PSMD14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMD14
Entrez Gene ID	10213
Full Name	proteasome 26S subunit, non-ATPase 14
Synonyms	PAD1,POH1,RPN11
General protein information	Preferred Names proteasome 26S subunit, non-ATPase 14 Names 26S proteasome non-ATPase regulatory subunit 14 26S proteasome regulatory subunit rpn11 26S proteasome-associated PAD1 homolog 1 proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 testis tissue sperm-binding protein Li 69n
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q24.2
Summary	This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012].
Disorder MIM:	607173

mRNA and Protein(s)

mRNA	Protein	Name
NM_005805.5	NP_005796.1	26S proteasome non-ATPase regulatory subunit 14
NM_005805.6	NP_005796.1	26S proteasome non-ATPase regulatory subunit 14

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PSMD14	XP_857441.2
Saccharomyces cerevisiae (baker's yeast)	RPN11	NP_116659.1
Arabidopsis thaliana (thale cress)	AT5G23540	NP_197745.1
Xenopus tropicalis (tropical clawed frog)	psmd14	NP_001025636.1
Pan troglodytes (chimpanzee)	PSMD14	XP_515855.2
Bos taurus (cattle)	PSMD14	NP_001069535.1
Gallus gallus (chicken)	PSMD14	NP_001026427.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008617	XP_314713.2
Caenorhabditis elegans (roundworm)	rpn-11	NP_494712.1
Eremothecium gossypii	AGOS_AGR006W	NP_986671.1
Magnaporthe oryzae (rice blast fungus)	MGG_16706	XP_003711682.1
Neurospora crassa	NCU00823	XP_964366.2
Oryza sativa (rice)	Os01g0267200	NP_001042683.1
	PSMD14	XP_001096177.1
Rattus norvegicus (Norway rat)	Psmd14	NP_001020860.1
Kluyveromyces lactis	KLLA0E14147g	XP_454588.1
Homo sapiens (human)	PSMD14	NP_005796.1
Drosophila melanogaster (fruit fly)	Rpn11	NP_608905.1
Schizosaccharomyces pombe (fission yeast)	rpn11	NP_594014.1
Mus musculus (house mouse)	Psmd14	NP_067501.2
Danio rerio (zebrafish)	psmd14	NP_001077042.1
Oryza sativa (rice)	Os05g0371200	NP_001055355.1

Gene Ontology
Bibliography

Related articles in PubMed

Deubiquitinating enzyme PSMD14 promotes tumor metastasis through stabilizing SNAIL in human esophageal squamous cell carcinoma.
Zhu R, Liu Y, Zhou H, Li L, Li Y, Ding F, Cao X, Liu Z
Cancer letters418125-134(2018 Jan)

RPN11 deubiquitinase promotes proliferation and migration of breast cancer cells.
Luo G, Hu N, Xia X, Zhou J, Ye C
Molecular medicine reports16(1)331-338(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deubiquitylase POH1 stabilizes E2F1 protein through binding to and deubiquitylating E2F1 in liver cancer.
Title: POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.

The data demonstrated that proteasomal POH1 is a key de-ubiquitinating enzyme that regulates ubiquitin conjugates generated in response to damage and that several aspects of the DNA double-strand break response are regulated by the proteasome.
Title: The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.

Down-regulation of PSMD14 results in decreased cell proliferation, cell cycle arrest and senescence. A comparative study with PSMB5, revealed that PSMB5 and PSMD14 have different effects on cell cycle, senescence and associated molecular events.
Title: Knockdown of human deubiquitinase PSMD14 induces cell cycle arrest and senescence.

Disorder of POH1 expression is involved in the onset of idiopathic nephrotic syndrome (INS), and confers multidrug resistance in children with INS
Title: Relationship between expression of Pad1 homologue and multidrug resistance of idiopathic nephrotic syndrome.

Specificity for K63-linked polyubiquitin is a common property of the JAMM/MPN+ family of deubiquitinating enzymes.
Title: K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1.

The following PSMD14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMD14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55904	NM_005805.6 Latest version!	Homo sapiens proteasome 26S subunit, non-ATPase 14 (PSMD14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu55904	NM_005805.5	Homo sapiens proteasome 26S subunit, non-ATPase 14 (PSMD14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMD14 ( NM_005805.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005796.1 Homo sapiens proteasome 26S subunit, non-ATPase 14 (PSMD14), mRNA.

Price & Availability↑

CloneID	OHu55904
Clone ID Related Accession (Same CDS sequence)	NM_005805.5 , NM_005805.6
Accession Version	NM_005805.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA133201.1, BC066336.1, U86782.1 and BM978668.1. On Feb 11, 2012 this sequence version replaced NM_005805.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005692.1, AK055128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGAC TTCTTAGACT TGGAGGAGGT ATGCCTGGAC TGGGCCAGGG GCCACCTACA 
GATGCTCCTG CAGTGGACAC AGCAGAACAA GTCTATATCT CTTCCCTGGC ACTGTTAAAA 
ATGTTAAAAC ATGGCCGTGC TGGAGTTCCA ATGGAAGTTA TGGGTTTGAT GCTTGGAGAA 
TTTGTTGATG ATTATACCGT CAGAGTGATT GATGTGTTTG CTATGCCACA GTCAGGAACA 
GGTGTCAGTG TGGAGGCAGT TGATCCAGTG TTCCAAGCTA AAATGTTGGA TATGTTGAAG 
CAGACAGGAA GGCCGGAGAT GGTTGTTGGT TGGTATCACA GTCACCCTGG CTTTGGTTGT 
TGGCTTTCTG GTGTGGATAT CAACACTCAG CAGAGCTTTG AAGCCTTGTC GGAGAGAGCT 
GTGGCAGTGG TTGTGGATCC CATTCAGAGT GTAAAAGGAA AGGTTGTTAT TGATGCCTTC 
AGATTGATCA ATGCTAATAT GATGGTCTTA GGACATGAAC CAAGACAAAC AACTTCGAAT 
CTGGGTCACT TAAACAAGCC ATCTATCCAG GCATTAATTC ATGGACTAAA CAGACATTAT 
TACTCCATTA CTATTAACTA TCGGAAAAAT GAACTGGAAC AGAAGATGTT GCTAAATTTG 
CATAAGAAGA GTTGGATGGA AGGTTTGACA CTTCAGGACT ACAGTGAACA TTGTAAACAC 
AATGAATCAG TGGTAAAAGA GATGTTGGAA TTAGCCAAGA ATTACAATAA GGCTGTAGAA 
GAAGAAGATA AGATGACACC TGAACAGCTG GCAATAAAGA ATGTTGGCAA GCAGGACCCC 
AAACGTCATT TGGAGGAACA TGTGGATGTA CTTATGACCT CAAATATTGT CCAGTGTTTA 
GCAGCTATGT TGGATACTGT CGTATTTAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005796.1
CDS	468..1400
Translation	MDRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDTVVFK

Target ORF information:

RefSeq Version	NM_005805.5
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 14 (PSMD14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005805.5

ORF Insert Sequence:

ATGGACAGAC TTCTTAGACT TGGAGGAGGT ATGCCTGGAC TGGGCCAGGG GCCACCTACA 
GATGCTCCTG CAGTGGACAC AGCAGAACAA GTCTATATCT CTTCCCTGGC ACTGTTAAAA 
ATGTTAAAAC ATGGCCGTGC TGGAGTTCCA ATGGAAGTTA TGGGTTTGAT GCTTGGAGAA 
TTTGTTGATG ATTATACCGT CAGAGTGATT GATGTGTTTG CTATGCCACA GTCAGGAACA 
GGTGTCAGTG TGGAGGCAGT TGATCCAGTG TTCCAAGCTA AAATGTTGGA TATGTTGAAG 
CAGACAGGAA GGCCGGAGAT GGTTGTTGGT TGGTATCACA GTCACCCTGG CTTTGGTTGT 
TGGCTTTCTG GTGTGGATAT CAACACTCAG CAGAGCTTTG AAGCCTTGTC GGAGAGAGCT 
GTGGCAGTGG TTGTGGATCC CATTCAGAGT GTAAAAGGAA AGGTTGTTAT TGATGCCTTC 
AGATTGATCA ATGCTAATAT GATGGTCTTA GGACATGAAC CAAGACAAAC AACTTCGAAT 
CTGGGTCACT TAAACAAGCC ATCTATCCAG GCATTAATTC ATGGACTAAA CAGACATTAT 
TACTCCATTA CTATTAACTA TCGGAAAAAT GAACTGGAAC AGAAGATGTT GCTAAATTTG 
CATAAGAAGA GTTGGATGGA AGGTTTGACA CTTCAGGACT ACAGTGAACA TTGTAAACAC 
AATGAATCAG TGGTAAAAGA GATGTTGGAA TTAGCCAAGA ATTACAATAA GGCTGTAGAA 
GAAGAAGATA AGATGACACC TGAACAGCTG GCAATAAAGA ATGTTGGCAA GCAGGACCCC 
AAACGTCATT TGGAGGAACA TGTGGATGTA CTTATGACCT CAAATATTGT CCAGTGTTTA 
GCAGCTATGT TGGATACTGT CGTATTTAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMD14 ( NM_005805.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005796.1 Homo sapiens proteasome 26S subunit, non-ATPase 14 (PSMD14), mRNA.

Price & Availability↑

CloneID	OHu55904
Clone ID Related Accession (Same CDS sequence)	NM_005805.5 , NM_005805.6
Accession Version	NM_005805.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA133201.1, BC066336.1, U86782.1 and BM978668.1. On Nov 23, 2018 this sequence version replaced NM_005805.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.719900.1, SRR1660807.135555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000409682.8/ ENSP00000386541.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACAGAC TTCTTAGACT TGGAGGAGGT ATGCCTGGAC TGGGCCAGGG GCCACCTACA 
GATGCTCCTG CAGTGGACAC AGCAGAACAA GTCTATATCT CTTCCCTGGC ACTGTTAAAA 
ATGTTAAAAC ATGGCCGTGC TGGAGTTCCA ATGGAAGTTA TGGGTTTGAT GCTTGGAGAA 
TTTGTTGATG ATTATACCGT CAGAGTGATT GATGTGTTTG CTATGCCACA GTCAGGAACA 
GGTGTCAGTG TGGAGGCAGT TGATCCAGTG TTCCAAGCTA AAATGTTGGA TATGTTGAAG 
CAGACAGGAA GGCCGGAGAT GGTTGTTGGT TGGTATCACA GTCACCCTGG CTTTGGTTGT 
TGGCTTTCTG GTGTGGATAT CAACACTCAG CAGAGCTTTG AAGCCTTGTC GGAGAGAGCT 
GTGGCAGTGG TTGTGGATCC CATTCAGAGT GTAAAAGGAA AGGTTGTTAT TGATGCCTTC 
AGATTGATCA ATGCTAATAT GATGGTCTTA GGACATGAAC CAAGACAAAC AACTTCGAAT 
CTGGGTCACT TAAACAAGCC ATCTATCCAG GCATTAATTC ATGGACTAAA CAGACATTAT 
TACTCCATTA CTATTAACTA TCGGAAAAAT GAACTGGAAC AGAAGATGTT GCTAAATTTG 
CATAAGAAGA GTTGGATGGA AGGTTTGACA CTTCAGGACT ACAGTGAACA TTGTAAACAC 
AATGAATCAG TGGTAAAAGA GATGTTGGAA TTAGCCAAGA ATTACAATAA GGCTGTAGAA 
GAAGAAGATA AGATGACACC TGAACAGCTG GCAATAAAGA ATGTTGGCAA GCAGGACCCC 
AAACGTCATT TGGAGGAACA TGTGGATGTA CTTATGACCT CAAATATTGT CCAGTGTTTA 
GCAGCTATGT TGGATACTGT CGTATTTAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005796.1
CDS	318..1250
Translation	MDRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDTVVFK

Target ORF information:

RefSeq Version	NM_005805.6
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 14 (PSMD14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005805.6

ORF Insert Sequence:

ATGGACAGAC TTCTTAGACT TGGAGGAGGT ATGCCTGGAC TGGGCCAGGG GCCACCTACA 
GATGCTCCTG CAGTGGACAC AGCAGAACAA GTCTATATCT CTTCCCTGGC ACTGTTAAAA 
ATGTTAAAAC ATGGCCGTGC TGGAGTTCCA ATGGAAGTTA TGGGTTTGAT GCTTGGAGAA 
TTTGTTGATG ATTATACCGT CAGAGTGATT GATGTGTTTG CTATGCCACA GTCAGGAACA 
GGTGTCAGTG TGGAGGCAGT TGATCCAGTG TTCCAAGCTA AAATGTTGGA TATGTTGAAG 
CAGACAGGAA GGCCGGAGAT GGTTGTTGGT TGGTATCACA GTCACCCTGG CTTTGGTTGT 
TGGCTTTCTG GTGTGGATAT CAACACTCAG CAGAGCTTTG AAGCCTTGTC GGAGAGAGCT 
GTGGCAGTGG TTGTGGATCC CATTCAGAGT GTAAAAGGAA AGGTTGTTAT TGATGCCTTC 
AGATTGATCA ATGCTAATAT GATGGTCTTA GGACATGAAC CAAGACAAAC AACTTCGAAT 
CTGGGTCACT TAAACAAGCC ATCTATCCAG GCATTAATTC ATGGACTAAA CAGACATTAT 
TACTCCATTA CTATTAACTA TCGGAAAAAT GAACTGGAAC AGAAGATGTT GCTAAATTTG 
CATAAGAAGA GTTGGATGGA AGGTTTGACA CTTCAGGACT ACAGTGAACA TTGTAAACAC 
AATGAATCAG TGGTAAAAGA GATGTTGGAA TTAGCCAAGA ATTACAATAA GGCTGTAGAA 
GAAGAAGATA AGATGACACC TGAACAGCTG GCAATAAAGA ATGTTGGCAA GCAGGACCCC 
AAACGTCATT TGGAGGAACA TGTGGATGTA CTTATGACCT CAAATATTGT CCAGTGTTTA 
GCAGCTATGT TGGATACTGT CGTATTTAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deubiquitinating enzyme PSMD14 promotes tumor metastasis through stabilizing SNAIL in human esophageal squamous cell carcinoma.
Cancer letters418125-134(2018 Jan)
Zhu R,Liu Y,Zhou H,Li L,Li Y,Ding F,Cao X,Liu Z

RPN11 deubiquitinase promotes proliferation and migration of breast cancer cells.
Molecular medicine reports16(1)331-338(2017 Jul)
Luo G,Hu N,Xia X,Zhou J,Ye C

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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PSMD14 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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