SPEF2 cDNA ORF clone, Gallus gallus(chicken)

The following SPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa153023 XM_025144817.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa153019 XM_025144812.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa153020 XM_025144813.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa153021 XM_025144815.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa153021 XM_025144814.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa153022 XM_025144816.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa153018 XM_025144811.1
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa47840 XM_015277513.2
Latest version!
Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
View Old Accession Versions >>
OGa47840 XM_015277513.1 Gallus gallus sperm flagellar 2 (SPEF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa153023
    Clone ID Related Accession (Same CDS sequence) XM_025144817.1
    Accession Version XM_025144817.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGGAATT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000585.1
    CDS35..4267
    Translation

    Target ORF information:

    RefSeq Version XM_025144817.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144817.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGGAATT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153019
    Clone ID Related Accession (Same CDS sequence) XM_025144812.1
    Accession Version XM_025144812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5886bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGATAGGAA AGCGGGGGGC TGCAACAAAA CTTTTATATG AATTGTACAT TGCTTTAGAA 
    AAAAAAAGAA AGACAAAGCT CACTGGAGTA GCTATGGAAG CAATGAGACC TGCAGCACCT
    GCAAGGTTCA AGTCTATTGG AAGTGTTTCA TTTCGAGAGC GTTTGAAATC TTTAACATCA
    CATCAGGCTG ATTTGCAGCT ATGGCAGGTT TCAGATCATT TTGAAATGAA ATCTAAAGCA
    ATAGAAGATA AAATAGCTCG TATACATATT TCAGAACAAC AGAAATCTCT AAAGCCTCAA
    GAGGAACAAA GAGCCCAAGA CTCTGAAAAG CATCGCATAG GAAGAAGACA AAATGAAATA
    ATGGCCCGGA TTCAAACAGC CGTTATACAA GTTCCAAAGC CATCACTAAG CCACACCTTA
    AAAAGTAACG AAGCAAAAAA AATGCTAAAG AAGAAAAGAG AAGCGGAGGA CACTTACAAG
    GAGATTAACA AGTTTGAGAA GTCCCTGGTA GGAAATGCTT TTGAAGTACA CAGCCAAGTC
    ACTGATAGTG AAATACAAGA ATCATTGCAA ACACAGAAGT TGCAAGAAAC AACCCTAGAA
    ACTACAGCAC AGGCAGCAAC TGAGCTATTA CCTACTTATT CTGATGATGA CTATGTAAGA
    AAAATTCAAA AATATTTGGA GGAAGATATG TTTGCTAGAG AACAACGAGA AAAGAGACGT
    CGTAAAATGT TAATGGAACA GTTGATAGCA CATGAAGCAG AAGAGGAGAT TTACCAGGAG
    GAGCAACTTG TTTACAGGCT AATGAGACAA TCTCAGCAGG AGCGGAGGAT TGCTGTTCAA
    CTCATGCATG TTCGACATGA GAAAGAGGTG TTAAAACAAA ATAGAATTTT CAGGGAAAAG
    CAATATGAAG AAAGACGACT GAAAGAGTTC CAGGAAGCTC TTGACAGAGA GGCGGCTCTT
    GCAAAACAAG AAAAAATTGA TTATGAAGAA CAAAGTGTCA AGGAAAGAGA GCGTCATGAG
    AAAATTGCCA TTGAAAGAGC TCAAGCTCGT TATAAAAAGC ATTATTCTGC ATGTTGGGAA
    GTGATTGATC AGATTGTTGA TTTGTCAACA AAAATAGGAG AGTATCGAGT ATTGACAAAC
    AACAAAGTTC CGTTAAAACT GATCTGTGAT TGGAAGGAAT TATTTTTTAA TGGAAACTCA
    ATATATGAAA AAGCCTCAAT TAAAACTTTG CCAAGTGAAC CAAGCCCAGA ACAACTTCTG
    GAGTTGAATA AAATGGATCT ATTAGATGAA AAAGATTATG ATGAATACAA GAATATGACA
    GGGGAATGGT GCCCAACTGA AGAGAACAGA GAAAATAAAT CTCTTAATAA TAACATATTG
    GGTCATGTTC TTCATAGACT GATGGAGATT TTCTATCCTT CAAAACCCAG ATGTACACTA
    CCTGTACTTC CTTCATTTCC CATCAAGGGA TGTATTCTGG GAAAAGTTTT TAGTGGAAAA
    ACTACCTGTG TAAAGTTTCT TGAAAAAGTT TGCAATATTC AGGTACTGTC TGTTGATACT
    CTGGTTCAGG AGGCCATCCA AGCTTTTCTT AATAATGAAA TGAAAACTGA ACACAGCTTG
    ATTTCACAGG AAGCAGAGAG TTCTGCAAAA CAATACAAGG TCCAGAGGAA CTTCTCAGAA
    ACATCAATGA AATATCTAAC AGACACATTT ACTAATGGAA CCAAAGGTAA TCATCCTTTC
    AAAAAGCACC CATCTGATCA GGAAACTGTA TCAGACCACA AAGAGAATGA CATATCCAAG
    CTATCTGTAC GTGCTCAACT TGGTGCTGCA TCTCAGAAAT TGTTGAAGAA AGGAAAAAGT
    ATTCCTGATG AATTACTAGT TGACATCCTA TTGGAAGCCA TTAAGCAAAC ACCACCAGAG
    AAAGGATGGA TTCTGGATGG ATTTCCAACG ACAATTAATC AGGCAAGGCT ATTTGAAAAA
    GCCTGTACAG GGATTGATCC AGATGAAACA GAAGCAAAAT ATGTGAACTG TGGAAAGCTC
    TCACTTGTAA CAAATCCTAG AACACCTAGC AAGCCTCCTG TCACCTTACC AGCATTTGAT
    GTTTCTATAT TATTGGATAT TTCAGACACC ACAGTACTGA AACGTGTGGC TAGCTTGATG
    TCAGATAAAT CAAAGCCCTC TGAAATTGAA CAGGAGGACA GCAGTCAAAA TTCAGATGTT
    AAAATCGTAG AAGAGAAGAC TGACTTAGCA AGGGACCAGG TGCTGCATAG ACTTTCAGGC
    TTCCTAGACA CATGGCCAAA ATTAGAGAAC TGGTTTTCAG ACCATCAGAA TACTCTAGTG
    AAGGTCAATG CAGAGATGGA AGAAAGTACT GTGTGCAAAA CTGTAAAGGA AATTCTGATT
    GAAGAAATTT ACAAGAAGCA GAATAGAAGG TATGTACAAG AAAAATCACC AGAAGAAAAG
    GCTTTGCCAG TTACACCTCA AGATCTGCTT TCCCCTGTTT CTGTGACACC ACTGATTAAG
    CCAGGAACAG ATGAATGGAT TTATGTAGAT GAACCACTTC CAAAGGAGAT ACCTGATTTT
    TTAGTACCTT ATTGGGAAAT GGTAGAAAAT GTGTATGTGA ATACCATCAA AACTGTACTT
    AGATGTCTAC GGGACGAACA GTACTCTGTG ATATATTATT TAGCAGATAT TAGAAAAAAT
    TTCCAAGATT ATTTAAAGCA TCCAGATCAT AAGCAGGAGT TTGTATCTCA GTGGCAGTCA
    GATTTTAATT CAATTCCTGA TGACCTGCGG GAAGATGAGG AAACAAAAGC AGAACTACAC
    CAAAGAGTAA CTGACTTAAG AGATCTTCTC TGGGATATCT GTGATAAAAG AAGGGAAGAA
    GCAGAGCAAG AACGTACTGA TATCATGAAT GATGGCTGGT TGCGAGATCA TAAAGGGATT
    GCAATAAACC ATTTTTTTTC ACTTATGCAG GTTGAAGTGG ATCGTTTCCA AGATACAAAG
    AGACTTCTTC ATGACTATTA TAGAGCAATG GAAGGAAAAA TCCCAACAGA GGACAGACAA
    AGTTTTACTA GACTTCCATT GCTAGATATT ATTGATAAAG AGCAGCAAGA AGATCAGAAT
    GAATCAAGAA GGATTCCTCT AGTTTCTTAT AAACTGCCTT CACCAGAAAC AAATATTACT
    AAGTCAAAAA GCAAGGGAAA TCTACTGAAG AGTGTTAAGG ATGAGAATTC TTCTGAAAAT
    GTAGTGGTCG CTCTTGGGAA AGATGAAAAT GTTATTCGTG ATACGTGGCA AACAGCAGTA
    ACAGCAGTAT CAGATATGGT AAGAGCAGAA ATACAGTCAA AAGAGGTGAA GGAAGAAGGA
    GAAAAGCAGA CAGAAGTGAA AGAAGATACA AAGTCTTCTC AAACAATATC AAGCAAATCT
    ACTGGGAAGG ATGCCAAAGA CACCAAAAAA TCTCCCAAAT CACCTACTAA AACGAAAGGA
    CCTCGCTCCA CTGCATCTGC AGCTGAAGAC AGTCCAGTTC CGTTAACCGC TGAAGAACTG
    AAGAAACAAA AACTGATACT TAAAATACAG CAAGAATATT TTGCTGCCCT GAAACATGAA
    GAGAAAGCTA CAAAAACTCG ACTCAAACTT ATAAAAGCAA AAGCTTTAGC ATATGTTGAA
    GAGTTGACAA TGAAAGCAGA AGAAGCTTAT AAAGATATGG AAAAGTGGCT TGGAGAAAGG
    TTCCTAGCAG AAATGTCCAG TGTTGAAAAG CTGATTGAGG TAGGACGACA CCATATTGAA
    AGCTCTAGCA AAATCCAGTA TGAAATGAAT TTAGAAGAAA CAGATTTCTT CATCAGCAGT
    GATGTAAAAG TGATTCCTGA TCCTGTTCCT CCACCACAAG TTCCACTAAT AGAGACTTCT
    GAAAGTAGTA CTCTAACTAT TTCGCAACTT AACACATTTC ATAAACATTT TCTACAAGTG
    GCACCTAAAG CCATAAGAGC ACTGAAGCAA TTTAAAGGCA GGAGTAATTC TTCTAGCAAG
    AGAGAAGTTG ATGACAGAGG GAAGGAAAAG AAAGATTTGG AACATCCAAG AATAAGGAAG
    AATCGCCGGT TTCCAAAAAT ATGGCTAACA AAATACTCCT GGTTAAAATA TGATGACGAA
    AGGGGAATAA TGTTCTGTGC ATTATGTCGC AAGCATAATG TGGACCTGGG GGAAAGCATT
    CATAATTTTT GTTCTGGTAC TGATGACTTC AAACTGGAAT TTATAAATAC ACACCAGAAC
    AGTGAAGCTC ATGCCTGGGC TACCTGCATG GAGGCTGCCA GTACTGCTTC ACCAAATACA
    GTTTCTGCTG AGCAGACATT GAAGAGTATG AACTCCATAA CAATAGGAAG AGTAGAAAGT
    ACTTTTAGAA CATGCCATGC AATTGCTAAA TCTGTTCAGC CCTTTACAGA CCTGGACTGG
    ATTTATAAAT CAGATGATGT GAAAGGAACA GATATTGGTT CTCTATTCAG AAATTATAAG
    TCAGCAAGAA TGTTTAAACA TTTTATGGCT GAAGCAGAAA GAAGAGCTCT AAAAGAGGAA
    CTGGAGAATT GTAAATTCTT TTCTCTTATT AGTGATGAAT TCACAGATAG TATATCCAAA
    TCAGCTGCAG TTGTCTATGT GCACTTTGCA AATGAAGGAA AAGTTCACTG CCAGATTGTT
    GGGGTTCAAC CTGTTCAAAA AAATGATGCT TCAACTATAA AAAGTGCAAT TGAGGAAACA
    TTTCAAATAA ATCTTCAGCT TAGCTTGGCA AGCCAAGACT GGTCAAGAAA ATTAGTTGGA
    TTTGGAAGCG AGGAGACAGA TGTAATGACT GGTCAAAATA ATGGGGTAGC TAATCTCATG
    AGGGAAATTC AACCTTGTGT GCAGTCTGTG CATTGTTTTG CTCACCGCCT AAGGCTGGCA
    TACAAGGGAG CACTGGAAAA CATTCAGCTA TACAGCTTAA CCAATGGCTT GAGAAGTATG
    TACTACTTTT ATCATAACTC AACTCTAAAT AAGAAGAATC TCAAAGCTAT ATATGAAGCC
    ATCAAGTTAC ATCCAGCTAT TCCATCTTGC ACTGGGGGCT CCCAGTGGTT TTCTCGCCTA
    CAGACAGCTC TACAAATTCT CCTTAAAGGT CATCCTGCAT TTGTTCTACA TCTGGATAAG
    ACTAAAAGAG ATTCAAGGTC CTCAAATAGG CAGAAAGTCA AAGGACTATT GTATCTCCTT
    CTGAAAATGG AGATAGTCAA GTTTTCCCAT TTCTTGTTGG ATGTCATCAA TGTCCTCAGC
    ATTCTGTTAC ATATTACTGT GGATCACAAT TCCTCCATTG CAGATATATT TGCAACTATG
    CAGTCAACTG TGGAAACACT CCAAATGTAT CAAGCAAGAG CTGGTCCAAT GGAACGCTTA
    ATGAAGGCCA TTCAACATTT TCATGGTCAC CAGCTTGTTG GAAATGGAAA TATTTCAGCA
    GTACGAACCA AAGTACCAAG TAATGTGGTG AAGAGGTTAA GTGATTGTTT CTGTGATGCT
    AATCAAGGTG TCTTGAAAGC AACTCTTATT GGAAGTTTTA ATCTATGGCC TGACAAAATG
    AAACAAGTTA TAGTATTTGG TGAAAAGGAA GTATCTGTCT TGAGTAAACA TTATGAAGCC
    ATACTAGAAG CTGCTAGTGT GAAGATCAGT GAAGTTGAAA TTGAATGGAG CATGTTGAAA
    TTAGAGCTAT ATAACAGATT TCAGAACATC CAGACCTTGA CATGGGATTC GGTGAATGCA
    CATTATTCAA AGAAGTATCC CAGTATCCTG GCATTGGTAG ATCTGATCCT AACTCTGCCA
    GCAAGCTCAG CTGAAATAGA CCAAGGCTCT GGAAAAGAAA GTCATCATGA TGCATCTGCA
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000580.1
    CDS338..6223
    Translation

    Target ORF information:

    RefSeq Version XM_025144812.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGATAGGAA AGCGGGGGGC TGCAACAAAA CTTTTATATG AATTGTACAT TGCTTTAGAA 
    AAAAAAAGAA AGACAAAGCT CACTGGAGTA GCTATGGAAG CAATGAGACC TGCAGCACCT
    GCAAGGTTCA AGTCTATTGG AAGTGTTTCA TTTCGAGAGC GTTTGAAATC TTTAACATCA
    CATCAGGCTG ATTTGCAGCT ATGGCAGGTT TCAGATCATT TTGAAATGAA ATCTAAAGCA
    ATAGAAGATA AAATAGCTCG TATACATATT TCAGAACAAC AGAAATCTCT AAAGCCTCAA
    GAGGAACAAA GAGCCCAAGA CTCTGAAAAG CATCGCATAG GAAGAAGACA AAATGAAATA
    ATGGCCCGGA TTCAAACAGC CGTTATACAA GTTCCAAAGC CATCACTAAG CCACACCTTA
    AAAAGTAACG AAGCAAAAAA AATGCTAAAG AAGAAAAGAG AAGCGGAGGA CACTTACAAG
    GAGATTAACA AGTTTGAGAA GTCCCTGGTA GGAAATGCTT TTGAAGTACA CAGCCAAGTC
    ACTGATAGTG AAATACAAGA ATCATTGCAA ACACAGAAGT TGCAAGAAAC AACCCTAGAA
    ACTACAGCAC AGGCAGCAAC TGAGCTATTA CCTACTTATT CTGATGATGA CTATGTAAGA
    AAAATTCAAA AATATTTGGA GGAAGATATG TTTGCTAGAG AACAACGAGA AAAGAGACGT
    CGTAAAATGT TAATGGAACA GTTGATAGCA CATGAAGCAG AAGAGGAGAT TTACCAGGAG
    GAGCAACTTG TTTACAGGCT AATGAGACAA TCTCAGCAGG AGCGGAGGAT TGCTGTTCAA
    CTCATGCATG TTCGACATGA GAAAGAGGTG TTAAAACAAA ATAGAATTTT CAGGGAAAAG
    CAATATGAAG AAAGACGACT GAAAGAGTTC CAGGAAGCTC TTGACAGAGA GGCGGCTCTT
    GCAAAACAAG AAAAAATTGA TTATGAAGAA CAAAGTGTCA AGGAAAGAGA GCGTCATGAG
    AAAATTGCCA TTGAAAGAGC TCAAGCTCGT TATAAAAAGC ATTATTCTGC ATGTTGGGAA
    GTGATTGATC AGATTGTTGA TTTGTCAACA AAAATAGGAG AGTATCGAGT ATTGACAAAC
    AACAAAGTTC CGTTAAAACT GATCTGTGAT TGGAAGGAAT TATTTTTTAA TGGAAACTCA
    ATATATGAAA AAGCCTCAAT TAAAACTTTG CCAAGTGAAC CAAGCCCAGA ACAACTTCTG
    GAGTTGAATA AAATGGATCT ATTAGATGAA AAAGATTATG ATGAATACAA GAATATGACA
    GGGGAATGGT GCCCAACTGA AGAGAACAGA GAAAATAAAT CTCTTAATAA TAACATATTG
    GGTCATGTTC TTCATAGACT GATGGAGATT TTCTATCCTT CAAAACCCAG ATGTACACTA
    CCTGTACTTC CTTCATTTCC CATCAAGGGA TGTATTCTGG GAAAAGTTTT TAGTGGAAAA
    ACTACCTGTG TAAAGTTTCT TGAAAAAGTT TGCAATATTC AGGTACTGTC TGTTGATACT
    CTGGTTCAGG AGGCCATCCA AGCTTTTCTT AATAATGAAA TGAAAACTGA ACACAGCTTG
    ATTTCACAGG AAGCAGAGAG TTCTGCAAAA CAATACAAGG TCCAGAGGAA CTTCTCAGAA
    ACATCAATGA AATATCTAAC AGACACATTT ACTAATGGAA CCAAAGGTAA TCATCCTTTC
    AAAAAGCACC CATCTGATCA GGAAACTGTA TCAGACCACA AAGAGAATGA CATATCCAAG
    CTATCTGTAC GTGCTCAACT TGGTGCTGCA TCTCAGAAAT TGTTGAAGAA AGGAAAAAGT
    ATTCCTGATG AATTACTAGT TGACATCCTA TTGGAAGCCA TTAAGCAAAC ACCACCAGAG
    AAAGGATGGA TTCTGGATGG ATTTCCAACG ACAATTAATC AGGCAAGGCT ATTTGAAAAA
    GCCTGTACAG GGATTGATCC AGATGAAACA GAAGCAAAAT ATGTGAACTG TGGAAAGCTC
    TCACTTGTAA CAAATCCTAG AACACCTAGC AAGCCTCCTG TCACCTTACC AGCATTTGAT
    GTTTCTATAT TATTGGATAT TTCAGACACC ACAGTACTGA AACGTGTGGC TAGCTTGATG
    TCAGATAAAT CAAAGCCCTC TGAAATTGAA CAGGAGGACA GCAGTCAAAA TTCAGATGTT
    AAAATCGTAG AAGAGAAGAC TGACTTAGCA AGGGACCAGG TGCTGCATAG ACTTTCAGGC
    TTCCTAGACA CATGGCCAAA ATTAGAGAAC TGGTTTTCAG ACCATCAGAA TACTCTAGTG
    AAGGTCAATG CAGAGATGGA AGAAAGTACT GTGTGCAAAA CTGTAAAGGA AATTCTGATT
    GAAGAAATTT ACAAGAAGCA GAATAGAAGG TATGTACAAG AAAAATCACC AGAAGAAAAG
    GCTTTGCCAG TTACACCTCA AGATCTGCTT TCCCCTGTTT CTGTGACACC ACTGATTAAG
    CCAGGAACAG ATGAATGGAT TTATGTAGAT GAACCACTTC CAAAGGAGAT ACCTGATTTT
    TTAGTACCTT ATTGGGAAAT GGTAGAAAAT GTGTATGTGA ATACCATCAA AACTGTACTT
    AGATGTCTAC GGGACGAACA GTACTCTGTG ATATATTATT TAGCAGATAT TAGAAAAAAT
    TTCCAAGATT ATTTAAAGCA TCCAGATCAT AAGCAGGAGT TTGTATCTCA GTGGCAGTCA
    GATTTTAATT CAATTCCTGA TGACCTGCGG GAAGATGAGG AAACAAAAGC AGAACTACAC
    CAAAGAGTAA CTGACTTAAG AGATCTTCTC TGGGATATCT GTGATAAAAG AAGGGAAGAA
    GCAGAGCAAG AACGTACTGA TATCATGAAT GATGGCTGGT TGCGAGATCA TAAAGGGATT
    GCAATAAACC ATTTTTTTTC ACTTATGCAG GTTGAAGTGG ATCGTTTCCA AGATACAAAG
    AGACTTCTTC ATGACTATTA TAGAGCAATG GAAGGAAAAA TCCCAACAGA GGACAGACAA
    AGTTTTACTA GACTTCCATT GCTAGATATT ATTGATAAAG AGCAGCAAGA AGATCAGAAT
    GAATCAAGAA GGATTCCTCT AGTTTCTTAT AAACTGCCTT CACCAGAAAC AAATATTACT
    AAGTCAAAAA GCAAGGGAAA TCTACTGAAG AGTGTTAAGG ATGAGAATTC TTCTGAAAAT
    GTAGTGGTCG CTCTTGGGAA AGATGAAAAT GTTATTCGTG ATACGTGGCA AACAGCAGTA
    ACAGCAGTAT CAGATATGGT AAGAGCAGAA ATACAGTCAA AAGAGGTGAA GGAAGAAGGA
    GAAAAGCAGA CAGAAGTGAA AGAAGATACA AAGTCTTCTC AAACAATATC AAGCAAATCT
    ACTGGGAAGG ATGCCAAAGA CACCAAAAAA TCTCCCAAAT CACCTACTAA AACGAAAGGA
    CCTCGCTCCA CTGCATCTGC AGCTGAAGAC AGTCCAGTTC CGTTAACCGC TGAAGAACTG
    AAGAAACAAA AACTGATACT TAAAATACAG CAAGAATATT TTGCTGCCCT GAAACATGAA
    GAGAAAGCTA CAAAAACTCG ACTCAAACTT ATAAAAGCAA AAGCTTTAGC ATATGTTGAA
    GAGTTGACAA TGAAAGCAGA AGAAGCTTAT AAAGATATGG AAAAGTGGCT TGGAGAAAGG
    TTCCTAGCAG AAATGTCCAG TGTTGAAAAG CTGATTGAGG TAGGACGACA CCATATTGAA
    AGCTCTAGCA AAATCCAGTA TGAAATGAAT TTAGAAGAAA CAGATTTCTT CATCAGCAGT
    GATGTAAAAG TGATTCCTGA TCCTGTTCCT CCACCACAAG TTCCACTAAT AGAGACTTCT
    GAAAGTAGTA CTCTAACTAT TTCGCAACTT AACACATTTC ATAAACATTT TCTACAAGTG
    GCACCTAAAG CCATAAGAGC ACTGAAGCAA TTTAAAGGCA GGAGTAATTC TTCTAGCAAG
    AGAGAAGTTG ATGACAGAGG GAAGGAAAAG AAAGATTTGG AACATCCAAG AATAAGGAAG
    AATCGCCGGT TTCCAAAAAT ATGGCTAACA AAATACTCCT GGTTAAAATA TGATGACGAA
    AGGGGAATAA TGTTCTGTGC ATTATGTCGC AAGCATAATG TGGACCTGGG GGAAAGCATT
    CATAATTTTT GTTCTGGTAC TGATGACTTC AAACTGGAAT TTATAAATAC ACACCAGAAC
    AGTGAAGCTC ATGCCTGGGC TACCTGCATG GAGGCTGCCA GTACTGCTTC ACCAAATACA
    GTTTCTGCTG AGCAGACATT GAAGAGTATG AACTCCATAA CAATAGGAAG AGTAGAAAGT
    ACTTTTAGAA CATGCCATGC AATTGCTAAA TCTGTTCAGC CCTTTACAGA CCTGGACTGG
    ATTTATAAAT CAGATGATGT GAAAGGAACA GATATTGGTT CTCTATTCAG AAATTATAAG
    TCAGCAAGAA TGTTTAAACA TTTTATGGCT GAAGCAGAAA GAAGAGCTCT AAAAGAGGAA
    CTGGAGAATT GTAAATTCTT TTCTCTTATT AGTGATGAAT TCACAGATAG TATATCCAAA
    TCAGCTGCAG TTGTCTATGT GCACTTTGCA AATGAAGGAA AAGTTCACTG CCAGATTGTT
    GGGGTTCAAC CTGTTCAAAA AAATGATGCT TCAACTATAA AAAGTGCAAT TGAGGAAACA
    TTTCAAATAA ATCTTCAGCT TAGCTTGGCA AGCCAAGACT GGTCAAGAAA ATTAGTTGGA
    TTTGGAAGCG AGGAGACAGA TGTAATGACT GGTCAAAATA ATGGGGTAGC TAATCTCATG
    AGGGAAATTC AACCTTGTGT GCAGTCTGTG CATTGTTTTG CTCACCGCCT AAGGCTGGCA
    TACAAGGGAG CACTGGAAAA CATTCAGCTA TACAGCTTAA CCAATGGCTT GAGAAGTATG
    TACTACTTTT ATCATAACTC AACTCTAAAT AAGAAGAATC TCAAAGCTAT ATATGAAGCC
    ATCAAGTTAC ATCCAGCTAT TCCATCTTGC ACTGGGGGCT CCCAGTGGTT TTCTCGCCTA
    CAGACAGCTC TACAAATTCT CCTTAAAGGT CATCCTGCAT TTGTTCTACA TCTGGATAAG
    ACTAAAAGAG ATTCAAGGTC CTCAAATAGG CAGAAAGTCA AAGGACTATT GTATCTCCTT
    CTGAAAATGG AGATAGTCAA GTTTTCCCAT TTCTTGTTGG ATGTCATCAA TGTCCTCAGC
    ATTCTGTTAC ATATTACTGT GGATCACAAT TCCTCCATTG CAGATATATT TGCAACTATG
    CAGTCAACTG TGGAAACACT CCAAATGTAT CAAGCAAGAG CTGGTCCAAT GGAACGCTTA
    ATGAAGGCCA TTCAACATTT TCATGGTCAC CAGCTTGTTG GAAATGGAAA TATTTCAGCA
    GTACGAACCA AAGTACCAAG TAATGTGGTG AAGAGGTTAA GTGATTGTTT CTGTGATGCT
    AATCAAGGTG TCTTGAAAGC AACTCTTATT GGAAGTTTTA ATCTATGGCC TGACAAAATG
    AAACAAGTTA TAGTATTTGG TGAAAAGGAA GTATCTGTCT TGAGTAAACA TTATGAAGCC
    ATACTAGAAG CTGCTAGTGT GAAGATCAGT GAAGTTGAAA TTGAATGGAG CATGTTGAAA
    TTAGAGCTAT ATAACAGATT TCAGAACATC CAGACCTTGA CATGGGATTC GGTGAATGCA
    CATTATTCAA AGAAGTATCC CAGTATCCTG GCATTGGTAG ATCTGATCCT AACTCTGCCA
    GCAAGCTCAG CTGAAATAGA CCAAGGCTCT GGAAAAGAAA GTCATCATGA TGCATCTGCA
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153020
    Clone ID Related Accession (Same CDS sequence) XM_025144813.1
    Accession Version XM_025144813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA 
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGCCATA AGAGCACTGA AGCAATTTAA AGGCAGGAGT
    AATTCTTCTA GCAAGAGAGA AGTTGATGAC AGAGGGAAGG AAAAGAAAGA TTTGGAACAT
    CCAAGAATAA GGAAGAATCG CCGGTTTCCA AAAATATGGC TAACAAAATA CTCCTGGTTA
    AAATATGATG ACGAAAGGGG AATAATGTTC TGTGCATTAT GTCGCAAGCA TAATGTGGAC
    CTGGGGGAAA GCATTCATAA TTTTTGTTCT GGTACTGATG ACTTCAAACT GGAATTTATA
    AATACACACC AGAACAGTGA AGCTCATGCC TGGGCTACCT GCATGGAGGC TGCCAGTACT
    GCTTCACCAA ATACAGTTTC TGCTGAGCAG ACATTGAAGA GTATGAACTC CATAACAATA
    GGAAGAGTAG AAAGTACTTT TAGAACATGC CATGCAATTG CTAAATCTGT TCAGCCCTTT
    ACAGACCTGG ACTGGATTTA TAAATCAGAT GATGTGAAAG GAACAGATAT TGGTTCTCTA
    TTCAGAAATT ATAAGTCAGC AAGAATGTTT AAACATTTTA TGGCTGAAGC AGAAAGAAGA
    GCTCTAAAAG AGGAACTGGA GAATTGTAAA TTCTTTTCTC TTATTAGTGA TGAATTCACA
    GATAGTATAT CCAAATCAGC TGCAGTTGTC TATGTGCACT TTGCAAATGA AGGAAAAGTT
    CACTGCCAGA TTGTTGGGGT TCAACCTGTT CAAAAAAATG ATGCTTCAAC TATAAAAAGT
    GCAATTGAGG AAACATTTCA AATAAATCTT CAGCTTAGCT TGGCAAGCCA AGACTGGTCA
    AGAAAATTAG TTGGATTTGG AAGCGAGGAG ACAGATGTAA TGACTGGTCA AAATAATGGG
    GTAGCTAATC TCATGAGGGA AATTCAACCT TGTGTGCAGT CTGTGCATTG TTTTGCTCAC
    CGCCTAAGGC TGGCATACAA GGGAGCACTG GAAAACATTC AGCTATACAG CTTAACCAAT
    GGCTTGAGAA GTATGTACTA CTTTTATCAT AACTCAACTC TAAATAAGAA GAATCTCAAA
    GCTATATATG AAGCCATCAA GTTACATCCA GCTATTCCAT CTTGCACTGG GGGCTCCCAG
    TGGTTTTCTC GCCTACAGAC AGCTCTACAA ATTCTCCTTA AAGGTCATCC TGCATTTGTT
    CTACATCTGG ATAAGACTAA AAGAGATTCA AGGTCCTCAA ATAGGCAGAA AGTCAAAGGA
    CTATTGTATC TCCTTCTGAA AATGGAGATA GTCAAGTTTT CCCATTTCTT GTTGGATGTC
    ATCAATGTCC TCAGCATTCT GTTACATATT ACTGTGGATC ACAATTCCTC CATTGCAGAT
    ATATTTGCAA CTATGCAGTC AACTGTGGAA ACACTCCAAA TGTATCAAGC AAGAGCTGGT
    CCAATGGAAC GCTTAATGAA GGCCATTCAA CATTTTCATG GTCACCAGCT TGTTGGAAAT
    GGAAATATTT CAGCAGTACG AACCAAAGTA CCAAGTAATG TGGTGAAGAG GTTAAGTGAT
    TGTTTCTGTG ATGCTAATCA AGGTGTCTTG AAAGCAACTC TTATTGGAAG TTTTAATCTA
    TGGCCTGACA AAATGAAACA AGTTATAGTA TTTGGTGAAA AGGAAGTATC TGTCTTGAGT
    AAACATTATG AAGCCATACT AGAAGCTGCT AGTGTGAAGA TCAGTGAAGT TGAAATTGAA
    TGGAGCATGT TGAAATTAGA GCTATATAAC AGATTTCAGA ACATCCAGAC CTTGACATGG
    GATTCGGTGA ATGCACATTA TTCAAAGAAG TATCCCAGTA TCCTGGCATT GGTAGATCTG
    ATCCTAACTC TGCCAGCAAG CTCAGCTGAA ATAGACCAAG GCTCTGGAAA AGAAAGTCAT
    CATGATGCAT CTGCACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000581.1
    CDS171..5831
    Translation

    Target ORF information:

    RefSeq Version XM_025144813.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA 
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGCCATA AGAGCACTGA AGCAATTTAA AGGCAGGAGT
    AATTCTTCTA GCAAGAGAGA AGTTGATGAC AGAGGGAAGG AAAAGAAAGA TTTGGAACAT
    CCAAGAATAA GGAAGAATCG CCGGTTTCCA AAAATATGGC TAACAAAATA CTCCTGGTTA
    AAATATGATG ACGAAAGGGG AATAATGTTC TGTGCATTAT GTCGCAAGCA TAATGTGGAC
    CTGGGGGAAA GCATTCATAA TTTTTGTTCT GGTACTGATG ACTTCAAACT GGAATTTATA
    AATACACACC AGAACAGTGA AGCTCATGCC TGGGCTACCT GCATGGAGGC TGCCAGTACT
    GCTTCACCAA ATACAGTTTC TGCTGAGCAG ACATTGAAGA GTATGAACTC CATAACAATA
    GGAAGAGTAG AAAGTACTTT TAGAACATGC CATGCAATTG CTAAATCTGT TCAGCCCTTT
    ACAGACCTGG ACTGGATTTA TAAATCAGAT GATGTGAAAG GAACAGATAT TGGTTCTCTA
    TTCAGAAATT ATAAGTCAGC AAGAATGTTT AAACATTTTA TGGCTGAAGC AGAAAGAAGA
    GCTCTAAAAG AGGAACTGGA GAATTGTAAA TTCTTTTCTC TTATTAGTGA TGAATTCACA
    GATAGTATAT CCAAATCAGC TGCAGTTGTC TATGTGCACT TTGCAAATGA AGGAAAAGTT
    CACTGCCAGA TTGTTGGGGT TCAACCTGTT CAAAAAAATG ATGCTTCAAC TATAAAAAGT
    GCAATTGAGG AAACATTTCA AATAAATCTT CAGCTTAGCT TGGCAAGCCA AGACTGGTCA
    AGAAAATTAG TTGGATTTGG AAGCGAGGAG ACAGATGTAA TGACTGGTCA AAATAATGGG
    GTAGCTAATC TCATGAGGGA AATTCAACCT TGTGTGCAGT CTGTGCATTG TTTTGCTCAC
    CGCCTAAGGC TGGCATACAA GGGAGCACTG GAAAACATTC AGCTATACAG CTTAACCAAT
    GGCTTGAGAA GTATGTACTA CTTTTATCAT AACTCAACTC TAAATAAGAA GAATCTCAAA
    GCTATATATG AAGCCATCAA GTTACATCCA GCTATTCCAT CTTGCACTGG GGGCTCCCAG
    TGGTTTTCTC GCCTACAGAC AGCTCTACAA ATTCTCCTTA AAGGTCATCC TGCATTTGTT
    CTACATCTGG ATAAGACTAA AAGAGATTCA AGGTCCTCAA ATAGGCAGAA AGTCAAAGGA
    CTATTGTATC TCCTTCTGAA AATGGAGATA GTCAAGTTTT CCCATTTCTT GTTGGATGTC
    ATCAATGTCC TCAGCATTCT GTTACATATT ACTGTGGATC ACAATTCCTC CATTGCAGAT
    ATATTTGCAA CTATGCAGTC AACTGTGGAA ACACTCCAAA TGTATCAAGC AAGAGCTGGT
    CCAATGGAAC GCTTAATGAA GGCCATTCAA CATTTTCATG GTCACCAGCT TGTTGGAAAT
    GGAAATATTT CAGCAGTACG AACCAAAGTA CCAAGTAATG TGGTGAAGAG GTTAAGTGAT
    TGTTTCTGTG ATGCTAATCA AGGTGTCTTG AAAGCAACTC TTATTGGAAG TTTTAATCTA
    TGGCCTGACA AAATGAAACA AGTTATAGTA TTTGGTGAAA AGGAAGTATC TGTCTTGAGT
    AAACATTATG AAGCCATACT AGAAGCTGCT AGTGTGAAGA TCAGTGAAGT TGAAATTGAA
    TGGAGCATGT TGAAATTAGA GCTATATAAC AGATTTCAGA ACATCCAGAC CTTGACATGG
    GATTCGGTGA ATGCACATTA TTCAAAGAAG TATCCCAGTA TCCTGGCATT GGTAGATCTG
    ATCCTAACTC TGCCAGCAAG CTCAGCTGAA ATAGACCAAG GCTCTGGAAA AGAAAGTCAT
    CATGATGCAT CTGCACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153021
    Clone ID Related Accession (Same CDS sequence) XM_025144814.1 , XM_025144815.1
    Accession Version XM_025144814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTCTTCA GGATGACTTT AATCAATTTT CACAGAGCAG AACAACAGAA ATCTCTAAAG 
    CCTCAAGAGG AACAAAGAGC CCAAGACTCT GAAAAGCATC GCATAGGAAG AAGACAAAAT
    GAAATAATGG CCCGGATTCA AACAGCCGTT ATACAAGTTC CAAAGCCATC ACTAAGCCAC
    ACCTTAAAAA GTAACGAAGC AAAAAAAATG CTAAAGAAGA AAAGAGAAGC GGAGGACACT
    TACAAGGAGA TTAACAAGTT TGAGAAGTCC CTGGTAGGAA ATGCTTTTGA AGTACACAGC
    CAAGTCACTG ATAGTGAAAT ACAAGAATCA TTGCAAACAC AGAAGTTGCA AGAAACAACC
    CTAGAAACTA CAGCACAGGC AGCAACTGAG CTATTACCTA CTTATTCTGA TGATGACTAT
    GTAAGAAAAA TTCAAAAATA TTTGGAGGAA GATATGTTTG CTAGAGAACA ACGAGAAAAG
    AGACGTCGTA AAATGTTAAT GGAACAGTTG ATAGCACATG AAGCAGAAGA GGAGATTTAC
    CAGGAGGAGC AACTTGTTTA CAGGCTAATG AGACAATCTC AGCAGGAGCG GAGGATTGCT
    GTTCAACTCA TGCATGTTCG ACATGAGAAA GAGGTGTTAA AACAAAATAG AATTTTCAGG
    GAAAAGCAAT ATGAAGAAAG ACGACTGAAA GAGTTCCAGG AAGCTCTTGA CAGAGAGGCG
    GCTCTTGCAA AACAAGAAAA AATTGATTAT GAAGAACAAA GTGTCAAGGA AAGAGAGCGT
    CATGAGAAAA TTGCCATTGA AAGAGCTCAA GCTCGTTATA AAAAGCATTA TTCTGCATGT
    TGGGAAGTGA TTGATCAGAT TGTTGATTTG TCAACAAAAA TAGGAGAGTA TCGAGTATTG
    ACAAACAACA AAGTTCCGTT AAAACTGATC TGTGATTGGA AGGAATTATT TTTTAATGGA
    AACTCAATAT ATGAAAAAGC CTCAATTAAA ACTTTGCCAA GTGAACCAAG CCCAGAACAA
    CTTCTGGAGT TGAATAAAAT GGATCTATTA GATGAAAAAG ATTATGATGA ATACAAGAAT
    ATGACAGGGG AATGGTGCCC AACTGAAGAG AACAGAGAAA ATAAATCTCT TAATAATAAC
    ATATTGGGTC ATGTTCTTCA TAGACTGATG GAGATTTTCT ATCCTTCAAA ACCCAGATGT
    ACACTACCTG TACTTCCTTC ATTTCCCATC AAGGGATGTA TTCTGGGAAA AGTTTTTAGT
    GGAAAAACTA CCTGTGTAAA GTTTCTTGAA AAAGTTTGCA ATATTCAGGT ACTGTCTGTT
    GATACTCTGG TTCAGGAGGC CATCCAAGCT TTTCTTAATA ATGAAATGAA AACTGAACAC
    AGCTTGATTT CACAGGAAGC AGAGAGTTCT GCAAAACAAT ACAAGGTCCA GAGGAACTTC
    TCAGAAACAT CAATGAAATA TCTAACAGAC ACATTTACTA ATGGAACCAA AGGTAATCAT
    CCTTTCAAAA AGCACCCATC TGATCAGGAA ACTGTATCAG ACCACAAAGA GAATGACATA
    TCCAAGCTAT CTGTACGTGC TCAACTTGGT GCTGCATCTC AGAAATTGTT GAAGAAAGGA
    AAAAGTATTC CTGATGAATT ACTAGTTGAC ATCCTATTGG AAGCCATTAA GCAAACACCA
    CCAGAGAAAG GATGGATTCT GGATGGATTT CCAACGACAA TTAATCAGGC AAGGCTATTT
    GAAAAAGCCT GTACAGGGAT TGATCCAGAT GAAACAGAAG CAAAATATGT GAACTGTGGA
    AAGCTCTCAC TTGTAACAAA TCCTAGAACA CCTAGCAAGC CTCCTGTCAC CTTACCAGCA
    TTTGATGTTT CTATATTATT GGATATTTCA GACACCACAG TACTGAAACG TGTGGCTAGC
    TTGATGTCAG ATAAATCAAA GCCCTCTGAA ATTGAACAGG AGGACAGCAG TCAAAATTCA
    GATGTTAAAA TCGTAGAAGA GAAGACTGAC TTAGCAAGGG ACCAGGTGCT GCATAGACTT
    TCAGGCTTCC TAGACACATG GCCAAAATTA GAGAACTGGT TTTCAGACCA TCAGAATACT
    CTAGTGAAGG TCAATGCAGA GATGGAAGAA AGTACTGTGT GCAAAACTGT AAAGGAAATT
    CTGATTGAAG AAATTTACAA GAAGCAGAAT AGAAGGTATG TACAAGAAAA ATCACCAGAA
    GAAAAGGCTT TGCCAGTTAC ACCTCAAGAT CTGCTTTCCC CTGTTTCTGT GACACCACTG
    ATTAAGCCAG GAACAGATGA ATGGATTTAT GTAGATGAAC CACTTCCAAA GGAGATACCT
    GATTTTTTAG TACCTTATTG GGAAATGGTA GAAAATGTGT ATGTGAATAC CATCAAAACT
    GTACTTAGAT GTCTACGGGA CGAACAGTAC TCTGTGATAT ATTATTTAGC AGATATTAGA
    AAAAATTTCC AAGATTATTT AAAGCATCCA GATCATAAGC AGGAGTTTGT ATCTCAGTGG
    CAGTCAGATT TTAATTCAAT TCCTGATGAC CTGCGGGAAG ATGAGGAAAC AAAAGCAGAA
    CTACACCAAA GAGTAACTGA CTTAAGAGAT CTTCTCTGGG ATATCTGTGA TAAAAGAAGG
    GAAGAAGCAG AGCAAGAACG TACTGATATC ATGAATGATG GCTGGTTGCG AGATCATAAA
    GGGATTGCAA TAAACCATTT TTTTTCACTT ATGCAGGTTG AAGTGGATCG TTTCCAAGAT
    ACAAAGAGAC TTCTTCATGA CTATTATAGA GCAATGGAAG GAAAAATCCC AACAGAGGAC
    AGACAAAGTT TTACTAGACT TCCATTGCTA GATATTATTG ATAAAGAGCA GCAAGAAGAT
    CAGAATGAAT CAAGAAGGAT TCCTCTAGTT TCTTATAAAC TGCCTTCACC AGAAACAAAT
    ATTACTAAGT CAAAAAGCAA GGGAAATCTA CTGAAGAGTG TTAAGGATGA GAATTCTTCT
    GAAAATGTAG TGGTCGCTCT TGGGAAAGAT GAAAATGTTA TTCGTGATAC GTGGCAAACA
    GCAGTAACAG CAGTATCAGA TATGGTAAGA GCAGAAATAC AGTCAAAAGA GGTGAAGGAA
    GAAGGAGAAA AGCAGACAGA AGTGAAAGAA GATACAAAGT CTTCTCAAAC AATATCAAGC
    AAATCTACTG GGAAGGATGC CAAAGACACC AAAAAATCTC CCAAATCACC TACTAAAACG
    AAAGGACCTC GCTCCACTGC ATCTGCAGCT GAAGACAGTC CAGTTCCGTT AACCGCTGAA
    GAACTGAAGA AACAAAAACT GATACTTAAA ATACAGCAAG AATATTTTGC TGCCCTGAAA
    CATGAAGAGA AAGCTACAAA AACTCGACTC AAACTTATAA AAGCAAAAGC TTTAGCATAT
    GTTGAAGAGT TGACAATGAA AGCAGAAGAA GCTTATAAAG ATATGGAAAA GTGGCTTGGA
    GAAAGGTTCC TAGCAGAAAT GTCCAGTGTT GAAAAGCTGA TTGAGGTAGG ACGACACCAT
    ATTGAAAGCT CTAGCAAAAT CCAGTATGAA ATGAATTTAG AAGAAACAGA TTTCTTCATC
    AGCAGTGATG TAAAAGTGAT TCCTGATCCT GTTCCTCCAC CACAAGTTCC ACTAATAGAG
    ACTTCTGAAA GTAGTACTCT AACTATTTCG CAACTTAACA CATTTCATAA ACATTTTCTA
    CAAGTGGCAC CTAAAGCCAT AAGAGCACTG AAGCAATTTA AAGGCAGGAG TAATTCTTCT
    AGCAAGAGAG AAGTTGATGA CAGAGGGAAG GAAAAGAAAG ATTTGGAACA TCCAAGAATA
    AGGAAGAATC GCCGGTTTCC AAAAATATGG CTAACAAAAT ACTCCTGGTT AAAATATGAT
    GACGAAAGGG GAATAATGTT CTGTGCATTA TGTCGCAAGC ATAATGTGGA CCTGGGGGAA
    AGCATTCATA ATTTTTGTTC TGGTACTGAT GACTTCAAAC TGGAATTTAT AAATACACAC
    CAGAACAGTG AAGCTCATGC CTGGGCTACC TGCATGGAGG CTGCCAGTAC TGCTTCACCA
    AATACAGTTT CTGCTGAGCA GACATTGAAG AGTATGAACT CCATAACAAT AGGAAGAGTA
    GAAAGTACTT TTAGAACATG CCATGCAATT GCTAAATCTG TTCAGCCCTT TACAGACCTG
    GACTGGATTT ATAAATCAGA TGATGTGAAA GGAACAGATA TTGGTTCTCT ATTCAGAAAT
    TATAAGTCAG CAAGAATGTT TAAACATTTT ATGGCTGAAG CAGAAAGAAG AGCTCTAAAA
    GAGGAACTGG AGAATTGTAA ATTCTTTTCT CTTATTAGTG ATGAATTCAC AGATAGTATA
    TCCAAATCAG CTGCAGTTGT CTATGTGCAC TTTGCAAATG AAGGAAAAGT TCACTGCCAG
    ATTGTTGGGG TTCAACCTGT TCAAAAAAAT GATGCTTCAA CTATAAAAAG TGCAATTGAG
    GAAACATTTC AAATAAATCT TCAGCTTAGC TTGGCAAGCC AAGACTGGTC AAGAAAATTA
    GTTGGATTTG GAAGCGAGGA GACAGATGTA ATGACTGGTC AAAATAATGG GGTAGCTAAT
    CTCATGAGGG AAATTCAACC TTGTGTGCAG TCTGTGCATT GTTTTGCTCA CCGCCTAAGG
    CTGGCATACA AGGGAGCACT GGAAAACATT CAGCTATACA GCTTAACCAA TGGCTTGAGA
    AGTATGTACT ACTTTTATCA TAACTCAACT CTAAATAAGA AGAATCTCAA AGCTATATAT
    GAAGCCATCA AGTTACATCC AGCTATTCCA TCTTGCACTG GGGGCTCCCA GTGGTTTTCT
    CGCCTACAGA CAGCTCTACA AATTCTCCTT AAAGGTCATC CTGCATTTGT TCTACATCTG
    GATAAGACTA AAAGAGATTC AAGGTCCTCA AATAGGCAGA AAGTCAAAGG ACTATTGTAT
    CTCCTTCTGA AAATGGAGAT AGTCAAGTTT TCCCATTTCT TGTTGGATGT CATCAATGTC
    CTCAGCATTC TGTTACATAT TACTGTGGAT CACAATTCCT CCATTGCAGA TATATTTGCA
    ACTATGCAGT CAACTGTGGA AACACTCCAA ATGTATCAAG CAAGAGCTGG TCCAATGGAA
    CGCTTAATGA AGGCCATTCA ACATTTTCAT GGTCACCAGC TTGTTGGAAA TGGAAATATT
    TCAGCAGTAC GAACCAAAGT ACCAAGTAAT GTGGTGAAGA GGTTAAGTGA TTGTTTCTGT
    GATGCTAATC AAGGTGTCTT GAAAGCAACT CTTATTGGAA GTTTTAATCT ATGGCCTGAC
    AAAATGAAAC AAGTTATAGT ATTTGGTGAA AAGGAAGTAT CTGTCTTGAG TAAACATTAT
    GAAGCCATAC TAGAAGCTGC TAGTGTGAAG ATCAGTGAAG TTGAAATTGA ATGGAGCATG
    TTGAAATTAG AGCTATATAA CAGATTTCAG AACATCCAGA CCTTGACATG GGATTCGGTG
    AATGCACATT ATTCAAAGAA GTATCCCAGT ATCCTGGCAT TGGTAGATCT GATCCTAACT
    CTGCCAGCAA GCTCAGCTGA AATAGACCAA GGCTCTGGAA AAGAAAGTCA TCATGATGCA
    TCTGCACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000582.1
    CDS197..5848
    Translation

    Target ORF information:

    RefSeq Version XM_025144814.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTCTTCA GGATGACTTT AATCAATTTT CACAGAGCAG AACAACAGAA ATCTCTAAAG 
    CCTCAAGAGG AACAAAGAGC CCAAGACTCT GAAAAGCATC GCATAGGAAG AAGACAAAAT
    GAAATAATGG CCCGGATTCA AACAGCCGTT ATACAAGTTC CAAAGCCATC ACTAAGCCAC
    ACCTTAAAAA GTAACGAAGC AAAAAAAATG CTAAAGAAGA AAAGAGAAGC GGAGGACACT
    TACAAGGAGA TTAACAAGTT TGAGAAGTCC CTGGTAGGAA ATGCTTTTGA AGTACACAGC
    CAAGTCACTG ATAGTGAAAT ACAAGAATCA TTGCAAACAC AGAAGTTGCA AGAAACAACC
    CTAGAAACTA CAGCACAGGC AGCAACTGAG CTATTACCTA CTTATTCTGA TGATGACTAT
    GTAAGAAAAA TTCAAAAATA TTTGGAGGAA GATATGTTTG CTAGAGAACA ACGAGAAAAG
    AGACGTCGTA AAATGTTAAT GGAACAGTTG ATAGCACATG AAGCAGAAGA GGAGATTTAC
    CAGGAGGAGC AACTTGTTTA CAGGCTAATG AGACAATCTC AGCAGGAGCG GAGGATTGCT
    GTTCAACTCA TGCATGTTCG ACATGAGAAA GAGGTGTTAA AACAAAATAG AATTTTCAGG
    GAAAAGCAAT ATGAAGAAAG ACGACTGAAA GAGTTCCAGG AAGCTCTTGA CAGAGAGGCG
    GCTCTTGCAA AACAAGAAAA AATTGATTAT GAAGAACAAA GTGTCAAGGA AAGAGAGCGT
    CATGAGAAAA TTGCCATTGA AAGAGCTCAA GCTCGTTATA AAAAGCATTA TTCTGCATGT
    TGGGAAGTGA TTGATCAGAT TGTTGATTTG TCAACAAAAA TAGGAGAGTA TCGAGTATTG
    ACAAACAACA AAGTTCCGTT AAAACTGATC TGTGATTGGA AGGAATTATT TTTTAATGGA
    AACTCAATAT ATGAAAAAGC CTCAATTAAA ACTTTGCCAA GTGAACCAAG CCCAGAACAA
    CTTCTGGAGT TGAATAAAAT GGATCTATTA GATGAAAAAG ATTATGATGA ATACAAGAAT
    ATGACAGGGG AATGGTGCCC AACTGAAGAG AACAGAGAAA ATAAATCTCT TAATAATAAC
    ATATTGGGTC ATGTTCTTCA TAGACTGATG GAGATTTTCT ATCCTTCAAA ACCCAGATGT
    ACACTACCTG TACTTCCTTC ATTTCCCATC AAGGGATGTA TTCTGGGAAA AGTTTTTAGT
    GGAAAAACTA CCTGTGTAAA GTTTCTTGAA AAAGTTTGCA ATATTCAGGT ACTGTCTGTT
    GATACTCTGG TTCAGGAGGC CATCCAAGCT TTTCTTAATA ATGAAATGAA AACTGAACAC
    AGCTTGATTT CACAGGAAGC AGAGAGTTCT GCAAAACAAT ACAAGGTCCA GAGGAACTTC
    TCAGAAACAT CAATGAAATA TCTAACAGAC ACATTTACTA ATGGAACCAA AGGTAATCAT
    CCTTTCAAAA AGCACCCATC TGATCAGGAA ACTGTATCAG ACCACAAAGA GAATGACATA
    TCCAAGCTAT CTGTACGTGC TCAACTTGGT GCTGCATCTC AGAAATTGTT GAAGAAAGGA
    AAAAGTATTC CTGATGAATT ACTAGTTGAC ATCCTATTGG AAGCCATTAA GCAAACACCA
    CCAGAGAAAG GATGGATTCT GGATGGATTT CCAACGACAA TTAATCAGGC AAGGCTATTT
    GAAAAAGCCT GTACAGGGAT TGATCCAGAT GAAACAGAAG CAAAATATGT GAACTGTGGA
    AAGCTCTCAC TTGTAACAAA TCCTAGAACA CCTAGCAAGC CTCCTGTCAC CTTACCAGCA
    TTTGATGTTT CTATATTATT GGATATTTCA GACACCACAG TACTGAAACG TGTGGCTAGC
    TTGATGTCAG ATAAATCAAA GCCCTCTGAA ATTGAACAGG AGGACAGCAG TCAAAATTCA
    GATGTTAAAA TCGTAGAAGA GAAGACTGAC TTAGCAAGGG ACCAGGTGCT GCATAGACTT
    TCAGGCTTCC TAGACACATG GCCAAAATTA GAGAACTGGT TTTCAGACCA TCAGAATACT
    CTAGTGAAGG TCAATGCAGA GATGGAAGAA AGTACTGTGT GCAAAACTGT AAAGGAAATT
    CTGATTGAAG AAATTTACAA GAAGCAGAAT AGAAGGTATG TACAAGAAAA ATCACCAGAA
    GAAAAGGCTT TGCCAGTTAC ACCTCAAGAT CTGCTTTCCC CTGTTTCTGT GACACCACTG
    ATTAAGCCAG GAACAGATGA ATGGATTTAT GTAGATGAAC CACTTCCAAA GGAGATACCT
    GATTTTTTAG TACCTTATTG GGAAATGGTA GAAAATGTGT ATGTGAATAC CATCAAAACT
    GTACTTAGAT GTCTACGGGA CGAACAGTAC TCTGTGATAT ATTATTTAGC AGATATTAGA
    AAAAATTTCC AAGATTATTT AAAGCATCCA GATCATAAGC AGGAGTTTGT ATCTCAGTGG
    CAGTCAGATT TTAATTCAAT TCCTGATGAC CTGCGGGAAG ATGAGGAAAC AAAAGCAGAA
    CTACACCAAA GAGTAACTGA CTTAAGAGAT CTTCTCTGGG ATATCTGTGA TAAAAGAAGG
    GAAGAAGCAG AGCAAGAACG TACTGATATC ATGAATGATG GCTGGTTGCG AGATCATAAA
    GGGATTGCAA TAAACCATTT TTTTTCACTT ATGCAGGTTG AAGTGGATCG TTTCCAAGAT
    ACAAAGAGAC TTCTTCATGA CTATTATAGA GCAATGGAAG GAAAAATCCC AACAGAGGAC
    AGACAAAGTT TTACTAGACT TCCATTGCTA GATATTATTG ATAAAGAGCA GCAAGAAGAT
    CAGAATGAAT CAAGAAGGAT TCCTCTAGTT TCTTATAAAC TGCCTTCACC AGAAACAAAT
    ATTACTAAGT CAAAAAGCAA GGGAAATCTA CTGAAGAGTG TTAAGGATGA GAATTCTTCT
    GAAAATGTAG TGGTCGCTCT TGGGAAAGAT GAAAATGTTA TTCGTGATAC GTGGCAAACA
    GCAGTAACAG CAGTATCAGA TATGGTAAGA GCAGAAATAC AGTCAAAAGA GGTGAAGGAA
    GAAGGAGAAA AGCAGACAGA AGTGAAAGAA GATACAAAGT CTTCTCAAAC AATATCAAGC
    AAATCTACTG GGAAGGATGC CAAAGACACC AAAAAATCTC CCAAATCACC TACTAAAACG
    AAAGGACCTC GCTCCACTGC ATCTGCAGCT GAAGACAGTC CAGTTCCGTT AACCGCTGAA
    GAACTGAAGA AACAAAAACT GATACTTAAA ATACAGCAAG AATATTTTGC TGCCCTGAAA
    CATGAAGAGA AAGCTACAAA AACTCGACTC AAACTTATAA AAGCAAAAGC TTTAGCATAT
    GTTGAAGAGT TGACAATGAA AGCAGAAGAA GCTTATAAAG ATATGGAAAA GTGGCTTGGA
    GAAAGGTTCC TAGCAGAAAT GTCCAGTGTT GAAAAGCTGA TTGAGGTAGG ACGACACCAT
    ATTGAAAGCT CTAGCAAAAT CCAGTATGAA ATGAATTTAG AAGAAACAGA TTTCTTCATC
    AGCAGTGATG TAAAAGTGAT TCCTGATCCT GTTCCTCCAC CACAAGTTCC ACTAATAGAG
    ACTTCTGAAA GTAGTACTCT AACTATTTCG CAACTTAACA CATTTCATAA ACATTTTCTA
    CAAGTGGCAC CTAAAGCCAT AAGAGCACTG AAGCAATTTA AAGGCAGGAG TAATTCTTCT
    AGCAAGAGAG AAGTTGATGA CAGAGGGAAG GAAAAGAAAG ATTTGGAACA TCCAAGAATA
    AGGAAGAATC GCCGGTTTCC AAAAATATGG CTAACAAAAT ACTCCTGGTT AAAATATGAT
    GACGAAAGGG GAATAATGTT CTGTGCATTA TGTCGCAAGC ATAATGTGGA CCTGGGGGAA
    AGCATTCATA ATTTTTGTTC TGGTACTGAT GACTTCAAAC TGGAATTTAT AAATACACAC
    CAGAACAGTG AAGCTCATGC CTGGGCTACC TGCATGGAGG CTGCCAGTAC TGCTTCACCA
    AATACAGTTT CTGCTGAGCA GACATTGAAG AGTATGAACT CCATAACAAT AGGAAGAGTA
    GAAAGTACTT TTAGAACATG CCATGCAATT GCTAAATCTG TTCAGCCCTT TACAGACCTG
    GACTGGATTT ATAAATCAGA TGATGTGAAA GGAACAGATA TTGGTTCTCT ATTCAGAAAT
    TATAAGTCAG CAAGAATGTT TAAACATTTT ATGGCTGAAG CAGAAAGAAG AGCTCTAAAA
    GAGGAACTGG AGAATTGTAA ATTCTTTTCT CTTATTAGTG ATGAATTCAC AGATAGTATA
    TCCAAATCAG CTGCAGTTGT CTATGTGCAC TTTGCAAATG AAGGAAAAGT TCACTGCCAG
    ATTGTTGGGG TTCAACCTGT TCAAAAAAAT GATGCTTCAA CTATAAAAAG TGCAATTGAG
    GAAACATTTC AAATAAATCT TCAGCTTAGC TTGGCAAGCC AAGACTGGTC AAGAAAATTA
    GTTGGATTTG GAAGCGAGGA GACAGATGTA ATGACTGGTC AAAATAATGG GGTAGCTAAT
    CTCATGAGGG AAATTCAACC TTGTGTGCAG TCTGTGCATT GTTTTGCTCA CCGCCTAAGG
    CTGGCATACA AGGGAGCACT GGAAAACATT CAGCTATACA GCTTAACCAA TGGCTTGAGA
    AGTATGTACT ACTTTTATCA TAACTCAACT CTAAATAAGA AGAATCTCAA AGCTATATAT
    GAAGCCATCA AGTTACATCC AGCTATTCCA TCTTGCACTG GGGGCTCCCA GTGGTTTTCT
    CGCCTACAGA CAGCTCTACA AATTCTCCTT AAAGGTCATC CTGCATTTGT TCTACATCTG
    GATAAGACTA AAAGAGATTC AAGGTCCTCA AATAGGCAGA AAGTCAAAGG ACTATTGTAT
    CTCCTTCTGA AAATGGAGAT AGTCAAGTTT TCCCATTTCT TGTTGGATGT CATCAATGTC
    CTCAGCATTC TGTTACATAT TACTGTGGAT CACAATTCCT CCATTGCAGA TATATTTGCA
    ACTATGCAGT CAACTGTGGA AACACTCCAA ATGTATCAAG CAAGAGCTGG TCCAATGGAA
    CGCTTAATGA AGGCCATTCA ACATTTTCAT GGTCACCAGC TTGTTGGAAA TGGAAATATT
    TCAGCAGTAC GAACCAAAGT ACCAAGTAAT GTGGTGAAGA GGTTAAGTGA TTGTTTCTGT
    GATGCTAATC AAGGTGTCTT GAAAGCAACT CTTATTGGAA GTTTTAATCT ATGGCCTGAC
    AAAATGAAAC AAGTTATAGT ATTTGGTGAA AAGGAAGTAT CTGTCTTGAG TAAACATTAT
    GAAGCCATAC TAGAAGCTGC TAGTGTGAAG ATCAGTGAAG TTGAAATTGA ATGGAGCATG
    TTGAAATTAG AGCTATATAA CAGATTTCAG AACATCCAGA CCTTGACATG GGATTCGGTG
    AATGCACATT ATTCAAAGAA GTATCCCAGT ATCCTGGCAT TGGTAGATCT GATCCTAACT
    CTGCCAGCAA GCTCAGCTGA AATAGACCAA GGCTCTGGAA AAGAAAGTCA TCATGATGCA
    TCTGCACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153021
    Clone ID Related Accession (Same CDS sequence) XM_025144814.1 , XM_025144815.1
    Accession Version XM_025144815.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTCTTCA GGATGACTTT AATCAATTTT CACAGAGCAG AACAACAGAA ATCTCTAAAG 
    CCTCAAGAGG AACAAAGAGC CCAAGACTCT GAAAAGCATC GCATAGGAAG AAGACAAAAT
    GAAATAATGG CCCGGATTCA AACAGCCGTT ATACAAGTTC CAAAGCCATC ACTAAGCCAC
    ACCTTAAAAA GTAACGAAGC AAAAAAAATG CTAAAGAAGA AAAGAGAAGC GGAGGACACT
    TACAAGGAGA TTAACAAGTT TGAGAAGTCC CTGGTAGGAA ATGCTTTTGA AGTACACAGC
    CAAGTCACTG ATAGTGAAAT ACAAGAATCA TTGCAAACAC AGAAGTTGCA AGAAACAACC
    CTAGAAACTA CAGCACAGGC AGCAACTGAG CTATTACCTA CTTATTCTGA TGATGACTAT
    GTAAGAAAAA TTCAAAAATA TTTGGAGGAA GATATGTTTG CTAGAGAACA ACGAGAAAAG
    AGACGTCGTA AAATGTTAAT GGAACAGTTG ATAGCACATG AAGCAGAAGA GGAGATTTAC
    CAGGAGGAGC AACTTGTTTA CAGGCTAATG AGACAATCTC AGCAGGAGCG GAGGATTGCT
    GTTCAACTCA TGCATGTTCG ACATGAGAAA GAGGTGTTAA AACAAAATAG AATTTTCAGG
    GAAAAGCAAT ATGAAGAAAG ACGACTGAAA GAGTTCCAGG AAGCTCTTGA CAGAGAGGCG
    GCTCTTGCAA AACAAGAAAA AATTGATTAT GAAGAACAAA GTGTCAAGGA AAGAGAGCGT
    CATGAGAAAA TTGCCATTGA AAGAGCTCAA GCTCGTTATA AAAAGCATTA TTCTGCATGT
    TGGGAAGTGA TTGATCAGAT TGTTGATTTG TCAACAAAAA TAGGAGAGTA TCGAGTATTG
    ACAAACAACA AAGTTCCGTT AAAACTGATC TGTGATTGGA AGGAATTATT TTTTAATGGA
    AACTCAATAT ATGAAAAAGC CTCAATTAAA ACTTTGCCAA GTGAACCAAG CCCAGAACAA
    CTTCTGGAGT TGAATAAAAT GGATCTATTA GATGAAAAAG ATTATGATGA ATACAAGAAT
    ATGACAGGGG AATGGTGCCC AACTGAAGAG AACAGAGAAA ATAAATCTCT TAATAATAAC
    ATATTGGGTC ATGTTCTTCA TAGACTGATG GAGATTTTCT ATCCTTCAAA ACCCAGATGT
    ACACTACCTG TACTTCCTTC ATTTCCCATC AAGGGATGTA TTCTGGGAAA AGTTTTTAGT
    GGAAAAACTA CCTGTGTAAA GTTTCTTGAA AAAGTTTGCA ATATTCAGGT ACTGTCTGTT
    GATACTCTGG TTCAGGAGGC CATCCAAGCT TTTCTTAATA ATGAAATGAA AACTGAACAC
    AGCTTGATTT CACAGGAAGC AGAGAGTTCT GCAAAACAAT ACAAGGTCCA GAGGAACTTC
    TCAGAAACAT CAATGAAATA TCTAACAGAC ACATTTACTA ATGGAACCAA AGGTAATCAT
    CCTTTCAAAA AGCACCCATC TGATCAGGAA ACTGTATCAG ACCACAAAGA GAATGACATA
    TCCAAGCTAT CTGTACGTGC TCAACTTGGT GCTGCATCTC AGAAATTGTT GAAGAAAGGA
    AAAAGTATTC CTGATGAATT ACTAGTTGAC ATCCTATTGG AAGCCATTAA GCAAACACCA
    CCAGAGAAAG GATGGATTCT GGATGGATTT CCAACGACAA TTAATCAGGC AAGGCTATTT
    GAAAAAGCCT GTACAGGGAT TGATCCAGAT GAAACAGAAG CAAAATATGT GAACTGTGGA
    AAGCTCTCAC TTGTAACAAA TCCTAGAACA CCTAGCAAGC CTCCTGTCAC CTTACCAGCA
    TTTGATGTTT CTATATTATT GGATATTTCA GACACCACAG TACTGAAACG TGTGGCTAGC
    TTGATGTCAG ATAAATCAAA GCCCTCTGAA ATTGAACAGG AGGACAGCAG TCAAAATTCA
    GATGTTAAAA TCGTAGAAGA GAAGACTGAC TTAGCAAGGG ACCAGGTGCT GCATAGACTT
    TCAGGCTTCC TAGACACATG GCCAAAATTA GAGAACTGGT TTTCAGACCA TCAGAATACT
    CTAGTGAAGG TCAATGCAGA GATGGAAGAA AGTACTGTGT GCAAAACTGT AAAGGAAATT
    CTGATTGAAG AAATTTACAA GAAGCAGAAT AGAAGGTATG TACAAGAAAA ATCACCAGAA
    GAAAAGGCTT TGCCAGTTAC ACCTCAAGAT CTGCTTTCCC CTGTTTCTGT GACACCACTG
    ATTAAGCCAG GAACAGATGA ATGGATTTAT GTAGATGAAC CACTTCCAAA GGAGATACCT
    GATTTTTTAG TACCTTATTG GGAAATGGTA GAAAATGTGT ATGTGAATAC CATCAAAACT
    GTACTTAGAT GTCTACGGGA CGAACAGTAC TCTGTGATAT ATTATTTAGC AGATATTAGA
    AAAAATTTCC AAGATTATTT AAAGCATCCA GATCATAAGC AGGAGTTTGT ATCTCAGTGG
    CAGTCAGATT TTAATTCAAT TCCTGATGAC CTGCGGGAAG ATGAGGAAAC AAAAGCAGAA
    CTACACCAAA GAGTAACTGA CTTAAGAGAT CTTCTCTGGG ATATCTGTGA TAAAAGAAGG
    GAAGAAGCAG AGCAAGAACG TACTGATATC ATGAATGATG GCTGGTTGCG AGATCATAAA
    GGGATTGCAA TAAACCATTT TTTTTCACTT ATGCAGGTTG AAGTGGATCG TTTCCAAGAT
    ACAAAGAGAC TTCTTCATGA CTATTATAGA GCAATGGAAG GAAAAATCCC AACAGAGGAC
    AGACAAAGTT TTACTAGACT TCCATTGCTA GATATTATTG ATAAAGAGCA GCAAGAAGAT
    CAGAATGAAT CAAGAAGGAT TCCTCTAGTT TCTTATAAAC TGCCTTCACC AGAAACAAAT
    ATTACTAAGT CAAAAAGCAA GGGAAATCTA CTGAAGAGTG TTAAGGATGA GAATTCTTCT
    GAAAATGTAG TGGTCGCTCT TGGGAAAGAT GAAAATGTTA TTCGTGATAC GTGGCAAACA
    GCAGTAACAG CAGTATCAGA TATGGTAAGA GCAGAAATAC AGTCAAAAGA GGTGAAGGAA
    GAAGGAGAAA AGCAGACAGA AGTGAAAGAA GATACAAAGT CTTCTCAAAC AATATCAAGC
    AAATCTACTG GGAAGGATGC CAAAGACACC AAAAAATCTC CCAAATCACC TACTAAAACG
    AAAGGACCTC GCTCCACTGC ATCTGCAGCT GAAGACAGTC CAGTTCCGTT AACCGCTGAA
    GAACTGAAGA AACAAAAACT GATACTTAAA ATACAGCAAG AATATTTTGC TGCCCTGAAA
    CATGAAGAGA AAGCTACAAA AACTCGACTC AAACTTATAA AAGCAAAAGC TTTAGCATAT
    GTTGAAGAGT TGACAATGAA AGCAGAAGAA GCTTATAAAG ATATGGAAAA GTGGCTTGGA
    GAAAGGTTCC TAGCAGAAAT GTCCAGTGTT GAAAAGCTGA TTGAGGTAGG ACGACACCAT
    ATTGAAAGCT CTAGCAAAAT CCAGTATGAA ATGAATTTAG AAGAAACAGA TTTCTTCATC
    AGCAGTGATG TAAAAGTGAT TCCTGATCCT GTTCCTCCAC CACAAGTTCC ACTAATAGAG
    ACTTCTGAAA GTAGTACTCT AACTATTTCG CAACTTAACA CATTTCATAA ACATTTTCTA
    CAAGTGGCAC CTAAAGCCAT AAGAGCACTG AAGCAATTTA AAGGCAGGAG TAATTCTTCT
    AGCAAGAGAG AAGTTGATGA CAGAGGGAAG GAAAAGAAAG ATTTGGAACA TCCAAGAATA
    AGGAAGAATC GCCGGTTTCC AAAAATATGG CTAACAAAAT ACTCCTGGTT AAAATATGAT
    GACGAAAGGG GAATAATGTT CTGTGCATTA TGTCGCAAGC ATAATGTGGA CCTGGGGGAA
    AGCATTCATA ATTTTTGTTC TGGTACTGAT GACTTCAAAC TGGAATTTAT AAATACACAC
    CAGAACAGTG AAGCTCATGC CTGGGCTACC TGCATGGAGG CTGCCAGTAC TGCTTCACCA
    AATACAGTTT CTGCTGAGCA GACATTGAAG AGTATGAACT CCATAACAAT AGGAAGAGTA
    GAAAGTACTT TTAGAACATG CCATGCAATT GCTAAATCTG TTCAGCCCTT TACAGACCTG
    GACTGGATTT ATAAATCAGA TGATGTGAAA GGAACAGATA TTGGTTCTCT ATTCAGAAAT
    TATAAGTCAG CAAGAATGTT TAAACATTTT ATGGCTGAAG CAGAAAGAAG AGCTCTAAAA
    GAGGAACTGG AGAATTGTAA ATTCTTTTCT CTTATTAGTG ATGAATTCAC AGATAGTATA
    TCCAAATCAG CTGCAGTTGT CTATGTGCAC TTTGCAAATG AAGGAAAAGT TCACTGCCAG
    ATTGTTGGGG TTCAACCTGT TCAAAAAAAT GATGCTTCAA CTATAAAAAG TGCAATTGAG
    GAAACATTTC AAATAAATCT TCAGCTTAGC TTGGCAAGCC AAGACTGGTC AAGAAAATTA
    GTTGGATTTG GAAGCGAGGA GACAGATGTA ATGACTGGTC AAAATAATGG GGTAGCTAAT
    CTCATGAGGG AAATTCAACC TTGTGTGCAG TCTGTGCATT GTTTTGCTCA CCGCCTAAGG
    CTGGCATACA AGGGAGCACT GGAAAACATT CAGCTATACA GCTTAACCAA TGGCTTGAGA
    AGTATGTACT ACTTTTATCA TAACTCAACT CTAAATAAGA AGAATCTCAA AGCTATATAT
    GAAGCCATCA AGTTACATCC AGCTATTCCA TCTTGCACTG GGGGCTCCCA GTGGTTTTCT
    CGCCTACAGA CAGCTCTACA AATTCTCCTT AAAGGTCATC CTGCATTTGT TCTACATCTG
    GATAAGACTA AAAGAGATTC AAGGTCCTCA AATAGGCAGA AAGTCAAAGG ACTATTGTAT
    CTCCTTCTGA AAATGGAGAT AGTCAAGTTT TCCCATTTCT TGTTGGATGT CATCAATGTC
    CTCAGCATTC TGTTACATAT TACTGTGGAT CACAATTCCT CCATTGCAGA TATATTTGCA
    ACTATGCAGT CAACTGTGGA AACACTCCAA ATGTATCAAG CAAGAGCTGG TCCAATGGAA
    CGCTTAATGA AGGCCATTCA ACATTTTCAT GGTCACCAGC TTGTTGGAAA TGGAAATATT
    TCAGCAGTAC GAACCAAAGT ACCAAGTAAT GTGGTGAAGA GGTTAAGTGA TTGTTTCTGT
    GATGCTAATC AAGGTGTCTT GAAAGCAACT CTTATTGGAA GTTTTAATCT ATGGCCTGAC
    AAAATGAAAC AAGTTATAGT ATTTGGTGAA AAGGAAGTAT CTGTCTTGAG TAAACATTAT
    GAAGCCATAC TAGAAGCTGC TAGTGTGAAG ATCAGTGAAG TTGAAATTGA ATGGAGCATG
    TTGAAATTAG AGCTATATAA CAGATTTCAG AACATCCAGA CCTTGACATG GGATTCGGTG
    AATGCACATT ATTCAAAGAA GTATCCCAGT ATCCTGGCAT TGGTAGATCT GATCCTAACT
    CTGCCAGCAA GCTCAGCTGA AATAGACCAA GGCTCTGGAA AAGAAAGTCA TCATGATGCA
    TCTGCACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000583.1
    CDS198..5849
    Translation

    Target ORF information:

    RefSeq Version XM_025144815.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144815.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTCTTCA GGATGACTTT AATCAATTTT CACAGAGCAG AACAACAGAA ATCTCTAAAG 
    CCTCAAGAGG AACAAAGAGC CCAAGACTCT GAAAAGCATC GCATAGGAAG AAGACAAAAT
    GAAATAATGG CCCGGATTCA AACAGCCGTT ATACAAGTTC CAAAGCCATC ACTAAGCCAC
    ACCTTAAAAA GTAACGAAGC AAAAAAAATG CTAAAGAAGA AAAGAGAAGC GGAGGACACT
    TACAAGGAGA TTAACAAGTT TGAGAAGTCC CTGGTAGGAA ATGCTTTTGA AGTACACAGC
    CAAGTCACTG ATAGTGAAAT ACAAGAATCA TTGCAAACAC AGAAGTTGCA AGAAACAACC
    CTAGAAACTA CAGCACAGGC AGCAACTGAG CTATTACCTA CTTATTCTGA TGATGACTAT
    GTAAGAAAAA TTCAAAAATA TTTGGAGGAA GATATGTTTG CTAGAGAACA ACGAGAAAAG
    AGACGTCGTA AAATGTTAAT GGAACAGTTG ATAGCACATG AAGCAGAAGA GGAGATTTAC
    CAGGAGGAGC AACTTGTTTA CAGGCTAATG AGACAATCTC AGCAGGAGCG GAGGATTGCT
    GTTCAACTCA TGCATGTTCG ACATGAGAAA GAGGTGTTAA AACAAAATAG AATTTTCAGG
    GAAAAGCAAT ATGAAGAAAG ACGACTGAAA GAGTTCCAGG AAGCTCTTGA CAGAGAGGCG
    GCTCTTGCAA AACAAGAAAA AATTGATTAT GAAGAACAAA GTGTCAAGGA AAGAGAGCGT
    CATGAGAAAA TTGCCATTGA AAGAGCTCAA GCTCGTTATA AAAAGCATTA TTCTGCATGT
    TGGGAAGTGA TTGATCAGAT TGTTGATTTG TCAACAAAAA TAGGAGAGTA TCGAGTATTG
    ACAAACAACA AAGTTCCGTT AAAACTGATC TGTGATTGGA AGGAATTATT TTTTAATGGA
    AACTCAATAT ATGAAAAAGC CTCAATTAAA ACTTTGCCAA GTGAACCAAG CCCAGAACAA
    CTTCTGGAGT TGAATAAAAT GGATCTATTA GATGAAAAAG ATTATGATGA ATACAAGAAT
    ATGACAGGGG AATGGTGCCC AACTGAAGAG AACAGAGAAA ATAAATCTCT TAATAATAAC
    ATATTGGGTC ATGTTCTTCA TAGACTGATG GAGATTTTCT ATCCTTCAAA ACCCAGATGT
    ACACTACCTG TACTTCCTTC ATTTCCCATC AAGGGATGTA TTCTGGGAAA AGTTTTTAGT
    GGAAAAACTA CCTGTGTAAA GTTTCTTGAA AAAGTTTGCA ATATTCAGGT ACTGTCTGTT
    GATACTCTGG TTCAGGAGGC CATCCAAGCT TTTCTTAATA ATGAAATGAA AACTGAACAC
    AGCTTGATTT CACAGGAAGC AGAGAGTTCT GCAAAACAAT ACAAGGTCCA GAGGAACTTC
    TCAGAAACAT CAATGAAATA TCTAACAGAC ACATTTACTA ATGGAACCAA AGGTAATCAT
    CCTTTCAAAA AGCACCCATC TGATCAGGAA ACTGTATCAG ACCACAAAGA GAATGACATA
    TCCAAGCTAT CTGTACGTGC TCAACTTGGT GCTGCATCTC AGAAATTGTT GAAGAAAGGA
    AAAAGTATTC CTGATGAATT ACTAGTTGAC ATCCTATTGG AAGCCATTAA GCAAACACCA
    CCAGAGAAAG GATGGATTCT GGATGGATTT CCAACGACAA TTAATCAGGC AAGGCTATTT
    GAAAAAGCCT GTACAGGGAT TGATCCAGAT GAAACAGAAG CAAAATATGT GAACTGTGGA
    AAGCTCTCAC TTGTAACAAA TCCTAGAACA CCTAGCAAGC CTCCTGTCAC CTTACCAGCA
    TTTGATGTTT CTATATTATT GGATATTTCA GACACCACAG TACTGAAACG TGTGGCTAGC
    TTGATGTCAG ATAAATCAAA GCCCTCTGAA ATTGAACAGG AGGACAGCAG TCAAAATTCA
    GATGTTAAAA TCGTAGAAGA GAAGACTGAC TTAGCAAGGG ACCAGGTGCT GCATAGACTT
    TCAGGCTTCC TAGACACATG GCCAAAATTA GAGAACTGGT TTTCAGACCA TCAGAATACT
    CTAGTGAAGG TCAATGCAGA GATGGAAGAA AGTACTGTGT GCAAAACTGT AAAGGAAATT
    CTGATTGAAG AAATTTACAA GAAGCAGAAT AGAAGGTATG TACAAGAAAA ATCACCAGAA
    GAAAAGGCTT TGCCAGTTAC ACCTCAAGAT CTGCTTTCCC CTGTTTCTGT GACACCACTG
    ATTAAGCCAG GAACAGATGA ATGGATTTAT GTAGATGAAC CACTTCCAAA GGAGATACCT
    GATTTTTTAG TACCTTATTG GGAAATGGTA GAAAATGTGT ATGTGAATAC CATCAAAACT
    GTACTTAGAT GTCTACGGGA CGAACAGTAC TCTGTGATAT ATTATTTAGC AGATATTAGA
    AAAAATTTCC AAGATTATTT AAAGCATCCA GATCATAAGC AGGAGTTTGT ATCTCAGTGG
    CAGTCAGATT TTAATTCAAT TCCTGATGAC CTGCGGGAAG ATGAGGAAAC AAAAGCAGAA
    CTACACCAAA GAGTAACTGA CTTAAGAGAT CTTCTCTGGG ATATCTGTGA TAAAAGAAGG
    GAAGAAGCAG AGCAAGAACG TACTGATATC ATGAATGATG GCTGGTTGCG AGATCATAAA
    GGGATTGCAA TAAACCATTT TTTTTCACTT ATGCAGGTTG AAGTGGATCG TTTCCAAGAT
    ACAAAGAGAC TTCTTCATGA CTATTATAGA GCAATGGAAG GAAAAATCCC AACAGAGGAC
    AGACAAAGTT TTACTAGACT TCCATTGCTA GATATTATTG ATAAAGAGCA GCAAGAAGAT
    CAGAATGAAT CAAGAAGGAT TCCTCTAGTT TCTTATAAAC TGCCTTCACC AGAAACAAAT
    ATTACTAAGT CAAAAAGCAA GGGAAATCTA CTGAAGAGTG TTAAGGATGA GAATTCTTCT
    GAAAATGTAG TGGTCGCTCT TGGGAAAGAT GAAAATGTTA TTCGTGATAC GTGGCAAACA
    GCAGTAACAG CAGTATCAGA TATGGTAAGA GCAGAAATAC AGTCAAAAGA GGTGAAGGAA
    GAAGGAGAAA AGCAGACAGA AGTGAAAGAA GATACAAAGT CTTCTCAAAC AATATCAAGC
    AAATCTACTG GGAAGGATGC CAAAGACACC AAAAAATCTC CCAAATCACC TACTAAAACG
    AAAGGACCTC GCTCCACTGC ATCTGCAGCT GAAGACAGTC CAGTTCCGTT AACCGCTGAA
    GAACTGAAGA AACAAAAACT GATACTTAAA ATACAGCAAG AATATTTTGC TGCCCTGAAA
    CATGAAGAGA AAGCTACAAA AACTCGACTC AAACTTATAA AAGCAAAAGC TTTAGCATAT
    GTTGAAGAGT TGACAATGAA AGCAGAAGAA GCTTATAAAG ATATGGAAAA GTGGCTTGGA
    GAAAGGTTCC TAGCAGAAAT GTCCAGTGTT GAAAAGCTGA TTGAGGTAGG ACGACACCAT
    ATTGAAAGCT CTAGCAAAAT CCAGTATGAA ATGAATTTAG AAGAAACAGA TTTCTTCATC
    AGCAGTGATG TAAAAGTGAT TCCTGATCCT GTTCCTCCAC CACAAGTTCC ACTAATAGAG
    ACTTCTGAAA GTAGTACTCT AACTATTTCG CAACTTAACA CATTTCATAA ACATTTTCTA
    CAAGTGGCAC CTAAAGCCAT AAGAGCACTG AAGCAATTTA AAGGCAGGAG TAATTCTTCT
    AGCAAGAGAG AAGTTGATGA CAGAGGGAAG GAAAAGAAAG ATTTGGAACA TCCAAGAATA
    AGGAAGAATC GCCGGTTTCC AAAAATATGG CTAACAAAAT ACTCCTGGTT AAAATATGAT
    GACGAAAGGG GAATAATGTT CTGTGCATTA TGTCGCAAGC ATAATGTGGA CCTGGGGGAA
    AGCATTCATA ATTTTTGTTC TGGTACTGAT GACTTCAAAC TGGAATTTAT AAATACACAC
    CAGAACAGTG AAGCTCATGC CTGGGCTACC TGCATGGAGG CTGCCAGTAC TGCTTCACCA
    AATACAGTTT CTGCTGAGCA GACATTGAAG AGTATGAACT CCATAACAAT AGGAAGAGTA
    GAAAGTACTT TTAGAACATG CCATGCAATT GCTAAATCTG TTCAGCCCTT TACAGACCTG
    GACTGGATTT ATAAATCAGA TGATGTGAAA GGAACAGATA TTGGTTCTCT ATTCAGAAAT
    TATAAGTCAG CAAGAATGTT TAAACATTTT ATGGCTGAAG CAGAAAGAAG AGCTCTAAAA
    GAGGAACTGG AGAATTGTAA ATTCTTTTCT CTTATTAGTG ATGAATTCAC AGATAGTATA
    TCCAAATCAG CTGCAGTTGT CTATGTGCAC TTTGCAAATG AAGGAAAAGT TCACTGCCAG
    ATTGTTGGGG TTCAACCTGT TCAAAAAAAT GATGCTTCAA CTATAAAAAG TGCAATTGAG
    GAAACATTTC AAATAAATCT TCAGCTTAGC TTGGCAAGCC AAGACTGGTC AAGAAAATTA
    GTTGGATTTG GAAGCGAGGA GACAGATGTA ATGACTGGTC AAAATAATGG GGTAGCTAAT
    CTCATGAGGG AAATTCAACC TTGTGTGCAG TCTGTGCATT GTTTTGCTCA CCGCCTAAGG
    CTGGCATACA AGGGAGCACT GGAAAACATT CAGCTATACA GCTTAACCAA TGGCTTGAGA
    AGTATGTACT ACTTTTATCA TAACTCAACT CTAAATAAGA AGAATCTCAA AGCTATATAT
    GAAGCCATCA AGTTACATCC AGCTATTCCA TCTTGCACTG GGGGCTCCCA GTGGTTTTCT
    CGCCTACAGA CAGCTCTACA AATTCTCCTT AAAGGTCATC CTGCATTTGT TCTACATCTG
    GATAAGACTA AAAGAGATTC AAGGTCCTCA AATAGGCAGA AAGTCAAAGG ACTATTGTAT
    CTCCTTCTGA AAATGGAGAT AGTCAAGTTT TCCCATTTCT TGTTGGATGT CATCAATGTC
    CTCAGCATTC TGTTACATAT TACTGTGGAT CACAATTCCT CCATTGCAGA TATATTTGCA
    ACTATGCAGT CAACTGTGGA AACACTCCAA ATGTATCAAG CAAGAGCTGG TCCAATGGAA
    CGCTTAATGA AGGCCATTCA ACATTTTCAT GGTCACCAGC TTGTTGGAAA TGGAAATATT
    TCAGCAGTAC GAACCAAAGT ACCAAGTAAT GTGGTGAAGA GGTTAAGTGA TTGTTTCTGT
    GATGCTAATC AAGGTGTCTT GAAAGCAACT CTTATTGGAA GTTTTAATCT ATGGCCTGAC
    AAAATGAAAC AAGTTATAGT ATTTGGTGAA AAGGAAGTAT CTGTCTTGAG TAAACATTAT
    GAAGCCATAC TAGAAGCTGC TAGTGTGAAG ATCAGTGAAG TTGAAATTGA ATGGAGCATG
    TTGAAATTAG AGCTATATAA CAGATTTCAG AACATCCAGA CCTTGACATG GGATTCGGTG
    AATGCACATT ATTCAAAGAA GTATCCCAGT ATCCTGGCAT TGGTAGATCT GATCCTAACT
    CTGCCAGCAA GCTCAGCTGA AATAGACCAA GGCTCTGGAA AAGAAAGTCA TCATGATGCA
    TCTGCACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153022
    Clone ID Related Accession (Same CDS sequence) XM_025144816.1
    Accession Version XM_025144816.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACAAT CTCAGCAGGA GCGGAGGATT GCTGTTCAAC TCATGCATGT TCGACATGAG 
    AAAGAGGTGT TAAAACAAAA TAGAATTTTC AGGGAAAAGC AATATGAAGA AAGACGACTG
    AAAGAGTTCC AGGAAGCTCT TGACAGAGAG GCGGCTCTTG CAAAACAAGA AAAAATTGAT
    TATGAAGAAC AAAGTGTCAA GGAAAGAGAG CGTCATGAGA AAATTGCCAT TGAAAGAGCT
    CAAGCTCGTT ATAAAAAGCA TTATTCTGCA TGTTGGGAAG TGATTGATCA GATTGTTGAT
    TTGTCAACAA AAATAGGAGA GTATCGAGTA TTGACAAACA ACAAAGTTCC GTTAAAACTG
    ATCTGTGATT GGAAGGAATT ATTTTTTAAT GGAAACTCAA TATATGAAAA AGCCTCAATT
    AAAACTTTGC CAAGTGAACC AAGCCCAGAA CAACTTCTGG AGTTGAATAA AATGGATCTA
    TTAGATGAAA AAGATTATGA TGAATACAAG AATATGACAG GGGAATGGTG CCCAACTGAA
    GAGAACAGAG AAAATAAATC TCTTAATAAT AACATATTGG GTCATGTTCT TCATAGACTG
    ATGGAGATTT TCTATCCTTC AAAACCCAGA TGTACACTAC CTGTACTTCC TTCATTTCCC
    ATCAAGGGAT GTATTCTGGG AAAAGTTTTT AGTGGAAAAA CTACCTGTGT AAAGTTTCTT
    GAAAAAGTTT GCAATATTCA GGTACTGTCT GTTGATACTC TGGTTCAGGA GGCCATCCAA
    GCTTTTCTTA ATAATGAAAT GAAAACTGAA CACAGCTTGA TTTCACAGGA AGCAGAGAGT
    TCTGCAAAAC AATACAAGGT CCAGAGGAAC TTCTCAGAAA CATCAATGAA ATATCTAACA
    GACACATTTA CTAATGGAAC CAAAGGTAAT CATCCTTTCA AAAAGCACCC ATCTGATCAG
    GAAACTGTAT CAGACCACAA AGAGAATGAC ATATCCAAGC TATCTGTACG TGCTCAACTT
    GGTGCTGCAT CTCAGAAATT GTTGAAGAAA GGAAAAAGTA TTCCTGATGA ATTACTAGTT
    GACATCCTAT TGGAAGCCAT TAAGCAAACA CCACCAGAGA AAGGATGGAT TCTGGATGGA
    TTTCCAACGA CAATTAATCA GGCAAGGCTA TTTGAAAAAG CCTGTACAGG GATTGATCCA
    GATGAAACAG AAGCAAAATA TGTGAACTGT GGAAAGCTCT CACTTGTAAC AAATCCTAGA
    ACACCTAGCA AGCCTCCTGT CACCTTACCA GCATTTGATG TTTCTATATT ATTGGATATT
    TCAGACACCA CAGTACTGAA ACGTGTGGCT AGCTTGATGT CAGATAAATC AAAGCCCTCT
    GAAATTGAAC AGGAGGACAG CAGTCAAAAT TCAGATGTTA AAATCGTAGA AGAGAAGACT
    GACTTAGCAA GGGACCAGGT GCTGCATAGA CTTTCAGGCT TCCTAGACAC ATGGCCAAAA
    TTAGAGAACT GGTTTTCAGA CCATCAGAAT ACTCTAGTGA AGGTCAATGC AGAGATGGAA
    GAAAGTACTG TGTGCAAAAC TGTAAAGGAA ATTCTGATTG AAGAAATTTA CAAGAAGCAG
    AATAGAAGGT ATGTACAAGA AAAATCACCA GAAGAAAAGG CTTTGCCAGT TACACCTCAA
    GATCTGCTTT CCCCTGTTTC TGTGACACCA CTGATTAAGC CAGGAACAGA TGAATGGATT
    TATGTAGATG AACCACTTCC AAAGGAGATA CCTGATTTTT TAGTACCTTA TTGGGAAATG
    GTAGAAAATG TGTATGTGAA TACCATCAAA ACTGTACTTA GATGTCTACG GGACGAACAG
    TACTCTGTGA TATATTATTT AGCAGATATT AGAAAAAATT TCCAAGATTA TTTAAAGCAT
    CCAGATCATA AGCAGGAGTT TGTATCTCAG TGGCAGTCAG ATTTTAATTC AATTCCTGAT
    GACCTGCGGG AAGATGAGGA AACAAAAGCA GAACTACACC AAAGAGTAAC TGACTTAAGA
    GATCTTCTCT GGGATATCTG TGATAAAAGA AGGGAAGAAG CAGAGCAAGA ACGTACTGAT
    ATCATGAATG ATGGCTGGTT GCGAGATCAT AAAGGGATTG CAATAAACCA TTTTTTTTCA
    CTTATGCAGG TTGAAGTGGA TCGTTTCCAA GATACAAAGA GACTTCTTCA TGACTATTAT
    AGAGCAATGG AAGGAAAAAT CCCAACAGAG GACAGACAAA GTTTTACTAG ACTTCCATTG
    CTAGATATTA TTGATAAAGA GCAGCAAGAA GATCAGAATG AATCAAGAAG GATTCCTCTA
    GTTTCTTATA AACTGCCTTC ACCAGAAACA AATATTACTA AGTCAAAAAG CAAGGGAAAT
    CTACTGAAGA GTGTTAAGGA TGAGAATTCT TCTGAAAATG TAGTGGTCGC TCTTGGGAAA
    GATGAAAATG TTATTCGTGA TACGTGGCAA ACAGCAGTAA CAGCAGTATC AGATATGGTA
    AGAGCAGAAA TACAGTCAAA AGAGGTGAAG GAAGAAGGAG AAAAGCAGAC AGAAGTGAAA
    GAAGATACAA AGTCTTCTCA AACAATATCA AGCAAATCTA CTGGGAAGGA TGCCAAAGAC
    ACCAAAAAAT CTCCCAAATC ACCTACTAAA ACGAAAGGAC CTCGCTCCAC TGCATCTGCA
    GCTGAAGACA GTCCAGTTCC GTTAACCGCT GAAGAACTGA AGAAACAAAA ACTGATACTT
    AAAATACAGC AAGAATATTT TGCTGCCCTG AAACATGAAG AGAAAGCTAC AAAAACTCGA
    CTCAAACTTA TAAAAGCAAA AGCTTTAGCA TATGTTGAAG AGTTGACAAT GAAAGCAGAA
    GAAGCTTATA AAGATATGGA AAAGTGGCTT GGAGAAAGGT TCCTAGCAGA AATGTCCAGT
    GTTGAAAAGC TGATTGAGGT AGGACGACAC CATATTGAAA GCTCTAGCAA AATCCAGTAT
    GAAATGAATT TAGAAGAAAC AGATTTCTTC ATCAGCAGTG ATGTAAAAGT GATTCCTGAT
    CCTGTTCCTC CACCACAAGT TCCACTAATA GAGACTTCTG AAAGTAGTAC TCTAACTATT
    TCGCAACTTA ACACATTTCA TAAACATTTT CTACAAGTGG CACCTAAAGC CATAAGAGCA
    CTGAAGCAAT TTAAAGGCAG GAGTAATTCT TCTAGCAAGA GAGAAGTTGA TGACAGAGGG
    AAGGAAAAGA AAGATTTGGA ACATCCAAGA ATAAGGAAGA ATCGCCGGTT TCCAAAAATA
    TGGCTAACAA AATACTCCTG GTTAAAATAT GATGACGAAA GGGGAATAAT GTTCTGTGCA
    TTATGTCGCA AGCATAATGT GGACCTGGGG GAAAGCATTC ATAATTTTTG TTCTGGTACT
    GATGACTTCA AACTGGAATT TATAAATACA CACCAGAACA GTGAAGCTCA TGCCTGGGCT
    ACCTGCATGG AGGCTGCCAG TACTGCTTCA CCAAATACAG TTTCTGCTGA GCAGACATTG
    AAGAGTATGA ACTCCATAAC AATAGGAAGA GTAGAAAGTA CTTTTAGAAC ATGCCATGCA
    ATTGCTAAAT CTGTTCAGCC CTTTACAGAC CTGGACTGGA TTTATAAATC AGATGATGTG
    AAAGGAACAG ATATTGGTTC TCTATTCAGA AATTATAAGT CAGCAAGAAT GTTTAAACAT
    TTTATGGCTG AAGCAGAAAG AAGAGCTCTA AAAGAGGAAC TGGAGAATTG TAAATTCTTT
    TCTCTTATTA GTGATGAATT CACAGATAGT ATATCCAAAT CAGCTGCAGT TGTCTATGTG
    CACTTTGCAA ATGAAGGAAA AGTTCACTGC CAGATTGTTG GGGTTCAACC TGTTCAAAAA
    AATGATGCTT CAACTATAAA AAGTGCAATT GAGGAAACAT TTCAAATAAA TCTTCAGCTT
    AGCTTGGCAA GCCAAGACTG GTCAAGAAAA TTAGTTGGAT TTGGAAGCGA GGAGACAGAT
    GTAATGACTG GTCAAAATAA TGGGGTAGCT AATCTCATGA GGGAAATTCA ACCTTGTGTG
    CAGTCTGTGC ATTGTTTTGC TCACCGCCTA AGGCTGGCAT ACAAGGGAGC ACTGGAAAAC
    ATTCAGCTAT ACAGCTTAAC CAATGGCTTG AGAAGTATGT ACTACTTTTA TCATAACTCA
    ACTCTAAATA AGAAGAATCT CAAAGCTATA TATGAAGCCA TCAAGTTACA TCCAGCTATT
    CCATCTTGCA CTGGGGGCTC CCAGTGGTTT TCTCGCCTAC AGACAGCTCT ACAAATTCTC
    CTTAAAGGTC ATCCTGCATT TGTTCTACAT CTGGATAAGA CTAAAAGAGA TTCAAGGTCC
    TCAAATAGGC AGAAAGTCAA AGGACTATTG TATCTCCTTC TGAAAATGGA GATAGTCAAG
    TTTTCCCATT TCTTGTTGGA TGTCATCAAT GTCCTCAGCA TTCTGTTACA TATTACTGTG
    GATCACAATT CCTCCATTGC AGATATATTT GCAACTATGC AGTCAACTGT GGAAACACTC
    CAAATGTATC AAGCAAGAGC TGGTCCAATG GAACGCTTAA TGAAGGCCAT TCAACATTTT
    CATGGTCACC AGCTTGTTGG AAATGGAAAT ATTTCAGCAG TACGAACCAA AGTACCAAGT
    AATGTGGTGA AGAGGTTAAG TGATTGTTTC TGTGATGCTA ATCAAGGTGT CTTGAAAGCA
    ACTCTTATTG GAAGTTTTAA TCTATGGCCT GACAAAATGA AACAAGTTAT AGTATTTGGT
    GAAAAGGAAG TATCTGTCTT GAGTAAACAT TATGAAGCCA TACTAGAAGC TGCTAGTGTG
    AAGATCAGTG AAGTTGAAAT TGAATGGAGC ATGTTGAAAT TAGAGCTATA TAACAGATTT
    CAGAACATCC AGACCTTGAC ATGGGATTCG GTGAATGCAC ATTATTCAAA GAAGTATCCC
    AGTATCCTGG CATTGGTAGA TCTGATCCTA ACTCTGCCAG CAAGCTCAGC TGAAATAGAC
    CAAGGCTCTG GAAAAGAAAG TCATCATGAT GCATCTGCAC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000584.1
    CDS922..6006
    Translation

    Target ORF information:

    RefSeq Version XM_025144816.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144816.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACAAT CTCAGCAGGA GCGGAGGATT GCTGTTCAAC TCATGCATGT TCGACATGAG 
    AAAGAGGTGT TAAAACAAAA TAGAATTTTC AGGGAAAAGC AATATGAAGA AAGACGACTG
    AAAGAGTTCC AGGAAGCTCT TGACAGAGAG GCGGCTCTTG CAAAACAAGA AAAAATTGAT
    TATGAAGAAC AAAGTGTCAA GGAAAGAGAG CGTCATGAGA AAATTGCCAT TGAAAGAGCT
    CAAGCTCGTT ATAAAAAGCA TTATTCTGCA TGTTGGGAAG TGATTGATCA GATTGTTGAT
    TTGTCAACAA AAATAGGAGA GTATCGAGTA TTGACAAACA ACAAAGTTCC GTTAAAACTG
    ATCTGTGATT GGAAGGAATT ATTTTTTAAT GGAAACTCAA TATATGAAAA AGCCTCAATT
    AAAACTTTGC CAAGTGAACC AAGCCCAGAA CAACTTCTGG AGTTGAATAA AATGGATCTA
    TTAGATGAAA AAGATTATGA TGAATACAAG AATATGACAG GGGAATGGTG CCCAACTGAA
    GAGAACAGAG AAAATAAATC TCTTAATAAT AACATATTGG GTCATGTTCT TCATAGACTG
    ATGGAGATTT TCTATCCTTC AAAACCCAGA TGTACACTAC CTGTACTTCC TTCATTTCCC
    ATCAAGGGAT GTATTCTGGG AAAAGTTTTT AGTGGAAAAA CTACCTGTGT AAAGTTTCTT
    GAAAAAGTTT GCAATATTCA GGTACTGTCT GTTGATACTC TGGTTCAGGA GGCCATCCAA
    GCTTTTCTTA ATAATGAAAT GAAAACTGAA CACAGCTTGA TTTCACAGGA AGCAGAGAGT
    TCTGCAAAAC AATACAAGGT CCAGAGGAAC TTCTCAGAAA CATCAATGAA ATATCTAACA
    GACACATTTA CTAATGGAAC CAAAGGTAAT CATCCTTTCA AAAAGCACCC ATCTGATCAG
    GAAACTGTAT CAGACCACAA AGAGAATGAC ATATCCAAGC TATCTGTACG TGCTCAACTT
    GGTGCTGCAT CTCAGAAATT GTTGAAGAAA GGAAAAAGTA TTCCTGATGA ATTACTAGTT
    GACATCCTAT TGGAAGCCAT TAAGCAAACA CCACCAGAGA AAGGATGGAT TCTGGATGGA
    TTTCCAACGA CAATTAATCA GGCAAGGCTA TTTGAAAAAG CCTGTACAGG GATTGATCCA
    GATGAAACAG AAGCAAAATA TGTGAACTGT GGAAAGCTCT CACTTGTAAC AAATCCTAGA
    ACACCTAGCA AGCCTCCTGT CACCTTACCA GCATTTGATG TTTCTATATT ATTGGATATT
    TCAGACACCA CAGTACTGAA ACGTGTGGCT AGCTTGATGT CAGATAAATC AAAGCCCTCT
    GAAATTGAAC AGGAGGACAG CAGTCAAAAT TCAGATGTTA AAATCGTAGA AGAGAAGACT
    GACTTAGCAA GGGACCAGGT GCTGCATAGA CTTTCAGGCT TCCTAGACAC ATGGCCAAAA
    TTAGAGAACT GGTTTTCAGA CCATCAGAAT ACTCTAGTGA AGGTCAATGC AGAGATGGAA
    GAAAGTACTG TGTGCAAAAC TGTAAAGGAA ATTCTGATTG AAGAAATTTA CAAGAAGCAG
    AATAGAAGGT ATGTACAAGA AAAATCACCA GAAGAAAAGG CTTTGCCAGT TACACCTCAA
    GATCTGCTTT CCCCTGTTTC TGTGACACCA CTGATTAAGC CAGGAACAGA TGAATGGATT
    TATGTAGATG AACCACTTCC AAAGGAGATA CCTGATTTTT TAGTACCTTA TTGGGAAATG
    GTAGAAAATG TGTATGTGAA TACCATCAAA ACTGTACTTA GATGTCTACG GGACGAACAG
    TACTCTGTGA TATATTATTT AGCAGATATT AGAAAAAATT TCCAAGATTA TTTAAAGCAT
    CCAGATCATA AGCAGGAGTT TGTATCTCAG TGGCAGTCAG ATTTTAATTC AATTCCTGAT
    GACCTGCGGG AAGATGAGGA AACAAAAGCA GAACTACACC AAAGAGTAAC TGACTTAAGA
    GATCTTCTCT GGGATATCTG TGATAAAAGA AGGGAAGAAG CAGAGCAAGA ACGTACTGAT
    ATCATGAATG ATGGCTGGTT GCGAGATCAT AAAGGGATTG CAATAAACCA TTTTTTTTCA
    CTTATGCAGG TTGAAGTGGA TCGTTTCCAA GATACAAAGA GACTTCTTCA TGACTATTAT
    AGAGCAATGG AAGGAAAAAT CCCAACAGAG GACAGACAAA GTTTTACTAG ACTTCCATTG
    CTAGATATTA TTGATAAAGA GCAGCAAGAA GATCAGAATG AATCAAGAAG GATTCCTCTA
    GTTTCTTATA AACTGCCTTC ACCAGAAACA AATATTACTA AGTCAAAAAG CAAGGGAAAT
    CTACTGAAGA GTGTTAAGGA TGAGAATTCT TCTGAAAATG TAGTGGTCGC TCTTGGGAAA
    GATGAAAATG TTATTCGTGA TACGTGGCAA ACAGCAGTAA CAGCAGTATC AGATATGGTA
    AGAGCAGAAA TACAGTCAAA AGAGGTGAAG GAAGAAGGAG AAAAGCAGAC AGAAGTGAAA
    GAAGATACAA AGTCTTCTCA AACAATATCA AGCAAATCTA CTGGGAAGGA TGCCAAAGAC
    ACCAAAAAAT CTCCCAAATC ACCTACTAAA ACGAAAGGAC CTCGCTCCAC TGCATCTGCA
    GCTGAAGACA GTCCAGTTCC GTTAACCGCT GAAGAACTGA AGAAACAAAA ACTGATACTT
    AAAATACAGC AAGAATATTT TGCTGCCCTG AAACATGAAG AGAAAGCTAC AAAAACTCGA
    CTCAAACTTA TAAAAGCAAA AGCTTTAGCA TATGTTGAAG AGTTGACAAT GAAAGCAGAA
    GAAGCTTATA AAGATATGGA AAAGTGGCTT GGAGAAAGGT TCCTAGCAGA AATGTCCAGT
    GTTGAAAAGC TGATTGAGGT AGGACGACAC CATATTGAAA GCTCTAGCAA AATCCAGTAT
    GAAATGAATT TAGAAGAAAC AGATTTCTTC ATCAGCAGTG ATGTAAAAGT GATTCCTGAT
    CCTGTTCCTC CACCACAAGT TCCACTAATA GAGACTTCTG AAAGTAGTAC TCTAACTATT
    TCGCAACTTA ACACATTTCA TAAACATTTT CTACAAGTGG CACCTAAAGC CATAAGAGCA
    CTGAAGCAAT TTAAAGGCAG GAGTAATTCT TCTAGCAAGA GAGAAGTTGA TGACAGAGGG
    AAGGAAAAGA AAGATTTGGA ACATCCAAGA ATAAGGAAGA ATCGCCGGTT TCCAAAAATA
    TGGCTAACAA AATACTCCTG GTTAAAATAT GATGACGAAA GGGGAATAAT GTTCTGTGCA
    TTATGTCGCA AGCATAATGT GGACCTGGGG GAAAGCATTC ATAATTTTTG TTCTGGTACT
    GATGACTTCA AACTGGAATT TATAAATACA CACCAGAACA GTGAAGCTCA TGCCTGGGCT
    ACCTGCATGG AGGCTGCCAG TACTGCTTCA CCAAATACAG TTTCTGCTGA GCAGACATTG
    AAGAGTATGA ACTCCATAAC AATAGGAAGA GTAGAAAGTA CTTTTAGAAC ATGCCATGCA
    ATTGCTAAAT CTGTTCAGCC CTTTACAGAC CTGGACTGGA TTTATAAATC AGATGATGTG
    AAAGGAACAG ATATTGGTTC TCTATTCAGA AATTATAAGT CAGCAAGAAT GTTTAAACAT
    TTTATGGCTG AAGCAGAAAG AAGAGCTCTA AAAGAGGAAC TGGAGAATTG TAAATTCTTT
    TCTCTTATTA GTGATGAATT CACAGATAGT ATATCCAAAT CAGCTGCAGT TGTCTATGTG
    CACTTTGCAA ATGAAGGAAA AGTTCACTGC CAGATTGTTG GGGTTCAACC TGTTCAAAAA
    AATGATGCTT CAACTATAAA AAGTGCAATT GAGGAAACAT TTCAAATAAA TCTTCAGCTT
    AGCTTGGCAA GCCAAGACTG GTCAAGAAAA TTAGTTGGAT TTGGAAGCGA GGAGACAGAT
    GTAATGACTG GTCAAAATAA TGGGGTAGCT AATCTCATGA GGGAAATTCA ACCTTGTGTG
    CAGTCTGTGC ATTGTTTTGC TCACCGCCTA AGGCTGGCAT ACAAGGGAGC ACTGGAAAAC
    ATTCAGCTAT ACAGCTTAAC CAATGGCTTG AGAAGTATGT ACTACTTTTA TCATAACTCA
    ACTCTAAATA AGAAGAATCT CAAAGCTATA TATGAAGCCA TCAAGTTACA TCCAGCTATT
    CCATCTTGCA CTGGGGGCTC CCAGTGGTTT TCTCGCCTAC AGACAGCTCT ACAAATTCTC
    CTTAAAGGTC ATCCTGCATT TGTTCTACAT CTGGATAAGA CTAAAAGAGA TTCAAGGTCC
    TCAAATAGGC AGAAAGTCAA AGGACTATTG TATCTCCTTC TGAAAATGGA GATAGTCAAG
    TTTTCCCATT TCTTGTTGGA TGTCATCAAT GTCCTCAGCA TTCTGTTACA TATTACTGTG
    GATCACAATT CCTCCATTGC AGATATATTT GCAACTATGC AGTCAACTGT GGAAACACTC
    CAAATGTATC AAGCAAGAGC TGGTCCAATG GAACGCTTAA TGAAGGCCAT TCAACATTTT
    CATGGTCACC AGCTTGTTGG AAATGGAAAT ATTTCAGCAG TACGAACCAA AGTACCAAGT
    AATGTGGTGA AGAGGTTAAG TGATTGTTTC TGTGATGCTA ATCAAGGTGT CTTGAAAGCA
    ACTCTTATTG GAAGTTTTAA TCTATGGCCT GACAAAATGA AACAAGTTAT AGTATTTGGT
    GAAAAGGAAG TATCTGTCTT GAGTAAACAT TATGAAGCCA TACTAGAAGC TGCTAGTGTG
    AAGATCAGTG AAGTTGAAAT TGAATGGAGC ATGTTGAAAT TAGAGCTATA TAACAGATTT
    CAGAACATCC AGACCTTGAC ATGGGATTCG GTGAATGCAC ATTATTCAAA GAAGTATCCC
    AGTATCCTGG CATTGGTAGA TCTGATCCTA ACTCTGCCAG CAAGCTCAGC TGAAATAGAC
    CAAGGCTCTG GAAAAGAAAG TCATCATGAT GCATCTGCAC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153018
    Clone ID Related Accession (Same CDS sequence) XM_025144811.1
    Accession Version XM_025144811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTATAA ATCAAAGCCC TCTGAAATTG AACAGGAGGA CAGCAGTCAA
    AATTCAGATG TTAAAATCGT AGAAGAGAAG ACTGACTTAG CAAGGGACCA GGTGCTGCAT
    AGACTTTCAG GCTTCCTAGA CACATGGCCA AAATTAGAGA ACTGGTTTTC AGACCATCAG
    AATACTCTAG TGAAGGTCAA TGCAGAGATG GAAGAAAGTA CTGTGTGCAA AACTGTAAAG
    GAAATTCTGA TTGAAGAAAT TTACAAGAAG CAGAATAGAA GGTATGTACA AGAAAAATCA
    CCAGAAGAAA AGGCTTTGCC AGTTACACCT CAAGATCTGC TTTCCCCTGT TTCTGTGACA
    CCACTGATTA AGCCAGGAAC AGATGAATGG ATTTATGTAG ATGAACCACT TCCAAAGGAG
    ATACCTGATT TTTTAGTACC TTATTGGGAA ATGGTAGAAA ATGTGTATGT GAATACCATC
    AAAACTGTAC TTAGATGTCT ACGGGACGAA CAGTACTCTG TGATATATTA TTTAGCAGAT
    ATTAGAAAAA ATTTCCAAGA TTATTTAAAG CATCCAGATC ATAAGCAGGA GTTTGTATCT
    CAGTGGCAGT CAGATTTTAA TTCAATTCCT GATGACCTGC GGGAAGATGA GGAAACAAAA
    GCAGAACTAC ACCAAAGAGT AACTGACTTA AGAGATCTTC TCTGGGATAT CTGTGATAAA
    AGAAGGGAAG AAGCAGAGCA AGAACGTACT GATATCATGA ATGATGGCTG GTTGCGAGAT
    CATAAAGGGA TTGCAATAAA CCATTTTTTT TCACTTATGC AGGTTGAAGT GGATCGTTTC
    CAAGATACAA AGAGACTTCT TCATGACTAT TATAGAGCAA TGGAAGGAAA AATCCCAACA
    GAGGACAGAC AAAGTTTTAC TAGACTTCCA TTGCTAGATA TTATTGATAA AGAGCAGCAA
    GAAGATCAGA ATGAATCAAG AAGGATTCCT CTAGTTTCTT ATAAACTGCC TTCACCAGAA
    ACAAATATTA CTAAGTCAAA AAGCAAGGGA AATCTACTGA AGAGTGTTAA GGATGAGAAT
    TCTTCTGAAA ATGTAGTGGT CGCTCTTGGG AAAGATGAAA ATGTTATTCG TGATACGTGG
    CAAACAGCAG TAACAGCAGT ATCAGATATG GTAAGAGCAG AAATACAGTC AAAAGAGGTG
    AAGGAAGAAG GAGAAAAGCA GACAGAAGTG AAAGAAGATA CAAAGTCTTC TCAAACAATA
    TCAAGCAAAT CTACTGGGAA GGATGCCAAA GACACCAAAA AATCTCCCAA ATCACCTACT
    AAAACGAAAG GACCTCGCTC CACTGCATCT GCAGCTGAAG ACAGTCCAGT TCCGTTAACC
    GCTGAAGAAC TGAAGAAACA AAAACTGATA CTTAAAATAC AGCAAGAATA TTTTGCTGCC
    CTGAAACATG AAGAGAAAGC TACAAAAACT CGACTCAAAC TTATAAAAGC AAAAGCTTTA
    GCATATGTTG AAGAGTTGAC AATGAAAGCA GAAGAAGCTT ATAAAGATAT GGAAAAGTGG
    CTTGGAGAAA GGTTCCTAGC AGAAATGTCC AGTGTTGAAA AGCTGATTGA GGTAGGACGA
    CACCATATTG AAAGCTCTAG CAAAATCCAG TATGAAATGA ATTTAGAAGA AACAGATTTC
    TTCATCAGCA GTGATGTAAA AGTGATTCCT GATCCTGTTC CTCCACCACA AGTTCCACTA
    ATAGAGACTT CTGAAAGTAG TACTCTAACT ATTTCGCAAC TTAACACATT TCATAAACAT
    TTTCTACAAG TGGCACCTAA AGCCATAAGA GCACTGAAGC AATTTAAAGG CAGGAGTAAT
    TCTTCTAGCA AGAGAGAAGT TGATGACAGA GGGAAGGAAA AGAAAGATTT GGAACATCCA
    AGAATAAGGA AGAATCGCCG GTTTCCAAAA ATATGGCTAA CAAAATACTC CTGGTTAAAA
    TATGATGACG AAAGGGGAAT AATGTTCTGT GCATTATGTC GCAAGCATAA TGTGGACCTG
    GGGGAAAGCA TTCATAATTT TTGTTCTGGT ACTGATGACT TCAAACTGGA ATTTATAAAT
    ACACACCAGA ACAGTGAAGC TCATGCCTGG GCTACCTGCA TGGAGGCTGC CAGTACTGCT
    TCACCAAATA CAGTTTCTGC TGAGCAGACA TTGAAGAGTA TGAACTCCAT AACAATAGGA
    AGAGTAGAAA GTACTTTTAG AACATGCCAT GCAATTGCTA AATCTGTTCA GCCCTTTACA
    GACCTGGACT GGATTTATAA ATCAGATGAT GTGAAAGGAA CAGATATTGG TTCTCTATTC
    AGAAATTATA AGTCAGCAAG AATGTTTAAA CATTTTATGG CTGAAGCAGA AAGAAGAGCT
    CTAAAAGAGG AACTGGAGAA TTGTAAATTC TTTTCTCTTA TTAGTGATGA ATTCACAGAT
    AGTATATCCA AATCAGCTGC AGTTGTCTAT GTGCACTTTG CAAATGAAGG AAAAGTTCAC
    TGCCAGATTG TTGGGGTTCA ACCTGTTCAA AAAAATGATG CTTCAACTAT AAAAAGTGCA
    ATTGAGGAAA CATTTCAAAT AAATCTTCAG CTTAGCTTGG CAAGCCAAGA CTGGTCAAGA
    AAATTAGTTG GATTTGGAAG CGAGGAGACA GATGTAATGA CTGGTCAAAA TAATGGGGTA
    GCTAATCTCA TGAGGGAAAT TCAACCTTGT GTGCAGTCTG TGCATTGTTT TGCTCACCGC
    CTAAGGCTGG CATACAAGGG AGCACTGGAA AACATTCAGC TATACAGCTT AACCAATGGC
    TTGAGAAGTA TGTACTACTT TTATCATAAC TCAACTCTAA ATAAGAAGAA TCTCAAAGCT
    ATATATGAAG CCATCAAGTT ACATCCAGCT ATTCCATCTT GCACTGGGGG CTCCCAGTGG
    TTTTCTCGCC TACAGACAGC TCTACAAATT CTCCTTAAAG GTCATCCTGC ATTTGTTCTA
    CATCTGGATA AGACTAAAAG AGATTCAAGG TCCTCAAATA GGCAGAAAGT CAAAGGACTA
    TTGTATCTCC TTCTGAAAAT GGAGATAGTC AAGTTTTCCC ATTTCTTGTT GGATGTCATC
    AATGTCCTCA GCATTCTGTT ACATATTACT GTGGATCACA ATTCCTCCAT TGCAGATATA
    TTTGCAACTA TGCAGTCAAC TGTGGAAACA CTCCAAATGT ATCAAGCAAG AGCTGGTCCA
    ATGGAACGCT TAATGAAGGC CATTCAACAT TTTCATGGTC ACCAGCTTGT TGGAAATGGA
    AATATTTCAG CAGTACGAAC CAAAGTACCA AGTAATGTGG TGAAGAGGTT AAGTGATTGT
    TTCTGTGATG CTAATCAAGG TGTCTTGAAA GCAACTCTTA TTGGAAGTTT TAATCTATGG
    CCTGACAAAA TGAAACAAGT TATAGTATTT GGTGAAAAGG AAGTATCTGT CTTGAGTAAA
    CATTATGAAG CCATACTAGA AGCTGCTAGT GTGAAGATCA GTGAAGTTGA AATTGAATGG
    AGCATGTTGA AATTAGAGCT ATATAACAGA TTTCAGAACA TCCAGACCTT GACATGGGAT
    TCGGTGAATG CACATTATTC AAAGAAGTAT CCCAGTATCC TGGCATTGGT AGATCTGATC
    CTAACTCTGC CAGCAAGCTC AGCTGAAATA GACCAAGGCT CTGGAAAAGA AAGTCATCAT
    GATGCATCTG CACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000579.1
    CDS35..6172
    Translation

    Target ORF information:

    RefSeq Version XM_025144811.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTATAA ATCAAAGCCC TCTGAAATTG AACAGGAGGA CAGCAGTCAA
    AATTCAGATG TTAAAATCGT AGAAGAGAAG ACTGACTTAG CAAGGGACCA GGTGCTGCAT
    AGACTTTCAG GCTTCCTAGA CACATGGCCA AAATTAGAGA ACTGGTTTTC AGACCATCAG
    AATACTCTAG TGAAGGTCAA TGCAGAGATG GAAGAAAGTA CTGTGTGCAA AACTGTAAAG
    GAAATTCTGA TTGAAGAAAT TTACAAGAAG CAGAATAGAA GGTATGTACA AGAAAAATCA
    CCAGAAGAAA AGGCTTTGCC AGTTACACCT CAAGATCTGC TTTCCCCTGT TTCTGTGACA
    CCACTGATTA AGCCAGGAAC AGATGAATGG ATTTATGTAG ATGAACCACT TCCAAAGGAG
    ATACCTGATT TTTTAGTACC TTATTGGGAA ATGGTAGAAA ATGTGTATGT GAATACCATC
    AAAACTGTAC TTAGATGTCT ACGGGACGAA CAGTACTCTG TGATATATTA TTTAGCAGAT
    ATTAGAAAAA ATTTCCAAGA TTATTTAAAG CATCCAGATC ATAAGCAGGA GTTTGTATCT
    CAGTGGCAGT CAGATTTTAA TTCAATTCCT GATGACCTGC GGGAAGATGA GGAAACAAAA
    GCAGAACTAC ACCAAAGAGT AACTGACTTA AGAGATCTTC TCTGGGATAT CTGTGATAAA
    AGAAGGGAAG AAGCAGAGCA AGAACGTACT GATATCATGA ATGATGGCTG GTTGCGAGAT
    CATAAAGGGA TTGCAATAAA CCATTTTTTT TCACTTATGC AGGTTGAAGT GGATCGTTTC
    CAAGATACAA AGAGACTTCT TCATGACTAT TATAGAGCAA TGGAAGGAAA AATCCCAACA
    GAGGACAGAC AAAGTTTTAC TAGACTTCCA TTGCTAGATA TTATTGATAA AGAGCAGCAA
    GAAGATCAGA ATGAATCAAG AAGGATTCCT CTAGTTTCTT ATAAACTGCC TTCACCAGAA
    ACAAATATTA CTAAGTCAAA AAGCAAGGGA AATCTACTGA AGAGTGTTAA GGATGAGAAT
    TCTTCTGAAA ATGTAGTGGT CGCTCTTGGG AAAGATGAAA ATGTTATTCG TGATACGTGG
    CAAACAGCAG TAACAGCAGT ATCAGATATG GTAAGAGCAG AAATACAGTC AAAAGAGGTG
    AAGGAAGAAG GAGAAAAGCA GACAGAAGTG AAAGAAGATA CAAAGTCTTC TCAAACAATA
    TCAAGCAAAT CTACTGGGAA GGATGCCAAA GACACCAAAA AATCTCCCAA ATCACCTACT
    AAAACGAAAG GACCTCGCTC CACTGCATCT GCAGCTGAAG ACAGTCCAGT TCCGTTAACC
    GCTGAAGAAC TGAAGAAACA AAAACTGATA CTTAAAATAC AGCAAGAATA TTTTGCTGCC
    CTGAAACATG AAGAGAAAGC TACAAAAACT CGACTCAAAC TTATAAAAGC AAAAGCTTTA
    GCATATGTTG AAGAGTTGAC AATGAAAGCA GAAGAAGCTT ATAAAGATAT GGAAAAGTGG
    CTTGGAGAAA GGTTCCTAGC AGAAATGTCC AGTGTTGAAA AGCTGATTGA GGTAGGACGA
    CACCATATTG AAAGCTCTAG CAAAATCCAG TATGAAATGA ATTTAGAAGA AACAGATTTC
    TTCATCAGCA GTGATGTAAA AGTGATTCCT GATCCTGTTC CTCCACCACA AGTTCCACTA
    ATAGAGACTT CTGAAAGTAG TACTCTAACT ATTTCGCAAC TTAACACATT TCATAAACAT
    TTTCTACAAG TGGCACCTAA AGCCATAAGA GCACTGAAGC AATTTAAAGG CAGGAGTAAT
    TCTTCTAGCA AGAGAGAAGT TGATGACAGA GGGAAGGAAA AGAAAGATTT GGAACATCCA
    AGAATAAGGA AGAATCGCCG GTTTCCAAAA ATATGGCTAA CAAAATACTC CTGGTTAAAA
    TATGATGACG AAAGGGGAAT AATGTTCTGT GCATTATGTC GCAAGCATAA TGTGGACCTG
    GGGGAAAGCA TTCATAATTT TTGTTCTGGT ACTGATGACT TCAAACTGGA ATTTATAAAT
    ACACACCAGA ACAGTGAAGC TCATGCCTGG GCTACCTGCA TGGAGGCTGC CAGTACTGCT
    TCACCAAATA CAGTTTCTGC TGAGCAGACA TTGAAGAGTA TGAACTCCAT AACAATAGGA
    AGAGTAGAAA GTACTTTTAG AACATGCCAT GCAATTGCTA AATCTGTTCA GCCCTTTACA
    GACCTGGACT GGATTTATAA ATCAGATGAT GTGAAAGGAA CAGATATTGG TTCTCTATTC
    AGAAATTATA AGTCAGCAAG AATGTTTAAA CATTTTATGG CTGAAGCAGA AAGAAGAGCT
    CTAAAAGAGG AACTGGAGAA TTGTAAATTC TTTTCTCTTA TTAGTGATGA ATTCACAGAT
    AGTATATCCA AATCAGCTGC AGTTGTCTAT GTGCACTTTG CAAATGAAGG AAAAGTTCAC
    TGCCAGATTG TTGGGGTTCA ACCTGTTCAA AAAAATGATG CTTCAACTAT AAAAAGTGCA
    ATTGAGGAAA CATTTCAAAT AAATCTTCAG CTTAGCTTGG CAAGCCAAGA CTGGTCAAGA
    AAATTAGTTG GATTTGGAAG CGAGGAGACA GATGTAATGA CTGGTCAAAA TAATGGGGTA
    GCTAATCTCA TGAGGGAAAT TCAACCTTGT GTGCAGTCTG TGCATTGTTT TGCTCACCGC
    CTAAGGCTGG CATACAAGGG AGCACTGGAA AACATTCAGC TATACAGCTT AACCAATGGC
    TTGAGAAGTA TGTACTACTT TTATCATAAC TCAACTCTAA ATAAGAAGAA TCTCAAAGCT
    ATATATGAAG CCATCAAGTT ACATCCAGCT ATTCCATCTT GCACTGGGGG CTCCCAGTGG
    TTTTCTCGCC TACAGACAGC TCTACAAATT CTCCTTAAAG GTCATCCTGC ATTTGTTCTA
    CATCTGGATA AGACTAAAAG AGATTCAAGG TCCTCAAATA GGCAGAAAGT CAAAGGACTA
    TTGTATCTCC TTCTGAAAAT GGAGATAGTC AAGTTTTCCC ATTTCTTGTT GGATGTCATC
    AATGTCCTCA GCATTCTGTT ACATATTACT GTGGATCACA ATTCCTCCAT TGCAGATATA
    TTTGCAACTA TGCAGTCAAC TGTGGAAACA CTCCAAATGT ATCAAGCAAG AGCTGGTCCA
    ATGGAACGCT TAATGAAGGC CATTCAACAT TTTCATGGTC ACCAGCTTGT TGGAAATGGA
    AATATTTCAG CAGTACGAAC CAAAGTACCA AGTAATGTGG TGAAGAGGTT AAGTGATTGT
    TTCTGTGATG CTAATCAAGG TGTCTTGAAA GCAACTCTTA TTGGAAGTTT TAATCTATGG
    CCTGACAAAA TGAAACAAGT TATAGTATTT GGTGAAAAGG AAGTATCTGT CTTGAGTAAA
    CATTATGAAG CCATACTAGA AGCTGCTAGT GTGAAGATCA GTGAAGTTGA AATTGAATGG
    AGCATGTTGA AATTAGAGCT ATATAACAGA TTTCAGAACA TCCAGACCTT GACATGGGAT
    TCGGTGAATG CACATTATTC AAAGAAGTAT CCCAGTATCC TGGCATTGGT AGATCTGATC
    CTAACTCTGC CAGCAAGCTC AGCTGAAATA GACCAAGGCT CTGGAAAAGA AAGTCATCAT
    GATGCATCTG CACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47840
    Clone ID Related Accession (Same CDS sequence) XM_015277513.1 , XM_015277513.2
    Accession Version XM_015277513.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGCCATA AGAGCACTGA AGCAATTTAA AGGCAGGAGT
    AATTCTTCTA GCAAGAGAGA AGTTGATGAC AGAGGGAAGG AAAAGAAAGA TTTGGAACAT
    CCAAGAATAA GGAAGAATCG CCGGTTTCCA AAAATATGGC TAACAAAATA CTCCTGGTTA
    AAATATGATG ACGAAAGGGG AATAATGTTC TGTGCATTAT GTCGCAAGCA TAATGTGGAC
    CTGGGGGAAA GCATTCATAA TTTTTGTTCT GGTACTGATG ACTTCAAACT GGAATTTATA
    AATACACACC AGAACAGTGA AGCTCATGCC TGGGCTACCT GCATGGAGGC TGCCAGTACT
    GCTTCACCAA ATACAGTTTC TGCTGAGCAG ACATTGAAGA GTATGAACTC CATAACAATA
    GGAAGAGTAG AAAGTACTTT TAGAACATGC CATGCAATTG CTAAATCTGT TCAGCCCTTT
    ACAGACCTGG ACTGGATTTA TAAATCAGAT GATGTGAAAG GAACAGATAT TGGTTCTCTA
    TTCAGAAATT ATAAGTCAGC AAGAATGTTT AAACATTTTA TGGCTGAAGC AGAAAGAAGA
    GCTCTAAAAG AGGAACTGGA GAATTGTAAA TTCTTTTCTC TTATTAGTGA TGAATTCACA
    GATAGTATAT CCAAATCAGC TGCAGTTGTC TATGTGCACT TTGCAAATGA AGGAAAAGTT
    CACTGCCAGA TTGTTGGGGT TCAACCTGTT CAAAAAAATG ATGCTTCAAC TATAAAAAGT
    GCAATTGAGG AAACATTTCA AATAAATCTT CAGCTTAGCT TGGCAAGCCA AGACTGGTCA
    AGAAAATTAG TTGGATTTGG AAGCGAGGAG ACAGATGTAA TGACTGGTCA AAATAATGGG
    GTAGCTAATC TCATGAGGGA AATTCAACCT TGTGTGCAGT CTGTGCATTG TTTTGCTCAC
    CGCCTAAGGC TGGCATACAA GGGAGCACTG GAAAACATTC AGCTATACAG CTTAACCAAT
    GGCTTGAGAA GTATGTACTA CTTTTATCAT AACTCAACTC TAAATAAGAA GAATCTCAAA
    GCTATATATG AAGCCATCAA GTTACATCCA GCTATTCCAT CTTGCACTGG GGGCTCCCAG
    TGGTTTTCTC GCCTACAGAC AGCTCTACAA ATTCTCCTTA AAGGTCATCC TGCATTTGTT
    CTACATCTGG ATAAGACTAA AAGAGATTCA AGGTCCTCAA ATAGGCAGAA AGTCAAAGGA
    CTATTGTATC TCCTTCTGAA AATGGAGATA GTCAAGTTTT CCCATTTCTT GTTGGATGTC
    ATCAATGTCC TCAGCATTCT GTTACATATT ACTGTGGATC ACAATTCCTC CATTGCAGAT
    ATATTTGCAA CTATGCAGTC AACTGTGGAA ACACTCCAAA TGTATCAAGC AAGAGCTGGT
    CCAATGGAAC GCTTAATGAA GGCCATTCAA CATTTTCATG GTCACCAGCT TGTTGGAAAT
    GGAAATATTT CAGCAGTACG AACCAAAGTA CCAAGTAATG TGGTGAAGAG GTTAAGTGAT
    TGTTTCTGTG ATGCTAATCA AGGTGTCTTG AAAGCAACTC TTATTGGAAG TTTTAATCTA
    TGGCCTGACA AAATGAAACA AGTTATAGTA TTTGGTGAAA AGGAAGTATC TGTCTTGAGT
    AAACATTATG AAGCCATACT AGAAGCTGCT AGTGTGAAGA TCAGTGAAGT TGAAATTGAA
    TGGAGCATGT TGAAATTAGA GCTATATAAC AGATTTCAGA ACATCCAGAC CTTGACATGG
    GATTCGGTGA ATGCACATTA TTCAAAGAAG TATCCCAGTA TCCTGGCATT GGTAGATCTG
    ATCCTAACTC TGCCAGCAAG CTCAGCTGAA ATAGACCAAG GCTCTGGAAA AGAAAGTCAT
    CATGATGCAT CTGCACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132999.1
    CDS156..6296
    Translation

    Target ORF information:

    RefSeq Version XM_015277513.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGCCATA AGAGCACTGA AGCAATTTAA AGGCAGGAGT
    AATTCTTCTA GCAAGAGAGA AGTTGATGAC AGAGGGAAGG AAAAGAAAGA TTTGGAACAT
    CCAAGAATAA GGAAGAATCG CCGGTTTCCA AAAATATGGC TAACAAAATA CTCCTGGTTA
    AAATATGATG ACGAAAGGGG AATAATGTTC TGTGCATTAT GTCGCAAGCA TAATGTGGAC
    CTGGGGGAAA GCATTCATAA TTTTTGTTCT GGTACTGATG ACTTCAAACT GGAATTTATA
    AATACACACC AGAACAGTGA AGCTCATGCC TGGGCTACCT GCATGGAGGC TGCCAGTACT
    GCTTCACCAA ATACAGTTTC TGCTGAGCAG ACATTGAAGA GTATGAACTC CATAACAATA
    GGAAGAGTAG AAAGTACTTT TAGAACATGC CATGCAATTG CTAAATCTGT TCAGCCCTTT
    ACAGACCTGG ACTGGATTTA TAAATCAGAT GATGTGAAAG GAACAGATAT TGGTTCTCTA
    TTCAGAAATT ATAAGTCAGC AAGAATGTTT AAACATTTTA TGGCTGAAGC AGAAAGAAGA
    GCTCTAAAAG AGGAACTGGA GAATTGTAAA TTCTTTTCTC TTATTAGTGA TGAATTCACA
    GATAGTATAT CCAAATCAGC TGCAGTTGTC TATGTGCACT TTGCAAATGA AGGAAAAGTT
    CACTGCCAGA TTGTTGGGGT TCAACCTGTT CAAAAAAATG ATGCTTCAAC TATAAAAAGT
    GCAATTGAGG AAACATTTCA AATAAATCTT CAGCTTAGCT TGGCAAGCCA AGACTGGTCA
    AGAAAATTAG TTGGATTTGG AAGCGAGGAG ACAGATGTAA TGACTGGTCA AAATAATGGG
    GTAGCTAATC TCATGAGGGA AATTCAACCT TGTGTGCAGT CTGTGCATTG TTTTGCTCAC
    CGCCTAAGGC TGGCATACAA GGGAGCACTG GAAAACATTC AGCTATACAG CTTAACCAAT
    GGCTTGAGAA GTATGTACTA CTTTTATCAT AACTCAACTC TAAATAAGAA GAATCTCAAA
    GCTATATATG AAGCCATCAA GTTACATCCA GCTATTCCAT CTTGCACTGG GGGCTCCCAG
    TGGTTTTCTC GCCTACAGAC AGCTCTACAA ATTCTCCTTA AAGGTCATCC TGCATTTGTT
    CTACATCTGG ATAAGACTAA AAGAGATTCA AGGTCCTCAA ATAGGCAGAA AGTCAAAGGA
    CTATTGTATC TCCTTCTGAA AATGGAGATA GTCAAGTTTT CCCATTTCTT GTTGGATGTC
    ATCAATGTCC TCAGCATTCT GTTACATATT ACTGTGGATC ACAATTCCTC CATTGCAGAT
    ATATTTGCAA CTATGCAGTC AACTGTGGAA ACACTCCAAA TGTATCAAGC AAGAGCTGGT
    CCAATGGAAC GCTTAATGAA GGCCATTCAA CATTTTCATG GTCACCAGCT TGTTGGAAAT
    GGAAATATTT CAGCAGTACG AACCAAAGTA CCAAGTAATG TGGTGAAGAG GTTAAGTGAT
    TGTTTCTGTG ATGCTAATCA AGGTGTCTTG AAAGCAACTC TTATTGGAAG TTTTAATCTA
    TGGCCTGACA AAATGAAACA AGTTATAGTA TTTGGTGAAA AGGAAGTATC TGTCTTGAGT
    AAACATTATG AAGCCATACT AGAAGCTGCT AGTGTGAAGA TCAGTGAAGT TGAAATTGAA
    TGGAGCATGT TGAAATTAGA GCTATATAAC AGATTTCAGA ACATCCAGAC CTTGACATGG
    GATTCGGTGA ATGCACATTA TTCAAAGAAG TATCCCAGTA TCCTGGCATT GGTAGATCTG
    ATCCTAACTC TGCCAGCAAG CTCAGCTGAA ATAGACCAAG GCTCTGGAAA AGAAAGTCAT
    CATGATGCAT CTGCACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47840
    Clone ID Related Accession (Same CDS sequence) XM_015277513.1 , XM_015277513.2
    Accession Version XM_015277513.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGCCATA AGAGCACTGA AGCAATTTAA AGGCAGGAGT
    AATTCTTCTA GCAAGAGAGA AGTTGATGAC AGAGGGAAGG AAAAGAAAGA TTTGGAACAT
    CCAAGAATAA GGAAGAATCG CCGGTTTCCA AAAATATGGC TAACAAAATA CTCCTGGTTA
    AAATATGATG ACGAAAGGGG AATAATGTTC TGTGCATTAT GTCGCAAGCA TAATGTGGAC
    CTGGGGGAAA GCATTCATAA TTTTTGTTCT GGTACTGATG ACTTCAAACT GGAATTTATA
    AATACACACC AGAACAGTGA AGCTCATGCC TGGGCTACCT GCATGGAGGC TGCCAGTACT
    GCTTCACCAA ATACAGTTTC TGCTGAGCAG ACATTGAAGA GTATGAACTC CATAACAATA
    GGAAGAGTAG AAAGTACTTT TAGAACATGC CATGCAATTG CTAAATCTGT TCAGCCCTTT
    ACAGACCTGG ACTGGATTTA TAAATCAGAT GATGTGAAAG GAACAGATAT TGGTTCTCTA
    TTCAGAAATT ATAAGTCAGC AAGAATGTTT AAACATTTTA TGGCTGAAGC AGAAAGAAGA
    GCTCTAAAAG AGGAACTGGA GAATTGTAAA TTCTTTTCTC TTATTAGTGA TGAATTCACA
    GATAGTATAT CCAAATCAGC TGCAGTTGTC TATGTGCACT TTGCAAATGA AGGAAAAGTT
    CACTGCCAGA TTGTTGGGGT TCAACCTGTT CAAAAAAATG ATGCTTCAAC TATAAAAAGT
    GCAATTGAGG AAACATTTCA AATAAATCTT CAGCTTAGCT TGGCAAGCCA AGACTGGTCA
    AGAAAATTAG TTGGATTTGG AAGCGAGGAG ACAGATGTAA TGACTGGTCA AAATAATGGG
    GTAGCTAATC TCATGAGGGA AATTCAACCT TGTGTGCAGT CTGTGCATTG TTTTGCTCAC
    CGCCTAAGGC TGGCATACAA GGGAGCACTG GAAAACATTC AGCTATACAG CTTAACCAAT
    GGCTTGAGAA GTATGTACTA CTTTTATCAT AACTCAACTC TAAATAAGAA GAATCTCAAA
    GCTATATATG AAGCCATCAA GTTACATCCA GCTATTCCAT CTTGCACTGG GGGCTCCCAG
    TGGTTTTCTC GCCTACAGAC AGCTCTACAA ATTCTCCTTA AAGGTCATCC TGCATTTGTT
    CTACATCTGG ATAAGACTAA AAGAGATTCA AGGTCCTCAA ATAGGCAGAA AGTCAAAGGA
    CTATTGTATC TCCTTCTGAA AATGGAGATA GTCAAGTTTT CCCATTTCTT GTTGGATGTC
    ATCAATGTCC TCAGCATTCT GTTACATATT ACTGTGGATC ACAATTCCTC CATTGCAGAT
    ATATTTGCAA CTATGCAGTC AACTGTGGAA ACACTCCAAA TGTATCAAGC AAGAGCTGGT
    CCAATGGAAC GCTTAATGAA GGCCATTCAA CATTTTCATG GTCACCAGCT TGTTGGAAAT
    GGAAATATTT CAGCAGTACG AACCAAAGTA CCAAGTAATG TGGTGAAGAG GTTAAGTGAT
    TGTTTCTGTG ATGCTAATCA AGGTGTCTTG AAAGCAACTC TTATTGGAAG TTTTAATCTA
    TGGCCTGACA AAATGAAACA AGTTATAGTA TTTGGTGAAA AGGAAGTATC TGTCTTGAGT
    AAACATTATG AAGCCATACT AGAAGCTGCT AGTGTGAAGA TCAGTGAAGT TGAAATTGAA
    TGGAGCATGT TGAAATTAGA GCTATATAAC AGATTTCAGA ACATCCAGAC CTTGACATGG
    GATTCGGTGA ATGCACATTA TTCAAAGAAG TATCCCAGTA TCCTGGCATT GGTAGATCTG
    ATCCTAACTC TGCCAGCAAG CTCAGCTGAA ATAGACCAAG GCTCTGGAAA AGAAAGTCAT
    CATGATGCAT CTGCACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132999.1
    CDS35..6175
    Translation

    Target ORF information:

    RefSeq Version XM_015277513.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277513.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCCGAGA TTTTGTGCGA GTGGCTGAAC GAGGAGGTGA AACTTTCCCG GAGCGTGGTT 
    CCAGGATCAC TTTCAGAAGA ATTTTCTACT GGCTACCTCT TTGGAGAACT TCTGCACAAG
    TATGGTCTTC AGGATGACTT TAATCAATTT TCACAGAGCA GGGCTGCAAA TGCAAAGCTT
    AACAATTTTT CTCTCCTGGA GCCTACGCTT CAACTGCTTG GAGTACAGTT TAATGAGAAC
    GTGGCCCACG ACATCATGAT AGGAAAGCGG GGGGCTGCAA CAAAACTTTT ATATGAATTG
    TACATTGCTT TAGAAAAAAA AAGAAAGACA AAGCTCACTG GAGTAGCTAT GGAAGCAATG
    AGACCTGCAG CACCTGCAAG GTTCAAGTCT ATTGGAAGTG TTTCATTTCG AGAGCGTTTG
    AAATCTTTAA CATCACATCA GGCTGATTTG CAGCTATGGC AGGTTTCAGA TCATTTTGAA
    ATGAAATCTA AAGCAATAGA AGATAAAATA GCTCGTATAC ATATTTCAGA ACAACAGAAA
    TCTCTAAAGC CTCAAGAGGA ACAAAGAGCC CAAGACTCTG AAAAGCATCG CATAGGAAGA
    AGACAAAATG AAATAATGGC CCGGATTCAA ACAGCCGTTA TACAAGTTCC AAAGCCATCA
    CTAAGCCACA CCTTAAAAAG TAACGAAGCA AAAAAAATGC TAAAGAAGAA AAGAGAAGCG
    GAGGACACTT ACAAGGAGAT TAACAAGTTT GAGAAGTCCC TGGTAGGAAA TGCTTTTGAA
    GTACACAGCC AAGTCACTGA TAGTGAAATA CAAGAATCAT TGCAAACACA GAAGTTGCAA
    GAAACAACCC TAGAAACTAC AGCACAGGCA GCAACTGAGC TATTACCTAC TTATTCTGAT
    GATGACTATG TAAGAAAAAT TCAAAAATAT TTGGAGGAAG ATATGTTTGC TAGAGAACAA
    CGAGAAAAGA GACGTCGTAA AATGTTAATG GAACAGTTGA TAGCACATGA AGCAGAAGAG
    GAGATTTACC AGGAGGAGCA ACTTGTTTAC AGGCTAATGA GACAATCTCA GCAGGAGCGG
    AGGATTGCTG TTCAACTCAT GCATGTTCGA CATGAGAAAG AGGTGTTAAA ACAAAATAGA
    ATTTTCAGGG AAAAGCAATA TGAAGAAAGA CGACTGAAAG AGTTCCAGGA AGCTCTTGAC
    AGAGAGGCGG CTCTTGCAAA ACAAGAAAAA ATTGATTATG AAGAACAAAG TGTCAAGGAA
    AGAGAGCGTC ATGAGAAAAT TGCCATTGAA AGAGCTCAAG CTCGTTATAA AAAGCATTAT
    TCTGCATGTT GGGAAGTGAT TGATCAGATT GTTGATTTGT CAACAAAAAT AGGAGAGTAT
    CGAGTATTGA CAAACAACAA AGTTCCGTTA AAACTGATCT GTGATTGGAA GGAATTATTT
    TTTAATGGAA ACTCAATATA TGAAAAAGCC TCAATTAAAA CTTTGCCAAG TGAACCAAGC
    CCAGAACAAC TTCTGGAGTT GAATAAAATG GATCTATTAG ATGAAAAAGA TTATGATGAA
    TACAAGAATA TGACAGGGGA ATGGTGCCCA ACTGAAGAGA ACAGAGAAAA TAAATCTCTT
    AATAATAACA TATTGGGTCA TGTTCTTCAT AGACTGATGG AGATTTTCTA TCCTTCAAAA
    CCCAGATGTA CACTACCTGT ACTTCCTTCA TTTCCCATCA AGGGATGTAT TCTGGGAAAA
    GTTTTTAGTG GAAAAACTAC CTGTGTAAAG TTTCTTGAAA AAGTTTGCAA TATTCAGGTA
    CTGTCTGTTG ATACTCTGGT TCAGGAGGCC ATCCAAGCTT TTCTTAATAA TGAAATGAAA
    ACTGAACACA GCTTGATTTC ACAGGAAGCA GAGAGTTCTG CAAAACAATA CAAGGTCCAG
    AGGAACTTCT CAGAAACATC AATGAAATAT CTAACAGACA CATTTACTAA TGGAACCAAA
    GGTAATCATC CTTTCAAAAA GCACCCATCT GATCAGGAAA CTGTATCAGA CCACAAAGAG
    AATGACATAT CCAAGCTATC TGTACGTGCT CAACTTGGTG CTGCATCTCA GAAATTGTTG
    AAGAAAGGAA AAAGTATTCC TGATGAATTA CTAGTTGACA TCCTATTGGA AGCCATTAAG
    CAAACACCAC CAGAGAAAGG ATGGATTCTG GATGGATTTC CAACGACAAT TAATCAGGCA
    AGGCTATTTG AAAAAGCCTG TACAGGGATT GATCCAGATG AAACAGAAGC AAAATATGTG
    AACTGTGGAA AGCTCTCACT TGTAACAAAT CCTAGAACAC CTAGCAAGCC TCCTGTCACC
    TTACCAGCAT TTGATGTTTC TATATTATTG GATATTTCAG ACACCACAGT ACTGAAACGT
    GTGGCTAGCT TGATGTCAGA TAAATCAAAG CCCTCTGAAA TTGAACAGGA GGACAGCAGT
    CAAAATTCAG ATGTTAAAAT CGTAGAAGAG AAGACTGACT TAGCAAGGGA CCAGGTGCTG
    CATAGACTTT CAGGCTTCCT AGACACATGG CCAAAATTAG AGAACTGGTT TTCAGACCAT
    CAGAATACTC TAGTGAAGGT CAATGCAGAG ATGGAAGAAA GTACTGTGTG CAAAACTGTA
    AAGGAAATTC TGATTGAAGA AATTTACAAG AAGCAGAATA GAAGGTATGT ACAAGAAAAA
    TCACCAGAAG AAAAGGCTTT GCCAGTTACA CCTCAAGATC TGCTTTCCCC TGTTTCTGTG
    ACACCACTGA TTAAGCCAGG AACAGATGAA TGGATTTATG TAGATGAACC ACTTCCAAAG
    GAGATACCTG ATTTTTTAGT ACCTTATTGG GAAATGGTAG AAAATGTGTA TGTGAATACC
    ATCAAAACTG TACTTAGATG TCTACGGGAC GAACAGTACT CTGTGATATA TTATTTAGCA
    GATATTAGAA AAAATTTCCA AGATTATTTA AAGCATCCAG ATCATAAGCA GGAGTTTGTA
    TCTCAGTGGC AGTCAGATTT TAATTCAATT CCTGATGACC TGCGGGAAGA TGAGGAAACA
    AAAGCAGAAC TACACCAAAG AGTAACTGAC TTAAGAGATC TTCTCTGGGA TATCTGTGAT
    AAAAGAAGGG AAGAAGCAGA GCAAGAACGT ACTGATATCA TGAATGATGG CTGGTTGCGA
    GATCATAAAG GGATTGCAAT AAACCATTTT TTTTCACTTA TGCAGGTTGA AGTGGATCGT
    TTCCAAGATA CAAAGAGACT TCTTCATGAC TATTATAGAG CAATGGAAGG AAAAATCCCA
    ACAGAGGACA GACAAAGTTT TACTAGACTT CCATTGCTAG ATATTATTGA TAAAGAGCAG
    CAAGAAGATC AGAATGAATC AAGAAGGATT CCTCTAGTTT CTTATAAACT GCCTTCACCA
    GAAACAAATA TTACTAAGTC AAAAAGCAAG GGAAATCTAC TGAAGAGTGT TAAGGATGAG
    AATTCTTCTG AAAATGTAGT GGTCGCTCTT GGGAAAGATG AAAATGTTAT TCGTGATACG
    TGGCAAACAG CAGTAACAGC AGTATCAGAT ATGGTAAGAG CAGAAATACA GTCAAAAGAG
    GTGAAGGAAG AAGGAGAAAA GCAGACAGAA GTGAAAGAAG ATACAAAGTC TTCTCAAACA
    ATATCAAGCA AATCTACTGG GAAGGATGCC AAAGACACCA AAAAATCTCC CAAATCACCT
    ACTAAAACGA AAGGACCTCG CTCCACTGCA TCTGCAGCTG AAGACAGTCC AGTTCCGTTA
    ACCGCTGAAG AACTGAAGAA ACAAAAACTG ATACTTAAAA TACAGCAAGA ATATTTTGCT
    GCCCTGAAAC ATGAAGAGAA AGCTACAAAA ACTCGACTCA AACTTATAAA AGCAAAAGCT
    TTAGCATATG TTGAAGAGTT GACAATGAAA GCAGAAGAAG CTTATAAAGA TATGGAAAAG
    TGGCTTGGAG AAAGGTTCCT AGCAGAAATG TCCAGTGTTG AAAAGCTGAT TGAGGTAGGA
    CGACACCATA TTGAAAGCTC TAGCAAAATC CAGTATGAAA TGAATTTAGA AGAAACAGAT
    TTCTTCATCA GCAGTGATGT AAAAGTGATT CCTGATCCTG TTCCTCCACC ACAAGTTCCA
    CTAATAGAGA CTTCTGAAAG TAGTACTCTA ACTATTTCGC AACTTAACAC ATTTCATAAA
    CATTTTCTAC AAGTGGCACC TAAAGCCATA AGAGCACTGA AGCAATTTAA AGGCAGGAGT
    AATTCTTCTA GCAAGAGAGA AGTTGATGAC AGAGGGAAGG AAAAGAAAGA TTTGGAACAT
    CCAAGAATAA GGAAGAATCG CCGGTTTCCA AAAATATGGC TAACAAAATA CTCCTGGTTA
    AAATATGATG ACGAAAGGGG AATAATGTTC TGTGCATTAT GTCGCAAGCA TAATGTGGAC
    CTGGGGGAAA GCATTCATAA TTTTTGTTCT GGTACTGATG ACTTCAAACT GGAATTTATA
    AATACACACC AGAACAGTGA AGCTCATGCC TGGGCTACCT GCATGGAGGC TGCCAGTACT
    GCTTCACCAA ATACAGTTTC TGCTGAGCAG ACATTGAAGA GTATGAACTC CATAACAATA
    GGAAGAGTAG AAAGTACTTT TAGAACATGC CATGCAATTG CTAAATCTGT TCAGCCCTTT
    ACAGACCTGG ACTGGATTTA TAAATCAGAT GATGTGAAAG GAACAGATAT TGGTTCTCTA
    TTCAGAAATT ATAAGTCAGC AAGAATGTTT AAACATTTTA TGGCTGAAGC AGAAAGAAGA
    GCTCTAAAAG AGGAACTGGA GAATTGTAAA TTCTTTTCTC TTATTAGTGA TGAATTCACA
    GATAGTATAT CCAAATCAGC TGCAGTTGTC TATGTGCACT TTGCAAATGA AGGAAAAGTT
    CACTGCCAGA TTGTTGGGGT TCAACCTGTT CAAAAAAATG ATGCTTCAAC TATAAAAAGT
    GCAATTGAGG AAACATTTCA AATAAATCTT CAGCTTAGCT TGGCAAGCCA AGACTGGTCA
    AGAAAATTAG TTGGATTTGG AAGCGAGGAG ACAGATGTAA TGACTGGTCA AAATAATGGG
    GTAGCTAATC TCATGAGGGA AATTCAACCT TGTGTGCAGT CTGTGCATTG TTTTGCTCAC
    CGCCTAAGGC TGGCATACAA GGGAGCACTG GAAAACATTC AGCTATACAG CTTAACCAAT
    GGCTTGAGAA GTATGTACTA CTTTTATCAT AACTCAACTC TAAATAAGAA GAATCTCAAA
    GCTATATATG AAGCCATCAA GTTACATCCA GCTATTCCAT CTTGCACTGG GGGCTCCCAG
    TGGTTTTCTC GCCTACAGAC AGCTCTACAA ATTCTCCTTA AAGGTCATCC TGCATTTGTT
    CTACATCTGG ATAAGACTAA AAGAGATTCA AGGTCCTCAA ATAGGCAGAA AGTCAAAGGA
    CTATTGTATC TCCTTCTGAA AATGGAGATA GTCAAGTTTT CCCATTTCTT GTTGGATGTC
    ATCAATGTCC TCAGCATTCT GTTACATATT ACTGTGGATC ACAATTCCTC CATTGCAGAT
    ATATTTGCAA CTATGCAGTC AACTGTGGAA ACACTCCAAA TGTATCAAGC AAGAGCTGGT
    CCAATGGAAC GCTTAATGAA GGCCATTCAA CATTTTCATG GTCACCAGCT TGTTGGAAAT
    GGAAATATTT CAGCAGTACG AACCAAAGTA CCAAGTAATG TGGTGAAGAG GTTAAGTGAT
    TGTTTCTGTG ATGCTAATCA AGGTGTCTTG AAAGCAACTC TTATTGGAAG TTTTAATCTA
    TGGCCTGACA AAATGAAACA AGTTATAGTA TTTGGTGAAA AGGAAGTATC TGTCTTGAGT
    AAACATTATG AAGCCATACT AGAAGCTGCT AGTGTGAAGA TCAGTGAAGT TGAAATTGAA
    TGGAGCATGT TGAAATTAGA GCTATATAAC AGATTTCAGA ACATCCAGAC CTTGACATGG
    GATTCGGTGA ATGCACATTA TTCAAAGAAG TATCCCAGTA TCCTGGCATT GGTAGATCTG
    ATCCTAACTC TGCCAGCAAG CTCAGCTGAA ATAGACCAAG GCTCTGGAAA AGAAAGTCAT
    CATGATGCAT CTGCACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.