PRG4 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017000002.1	XP_016855491.1	proteoglycan 4 isoform X1
XM_017000003.1	XP_016855492.1	proteoglycan 4 isoform X2
NM_001303232.1	NP_001290161.1	proteoglycan 4 isoform E preproprotein
NM_001127709.2	NP_001121181.2	proteoglycan 4 isoform C preproprotein
NM_005807.5	NP_005798.3	proteoglycan 4 isoform A preproprotein
NM_001303232.2	NP_001290161.1	proteoglycan 4 isoform E preproprotein
NM_001127710.2	NP_001121182.2	proteoglycan 4 isoform D preproprotein
NM_001127710.3	NP_001121182.2	proteoglycan 4 isoform D preproprotein
NM_001127709.3	NP_001121181.2	proteoglycan 4 isoform C preproprotein
XM_024448717.1	XP_024304485.1	proteoglycan 4 isoform X2
NM_005807.4	NP_005798.3	proteoglycan 4 isoform A preproprotein
NM_001127708.2	NP_001121180.2	proteoglycan 4 isoform B preproprotein
NM_005807.6	NP_005798.3	proteoglycan 4 isoform A preproprotein
XM_024448707.1	XP_024304475.1	proteoglycan 4 isoform X1
NM_001127708.3	NP_001121180.2	proteoglycan 4 isoform B preproprotein

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu63780	NM_005807.6 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant A, also known as V0, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu34060	NM_001303232.2 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu63778	NM_001127709.3 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu63779	NM_001127708.3 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu63781	NM_001127710.3 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu63778	XM_017000002.1 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu63779	XM_024448717.1 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu63780	XM_024448707.1 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu63781	XM_017000003.1 Latest version!	Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu34060	NM_001303232.1	Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu63781	NM_001127710.2	Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu63778	NM_001127709.2	Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu63780	NM_005807.4	Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu63780	NM_005807.5	Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu63779	NM_001127708.2	Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

PRG4 ( NM_005807.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005798.3 Homo sapiens proteoglycan 4 (PRG4), transcript variant A, also known as V0, mRNA.

Price & Availability↑

CloneID	OHu63780
Clone ID Related Accession (Same CDS sequence)	NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
Accession Version	NM_005807.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-29
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133553.9 and KF455089.1. This sequence is a reference standard in the RefSeqGene project. On Oct 29, 2019 this sequence version replaced NM_005807.5. Transcript Variant: This variant (A, also known as V0) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70136.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005798.3
CDS	46..4260
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_005807.6
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant A, also known as V0, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005807.6

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001303232.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290161.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.

Price & Availability↑

CloneID	OHu34060
Clone ID Related Accession (Same CDS sequence)	NM_001303232.1 , NM_001303232.2
Accession Version	NM_001303232.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA995681.1, U70136.1, AL133553.9 and CA435357.1. On Dec 23, 2014 this sequence version replaced XM_005244822.2. Transcript Variant: This variant (E, also known as V1) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform E) is shorter than isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11518279, 14976050 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC 
AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA 
AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC 
AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT 
CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA 
GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG 
ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC 
ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT 
CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC 
AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC 
ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT 
GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT 
CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA 
CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG 
TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC 
ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG 
CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC 
ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG 
CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG 
ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT 
ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC 
ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT 
AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT 
CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC 
AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA 
CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT 
CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC 
TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT 
ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC 
ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC 
AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA 
ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG 
CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG 
AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC 
CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA 
AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA 
ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT 
TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC 
AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT 
GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC 
AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC 
TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA 
AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA 
GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG 
AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA 
AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC 
TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC 
AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG 
AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC 
GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA 
ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290161.1
CDS	52..4137
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001303232.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303232.1

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC 
AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA 
AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC 
AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT 
CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA 
GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG 
ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC 
ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT 
CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC 
AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC 
ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT 
GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT 
CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA 
CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG 
TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC 
ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG 
CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC 
ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG 
CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG 
ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT 
ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC 
ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT 
AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT 
CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC 
AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA 
CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT 
CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC 
TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT 
ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC 
ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC 
AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA 
ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG 
CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG 
AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC 
CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA 
AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA 
ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT 
TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC 
AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT 
GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC 
AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC 
TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA 
AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA 
GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG 
AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA 
AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC 
TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC 
AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG 
AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC 
GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA 
ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001303232.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290161.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.

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CloneID	OHu34060
Clone ID Related Accession (Same CDS sequence)	NM_001303232.1 , NM_001303232.2
Accession Version	NM_001303232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA995681.1, U70136.1, AL133553.9 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001303232.1. Transcript Variant: This variant (E, also known as V1) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform E) is shorter than isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11518279, 14976050 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC 
AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA 
AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC 
AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT 
CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA 
GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG 
ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC 
ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT 
CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC 
AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC 
ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT 
GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT 
CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA 
CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG 
TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC 
ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG 
CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC 
ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG 
CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG 
ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT 
ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC 
ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT 
AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT 
CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC 
AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA 
CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT 
CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC 
TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT 
ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC 
ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC 
AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA 
ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG 
CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG 
AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC 
CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA 
AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA 
ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT 
TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC 
AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT 
GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC 
AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC 
TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA 
AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA 
GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG 
AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA 
AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC 
TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC 
AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG 
AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC 
GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA 
ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290161.1
CDS	52..4137
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001303232.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303232.2

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGT AAAAGATAAC 
AAGAAGAACA GAACTAAAAA GAAACCTACC CCCAAACCAC CAGTTGTAGA TGAAGCTGGA 
AGTGGATTGG ACAATGGTGA CTTCAAGGTC ACAACTCCTG ACACGTCTAC CACCCAACAC 
AATAAAGTCA GCACATCTCC CAAGATCACA ACAGCAAAAC CAATAAATCC CAGACCCAGT 
CTTCCACCTA ATTCTGATAC ATCTAAAGAG ACGTCTTTGA CAGTGAATAA AGAGACAACA 
GTTGAAACTA AAGAAACTAC TACAACAAAT AAACAGACTT CAACTGATGG AAAAGAGAAG 
ACTACTTCCG CTAAAGAGAC ACAAAGTATA GAGAAAACAT CTGCTAAAGA TTTAGCACCC 
ACATCTAAAG TGCTGGCTAA ACCTACACCC AAAGCTGAAA CTACAACCAA AGGCCCTGCT 
CTCACCACTC CCAAGGAGCC CACGCCCACC ACTCCCAAGG AGCCTGCATC TACCACACCC 
AAAGAGCCCA CACCTACCAC CATCAAGTCT GCACCCACCA CCCCCAAGGA GCCTGCACCC 
ACCACCACCA AGTCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACCACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCCC CAAGAAGCCT 
GCCCCAACTA CCCCCAAGGA GCCTGCACCC ACCACTCCCA AGGAGCCTAC ACCCACCACT 
CCCAAGGAGC CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA AGAGCCTGCA 
CCCACTGCCC CCAAGAAGCC TGCCCCAACT ACCCCCAAGG AGCCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTTCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT ACCAAGGAGC CTGCACCCAC CACTACCAAG 
TCTGCACCCA CCACTCCCAA GGAGCCTTCA CCCACCACCA CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG 
CCTGCACCCA CCACTCCCAA GGAACCTGCA CCCACCACCA CCAAGAAGCC TGCACCCACC 
ACTCCCAAAG AGCCTGCCCC AACTACCCCC AAGGAGACTG CACCCACCAC CCCCAAGAAG 
CTCACGCCCA CCACCCCCGA GAAGCTCGCA CCCACCACCC CTGAGAAGCC CGCACCCACC 
ACCCCTGAGG AGCTCGCACC CACCACCCCT GAGGAGCCCA CACCCACCAC CCCTGAGGAG 
CCTGCTCCCA CCACTCCCAA GGCAGCGGCT CCCAACACCC CTAAGGAGCC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG 
ACTGCTCCAA CTACCCCTAA AGGGACTGCT CCAACTACCC TCAAGGAACC TGCACCCACT 
ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT GCACCCACCA CCACCAAGGA GCCCACATCC 
ACCACCTCTG ACAAGCCCGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGCCT GCTCCAACTA CCCCTAAGGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGGGCC CACATCCACC ACCTCTGACA AGCCTGCTCC AACTACACCT 
AAGGAGACTG CTCCAACTAC CCCCAAGGAG CCTGCACCCA CTACCCCCAA GAAGCCTGCT 
CCAACTACTC CTGAGACACC TCCTCCAACC ACTTCAGAGG TCTCTACTCC AACTACCACC 
AAGGAGCCTA CCACTATCCA CAAAAGCCCT GATGAATCAA CTCCTGAGCT TTCTGCAGAA 
CCCACACCAA AAGCTCTTGA AAACAGTCCC AAGGAACCTG GTGTACCTAC AACTAAGACT 
CCTGCAGCGA CTAAACCTGA AATGACTACA ACAGCTAAAG ACAAGACAAC AGAAAGAGAC 
TTACGTACTA CACCTGAAAC TACAACTGCT GCACCTAAGA TGACAAAAGA GACAGCAACT 
ACAACAGAAA AAACTACCGA ATCCAAAATA ACAGCTACAA CCACACAAGT AACATCTACC 
ACAACTCAAG ATACCACACC ATTCAAAATT ACTACTCTTA AAACAACTAC TCTTGCACCC 
AAAGTAACTA CAACAAAAAA GACAATTACT ACCACTGAGA TTATGAACAA ACCTGAAGAA 
ACAGCTAAAC CAAAAGACAG AGCTACTAAT TCTAAAGCGA CAACTCCTAA ACCTCAAAAG 
CCAACCAAAG CACCCAAAAA ACCCACTTCT ACCAAAAAGC CAAAAACAAT GCCTAGAGTG 
AGAAAACCAA AGACGACACC AACTCCCCGC AAGATGACAT CAACAATGCC AGAATTGAAC 
CCTACCTCAA GAATAGCAGA AGCCATGCTC CAAACCACCA CCAGACCTAA CCAAACTCCA 
AACTCCAAAC TAGTTGAAGT AAATCCAAAG AGTGAAGATG CAGGTGGTGC TGAAGGAGAA 
ACACCTCATA TGCTTCTCAG GCCCCATGTG TTCATGCCTG AAGTTACTCC CGACATGGAT 
TACTTACCGA GAGTACCCAA TCAAGGCATT ATCATCAATC CCATGCTTTC CGATGAGACC 
AATATATGCA ATGGTAAGCC AGTAGATGGA CTGACTACTT TGCGCAATGG GACATTAGTT 
GCATTCCGAG GTCATTATTT CTGGATGCTA AGTCCATTCA GTCCACCATC TCCAGCTCGC 
AGAATTACTG AAGTTTGGGG TATTCCTTCC CCCATTGATA CTGTTTTTAC TAGGTGCAAC 
TGTGAAGGAA AAACTTTCTT CTTTAAGGAT TCTCAGTACT GGCGTTTTAC CAATGATATA 
AAAGATGCAG GGTACCCCAA ACCAATTTTC AAAGGATTTG GAGGACTAAC TGGACAAATA 
GTGGCAGCGC TTTCAACAGC TAAATATAAG AACTGGCCTG AATCTGTGTA TTTTTTCAAG 
AGAGGTGGCA GCATTCAGCA GTATATTTAT AAACAGGAAC CTGTACAGAA GTGCCCTGGA 
AGAAGGCCTG CTCTAAATTA TCCAGTGTAT GGAGAAACGA CACAGGTTAG GAGACGTCGC 
TTTGAACGTG CTATAGGACC TTCTCAAACA CACACCATCA GAATTCAATA TTCACCTGCC 
AGACTGGCTT ATCAAGACAA AGGTGTCCTT CATAATGAAG TTAAAGTGAG TATACTGTGG 
AGAGGACTTC CAAATGTGGT TACCTCAGCT ATATCACTGC CCAACATCAG AAAACCTGAC 
GGCTATGATT ACTATGCCTT TTCTAAAGAT CAATACTATA ACATTGATGT GCCTAGTAGA 
ACAGCAAGAG CAATTACTAC TCGTTCTGGG CAGACCTTAT CCAAAGTCTG GTACAACTGT 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001127709.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121181.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.

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CloneID	OHu63778
Clone ID Related Accession (Same CDS sequence)	XM_017000002.1 , NM_001127709.2 , NM_001127709.3
Accession Version	NM_001127709.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform C preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC404857.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Dec 23, 2014 this sequence version replaced NM_001127709.1. Transcript Variant: This variant (C, also known as V2) lacks two alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform C) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC 
CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC 
ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA 
ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG 
ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT 
AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA 
GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC 
AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC 
ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT 
GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT 
ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT 
ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA 
CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC 
AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA 
CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT 
GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT 
CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT 
CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT 
GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC 
CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT 
CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC 
ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG 
CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC 
ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT 
GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC 
AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA 
ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT 
GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA 
ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT 
ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT 
ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT 
TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT 
ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC 
AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC 
CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG 
AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG 
TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT 
ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA 
CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA 
AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC 
CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC 
AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG 
AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT 
AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT 
GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA 
CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT 
CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT 
ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT 
CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG 
CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121181.2
CDS	52..3987
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001127709.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127709.2

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC 
CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC 
ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA 
ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG 
ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT 
AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA 
GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC 
AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC 
ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT 
GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT 
ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT 
ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA 
CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC 
AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA 
CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT 
GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT 
CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT 
CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT 
GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC 
CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT 
CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC 
ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG 
CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC 
ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT 
GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC 
AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA 
ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT 
GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA 
ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT 
ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT 
ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT 
TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT 
ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC 
AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC 
CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG 
AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG 
TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT 
ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA 
CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA 
AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC 
CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC 
AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG 
AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT 
AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT 
GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA 
CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT 
CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT 
ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT 
CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG 
CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001127709.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121181.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.

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CloneID	OHu63778
Clone ID Related Accession (Same CDS sequence)	XM_017000002.1 , NM_001127709.2 , NM_001127709.3
Accession Version	NM_001127709.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-29
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform C preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC404857.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001127709.2. Transcript Variant: This variant (C, also known as V2) lacks two alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform C) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC 
CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC 
ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA 
ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG 
ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT 
AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA 
GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC 
AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC 
ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT 
GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT 
ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT 
ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA 
CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC 
AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA 
CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT 
GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT 
CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT 
CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT 
GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC 
CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT 
CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC 
ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG 
CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC 
ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT 
GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC 
AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA 
ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT 
GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA 
ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT 
ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT 
ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT 
TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT 
ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC 
AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC 
CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG 
AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG 
TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT 
ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA 
CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA 
AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC 
CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC 
AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG 
AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT 
AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT 
GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA 
CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT 
CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT 
ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT 
CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG 
CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121181.2
CDS	52..3987
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001127709.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127709.3

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC 
CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC 
ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA 
ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG 
ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT 
AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA 
GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC 
AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC 
ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT 
GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT 
ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT 
ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA 
CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC 
AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA 
CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT 
GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT 
CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT 
CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT 
GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC 
CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT 
CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC 
ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG 
CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC 
ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT 
GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC 
AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA 
ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT 
GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA 
ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT 
ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT 
ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT 
TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT 
ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC 
AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC 
CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG 
AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG 
TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT 
ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA 
CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA 
AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC 
CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC 
AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG 
AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT 
AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT 
GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA 
CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT 
CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT 
ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT 
CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG 
CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001127708.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121180.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.

Price & Availability↑

CloneID	OHu63779
Clone ID Related Accession (Same CDS sequence)	XM_024448717.1 , NM_001127708.2 , NM_001127708.3
Accession Version	NM_001127708.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, AK131434.1, AL133553.9, KF455089.1, U70136.1 and CA435357.1. On Dec 23, 2014 this sequence version replaced NM_001127708.1. Transcript Variant: This variant (B, also known as V0a) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform B) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA 
CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC 
AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT 
GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA 
ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA 
GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA 
GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC 
CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA 
CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG 
ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA 
GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA 
GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC 
CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC 
ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG 
CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT 
ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA 
CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC 
AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA 
CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT 
GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA 
CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC 
ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC 
CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT 
GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG 
CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT 
ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG 
CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT 
ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT 
GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT 
AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA 
AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA 
GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA 
TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT 
GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT 
GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT 
CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT 
AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA 
TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA 
ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA 
GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC 
ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT 
GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA 
TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA 
GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG 
TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT 
GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA 
CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT 
TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC 
CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA 
CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA 
CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA 
CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA 
CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT 
AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC 
AACTGTCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121180.2
CDS	52..4143
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001127708.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127708.2

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA 
CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC 
AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT 
GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA 
ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA 
GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA 
GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC 
CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA 
CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG 
ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA 
GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA 
GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC 
CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC 
ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG 
CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT 
ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA 
CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC 
AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA 
CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT 
GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA 
CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC 
ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC 
CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT 
GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG 
CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT 
ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG 
CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT 
ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT 
GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT 
AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA 
AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA 
GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA 
TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT 
GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT 
GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT 
CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT 
AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA 
TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA 
ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA 
GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC 
ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT 
GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA 
TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA 
GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG 
TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT 
GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA 
CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT 
TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC 
CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA 
CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA 
CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA 
CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA 
CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT 
AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC 
AACTGTCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001127708.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121180.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.

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CloneID	OHu63779
Clone ID Related Accession (Same CDS sequence)	XM_024448717.1 , NM_001127708.2 , NM_001127708.3
Accession Version	NM_001127708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, AK131434.1, AL133553.9, KF455089.1, U70136.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001127708.2. Transcript Variant: This variant (B, also known as V0a) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform B) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA 
CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC 
AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT 
GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA 
ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA 
GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA 
GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC 
CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA 
CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG 
ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA 
GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA 
GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC 
CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC 
ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG 
CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT 
ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA 
CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC 
AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA 
CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT 
GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA 
CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC 
ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC 
CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT 
GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG 
CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT 
ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG 
CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT 
ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT 
GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT 
AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA 
AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA 
GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA 
TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT 
GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT 
GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT 
CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT 
AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA 
TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA 
ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA 
GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC 
ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT 
GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA 
TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA 
GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG 
TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT 
GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA 
CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT 
TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC 
CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA 
CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA 
CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA 
CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA 
CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT 
AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC 
AACTGTCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121180.2
CDS	52..4143
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001127708.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127708.3

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA 
CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC 
AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT 
GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA 
ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA 
GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA 
GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC 
CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA 
CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG 
ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA 
GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA 
GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC 
CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC 
ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG 
CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT 
ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA 
CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC 
AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA 
CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT 
GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA 
CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC 
ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC 
CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT 
GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG 
CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT 
ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG 
CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT 
ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT 
GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT 
AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA 
AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA 
GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA 
TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT 
GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT 
GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT 
CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT 
AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA 
TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA 
ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA 
GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC 
ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT 
GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA 
TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA 
GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG 
TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT 
GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA 
CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT 
TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC 
CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA 
CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA 
CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA 
CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA 
CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT 
AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC 
AACTGTCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_005807.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005798.3 Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu63780
Clone ID Related Accession (Same CDS sequence)	NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
Accession Version	NM_005807.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, U70136.1, AK131434.1, AL133553.9, KF455089.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_005807.4. Transcript Variant: This variant (A, also known as V0) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70136.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005798.3
CDS	52..4266
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_005807.5
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005807.5

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_005807.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005798.3 Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu63780
Clone ID Related Accession (Same CDS sequence)	NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
Accession Version	NM_005807.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, U70136.1, AK131434.1, AL133553.9, KF455089.1 and CA435357.1. This sequence is a reference standard in the RefSeqGene project. On Dec 23, 2014 this sequence version replaced NM_005807.3. Transcript Variant: This variant (A, also known as V0) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U70136.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005798.3
CDS	52..4266
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_005807.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005807.4

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001127710.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121182.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.

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CloneID	OHu63781
Clone ID Related Accession (Same CDS sequence)	XM_017000003.1 , NM_001127710.2 , NM_001127710.3
Accession Version	NM_001127710.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform D preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC295855.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Dec 23, 2014 this sequence version replaced NM_001127710.1. Transcript Variant: This variant (D, also known as V3) lacks three alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform D) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC 
AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA 
ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA 
GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG 
TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA 
CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG 
AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA 
GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT 
CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA 
CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC 
AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC 
ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG 
GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC 
ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG 
GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC 
AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT 
AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC 
AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC 
TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT 
GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT 
TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT 
GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG 
GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA 
GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA 
CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA 
GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT 
ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC 
ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT 
AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC 
AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG 
ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA 
ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT 
GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC 
ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC 
ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG 
ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT 
CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC 
ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT 
CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA 
GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC 
TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA 
CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA 
GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC 
ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT 
AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA 
TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA 
TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG 
ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121182.2
CDS	52..3864
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001127710.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127710.2

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC 
AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA 
ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA 
GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG 
TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA 
CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG 
AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA 
GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT 
CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA 
CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC 
AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC 
ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG 
GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC 
ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG 
GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC 
AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT 
AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC 
AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC 
TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT 
GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT 
TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT 
GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG 
GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA 
GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA 
CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA 
GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT 
ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC 
ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT 
AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC 
AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG 
ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA 
ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT 
GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC 
ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC 
ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG 
ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT 
CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC 
ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT 
CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA 
GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC 
TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA 
CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA 
GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC 
ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT 
AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA 
TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA 
TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG 
ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( NM_001127710.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121182.2 Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.

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CloneID	OHu63781
Clone ID Related Accession (Same CDS sequence)	XM_017000003.1 , NM_001127710.2 , NM_001127710.3
Accession Version	NM_001127710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform D preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA977744.1, DC295855.1, AL133553.9, KF455089.1, AK131434.1, U70136.1 and CA435357.1. On Jun 2, 2019 this sequence version replaced NM_001127710.2. Transcript Variant: This variant (D, also known as V3) lacks three alternate in-frame exons in the 5' coding region, compared to variant A. The resulting protein (isoform D) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9920774, 11124536 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC 
AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA 
ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA 
GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG 
TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA 
CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG 
AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA 
GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT 
CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA 
CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC 
AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC 
ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG 
GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC 
ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG 
GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC 
AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT 
AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC 
AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC 
TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT 
GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT 
TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT 
GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG 
GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA 
GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA 
CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA 
GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT 
ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC 
ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT 
AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC 
AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG 
ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA 
ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT 
GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC 
ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC 
ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG 
ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT 
CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC 
ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT 
CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA 
GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC 
TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA 
CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA 
GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC 
ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT 
AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA 
TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA 
TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG 
ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121182.2
CDS	52..3864
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	NM_001127710.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127710.3

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC 
AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA 
ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA 
GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG 
TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA 
CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG 
AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA 
GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT 
CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA 
CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC 
AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC 
ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG 
GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC 
ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG 
GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC 
AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT 
AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC 
AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC 
TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT 
GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT 
TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT 
GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG 
GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA 
GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA 
CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA 
GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT 
ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC 
ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT 
AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC 
AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG 
ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA 
ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT 
GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC 
ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC 
ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG 
ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT 
CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC 
ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT 
CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA 
GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC 
TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA 
CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA 
GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC 
ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT 
AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA 
TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA 
TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG 
ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( XM_017000002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855491.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63778
Clone ID Related Accession (Same CDS sequence)	XM_017000002.1 , NM_001127709.2 , NM_001127709.3
Accession Version	XM_017000002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC 
CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC 
ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA 
ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG 
ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT 
AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA 
GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC 
AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC 
ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT 
GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT 
ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT 
ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA 
CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC 
AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA 
CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT 
GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT 
CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT 
CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT 
GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC 
CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT 
CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC 
ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG 
CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC 
ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT 
GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC 
AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA 
ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT 
GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA 
ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT 
ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT 
ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT 
TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT 
ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC 
AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC 
CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG 
AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG 
TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT 
ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA 
CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA 
AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC 
CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC 
AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG 
AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT 
AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT 
GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA 
CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT 
CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT 
ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT 
CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG 
CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855491.1
CDS	348..4283
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	XM_017000002.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000002.1

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT AAAAGATAAC AAGAAGAACA GAACTAAAAA GAAACCTACC 
CCCAAACCAC CAGTTGTAGA TGAAGCTGGA AGTGGATTGG ACAATGGTGA CTTCAAGGTC 
ACAACTCCTG ACACGTCTAC CACCCAACAC AATAAAGTCA GCACATCTCC CAAGATCACA 
ACAGCAAAAC CAATAAATCC CAGACCCAGT CTTCCACCTA ATTCTGATAC ATCTAAAGAG 
ACGTCTTTGA CAGTGAATAA AGAGACAACA GTTGAAACTA AAGAAACTAC TACAACAAAT 
AAACAGACTT CAACTGATGG AAAAGAGAAG ACTACTTCCG CTAAAGAGAC ACAAAGTATA 
GAGAAAACAT CTGCTAAAGA TTTAGCACCC ACATCTAAAG TGCTGGCTAA ACCTACACCC 
AAAGCTGAAA CTACAACCAA AGGCCCTGCT CTCACCACTC CCAAGGAGCC CACGCCCACC 
ACTCCCAAGG AGCCTGCATC TACCACACCC AAAGAGCCCA CACCTACCAC CATCAAGTCT 
GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTCCC 
AAGGAGCCTG CACCCACCAC CACCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTAC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCAAGGAG 
CCTGCACCCA CCACTCCCAA AGAGCCTGCA CCCACTGCCC CCAAGAAGCC TGCCCCAACT 
ACCCCCAAGG AGCCTGCACC CACCACTCCC AAGGAGCCTG CACCCACCAC CACCAAGGAG 
CCTTCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGTCTGC ACCCACCACT 
ACCAAGGAGC CTGCACCCAC CACTACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTTCA 
CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAACCTGCA 
CCCACCACCA CCAAGAAGCC TGCACCCACC ACTCCCAAAG AGCCTGCCCC AACTACCCCC 
AAGGAGACTG CACCCACCAC CCCCAAGAAG CTCACGCCCA CCACCCCCGA GAAGCTCGCA 
CCCACCACCC CTGAGAAGCC CGCACCCACC ACCCCTGAGG AGCTCGCACC CACCACCCCT 
GAGGAGCCCA CACCCACCAC CCCTGAGGAG CCTGCTCCCA CCACTCCCAA GGCAGCGGCT 
CCCAACACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGCCTG CTCCAACTAC CCCTAAGGAG ACTGCTCCAA CTACCCCTAA AGGGACTGCT 
CCAACTACCC TCAAGGAACC TGCACCCACT ACTCCCAAGA AGCCTGCCCC CAAGGAGCTT 
GCACCCACCA CCACCAAGGA GCCCACATCC ACCACCTCTG ACAAGCCCGC TCCAACTACC 
CCTAAGGGGA CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGG GACTGCTCCA ACTACCCTCA AGGAACCTGC ACCCACTACT 
CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA CCCACCACCA CCAAGGGGCC CACATCCACC 
ACCTCTGACA AGCCTGCTCC AACTACACCT AAGGAGACTG CTCCAACTAC CCCCAAGGAG 
CCTGCACCCA CTACCCCCAA GAAGCCTGCT CCAACTACTC CTGAGACACC TCCTCCAACC 
ACTTCAGAGG TCTCTACTCC AACTACCACC AAGGAGCCTA CCACTATCCA CAAAAGCCCT 
GATGAATCAA CTCCTGAGCT TTCTGCAGAA CCCACACCAA AAGCTCTTGA AAACAGTCCC 
AAGGAACCTG GTGTACCTAC AACTAAGACT CCTGCAGCGA CTAAACCTGA AATGACTACA 
ACAGCTAAAG ACAAGACAAC AGAAAGAGAC TTACGTACTA CACCTGAAAC TACAACTGCT 
GCACCTAAGA TGACAAAAGA GACAGCAACT ACAACAGAAA AAACTACCGA ATCCAAAATA 
ACAGCTACAA CCACACAAGT AACATCTACC ACAACTCAAG ATACCACACC ATTCAAAATT 
ACTACTCTTA AAACAACTAC TCTTGCACCC AAAGTAACTA CAACAAAAAA GACAATTACT 
ACCACTGAGA TTATGAACAA ACCTGAAGAA ACAGCTAAAC CAAAAGACAG AGCTACTAAT 
TCTAAAGCGA CAACTCCTAA ACCTCAAAAG CCAACCAAAG CACCCAAAAA ACCCACTTCT 
ACCAAAAAGC CAAAAACAAT GCCTAGAGTG AGAAAACCAA AGACGACACC AACTCCCCGC 
AAGATGACAT CAACAATGCC AGAATTGAAC CCTACCTCAA GAATAGCAGA AGCCATGCTC 
CAAACCACCA CCAGACCTAA CCAAACTCCA AACTCCAAAC TAGTTGAAGT AAATCCAAAG 
AGTGAAGATG CAGGTGGTGC TGAAGGAGAA ACACCTCATA TGCTTCTCAG GCCCCATGTG 
TTCATGCCTG AAGTTACTCC CGACATGGAT TACTTACCGA GAGTACCCAA TCAAGGCATT 
ATCATCAATC CCATGCTTTC CGATGAGACC AATATATGCA ATGGTAAGCC AGTAGATGGA 
CTGACTACTT TGCGCAATGG GACATTAGTT GCATTCCGAG GTCATTATTT CTGGATGCTA 
AGTCCATTCA GTCCACCATC TCCAGCTCGC AGAATTACTG AAGTTTGGGG TATTCCTTCC 
CCCATTGATA CTGTTTTTAC TAGGTGCAAC TGTGAAGGAA AAACTTTCTT CTTTAAGGAT 
TCTCAGTACT GGCGTTTTAC CAATGATATA AAAGATGCAG GGTACCCCAA ACCAATTTTC 
AAAGGATTTG GAGGACTAAC TGGACAAATA GTGGCAGCGC TTTCAACAGC TAAATATAAG 
AACTGGCCTG AATCTGTGTA TTTTTTCAAG AGAGGTGGCA GCATTCAGCA GTATATTTAT 
AAACAGGAAC CTGTACAGAA GTGCCCTGGA AGAAGGCCTG CTCTAAATTA TCCAGTGTAT 
GGAGAAACGA CACAGGTTAG GAGACGTCGC TTTGAACGTG CTATAGGACC TTCTCAAACA 
CACACCATCA GAATTCAATA TTCACCTGCC AGACTGGCTT ATCAAGACAA AGGTGTCCTT 
CATAATGAAG TTAAAGTGAG TATACTGTGG AGAGGACTTC CAAATGTGGT TACCTCAGCT 
ATATCACTGC CCAACATCAG AAAACCTGAC GGCTATGATT ACTATGCCTT TTCTAAAGAT 
CAATACTATA ACATTGATGT GCCTAGTAGA ACAGCAAGAG CAATTACTAC TCGTTCTGGG 
CAGACCTTAT CCAAAGTCTG GTACAACTGT CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( XM_024448717.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304485.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.

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CloneID	OHu63779
Clone ID Related Accession (Same CDS sequence)	XM_024448717.1 , NM_001127708.2 , NM_001127708.3
Accession Version	XM_024448717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA 
CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC 
AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT 
GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA 
ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA 
GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA 
GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC 
CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA 
CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG 
ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA 
GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA 
GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC 
CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC 
ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG 
CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT 
ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA 
CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC 
AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA 
CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT 
GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA 
CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC 
ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC 
CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT 
GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG 
CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT 
ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG 
CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT 
ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT 
GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT 
AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA 
AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA 
GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA 
TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT 
GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT 
GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT 
CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT 
AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA 
TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA 
ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA 
GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC 
ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT 
GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA 
TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA 
GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG 
TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT 
GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA 
CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT 
TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC 
CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA 
CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA 
CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA 
CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA 
CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT 
AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC 
AACTGTCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304485.1
CDS	38..4129
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	XM_024448717.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448717.1

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTGCA TAATCCCACA TCACCACCAT CTTCAAAGAA AGCACCTCCA 
CCTTCAGGAG CATCTCAAAC CATCAAATCA ACAACCAAAC GTTCACCCAA ACCACCAAAC 
AAGAAGAAGA CTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT 
GAAAATCAAG AGTCCTCCTC CTCCTCCTCC TCTTCCTCTT CTTCTTCAAC AATTCGGAAA 
ATCAAGTCTT CCAAAAATTC AGCTGCTAAT AGAGAATTAC AGAAGAAACT CAAAGTAAAA 
GATAACAAGA AGAACAGAAC TAAAAAGAAA CCTACCCCCA AACCACCAGT TGTAGATGAA 
GCTGGAAGTG GATTGGACAA TGGTGACTTC AAGGTCACAA CTCCTGACAC GTCTACCACC 
CAACACAATA AAGTCAGCAC ATCTCCCAAG ATCACAACAG CAAAACCAAT AAATCCCAGA 
CCCAGTCTTC CACCTAATTC TGATACATCT AAAGAGACGT CTTTGACAGT GAATAAAGAG 
ACAACAGTTG AAACTAAAGA AACTACTACA ACAAATAAAC AGACTTCAAC TGATGGAAAA 
GAGAAGACTA CTTCCGCTAA AGAGACACAA AGTATAGAGA AAACATCTGC TAAAGATTTA 
GCACCCACAT CTAAAGTGCT GGCTAAACCT ACACCCAAAG CTGAAACTAC AACCAAAGGC 
CCTGCTCTCA CCACTCCCAA GGAGCCCACG CCCACCACTC CCAAGGAGCC TGCATCTACC 
ACACCCAAAG AGCCCACACC TACCACCATC AAGTCTGCAC CCACCACCCC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACCA CCAAGTCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTACACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC AAGGAGCCTG CACCCACCAC TCCCAAAGAG 
CCTGCACCCA CTGCCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTT CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTACCA AGGAGCCTGC ACCCACCACT 
ACCAAGTCTG CACCCACCAC TCCCAAGGAG CCTTCACCCA CCACCACCAA GGAGCCTGCA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCCCCAAGA AGCCTGCCCC AACTACCCCC 
AAGGAGCCTG CACCCACCAC TCCCAAGGAA CCTGCACCCA CCACCACCAA GAAGCCTGCA 
CCCACCACTC CCAAAGAGCC TGCCCCAACT ACCCCCAAGG AGACTGCACC CACCACCCCC 
AAGAAGCTCA CGCCCACCAC CCCCGAGAAG CTCGCACCCA CCACCCCTGA GAAGCCCGCA 
CCCACCACCC CTGAGGAGCT CGCACCCACC ACCCCTGAGG AGCCCACACC CACCACCCCT 
GAGGAGCCTG CTCCCACCAC TCCCAAGGCA GCGGCTCCCA ACACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGAGCC TGCTCCAACT ACCCCTAAGG AGCCTGCTCC AACTACCCCT 
AAGGAGACTG CTCCAACTAC CCCTAAAGGG ACTGCTCCAA CTACCCTCAA GGAACCTGCA 
CCCACTACTC CCAAGAAGCC TGCCCCCAAG GAGCTTGCAC CCACCACCAC CAAGGAGCCC 
ACATCCACCA CCTCTGACAA GCCCGCTCCA ACTACCCCTA AGGGGACTGC TCCAACTACC 
CCTAAGGAGC CTGCTCCAAC TACCCCTAAG GAGCCTGCTC CAACTACCCC TAAGGGGACT 
GCTCCAACTA CCCTCAAGGA ACCTGCACCC ACTACTCCCA AGAAGCCTGC CCCCAAGGAG 
CTTGCACCCA CCACCACCAA GGGGCCCACA TCCACCACCT CTGACAAGCC TGCTCCAACT 
ACACCTAAGG AGACTGCTCC AACTACCCCC AAGGAGCCTG CACCCACTAC CCCCAAGAAG 
CCTGCTCCAA CTACTCCTGA GACACCTCCT CCAACCACTT CAGAGGTCTC TACTCCAACT 
ACCACCAAGG AGCCTACCAC TATCCACAAA AGCCCTGATG AATCAACTCC TGAGCTTTCT 
GCAGAACCCA CACCAAAAGC TCTTGAAAAC AGTCCCAAGG AACCTGGTGT ACCTACAACT 
AAGACTCCTG CAGCGACTAA ACCTGAAATG ACTACAACAG CTAAAGACAA GACAACAGAA 
AGAGACTTAC GTACTACACC TGAAACTACA ACTGCTGCAC CTAAGATGAC AAAAGAGACA 
GCAACTACAA CAGAAAAAAC TACCGAATCC AAAATAACAG CTACAACCAC ACAAGTAACA 
TCTACCACAA CTCAAGATAC CACACCATTC AAAATTACTA CTCTTAAAAC AACTACTCTT 
GCACCCAAAG TAACTACAAC AAAAAAGACA ATTACTACCA CTGAGATTAT GAACAAACCT 
GAAGAAACAG CTAAACCAAA AGACAGAGCT ACTAATTCTA AAGCGACAAC TCCTAAACCT 
CAAAAGCCAA CCAAAGCACC CAAAAAACCC ACTTCTACCA AAAAGCCAAA AACAATGCCT 
AGAGTGAGAA AACCAAAGAC GACACCAACT CCCCGCAAGA TGACATCAAC AATGCCAGAA 
TTGAACCCTA CCTCAAGAAT AGCAGAAGCC ATGCTCCAAA CCACCACCAG ACCTAACCAA 
ACTCCAAACT CCAAACTAGT TGAAGTAAAT CCAAAGAGTG AAGATGCAGG TGGTGCTGAA 
GGAGAAACAC CTCATATGCT TCTCAGGCCC CATGTGTTCA TGCCTGAAGT TACTCCCGAC 
ATGGATTACT TACCGAGAGT ACCCAATCAA GGCATTATCA TCAATCCCAT GCTTTCCGAT 
GAGACCAATA TATGCAATGG TAAGCCAGTA GATGGACTGA CTACTTTGCG CAATGGGACA 
TTAGTTGCAT TCCGAGGTCA TTATTTCTGG ATGCTAAGTC CATTCAGTCC ACCATCTCCA 
GCTCGCAGAA TTACTGAAGT TTGGGGTATT CCTTCCCCCA TTGATACTGT TTTTACTAGG 
TGCAACTGTG AAGGAAAAAC TTTCTTCTTT AAGGATTCTC AGTACTGGCG TTTTACCAAT 
GATATAAAAG ATGCAGGGTA CCCCAAACCA ATTTTCAAAG GATTTGGAGG ACTAACTGGA 
CAAATAGTGG CAGCGCTTTC AACAGCTAAA TATAAGAACT GGCCTGAATC TGTGTATTTT 
TTCAAGAGAG GTGGCAGCAT TCAGCAGTAT ATTTATAAAC AGGAACCTGT ACAGAAGTGC 
CCTGGAAGAA GGCCTGCTCT AAATTATCCA GTGTATGGAG AAACGACACA GGTTAGGAGA 
CGTCGCTTTG AACGTGCTAT AGGACCTTCT CAAACACACA CCATCAGAAT TCAATATTCA 
CCTGCCAGAC TGGCTTATCA AGACAAAGGT GTCCTTCATA ATGAAGTTAA AGTGAGTATA 
CTGTGGAGAG GACTTCCAAA TGTGGTTACC TCAGCTATAT CACTGCCCAA CATCAGAAAA 
CCTGACGGCT ATGATTACTA TGCCTTTTCT AAAGATCAAT ACTATAACAT TGATGTGCCT 
AGTAGAACAG CAAGAGCAAT TACTACTCGT TCTGGGCAGA CCTTATCCAA AGTCTGGTAC 
AACTGTCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( XM_024448707.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304475.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63780
Clone ID Related Accession (Same CDS sequence)	NM_005807.5 , NM_005807.4 , NM_005807.6 , XM_024448707.1
Accession Version	XM_024448707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304475.1
CDS	38..4252
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	XM_024448707.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448707.1

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGATTT ATCAAGCTGT GCAGGGAGAT GTGGGGAAGG GTATTCTAGA 
GATGCCACCT GCAACTGTGA TTATAACTGT CAACACTACA TGGAGTGCTG CCCTGATTTC 
AAGAGAGTCT GCACTGCGGA GCTTTCCTGT AAAGGCCGCT GCTTTGAGTC CTTCGAGAGA 
GGGAGGGAGT GTGACTGCGA CGCCCAATGT AAGAAGTATG ACAAGTGCTG TCCCGATTAT 
GAGAGTTTCT GTGCAGAAGT GCATAATCCC ACATCACCAC CATCTTCAAA GAAAGCACCT 
CCACCTTCAG GAGCATCTCA AACCATCAAA TCAACAACCA AACGTTCACC CAAACCACCA 
AACAAGAAGA AGACTAAGAA AGTTATAGAA TCAGAGGAAA TAACAGAAGA ACATTCTGTT 
TCTGAAAATC AAGAGTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC AACAATTCGG 
AAAATCAAGT CTTCCAAAAA TTCAGCTGCT AATAGAGAAT TACAGAAGAA ACTCAAAGTA 
AAAGATAACA AGAAGAACAG AACTAAAAAG AAACCTACCC CCAAACCACC AGTTGTAGAT 
GAAGCTGGAA GTGGATTGGA CAATGGTGAC TTCAAGGTCA CAACTCCTGA CACGTCTACC 
ACCCAACACA ATAAAGTCAG CACATCTCCC AAGATCACAA CAGCAAAACC AATAAATCCC 
AGACCCAGTC TTCCACCTAA TTCTGATACA TCTAAAGAGA CGTCTTTGAC AGTGAATAAA 
GAGACAACAG TTGAAACTAA AGAAACTACT ACAACAAATA AACAGACTTC AACTGATGGA 
AAAGAGAAGA CTACTTCCGC TAAAGAGACA CAAAGTATAG AGAAAACATC TGCTAAAGAT 
TTAGCACCCA CATCTAAAGT GCTGGCTAAA CCTACACCCA AAGCTGAAAC TACAACCAAA 
GGCCCTGCTC TCACCACTCC CAAGGAGCCC ACGCCCACCA CTCCCAAGGA GCCTGCATCT 
ACCACACCCA AAGAGCCCAC ACCTACCACC ATCAAGTCTG CACCCACCAC CCCCAAGGAG 
CCTGCACCCA CCACCACCAA GTCTGCACCC ACCACTCCCA AGGAGCCTGC ACCCACCACC 
ACCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CCACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCCCC 
AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTACA 
CCCACCACTC CCAAGGAGCC TGCACCCACC ACCAAGGAGC CTGCACCCAC CACTCCCAAA 
GAGCCTGCAC CCACTGCCCC CAAGAAGCCT GCCCCAACTA CCCCCAAGGA GCCTGCACCC 
ACCACTCCCA AGGAGCCTGC ACCCACCACC ACCAAGGAGC CTTCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGTCTGCA CCCACCACTA CCAAGGAGCC TGCACCCACC 
ACTACCAAGT CTGCACCCAC CACTCCCAAG GAGCCTTCAC CCACCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAACCTGCAC CCACCACCAC CAAGAAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCCCCA ACTACCCCCA AGGAGACTGC ACCCACCACC 
CCCAAGAAGC TCACGCCCAC CACCCCCGAG AAGCTCGCAC CCACCACCCC TGAGAAGCCC 
GCACCCACCA CCCCTGAGGA GCTCGCACCC ACCACCCCTG AGGAGCCCAC ACCCACCACC 
CCTGAGGAGC CTGCTCCCAC CACTCCCAAG GCAGCGGCTC CCAACACCCC TAAGGAGCCT 
GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC 
CCTAAGGAGA CTGCTCCAAC TACCCCTAAA GGGACTGCTC CAACTACCCT CAAGGAACCT 
GCACCCACTA CTCCCAAGAA GCCTGCCCCC AAGGAGCTTG CACCCACCAC CACCAAGGAG 
CCCACATCCA CCACCTCTGA CAAGCCCGCT CCAACTACCC CTAAGGGGAC TGCTCCAACT 
ACCCCTAAGG AGCCTGCTCC AACTACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGGG 
ACTGCTCCAA CTACCCTCAA GGAACCTGCA CCCACTACTC CCAAGAAGCC TGCCCCCAAG 
GAGCTTGCAC CCACCACCAC CAAGGGGCCC ACATCCACCA CCTCTGACAA GCCTGCTCCA 
ACTACACCTA AGGAGACTGC TCCAACTACC CCCAAGGAGC CTGCACCCAC TACCCCCAAG 
AAGCCTGCTC CAACTACTCC TGAGACACCT CCTCCAACCA CTTCAGAGGT CTCTACTCCA 
ACTACCACCA AGGAGCCTAC CACTATCCAC AAAAGCCCTG ATGAATCAAC TCCTGAGCTT 
TCTGCAGAAC CCACACCAAA AGCTCTTGAA AACAGTCCCA AGGAACCTGG TGTACCTACA 
ACTAAGACTC CTGCAGCGAC TAAACCTGAA ATGACTACAA CAGCTAAAGA CAAGACAACA 
GAAAGAGACT TACGTACTAC ACCTGAAACT ACAACTGCTG CACCTAAGAT GACAAAAGAG 
ACAGCAACTA CAACAGAAAA AACTACCGAA TCCAAAATAA CAGCTACAAC CACACAAGTA 
ACATCTACCA CAACTCAAGA TACCACACCA TTCAAAATTA CTACTCTTAA AACAACTACT 
CTTGCACCCA AAGTAACTAC AACAAAAAAG ACAATTACTA CCACTGAGAT TATGAACAAA 
CCTGAAGAAA CAGCTAAACC AAAAGACAGA GCTACTAATT CTAAAGCGAC AACTCCTAAA 
CCTCAAAAGC CAACCAAAGC ACCCAAAAAA CCCACTTCTA CCAAAAAGCC AAAAACAATG 
CCTAGAGTGA GAAAACCAAA GACGACACCA ACTCCCCGCA AGATGACATC AACAATGCCA 
GAATTGAACC CTACCTCAAG AATAGCAGAA GCCATGCTCC AAACCACCAC CAGACCTAAC 
CAAACTCCAA ACTCCAAACT AGTTGAAGTA AATCCAAAGA GTGAAGATGC AGGTGGTGCT 
GAAGGAGAAA CACCTCATAT GCTTCTCAGG CCCCATGTGT TCATGCCTGA AGTTACTCCC 
GACATGGATT ACTTACCGAG AGTACCCAAT CAAGGCATTA TCATCAATCC CATGCTTTCC 
GATGAGACCA ATATATGCAA TGGTAAGCCA GTAGATGGAC TGACTACTTT GCGCAATGGG 
ACATTAGTTG CATTCCGAGG TCATTATTTC TGGATGCTAA GTCCATTCAG TCCACCATCT 
CCAGCTCGCA GAATTACTGA AGTTTGGGGT ATTCCTTCCC CCATTGATAC TGTTTTTACT 
AGGTGCAACT GTGAAGGAAA AACTTTCTTC TTTAAGGATT CTCAGTACTG GCGTTTTACC 
AATGATATAA AAGATGCAGG GTACCCCAAA CCAATTTTCA AAGGATTTGG AGGACTAACT 
GGACAAATAG TGGCAGCGCT TTCAACAGCT AAATATAAGA ACTGGCCTGA ATCTGTGTAT 
TTTTTCAAGA GAGGTGGCAG CATTCAGCAG TATATTTATA AACAGGAACC TGTACAGAAG 
TGCCCTGGAA GAAGGCCTGC TCTAAATTAT CCAGTGTATG GAGAAACGAC ACAGGTTAGG 
AGACGTCGCT TTGAACGTGC TATAGGACCT TCTCAAACAC ACACCATCAG AATTCAATAT 
TCACCTGCCA GACTGGCTTA TCAAGACAAA GGTGTCCTTC ATAATGAAGT TAAAGTGAGT 
ATACTGTGGA GAGGACTTCC AAATGTGGTT ACCTCAGCTA TATCACTGCC CAACATCAGA 
AAACCTGACG GCTATGATTA CTATGCCTTT TCTAAAGATC AATACTATAA CATTGATGTG 
CCTAGTAGAA CAGCAAGAGC AATTACTACT CGTTCTGGGC AGACCTTATC CAAAGTCTGG 
TACAACTGTC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRG4 ( XM_017000003.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855492.1 Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu63781
Clone ID Related Accession (Same CDS sequence)	XM_017000003.1 , NM_001127710.2 , NM_001127710.3
Accession Version	XM_017000003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	proteoglycan 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC 
AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA 
ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA 
GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG 
TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA 
CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG 
AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA 
GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT 
CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA 
CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC 
AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC 
ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG 
GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC 
ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG 
GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC 
AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT 
AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC 
AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC 
TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT 
GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT 
TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT 
GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG 
GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA 
GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA 
CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA 
GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT 
ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC 
ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT 
AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC 
AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG 
ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA 
ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT 
GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC 
ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC 
ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG 
ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT 
CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC 
ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT 
CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA 
GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC 
TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA 
CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA 
GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC 
ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT 
AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA 
TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA 
TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG 
ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855492.1
CDS	38..3850
Translation	MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Target ORF information:

RefSeq Version	XM_017000003.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteoglycan 4 (PRG4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000003.1

ORF Insert Sequence:

ATGGCATGGA AAACACTTCC CATTTACCTG TTGTTGCTGC TGTCTGTTTT CGTGATTCAG 
CAAGTTTCAT CTCAAGAGCT TTCCTGTAAA GGCCGCTGCT TTGAGTCCTT CGAGAGAGGG 
AGGGAGTGTG ACTGCGACGC CCAATGTAAG AAGTATGACA AGTGCTGTCC CGATTATGAG 
AGTTTCTGTG CAGAAGTAAA AGATAACAAG AAGAACAGAA CTAAAAAGAA ACCTACCCCC 
AAACCACCAG TTGTAGATGA AGCTGGAAGT GGATTGGACA ATGGTGACTT CAAGGTCACA 
ACTCCTGACA CGTCTACCAC CCAACACAAT AAAGTCAGCA CATCTCCCAA GATCACAACA 
GCAAAACCAA TAAATCCCAG ACCCAGTCTT CCACCTAATT CTGATACATC TAAAGAGACG 
TCTTTGACAG TGAATAAAGA GACAACAGTT GAAACTAAAG AAACTACTAC AACAAATAAA 
CAGACTTCAA CTGATGGAAA AGAGAAGACT ACTTCCGCTA AAGAGACACA AAGTATAGAG 
AAAACATCTG CTAAAGATTT AGCACCCACA TCTAAAGTGC TGGCTAAACC TACACCCAAA 
GCTGAAACTA CAACCAAAGG CCCTGCTCTC ACCACTCCCA AGGAGCCCAC GCCCACCACT 
CCCAAGGAGC CTGCATCTAC CACACCCAAA GAGCCCACAC CTACCACCAT CAAGTCTGCA 
CCCACCACCC CCAAGGAGCC TGCACCCACC ACCACCAAGT CTGCACCCAC CACTCCCAAG 
GAGCCTGCAC CCACCACCAC CAAGGAGCCT GCACCCACCA CTCCCAAGGA GCCTGCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACC ACCAAGTCTG CACCCACCAC TCCCAAGGAG 
CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC 
ACTCCCAAGG AGCCTACACC CACCACTCCC AAGGAGCCTG CACCCACCAC CAAGGAGCCT 
GCACCCACCA CTCCCAAAGA GCCTGCACCC ACTGCCCCCA AGAAGCCTGC CCCAACTACC 
CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTGCAC CCACCACCAC CAAGGAGCCT 
TCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCACCA AGTCTGCACC CACCACTACC 
AAGGAGCCTG CACCCACCAC TACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTTCACCC 
ACCACCACCA AGGAGCCTGC ACCCACCACT CCCAAGGAGC CTGCACCCAC CACCCCCAAG 
AAGCCTGCCC CAACTACCCC CAAGGAGCCT GCACCCACCA CTCCCAAGGA ACCTGCACCC 
ACCACCACCA AGAAGCCTGC ACCCACCACT CCCAAAGAGC CTGCCCCAAC TACCCCCAAG 
GAGACTGCAC CCACCACCCC CAAGAAGCTC ACGCCCACCA CCCCCGAGAA GCTCGCACCC 
ACCACCCCTG AGAAGCCCGC ACCCACCACC CCTGAGGAGC TCGCACCCAC CACCCCTGAG 
GAGCCCACAC CCACCACCCC TGAGGAGCCT GCTCCCACCA CTCCCAAGGC AGCGGCTCCC 
AACACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGAGC CTGCTCCAAC TACCCCTAAG 
GAGCCTGCTC CAACTACCCC TAAGGAGACT GCTCCAACTA CCCCTAAAGG GACTGCTCCA 
ACTACCCTCA AGGAACCTGC ACCCACTACT CCCAAGAAGC CTGCCCCCAA GGAGCTTGCA 
CCCACCACCA CCAAGGAGCC CACATCCACC ACCTCTGACA AGCCCGCTCC AACTACCCCT 
AAGGGGACTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT 
CCAACTACCC CTAAGGGGAC TGCTCCAACT ACCCTCAAGG AACCTGCACC CACTACTCCC 
AAGAAGCCTG CCCCCAAGGA GCTTGCACCC ACCACCACCA AGGGGCCCAC ATCCACCACC 
TCTGACAAGC CTGCTCCAAC TACACCTAAG GAGACTGCTC CAACTACCCC CAAGGAGCCT 
GCACCCACTA CCCCCAAGAA GCCTGCTCCA ACTACTCCTG AGACACCTCC TCCAACCACT 
TCAGAGGTCT CTACTCCAAC TACCACCAAG GAGCCTACCA CTATCCACAA AAGCCCTGAT 
GAATCAACTC CTGAGCTTTC TGCAGAACCC ACACCAAAAG CTCTTGAAAA CAGTCCCAAG 
GAACCTGGTG TACCTACAAC TAAGACTCCT GCAGCGACTA AACCTGAAAT GACTACAACA 
GCTAAAGACA AGACAACAGA AAGAGACTTA CGTACTACAC CTGAAACTAC AACTGCTGCA 
CCTAAGATGA CAAAAGAGAC AGCAACTACA ACAGAAAAAA CTACCGAATC CAAAATAACA 
GCTACAACCA CACAAGTAAC ATCTACCACA ACTCAAGATA CCACACCATT CAAAATTACT 
ACTCTTAAAA CAACTACTCT TGCACCCAAA GTAACTACAA CAAAAAAGAC AATTACTACC 
ACTGAGATTA TGAACAAACC TGAAGAAACA GCTAAACCAA AAGACAGAGC TACTAATTCT 
AAAGCGACAA CTCCTAAACC TCAAAAGCCA ACCAAAGCAC CCAAAAAACC CACTTCTACC 
AAAAAGCCAA AAACAATGCC TAGAGTGAGA AAACCAAAGA CGACACCAAC TCCCCGCAAG 
ATGACATCAA CAATGCCAGA ATTGAACCCT ACCTCAAGAA TAGCAGAAGC CATGCTCCAA 
ACCACCACCA GACCTAACCA AACTCCAAAC TCCAAACTAG TTGAAGTAAA TCCAAAGAGT 
GAAGATGCAG GTGGTGCTGA AGGAGAAACA CCTCATATGC TTCTCAGGCC CCATGTGTTC 
ATGCCTGAAG TTACTCCCGA CATGGATTAC TTACCGAGAG TACCCAATCA AGGCATTATC 
ATCAATCCCA TGCTTTCCGA TGAGACCAAT ATATGCAATG GTAAGCCAGT AGATGGACTG 
ACTACTTTGC GCAATGGGAC ATTAGTTGCA TTCCGAGGTC ATTATTTCTG GATGCTAAGT 
CCATTCAGTC CACCATCTCC AGCTCGCAGA ATTACTGAAG TTTGGGGTAT TCCTTCCCCC 
ATTGATACTG TTTTTACTAG GTGCAACTGT GAAGGAAAAA CTTTCTTCTT TAAGGATTCT 
CAGTACTGGC GTTTTACCAA TGATATAAAA GATGCAGGGT ACCCCAAACC AATTTTCAAA 
GGATTTGGAG GACTAACTGG ACAAATAGTG GCAGCGCTTT CAACAGCTAA ATATAAGAAC 
TGGCCTGAAT CTGTGTATTT TTTCAAGAGA GGTGGCAGCA TTCAGCAGTA TATTTATAAA 
CAGGAACCTG TACAGAAGTG CCCTGGAAGA AGGCCTGCTC TAAATTATCC AGTGTATGGA 
GAAACGACAC AGGTTAGGAG ACGTCGCTTT GAACGTGCTA TAGGACCTTC TCAAACACAC 
ACCATCAGAA TTCAATATTC ACCTGCCAGA CTGGCTTATC AAGACAAAGG TGTCCTTCAT 
AATGAAGTTA AAGTGAGTAT ACTGTGGAGA GGACTTCCAA ATGTGGTTAC CTCAGCTATA 
TCACTGCCCA ACATCAGAAA ACCTGACGGC TATGATTACT ATGCCTTTTC TAAAGATCAA 
TACTATAACA TTGATGTGCC TAGTAGAACA GCAAGAGCAA TTACTACTCG TTCTGGGCAG 
ACCTTATCCA AAGTCTGGTA CAACTGTCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PRG4
Entrez Gene ID	10216
Full Name	proteoglycan 4
Synonyms	CACP,HAPO,JCAP,MSF,SZP
General protein information	Preferred Names proteoglycan 4 Names proteoglycan 4 articular superficial zone protein hemangiopoietin lubricin megakaryocyte stimulating factor superficial zone proteoglycan
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q31.1
Summary	The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014].
Disorder MIM:	604283
Disorder Html:	Camptodactyly-arthropathy-coxa vara-pericarditis syndrome, 208250 (3)

Pan troglodytes (chimpanzee)	PRG4	XP_003308704.1
Rattus norvegicus (Norway rat)	Prg4	NP_001099432.2
Bos taurus (cattle)	PRG4	NP_001193562.1
Homo sapiens (human)	PRG4	NP_005798.2
	LOC716849	XP_001107843.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PRG4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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