NOS1 cDNA ORF clone, Gallus gallus(chicken)

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa144742 XM_015294875.2
Latest version!
Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa15889 XM_004934480.3
Latest version!
Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa15889 XM_015294874.2
Latest version!
Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa15889 XM_015294873.2
Latest version!
Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa43086 XM_004934480.2
Latest version!
Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa43086 XM_015294874.1
Latest version!
Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa43086 XM_015294873.1
Latest version!
Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa43087 XM_015294875.1
Latest version!
Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa144742
    Clone ID Related Accession (Same CDS sequence) XM_015294875.2
    Accession Version XM_015294875.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150361.2
    CDS700..5007
    Translation

    Target ORF information:

    RefSeq Version XM_015294875.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294875.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa15889
    Clone ID Related Accession (Same CDS sequence) XM_015294873.2 , XM_004934480.3 , XM_015294874.2
    Accession Version XM_015294873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150359.2
    CDS554..4861
    Translation

    Target ORF information:

    RefSeq Version XM_015294873.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa15889
    Clone ID Related Accession (Same CDS sequence) XM_015294873.2 , XM_004934480.3 , XM_015294874.2
    Accession Version XM_004934480.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004934480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004934537.3
    CDS699..5006
    Translation

    Target ORF information:

    RefSeq Version XM_004934480.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004934480.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa15889
    Clone ID Related Accession (Same CDS sequence) XM_015294873.2 , XM_004934480.3 , XM_015294874.2
    Accession Version XM_015294874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150360.2
    CDS337..4644
    Translation

    Target ORF information:

    RefSeq Version XM_015294874.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG
    ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43086
    Clone ID Related Accession (Same CDS sequence) XM_004934480.2 , XM_015294873.1 , XM_015294874.1
    Accession Version XM_004934480.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004934480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004934537.2
    CDS686..4993
    Translation

    Target ORF information:

    RefSeq Version XM_004934480.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004934480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43086
    Clone ID Related Accession (Same CDS sequence) XM_004934480.2 , XM_015294873.1 , XM_015294874.1
    Accession Version XM_015294873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150359.1
    CDS560..4867
    Translation

    Target ORF information:

    RefSeq Version XM_015294873.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43086
    Clone ID Related Accession (Same CDS sequence) XM_004934480.2 , XM_015294873.1 , XM_015294874.1
    Accession Version XM_015294874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150360.1
    CDS338..4645
    Translation

    Target ORF information:

    RefSeq Version XM_015294874.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43087
    Clone ID Related Accession (Same CDS sequence) XM_015294875.1
    Accession Version XM_015294875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150361.1
    CDS687..4994
    Translation

    Target ORF information:

    RefSeq Version XM_015294875.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
    TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC
    GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT
    GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG
    CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT
    GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC
    CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT
    CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC
    GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC
    AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG
    CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT
    GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG
    GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT
    GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA
    GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT
    TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC
    ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT
    GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG
    CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC
    AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC
    CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC
    TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA
    GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC
    CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC
    TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG
    GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC
    GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT
    GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT
    AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT
    GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG
    GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG
    AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC
    ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC
    ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT
    GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG
    ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC
    ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG
    GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA
    GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT
    GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG
    CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA
    CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC
    CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA
    CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA
    GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC
    TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG
    GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC
    TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC
    AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG
    GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA
    GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC
    CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT
    GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT
    CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC
    GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT
    AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG
    TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG
    TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC
    TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC
    TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG
    GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG
    CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC
    CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC
    CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC
    TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG
    GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG
    GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG
    GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG
    GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG
    GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT
    TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA
    TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.