PRG4 cDNA ORF clone, Homo sapiens(human)

The following PRG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu63780 NM_005807.6
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant A, also known as V0, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu34060 NM_001303232.2
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu63778 NM_001127709.3
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu63779 NM_001127708.3
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu63781 NM_001127710.3
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu63778 XM_017000002.1
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu63779 XM_024448717.1
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu63780 XM_024448707.1
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu63781 XM_017000003.1
Latest version!
Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OHu34060 NM_001303232.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu63781 NM_001127710.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu63778 NM_001127709.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu63780 NM_005807.4 Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu63780 NM_005807.5 Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu63779 NM_001127708.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu63780
    Clone ID Related Accession (Same CDS sequence) NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
    Accession Version NM_005807.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-29
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform A preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133553.9 and KF455089.1. This sequence is a reference standard in the RefSeqGene project. On Oct 29, 2019 this sequence version replaced NM_005807.5. Transcript Variant: This variant (A, also known as V0) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70136.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005798.3
    CDS46..4260
    Translation

    Target ORF information:

    RefSeq Version NM_005807.6
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant A, also known as V0, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005807.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34060
    Clone ID Related Accession (Same CDS sequence) NM_001303232.1 , NM_001303232.2
    Accession Version NM_001303232.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform E preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA995681.1, U70136.1, AL133553.9 and CA435357.1. On Dec 23, 2014 this sequence version replaced XM_005244822.2. Transcript Variant: This variant (E, also known as V1) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform E) is shorter than isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11518279, 14976050 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC
    AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA
    AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC
    AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT
    CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA
    GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG
    ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC
    ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT
    CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC
    AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT
    GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT
    CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA
    CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG
    TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC
    ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG
    CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC
    ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG
    CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG
    ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT
    ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC
    ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT
    AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT
    CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC
    AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA
    CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT
    CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC
    TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT
    ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC
    ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC
    AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA
    ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG
    CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG
    AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC
    CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA
    AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA
    ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT
    TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC
    AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT
    GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC
    AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC
    TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA
    AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA
    GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG
    AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA
    AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC
    TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC
    AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG
    AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC
    GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA
    ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001290161.1
    CDS52..4137
    Translation

    Target ORF information:

    RefSeq Version NM_001303232.1
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001303232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC
    AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA
    AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC
    AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT
    CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA
    GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG
    ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC
    ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT
    CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC
    AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT
    GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT
    CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA
    CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG
    TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC
    ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG
    CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC
    ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG
    CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG
    ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT
    ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC
    ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT
    AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT
    CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC
    AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA
    CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT
    CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC
    TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT
    ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC
    ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC
    AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA
    ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG
    CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG
    AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC
    CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA
    AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA
    ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT
    TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC
    AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT
    GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC
    AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC
    TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA
    AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA
    GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG
    AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA
    AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC
    TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC
    AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG
    AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC
    GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA
    ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34060
    Clone ID Related Accession (Same CDS sequence) NM_001303232.1 , NM_001303232.2
    Accession Version NM_001303232.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform E preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA995681.1, U70136.1, AL133553.9 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001303232.1. Transcript Variant: This variant (E, also known as V1) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform E) is shorter than isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11518279, 14976050 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC
    AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA
    AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC
    AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT
    CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA
    GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG
    ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC
    ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT
    CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC
    AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT
    GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT
    CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA
    CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG
    TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC
    ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG
    CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC
    ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG
    CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG
    ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT
    ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC
    ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT
    AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT
    CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC
    AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA
    CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT
    CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC
    TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT
    ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC
    ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC
    AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA
    ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG
    CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG
    AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC
    CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA
    AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA
    ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT
    TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC
    AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT
    GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC
    AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC
    TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA
    AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA
    GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG
    AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA
    AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC
    TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC
    AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG
    AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC
    GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA
    ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001290161.1
    CDS52..4137
    Translation

    Target ORF information:

    RefSeq Version NM_001303232.2
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001303232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC
    AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA
    AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC
    AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT
    CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA
    GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG
    ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC
    ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT
    CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC
    AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT
    GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT
    CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA
    CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG
    TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG
    CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC
    ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG
    CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC
    ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG
    CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG
    ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT
    ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC
    ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT
    AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT
    CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC
    AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA
    CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT
    CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC
    TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT
    ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC
    ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC
    AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA
    ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG
    CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG
    AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC
    CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA
    AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA
    ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT
    TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC
    AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT
    GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC
    AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC
    TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA
    AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA
    GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG
    AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA
    AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC
    TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC
    AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG
    AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC
    GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA
    ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT
    CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63778
    Clone ID Related Accession (Same CDS sequence) XM_017000002.1 , NM_001127709.2 , NM_001127709.3
    Accession Version NM_001127709.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform C preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC404857.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Dec 23, 2014 this sequence version replaced NM_001127709.1. Transcript Variant: This variant (C, also known as V2) lacks two alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform C) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC
    CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC
    ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA
    ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG
    ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT
    AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA
    GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC
    AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC
    ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT
    ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT
    ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA
    CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC
    AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA
    CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT
    GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT
    CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT
    CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT
    GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC
    CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT
    CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC
    ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG
    CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC
    ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT
    GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC
    AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA
    ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT
    GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA
    ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT
    ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT
    ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT
    TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT
    ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC
    AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC
    CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG
    AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG
    TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT
    ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA
    CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA
    AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC
    CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT
    TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC
    AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG
    AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT
    AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT
    GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA
    CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT
    CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT
    ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT
    CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG
    CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121181.2
    CDS52..3987
    Translation

    Target ORF information:

    RefSeq Version NM_001127709.2
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127709.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC
    CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC
    ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA
    ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG
    ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT
    AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA
    GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC
    AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC
    ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT
    ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT
    ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA
    CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC
    AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA
    CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT
    GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT
    CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT
    CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT
    GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC
    CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT
    CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC
    ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG
    CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC
    ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT
    GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC
    AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA
    ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT
    GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA
    ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT
    ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT
    ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT
    TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT
    ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC
    AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC
    CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG
    AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG
    TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT
    ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA
    CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA
    AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC
    CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT
    TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC
    AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG
    AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT
    AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT
    GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA
    CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT
    CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT
    ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT
    CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG
    CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63778
    Clone ID Related Accession (Same CDS sequence) XM_017000002.1 , NM_001127709.2 , NM_001127709.3
    Accession Version NM_001127709.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-29
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform C preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC404857.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001127709.2. Transcript Variant: This variant (C, also known as V2) lacks two alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform C) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC
    CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC
    ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA
    ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG
    ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT
    AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA
    GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC
    AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC
    ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT
    ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT
    ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA
    CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC
    AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA
    CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT
    GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT
    CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT
    CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT
    GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC
    CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT
    CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC
    ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG
    CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC
    ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT
    GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC
    AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA
    ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT
    GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA
    ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT
    ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT
    ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT
    TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT
    ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC
    AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC
    CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG
    AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG
    TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT
    ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA
    CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA
    AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC
    CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT
    TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC
    AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG
    AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT
    AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT
    GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA
    CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT
    CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT
    ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT
    CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG
    CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121181.2
    CDS52..3987
    Translation

    Target ORF information:

    RefSeq Version NM_001127709.3
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127709.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC
    CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC
    ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA
    ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG
    ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT
    AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA
    GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC
    AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC
    ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT
    ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT
    ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA
    CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC
    AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA
    CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT
    GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT
    CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT
    CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT
    GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC
    CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT
    CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC
    ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG
    CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC
    ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT
    GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC
    AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA
    ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT
    GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA
    ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT
    ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT
    ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT
    TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT
    ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC
    AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC
    CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG
    AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG
    TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT
    ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA
    CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA
    AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC
    CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT
    TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC
    AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG
    AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT
    AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT
    GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA
    CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT
    CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT
    ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT
    CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG
    CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63779
    Clone ID Related Accession (Same CDS sequence) XM_024448717.1 , NM_001127708.2 , NM_001127708.3
    Accession Version NM_001127708.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform B preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, AK131434.1, AL133553.9, KF455089.1, U70136.1 and CA435357.1. On Dec 23, 2014 this sequence version replaced NM_001127708.1. Transcript Variant: This variant (B, also known as V0a) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform B) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA
    CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC
    AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA
    ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA
    GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA
    GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC
    CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA
    CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG
    ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA
    GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA
    GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC
    CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC
    ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG
    CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT
    ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA
    CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC
    AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA
    CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT
    GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA
    CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC
    ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC
    CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT
    GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG
    CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT
    ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG
    CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT
    ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT
    GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT
    AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA
    AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA
    GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA
    TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT
    GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT
    GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT
    CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT
    AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA
    TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA
    ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA
    GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC
    ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT
    GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA
    TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA
    GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG
    TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT
    GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA
    CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT
    TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC
    CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA
    CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA
    CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA
    CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA
    CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT
    AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC
    AACTGTCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121180.2
    CDS52..4143
    Translation

    Target ORF information:

    RefSeq Version NM_001127708.2
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127708.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA
    CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC
    AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA
    ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA
    GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA
    GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC
    CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA
    CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG
    ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA
    GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA
    GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC
    CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC
    ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG
    CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT
    ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA
    CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC
    AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA
    CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT
    GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA
    CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC
    ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC
    CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT
    GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG
    CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT
    ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG
    CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT
    ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT
    GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT
    AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA
    AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA
    GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA
    TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT
    GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT
    GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT
    CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT
    AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA
    TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA
    ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA
    GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC
    ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT
    GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA
    TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA
    GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG
    TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT
    GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA
    CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT
    TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC
    CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA
    CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA
    CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA
    CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA
    CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT
    AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC
    AACTGTCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63779
    Clone ID Related Accession (Same CDS sequence) XM_024448717.1 , NM_001127708.2 , NM_001127708.3
    Accession Version NM_001127708.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform B preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, AK131434.1, AL133553.9, KF455089.1, U70136.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001127708.2. Transcript Variant: This variant (B, also known as V0a) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform B) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA
    CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC
    AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA
    ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA
    GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA
    GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC
    CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA
    CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG
    ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA
    GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA
    GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC
    CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC
    ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG
    CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT
    ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA
    CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC
    AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA
    CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT
    GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA
    CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC
    ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC
    CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT
    GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG
    CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT
    ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG
    CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT
    ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT
    GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT
    AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA
    AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA
    GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA
    TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT
    GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT
    GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT
    CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT
    AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA
    TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA
    ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA
    GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC
    ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT
    GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA
    TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA
    GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG
    TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT
    GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA
    CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT
    TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC
    CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA
    CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA
    CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA
    CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA
    CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT
    AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC
    AACTGTCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121180.2
    CDS52..4143
    Translation

    Target ORF information:

    RefSeq Version NM_001127708.3
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127708.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA
    CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC
    AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA
    ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA
    GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA
    GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC
    CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA
    CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG
    ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA
    GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA
    GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC
    CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC
    ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG
    CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT
    ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA
    CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC
    AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA
    CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT
    GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA
    CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC
    ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC
    CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT
    GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG
    CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT
    ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG
    CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT
    ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT
    GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT
    AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA
    AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA
    GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA
    TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT
    GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT
    GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT
    CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT
    AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA
    TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA
    ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA
    GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC
    ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT
    GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA
    TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA
    GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG
    TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT
    GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA
    CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT
    TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC
    CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA
    CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA
    CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA
    CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA
    CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT
    AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC
    AACTGTCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63780
    Clone ID Related Accession (Same CDS sequence) NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
    Accession Version NM_005807.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform A preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, U70136.1, AK131434.1, AL133553.9, KF455089.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_005807.4. Transcript Variant: This variant (A, also known as V0) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70136.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005798.3
    CDS52..4266
    Translation

    Target ORF information:

    RefSeq Version NM_005807.5
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005807.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63780
    Clone ID Related Accession (Same CDS sequence) NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
    Accession Version NM_005807.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform A preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, U70136.1, AK131434.1, AL133553.9, KF455089.1 and CA435357.1. This sequence is a reference standard in the RefSeqGene project. On Dec 23, 2014 this sequence version replaced NM_005807.3. Transcript Variant: This variant (A, also known as V0) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70136.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005798.3
    CDS52..4266
    Translation

    Target ORF information:

    RefSeq Version NM_005807.4
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005807.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63781
    Clone ID Related Accession (Same CDS sequence) XM_017000003.1 , NM_001127710.2 , NM_001127710.3
    Accession Version NM_001127710.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform D preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC295855.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Dec 23, 2014 this sequence version replaced NM_001127710.1. Transcript Variant: This variant (D, also known as V3) lacks three alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform D) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC
    AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA
    ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA
    GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG
    TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA
    CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG
    AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA
    GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT
    CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC
    AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC
    ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG
    GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC
    ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG
    GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC
    AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT
    AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC
    AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC
    TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT
    GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT
    TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT
    GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG
    GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA
    GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA
    CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA
    GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT
    ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC
    ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT
    AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC
    AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG
    ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA
    ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT
    GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC
    ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC
    ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG
    ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT
    CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC
    ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT
    CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA
    GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC
    TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA
    CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA
    GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC
    ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT
    AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA
    TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA
    TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG
    ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121182.2
    CDS52..3864
    Translation

    Target ORF information:

    RefSeq Version NM_001127710.2
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127710.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC
    AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA
    ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA
    GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG
    TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA
    CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG
    AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA
    GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT
    CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC
    AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC
    ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG
    GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC
    ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG
    GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC
    AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT
    AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC
    AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC
    TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT
    GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT
    TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT
    GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG
    GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA
    GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA
    CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA
    GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT
    ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC
    ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT
    AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC
    AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG
    ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA
    ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT
    GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC
    ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC
    ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG
    ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT
    CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC
    ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT
    CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA
    GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC
    TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA
    CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA
    GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC
    ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT
    AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA
    TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA
    TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG
    ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63781
    Clone ID Related Accession (Same CDS sequence) XM_017000003.1 , NM_001127710.2 , NM_001127710.3
    Accession Version NM_001127710.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform D preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC295855.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001127710.2. Transcript Variant: This variant (D, also known as V3) lacks three alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform D) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC
    AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA
    ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA
    GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG
    TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA
    CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG
    AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA
    GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT
    CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC
    AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC
    ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG
    GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC
    ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG
    GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC
    AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT
    AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC
    AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC
    TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT
    GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT
    TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT
    GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG
    GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA
    GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA
    CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA
    GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT
    ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC
    ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT
    AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC
    AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG
    ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA
    ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT
    GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC
    ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC
    ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG
    ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT
    CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC
    ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT
    CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA
    GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC
    TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA
    CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA
    GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC
    ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT
    AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA
    TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA
    TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG
    ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121182.2
    CDS52..3864
    Translation

    Target ORF information:

    RefSeq Version NM_001127710.3
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127710.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC
    AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA
    ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA
    GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG
    TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA
    CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG
    AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA
    GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT
    CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC
    AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC
    ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG
    GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC
    ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG
    GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC
    AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT
    AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC
    AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC
    TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT
    GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT
    TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT
    GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG
    GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA
    GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA
    CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA
    GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT
    ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC
    ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT
    AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC
    AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG
    ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA
    ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT
    GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC
    ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC
    ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG
    ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT
    CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC
    ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT
    CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA
    GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC
    TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA
    CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA
    GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC
    ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT
    AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA
    TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA
    TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG
    ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63778
    Clone ID Related Accession (Same CDS sequence) XM_017000002.1 , NM_001127709.2 , NM_001127709.3
    Accession Version XM_017000002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC
    CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC
    ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA
    ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG
    ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT
    AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA
    GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC
    AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC
    ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT
    ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT
    ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA
    CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC
    AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA
    CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT
    GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT
    CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT
    CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT
    GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC
    CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT
    CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC
    ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG
    CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC
    ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT
    GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC
    AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA
    ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT
    GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA
    ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT
    ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT
    ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT
    TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT
    ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC
    AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC
    CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG
    AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG
    TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT
    ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA
    CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA
    AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC
    CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT
    TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC
    AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG
    AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT
    AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT
    GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA
    CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT
    CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT
    ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT
    CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG
    CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855491.1
    CDS348..4283
    Translation

    Target ORF information:

    RefSeq Version XM_017000002.1
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC
    CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC
    ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA
    ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG
    ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT
    AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA
    GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC
    AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC
    ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC
    AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT
    ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG
    CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT
    ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA
    CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA
    CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC
    AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA
    CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT
    GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT
    CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT
    CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT
    GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC
    CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT
    CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC
    ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG
    CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC
    ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT
    GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC
    AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA
    ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT
    GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA
    ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT
    ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT
    ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT
    TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT
    ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC
    AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC
    CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG
    AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG
    TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT
    ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA
    CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA
    AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC
    CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT
    TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC
    AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG
    AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT
    AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT
    GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA
    CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT
    CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT
    ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT
    CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG
    CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63779
    Clone ID Related Accession (Same CDS sequence) XM_024448717.1 , NM_001127708.2 , NM_001127708.3
    Accession Version XM_024448717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA
    CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC
    AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA
    ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA
    GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA
    GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC
    CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA
    CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG
    ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA
    GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA
    GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC
    CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC
    ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG
    CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT
    ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA
    CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC
    AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA
    CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT
    GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA
    CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC
    ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC
    CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT
    GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG
    CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT
    ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG
    CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT
    ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT
    GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT
    AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA
    AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA
    GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA
    TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT
    GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT
    GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT
    CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT
    AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA
    TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA
    ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA
    GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC
    ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT
    GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA
    TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA
    GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG
    TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT
    GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA
    CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT
    TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC
    CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA
    CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA
    CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA
    CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA
    CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT
    AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC
    AACTGTCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304485.1
    CDS38..4129
    Translation

    Target ORF information:

    RefSeq Version XM_024448717.1
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA
    CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC
    AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA
    ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA
    GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA
    GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC
    CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA
    CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG
    ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA
    GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA
    GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC
    CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC
    ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG
    CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT
    ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC
    AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA
    CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC
    AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA
    CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT
    GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT
    AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA
    CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC
    ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC
    CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT
    GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG
    CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT
    ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG
    CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT
    ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT
    GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT
    AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA
    AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA
    GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA
    TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT
    GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT
    GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT
    CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT
    AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA
    TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA
    ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA
    GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC
    ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT
    GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA
    TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA
    GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG
    TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT
    GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA
    CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT
    TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC
    CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA
    CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA
    CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA
    CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA
    CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT
    AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC
    AACTGTCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63780
    Clone ID Related Accession (Same CDS sequence) NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
    Accession Version XM_024448707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304475.1
    CDS38..4252
    Translation

    Target ORF information:

    RefSeq Version XM_024448707.1
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA
    GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC
    AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA
    GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT
    GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT
    CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA
    AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT
    TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG
    AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA
    AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT
    GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC
    ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC
    AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA
    GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA
    AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT
    TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA
    GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT
    ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC
    ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC
    AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA
    CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA
    GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC
    ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC
    ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC
    CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC
    GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC
    CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT
    GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC
    CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT
    GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG
    CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT
    ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG
    ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG
    GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA
    ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG
    AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA
    ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT
    TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA
    ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA
    GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG
    ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA
    ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT
    CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA
    CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA
    CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG
    CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA
    GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC
    CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT
    GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC
    GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC
    GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG
    ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT
    CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT
    AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC
    AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT
    GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT
    TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG
    TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG
    AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT
    TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT
    ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA
    AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG
    CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG
    TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63781
    Clone ID Related Accession (Same CDS sequence) XM_017000003.1 , NM_001127710.2 , NM_001127710.3
    Accession Version XM_017000003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product proteoglycan 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC
    AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA
    ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA
    GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG
    TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA
    CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG
    AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA
    GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT
    CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC
    AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC
    ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG
    GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC
    ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG
    GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC
    AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT
    AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC
    AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC
    TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT
    GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT
    TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT
    GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG
    GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA
    GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA
    CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA
    GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT
    ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC
    ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT
    AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC
    AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG
    ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA
    ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT
    GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC
    ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC
    ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG
    ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT
    CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC
    ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT
    CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA
    GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC
    TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA
    CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA
    GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC
    ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT
    AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA
    TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA
    TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG
    ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016855492.1
    CDS38..3850
    Translation

    Target ORF information:

    RefSeq Version XM_017000003.1
    Organism Homo sapiens(human)
    Definition Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017000003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
    CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG
    AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG
    AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC
    AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA
    ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA
    GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG
    TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA
    CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG
    AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA
    GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT
    CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG
    GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC
    ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT
    GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC
    CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT
    TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC
    AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC
    ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC
    ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG
    GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC
    ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG
    GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC
    AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG
    GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA
    ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA
    CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT
    AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT
    CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC
    AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC
    TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT
    GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT
    TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT
    GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG
    GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA
    GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA
    CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA
    GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT
    ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC
    ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT
    AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC
    AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG
    ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA
    ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT
    GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC
    ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC
    ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG
    ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT
    CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC
    ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT
    CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA
    GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC
    TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA
    CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA
    GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC
    ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT
    AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA
    TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA
    TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG
    ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.