAGCCTAC ACCGGCCTGC CAAGACCTCG GAGCTGGCGA CGCTGAGCCA GCCCCCCAGA 
AGCGCCACAC CGCCCGCCCG CCTCGCCAAG ACCCCCTCCT CCAGCTCCTC CCAGACCTCG 
CCCGCCTCCC AGCCCCTGCC CAGAAAGCGC CCCCCGGTCA CCCAGGCTGC TGGGGCCCTG 
CCCGGCCCCG GAGCCTCCCC GGTGCCCAAA ACGCCGGCGC GCACCCTTCT GGCGAAGCAG 
CACAAGACGC AGAGATCGCC CGTGCGGATC CCGTTCATGC AGAGGCCGGC CCGGCGTGGG 
CCGCCACCGC TGGCTCGGGC AGTCCCGGAG CCGGGCCCCA GGGGCCGGGC GGGGACCGAG 
GCGGGCCCGG GGGCGCGCGG GGGCCGCCTG GGCCTGGTGC GTGTGGCCTC AGCCCTCTCC 
AGCGGCAGCG AGTCCTCCGA CCGCTCGGGC TTCCGGCGAC AGCTAACCTT CATCAAGGAG 
TCGCCGGGCT TGCGGCGCCG CCGCTCCGAG CTGTCCTCGG CCGAGTCCGC GGCCTCTGCC 
CCCCAGGGCG CCTCGCCCCG CCGCGGCCGG CCCGCGCTGC CCGCCGTCTT CCTCTGCTCC 
TCGCGCTGCG AAGAGCTCCG AGCGGCACCC CGGCAGGGCC CGGCCCCGGC CCGGCAGCGG 
CCCCCCGCGG CCCGACCCAG CCCTGGCGAG CGCCCTGCCC GGCGCACCAC CTCCGAGAGC 
CCGTCCCGCC TGCCTGTGCG CGCGCCCGCC GCCCGGCCGG AGACTGTCAA GCGCTACGCG 
TCGCTGCCGC ACATCAGCGT GGCCCGCAGG CCCGACGGCG CCGTCCCCGC GGCCCCTGCC 
TCAGCCGACG CCGCGCGCCG CAGCAGCGAC GGGGAGCCCC GGCCGCTCCC CAGGGTGGCC 
GCGCCGGGCA CGACCTGGCG GCGCATCCGA GATGAGGACG TGCCCCACAT CCTGCGCAGC 
ACGCTTCCCG CCACGGCCCT GCCACTGCGG GGCTCCACGC CCGAGGACGC CCCGGCCGGG 
CCCCCGCCGC GCAAGACCAG CGACGCCGTG GTCCAGACCG AGGAGGTCGC CGCCCCCAAG 
ACCAACTCCA GCACGTCCCC GAGCCTGGAG ACCAGGGAGC CCCCCGGGGC CCCCGCCGGC 
GGCCAGCTCT CCCTCCTCGG CAGCGACGTG GACGGTCCCA GCCTCGCCAA GGCTCCCATC 
TCCGCACCCT TCGTGCACGA GGGCCTGGGG GTCGCCGTGG GGGGCTTCCC CGCCAGCCGG 
CACGGCTCCC CCAGCCGCTC GGCCCGAGTA CCCCCCTTCA ACTATGTGCC CAGCCCCATG 
GTGGTCGCAG CCACCACCGA CTCGGCCGCG GAGAAAGCCC CGGCCACTGC CTCCGCCACC 
CTCCTGGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hsa-miR-11181 regulates Wnt signaling pathway through targeting of APC2 transcripts in SW480 cell line.
Gene641297-302(2018 Jan)
Dokanehiifard S,Soltani BM

Familial adenomatous polyposis in children younger than age ten years: a multidisciplinary clinic experience.
Diseases of the colon and rectum51(2)207-12(2008 Feb)
Attard TM,Tajouri T,Peterson KD,Tinley S,Thorson AG,Lynch HT

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APC2 cDNA ORF clone, Homo sapiens(human)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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