ZGRF1 cDNA ORF clone, Gallus gallus(chicken)

The following ZGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa35158 XM_015276342.2
Latest version!
Gallus gallus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35158 XM_015276340.2
Latest version!
Gallus gallus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OGa35158 XM_015276340.1 Gallus gallus chromosome 4 open reading frame, human C4orf21 (C4H4ORF21), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa35158 XM_015276342.1 Gallus gallus chromosome 4 open reading frame, human C4orf21 (C4H4ORF21), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa35158
    Clone ID Related Accession (Same CDS sequence) XM_015276340.1 , XM_015276342.1 , XM_015276340.2 , XM_015276342.2
    Accession Version XM_015276340.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein ZGRF1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131826.1
    CDS162..5498
    Translation

    Target ORF information:

    RefSeq Version XM_015276340.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus chromosome 4 open reading frame, human C4orf21 (C4H4ORF21), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35158
    Clone ID Related Accession (Same CDS sequence) XM_015276340.1 , XM_015276342.1 , XM_015276340.2 , XM_015276342.2
    Accession Version XM_015276342.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product protein ZGRF1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131828.1
    CDS143..5479
    Translation

    Target ORF information:

    RefSeq Version XM_015276342.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus chromosome 4 open reading frame, human C4orf21 (C4H4ORF21), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35158
    Clone ID Related Accession (Same CDS sequence) XM_015276340.1 , XM_015276342.1 , XM_015276340.2 , XM_015276342.2
    Accession Version XM_015276340.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein ZGRF1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131826.1
    CDS203..5539
    Translation

    Target ORF information:

    RefSeq Version XM_015276340.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276340.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35158
    Clone ID Related Accession (Same CDS sequence) XM_015276340.1 , XM_015276342.1 , XM_015276340.2 , XM_015276342.2
    Accession Version XM_015276342.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein ZGRF1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131828.1
    CDS161..5497
    Translation

    Target ORF information:

    RefSeq Version XM_015276342.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276342.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTTCTC AAGAATTTAC AGTGTTGTAC ACTCACCAAA AAATGAAAAA ATCAAAAACA 
    TGGCAAGATG GAATTCTGAA GATTAGACCT GGAGAAAATA AGGCAATCTT GCTGGATGAT
    AAAGGACAAT ACTTGGAGAG TATGTTTATA AAATCCCAGG TGAATCCTGG AGATAATTTA
    GAAAGTGAAC GATATCTGAT CACAGTTGAA GAAGCAAAAA TGAAGGAAAA ATCTTGCGAA
    GATCAACCAA GGAAAGCTGA AGTAGTAGAT GTGAATAGAC GTGGTGTAAA GCCTGGAGTT
    CTACCTTCAA GGCATCTGTC TGTTGGCTTA AAGAGGAAGT TTACAGGTTT CCAAGGGCCA
    CGTCAGGTTG AAAAGAAAGT ATCGACAGTA GAAGATGGAG AAGGAGTGGC AGCATTGCCT
    ATATCTGAGC AGAGTCGAGA TCCTTTTCCA TCCAGGTTTT ATATTACCTC TCCCTTATTT
    TCTACTGTTT ACAAGAAGAA TGCAGAAACA AATCTATGTA CAGACTTGCA TGAAGGTGGA
    GGTACAGATA ACAGAGATCA TGTGACTCCC TCCTCAGTGG TTTCATTTCC ATTTCTTCAT
    ACGCATAATG AAACTGAGAA GAAAAATTCT GATCAGTCCA TGGTAAAGCC AGAGTCTCCT
    GTATTTAGTG GGCACACCAA ATCTAGTAGT TGGACAGCTG GTCATGGGGC AGTGTCACAG
    AACATCAGGA GCGCTGCACA GATCATAGCT CTTTTGAAAT CTAAAACAAC ACAAGTCTGC
    AGAGAGCAAG CAACATCTGA AGTCACAGAA TGTCATAGAT CTCAAGCACT GGAAAACATA
    AATAGTTCAT ATAACCTAAA AAGCACAACC CTGCCTGCCT TTTCTGTCAA CTCTGACAAA
    AGAATTGCAC AAAATCTTCA GCACTTGCCT TTTAAAAAGG AAATGGTAAA TGATGAAAAG
    GAACAGAATA TTGAAATGCT TCTAAATTCA TCTGAACCTT GTGATAAAGA AATCACAGGG
    CAAAGACATG ACAAAAAGGC TAATAGTTTA AGCCGAGATT TACAAGATCC CTGCAGGACA
    AATAGTTCTG TACTAGCTGA ATTAACCATA AGCAGAGTGA GTGACAGCCA GTTTGTCTCT
    TCTTGTTCAG CAAGTCCAGT TGCCTTTGAA ACACCTGTCT CCTTAGACAG AGAGCATTTG
    GCAATTAATG GTCTTCAGGA TAATTCATCA ATCAAGTCTC AAAGTGACCT TCAGCTGAGA
    CAAAGCTCAG AAAGAGTGCC TAGTGAGATA GAGCTCTCTG AGGATGTAAC ACTGGCTGAA
    AATGGAATTG CAAAGCAGGG ATTAAGTACA CATGGCAGAG ACTGTAGTCC AGAAAAACAG
    GTGATGGAGG TTAATTTTGA CCTGTTGGGG GCTTTTGATT TTCATGAAGT GGACAATGGA
    GAGCACTGTG AAAGAAATAA GAAAGAGCTC ACTGAAGAAG ACATGCTTTC AAAAAGTGCA
    GATTGCTTAG AGGGAGAAGA TGGGGCACAA AATACTGCAC TGAGACTTCA TTCTTGCTTT
    GAAGTGATGA CACACTGTAA AAAGGAAGAA ATCAAGTGTT CAACATACGA TGTAGAAAAT
    GATGGCAATC GCTGTACTGT GAAAATGCCA TTACAGTTTT GTAACAGTGT TGCAGATACA
    GAGAGAATTA TGGAGGACTC TTCAAACCAG ACCCAGATTG AAGTGGAACT TTTGGATGAT
    AGAAACAACT TAAAAGAGAT TAATGAAAGC CTAAAAACTG AGCTGTGCGT TCCACTGAAA
    GTACTGGGCC AAAGAGACCC TAGGGCATCT GACTGCCAAT GGAAGTCTCC AGATTCAAGA
    AGTCTTCATG AGGACGATAT GAATTTTGTC AGAGAGGTGA ATTTTCAGAA GAAGTTTAAC
    GTTAAAGACT CAGAAGTAGA TCACTCCTCA CTGGCATTGA GTGCGTGTTC TGAAGTTCTG
    CCGTGGATAG TGTCAAGCTC ACCATCACAT TCTGATTTGG GGGAAAAGCA GCAGACTTCA
    TGGGAACCTG ACAAGATTAT TTCATCAGTG CAATTGAATT CCTCAGTTTC TCCAGCTTCA
    GGAAAAAAGG GAAGTGTTCA AGACATACAG GAACCTCTGA TTCACAGGAG CCTACCAAGT
    GATTCAGAAC TTTCAGAAAA CATTTTCACA TGGGAGCAAG ACAGCTATCC AGAGGAATGC
    AACTCCACAG TTGAAACACA GACTCCACTT GTCACTGTTG CTGAGAAGAT TCCCAACACC
    GAATTTTTCC TAACTGACAC TGAGGAGGTC CAGCAATCTG ATGGCTTTTC AGTAGTCAGT
    TTGAGCAACA AGCCTGCGGT GTTTCCTATG AGTGCTTCTG GCCCTGAGGA CAGGAATTAT
    GAAACCTCTG TGTTTGAGGA GTGTACGGAA GACAGACAAA GGGAATCTGC ACACCCAGTG
    TTCTCAAATG GGACTTCACA ATATAGACAA AGCAAGTGGC TAAAATATCT AAACAGCGCT
    CATTGTGACT CAATAACTGA AAACAGCAGT GATATGGAAG TGACTGATGA CACCTATGCT
    GAGAACGTCC TTGGAATGCT GTCGGGTGAC ACAGGAGAGA GCCAGAGCAG CACTGTGAAT
    AACAACACTC CCGGCTCTGT ACCTCTGCTG AGAGCAAAGA GCGTGCTTAG TGAATGCTGT
    GCAGATAGCA GCAACCAAGA TTTGATTTCA GAAAGGAAGT TACTTTCTCT TCACTTAAAT
    CAGACTCCTT TAACTGAACC AGCACAAAAG GTGTTGAGTC ATCTGACCTG CCGCACTGTG
    ACAGGAGATG TCCAGGACAT AACAGTTTCT GAGTTGTCTT TTCCTAATGC AGATAAAGTA
    AAACATGCTA GTCTTCCTAA AAGAAAGATT GCCATACCAA CTGAGTTTCA GTCTCACGTT
    CACTACAGAC AGGTTTTTAA AGCTGCTTTG ACAGAACATT TAAACATAAT GCTTTTCGAG
    TTGTCACAAA GATTACACAG CGCTCTTTTG AAAGTGGATA TATCATCTTA CACTTCACTG
    GAAGATGAGC AAAGCGAGAG CAAAGAAAGC TGCGTTCCAG TCTGTGATCA CATGCGTCCT
    GCTAAGGTTC TTGTGGTTAA AAAAGAAGGT CAAAACAAGG GCCGTCTGTT CTATGCCTGT
    GATGCCCCAA GAGCTGAGCG GTGCTCATTT TTCAAGTGGA TAGAAGAAGT GAATCCTTCA
    CAAATAAAAA AATTCAGACC AAGTGTAGTG CTTCATGATT TAAGAAGTAT TGGGGCGTAC
    CTCCGAAATG AAAAGATTTT TGTCTTTGAT GGATGCCAGC TTTTTGTGAG GAAAGGCTAT
    GAAATTGGAA CACGGTGTAG CAAACTCAAG AAACTTATGA ATGTGCATGC TGAATCTGAT
    GGGGATTCCA AAAGTAAATT GTACCTCAAG CTAAGCAGAA AGGAGCATTA TTCCCTCTTT
    AGCAAAGATG ATATTTGGGT TGTTTCAAAG ACTCTGAATT TTGATTCCCT TGATACCTTC
    ATTGCAAGTA GTGCTTTCTT TGGACCAACC CATAACAACG AAGTAGAACT ACTACCACTG
    AAAGGCTATT ACCCCTCAAA CTGGCACTCA CATACATCAG TTCATGCCTT GCTAGTTTGT
    AATGCCAGTG GTGAGCTTAT ATCCTTAAGA AATATGGAAG AATACTTCAA CCCATCTACT
    TTACCACTAA TACCGTATCT ACTAAAAATG AATTCTGATT CTGAAAAATC AACTAGAAGA
    ATCAACAAAA GAAAATTTAT TCCACCTGCC ATGAGCCTGG TAGGCAAAAA GACCTATGGA
    TCTGTCAGCA CTGAAGTGGC AATGGAACTA GCTGAAAAGA TGATCCTGAC GTTCTCATTG
    AATCCAGATC AAGCTATGTC TCTGATTCAC ATAGCTCAGA TGATGACCTC ACATGAAAAT
    ATGAAACCAG CGGAAGAACA TCAGATTTTC CCTATCACAA TCATACATGG TGTTTTTGGA
    GCTGGAAAGA GCTTTTTGCT GTCTGTTGTG ATCTTGTTCC TGGTCCAGCT CTTTGAAAGC
    AGTGAAGCTA AGGAGGGTGC AGAGCTAGCT CCATGGAAAC TTCTGATTGC TTCTTCTACT
    AATGTTGCTG TTGACAGGAT ATTGCTGTGT CTACTTGATC TTGGATTTGA GGATTTTATC
    AGAGTGGGAA GTATTAGGAA AATCACCAAA GCAATTCTTC CCTATAGCTT ACATGCTGGC
    TCAGGAAATG AAAATGAACA GTTAAAAGAA CTTCTTGCTC TCATGAAAGA AGATTTAGCT
    CCAGCTGAAA AAATGTATGT AAGGAAGAGT ATTGAGCGGC ATAAACTGGG GACCAATAAA
    ACTGCATTGC AACAGGTAAA AGTGGTTGGA GTGACCTGCG CTGCCTGCCC GTTCCCTTGC
    TTGGATACTC TTAAGTTTCC TGTAGTGGTG CTGGATGAGT GCAGTCAGAT GACTGAACCT
    ACTTCTCTCC TTCCTATTGC AAGGTTTCAG TGTGAAAAGC TAGTCCTCGT TGGAGATCCT
    AAGCAACTGC CACCAACTAT TCAAGGGTCT GAGAGCATTC ATGAAAAAGG ACTGGAGCAG
    ACTCTCTTTG ATCGGCTTTG CTTAATGGGA CATAAAACAA TACTGCTACG GACACAATAC
    CGATGCCACC CCGCTATTAG TGCCATAGCC AACGAGCTGT TCTATGAAGG AAACCTGATA
    GATGGTGTTT CTGAGAAGGA AAGAAGTCCT TTACTGGATT GGCTTCCAAC ACTTTGTTTT
    TATAGTGTTA ACGGGCTGGA GCAAATTGAA AGAGACAACA GTTTTTACAA CATGGCAGAA
    GTTCATTTTA CAGTCAAGTT TATCCAGGCC CTGATTGCAA GTGGAATAGA TGGATCTGCA
    GTGGGCGTGA TTACTTTTTA TAAGTCACAG ATGTACAAGT TACAGAACTT GCTTAGGAGC
    ATACATTCTG AGGCTTTTCC AGTGAAGGCT GTCCAGGTGT CCACTGTAGA TGCATTCCAG
    GGAGCCGAGA AGGAGATCGT TGTTCTGTCG TGTGTAAGAA CAAGGCAAAC TGGATTCACA
    GACTCAGAAA AGAGAATGAA TGTTGCACTA ACAAGGGCAA AGAGGCATCT GCTGATTGTT
    GGAAATCTGG CCTGCTTAAG TAAGAACAGA CTCTATGAAA GAGTAATTAA TCACTGCAAA
    GGACGAGAAA ATGGATTGCA ACATGGAAGC CAATGTGAGC AGCAGTTAAA CAACGTTCTT
    AAGTACTACT TGGAGGAAAA GAAGGAAGAG GAACAGAGTA AGAAAAAGGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.