GNE gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_005476.5	NP_005467.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
NM_001190388.2	NP_001177317.2	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
NM_001374797.1	NP_001361726.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 6
NM_001128227.2	NP_001121699.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
NM_001190388.1	NP_001177317.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
NM_005476.6	NP_005467.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
NM_001374798.1	NP_001361727.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
XM_005251334.4	XP_005251391.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
NM_001190383.1	NP_001177312.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
XM_017014168.1	XP_016869657.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
NM_005476.7	NP_005467.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
NM_001190384.3	NP_001177313.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
NM_001190384.2	NP_001177313.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
NM_001190383.2	NP_001177312.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
NM_001128227.3	NP_001121699.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
NM_001190384.1	NP_001177313.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
NM_001190383.3	NP_001177312.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
XM_017014167.1	XP_016869656.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22152	NM_005476.7 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22850	NM_001128227.3 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22152	XM_017014167.1 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22444	NM_001190384.3 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu22971	NM_001190383.3 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu23071	NM_001190388.1 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu42653	XM_005251334.4 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu108932	NM_001190388.2 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu108932	NM_001374798.1 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu98397	NM_001374797.1 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu98397	XM_017014168.1 Latest version!	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu22152	NM_005476.5	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22152	NM_005476.6	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22444	NM_001190384.2	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu22971	NM_001190383.1	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu22850	NM_001128227.2	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22444	NM_001190384.1	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu22971	NM_001190383.2	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

GNE ( NM_005476.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005467.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22152
Clone ID Related Accession (Same CDS sequence)	NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
Accession Version	NM_005476.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, BC121179.2, AL158830.17 and CA503236.1. On Jun 18, 2010 this sequence version replaced NM_005476.4. Transcript Variant: This variant (2) contains a different 5' terminal exon compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG, and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. This variant has been called GNE1 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.39095.1, SRR1803613.148792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005467.1
CDS	222..2390
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_005476.5
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005476.5

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_005476.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005467.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22152
Clone ID Related Accession (Same CDS sequence)	NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
Accession Version	NM_005476.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, BC121179.2, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2018 this sequence version replaced NM_005476.5. Transcript Variant: This variant (2) contains a different 5' terminal exon compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG, and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. This variant has been called GNE1 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.194578.1, SRR1803617.222976.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005467.1
CDS	171..2339
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_005476.6
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005476.6

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_005476.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_005467.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

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CloneID	OHu22152
Clone ID Related Accession (Same CDS sequence)	NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
Accession Version	NM_005476.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, BC121179.2, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_005476.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.150925.1, SRR1803617.222976.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005467.1
CDS	171..2339
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_005476.7
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005476.7

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001128227.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121699.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.

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CloneID	OHu22850
Clone ID Related Accession (Same CDS sequence)	NM_001128227.2 , NM_001128227.3
Accession Version	NM_001128227.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC418354.1, DC416534.1, AK312539.1, BF197948.1, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jun 18, 2010 this sequence version replaced NM_001128227.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). This variant has been called GNE2 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312539.1, AM697708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG 
CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG 
TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC 
CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC 
ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC 
CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT 
CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA 
ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC 
ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA 
TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT 
CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA 
AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC 
ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC 
CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG 
TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA 
GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT 
CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT 
CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC 
TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC 
CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA 
GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC 
AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT 
CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC 
CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA 
ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC 
CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA 
TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA 
GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG 
GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA 
GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC 
TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT 
GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121699.1
CDS	113..2374
Translation	METYGYLQRESCFQGPHELYFKNLSKRNKQIMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001128227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128227.2

ORF Insert Sequence:

ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG 
CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG 
TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC 
CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC 
ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC 
CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT 
CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA 
ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC 
ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA 
TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT 
CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA 
AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC 
ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC 
CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG 
TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA 
GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT 
CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT 
CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC 
TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC 
CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA 
GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC 
AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT 
CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC 
CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA 
ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC 
CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA 
TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA 
GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG 
GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA 
GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC 
TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT 
GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001128227.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121699.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.

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CloneID	OHu22850
Clone ID Related Accession (Same CDS sequence)	NM_001128227.2 , NM_001128227.3
Accession Version	NM_001128227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC416534.1, AK312539.1, BF197948.1, AL158830.17 and CA503236.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001128227.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312539.1, AM697708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG 
CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG 
TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC 
CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC 
ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC 
CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT 
CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA 
ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC 
ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA 
TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT 
CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA 
AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC 
ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC 
CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG 
TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA 
GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT 
CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT 
CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC 
TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC 
CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA 
GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC 
AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT 
CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC 
CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA 
ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC 
CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA 
TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA 
GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG 
GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA 
GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC 
TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT 
GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121699.1
CDS	35..2296
Translation	METYGYLQRESCFQGPHELYFKNLSKRNKQIMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001128227.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128227.3

ORF Insert Sequence:

ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG 
CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG 
TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC 
CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC 
ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC 
CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT 
CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA 
ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC 
ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA 
TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT 
CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG TGATGATGTA 
AAATCTAAAG ATTACATTGT TGCACTACAG CACCCTGTGA CCACTGACAT TAAGCATTCC 
ATAAAAATGT TTGAATTAAC ATTGGATGCA CTTATCTCAT TTAACAAGCG GACCCTAGTC 
CTGTTTCCAA ATATTGACGC AGGGAGCAAA GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATTGAGCATC ATCCCAACTT TCGTGCAGTT AAACACGTCC CATTTGACCA GTTTATACAG 
TTGGTTGCCC ATGCTGGCTG TATGATTGGG AACAGCAGCT GTGGGGTTCG AGAAGTTGGA 
GCTTTTGGAA CACCTGTGAT CAACCTGGGA ACACGTCAGA TTGGAAGAGA AACAGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTGCAAGC ACTGCACCTT 
CAGTTTGGTA AACAGTACCC TTGTTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTGAAGT TTCTCAAATC TATCGATCTT CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT 
CCTCCTGTGA AGGAGAATAT CTCTCAAGAT ATTGACCATA TTCTTGAAAC TCTAAGTGCC 
TTGGCCGTTG ATCTTGGCGG GACGAACCTC CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTATACTCA GTTCAATCCT AAAACCTATG AAGAGAGGAT TAATTTAATC 
CTACAGATGT GTGTGGAAGC TGCAGCAGAA GCTGTAAAAC TGAACTGCAG AATTTTGGGA 
GTAGGCATTT CCACAGGTGG CCGTGTAAAT CCTCGGGAAG GAATTGTGCT GCATTCAACC 
AAACTGATCC AAGAGTGGAA CTCTGTGGAC CTTAGGACCC CCCTTTCTGA CACTTTGCAT 
CTCCCTGTGT GGGTAGACAA TGATGGCAAC TGTGCTGCCC TGGCGGAAAG GAAATTTGGC 
CAAGGAAAGG GACTGGAAAA CTTTGTTACA CTTATCACAG GCACAGGAAT CGGTGGTGGA 
ATTATCCATC AGCATGAATT GATCCACGGA AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC 
CTTGTTGTGT CTCTGGATGG GCCTGATTGT TCCTGTGGAA GCCATGGGTG CATTGAAGCA 
TACGCCTCTG GAATGGCCTT GCAGAGGGAG GCAAAAAAGC TCCATGATGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAT GAGGCTGTGG GTGCGCTCCA TCTCATCCAA 
GCTGCGAAAC TTGGCAATGC GAAGGCCCAG AGCATCCTAA GAACAGCTGG AACAGCTTTG 
GGTCTTGGGG TTGTGAACAT CCTCCATACC ATGAATCCCT CCCTTGTGAT CCTCTCCGGA 
GTCCTGGCCA GTCACTATAT CCACATTGTC AAAGACGTCA TTCGCCAGCA GGCCTTGTCC 
TCCGTGCAGG ACGTGGATGT GGTGGTTTCG GATTTGGTTG ACCCCGCCCT GCTGGGTGCT 
GCCAGCATGG TTCTGGACTA CACAACACGC AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_017014167.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869656.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

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CloneID	OHu22152
Clone ID Related Accession (Same CDS sequence)	NM_005476.5 , NM_005476.6 , NM_005476.7 , XM_017014167.1
Accession Version	XM_017014167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869656.1
CDS	67..2235
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	XM_017014167.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014167.1

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190384.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177313.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

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CloneID	OHu22444
Clone ID Related Accession (Same CDS sequence)	NM_001190384.3 , NM_001190384.2 , NM_001190384.1
Accession Version	NM_001190384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, AK295562.1, BC121179.2, AL158830.17 and CA503236.1. On Jun 2, 2019 this sequence version replaced NM_001190384.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295562.1, SRR1803611.217709.1 [ECO:0000332] ##Evidence-Data-END##

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA 
CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC 
CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT 
ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC 
GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT 
GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT 
CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC 
CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC 
GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC 
AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177313.1
CDS	142..1980
Translation	MIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190384.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190384.3

ORF Insert Sequence:

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA 
CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC 
CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT 
ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC 
GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT 
GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT 
CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC 
CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC 
GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC 
AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190384.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177313.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22444
Clone ID Related Accession (Same CDS sequence)	NM_001190384.3 , NM_001190384.2 , NM_001190384.1
Accession Version	NM_001190384.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, AK295562.1, BC121179.2, AL158830.17 and CA503236.1. On Jan 20, 2018 this sequence version replaced NM_001190384.1. Transcript Variant: This variant (5) contains a different 5' terminal exon and lacks two alternate 5' coding region segments, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 5) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.217709.1, AK295562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA 
CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC 
CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT 
ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC 
GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT 
GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT 
CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC 
CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC 
GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC 
AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177313.1
CDS	142..1980
Translation	MIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190384.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190384.2

ORF Insert Sequence:

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA 
CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC 
CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT 
ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC 
GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT 
GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT 
CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC 
CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC 
GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC 
AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190384.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177313.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22444
Clone ID Related Accession (Same CDS sequence)	NM_001190384.3 , NM_001190384.2 , NM_001190384.1
Accession Version	NM_001190384.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, AK295562.1, BC121179.2, AL158830.17 and CA503236.1. Transcript Variant: This variant (5) contains a different 5' terminal exon and lacks two alternate 5' coding region segments, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 5) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.217709.1, AK295562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA 
CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC 
CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT 
ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC 
GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT 
GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT 
CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC 
CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC 
GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC 
AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177313.1
CDS	193..2031
Translation	MIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190384.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190384.1

ORF Insert Sequence:

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAACTTTCG TGCAGTTAAA CACGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCCCATG CTGGCTGTAT GATTGGGAAC AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CCTGGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTTCTTCATG TCCGGGATGC TGACACCCAA GACAAAATAT TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT CGATCTTCAA GAGCCACTGC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCCGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TGGAAGCTGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCCA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TTGTGCTGCA TTCAACCAAA 
CTGATCCAAG AGTGGAACTC TGTGGACCTT AGGACCCCCC TTTCTGACAC TTTGCATCTC 
CCTGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT 
ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC 
GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT 
GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT 
CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC 
CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC 
GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC 
AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190383.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177312.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22971
Clone ID Related Accession (Same CDS sequence)	NM_001190383.1 , NM_001190383.2 , NM_001190383.3
Accession Version	NM_001190383.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, EU093084.1, AL158830.17 and CA503236.1. Transcript Variant: This variant (4) contains a different 5' terminal exon and lacks a 3' coding region segment, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 4) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU093084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT 
GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG 
GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG 
TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG 
AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC 
TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG 
GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG 
GACTACACAA CACGCAGGAT CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177312.1
CDS	222..2168
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190383.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190383.1

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT 
GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG 
GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG 
TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG 
AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC 
TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG 
GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG 
GACTACACAA CACGCAGGAT CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190383.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177312.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22971
Clone ID Related Accession (Same CDS sequence)	NM_001190383.1 , NM_001190383.2 , NM_001190383.3
Accession Version	NM_001190383.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, EU093084.1, AL158830.17 and CA503236.1. On Jan 19, 2018 this sequence version replaced NM_001190383.1. Transcript Variant: This variant (4) contains a different 5' terminal exon and lacks a 3' coding region segment, compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG. The predicted protein (isoform 4) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU093084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT 
GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG 
GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG 
TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG 
AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC 
TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG 
GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG 
GACTACACAA CACGCAGGAT CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177312.1
CDS	171..2117
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190383.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190383.2

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT 
GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG 
GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG 
TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG 
AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC 
TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG 
GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG 
GACTACACAA CACGCAGGAT CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190383.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177312.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22971
Clone ID Related Accession (Same CDS sequence)	NM_001190383.1 , NM_001190383.2 , NM_001190383.3
Accession Version	NM_001190383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201585.1, EU093084.1, AL158830.17 and CA503236.1. On Jun 2, 2019 this sequence version replaced NM_001190383.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU093084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT 
GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG 
GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG 
TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG 
AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC 
TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG 
GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG 
GACTACACAA CACGCAGGAT CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177312.1
CDS	171..2117
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190383.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190383.3

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC ACTACAGCAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTCCTG TTTCCAAATA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGAATCGGTG GTGGAATTAT CCATCAGCAT 
GAATTGATCC ACGGAAGCTC CTTCTGTGCT GCAGAACTGG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ATTGTTCCTG TGGAAGCCAT GGGTGCATTG AAGCATACGC CTCTGGAATG 
GCCTTGCAGA GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG 
TCAGTGCCAA AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AATGCGAAGG CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG 
AACATCCTCC ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC 
TATATCCACA TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG 
GATGTGGTGG TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG 
GACTACACAA CACGCAGGAT CTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190388.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177317.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.

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CloneID	OHu23071
Clone ID Related Accession (Same CDS sequence)	NM_001190388.1
Accession Version	NM_001190388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC418354.1, AK296687.1, AM697709.1, AL158830.17 and CA503236.1. Transcript Variant: This variant (3) lacks a 5' coding segment, which results in the use of an upstream alternate start codon, compared to variant 1. The predicted protein (isoform 3) has a shorter and distinct N-terminus when it is compared to isoform 1. This variant has been called GNE3 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296687.1, AM697709.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCTATTG GTGACTGCTC CGTGGCAGCT AAACCAAGAA AACAGCTGCT CTGCTCATTA 
TTTCAAACCA CACTAGGGTA CAGAGCTCGT GCTTCGGGAT GGAAACCTAT GGTTATCTGC 
AGAGGGAGTC ATGCTTTCAA GGACCTCATA AATACATATC GAATGATTGA ACAAGATGAC 
TTTGACATTA ACACCAGGCT ACACACAATT GTGAGGGGAG AAGATGAGGC AGCCATGGTG 
GAGTCAGTAG GCCTGGCCCT AGTGAAGCTG CCAGATGTCC TTAATCGCCT GAAGCCTGAT 
ATCATGATTG TTCATGGAGA CAGGTTTGAT GCCCTGGCTC TGGCCACATC TGCTGCCTTG 
ATGAACATCC GAATCCTTCA CATTGAAGGT GGGGAAGTCA GTGGGACCAT TGATGACTCT 
ATCAGACATG CCATAACAAA ACTGGCTCAT TATCATGTGT GCTGCACCCG CAGTGCAGAG 
CAGCACCTGA TATCCATGTG TGAGGACCAT GATCGCATCC TTTTGGCAGG CTGCCCTTCC 
TATGACAAAC TTCTCTCAGC CAAGAACAAA GACTACATGA GCATCATTCG CATGTGGCTA 
GGTGATGATG TAAAATCTAA AGATTACATT GTTGCACTAC AGCACCCTGT GACCACTGAC 
ATTAAGCATT CCATAAAAAT GTTTGAATTA ACATTGGATG CACTTATCTC ATTTAACAAG 
CGGACCCTAG TCCTGTTTCC AAATATTGAC GCAGGGAGCA AAGAGATGGT TCGAGTGATG 
CGGAAGAAGG GCATTGAGCA TCATCCCAAC TTTCGTGCAG TTAAACACGT CCCATTTGAC 
CAGTTTATAC AGTTGGTTGC CCATGCTGGC TGTATGATTG GGAACAGCAG CTGTGGGGTT 
CGAGAAGTTG GAGCTTTTGG AACACCTGTG ATCAACCTGG GAACACGTCA GATTGGAAGA 
GAAACAGGGG AGAATGTTCT TCATGTCCGG GATGCTGACA CCCAAGACAA AATATTGCAA 
GCACTGCACC TTCAGTTTGG TAAACAGTAC CCTTGTTCAA AGATATATGG GGATGGAAAT 
GCTGTTCCAA GGATTTTGAA GTTTCTCAAA TCTATCGATC TTCAAGAGCC ACTGCAAAAG 
AAATTCTGCT TTCCTCCTGT GAAGGAGAAT ATCTCTCAAG ATATTGACCA TATTCTTGAA 
ACTCTAAGTG CCTTGGCCGT TGATCTTGGC GGGACGAACC TCCGAGTTGC AATAGTCAGC 
ATGAAGGGTG AAATAGTTAA GAAGTATACT CAGTTCAATC CTAAAACCTA TGAAGAGAGG 
ATTAATTTAA TCCTACAGAT GTGTGTGGAA GCTGCAGCAG AAGCTGTAAA ACTGAACTGC 
AGAATTTTGG GAGTAGGCAT TTCCACAGGT GGCCGTGTAA ATCCTCGGGA AGGAATTGTG 
CTGCATTCAA CCAAACTGAT CCAAGAGTGG AACTCTGTGG ACCTTAGGAC CCCCCTTTCT 
GACACTTTGC ATCTCCCTGT GTGGGTAGAC AATGATGGCA ACTGTGCTGC CCTGGCGGAA 
AGGAAATTTG GCCAAGGAAA GGGACTGGAA AACTTTGTTA CACTTATCAC AGGCACAGGA 
ATCGGTGGTG GAATTATCCA TCAGCATGAA TTGATCCACG GAAGCTCCTT CTGTGCTGCA 
GAACTGGGCC ACCTTGTTGT GTCTCTGGAT GGGCCTGATT GTTCCTGTGG AAGCCATGGG 
TGCATTGAAG CATACGCCTC TGGAATGGCC TTGCAGAGGG AGGCAAAAAA GCTCCATGAT 
GAGGACCTGC TCTTGGTGGA AGGGATGTCA GTGCCAAAAG ATGAGGCTGT GGGTGCGCTC 
CATCTCATCC AAGCTGCGAA ACTTGGCAAT GCGAAGGCCC AGAGCATCCT AAGAACAGCT 
GGAACAGCTT TGGGTCTTGG GGTTGTGAAC ATCCTCCATA CCATGAATCC CTCCCTTGTG 
ATCCTCTCCG GAGTCCTGGC CAGTCACTAT ATCCACATTG TCAAAGACGT CATTCGCCAG 
CAGGCCTTGT CCTCCGTGCA GGACGTGGAT GTGGTGGTTT CGGATTTGGT TGACCCCGCC 
CTGCTGGGTG CTGCCAGCAT GGTTCTGGAC TACACAACAC GCAGGATCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177317.1
CDS	15..2168
Translation	MPIGDCSVAAKPRKQLLCSLFQTTLGYRARASGWKPMVICRGSHAFKDLINTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190388.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190388.1

ORF Insert Sequence:

ATGCCTATTG GTGACTGCTC CGTGGCAGCT AAACCAAGAA AACAGCTGCT CTGCTCATTA 
TTTCAAACCA CACTAGGGTA CAGAGCTCGT GCTTCGGGAT GGAAACCTAT GGTTATCTGC 
AGAGGGAGTC ATGCTTTCAA GGACCTCATA AATACATATC GAATGATTGA ACAAGATGAC 
TTTGACATTA ACACCAGGCT ACACACAATT GTGAGGGGAG AAGATGAGGC AGCCATGGTG 
GAGTCAGTAG GCCTGGCCCT AGTGAAGCTG CCAGATGTCC TTAATCGCCT GAAGCCTGAT 
ATCATGATTG TTCATGGAGA CAGGTTTGAT GCCCTGGCTC TGGCCACATC TGCTGCCTTG 
ATGAACATCC GAATCCTTCA CATTGAAGGT GGGGAAGTCA GTGGGACCAT TGATGACTCT 
ATCAGACATG CCATAACAAA ACTGGCTCAT TATCATGTGT GCTGCACCCG CAGTGCAGAG 
CAGCACCTGA TATCCATGTG TGAGGACCAT GATCGCATCC TTTTGGCAGG CTGCCCTTCC 
TATGACAAAC TTCTCTCAGC CAAGAACAAA GACTACATGA GCATCATTCG CATGTGGCTA 
GGTGATGATG TAAAATCTAA AGATTACATT GTTGCACTAC AGCACCCTGT GACCACTGAC 
ATTAAGCATT CCATAAAAAT GTTTGAATTA ACATTGGATG CACTTATCTC ATTTAACAAG 
CGGACCCTAG TCCTGTTTCC AAATATTGAC GCAGGGAGCA AAGAGATGGT TCGAGTGATG 
CGGAAGAAGG GCATTGAGCA TCATCCCAAC TTTCGTGCAG TTAAACACGT CCCATTTGAC 
CAGTTTATAC AGTTGGTTGC CCATGCTGGC TGTATGATTG GGAACAGCAG CTGTGGGGTT 
CGAGAAGTTG GAGCTTTTGG AACACCTGTG ATCAACCTGG GAACACGTCA GATTGGAAGA 
GAAACAGGGG AGAATGTTCT TCATGTCCGG GATGCTGACA CCCAAGACAA AATATTGCAA 
GCACTGCACC TTCAGTTTGG TAAACAGTAC CCTTGTTCAA AGATATATGG GGATGGAAAT 
GCTGTTCCAA GGATTTTGAA GTTTCTCAAA TCTATCGATC TTCAAGAGCC ACTGCAAAAG 
AAATTCTGCT TTCCTCCTGT GAAGGAGAAT ATCTCTCAAG ATATTGACCA TATTCTTGAA 
ACTCTAAGTG CCTTGGCCGT TGATCTTGGC GGGACGAACC TCCGAGTTGC AATAGTCAGC 
ATGAAGGGTG AAATAGTTAA GAAGTATACT CAGTTCAATC CTAAAACCTA TGAAGAGAGG 
ATTAATTTAA TCCTACAGAT GTGTGTGGAA GCTGCAGCAG AAGCTGTAAA ACTGAACTGC 
AGAATTTTGG GAGTAGGCAT TTCCACAGGT GGCCGTGTAA ATCCTCGGGA AGGAATTGTG 
CTGCATTCAA CCAAACTGAT CCAAGAGTGG AACTCTGTGG ACCTTAGGAC CCCCCTTTCT 
GACACTTTGC ATCTCCCTGT GTGGGTAGAC AATGATGGCA ACTGTGCTGC CCTGGCGGAA 
AGGAAATTTG GCCAAGGAAA GGGACTGGAA AACTTTGTTA CACTTATCAC AGGCACAGGA 
ATCGGTGGTG GAATTATCCA TCAGCATGAA TTGATCCACG GAAGCTCCTT CTGTGCTGCA 
GAACTGGGCC ACCTTGTTGT GTCTCTGGAT GGGCCTGATT GTTCCTGTGG AAGCCATGGG 
TGCATTGAAG CATACGCCTC TGGAATGGCC TTGCAGAGGG AGGCAAAAAA GCTCCATGAT 
GAGGACCTGC TCTTGGTGGA AGGGATGTCA GTGCCAAAAG ATGAGGCTGT GGGTGCGCTC 
CATCTCATCC AAGCTGCGAA ACTTGGCAAT GCGAAGGCCC AGAGCATCCT AAGAACAGCT 
GGAACAGCTT TGGGTCTTGG GGTTGTGAAC ATCCTCCATA CCATGAATCC CTCCCTTGTG 
ATCCTCTCCG GAGTCCTGGC CAGTCACTAT ATCCACATTG TCAAAGACGT CATTCGCCAG 
CAGGCCTTGT CCTCCGTGCA GGACGTGGAT GTGGTGGTTT CGGATTTGGT TGACCCCGCC 
CTGCTGGGTG CTGCCAGCAT GGTTCTGGAC TACACAACAC GCAGGATCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_005251334.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251391.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

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CloneID	OHu42653
Clone ID Related Accession (Same CDS sequence)	XM_005251334.4
Accession Version	XM_005251334.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG 
CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG 
TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC 
CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC 
ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC 
CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT 
CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA 
ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC 
ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA 
TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT 
CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251391.1
CDS	113..2221
Translation	METYGYLQRESCFQGPHELYFKNLSKRNKQIMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	XM_005251334.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251334.4

ORF Insert Sequence:

ATGGAAACCT ATGGTTATCT GCAGAGGGAG TCATGCTTTC AAGGACCTCA TGAACTCTAT 
TTTAAGAACC TCTCAAAACG AAACAAGCAA ATCATGGAGA AGAATGGAAA TAACCGAAAG 
CTGCGGGTTT GTGTTGCTAC TTGTAACCGT GCAGATTATT CTAAACTTGC CCCGATCATG 
TTTGGCATTA AAACCGAACC TGAGTTCTTT GAACTTGATG TTGTGGTACT TGGCTCTCAC 
CTGATAGATG ACTATGGAAA TACATATCGA ATGATTGAAC AAGATGACTT TGACATTAAC 
ACCAGGCTAC ACACAATTGT GAGGGGAGAA GATGAGGCAG CCATGGTGGA GTCAGTAGGC 
CTGGCCCTAG TGAAGCTGCC AGATGTCCTT AATCGCCTGA AGCCTGATAT CATGATTGTT 
CATGGAGACA GGTTTGATGC CCTGGCTCTG GCCACATCTG CTGCCTTGAT GAACATCCGA 
ATCCTTCACA TTGAAGGTGG GGAAGTCAGT GGGACCATTG ATGACTCTAT CAGACATGCC 
ATAACAAAAC TGGCTCATTA TCATGTGTGC TGCACCCGCA GTGCAGAGCA GCACCTGATA 
TCCATGTGTG AGGACCATGA TCGCATCCTT TTGGCAGGCT GCCCTTCCTA TGACAAACTT 
CTCTCAGCCA AGAACAAAGA CTACATGAGC ATCATTCGCA TGTGGCTAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAACTTTCG TGCAGTTAAA 
CACGTCCCAT TTGACCAGTT TATACAGTTG GTTGCCCATG CTGGCTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTTCGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CCTGGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TGCAAGCACT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT CGATCTTCAA 
GAGCCACTGC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCCGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TGGAAGCTGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCCA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TTGTGCTGCA TTCAACCAAA CTGATCCAAG AGTGGAACTC TGTGGACCTT 
AGGACCCCCC TTTCTGACAC TTTGCATCTC CCTGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGAC TGGAAAACTT TGTTACACTT 
ATCACAGGCA CAGGAATCGG TGGTGGAATT ATCCATCAGC ATGAATTGAT CCACGGAAGC 
TCCTTCTGTG CTGCAGAACT GGGCCACCTT GTTGTGTCTC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCATAC GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA 
AAAAAGCTCC ATGATGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAG 
GCTGTGGGTG CGCTCCATCT CATCCAAGCT GCGAAACTTG GCAATGCGAA GGCCCAGAGC 
ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT CTTGGGGTTG TGAACATCCT CCATACCATG 
AATCCCTCCC TTGTGATCCT CTCCGGAGTC CTGGCCAGTC ACTATATCCA CATTGTCAAA 
GACGTCATTC GCCAGCAGGC CTTGTCCTCC GTGCAGGACG TGGATGTGGT GGTTTCGGAT 
TTGGTTGACC CCGCCCTGCT GGGTGCTGCC AGCATGGTTC TGGACTACAC AACACGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001190388.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177317.2 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108932
Clone ID Related Accession (Same CDS sequence)	NM_001190388.2 , NM_001374798.1
Accession Version	NM_001190388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158830.17. On Oct 16, 2019 this sequence version replaced NM_001190388.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296687.1, AM697709.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG 
CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA 
CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA 
GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT 
AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG 
AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA 
ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC 
CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG 
ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT 
CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT 
ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC 
CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT 
AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA 
GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT 
CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC 
CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC 
TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA 
CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA 
AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT 
TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG 
GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT 
GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG 
AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC 
ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC 
AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG 
GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC 
AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177317.2
CDS	99..2090
Translation	MIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001190388.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190388.2

ORF Insert Sequence:

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG 
CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA 
CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA 
GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT 
AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG 
AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA 
ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC 
CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG 
ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT 
CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT 
ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC 
CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT 
AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA 
GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT 
CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC 
CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC 
TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA 
CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA 
AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT 
TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG 
GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT 
GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG 
AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC 
ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC 
AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG 
GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC 
AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001374798.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361727.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu108932
Clone ID Related Accession (Same CDS sequence)	NM_001190388.2 , NM_001374798.1
Accession Version	NM_001374798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158830.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.164519.1, SRR1803617.124877.1 [ECO:0000332] ##Evidence-Data-END##

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG 
CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA 
CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA 
GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT 
AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG 
AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA 
ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC 
CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG 
ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT 
CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT 
ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC 
CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT 
AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA 
GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT 
CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC 
CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC 
TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA 
CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA 
AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT 
TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG 
GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT 
GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG 
AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC 
ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC 
AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG 
GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC 
AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361727.1
CDS	142..2133
Translation	MIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001374798.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374798.1

ORF Insert Sequence:

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGCTAC ACACAATTGT GAGGGGAGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTAG TGAAGCTGCC AGATGTCCTT 
AATCGCCTGA AGCCTGATAT CATGATTGTT CATGGAGACA GGTTTGATGC CCTGGCTCTG 
GCCACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGTGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACATGCC ATAACAAAAC TGGCTCATTA TCATGTGTGC 
TGCACCCGCA GTGCAGAGCA GCACCTGATA TCCATGTGTG AGGACCATGA TCGCATCCTT 
TTGGCAGGCT GCCCTTCCTA TGACAAACTT CTCTCAGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGCA TGTGGCTAGG TGATGATGTA AAATCTAAAG ATTACATTGT TGCACTACAG 
CACCCTGTGA CCACTGACAT TAAGCATTCC ATAAAAATGT TTGAATTAAC ATTGGATGCA 
CTTATCTCAT TTAACAAGCG GACCCTAGTC CTGTTTCCAA ATATTGACGC AGGGAGCAAA 
GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAACTT TCGTGCAGTT 
AAACACGTCC CATTTGACCA GTTTATACAG TTGGTTGCCC ATGCTGGCTG TATGATTGGG 
AACAGCAGCT GTGGGGTTCG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACCTGGGA 
ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTTCTTC ATGTCCGGGA TGCTGACACC 
CAAGACAAAA TATTGCAAGC ACTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG 
ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATCGATCTT 
CAAGAGCCAC TGCAAAAGAA ATTCTGCTTT CCTCCTGTGA AGGAGAATAT CTCTCAAGAT 
ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCCGTTG ATCTTGGCGG GACGAACCTC 
CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT 
AAAACCTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTGGAAGC TGCAGCAGAA 
GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CCACAGGTGG CCGTGTAAAT 
CCTCGGGAAG GAATTGTGCT GCATTCAACC AAACTGATCC AAGAGTGGAA CTCTGTGGAC 
CTTAGGACCC CCCTTTCTGA CACTTTGCAT CTCCCTGTGT GGGTAGACAA TGATGGCAAC 
TGTGCTGCCC TGGCGGAAAG GAAATTTGGC CAAGGAAAGG GACTGGAAAA CTTTGTTACA 
CTTATCACAG GCACAGGAAT CGGTGGTGGA ATTATCCATC AGCATGAATT GATCCACGGA 
AGCTCCTTCT GTGCTGCAGA ACTGGGCCAC CTTGTTGTGT CTCTGGATGG GCCTGATTGT 
TCCTGTGGAA GCCATGGGTG CATTGAAGCA TACGCCTCTG GAATGGCCTT GCAGAGGGAG 
GCAAAAAAGC TCCATGATGA GGACCTGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT 
GAGGCTGTGG GTGCGCTCCA TCTCATCCAA GCTGCGAAAC TTGGCAATGC GAAGGCCCAG 
AGCATCCTAA GAACAGCTGG AACAGCTTTG GGTCTTGGGG TTGTGAACAT CCTCCATACC 
ATGAATCCCT CCCTTGTGAT CCTCTCCGGA GTCCTGGCCA GTCACTATAT CCACATTGTC 
AAAGACGTCA TTCGCCAGCA GGCCTTGTCC TCCGTGCAGG ACGTGGATGT GGTGGTTTCG 
GATTTGGTTG ACCCCGCCCT GCTGGGTGCT GCCAGCATGG TTCTGGACTA CACAACACGC 
AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( NM_001374797.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361726.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu98397
Clone ID Related Accession (Same CDS sequence)	NM_001374797.1 , XM_017014168.1
Accession Version	NM_001374797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158830.17. On Oct 16, 2019 this sequence version replaced XM_017014168.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.111094.1, SRR1803613.74655.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG 
CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT 
GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT 
GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT 
CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT 
GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG 
AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT 
GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC 
GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT 
AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG 
ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC 
ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG 
ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT 
GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA 
AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC 
CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT 
GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC 
TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG 
GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG 
AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT 
GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG 
GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG 
CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC 
ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361726.1
CDS	171..2186
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	NM_001374797.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374797.1

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG 
CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT 
GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT 
GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT 
CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT 
GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG 
AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT 
GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC 
GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT 
AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG 
ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC 
ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG 
ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT 
GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA 
AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC 
CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT 
GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC 
TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG 
GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG 
AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT 
GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG 
GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG 
CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC 
ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_017014168.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869657.1 Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

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CloneID	OHu98397
Clone ID Related Accession (Same CDS sequence)	NM_001374797.1 , XM_017014168.1
Accession Version	XM_017014168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG 
CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT 
GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT 
GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT 
CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT 
GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG 
AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT 
GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC 
GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT 
AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG 
ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC 
ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG 
ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT 
GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA 
AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC 
CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT 
GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC 
TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG 
GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG 
AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT 
GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG 
GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG 
CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC 
ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869657.1
CDS	199..2214
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	XM_017014168.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014168.1

ORF Insert Sequence:

ATGGAGAAGA ATGGAAATAA CCGAAAGCTG CGGGTTTGTG TTGCTACTTG TAACCGTGCA 
GATTATTCTA AACTTGCCCC GATCATGTTT GGCATTAAAA CCGAACCTGA GTTCTTTGAA 
CTTGATGTTG TGGTACTTGG CTCTCACCTG ATAGATGACT ATGGAAATAC ATATCGAATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGCTACACA CAATTGTGAG GGGAGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTAGTGA AGCTGCCAGA TGTCCTTAAT 
CGCCTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGATGCCCT GGCTCTGGCC 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACATGCCATA ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGCAGTG CAGAGCAGCA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTTTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCAGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGCATGT GGCTAGGGAG CAAAGAGATG GTTCGAGTGA TGCGGAAGAA GGGCATTGAG 
CATCATCCCA ACTTTCGTGC AGTTAAACAC GTCCCATTTG ACCAGTTTAT ACAGTTGGTT 
GCCCATGCTG GCTGTATGAT TGGGAACAGC AGCTGTGGGG TTCGAGAAGT TGGAGCTTTT 
GGAACACCTG TGATCAACCT GGGAACACGT CAGATTGGAA GAGAAACAGG GGAGAATGTT 
CTTCATGTCC GGGATGCTGA CACCCAAGAC AAAATATTGC AAGCACTGCA CCTTCAGTTT 
GGTAAACAGT ACCCTTGTTC AAAGATATAT GGGGATGGAA ATGCTGTTCC AAGGATTTTG 
AAGTTTCTCA AATCTATCGA TCTTCAAGAG CCACTGCAAA AGAAATTCTG CTTTCCTCCT 
GTGAAGGAGA ATATCTCTCA AGATATTGAC CATATTCTTG AAACTCTAAG TGCCTTGGCC 
GTTGATCTTG GCGGGACGAA CCTCCGAGTT GCAATAGTCA GCATGAAGGG TGAAATAGTT 
AAGAAGTATA CTCAGTTCAA TCCTAAAACC TATGAAGAGA GGATTAATTT AATCCTACAG 
ATGTGTGTGG AAGCTGCAGC AGAAGCTGTA AAACTGAACT GCAGAATTTT GGGAGTAGGC 
ATTTCCACAG GTGGCCGTGT AAATCCTCGG GAAGGAATTG TGCTGCATTC AACCAAACTG 
ATCCAAGAGT GGAACTCTGT GGACCTTAGG ACCCCCCTTT CTGACACTTT GCATCTCCCT 
GTGTGGGTAG ACAATGATGG CAACTGTGCT GCCCTGGCGG AAAGGAAATT TGGCCAAGGA 
AAGGGACTGG AAAACTTTGT TACACTTATC ACAGGCACAG GAATCGGTGG TGGAATTATC 
CATCAGCATG AATTGATCCA CGGAAGCTCC TTCTGTGCTG CAGAACTGGG CCACCTTGTT 
GTGTCTCTGG ATGGGCCTGA TTGTTCCTGT GGAAGCCATG GGTGCATTGA AGCATACGCC 
TCTGGAATGG CCTTGCAGAG GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG 
GAAGGGATGT CAGTGCCAAA AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG 
AAACTTGGCA ATGCGAAGGC CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT 
GGGGTTGTGA ACATCCTCCA TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG 
GCCAGTCACT ATATCCACAT TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG 
CAGGACGTGG ATGTGGTGGT TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC 
ATGGTTCTGG ACTACACAAC ACGCAGGATC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GNE
Entrez Gene ID	10020
Full Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Synonyms	DMRV,GLCNE,IBM2,NM,Uae1
General protein information	Preferred Names glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase Names bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase N-acylmannosamine kinase UDP-GlcNAc-2-epimerase/ManAc kinase UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p13.3
Summary	The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	603824
Disorder Html:	Sialuria, 269921 (3); Nonaka myopathy, 605820 (3)

Homo sapiens (human)	GNE	NP_001121699.1
Mus musculus (house mouse)	Gne	NP_056643.3
Rattus norvegicus (Norway rat)	Gne	NP_446217.1
	GNE	XP_001082113.2
Gallus gallus (chicken)	GNE	NP_001026603.2
Xenopus tropicalis (tropical clawed frog)	gne	NP_001072728.1
Canis lupus familiaris (dog)	GNE	XP_003431623.1
Danio rerio (zebrafish)	gne	NP_957177.1
Pan troglodytes (chimpanzee)	GNE	XP_003312121.1
Bos taurus (cattle)	GNE	NP_001178072.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GNE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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