LOC100784758 cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100784758
Entrez Gene ID	100784758
Full Name	adoMet-dependent rRNA methyltransferase spb1-like
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003540630.3	XP_003540678.1	adoMet-dependent rRNA methyltransferase spb1

Pathways from BioSystems
Gene Ontology

The following LOC100784758 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100784758 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl30839	XM_003540630.3 Latest version!	Glycine max adoMet-dependent rRNA methyltransferase spb1 (LOC100784758), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100784758 ( XM_003540630.3 ) cDNA ORF clone, Glycine max(soybean) -> XP_003540678.1 Glycine max adoMet-dependent rRNA methyltransferase spb1 (LOC100784758), mRNA.

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CloneID	OGl30839
Clone ID Related Accession (Same CDS sequence)	XM_003540630.3
Accession Version	XM_003540630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-30
Organism	Glycine max(soybean)
Product	adoMet-dependent rRNA methyltransferase spb1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_038248.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 25, 2015 this sequence version replaced XM_003540630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Glycine max Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG CGAAGGGGAA GCACCGTTTG GACAAGTATT ACCACTTAGC GAAAGAGCAC 
GGTTACAGGT CTCGAGCCTC ATGGAAGCTG GTCCAGCTCA ACTCCAAGTT CGGCTTCCTG 
GAATCGGCCC GCGCCGTGCT GGACCTCTGC GCCGCCCCCG GAGGGTGGAT GCAGGTGGTG 
GTCCAGCGCG TACCCGTGGA CCACCTGGTG ATCGGCGTGG ACCTCGCCCC CATCGCCCCC 
GTCCGTGGCG CCATCGCCAT CCAGGAAGAC ATCACCAGGC CCGAGTGCAA GTCCCGCATC 
AAGAAGCTCA TGAACGACCA CGGCTGCCGC GCCTTCGACG TCATCTTGCA CGACGGTTCC 
CCCAACGTCG GTGGCGCCTG GGCCCAGGAA GCTATGAGCC AGAACGCCCT CGTTATTGAT 
GCTGTCAAGT TGGCCACTCA GTTCTTAGCC CCCAAGGGCA AGTTTGTAAC CAAAATTTTC 
AGGTCGCAAG ATTACAGTTC TGTGGTGTAT TGTTTGAAGC AGTTGTTTGA GAAGGTTGAG 
GTGGATAAGC CTGCGGCTAG TCGTTCTGAA TCAGCTGAGA TATATGTTTT GGGGCTTGGG 
TATAAGGCCC CTGCTAAGAT TGATCCACGG CTTCTTGATG TGAAGCATCT CTTTCAGGGA 
TCTGTGGAAC CACAGCCTAA GGTGGTGGAT GTACTTCGAG ATACTAAGCA AAAAAGGCAC 
CGTGATGGCT ATGAAGATGG AAACACAACT TTGAGGAAGG TGTCTTCAGC TGCAAATTTC 
ATTTGGTCAA ATTCTCCTCT GGAGATCCTT GGTTCAGTCA CTTCCATAAC CTTTACTGAT 
CCAGCTGATT CACTGATCAA GGATCATGAC CTAACTAGTG AAGAGGTAAA ATCTCTCTGC 
GATGATTTAA GGGTTCTGGG GAAGCAAGAC TTCAAGCATC TTCTAAAGTG GAGGATTCAA 
GTTAGAAAAG CTCTTTCACC AACTCAAAAG CCTGATTCTA CCACTACAGA ACTGATGGAC 
AATGAGCCTA AGGTGGTGGA TGAAGAAGAT AGAATACTCA ATGAAATGGA GGAACTGACT 
TATGTAATGG ACCGCAAGAA GAAACGTGCA AAGAAGCTTC TTGCAAAAAG AAGAGCCAAG 
GACAAGGCAC GAAAAGCAAC AGGGATGCAA ATGGATGCAA TAGATGATGG TTATGTAGAT 
CAAGAGTTGT TTGCACTTTC CTCTATTAAG GGTAAGAAGG ATCTGGTAGC TGTTGACAAC 
ACTGAGTATG AAGGTGATGA AGGAGAAGTA GAAGACAGTG AGAATGAAGA AACCCATGAA 
GGCCGCCCAG AGCATTCATC CAGTGACTTG GAAGATTCTG ATGAGGAACG TAAAAGATAC 
AATGAACAAA TGGAAGATTT GATGGATCAA GCTTACGAGA GGTTTGTGAT CAGAAAGGAA 
GGAAGTGCAA AACAGAGGAA GCGAATTAAA AAATCCTATG ATGCAAAGGC CCAACTTTTG 
GAGGGTGGTG AGGATGATGA TATTGTTCAA TCCAAGTATG ATTCAGATGA AGATCAGGGT 
GATCAAGAAG CAAATCCATT GATGGTGCCA CTTAATGATG AGGCAGAGCT CACCCAGGAG 
GAGATCATGA ATAAGTGGTT TAGTCAGGAT GTCTTTGCTG AAGCTGCAGA GGAAGGAGAC 
TTTAAGAAGG ATGAGAGTAA AGATGAAATG GATATAGATG AGCCTAAGGA GAAGATATCC 
ATTGCCAAAA AGGTAAAAGA AAATAAAACA GCAGCCCCAG CTGTAGCAAC TCACCCTCAG 
CCTCAACCAT CCAAGGCAGG GGATGATTTT GAAATAGTTC CTGCACCCGA TACTGATTCA 
AGTGATGATT CATCCTCTGA TGAATGGGAA GAAGATATTG AAGCCAAAGC TGAGATATTG 
GCTTATGCTA AGAAGATGAT GAGGAAAAAG CAACGGGAGC ACTTACTGGA TGATGCATAC 
AACAAGTACA TGTTTGATGA CGAAGGATTG CCCAAGTGGT TTCTAGATGA GGAGAGGAGA 
CATCGCCAAC CAATAAAGCC TATTACCAAA GAGGAAATTG CTGCGATGAA AGCACAGTTT 
AAAGAAATTG ATGCTCGACC TGCGAAGAAG GTGGCAGAGG CCAAAGCACG GAAGAAGCGT 
GTTGCAATGA GGAAACTTGA GAAGGTACGG AAGAAGGCCA ATGCCATATC AGATCAGACA 
GAGATATCTG ATCGTTCGAA GAGGAAGCAA ATTGAACAAC TATATAAAAG GGCTGTTCCT 
AAGAGGCCTA AAAAGGAATA TGTAGTGGCA AAGAAAGGTG TCCAGGTAAG GGCTGGCAAG 
GGAAAAGTCC TTGTTGATCG CAGAATGAAG AAGGATGCTA GGAAACATGG ATCGGGTAAG 
GGTGGAAAAG GAGGTTCTAA GACGAAGGGC AAGGCTCCAA AGGGCAAAGG AGGATCTTCA 
AAGGCCTCTG CCTCTGCAAA AAAAGGGAAA CAGAGAACTA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003540678.1
CDS	60..2564
Translation	MGKAKGKHRLDKYYHLAKEHGYRSRASWKLVQLNSKFGFLESARAVLDLCAAPGGWMQVVVQRVPVDHLVIGVDLAPIAPVRGAIAIQEDITRPECKSRIKKLMNDHGCRAFDVILHDGSPNVGGAWAQEAMSQNALVIDAVKLATQFLAPKGKFVTKIFRSQDYSSVVYCLKQLFEKVEVDKPAASRSESAEIYVLGLGYKAPAKIDPRLLDVKHLFQGSVEPQPKVVDVLRDTKQKRHRDGYEDGNTTLRKVSSAANFIWSNSPLEILGSVTSITFTDPADSLIKDHDLTSEEVKSLCDDLRVLGKQDFKHLLKWRIQVRKALSPTQKPDSTTTELMDNEPKVVDEEDRILNEMEELTYVMDRKKKRAKKLLAKRRAKDKARKATGMQMDAIDDGYVDQELFALSSIKGKKDLVAVDNTEYEGDEGEVEDSENEETHEGRPEHSSSDLEDSDEERKRYNEQMEDLMDQAYERFVIRKEGSAKQRKRIKKSYDAKAQLLEGGEDDDIVQSKYDSDEDQGDQEANPLMVPLNDEAELTQEEIMNKWFSQDVFAEAAEEGDFKKDESKDEMDIDEPKEKISIAKKVKENKTAAPAVATHPQPQPSKAGDDFEIVPAPDTDSSDDSSSDEWEEDIEAKAEILAYAKKMMRKKQREHLLDDAYNKYMFDDEGLPKWFLDEERRHRQPIKPITKEEIAAMKAQFKEIDARPAKKVAEAKARKKRVAMRKLEKVRKKANAISDQTEISDRSKRKQIEQLYKRAVPKRPKKEYVVAKKGVQVRAGKGKVLVDRRMKKDARKHGSGKGGKGGSKTKGKAPKGKGGSSKASASAKKGKQRTK

Target ORF information:

RefSeq Version	XM_003540630.3
Organism	Glycine max(soybean)
Definition	Glycine max adoMet-dependent rRNA methyltransferase spb1 (LOC100784758), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003540630.3

ORF Insert Sequence:

ATGGGGAAGG CGAAGGGGAA GCACCGTTTG GACAAGTATT ACCACTTAGC GAAAGAGCAC 
GGTTACAGGT CTCGAGCCTC ATGGAAGCTG GTCCAGCTCA ACTCCAAGTT CGGCTTCCTG 
GAATCGGCCC GCGCCGTGCT GGACCTCTGC GCCGCCCCCG GAGGGTGGAT GCAGGTGGTG 
GTCCAGCGCG TACCCGTGGA CCACCTGGTG ATCGGCGTGG ACCTCGCCCC CATCGCCCCC 
GTCCGTGGCG CCATCGCCAT CCAGGAAGAC ATCACCAGGC CCGAGTGCAA GTCCCGCATC 
AAGAAGCTCA TGAACGACCA CGGCTGCCGC GCCTTCGACG TCATCTTGCA CGACGGTTCC 
CCCAACGTCG GTGGCGCCTG GGCCCAGGAA GCTATGAGCC AGAACGCCCT CGTTATTGAT 
GCTGTCAAGT TGGCCACTCA GTTCTTAGCC CCCAAGGGCA AGTTTGTAAC CAAAATTTTC 
AGGTCGCAAG ATTACAGTTC TGTGGTGTAT TGTTTGAAGC AGTTGTTTGA GAAGGTTGAG 
GTGGATAAGC CTGCGGCTAG TCGTTCTGAA TCAGCTGAGA TATATGTTTT GGGGCTTGGG 
TATAAGGCCC CTGCTAAGAT TGATCCACGG CTTCTTGATG TGAAGCATCT CTTTCAGGGA 
TCTGTGGAAC CACAGCCTAA GGTGGTGGAT GTACTTCGAG ATACTAAGCA AAAAAGGCAC 
CGTGATGGCT ATGAAGATGG AAACACAACT TTGAGGAAGG TGTCTTCAGC TGCAAATTTC 
ATTTGGTCAA ATTCTCCTCT GGAGATCCTT GGTTCAGTCA CTTCCATAAC CTTTACTGAT 
CCAGCTGATT CACTGATCAA GGATCATGAC CTAACTAGTG AAGAGGTAAA ATCTCTCTGC 
GATGATTTAA GGGTTCTGGG GAAGCAAGAC TTCAAGCATC TTCTAAAGTG GAGGATTCAA 
GTTAGAAAAG CTCTTTCACC AACTCAAAAG CCTGATTCTA CCACTACAGA ACTGATGGAC 
AATGAGCCTA AGGTGGTGGA TGAAGAAGAT AGAATACTCA ATGAAATGGA GGAACTGACT 
TATGTAATGG ACCGCAAGAA GAAACGTGCA AAGAAGCTTC TTGCAAAAAG AAGAGCCAAG 
GACAAGGCAC GAAAAGCAAC AGGGATGCAA ATGGATGCAA TAGATGATGG TTATGTAGAT 
CAAGAGTTGT TTGCACTTTC CTCTATTAAG GGTAAGAAGG ATCTGGTAGC TGTTGACAAC 
ACTGAGTATG AAGGTGATGA AGGAGAAGTA GAAGACAGTG AGAATGAAGA AACCCATGAA 
GGCCGCCCAG AGCATTCATC CAGTGACTTG GAAGATTCTG ATGAGGAACG TAAAAGATAC 
AATGAACAAA TGGAAGATTT GATGGATCAA GCTTACGAGA GGTTTGTGAT CAGAAAGGAA 
GGAAGTGCAA AACAGAGGAA GCGAATTAAA AAATCCTATG ATGCAAAGGC CCAACTTTTG 
GAGGGTGGTG AGGATGATGA TATTGTTCAA TCCAAGTATG ATTCAGATGA AGATCAGGGT 
GATCAAGAAG CAAATCCATT GATGGTGCCA CTTAATGATG AGGCAGAGCT CACCCAGGAG 
GAGATCATGA ATAAGTGGTT TAGTCAGGAT GTCTTTGCTG AAGCTGCAGA GGAAGGAGAC 
TTTAAGAAGG ATGAGAGTAA AGATGAAATG GATATAGATG AGCCTAAGGA GAAGATATCC 
ATTGCCAAAA AGGTAAAAGA AAATAAAACA GCAGCCCCAG CTGTAGCAAC TCACCCTCAG 
CCTCAACCAT CCAAGGCAGG GGATGATTTT GAAATAGTTC CTGCACCCGA TACTGATTCA 
AGTGATGATT CATCCTCTGA TGAATGGGAA GAAGATATTG AAGCCAAAGC TGAGATATTG 
GCTTATGCTA AGAAGATGAT GAGGAAAAAG CAACGGGAGC ACTTACTGGA TGATGCATAC 
AACAAGTACA TGTTTGATGA CGAAGGATTG CCCAAGTGGT TTCTAGATGA GGAGAGGAGA 
CATCGCCAAC CAATAAAGCC TATTACCAAA GAGGAAATTG CTGCGATGAA AGCACAGTTT 
AAAGAAATTG ATGCTCGACC TGCGAAGAAG GTGGCAGAGG CCAAAGCACG GAAGAAGCGT 
GTTGCAATGA GGAAACTTGA GAAGGTACGG AAGAAGGCCA ATGCCATATC AGATCAGACA 
GAGATATCTG ATCGTTCGAA GAGGAAGCAA ATTGAACAAC TATATAAAAG GGCTGTTCCT 
AAGAGGCCTA AAAAGGAATA TGTAGTGGCA AAGAAAGGTG TCCAGGTAAG GGCTGGCAAG 
GGAAAAGTCC TTGTTGATCG CAGAATGAAG AAGGATGCTA GGAAACATGG ATCGGGTAAG 
GGTGGAAAAG GAGGTTCTAA GACGAAGGGC AAGGCTCCAA AGGGCAAAGG AGGATCTTCA 
AAGGCCTCTG CCTCTGCAAA AAAAGGGAAA CAGAGAACTA AATGA