GIT1 cDNA ORF clone, Gavialis gangeticus(Gharial)

Gene
Clones
gRNAs

Summary

Gene Symbol	GIT1
Entrez Gene ID	109293160
Full Name	GIT ArfGAP 1
Gene Type	protein-coding
Organism	Gavialis gangeticus(Gharial)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019510965.1	XP_019366510.1	LOW QUALITY PROTEIN: ARF GTPase-activating protein GIT1

Pathways from BioSystems
Gene Ontology

The following GIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa73463	XM_019510965.1 Latest version!	Gavialis gangeticus GIT ArfGAP 1 (GIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GIT1 ( XM_019510965.1 ) cDNA ORF clone, Gavialis gangeticus(Gharial) -> XP_019366510.1 Gavialis gangeticus GIT ArfGAP 1 (GIT1), mRNA.

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CloneID	OGa73463
Clone ID Related Accession (Same CDS sequence)	XM_019510965.1
Accession Version	XM_019510965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-15
Organism	Gavialis gangeticus(Gharial)
Product	LOW QUALITY PROTEIN: ARF GTPase-activating protein GIT1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017729012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gavialis gangeticus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCCCAAA ACCGGGGCGG GGGGGCACCC AGAATGGGCA CGGGGCCGGG GGGATGCCGG 
GTGGCCCCCG GGGCCCTGAC ACCCGCCTGC CCCGCAGACC CTGGCTGGGC CTCCATCAAC 
CGGGGCGTGC TGATCTGCGA CGAGTGCTGC AGCGTGCACC GCAGCCTGGG CCGCCACATC 
TCCATCGTCA AGCACCTGCG CCACAGCCCC TGGCCCCCCA GCCTGCTGCA GGTGGGTCGC 
GCCCCCGAGC GCGGCCGGGG GGGCCACGGC CCCGCTGACC CCGTGCTCTC CCGCAGCCCC 
ACCAAGTCGG AGTTCATCCG GGCCAAGTAC CAGATGCTGG CCTTCGTGCA CAAGCTGCCC 
TGCCGCGACG ACGACGGCGT TACCGCCAAG GACCTCAGCA AGCAACTGCA CTCGAGCGTG 
CGGACAGGCA ACCTGGAAAC CTGCCTGCGG CTGCTCTCGC TGGGCGCTCA GGCCAACTTC 
TTTCACCCGG AGAAGGGCAC GACCCCGCTG CACGTAGCGG CCAAAGCCGG GCAGATCCTG 
CAAGCCGAGC TGCTCGTGGT GTACGGCGCT GACCCCGGAG CGCCCGATGT CAATGGCCGC 
ACCCCCATCG ACTATGCCAG GCAGGCGTCG CAGCACGAGC TGGCCGAGCG GCTGGTGGAG 
TGCCAGTACG AGCTGACCGA CCGCCTGGCC TTCTACCTCT GCGGCCGCAA GCCGGACCAC 
AAGAATGGGC ACTACATCAT CCCGCAGATG GCTGACAGGG TGCGCCCCAA GTGCGTGTCG 
CAGAGCCTGG ACCTGTCCGA GCTGGCGAAG GCTGCCAAGA AGAAGCTACA GGCACTCAGC 
AACCGCCTCT TCGAGGAGCT GGCCATGGAC GTGTACGACG AGGTGGACCG CCGGGAGAAT 
GACGCGGTGT GGCTGACGAC GCAGAACCAC AGCACACTGG TGACCGAGCG CAGCGCCGTG 
CCCTTCCTGC CCGTCAACCC CGAGTACTCG GCCACCCGCA ACCAGGGGCG GCAGAAGCTG 
GCCAGGTTCA ACGCCAGGGA ATTTGCCACC CTCATCATCG ACATCCTGGG CGAGGCCAAG 
CGGCGTCAGC AGGGCAAGAG CTTGCTCAGC CCCACAGACG CACTCGACTA CTCGCTGCGG 
AGCCAGAGCG ACGTGGACGA CCAGCACGAC TACGACAGCG TCGCCTCCGA CGAGGACACG 
GACCAGGAGC TGCTGCGCAA CGCCGCCCGC AACAACCGTG CCAGGAGCAT GGACTCCTCG 
GACCTGTCGG ACGGGCCCAT CACGCTGCAG GAGTACCTGG AGGTGAAGAA GGCGCTGGCG 
GCCTCCGAGG CCAAGGTGCA GCAGCTCATG AAGNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNCAC CGGCGGGACC GGCAGGCCTT CTCCATGTAC 
GAGCCGGGCT CTGCCCTCAA GCCCTTTGGG CAGCCTGTGG ATGAGCTCGT CACCCGGCTC 
CAGCCCTTCG GCTCCAGCGA GGCGGAGGAG GAGGCGATGT ACTCCATGCA CATCCCGGCC 
GGCGTGTACC GGATCCGTAA GGGGGTGTCG GCCTCCTCGG TGCCCTTCCC CCCCTCGTCC 
CCGCTGCTGT CCTGCCCGCC CGACAGCGCC CGCCACATGC CCAGCAAGCT GGACCGGCAC 
GGCAGTGGCA CGGACAGCGA CTACGACAAC ACGCAGGCGG GCGAGACCCT GCTCAGCATG 
GAGGGGAAGC GATTCATGGA GCTGGGCAAG GACGACGACT TCCACCCCGA GCTGGACCTG 
CTGGACGGGG AGCCGGACCC CGGGCTGCCC AGCACCGAGG ACGTCATCCT CAAGACGGAG 
CAGGTCACCA AGAACATCCA GGAGCTGCTG CGGGCTGCCC AGGAGTTCAA GCACGACAGC 
TTCGTGCCCT GCTCGGAGAA GATCCACTCC GCCGTCACCG AGATGGCCTC ACTCTTCCCC 
AAGAAGCCGG CGCTGGAGAC AGTGCGCAGC TCCCTGCGCC TGCTCAACGC CAGCGCCTAC 
CGGCTGCAGA GCGAGTGCCG CAAGACGGTG CCGCCCGAGC CCGGCGCGCC TGTGGACTAC 
CAGCTCCTGA CGCAGCAGGT CATCCAGTGT GCCTACGACA TCGCCAAGGC CGCCAAGCAG 
CTGGTCACCA TCACCACCCG CGAGAAGAAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019366510.1
CDS	119..2434
Translation	MPQNRGGGAPRMGTGPGGCRVAPGALTPACPADPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSPWPPSLLQVGRAPERGRGGHGPADPVLSRSPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYARQASQHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRVRPKCVSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILGEAKRRQQGKSLLSPTDALDYSLRSQSDVDDQHDYDSVASDEDTDQELLRNAARNNRARSMDSSDLSDGPITLQEYLEVKKALAASEAKVQQLMKnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullHRRDRQAFSMYEPGSALKPFGQPVDELVTRLQPFGSSEAEEEAMYSMHIPAGVYRIRKGVSASSVPFPPSSPLLSCPPDSARHMPSKLDRHGSGTDSDYDNTQAGETLLSMEGKRFMELGKDDDFHPELDLLDGEPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_019510965.1
Organism	Gavialis gangeticus(Gharial)
Definition	Gavialis gangeticus GIT ArfGAP 1 (GIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019510965.1

ORF Insert Sequence:

ATGCCCCAAA ACCGGGGCGG GGGGGCACCC AGAATGGGCA CGGGGCCGGG GGGATGCCGG 
GTGGCCCCCG GGGCCCTGAC ACCCGCCTGC CCCGCAGACC CTGGCTGGGC CTCCATCAAC 
CGGGGCGTGC TGATCTGCGA CGAGTGCTGC AGCGTGCACC GCAGCCTGGG CCGCCACATC 
TCCATCGTCA AGCACCTGCG CCACAGCCCC TGGCCCCCCA GCCTGCTGCA GGTGGGTCGC 
GCCCCCGAGC GCGGCCGGGG GGGCCACGGC CCCGCTGACC CCGTGCTCTC CCGCAGCCCC 
ACCAAGTCGG AGTTCATCCG GGCCAAGTAC CAGATGCTGG CCTTCGTGCA CAAGCTGCCC 
TGCCGCGACG ACGACGGCGT TACCGCCAAG GACCTCAGCA AGCAACTGCA CTCGAGCGTG 
CGGACAGGCA ACCTGGAAAC CTGCCTGCGG CTGCTCTCGC TGGGCGCTCA GGCCAACTTC 
TTTCACCCGG AGAAGGGCAC GACCCCGCTG CACGTAGCGG CCAAAGCCGG GCAGATCCTG 
CAAGCCGAGC TGCTCGTGGT GTACGGCGCT GACCCCGGAG CGCCCGATGT CAATGGCCGC 
ACCCCCATCG ACTATGCCAG GCAGGCGTCG CAGCACGAGC TGGCCGAGCG GCTGGTGGAG 
TGCCAGTACG AGCTGACCGA CCGCCTGGCC TTCTACCTCT GCGGCCGCAA GCCGGACCAC 
AAGAATGGGC ACTACATCAT CCCGCAGATG GCTGACAGGG TGCGCCCCAA GTGCGTGTCG 
CAGAGCCTGG ACCTGTCCGA GCTGGCGAAG GCTGCCAAGA AGAAGCTACA GGCACTCAGC 
AACCGCCTCT TCGAGGAGCT GGCCATGGAC GTGTACGACG AGGTGGACCG CCGGGAGAAT 
GACGCGGTGT GGCTGACGAC GCAGAACCAC AGCACACTGG TGACCGAGCG CAGCGCCGTG 
CCCTTCCTGC CCGTCAACCC CGAGTACTCG GCCACCCGCA ACCAGGGGCG GCAGAAGCTG 
GCCAGGTTCA ACGCCAGGGA ATTTGCCACC CTCATCATCG ACATCCTGGG CGAGGCCAAG 
CGGCGTCAGC AGGGCAAGAG CTTGCTCAGC CCCACAGACG CACTCGACTA CTCGCTGCGG 
AGCCAGAGCG ACGTGGACGA CCAGCACGAC TACGACAGCG TCGCCTCCGA CGAGGACACG 
GACCAGGAGC TGCTGCGCAA CGCCGCCCGC AACAACCGTG CCAGGAGCAT GGACTCCTCG 
GACCTGTCGG ACGGGCCCAT CACGCTGCAG GAGTACCTGG AGGTGAAGAA GGCGCTGGCG 
GCCTCCGAGG CCAAGGTGCA GCAGCTCATG AAGNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNCAC CGGCGGGACC GGCAGGCCTT CTCCATGTAC 
GAGCCGGGCT CTGCCCTCAA GCCCTTTGGG CAGCCTGTGG ATGAGCTCGT CACCCGGCTC 
CAGCCCTTCG GCTCCAGCGA GGCGGAGGAG GAGGCGATGT ACTCCATGCA CATCCCGGCC 
GGCGTGTACC GGATCCGTAA GGGGGTGTCG GCCTCCTCGG TGCCCTTCCC CCCCTCGTCC 
CCGCTGCTGT CCTGCCCGCC CGACAGCGCC CGCCACATGC CCAGCAAGCT GGACCGGCAC 
GGCAGTGGCA CGGACAGCGA CTACGACAAC ACGCAGGCGG GCGAGACCCT GCTCAGCATG 
GAGGGGAAGC GATTCATGGA GCTGGGCAAG GACGACGACT TCCACCCCGA GCTGGACCTG 
CTGGACGGGG AGCCGGACCC CGGGCTGCCC AGCACCGAGG ACGTCATCCT CAAGACGGAG 
CAGGTCACCA AGAACATCCA GGAGCTGCTG CGGGCTGCCC AGGAGTTCAA GCACGACAGC 
TTCGTGCCCT GCTCGGAGAA GATCCACTCC GCCGTCACCG AGATGGCCTC ACTCTTCCCC 
AAGAAGCCGG CGCTGGAGAC AGTGCGCAGC TCCCTGCGCC TGCTCAACGC CAGCGCCTAC 
CGGCTGCAGA GCGAGTGCCG CAAGACGGTG CCGCCCGAGC CCGGCGCGCC TGTGGACTAC 
CAGCTCCTGA CGCAGCAGGT CATCCAGTGT GCCTACGACA TCGCCAAGGC CGCCAAGCAG 
CTGGTCACCA TCACCACCCG CGAGAAGAAG CAGTGA