FREM1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM1
Entrez Gene ID	427355
Full Name	FRAS1 related extracellular matrix 1
General protein information	Preferred Names FRAS1 related extracellular matrix 1 Names FRAS1-related extracellular matrix protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004949102.2	XP_004949159.2	FRAS1-related extracellular matrix protein 1 isoform X1
XM_015280193.1	XP_015135679.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_004949102.3	XP_004949159.2	FRAS1-related extracellular matrix protein 1 isoform X1
XM_015280193.2	XP_015135679.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_015280194.2	XP_015135680.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_004949101.2	XP_004949158.2	FRAS1-related extracellular matrix protein 1 isoform X3
XM_015280194.1	XP_015135680.1	FRAS1-related extracellular matrix protein 1 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FREM1	XP_520492.3
Canis lupus familiaris (dog)	FREM1	XP_005626693.1
Homo sapiens (human)	FREM1	NP_659403.4
	FREM1	XP_001110764.1
Rattus norvegicus (Norway rat)	Frem1	NP_001178686.1
Bos taurus (cattle)	FREM1	NP_001179924.1
Danio rerio (zebrafish)	frem1a	NP_001177237.1
Mus musculus (house mouse)	Frem1	NP_001185740.1
Gallus gallus (chicken)	FREM1	XP_424932.2
Xenopus tropicalis (tropical clawed frog)	LOC100488523	XP_002939136.2

Gene Ontology

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa48012	XM_015280194.2 Latest version!	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48010	XM_015280193.2 Latest version!	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa48011	XM_004949101.2 Latest version!	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa48009	XM_004949102.3 Latest version!	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
View Old Accession Versions >>
OGa48009	XM_004949102.2	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa48010	XM_015280193.1	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa48012	XM_015280194.1	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM1 ( XM_015280194.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135680.1 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa48012
Clone ID Related Accession (Same CDS sequence)	XM_015280194.2 , XM_015280194.1
Accession Version	XM_015280194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATC AAGAACACAT GCTGAAGACT ACCTTGCCTC TCTTTGCCAG ATTCATCATC 
AGCAATGGAC TGAGGACTAA ACATGGGACA TTCATGATCT CCTTGGAGGC AGTTGACCAA 
ACGTTGCCTA CACTATCTAG GAACAGGGGG TTAAGATTAG TAGAAGGCAA TATGGTACTC 
CTTTCTCCTG ATGTCCTGCA GCTTACAGAC TCTGACACTG CCAAAGAAAA CCTTACCTTC 
ATCTTGGCTC AGCTCCCCCA GTATGGTCAT CTCTATTACC GAGGGGCTGT ACTACTGCAG 
TACAGTTTCA CCCAACGTGA TGTGGACAAT ATGGATGTTG CGTATAAGCA CAGAGGTGGG 
GATTCTCGGG TTGACAAATT TACATTTGTT GCAACAGATA GGACTAATCA AGGCTTCATC 
GTGAATGGGA GGGTGCAGAT CGAGCCAGTG GCATTCACCA TTCAGGTAGA TCATTTGGAC 
AAAATGGCAC CCAAAATCAT TCATCTCCGT TGCTTATCTG ATGTGGAAAT GCTGAAGAAT 
GGCAATTATG GGATCTATAT TACTTCCAGG TCCCTGAAGG CGACTGATCC CAACACAGAG 
GATGACAAAA TATTTTTTAA GATCTTACAA GGTCCTCGTT ATGGCTACCT GGAAAATATA 
ACAACAGGTG GACTTATCCA TGAAGGGTTT AGCCAAAAAG ACTTAAATAG CAAAATTATA 
TTTTATGTCA TCAATCCACT GTGGGAAGTG CATTCAGACA ACTTGGAGTT TCAGGTCACA 
GACACCAATG GAAATTCAGC GATTCCCCAA ATGCTGGAGC TACGGTGGTC TCGCATTGAA 
ATGCAGCAGA CCGAATATGA AGTGTGTGAG AACGTGGGGA CGGTGTCCCT GAAAATCACC 
AGGGCAGGAC ACTCTGCTGA CTCTGCTTTT ATCGCAGTGA AGGTCAATGA AATATCTGCT 
GTGCTGGGAA AGGACTTTAC AGTGACTCCC TCCAAACTTA TTCAGTTTGA TCCAGGAATG 
TCAACCAAGA TGTGGAATAT AGCAATTACT TATGACAGAT TAGAGGAAGA TGATGAAGTC 
TTTGAAGTAG TTCTGAACTC CTCTGTGAAT GCTGTTCTTG GCACCAAGAC AAAAGCTGTA 
GTGAAAATTC TGGACTCAAA AGGAGGTCAG TGCAGTACTT TACATTCTTC CAGCCAAAGC 
AAGCAAGACT TCTGGAGGAG AGGCACACTG TTTGCAGTTT CCTCAGGCTC CTCATCACCT 
CCCAGGCCTG GCAATGTTCA CTTGGAAGGG ATCTCACCAC CTCCTTCCAA AAGGATGAGA 
GAACACAGAG GTGACACACT GCAGGACCGC AGGTTGGCGT ATCAGTCAAG GGCCAAACTG 
AGAGTGACTG GAAATGGCAG AATAGTTCAT CCTTCATCTA TATTTAGAAA TGGAACAGAT 
GTGGTTTACA AATACCATGG GATGGTATCA CTCAAAATAG AGGGTGACAC CTCATCAGCT 
AAAGCAAACA AGAAGGCTGA AGTGTCAGTA ATTAACCAAG CACAACCACA GATAAATGCC 
ATTTCCACTA GGAAGACAGA AATCCCACAA GCTGATAAGG CAGAACTGGC ATCTGAAGCA 
AGCTCAAGAC GTCAGGACTT TCACTCCTTT TCAAGGGCCT GTACACCAGA TTTGAAGGGG 
CTCATGCATT ATGAAGAAAG CACTCAGAAG TTATTTCAGT GTGACGGGAT AGAATGGAAA 
TTTTGGAGTT CCCAAAGCAA GGAGGCAAGC ATGAAAAAAT GTCCCTCTGG ATGGCGTCAT 
CGTGATGGCA GCTGCTATTT CCTGGTAGTG AATCAAAAGG TCACCTGGGA TACTGCTGCT 
CAGGCCTGCA GAGAACAATA CCTGGGGAGT CTCACAAGTG TGGCTAATGA ACAACACATG 
CAGTGGCTGT GGGACTTCGG CGGGAGGAAG CCCTTTTGGA TAGGCTTGAA TGACCAAGTC 
AATCCTGGAC ACTGGGAGTG GAACGGAGGA GAACCTGTTA CCTACATAAA CTGGAGAAGA 
GGCTCCTATC GCTTTACAAA GAGAGGCAAC AACTGTGTAG TGGTGCAGAA GAGAGGAAAA 
TGGCAAGCAA CTGACTGCAG GAAGGCCAAA TGGAACAGCT ACATATGTTC AAGAAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135680.1
CDS	143..2305
Translation	MVNQEHMLKTTLPLFARFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKVNEISAVLGKDFTVTPSKLIQFDPGMSTKMWNIAITYDRLEEDDEVFEVVLNSSVNAVLGTKTKAVVKILDSKGGQCSTLHSSSQSKQDFWRRGTLFAVSSGSSSPPRPGNVHLEGISPPPSKRMREHRGDTLQDRRLAYQSRAKLRVTGNGRIVHPSSIFRNGTDVVYKYHGMVSLKIEGDTSSAKANKKAEVSVINQAQPQINAISTRKTEIPQADKAELASEASSRRQDFHSFSRACTPDLKGLMHYEESTQKLFQCDGIEWKFWSSQSKEASMKKCPSGWRHRDGSCYFLVVNQKVTWDTAAQACREQYLGSLTSVANEQHMQWLWDFGGRKPFWIGLNDQVNPGHWEWNGGEPVTYINWRRGSYRFTKRGNNCVVVQKRGKWQATDCRKAKWNSYICSRKL

Target ORF information:

RefSeq Version	XM_015280194.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280194.2

ORF Insert Sequence:

ATGGTGAATC AAGAACACAT GCTGAAGACT ACCTTGCCTC TCTTTGCCAG ATTCATCATC 
AGCAATGGAC TGAGGACTAA ACATGGGACA TTCATGATCT CCTTGGAGGC AGTTGACCAA 
ACGTTGCCTA CACTATCTAG GAACAGGGGG TTAAGATTAG TAGAAGGCAA TATGGTACTC 
CTTTCTCCTG ATGTCCTGCA GCTTACAGAC TCTGACACTG CCAAAGAAAA CCTTACCTTC 
ATCTTGGCTC AGCTCCCCCA GTATGGTCAT CTCTATTACC GAGGGGCTGT ACTACTGCAG 
TACAGTTTCA CCCAACGTGA TGTGGACAAT ATGGATGTTG CGTATAAGCA CAGAGGTGGG 
GATTCTCGGG TTGACAAATT TACATTTGTT GCAACAGATA GGACTAATCA AGGCTTCATC 
GTGAATGGGA GGGTGCAGAT CGAGCCAGTG GCATTCACCA TTCAGGTAGA TCATTTGGAC 
AAAATGGCAC CCAAAATCAT TCATCTCCGT TGCTTATCTG ATGTGGAAAT GCTGAAGAAT 
GGCAATTATG GGATCTATAT TACTTCCAGG TCCCTGAAGG CGACTGATCC CAACACAGAG 
GATGACAAAA TATTTTTTAA GATCTTACAA GGTCCTCGTT ATGGCTACCT GGAAAATATA 
ACAACAGGTG GACTTATCCA TGAAGGGTTT AGCCAAAAAG ACTTAAATAG CAAAATTATA 
TTTTATGTCA TCAATCCACT GTGGGAAGTG CATTCAGACA ACTTGGAGTT TCAGGTCACA 
GACACCAATG GAAATTCAGC GATTCCCCAA ATGCTGGAGC TACGGTGGTC TCGCATTGAA 
ATGCAGCAGA CCGAATATGA AGTGTGTGAG AACGTGGGGA CGGTGTCCCT GAAAATCACC 
AGGGCAGGAC ACTCTGCTGA CTCTGCTTTT ATCGCAGTGA AGGTCAATGA AATATCTGCT 
GTGCTGGGAA AGGACTTTAC AGTGACTCCC TCCAAACTTA TTCAGTTTGA TCCAGGAATG 
TCAACCAAGA TGTGGAATAT AGCAATTACT TATGACAGAT TAGAGGAAGA TGATGAAGTC 
TTTGAAGTAG TTCTGAACTC CTCTGTGAAT GCTGTTCTTG GCACCAAGAC AAAAGCTGTA 
GTGAAAATTC TGGACTCAAA AGGAGGTCAG TGCAGTACTT TACATTCTTC CAGCCAAAGC 
AAGCAAGACT TCTGGAGGAG AGGCACACTG TTTGCAGTTT CCTCAGGCTC CTCATCACCT 
CCCAGGCCTG GCAATGTTCA CTTGGAAGGG ATCTCACCAC CTCCTTCCAA AAGGATGAGA 
GAACACAGAG GTGACACACT GCAGGACCGC AGGTTGGCGT ATCAGTCAAG GGCCAAACTG 
AGAGTGACTG GAAATGGCAG AATAGTTCAT CCTTCATCTA TATTTAGAAA TGGAACAGAT 
GTGGTTTACA AATACCATGG GATGGTATCA CTCAAAATAG AGGGTGACAC CTCATCAGCT 
AAAGCAAACA AGAAGGCTGA AGTGTCAGTA ATTAACCAAG CACAACCACA GATAAATGCC 
ATTTCCACTA GGAAGACAGA AATCCCACAA GCTGATAAGG CAGAACTGGC ATCTGAAGCA 
AGCTCAAGAC GTCAGGACTT TCACTCCTTT TCAAGGGCCT GTACACCAGA TTTGAAGGGG 
CTCATGCATT ATGAAGAAAG CACTCAGAAG TTATTTCAGT GTGACGGGAT AGAATGGAAA 
TTTTGGAGTT CCCAAAGCAA GGAGGCAAGC ATGAAAAAAT GTCCCTCTGG ATGGCGTCAT 
CGTGATGGCA GCTGCTATTT CCTGGTAGTG AATCAAAAGG TCACCTGGGA TACTGCTGCT 
CAGGCCTGCA GAGAACAATA CCTGGGGAGT CTCACAAGTG TGGCTAATGA ACAACACATG 
CAGTGGCTGT GGGACTTCGG CGGGAGGAAG CCCTTTTGGA TAGGCTTGAA TGACCAAGTC 
AATCCTGGAC ACTGGGAGTG GAACGGAGGA GAACCTGTTA CCTACATAAA CTGGAGAAGA 
GGCTCCTATC GCTTTACAAA GAGAGGCAAC AACTGTGTAG TGGTGCAGAA GAGAGGAAAA 
TGGCAAGCAA CTGACTGCAG GAAGGCCAAA TGGAACAGCT ACATATGTTC AAGAAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_015280194.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135680.1 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa48012
Clone ID Related Accession (Same CDS sequence)	XM_015280194.2 , XM_015280194.1
Accession Version	XM_015280194.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764317.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAATC AAGAACACAT GCTGAAGACT ACCTTGCCTC TCTTTGCCAG ATTCATCATC 
AGCAATGGAC TGAGGACTAA ACATGGGACA TTCATGATCT CCTTGGAGGC AGTTGACCAA 
ACGTTGCCTA CACTATCTAG GAACAGGGGG TTAAGATTAG TAGAAGGCAA TATGGTACTC 
CTTTCTCCTG ATGTCCTGCA GCTTACAGAC TCTGACACTG CCAAAGAAAA CCTTACCTTC 
ATCTTGGCTC AGCTCCCCCA GTATGGTCAT CTCTATTACC GAGGGGCTGT ACTACTGCAG 
TACAGTTTCA CCCAACGTGA TGTGGACAAT ATGGATGTTG CGTATAAGCA CAGAGGTGGG 
GATTCTCGGG TTGACAAATT TACATTTGTT GCAACAGATA GGACTAATCA AGGCTTCATC 
GTGAATGGGA GGGTGCAGAT CGAGCCAGTG GCATTCACCA TTCAGGTAGA TCATTTGGAC 
AAAATGGCAC CCAAAATCAT TCATCTCCGT TGCTTATCTG ATGTGGAAAT GCTGAAGAAT 
GGCAATTATG GGATCTATAT TACTTCCAGG TCCCTGAAGG CGACTGATCC CAACACAGAG 
GATGACAAAA TATTTTTTAA GATCTTACAA GGTCCTCGTT ATGGCTACCT GGAAAATATA 
ACAACAGGTG GACTTATCCA TGAAGGGTTT AGCCAAAAAG ACTTAAATAG CAAAATTATA 
TTTTATGTCA TCAATCCACT GTGGGAAGTG CATTCAGACA ACTTGGAGTT TCAGGTCACA 
GACACCAATG GAAATTCAGC GATTCCCCAA ATGCTGGAGC TACGGTGGTC TCGCATTGAA 
ATGCAGCAGA CCGAATATGA AGTGTGTGAG AACGTGGGGA CGGTGTCCCT GAAAATCACC 
AGGGCAGGAC ACTCTGCTGA CTCTGCTTTT ATCGCAGTGA AGGTCAATGA AATATCTGCT 
GTGCTGGGAA AGGACTTTAC AGTGACTCCC TCCAAACTTA TTCAGTTTGA TCCAGGAATG 
TCAACCAAGA TGTGGAATAT AGCAATTACT TATGACAGAT TAGAGGAAGA TGATGAAGTC 
TTTGAAGTAG TTCTGAACTC CTCTGTGAAT GCTGTTCTTG GCACCAAGAC AAAAGCTGTA 
GTGAAAATTC TGGACTCAAA AGGAGGTCAG TGCAGTACTT TACATTCTTC CAGCCAAAGC 
AAGCAAGACT TCTGGAGGAG AGGCACACTG TTTGCAGTTT CCTCAGGCTC CTCATCACCT 
CCCAGGCCTG GCAATGTTCA CTTGGAAGGG ATCTCACCAC CTCCTTCCAA AAGGATGAGA 
GAACACAGAG GTGACACACT GCAGGACCGC AGGTTGGCGT ATCAGTCAAG GGCCAAACTG 
AGAGTGACTG GAAATGGCAG AATAGTTCAT CCTTCATCTA TATTTAGAAA TGGAACAGAT 
GTGGTTTACA AATACCATGG GATGGTATCA CTCAAAATAG AGGGTGACAC CTCATCAGCT 
AAAGCAAACA AGAAGGCTGA AGTGTCAGTA ATTAACCAAG CACAACCACA GATAAATGCC 
ATTTCCACTA GGAAGACAGA AATCCCACAA GCTGATAAGG CAGAACTGGC ATCTGAAGCA 
AGCTCAAGAC GTCAGGACTT TCACTCCTTT TCAAGGGCCT GTACACCAGA TTTGAAGGGG 
CTCATGCATT ATGAAGAAAG CACTCAGAAG TTATTTCAGT GTGACGGGAT AGAATGGAAA 
TTTTGGAGTT CCCAAAGCAA GGAGGCAAGC ATGAAAAAAT GTCCCTCTGG ATGGCGTCAT 
CGTGATGGCA GCTGCTATTT CCTGGTAGTG AATCAAAAGG TCACCTGGGA TACTGCTGCT 
CAGGCCTGCA GAGAACAATA CCTGGGGAGT CTCACAAGTG TGGCTAATGA ACAACACATG 
CAGTGGCTGT GGGACTTCGG CGGGAGGAAG CCCTTTTGGA TAGGCTTGAA TGACCAAGTC 
AATCCTGGAC ACTGGGAGTG GAACGGAGGA GAACCTGTTA CCTACATAAA CTGGAGAAGA 
GGCTCCTATC GCTTTACAAA GAGAGGCAAC AACTGTGTAG TGGTGCAGAA GAGAGGAAAA 
TGGCAAGCAA CTGACTGCAG GAAGGCCAAA TGGAACAGCT ACATATGTTC AAGAAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135680.1
CDS	87..2249
Translation	MVNQEHMLKTTLPLFARFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKVNEISAVLGKDFTVTPSKLIQFDPGMSTKMWNIAITYDRLEEDDEVFEVVLNSSVNAVLGTKTKAVVKILDSKGGQCSTLHSSSQSKQDFWRRGTLFAVSSGSSSPPRPGNVHLEGISPPPSKRMREHRGDTLQDRRLAYQSRAKLRVTGNGRIVHPSSIFRNGTDVVYKYHGMVSLKIEGDTSSAKANKKAEVSVINQAQPQINAISTRKTEIPQADKAELASEASSRRQDFHSFSRACTPDLKGLMHYEESTQKLFQCDGIEWKFWSSQSKEASMKKCPSGWRHRDGSCYFLVVNQKVTWDTAAQACREQYLGSLTSVANEQHMQWLWDFGGRKPFWIGLNDQVNPGHWEWNGGEPVTYINWRRGSYRFTKRGNNCVVVQKRGKWQATDCRKAKWNSYICSRKL

Target ORF information:

RefSeq Version	XM_015280194.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280194.1

ORF Insert Sequence:

ATGGTGAATC AAGAACACAT GCTGAAGACT ACCTTGCCTC TCTTTGCCAG ATTCATCATC 
AGCAATGGAC TGAGGACTAA ACATGGGACA TTCATGATCT CCTTGGAGGC AGTTGACCAA 
ACGTTGCCTA CACTATCTAG GAACAGGGGG TTAAGATTAG TAGAAGGCAA TATGGTACTC 
CTTTCTCCTG ATGTCCTGCA GCTTACAGAC TCTGACACTG CCAAAGAAAA CCTTACCTTC 
ATCTTGGCTC AGCTCCCCCA GTATGGTCAT CTCTATTACC GAGGGGCTGT ACTACTGCAG 
TACAGTTTCA CCCAACGTGA TGTGGACAAT ATGGATGTTG CGTATAAGCA CAGAGGTGGG 
GATTCTCGGG TTGACAAATT TACATTTGTT GCAACAGATA GGACTAATCA AGGCTTCATC 
GTGAATGGGA GGGTGCAGAT CGAGCCAGTG GCATTCACCA TTCAGGTAGA TCATTTGGAC 
AAAATGGCAC CCAAAATCAT TCATCTCCGT TGCTTATCTG ATGTGGAAAT GCTGAAGAAT 
GGCAATTATG GGATCTATAT TACTTCCAGG TCCCTGAAGG CGACTGATCC CAACACAGAG 
GATGACAAAA TATTTTTTAA GATCTTACAA GGTCCTCGTT ATGGCTACCT GGAAAATATA 
ACAACAGGTG GACTTATCCA TGAAGGGTTT AGCCAAAAAG ACTTAAATAG CAAAATTATA 
TTTTATGTCA TCAATCCACT GTGGGAAGTG CATTCAGACA ACTTGGAGTT TCAGGTCACA 
GACACCAATG GAAATTCAGC GATTCCCCAA ATGCTGGAGC TACGGTGGTC TCGCATTGAA 
ATGCAGCAGA CCGAATATGA AGTGTGTGAG AACGTGGGGA CGGTGTCCCT GAAAATCACC 
AGGGCAGGAC ACTCTGCTGA CTCTGCTTTT ATCGCAGTGA AGGTCAATGA AATATCTGCT 
GTGCTGGGAA AGGACTTTAC AGTGACTCCC TCCAAACTTA TTCAGTTTGA TCCAGGAATG 
TCAACCAAGA TGTGGAATAT AGCAATTACT TATGACAGAT TAGAGGAAGA TGATGAAGTC 
TTTGAAGTAG TTCTGAACTC CTCTGTGAAT GCTGTTCTTG GCACCAAGAC AAAAGCTGTA 
GTGAAAATTC TGGACTCAAA AGGAGGTCAG TGCAGTACTT TACATTCTTC CAGCCAAAGC 
AAGCAAGACT TCTGGAGGAG AGGCACACTG TTTGCAGTTT CCTCAGGCTC CTCATCACCT 
CCCAGGCCTG GCAATGTTCA CTTGGAAGGG ATCTCACCAC CTCCTTCCAA AAGGATGAGA 
GAACACAGAG GTGACACACT GCAGGACCGC AGGTTGGCGT ATCAGTCAAG GGCCAAACTG 
AGAGTGACTG GAAATGGCAG AATAGTTCAT CCTTCATCTA TATTTAGAAA TGGAACAGAT 
GTGGTTTACA AATACCATGG GATGGTATCA CTCAAAATAG AGGGTGACAC CTCATCAGCT 
AAAGCAAACA AGAAGGCTGA AGTGTCAGTA ATTAACCAAG CACAACCACA GATAAATGCC 
ATTTCCACTA GGAAGACAGA AATCCCACAA GCTGATAAGG CAGAACTGGC ATCTGAAGCA 
AGCTCAAGAC GTCAGGACTT TCACTCCTTT TCAAGGGCCT GTACACCAGA TTTGAAGGGG 
CTCATGCATT ATGAAGAAAG CACTCAGAAG TTATTTCAGT GTGACGGGAT AGAATGGAAA 
TTTTGGAGTT CCCAAAGCAA GGAGGCAAGC ATGAAAAAAT GTCCCTCTGG ATGGCGTCAT 
CGTGATGGCA GCTGCTATTT CCTGGTAGTG AATCAAAAGG TCACCTGGGA TACTGCTGCT 
CAGGCCTGCA GAGAACAATA CCTGGGGAGT CTCACAAGTG TGGCTAATGA ACAACACATG 
CAGTGGCTGT GGGACTTCGG CGGGAGGAAG CCCTTTTGGA TAGGCTTGAA TGACCAAGTC 
AATCCTGGAC ACTGGGAGTG GAACGGAGGA GAACCTGTTA CCTACATAAA CTGGAGAAGA 
GGCTCCTATC GCTTTACAAA GAGAGGCAAC AACTGTGTAG TGGTGCAGAA GAGAGGAAAA 
TGGCAAGCAA CTGACTGCAG GAAGGCCAAA TGGAACAGCT ACATATGTTC AAGAAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_015280193.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135679.1 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

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CloneID	OGa48010
Clone ID Related Accession (Same CDS sequence)	XM_015280193.1 , XM_015280193.2
Accession Version	XM_015280193.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764317.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGC ACAGCCCAGG CCAGCAGTGC AGAAATGGGA GCTGTGTGTT GGGACTGAGA 
GTCATCCATA ACACAAAAAT GAGCTGTGAA GAGTTTCTGA GGAAGGGCAT TCGGTATCAG 
CACCTTGACC CTCGCTCACC GGATATCGAT TATATCCCAG TGAGGCTGGA TCTCACAGAC 
AGCAGAAGCA AAACTCTGCA TAAGACTGAG TATGCGTGGC TCCCTGTTCG GATTAAAAGT 
GCTATGCCCA ATCAAATACC CAAAGCTGTT CCAATGACAA AGTTCATCTT GGAAGTAGAC 
CAATTTATTT TAACTCCCAT TACAACCACA ACGGTTGATG CTGAAGACAA GGAAACTCCA 
AAGTCCCTGC TGGTGTTCAA CATTACAAAG CCACCTCTGC AGGGCTTCAT TACTCACCTG 
TCGGACCACA CAAAGCCTGT TGGTTCCTTC ACATGGAAAG ACCTCAGTGA AATGCTTATT 
GCTTATCAGC CTCCAAACAA CAGCCACACA GAGAGGAGGA ATTATGAGCT GGAGGTTGAG 
GTTCATGACT TCTACTTTGA GAGGAGTTTA CCAACCATTG TGCAACTTTC TATTAGAACA 
GCAGACACAA ATGCTCCTCG GGTTTCATGG AATACAGGCC TCAGCCTCCT GGAAGGGCAA 
TCCCGGCCTA TCACATGGCA ACACCTACAG GTGGTAGACA ATGATGATAT CCAAAATGTT 
CGCTTGGTCA CAGTTGATGG CTTGCAGCAT GGGCGGCTCA CAGTGAGAGG AGGAAAAGGG 
TTCATGTTTA CAGTTTCTGA CATTCAGACT GGAGTTGTTC GCTACCATCA CGATGACAGC 
GATTCCACCA AGGATTTTGT GGTATTCAGG ATTTTCGATG GCCTGCATAG TATTCGCCAT 
AAGTTTCCAA TCAATATCCT CCCTAAAGAT GACAGTCCTC CATTTCTTAT CAGCAATGTG 
GTAATTGAAG TACAGGAGGG ACAGACGATC CTGATCCAGG GCTCCATGCT TCAAGCTTCT 
GACATGGATT CCAGTGATGA CTATATAGTA TTCAATATTA CCAAGCCACC ACAGGCTGGG 
GAAATCATGA AGAAACCAGG GCCAAACTTA ATAGGGTATC CTGTCAGCAG TTTTCTTCAG 
AGGGATCTGT TTAATGGAAT AATGTATTAT CATCATTTGG GAGGAGAAGT ATTTGAAGAT 
TCTATTGAGT TTGTGCTGTG TGATAGCCAT GACCCTCCCA ATCTCTCGGA GCCACAGGTG 
ATGACGGTGC ACATTTCACC TGTGGATGAT CAGCTGCCCA AAGAAGCCCC AGGAGTGACC 
CGCCACCTGG TTGTTAAGGA GACTGAGATT GCTCACCTAA CTAAGAAACA TCTCCACTTC 
ATAGATGTGG AAGAGCAAGA TAGGGAACTC ACGTACACCA TAACCACTTC CCCATTTTTC 
TCTTGTGTGC ACGGTCACTC AGACGCTGGG AAGCTGTTTA TGGTGGACAC CATCCCAAAA 
CTGGTCAAGG ATCCTGCTGC TCTTGCTCTG AGGTCGTTTA CTCAGCATGC TGTGAACTAT 
TTGAAAGTTG CTTACATGCC CCCACTGCAG GACATTGGGC CCAATCCTCA ACACGTCCAG 
TTTGTATTTT CTGTCAGCAA TCAGCATGGT GGCACTTTGT ATGGAATCTG CTTCAATATC 
ACAATTCTTC CTGTGGACAA CCAAGCCCCA GAGGTTCTTA CAAGCCACTT GAGGGTGGAA 
GAGGGCGGCC TGAGCCCTGT CACGGAGGGA CACATTCTTG TCTCTGACGT GGATACAAAA 
CAGGAACATC TCCTCCTCCT TCTGCAGAGA CAGCCTCAGC ATGGGGCAGT GGAGCTGGAT 
GGTGTTCCCA TGGAGGAGGG CGACAAGCTT TCCTGCGCTG ATCTGCGTAC CCTGGCAGTC 
AGTTATCGGC ATGATGGTTC TGAGACTCTT ACAGATGAAG TCCTTTTTGT AGCCACAGAT 
GGCATCCACT CCTTAGAGTT CACCCTGCAG GTCAAGGTGT TGCCCATGAA TGATGAGCCA 
CCTGTTATGC AGACAGGGCT TATCAATGTG TTAAGCTGCC CGGAGGGTGA AGAAGTGGTC 
CTCTCCTCGG AGTACATATA TGCCACAGAT GCAGACAGTG ATGACATGAA GCTGGTGTTT 
GTGCTGGTTC GCCAGCCCTC CCAGGGGGTG CTGAGGAGAG CCGGCATTGC TGTGGAGCGT 
TTCTCCCAGG CTGATGTCAT CTCTGGCTTA GTCACCTATA AGCACACTGG TGGAGAGATT 
GGCTTGACTC TTGCCTTTGA GACCTTAACT TTTGTTGTTT CTGATGATGA AGCCGGCCCA 
GATGTGAAAG ACTGCTGTTA TGATAGACCT CTTCCTCCTC CAGTTCCTCT TCACCGTCCA 
CTCCCAGTGT ACGACCTTAA CATCACTGTA CTTCCAGTGG ACAACCAACC TCCATCCATT 
GCAATTGGTG CAAGGTTTGT TGTGGAGGAG GGCTCCTCAG CAGCCCTTAC CTCTAACCAT 
TTGTTTGCCA CCGACCCTGA CACCCCTGCG GATGACTTAG AGTTTGTCTT GGTTTCTCCT 
CCTCAGTTTG GTTATATTGA AAATATACTG CCTTCTCCTG GGTTTGAGAA GAGCAACGTT 
GGTATAAGTA TAGCTTCGTT TCAGTTGAAG CACCTGAAAG CCCTGAATAT AAACTATGTA 
CAAGCCAGGC ACATGCGAAT GGAGCCAAGA GCAGACCACT TTGTACTTTA TGTCACCGAT 
GGGCTGCATC GTTCAGCAGA GATTCCATTT TTTGTGCTGA TCAACCCAAC CAATGATGAA 
GTCCCAGAAT TCATAGCAAG AAACATTACT GTTCAAGAAG GTATGATGAA AGAGCTGGAT 
CTCTCTGTTA TCAGTGCTGT TGATCTGGAT GTGCCTCAAG ACCAGTTGGT ATTTGGTGTT 
GTGCAGCAGC CTTCACATGG ATTCCTCATT AACGGAGTTC ATGGCAATGA TATTCTACAC 
TACAAAGAGT TAATTCAACA CCACAACCAT GGGTTGCTTG TTCATGACTT TTCTATGGAG 
CTACTGCAGA ATGGTATGAA ACTGATGTAC ATGCATGACG ATTCAGAAAG CCTCAGTGAC 
AGCTTTAAAA TCCAGCTATC AGATGGGAAA CATAAAGTCC TCAGAACCAT TTCAGTAAAG 
GTCATCCCAG TTAATGATGA GAAACCAGTG CTGAGCAAGA AGGATAATAT AGAGGTGCAT 
ATGGGTGAAA CTCGAATTAT TTCTAGTGCT GTTCTGTCAG CTGAAGATAA AGATACCCCT 
AGGGAGAGAA TTTACTACTT ATTTGAAAGA CTTCCAGAAA GTGGCCAGCT TCAACTCAAG 
GTAGGCAGAG ACTGGGTGAC TCTCCAAACC GGAATGAAGT GTACTCAGGA AGAACTGGAT 
ATGAACCTTG TGAGATACGT CCACACAGGA GCTATAGGGT CCAAGAACCA AGACTTGTTC 
ACATTTTATC TGTGGGATGG GTACAACAGG TCCCCAGCTG TAGACTTCTA CATAAATATC 
AAGGACATGG AAAAGGGAGA CATTGCTGTT TTCATAAAAC CCCTCAAGGT ATCAAAAGGG 
GATCGAGGCT ACATAACAAC TGATGTCCTC CTTTCACTGG ATGGTACTGA CAAGCCTGAA 
GAGCTTCTCT ATGTGATAAC TTCCCCACCT CAGTATGGAC AAATAGAGTA TGCTAGCTAT 
CCTGACATCC CCATAACGAG CTTCAGTCAA ATGGATGTAG CAAGTCAGAT AGTCTGCTAT 
GTTCACAACA CCAAGGCACA TTCAAGGCAA GATGCATTCA GATTCATCAT CAGCAATGGA 
CTGAGGACTA AACATGGGAC ATTCATGATC TCCTTGGAGG CAGTTGACCA AACGTTGCCT 
ACACTATCTA GGAACAGGGG GTTAAGATTA GTAGAAGGCA ATATGGTACT CCTTTCTCCT 
GATGTCCTGC AGCTTACAGA CTCTGACACT GCCAAAGAAA ACCTTACCTT CATCTTGGCT 
CAGCTCCCCC AGTATGGTCA TCTCTATTAC CGAGGGGCTG TACTACTGCA GTACAGTTTC 
ACCCAACGTG ATGTGGACAA TATGGATGTT GCGTATAAGC ACAGAGGTGG GGATTCTCGG 
GTTGACAAAT TTACATTTGT TGCAACAGAT AGGACTAATC AAGGCTTCAT CGTGAATGGG 
AGGGTGCAGA TCGAGCCAGT GGCATTCACC ATTCAGGTAG ATCATTTGGA CAAAATGGCA 
CCCAAAATCA TTCATCTCCG TTGCTTATCT GATGTGGAAA TGCTGAAGAA TGGCAATTAT 
GGGATCTATA TTACTTCCAG GTCCCTGAAG GCGACTGATC CCAACACAGA GGATGACAAA 
ATATTTTTTA AGATCTTACA AGGTCCTCGT TATGGCTACC TGGAAAATAT AACAACAGGT 
GGACTTATCC ATGAAGGGTT TAGCCAAAAA GACTTAAATA GCAAAATTAT ATTTTATGTC 
ATCAATCCAC TGTGGGAAGT GCATTCAGAC AACTTGGAGT TTCAGGTCAC AGACACCAAT 
GGAAATTCAG CGATTCCCCA AATGCTGGAG CTACGGTGGT CTCGCATTGA AATGCAGCAG 
ACCGAATATG AAGTGTGTGA GAACGTGGGG ACGGTGTCCC TGAAAATCAC CAGGGCAGGA 
CACTCTGCTG ACTCTGCTTT TATCGCAGTG AAGGTCAATG AAATATCTGC TGTGCTGGGA 
AAGGACTTTA CAGTGACTCC CTCCAAACTT ATTCAGTTTG ATCCAGGAAT GTCAACCAAG 
ATGTGGAATA TAGCAATTAC TTATGACAGA TTAGAGGAAG ATGATGAAGT CTTTGAAGTA 
GTTCTGAACT CCTCTGTGAA TGCTGTTCTT GGCACCAAGA CAAAAGCTGT AGTGAAAATT 
CTGGACTCAA AAGGAGGTCA GTGCAGTACT TTACATTCTT CCAGCCAAAG CAAGCAAGAC 
TTCTGGAGGA GAGGCACACT GTTTGCAGTT TCCTCAGGCT CCTCATCACC TCCCAGGCCT 
GGCAATGTTC ACTTGGAAGG GATCTCACCA CCTCCTTCCA AAAGGATGAG AGAACACAGA 
GGTGACACAC TGCAGGACCG CAGGTTGGCG TATCAGTCAA GGGCCAAACT GAGAGTGACT 
GGAAATGGCA GAATAGTTCA TCCTTCATCT ATATTTAGAA ATGGAACAGA TGTGGTTTAC 
AAATACCATG GGATGGTATC ACTCAAAATA GAGGGTGACA CCTCATCAGC TAAAGCAAAC 
AAGAAGGCTG AAGTGTCAGT AATTAACCAA GCACAACCAC AGATAAATGC CATTTCCACT 
AGGAAGACAG AAATCCCACA AGCTGATAAG GCAGAACTGG CATCTGAAGC AAGCTCAAGA 
CGTCAGGACT TTCACTCCTT TTCAAGGGCC TGTACACCAG ATTTGAAGGG GCTCATGCAT 
TATGAAGAAA GCACTCAGAA GTTATTTCAG TGTGACGGGA TAGAATGGAA ATTTTGGAGT 
TCCCAAAGCA AGGAGGCAAG CATGAAAAAA TGTCCCTCTG GATGGCGTCA TCGTGATGGC 
AGCTGCTATT TCCTGGTAGT GAATCAAAAG GTCACCTGGG ATACTGCTGC TCAGGCCTGC 
AGAGAACAAT ACCTGGGGAG TCTCACAAGT GTGGCTAATG AACAACACAT GCAGTGGCTG 
TGGGACTTCG GCGGGAGGAA GCCCTTTTGG ATAGGCTTGA ATGACCAAGT CAATCCTGGA 
CACTGGGAGT GGAACGGAGG AGAACCTGTT ACCTACATAA ACTGGAGAAG AGGCTCCTAT 
CGCTTTACAA AGAGAGGCAA CAACTGTGTA GTGGTGCAGA AGAGAGGAAA ATGGCAAGCA 
ACTGACTGCA GGAAGGCCAA ATGGAACAGC TACATATGTT CAAGAAAGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135679.1
CDS	83..6016
Translation	MLEHSPGQQCRNGSCVLGLRVIHNTKMSCEEFLRKGIRYQHLDPRSPDIDYIPVRLDLTDSRSKTLHKTEYAWLPVRIKSAMPNQIPKAVPMTKFILEVDQFILTPITTTTVDAEDKETPKSLLVFNITKPPLQGFITHLSDHTKPVGSFTWKDLSEMLIAYQPPNNSHTERRNYELEVEVHDFYFERSLPTIVQLSIRTADTNAPRVSWNTGLSLLEGQSRPITWQHLQVVDNDDIQNVRLVTVDGLQHGRLTVRGGKGFMFTVSDIQTGVVRYHHDDSDSTKDFVVFRIFDGLHSIRHKFPINILPKDDSPPFLISNVVIEVQEGQTILIQGSMLQASDMDSSDDYIVFNITKPPQAGEIMKKPGPNLIGYPVSSFLQRDLFNGIMYYHHLGGEVFEDSIEFVLCDSHDPPNLSEPQVMTVHISPVDDQLPKEAPGVTRHLVVKETEIAHLTKKHLHFIDVEEQDRELTYTITTSPFFSCVHGHSDAGKLFMVDTIPKLVKDPAALALRSFTQHAVNYLKVAYMPPLQDIGPNPQHVQFVFSVSNQHGGTLYGICFNITILPVDNQAPEVLTSHLRVEEGGLSPVTEGHILVSDVDTKQEHLLLLLQRQPQHGAVELDGVPMEEGDKLSCADLRTLAVSYRHDGSETLTDEVLFVATDGIHSLEFTLQVKVLPMNDEPPVMQTGLINVLSCPEGEEVVLSSEYIYATDADSDDMKLVFVLVRQPSQGVLRRAGIAVERFSQADVISGLVTYKHTGGEIGLTLAFETLTFVVSDDEAGPDVKDCCYDRPLPPPVPLHRPLPVYDLNITVLPVDNQPPSIAIGARFVVEEGSSAALTSNHLFATDPDTPADDLEFVLVSPPQFGYIENILPSPGFEKSNVGISIASFQLKHLKALNINYVQARHMRMEPRADHFVLYVTDGLHRSAEIPFFVLINPTNDEVPEFIARNITVQEGMMKELDLSVISAVDLDVPQDQLVFGVVQQPSHGFLINGVHGNDILHYKELIQHHNHGLLVHDFSMELLQNGMKLMYMHDDSESLSDSFKIQLSDGKHKVLRTISVKVIPVNDEKPVLSKKDNIEVHMGETRIISSAVLSAEDKDTPRERIYYLFERLPESGQLQLKVGRDWVTLQTGMKCTQEELDMNLVRYVHTGAIGSKNQDLFTFYLWDGYNRSPAVDFYINIKDMEKGDIAVFIKPLKVSKGDRGYITTDVLLSLDGTDKPEELLYVITSPPQYGQIEYASYPDIPITSFSQMDVASQIVCYVHNTKAHSRQDAFRFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKVNEISAVLGKDFTVTPSKLIQFDPGMSTKMWNIAITYDRLEEDDEVFEVVLNSSVNAVLGTKTKAVVKILDSKGGQCSTLHSSSQSKQDFWRRGTLFAVSSGSSSPPRPGNVHLEGISPPPSKRMREHRGDTLQDRRLAYQSRAKLRVTGNGRIVHPSSIFRNGTDVVYKYHGMVSLKIEGDTSSAKANKKAEVSVINQAQPQINAISTRKTEIPQADKAELASEASSRRQDFHSFSRACTPDLKGLMHYEESTQKLFQCDGIEWKFWSSQSKEASMKKCPSGWRHRDGSCYFLVVNQKVTWDTAAQACREQYLGSLTSVANEQHMQWLWDFGGRKPFWIGLNDQVNPGHWEWNGGEPVTYINWRRGSYRFTKRGNNCVVVQKRGKWQATDCRKAKWNSYICSRKL

Target ORF information:

RefSeq Version	XM_015280193.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280193.1

ORF Insert Sequence:

ATGCTGGAGC ACAGCCCAGG CCAGCAGTGC AGAAATGGGA GCTGTGTGTT GGGACTGAGA 
GTCATCCATA ACACAAAAAT GAGCTGTGAA GAGTTTCTGA GGAAGGGCAT TCGGTATCAG 
CACCTTGACC CTCGCTCACC GGATATCGAT TATATCCCAG TGAGGCTGGA TCTCACAGAC 
AGCAGAAGCA AAACTCTGCA TAAGACTGAG TATGCGTGGC TCCCTGTTCG GATTAAAAGT 
GCTATGCCCA ATCAAATACC CAAAGCTGTT CCAATGACAA AGTTCATCTT GGAAGTAGAC 
CAATTTATTT TAACTCCCAT TACAACCACA ACGGTTGATG CTGAAGACAA GGAAACTCCA 
AAGTCCCTGC TGGTGTTCAA CATTACAAAG CCACCTCTGC AGGGCTTCAT TACTCACCTG 
TCGGACCACA CAAAGCCTGT TGGTTCCTTC ACATGGAAAG ACCTCAGTGA AATGCTTATT 
GCTTATCAGC CTCCAAACAA CAGCCACACA GAGAGGAGGA ATTATGAGCT GGAGGTTGAG 
GTTCATGACT TCTACTTTGA GAGGAGTTTA CCAACCATTG TGCAACTTTC TATTAGAACA 
GCAGACACAA ATGCTCCTCG GGTTTCATGG AATACAGGCC TCAGCCTCCT GGAAGGGCAA 
TCCCGGCCTA TCACATGGCA ACACCTACAG GTGGTAGACA ATGATGATAT CCAAAATGTT 
CGCTTGGTCA CAGTTGATGG CTTGCAGCAT GGGCGGCTCA CAGTGAGAGG AGGAAAAGGG 
TTCATGTTTA CAGTTTCTGA CATTCAGACT GGAGTTGTTC GCTACCATCA CGATGACAGC 
GATTCCACCA AGGATTTTGT GGTATTCAGG ATTTTCGATG GCCTGCATAG TATTCGCCAT 
AAGTTTCCAA TCAATATCCT CCCTAAAGAT GACAGTCCTC CATTTCTTAT CAGCAATGTG 
GTAATTGAAG TACAGGAGGG ACAGACGATC CTGATCCAGG GCTCCATGCT TCAAGCTTCT 
GACATGGATT CCAGTGATGA CTATATAGTA TTCAATATTA CCAAGCCACC ACAGGCTGGG 
GAAATCATGA AGAAACCAGG GCCAAACTTA ATAGGGTATC CTGTCAGCAG TTTTCTTCAG 
AGGGATCTGT TTAATGGAAT AATGTATTAT CATCATTTGG GAGGAGAAGT ATTTGAAGAT 
TCTATTGAGT TTGTGCTGTG TGATAGCCAT GACCCTCCCA ATCTCTCGGA GCCACAGGTG 
ATGACGGTGC ACATTTCACC TGTGGATGAT CAGCTGCCCA AAGAAGCCCC AGGAGTGACC 
CGCCACCTGG TTGTTAAGGA GACTGAGATT GCTCACCTAA CTAAGAAACA TCTCCACTTC 
ATAGATGTGG AAGAGCAAGA TAGGGAACTC ACGTACACCA TAACCACTTC CCCATTTTTC 
TCTTGTGTGC ACGGTCACTC AGACGCTGGG AAGCTGTTTA TGGTGGACAC CATCCCAAAA 
CTGGTCAAGG ATCCTGCTGC TCTTGCTCTG AGGTCGTTTA CTCAGCATGC TGTGAACTAT 
TTGAAAGTTG CTTACATGCC CCCACTGCAG GACATTGGGC CCAATCCTCA ACACGTCCAG 
TTTGTATTTT CTGTCAGCAA TCAGCATGGT GGCACTTTGT ATGGAATCTG CTTCAATATC 
ACAATTCTTC CTGTGGACAA CCAAGCCCCA GAGGTTCTTA CAAGCCACTT GAGGGTGGAA 
GAGGGCGGCC TGAGCCCTGT CACGGAGGGA CACATTCTTG TCTCTGACGT GGATACAAAA 
CAGGAACATC TCCTCCTCCT TCTGCAGAGA CAGCCTCAGC ATGGGGCAGT GGAGCTGGAT 
GGTGTTCCCA TGGAGGAGGG CGACAAGCTT TCCTGCGCTG ATCTGCGTAC CCTGGCAGTC 
AGTTATCGGC ATGATGGTTC TGAGACTCTT ACAGATGAAG TCCTTTTTGT AGCCACAGAT 
GGCATCCACT CCTTAGAGTT CACCCTGCAG GTCAAGGTGT TGCCCATGAA TGATGAGCCA 
CCTGTTATGC AGACAGGGCT TATCAATGTG TTAAGCTGCC CGGAGGGTGA AGAAGTGGTC 
CTCTCCTCGG AGTACATATA TGCCACAGAT GCAGACAGTG ATGACATGAA GCTGGTGTTT 
GTGCTGGTTC GCCAGCCCTC CCAGGGGGTG CTGAGGAGAG CCGGCATTGC TGTGGAGCGT 
TTCTCCCAGG CTGATGTCAT CTCTGGCTTA GTCACCTATA AGCACACTGG TGGAGAGATT 
GGCTTGACTC TTGCCTTTGA GACCTTAACT TTTGTTGTTT CTGATGATGA AGCCGGCCCA 
GATGTGAAAG ACTGCTGTTA TGATAGACCT CTTCCTCCTC CAGTTCCTCT TCACCGTCCA 
CTCCCAGTGT ACGACCTTAA CATCACTGTA CTTCCAGTGG ACAACCAACC TCCATCCATT 
GCAATTGGTG CAAGGTTTGT TGTGGAGGAG GGCTCCTCAG CAGCCCTTAC CTCTAACCAT 
TTGTTTGCCA CCGACCCTGA CACCCCTGCG GATGACTTAG AGTTTGTCTT GGTTTCTCCT 
CCTCAGTTTG GTTATATTGA AAATATACTG CCTTCTCCTG GGTTTGAGAA GAGCAACGTT 
GGTATAAGTA TAGCTTCGTT TCAGTTGAAG CACCTGAAAG CCCTGAATAT AAACTATGTA 
CAAGCCAGGC ACATGCGAAT GGAGCCAAGA GCAGACCACT TTGTACTTTA TGTCACCGAT 
GGGCTGCATC GTTCAGCAGA GATTCCATTT TTTGTGCTGA TCAACCCAAC CAATGATGAA 
GTCCCAGAAT TCATAGCAAG AAACATTACT GTTCAAGAAG GTATGATGAA AGAGCTGGAT 
CTCTCTGTTA TCAGTGCTGT TGATCTGGAT GTGCCTCAAG ACCAGTTGGT ATTTGGTGTT 
GTGCAGCAGC CTTCACATGG ATTCCTCATT AACGGAGTTC ATGGCAATGA TATTCTACAC 
TACAAAGAGT TAATTCAACA CCACAACCAT GGGTTGCTTG TTCATGACTT TTCTATGGAG 
CTACTGCAGA ATGGTATGAA ACTGATGTAC ATGCATGACG ATTCAGAAAG CCTCAGTGAC 
AGCTTTAAAA TCCAGCTATC AGATGGGAAA CATAAAGTCC TCAGAACCAT TTCAGTAAAG 
GTCATCCCAG TTAATGATGA GAAACCAGTG CTGAGCAAGA AGGATAATAT AGAGGTGCAT 
ATGGGTGAAA CTCGAATTAT TTCTAGTGCT GTTCTGTCAG CTGAAGATAA AGATACCCCT 
AGGGAGAGAA TTTACTACTT ATTTGAAAGA CTTCCAGAAA GTGGCCAGCT TCAACTCAAG 
GTAGGCAGAG ACTGGGTGAC TCTCCAAACC GGAATGAAGT GTACTCAGGA AGAACTGGAT 
ATGAACCTTG TGAGATACGT CCACACAGGA GCTATAGGGT CCAAGAACCA AGACTTGTTC 
ACATTTTATC TGTGGGATGG GTACAACAGG TCCCCAGCTG TAGACTTCTA CATAAATATC 
AAGGACATGG AAAAGGGAGA CATTGCTGTT TTCATAAAAC CCCTCAAGGT ATCAAAAGGG 
GATCGAGGCT ACATAACAAC TGATGTCCTC CTTTCACTGG ATGGTACTGA CAAGCCTGAA 
GAGCTTCTCT ATGTGATAAC TTCCCCACCT CAGTATGGAC AAATAGAGTA TGCTAGCTAT 
CCTGACATCC CCATAACGAG CTTCAGTCAA ATGGATGTAG CAAGTCAGAT AGTCTGCTAT 
GTTCACAACA CCAAGGCACA TTCAAGGCAA GATGCATTCA GATTCATCAT CAGCAATGGA 
CTGAGGACTA AACATGGGAC ATTCATGATC TCCTTGGAGG CAGTTGACCA AACGTTGCCT 
ACACTATCTA GGAACAGGGG GTTAAGATTA GTAGAAGGCA ATATGGTACT CCTTTCTCCT 
GATGTCCTGC AGCTTACAGA CTCTGACACT GCCAAAGAAA ACCTTACCTT CATCTTGGCT 
CAGCTCCCCC AGTATGGTCA TCTCTATTAC CGAGGGGCTG TACTACTGCA GTACAGTTTC 
ACCCAACGTG ATGTGGACAA TATGGATGTT GCGTATAAGC ACAGAGGTGG GGATTCTCGG 
GTTGACAAAT TTACATTTGT TGCAACAGAT AGGACTAATC AAGGCTTCAT CGTGAATGGG 
AGGGTGCAGA TCGAGCCAGT GGCATTCACC ATTCAGGTAG ATCATTTGGA CAAAATGGCA 
CCCAAAATCA TTCATCTCCG TTGCTTATCT GATGTGGAAA TGCTGAAGAA TGGCAATTAT 
GGGATCTATA TTACTTCCAG GTCCCTGAAG GCGACTGATC CCAACACAGA GGATGACAAA 
ATATTTTTTA AGATCTTACA AGGTCCTCGT TATGGCTACC TGGAAAATAT AACAACAGGT 
GGACTTATCC ATGAAGGGTT TAGCCAAAAA GACTTAAATA GCAAAATTAT ATTTTATGTC 
ATCAATCCAC TGTGGGAAGT GCATTCAGAC AACTTGGAGT TTCAGGTCAC AGACACCAAT 
GGAAATTCAG CGATTCCCCA AATGCTGGAG CTACGGTGGT CTCGCATTGA AATGCAGCAG 
ACCGAATATG AAGTGTGTGA GAACGTGGGG ACGGTGTCCC TGAAAATCAC CAGGGCAGGA 
CACTCTGCTG ACTCTGCTTT TATCGCAGTG AAGGTCAATG AAATATCTGC TGTGCTGGGA 
AAGGACTTTA CAGTGACTCC CTCCAAACTT ATTCAGTTTG ATCCAGGAAT GTCAACCAAG 
ATGTGGAATA TAGCAATTAC TTATGACAGA TTAGAGGAAG ATGATGAAGT CTTTGAAGTA 
GTTCTGAACT CCTCTGTGAA TGCTGTTCTT GGCACCAAGA CAAAAGCTGT AGTGAAAATT 
CTGGACTCAA AAGGAGGTCA GTGCAGTACT TTACATTCTT CCAGCCAAAG CAAGCAAGAC 
TTCTGGAGGA GAGGCACACT GTTTGCAGTT TCCTCAGGCT CCTCATCACC TCCCAGGCCT 
GGCAATGTTC ACTTGGAAGG GATCTCACCA CCTCCTTCCA AAAGGATGAG AGAACACAGA 
GGTGACACAC TGCAGGACCG CAGGTTGGCG TATCAGTCAA GGGCCAAACT GAGAGTGACT 
GGAAATGGCA GAATAGTTCA TCCTTCATCT ATATTTAGAA ATGGAACAGA TGTGGTTTAC 
AAATACCATG GGATGGTATC ACTCAAAATA GAGGGTGACA CCTCATCAGC TAAAGCAAAC 
AAGAAGGCTG AAGTGTCAGT AATTAACCAA GCACAACCAC AGATAAATGC CATTTCCACT 
AGGAAGACAG AAATCCCACA AGCTGATAAG GCAGAACTGG CATCTGAAGC AAGCTCAAGA 
CGTCAGGACT TTCACTCCTT TTCAAGGGCC TGTACACCAG ATTTGAAGGG GCTCATGCAT 
TATGAAGAAA GCACTCAGAA GTTATTTCAG TGTGACGGGA TAGAATGGAA ATTTTGGAGT 
TCCCAAAGCA AGGAGGCAAG CATGAAAAAA TGTCCCTCTG GATGGCGTCA TCGTGATGGC 
AGCTGCTATT TCCTGGTAGT GAATCAAAAG GTCACCTGGG ATACTGCTGC TCAGGCCTGC 
AGAGAACAAT ACCTGGGGAG TCTCACAAGT GTGGCTAATG AACAACACAT GCAGTGGCTG 
TGGGACTTCG GCGGGAGGAA GCCCTTTTGG ATAGGCTTGA ATGACCAAGT CAATCCTGGA 
CACTGGGAGT GGAACGGAGG AGAACCTGTT ACCTACATAA ACTGGAGAAG AGGCTCCTAT 
CGCTTTACAA AGAGAGGCAA CAACTGTGTA GTGGTGCAGA AGAGAGGAAA ATGGCAAGCA 
ACTGACTGCA GGAAGGCCAA ATGGAACAGC TACATATGTT CAAGAAAGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_015280193.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135679.1 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa48010
Clone ID Related Accession (Same CDS sequence)	XM_015280193.1 , XM_015280193.2
Accession Version	XM_015280193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGC ACAGCCCAGG CCAGCAGTGC AGAAATGGGA GCTGTGTGTT GGGACTGAGA 
GTCATCCATA ACACAAAAAT GAGCTGTGAA GAGTTTCTGA GGAAGGGCAT TCGGTATCAG 
CACCTTGACC CTCGCTCACC GGATATCGAT TATATCCCAG TGAGGCTGGA TCTCACAGAC 
AGCAGAAGCA AAACTCTGCA TAAGACTGAG TATGCGTGGC TCCCTGTTCG GATTAAAAGT 
GCTATGCCCA ATCAAATACC CAAAGCTGTT CCAATGACAA AGTTCATCTT GGAAGTAGAC 
CAATTTATTT TAACTCCCAT TACAACCACA ACGGTTGATG CTGAAGACAA GGAAACTCCA 
AAGTCCCTGC TGGTGTTCAA CATTACAAAG CCACCTCTGC AGGGCTTCAT TACTCACCTG 
TCGGACCACA CAAAGCCTGT TGGTTCCTTC ACATGGAAAG ACCTCAGTGA AATGCTTATT 
GCTTATCAGC CTCCAAACAA CAGCCACACA GAGAGGAGGA ATTATGAGCT GGAGGTTGAG 
GTTCATGACT TCTACTTTGA GAGGAGTTTA CCAACCATTG TGCAACTTTC TATTAGAACA 
GCAGACACAA ATGCTCCTCG GGTTTCATGG AATACAGGCC TCAGCCTCCT GGAAGGGCAA 
TCCCGGCCTA TCACATGGCA ACACCTACAG GTGGTAGACA ATGATGATAT CCAAAATGTT 
CGCTTGGTCA CAGTTGATGG CTTGCAGCAT GGGCGGCTCA CAGTGAGAGG AGGAAAAGGG 
TTCATGTTTA CAGTTTCTGA CATTCAGACT GGAGTTGTTC GCTACCATCA CGATGACAGC 
GATTCCACCA AGGATTTTGT GGTATTCAGG ATTTTCGATG GCCTGCATAG TATTCGCCAT 
AAGTTTCCAA TCAATATCCT CCCTAAAGAT GACAGTCCTC CATTTCTTAT CAGCAATGTG 
GTAATTGAAG TACAGGAGGG ACAGACGATC CTGATCCAGG GCTCCATGCT TCAAGCTTCT 
GACATGGATT CCAGTGATGA CTATATAGTA TTCAATATTA CCAAGCCACC ACAGGCTGGG 
GAAATCATGA AGAAACCAGG GCCAAACTTA ATAGGGTATC CTGTCAGCAG TTTTCTTCAG 
AGGGATCTGT TTAATGGAAT AATGTATTAT CATCATTTGG GAGGAGAAGT ATTTGAAGAT 
TCTATTGAGT TTGTGCTGTG TGATAGCCAT GACCCTCCCA ATCTCTCGGA GCCACAGGTG 
ATGACGGTGC ACATTTCACC TGTGGATGAT CAGCTGCCCA AAGAAGCCCC AGGAGTGACC 
CGCCACCTGG TTGTTAAGGA GACTGAGATT GCTCACCTAA CTAAGAAACA TCTCCACTTC 
ATAGATGTGG AAGAGCAAGA TAGGGAACTC ACGTACACCA TAACCACTTC CCCATTTTTC 
TCTTGTGTGC ACGGTCACTC AGACGCTGGG AAGCTGTTTA TGGTGGACAC CATCCCAAAA 
CTGGTCAAGG ATCCTGCTGC TCTTGCTCTG AGGTCGTTTA CTCAGCATGC TGTGAACTAT 
TTGAAAGTTG CTTACATGCC CCCACTGCAG GACATTGGGC CCAATCCTCA ACACGTCCAG 
TTTGTATTTT CTGTCAGCAA TCAGCATGGT GGCACTTTGT ATGGAATCTG CTTCAATATC 
ACAATTCTTC CTGTGGACAA CCAAGCCCCA GAGGTTCTTA CAAGCCACTT GAGGGTGGAA 
GAGGGCGGCC TGAGCCCTGT CACGGAGGGA CACATTCTTG TCTCTGACGT GGATACAAAA 
CAGGAACATC TCCTCCTCCT TCTGCAGAGA CAGCCTCAGC ATGGGGCAGT GGAGCTGGAT 
GGTGTTCCCA TGGAGGAGGG CGACAAGCTT TCCTGCGCTG ATCTGCGTAC CCTGGCAGTC 
AGTTATCGGC ATGATGGTTC TGAGACTCTT ACAGATGAAG TCCTTTTTGT AGCCACAGAT 
GGCATCCACT CCTTAGAGTT CACCCTGCAG GTCAAGGTGT TGCCCATGAA TGATGAGCCA 
CCTGTTATGC AGACAGGGCT TATCAATGTG TTAAGCTGCC CGGAGGGTGA AGAAGTGGTC 
CTCTCCTCGG AGTACATATA TGCCACAGAT GCAGACAGTG ATGACATGAA GCTGGTGTTT 
GTGCTGGTTC GCCAGCCCTC CCAGGGGGTG CTGAGGAGAG CCGGCATTGC TGTGGAGCGT 
TTCTCCCAGG CTGATGTCAT CTCTGGCTTA GTCACCTATA AGCACACTGG TGGAGAGATT 
GGCTTGACTC TTGCCTTTGA GACCTTAACT TTTGTTGTTT CTGATGATGA AGCCGGCCCA 
GATGTGAAAG ACTGCTGTTA TGATAGACCT CTTCCTCCTC CAGTTCCTCT TCACCGTCCA 
CTCCCAGTGT ACGACCTTAA CATCACTGTA CTTCCAGTGG ACAACCAACC TCCATCCATT 
GCAATTGGTG CAAGGTTTGT TGTGGAGGAG GGCTCCTCAG CAGCCCTTAC CTCTAACCAT 
TTGTTTGCCA CCGACCCTGA CACCCCTGCG GATGACTTAG AGTTTGTCTT GGTTTCTCCT 
CCTCAGTTTG GTTATATTGA AAATATACTG CCTTCTCCTG GGTTTGAGAA GAGCAACGTT 
GGTATAAGTA TAGCTTCGTT TCAGTTGAAG CACCTGAAAG CCCTGAATAT AAACTATGTA 
CAAGCCAGGC ACATGCGAAT GGAGCCAAGA GCAGACCACT TTGTACTTTA TGTCACCGAT 
GGGCTGCATC GTTCAGCAGA GATTCCATTT TTTGTGCTGA TCAACCCAAC CAATGATGAA 
GTCCCAGAAT TCATAGCAAG AAACATTACT GTTCAAGAAG GTATGATGAA AGAGCTGGAT 
CTCTCTGTTA TCAGTGCTGT TGATCTGGAT GTGCCTCAAG ACCAGTTGGT ATTTGGTGTT 
GTGCAGCAGC CTTCACATGG ATTCCTCATT AACGGAGTTC ATGGCAATGA TATTCTACAC 
TACAAAGAGT TAATTCAACA CCACAACCAT GGGTTGCTTG TTCATGACTT TTCTATGGAG 
CTACTGCAGA ATGGTATGAA ACTGATGTAC ATGCATGACG ATTCAGAAAG CCTCAGTGAC 
AGCTTTAAAA TCCAGCTATC AGATGGGAAA CATAAAGTCC TCAGAACCAT TTCAGTAAAG 
GTCATCCCAG TTAATGATGA GAAACCAGTG CTGAGCAAGA AGGATAATAT AGAGGTGCAT 
ATGGGTGAAA CTCGAATTAT TTCTAGTGCT GTTCTGTCAG CTGAAGATAA AGATACCCCT 
AGGGAGAGAA TTTACTACTT ATTTGAAAGA CTTCCAGAAA GTGGCCAGCT TCAACTCAAG 
GTAGGCAGAG ACTGGGTGAC TCTCCAAACC GGAATGAAGT GTACTCAGGA AGAACTGGAT 
ATGAACCTTG TGAGATACGT CCACACAGGA GCTATAGGGT CCAAGAACCA AGACTTGTTC 
ACATTTTATC TGTGGGATGG GTACAACAGG TCCCCAGCTG TAGACTTCTA CATAAATATC 
AAGGACATGG AAAAGGGAGA CATTGCTGTT TTCATAAAAC CCCTCAAGGT ATCAAAAGGG 
GATCGAGGCT ACATAACAAC TGATGTCCTC CTTTCACTGG ATGGTACTGA CAAGCCTGAA 
GAGCTTCTCT ATGTGATAAC TTCCCCACCT CAGTATGGAC AAATAGAGTA TGCTAGCTAT 
CCTGACATCC CCATAACGAG CTTCAGTCAA ATGGATGTAG CAAGTCAGAT AGTCTGCTAT 
GTTCACAACA CCAAGGCACA TTCAAGGCAA GATGCATTCA GATTCATCAT CAGCAATGGA 
CTGAGGACTA AACATGGGAC ATTCATGATC TCCTTGGAGG CAGTTGACCA AACGTTGCCT 
ACACTATCTA GGAACAGGGG GTTAAGATTA GTAGAAGGCA ATATGGTACT CCTTTCTCCT 
GATGTCCTGC AGCTTACAGA CTCTGACACT GCCAAAGAAA ACCTTACCTT CATCTTGGCT 
CAGCTCCCCC AGTATGGTCA TCTCTATTAC CGAGGGGCTG TACTACTGCA GTACAGTTTC 
ACCCAACGTG ATGTGGACAA TATGGATGTT GCGTATAAGC ACAGAGGTGG GGATTCTCGG 
GTTGACAAAT TTACATTTGT TGCAACAGAT AGGACTAATC AAGGCTTCAT CGTGAATGGG 
AGGGTGCAGA TCGAGCCAGT GGCATTCACC ATTCAGGTAG ATCATTTGGA CAAAATGGCA 
CCCAAAATCA TTCATCTCCG TTGCTTATCT GATGTGGAAA TGCTGAAGAA TGGCAATTAT 
GGGATCTATA TTACTTCCAG GTCCCTGAAG GCGACTGATC CCAACACAGA GGATGACAAA 
ATATTTTTTA AGATCTTACA AGGTCCTCGT TATGGCTACC TGGAAAATAT AACAACAGGT 
GGACTTATCC ATGAAGGGTT TAGCCAAAAA GACTTAAATA GCAAAATTAT ATTTTATGTC 
ATCAATCCAC TGTGGGAAGT GCATTCAGAC AACTTGGAGT TTCAGGTCAC AGACACCAAT 
GGAAATTCAG CGATTCCCCA AATGCTGGAG CTACGGTGGT CTCGCATTGA AATGCAGCAG 
ACCGAATATG AAGTGTGTGA GAACGTGGGG ACGGTGTCCC TGAAAATCAC CAGGGCAGGA 
CACTCTGCTG ACTCTGCTTT TATCGCAGTG AAGGTCAATG AAATATCTGC TGTGCTGGGA 
AAGGACTTTA CAGTGACTCC CTCCAAACTT ATTCAGTTTG ATCCAGGAAT GTCAACCAAG 
ATGTGGAATA TAGCAATTAC TTATGACAGA TTAGAGGAAG ATGATGAAGT CTTTGAAGTA 
GTTCTGAACT CCTCTGTGAA TGCTGTTCTT GGCACCAAGA CAAAAGCTGT AGTGAAAATT 
CTGGACTCAA AAGGAGGTCA GTGCAGTACT TTACATTCTT CCAGCCAAAG CAAGCAAGAC 
TTCTGGAGGA GAGGCACACT GTTTGCAGTT TCCTCAGGCT CCTCATCACC TCCCAGGCCT 
GGCAATGTTC ACTTGGAAGG GATCTCACCA CCTCCTTCCA AAAGGATGAG AGAACACAGA 
GGTGACACAC TGCAGGACCG CAGGTTGGCG TATCAGTCAA GGGCCAAACT GAGAGTGACT 
GGAAATGGCA GAATAGTTCA TCCTTCATCT ATATTTAGAA ATGGAACAGA TGTGGTTTAC 
AAATACCATG GGATGGTATC ACTCAAAATA GAGGGTGACA CCTCATCAGC TAAAGCAAAC 
AAGAAGGCTG AAGTGTCAGT AATTAACCAA GCACAACCAC AGATAAATGC CATTTCCACT 
AGGAAGACAG AAATCCCACA AGCTGATAAG GCAGAACTGG CATCTGAAGC AAGCTCAAGA 
CGTCAGGACT TTCACTCCTT TTCAAGGGCC TGTACACCAG ATTTGAAGGG GCTCATGCAT 
TATGAAGAAA GCACTCAGAA GTTATTTCAG TGTGACGGGA TAGAATGGAA ATTTTGGAGT 
TCCCAAAGCA AGGAGGCAAG CATGAAAAAA TGTCCCTCTG GATGGCGTCA TCGTGATGGC 
AGCTGCTATT TCCTGGTAGT GAATCAAAAG GTCACCTGGG ATACTGCTGC TCAGGCCTGC 
AGAGAACAAT ACCTGGGGAG TCTCACAAGT GTGGCTAATG AACAACACAT GCAGTGGCTG 
TGGGACTTCG GCGGGAGGAA GCCCTTTTGG ATAGGCTTGA ATGACCAAGT CAATCCTGGA 
CACTGGGAGT GGAACGGAGG AGAACCTGTT ACCTACATAA ACTGGAGAAG AGGCTCCTAT 
CGCTTTACAA AGAGAGGCAA CAACTGTGTA GTGGTGCAGA AGAGAGGAAA ATGGCAAGCA 
ACTGACTGCA GGAAGGCCAA ATGGAACAGC TACATATGTT CAAGAAAGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135679.1
CDS	105..6038
Translation	MLEHSPGQQCRNGSCVLGLRVIHNTKMSCEEFLRKGIRYQHLDPRSPDIDYIPVRLDLTDSRSKTLHKTEYAWLPVRIKSAMPNQIPKAVPMTKFILEVDQFILTPITTTTVDAEDKETPKSLLVFNITKPPLQGFITHLSDHTKPVGSFTWKDLSEMLIAYQPPNNSHTERRNYELEVEVHDFYFERSLPTIVQLSIRTADTNAPRVSWNTGLSLLEGQSRPITWQHLQVVDNDDIQNVRLVTVDGLQHGRLTVRGGKGFMFTVSDIQTGVVRYHHDDSDSTKDFVVFRIFDGLHSIRHKFPINILPKDDSPPFLISNVVIEVQEGQTILIQGSMLQASDMDSSDDYIVFNITKPPQAGEIMKKPGPNLIGYPVSSFLQRDLFNGIMYYHHLGGEVFEDSIEFVLCDSHDPPNLSEPQVMTVHISPVDDQLPKEAPGVTRHLVVKETEIAHLTKKHLHFIDVEEQDRELTYTITTSPFFSCVHGHSDAGKLFMVDTIPKLVKDPAALALRSFTQHAVNYLKVAYMPPLQDIGPNPQHVQFVFSVSNQHGGTLYGICFNITILPVDNQAPEVLTSHLRVEEGGLSPVTEGHILVSDVDTKQEHLLLLLQRQPQHGAVELDGVPMEEGDKLSCADLRTLAVSYRHDGSETLTDEVLFVATDGIHSLEFTLQVKVLPMNDEPPVMQTGLINVLSCPEGEEVVLSSEYIYATDADSDDMKLVFVLVRQPSQGVLRRAGIAVERFSQADVISGLVTYKHTGGEIGLTLAFETLTFVVSDDEAGPDVKDCCYDRPLPPPVPLHRPLPVYDLNITVLPVDNQPPSIAIGARFVVEEGSSAALTSNHLFATDPDTPADDLEFVLVSPPQFGYIENILPSPGFEKSNVGISIASFQLKHLKALNINYVQARHMRMEPRADHFVLYVTDGLHRSAEIPFFVLINPTNDEVPEFIARNITVQEGMMKELDLSVISAVDLDVPQDQLVFGVVQQPSHGFLINGVHGNDILHYKELIQHHNHGLLVHDFSMELLQNGMKLMYMHDDSESLSDSFKIQLSDGKHKVLRTISVKVIPVNDEKPVLSKKDNIEVHMGETRIISSAVLSAEDKDTPRERIYYLFERLPESGQLQLKVGRDWVTLQTGMKCTQEELDMNLVRYVHTGAIGSKNQDLFTFYLWDGYNRSPAVDFYINIKDMEKGDIAVFIKPLKVSKGDRGYITTDVLLSLDGTDKPEELLYVITSPPQYGQIEYASYPDIPITSFSQMDVASQIVCYVHNTKAHSRQDAFRFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKVNEISAVLGKDFTVTPSKLIQFDPGMSTKMWNIAITYDRLEEDDEVFEVVLNSSVNAVLGTKTKAVVKILDSKGGQCSTLHSSSQSKQDFWRRGTLFAVSSGSSSPPRPGNVHLEGISPPPSKRMREHRGDTLQDRRLAYQSRAKLRVTGNGRIVHPSSIFRNGTDVVYKYHGMVSLKIEGDTSSAKANKKAEVSVINQAQPQINAISTRKTEIPQADKAELASEASSRRQDFHSFSRACTPDLKGLMHYEESTQKLFQCDGIEWKFWSSQSKEASMKKCPSGWRHRDGSCYFLVVNQKVTWDTAAQACREQYLGSLTSVANEQHMQWLWDFGGRKPFWIGLNDQVNPGHWEWNGGEPVTYINWRRGSYRFTKRGNNCVVVQKRGKWQATDCRKAKWNSYICSRKL

Target ORF information:

RefSeq Version	XM_015280193.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280193.2

ORF Insert Sequence:

ATGCTGGAGC ACAGCCCAGG CCAGCAGTGC AGAAATGGGA GCTGTGTGTT GGGACTGAGA 
GTCATCCATA ACACAAAAAT GAGCTGTGAA GAGTTTCTGA GGAAGGGCAT TCGGTATCAG 
CACCTTGACC CTCGCTCACC GGATATCGAT TATATCCCAG TGAGGCTGGA TCTCACAGAC 
AGCAGAAGCA AAACTCTGCA TAAGACTGAG TATGCGTGGC TCCCTGTTCG GATTAAAAGT 
GCTATGCCCA ATCAAATACC CAAAGCTGTT CCAATGACAA AGTTCATCTT GGAAGTAGAC 
CAATTTATTT TAACTCCCAT TACAACCACA ACGGTTGATG CTGAAGACAA GGAAACTCCA 
AAGTCCCTGC TGGTGTTCAA CATTACAAAG CCACCTCTGC AGGGCTTCAT TACTCACCTG 
TCGGACCACA CAAAGCCTGT TGGTTCCTTC ACATGGAAAG ACCTCAGTGA AATGCTTATT 
GCTTATCAGC CTCCAAACAA CAGCCACACA GAGAGGAGGA ATTATGAGCT GGAGGTTGAG 
GTTCATGACT TCTACTTTGA GAGGAGTTTA CCAACCATTG TGCAACTTTC TATTAGAACA 
GCAGACACAA ATGCTCCTCG GGTTTCATGG AATACAGGCC TCAGCCTCCT GGAAGGGCAA 
TCCCGGCCTA TCACATGGCA ACACCTACAG GTGGTAGACA ATGATGATAT CCAAAATGTT 
CGCTTGGTCA CAGTTGATGG CTTGCAGCAT GGGCGGCTCA CAGTGAGAGG AGGAAAAGGG 
TTCATGTTTA CAGTTTCTGA CATTCAGACT GGAGTTGTTC GCTACCATCA CGATGACAGC 
GATTCCACCA AGGATTTTGT GGTATTCAGG ATTTTCGATG GCCTGCATAG TATTCGCCAT 
AAGTTTCCAA TCAATATCCT CCCTAAAGAT GACAGTCCTC CATTTCTTAT CAGCAATGTG 
GTAATTGAAG TACAGGAGGG ACAGACGATC CTGATCCAGG GCTCCATGCT TCAAGCTTCT 
GACATGGATT CCAGTGATGA CTATATAGTA TTCAATATTA CCAAGCCACC ACAGGCTGGG 
GAAATCATGA AGAAACCAGG GCCAAACTTA ATAGGGTATC CTGTCAGCAG TTTTCTTCAG 
AGGGATCTGT TTAATGGAAT AATGTATTAT CATCATTTGG GAGGAGAAGT ATTTGAAGAT 
TCTATTGAGT TTGTGCTGTG TGATAGCCAT GACCCTCCCA ATCTCTCGGA GCCACAGGTG 
ATGACGGTGC ACATTTCACC TGTGGATGAT CAGCTGCCCA AAGAAGCCCC AGGAGTGACC 
CGCCACCTGG TTGTTAAGGA GACTGAGATT GCTCACCTAA CTAAGAAACA TCTCCACTTC 
ATAGATGTGG AAGAGCAAGA TAGGGAACTC ACGTACACCA TAACCACTTC CCCATTTTTC 
TCTTGTGTGC ACGGTCACTC AGACGCTGGG AAGCTGTTTA TGGTGGACAC CATCCCAAAA 
CTGGTCAAGG ATCCTGCTGC TCTTGCTCTG AGGTCGTTTA CTCAGCATGC TGTGAACTAT 
TTGAAAGTTG CTTACATGCC CCCACTGCAG GACATTGGGC CCAATCCTCA ACACGTCCAG 
TTTGTATTTT CTGTCAGCAA TCAGCATGGT GGCACTTTGT ATGGAATCTG CTTCAATATC 
ACAATTCTTC CTGTGGACAA CCAAGCCCCA GAGGTTCTTA CAAGCCACTT GAGGGTGGAA 
GAGGGCGGCC TGAGCCCTGT CACGGAGGGA CACATTCTTG TCTCTGACGT GGATACAAAA 
CAGGAACATC TCCTCCTCCT TCTGCAGAGA CAGCCTCAGC ATGGGGCAGT GGAGCTGGAT 
GGTGTTCCCA TGGAGGAGGG CGACAAGCTT TCCTGCGCTG ATCTGCGTAC CCTGGCAGTC 
AGTTATCGGC ATGATGGTTC TGAGACTCTT ACAGATGAAG TCCTTTTTGT AGCCACAGAT 
GGCATCCACT CCTTAGAGTT CACCCTGCAG GTCAAGGTGT TGCCCATGAA TGATGAGCCA 
CCTGTTATGC AGACAGGGCT TATCAATGTG TTAAGCTGCC CGGAGGGTGA AGAAGTGGTC 
CTCTCCTCGG AGTACATATA TGCCACAGAT GCAGACAGTG ATGACATGAA GCTGGTGTTT 
GTGCTGGTTC GCCAGCCCTC CCAGGGGGTG CTGAGGAGAG CCGGCATTGC TGTGGAGCGT 
TTCTCCCAGG CTGATGTCAT CTCTGGCTTA GTCACCTATA AGCACACTGG TGGAGAGATT 
GGCTTGACTC TTGCCTTTGA GACCTTAACT TTTGTTGTTT CTGATGATGA AGCCGGCCCA 
GATGTGAAAG ACTGCTGTTA TGATAGACCT CTTCCTCCTC CAGTTCCTCT TCACCGTCCA 
CTCCCAGTGT ACGACCTTAA CATCACTGTA CTTCCAGTGG ACAACCAACC TCCATCCATT 
GCAATTGGTG CAAGGTTTGT TGTGGAGGAG GGCTCCTCAG CAGCCCTTAC CTCTAACCAT 
TTGTTTGCCA CCGACCCTGA CACCCCTGCG GATGACTTAG AGTTTGTCTT GGTTTCTCCT 
CCTCAGTTTG GTTATATTGA AAATATACTG CCTTCTCCTG GGTTTGAGAA GAGCAACGTT 
GGTATAAGTA TAGCTTCGTT TCAGTTGAAG CACCTGAAAG CCCTGAATAT AAACTATGTA 
CAAGCCAGGC ACATGCGAAT GGAGCCAAGA GCAGACCACT TTGTACTTTA TGTCACCGAT 
GGGCTGCATC GTTCAGCAGA GATTCCATTT TTTGTGCTGA TCAACCCAAC CAATGATGAA 
GTCCCAGAAT TCATAGCAAG AAACATTACT GTTCAAGAAG GTATGATGAA AGAGCTGGAT 
CTCTCTGTTA TCAGTGCTGT TGATCTGGAT GTGCCTCAAG ACCAGTTGGT ATTTGGTGTT 
GTGCAGCAGC CTTCACATGG ATTCCTCATT AACGGAGTTC ATGGCAATGA TATTCTACAC 
TACAAAGAGT TAATTCAACA CCACAACCAT GGGTTGCTTG TTCATGACTT TTCTATGGAG 
CTACTGCAGA ATGGTATGAA ACTGATGTAC ATGCATGACG ATTCAGAAAG CCTCAGTGAC 
AGCTTTAAAA TCCAGCTATC AGATGGGAAA CATAAAGTCC TCAGAACCAT TTCAGTAAAG 
GTCATCCCAG TTAATGATGA GAAACCAGTG CTGAGCAAGA AGGATAATAT AGAGGTGCAT 
ATGGGTGAAA CTCGAATTAT TTCTAGTGCT GTTCTGTCAG CTGAAGATAA AGATACCCCT 
AGGGAGAGAA TTTACTACTT ATTTGAAAGA CTTCCAGAAA GTGGCCAGCT TCAACTCAAG 
GTAGGCAGAG ACTGGGTGAC TCTCCAAACC GGAATGAAGT GTACTCAGGA AGAACTGGAT 
ATGAACCTTG TGAGATACGT CCACACAGGA GCTATAGGGT CCAAGAACCA AGACTTGTTC 
ACATTTTATC TGTGGGATGG GTACAACAGG TCCCCAGCTG TAGACTTCTA CATAAATATC 
AAGGACATGG AAAAGGGAGA CATTGCTGTT TTCATAAAAC CCCTCAAGGT ATCAAAAGGG 
GATCGAGGCT ACATAACAAC TGATGTCCTC CTTTCACTGG ATGGTACTGA CAAGCCTGAA 
GAGCTTCTCT ATGTGATAAC TTCCCCACCT CAGTATGGAC AAATAGAGTA TGCTAGCTAT 
CCTGACATCC CCATAACGAG CTTCAGTCAA ATGGATGTAG CAAGTCAGAT AGTCTGCTAT 
GTTCACAACA CCAAGGCACA TTCAAGGCAA GATGCATTCA GATTCATCAT CAGCAATGGA 
CTGAGGACTA AACATGGGAC ATTCATGATC TCCTTGGAGG CAGTTGACCA AACGTTGCCT 
ACACTATCTA GGAACAGGGG GTTAAGATTA GTAGAAGGCA ATATGGTACT CCTTTCTCCT 
GATGTCCTGC AGCTTACAGA CTCTGACACT GCCAAAGAAA ACCTTACCTT CATCTTGGCT 
CAGCTCCCCC AGTATGGTCA TCTCTATTAC CGAGGGGCTG TACTACTGCA GTACAGTTTC 
ACCCAACGTG ATGTGGACAA TATGGATGTT GCGTATAAGC ACAGAGGTGG GGATTCTCGG 
GTTGACAAAT TTACATTTGT TGCAACAGAT AGGACTAATC AAGGCTTCAT CGTGAATGGG 
AGGGTGCAGA TCGAGCCAGT GGCATTCACC ATTCAGGTAG ATCATTTGGA CAAAATGGCA 
CCCAAAATCA TTCATCTCCG TTGCTTATCT GATGTGGAAA TGCTGAAGAA TGGCAATTAT 
GGGATCTATA TTACTTCCAG GTCCCTGAAG GCGACTGATC CCAACACAGA GGATGACAAA 
ATATTTTTTA AGATCTTACA AGGTCCTCGT TATGGCTACC TGGAAAATAT AACAACAGGT 
GGACTTATCC ATGAAGGGTT TAGCCAAAAA GACTTAAATA GCAAAATTAT ATTTTATGTC 
ATCAATCCAC TGTGGGAAGT GCATTCAGAC AACTTGGAGT TTCAGGTCAC AGACACCAAT 
GGAAATTCAG CGATTCCCCA AATGCTGGAG CTACGGTGGT CTCGCATTGA AATGCAGCAG 
ACCGAATATG AAGTGTGTGA GAACGTGGGG ACGGTGTCCC TGAAAATCAC CAGGGCAGGA 
CACTCTGCTG ACTCTGCTTT TATCGCAGTG AAGGTCAATG AAATATCTGC TGTGCTGGGA 
AAGGACTTTA CAGTGACTCC CTCCAAACTT ATTCAGTTTG ATCCAGGAAT GTCAACCAAG 
ATGTGGAATA TAGCAATTAC TTATGACAGA TTAGAGGAAG ATGATGAAGT CTTTGAAGTA 
GTTCTGAACT CCTCTGTGAA TGCTGTTCTT GGCACCAAGA CAAAAGCTGT AGTGAAAATT 
CTGGACTCAA AAGGAGGTCA GTGCAGTACT TTACATTCTT CCAGCCAAAG CAAGCAAGAC 
TTCTGGAGGA GAGGCACACT GTTTGCAGTT TCCTCAGGCT CCTCATCACC TCCCAGGCCT 
GGCAATGTTC ACTTGGAAGG GATCTCACCA CCTCCTTCCA AAAGGATGAG AGAACACAGA 
GGTGACACAC TGCAGGACCG CAGGTTGGCG TATCAGTCAA GGGCCAAACT GAGAGTGACT 
GGAAATGGCA GAATAGTTCA TCCTTCATCT ATATTTAGAA ATGGAACAGA TGTGGTTTAC 
AAATACCATG GGATGGTATC ACTCAAAATA GAGGGTGACA CCTCATCAGC TAAAGCAAAC 
AAGAAGGCTG AAGTGTCAGT AATTAACCAA GCACAACCAC AGATAAATGC CATTTCCACT 
AGGAAGACAG AAATCCCACA AGCTGATAAG GCAGAACTGG CATCTGAAGC AAGCTCAAGA 
CGTCAGGACT TTCACTCCTT TTCAAGGGCC TGTACACCAG ATTTGAAGGG GCTCATGCAT 
TATGAAGAAA GCACTCAGAA GTTATTTCAG TGTGACGGGA TAGAATGGAA ATTTTGGAGT 
TCCCAAAGCA AGGAGGCAAG CATGAAAAAA TGTCCCTCTG GATGGCGTCA TCGTGATGGC 
AGCTGCTATT TCCTGGTAGT GAATCAAAAG GTCACCTGGG ATACTGCTGC TCAGGCCTGC 
AGAGAACAAT ACCTGGGGAG TCTCACAAGT GTGGCTAATG AACAACACAT GCAGTGGCTG 
TGGGACTTCG GCGGGAGGAA GCCCTTTTGG ATAGGCTTGA ATGACCAAGT CAATCCTGGA 
CACTGGGAGT GGAACGGAGG AGAACCTGTT ACCTACATAA ACTGGAGAAG AGGCTCCTAT 
CGCTTTACAA AGAGAGGCAA CAACTGTGTA GTGGTGCAGA AGAGAGGAAA ATGGCAAGCA 
ACTGACTGCA GGAAGGCCAA ATGGAACAGC TACATATGTT CAAGAAAGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_004949101.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004949158.2 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

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CloneID	OGa48011
Clone ID Related Accession (Same CDS sequence)	XM_004949101.2
Accession Version	XM_004949101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764317.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004949101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TTGAGAAAAC ATGGCTGTTG CTATTGCTGC CTGTTCTCCT AAGAGTTGTC 
TGCTCCTCTT TTGTAAGTGT CAACCTTGGC ATTAAAGTGA TGAAGGGACA GTCTGTTTTC 
CTGTCAGAAG ATGACCTCAA GTTTTCCATC CCCAGAGAGA AGGATGTGTG CAAAGTCGAA 
GTTGTGAGAA ATGAGCCAAT AACGCAGAGG GTTGGGAAGC TAACTCCACA GGTTTTTGAC 
TGTCACTTTC GCCTTAATGA AGTCAAGTAT ACCCACAACG GTTGTCCGAT CTTAAATGCA 
GACACAGTCA TGCTTAGGCT GTACAGGTTT ACAGAAAATG AAACATTTGT AGAGACATTC 
ACCCTCCACG TACAGTTACT TGAGCCAGAC TGTAACATCA TCAAAATGCG CTCCCGAGCT 
TTGGAGGTGG CTGAGTACTA CGGCCTGTCC AGGGCAATTG ACAAGAACAG CCTCACTTTT 
GACTACAGCA GGAAGATGAA TTTGGAATGC ACCATTTCCA TAGCCTCTTC AGAAACCCAC 
CTCCCTGCTC ACGGCCAGCT CATCATGGGA GACACGCGGC AGGAAAAGCT TCGAGGGGAT 
CAACCACAGA GCTTCCTCCT TGGCCCTAAG CACAGCCCAG GCCAGCAGTG CAGAAATGGG 
AGCTGTGTGT TGGGACTGAG AGTCATCCAT AACACAAAAA TGAGCTGTGA AGAGTTTCTG 
AGGAAGGGCA TTCGGTATCA GCACCTTGAC CCTCGCTCAC CGGATATCGA TTATATCCCA 
GTGAGGCTGG ATCTCACAGA CAGCAGAAGC AAAACTCTGC ATAAGACTGA GTATGCGTGG 
CTCCCTGTTC GGATTAAAAG TGCTATGCCC AATCAAATAC CCAAAGCTGT TCCAATGACA 
AAGTTCATCT TGGAAGTAGA CCAATTTATT TTAACTCCCA TTACAACCAC AACGGTTGAT 
GCTGAAGACA AGGAAACTCC AAAGTCCCTG CTGGTGTTCA ACATTACAAA GCCACCTCTG 
CAGGGCTTCA TTACTCACCT GTCGGACCAC ACAAAGCCTG TTGGTTCCTT CACATGGAAA 
GACCTCAGTG AAATGCTTAT TGCTTATCAG CCTCCAAACA ACAGCCACAC AGAGAGGAGG 
AATTATGAGC TGGAGGTTGA GGTTCATGAC TTCTACTTTG AGAGGAGTTT ACCAACCATT 
GTGCAACTTT CTATTAGAAC AGCAGACACA AATGCTCCTC GGGTTTCATG GAATACAGGC 
CTCAGCCTCC TGGAAGGGCA ATCCCGGCCT ATCACATGGC AACACCTACA GGTGGTAGAC 
AATGATGATA TCCAAAATGT TCGCTTGGTC ACAGTTGATG GCTTGCAGCA TGGGCGGCTC 
ACAGTGAGAG GAGGAAAAGG GTTCATGTTT ACAGTTTCTG ACATTCAGAC TGGAGTTGTT 
CGCTACCATC ACGATGACAG CGATTCCACC AAGGATTTTG TGGTATTCAG GATTTTCGAT 
GGCCTGCATA GTATTCGCCA TAAGTTTCCA ATCAATATCC TCCCTAAAGA TGACAGTCCT 
CCATTTCTTA TCAGCAATGT GGTAATTGAA GTACAGGAGG GACAGACGAT CCTGATCCAG 
GGCTCCATGC TTCAAGCTTC TGACATGGAT TCCAGTGATG ACTATATAGT ATTCAATATT 
ACCAAGCCAC CACAGGCTGG GGAAATCATG AAGAAACCAG GGCCAAACTT AATAGGGTAT 
CCTGTCAGCA GTTTTCTTCA GAGGGATCTG TTTAATGGAA TAATGTATTA TCATCATTTG 
GGAGGAGAAG TATTTGAAGA TTCTATTGAG TTTGTGCTGT GTGATAGCCA TGACCCTCCC 
AATCTCTCGG AGCCACAGGT GATGACGGTG CACATTTCAC CTGTGGATGA TCAGCTGCCC 
AAAGAAGCCC CAGGAGTGAC CCGCCACCTG GTTGTTAAGG AGACTGAGAT TGCTCACCTA 
ACTAAGAAAC ATCTCCACTT CATAGATGTG GAAGAGCAAG ATAGGGAACT CACGTACACC 
ATAACCACTT CCCCATTTTT CTCTTGTGTG CACGGTCACT CAGACGCTGG GAAGCTGTTT 
ATGGTGGACA CCATCCCAAA ACTGGTCAAG GATCCTGCTG CTCTTGCTCT GAGGTCGTTT 
ACTCAGCATG CTGTGAACTA TTTGAAAGTT GCTTACATGC CCCCACTGCA GGACATTGGG 
CCCAATCCTC AACACGTCCA GTTTGTATTT TCTGTCAGCA ATCAGCATGG TGGCACTTTG 
TATGGAATCT GCTTCAATAT CACAATTCTT CCTGTGGACA ACCAAGCCCC AGAGGTTCTT 
ACAAGCCACT TGAGGGTGGA AGAGGGCGGC CTGAGCCCTG TCACGGAGGG ACACATTCTT 
GTCTCTGACG TGGATACAAA ACAGGAACAT CTCCTCCTCC TTCTGCAGAG ACAGCCTCAG 
CATGGGGCAG TGGAGCTGGA TGGTGTTCCC ATGGAGGAGG GCGACAAGCT TTCCTGCGCT 
GATCTGCGTA CCCTGGCAGT CAGTTATCGG CATGATGGTT CTGAGACTCT TACAGATGAA 
GTCCTTTTTG TAGCCACAGA TGGCATCCAC TCCTTAGAGT TCACCCTGCA GGTCAAGGTG 
TTGCCCATGA ATGATGAGCC ACCTGTTATG CAGACAGGGC TTATCAATGT GTTAAGCTGC 
CCGGAGGGTG AAGAAGTGGT CCTCTCCTCG GAGTACATAT ATGCCACAGA TGCAGACAGT 
GATGACATGA AGCTGGTGTT TGTGCTGGTT CGCCAGCCCT CCCAGGGGGT GCTGAGGAGA 
GCCGGCATTG CTGTGGAGCG TTTCTCCCAG GCTGATGTCA TCTCTGGCTT AGTCACCTAT 
AAGCACACTG GTGGAGAGAT TGGCTTGACT CTTGCCTTTG AGACCTTAAC TTTTGTTGTT 
TCTGATGATG AAGCCGGCCC AGATGTGAAA GACTGCTGTT ATGATAGACC TCTTCCTCCT 
CCAGTTCCTC TTCACCGTCC ACTCCCAGTG TACGACCTTA ACATCACTGT ACTTCCAGTG 
GACAACCAAC CTCCATCCAT TGCAATTGGT GCAAGGTTTG TTGTGGAGGA GGGCTCCTCA 
GCAGCCCTTA CCTCTAACCA TTTGTTTGCC ACCGACCCTG ACACCCCTGC GGATGACTTA 
GAGTTTGTCT TGGTTTCTCC TCCTCAGTTT GGTTATATTG AAAATATACT GCCTTCTCCT 
GGGTTTGAGA AGAGCAACGT TGGTATAAGT ATAGCTTCGT TTCAGTTGAA GCACCTGAAA 
GCCCTGAATA TAAACTATGT ACAAGCCAGG CACATGCGAA TGGAGCCAAG AGCAGACCAC 
TTTGTACTTT ATGTCACCGA TGGGCTGCAT CGTTCAGCAG AGATTCCATT TTTTGTGCTG 
ATCAACCCAA CCAATGATGA AGTCCCAGAA TTCATAGCAA GAAACATTAC TGTTCAAGAA 
GGTATGATGA AAGAGCTGGA TCTCTCTGTT ATCAGTGCTG TTGATCTGGA TGTGCCTCAA 
GACCAGTTGG TATTTGGTGT TGTGCAGCAG CCTTCACATG GATTCCTCAT TAACGGAGTT 
CATGGCAATG ATATTCTACA CTACAAAGAG TTAATTCAAC ACCACAACCA TGGGTTGCTT 
GTTCATGACT TTTCTATGGA GCTACTGCAG AATGGTATGA AACTGATGTA CATGCATGAC 
GATTCAGAAA GCCTCAGTGA CAGCTTTAAA ATCCAGCTAT CAGATGGGAA ACATAAAGTC 
CTCAGAACCA TTTCAGTAAA GGTCATCCCA GTTAATGATG AGAAACCAGT GCTGAGCAAG 
AAGGATAATA TAGAGGTGCA TATGGGTGAA ACTCGAATTA TTTCTAGTGC TGTTCTGTCA 
GCTGAAGATA AAGATACCCC TAGGGAGAGA ATTTACTACT TATTTGAAAG ACTTCCAGAA 
AGTGGCCAGC TTCAACTCAA GGTAGGCAGA GACTGGGTGA CTCTCCAAAC CGGAATGAAG 
TGTACTCAGG AAGAACTGGA TATGAACCTT GTGAGATACG TCCACACAGG AGCTATAGGG 
TCCAAGAACC AAGACTTGTT CACATTTTAT CTGTGGGATG GGTACAACAG GTCCCCAGCT 
GTAGACTTCT ACATAAATAT CAAGGACATG GAAAAGGGAG ACATTGCTGT TTTCATAAAA 
CCCCTCAAGG TATCAAAAGG GGATCGAGGC TACATAACAA CTGATGTCCT CCTTTCACTG 
GATGGTACTG ACAAGCCTGA AGAGCTTCTC TATGTGATAA CTTCCCCACC TCAGTATGGA 
CAAATAGAGT ATGCTAGCTA TCCTGACATC CCCATAACGA GCTTCAGTCA AATGGATGTA 
GCAAGTCAGA TAGTCTGCTA TGTTCACAAC ACCAAGGCAC ATTCAAGGCA AGATGCATTC 
AGATTCATCA TCAGCAATGG ACTGAGGACT AAACATGGGA CATTCATGAT CTCCTTGGAG 
GCAGTTGACC AAACGTTGCC TACACTATCT AGGAACAGGG GGTTAAGATT AGTAGAAGGC 
AATATGGTAC TCCTTTCTCC TGATGTCCTG CAGCTTACAG ACTCTGACAC TGCCAAAGAA 
AACCTTACCT TCATCTTGGC TCAGCTCCCC CAGTATGGTC ATCTCTATTA CCGAGGGGCT 
GTACTACTGC AGTACAGTTT CACCCAACGT GATGTGGACA ATATGGATGT TGCGTATAAG 
CACAGAGGTG GGGATTCTCG GGTTGACAAA TTTACATTTG TTGCAACAGA TAGGACTAAT 
CAAGGCTTCA TCGTGAATGG GAGGGTGCAG ATCGAGCCAG TGGCATTCAC CATTCAGGTA 
GATCATTTGG ACAAAATGGC ACCCAAAATC ATTCATCTCC GTTGCTTATC TGATGTGGAA 
ATGCTGAAGA ATGGCAATTA TGGGATCTAT ATTACTTCCA GGTCCCTGAA GGCGACTGAT 
CCCAACACAG AGGATGACAA AATATTTTTT AAGATCTTAC AAGGTCCTCG TTATGGCTAC 
CTGGAAAATA TAACAACAGG TGGACTTATC CATGAAGGGT TTAGCCAAAA AGACTTAAAT 
AGCAAAATTA TATTTTATGT CATCAATCCA CTGTGGGAAG TGCATTCAGA CAACTTGGAG 
TTTCAGGTCA CAGACACCAA TGGAAATTCA GCGATTCCCC AAATGCTGGA GCTACGGTGG 
TCTCGCATTG AAATGCAGCA GACCGAATAT GAAGTGTGTG AGAACGTGGG GACGGTGTCC 
CTGAAAATCA CCAGGGCAGG ACACTCTGCT GACTCTGCTT TTATCGCAGT GAAGGAATGT 
CAACCAAGAT GTGGAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004949158.2
CDS	742..6102
Translation	MKPFEKTWLLLLLPVLLRVVCSSFVSVNLGIKVMKGQSVFLSEDDLKFSIPREKDVCKVEVVRNEPITQRVGKLTPQVFDCHFRLNEVKYTHNGCPILNADTVMLRLYRFTENETFVETFTLHVQLLEPDCNIIKMRSRALEVAEYYGLSRAIDKNSLTFDYSRKMNLECTISIASSETHLPAHGQLIMGDTRQEKLRGDQPQSFLLGPKHSPGQQCRNGSCVLGLRVIHNTKMSCEEFLRKGIRYQHLDPRSPDIDYIPVRLDLTDSRSKTLHKTEYAWLPVRIKSAMPNQIPKAVPMTKFILEVDQFILTPITTTTVDAEDKETPKSLLVFNITKPPLQGFITHLSDHTKPVGSFTWKDLSEMLIAYQPPNNSHTERRNYELEVEVHDFYFERSLPTIVQLSIRTADTNAPRVSWNTGLSLLEGQSRPITWQHLQVVDNDDIQNVRLVTVDGLQHGRLTVRGGKGFMFTVSDIQTGVVRYHHDDSDSTKDFVVFRIFDGLHSIRHKFPINILPKDDSPPFLISNVVIEVQEGQTILIQGSMLQASDMDSSDDYIVFNITKPPQAGEIMKKPGPNLIGYPVSSFLQRDLFNGIMYYHHLGGEVFEDSIEFVLCDSHDPPNLSEPQVMTVHISPVDDQLPKEAPGVTRHLVVKETEIAHLTKKHLHFIDVEEQDRELTYTITTSPFFSCVHGHSDAGKLFMVDTIPKLVKDPAALALRSFTQHAVNYLKVAYMPPLQDIGPNPQHVQFVFSVSNQHGGTLYGICFNITILPVDNQAPEVLTSHLRVEEGGLSPVTEGHILVSDVDTKQEHLLLLLQRQPQHGAVELDGVPMEEGDKLSCADLRTLAVSYRHDGSETLTDEVLFVATDGIHSLEFTLQVKVLPMNDEPPVMQTGLINVLSCPEGEEVVLSSEYIYATDADSDDMKLVFVLVRQPSQGVLRRAGIAVERFSQADVISGLVTYKHTGGEIGLTLAFETLTFVVSDDEAGPDVKDCCYDRPLPPPVPLHRPLPVYDLNITVLPVDNQPPSIAIGARFVVEEGSSAALTSNHLFATDPDTPADDLEFVLVSPPQFGYIENILPSPGFEKSNVGISIASFQLKHLKALNINYVQARHMRMEPRADHFVLYVTDGLHRSAEIPFFVLINPTNDEVPEFIARNITVQEGMMKELDLSVISAVDLDVPQDQLVFGVVQQPSHGFLINGVHGNDILHYKELIQHHNHGLLVHDFSMELLQNGMKLMYMHDDSESLSDSFKIQLSDGKHKVLRTISVKVIPVNDEKPVLSKKDNIEVHMGETRIISSAVLSAEDKDTPRERIYYLFERLPESGQLQLKVGRDWVTLQTGMKCTQEELDMNLVRYVHTGAIGSKNQDLFTFYLWDGYNRSPAVDFYINIKDMEKGDIAVFIKPLKVSKGDRGYITTDVLLSLDGTDKPEELLYVITSPPQYGQIEYASYPDIPITSFSQMDVASQIVCYVHNTKAHSRQDAFRFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKECQPRCGI

Target ORF information:

RefSeq Version	XM_004949101.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004949101.2

ORF Insert Sequence:

ATGAAGCCCT TTGAGAAAAC ATGGCTGTTG CTATTGCTGC CTGTTCTCCT AAGAGTTGTC 
TGCTCCTCTT TTGTAAGTGT CAACCTTGGC ATTAAAGTGA TGAAGGGACA GTCTGTTTTC 
CTGTCAGAAG ATGACCTCAA GTTTTCCATC CCCAGAGAGA AGGATGTGTG CAAAGTCGAA 
GTTGTGAGAA ATGAGCCAAT AACGCAGAGG GTTGGGAAGC TAACTCCACA GGTTTTTGAC 
TGTCACTTTC GCCTTAATGA AGTCAAGTAT ACCCACAACG GTTGTCCGAT CTTAAATGCA 
GACACAGTCA TGCTTAGGCT GTACAGGTTT ACAGAAAATG AAACATTTGT AGAGACATTC 
ACCCTCCACG TACAGTTACT TGAGCCAGAC TGTAACATCA TCAAAATGCG CTCCCGAGCT 
TTGGAGGTGG CTGAGTACTA CGGCCTGTCC AGGGCAATTG ACAAGAACAG CCTCACTTTT 
GACTACAGCA GGAAGATGAA TTTGGAATGC ACCATTTCCA TAGCCTCTTC AGAAACCCAC 
CTCCCTGCTC ACGGCCAGCT CATCATGGGA GACACGCGGC AGGAAAAGCT TCGAGGGGAT 
CAACCACAGA GCTTCCTCCT TGGCCCTAAG CACAGCCCAG GCCAGCAGTG CAGAAATGGG 
AGCTGTGTGT TGGGACTGAG AGTCATCCAT AACACAAAAA TGAGCTGTGA AGAGTTTCTG 
AGGAAGGGCA TTCGGTATCA GCACCTTGAC CCTCGCTCAC CGGATATCGA TTATATCCCA 
GTGAGGCTGG ATCTCACAGA CAGCAGAAGC AAAACTCTGC ATAAGACTGA GTATGCGTGG 
CTCCCTGTTC GGATTAAAAG TGCTATGCCC AATCAAATAC CCAAAGCTGT TCCAATGACA 
AAGTTCATCT TGGAAGTAGA CCAATTTATT TTAACTCCCA TTACAACCAC AACGGTTGAT 
GCTGAAGACA AGGAAACTCC AAAGTCCCTG CTGGTGTTCA ACATTACAAA GCCACCTCTG 
CAGGGCTTCA TTACTCACCT GTCGGACCAC ACAAAGCCTG TTGGTTCCTT CACATGGAAA 
GACCTCAGTG AAATGCTTAT TGCTTATCAG CCTCCAAACA ACAGCCACAC AGAGAGGAGG 
AATTATGAGC TGGAGGTTGA GGTTCATGAC TTCTACTTTG AGAGGAGTTT ACCAACCATT 
GTGCAACTTT CTATTAGAAC AGCAGACACA AATGCTCCTC GGGTTTCATG GAATACAGGC 
CTCAGCCTCC TGGAAGGGCA ATCCCGGCCT ATCACATGGC AACACCTACA GGTGGTAGAC 
AATGATGATA TCCAAAATGT TCGCTTGGTC ACAGTTGATG GCTTGCAGCA TGGGCGGCTC 
ACAGTGAGAG GAGGAAAAGG GTTCATGTTT ACAGTTTCTG ACATTCAGAC TGGAGTTGTT 
CGCTACCATC ACGATGACAG CGATTCCACC AAGGATTTTG TGGTATTCAG GATTTTCGAT 
GGCCTGCATA GTATTCGCCA TAAGTTTCCA ATCAATATCC TCCCTAAAGA TGACAGTCCT 
CCATTTCTTA TCAGCAATGT GGTAATTGAA GTACAGGAGG GACAGACGAT CCTGATCCAG 
GGCTCCATGC TTCAAGCTTC TGACATGGAT TCCAGTGATG ACTATATAGT ATTCAATATT 
ACCAAGCCAC CACAGGCTGG GGAAATCATG AAGAAACCAG GGCCAAACTT AATAGGGTAT 
CCTGTCAGCA GTTTTCTTCA GAGGGATCTG TTTAATGGAA TAATGTATTA TCATCATTTG 
GGAGGAGAAG TATTTGAAGA TTCTATTGAG TTTGTGCTGT GTGATAGCCA TGACCCTCCC 
AATCTCTCGG AGCCACAGGT GATGACGGTG CACATTTCAC CTGTGGATGA TCAGCTGCCC 
AAAGAAGCCC CAGGAGTGAC CCGCCACCTG GTTGTTAAGG AGACTGAGAT TGCTCACCTA 
ACTAAGAAAC ATCTCCACTT CATAGATGTG GAAGAGCAAG ATAGGGAACT CACGTACACC 
ATAACCACTT CCCCATTTTT CTCTTGTGTG CACGGTCACT CAGACGCTGG GAAGCTGTTT 
ATGGTGGACA CCATCCCAAA ACTGGTCAAG GATCCTGCTG CTCTTGCTCT GAGGTCGTTT 
ACTCAGCATG CTGTGAACTA TTTGAAAGTT GCTTACATGC CCCCACTGCA GGACATTGGG 
CCCAATCCTC AACACGTCCA GTTTGTATTT TCTGTCAGCA ATCAGCATGG TGGCACTTTG 
TATGGAATCT GCTTCAATAT CACAATTCTT CCTGTGGACA ACCAAGCCCC AGAGGTTCTT 
ACAAGCCACT TGAGGGTGGA AGAGGGCGGC CTGAGCCCTG TCACGGAGGG ACACATTCTT 
GTCTCTGACG TGGATACAAA ACAGGAACAT CTCCTCCTCC TTCTGCAGAG ACAGCCTCAG 
CATGGGGCAG TGGAGCTGGA TGGTGTTCCC ATGGAGGAGG GCGACAAGCT TTCCTGCGCT 
GATCTGCGTA CCCTGGCAGT CAGTTATCGG CATGATGGTT CTGAGACTCT TACAGATGAA 
GTCCTTTTTG TAGCCACAGA TGGCATCCAC TCCTTAGAGT TCACCCTGCA GGTCAAGGTG 
TTGCCCATGA ATGATGAGCC ACCTGTTATG CAGACAGGGC TTATCAATGT GTTAAGCTGC 
CCGGAGGGTG AAGAAGTGGT CCTCTCCTCG GAGTACATAT ATGCCACAGA TGCAGACAGT 
GATGACATGA AGCTGGTGTT TGTGCTGGTT CGCCAGCCCT CCCAGGGGGT GCTGAGGAGA 
GCCGGCATTG CTGTGGAGCG TTTCTCCCAG GCTGATGTCA TCTCTGGCTT AGTCACCTAT 
AAGCACACTG GTGGAGAGAT TGGCTTGACT CTTGCCTTTG AGACCTTAAC TTTTGTTGTT 
TCTGATGATG AAGCCGGCCC AGATGTGAAA GACTGCTGTT ATGATAGACC TCTTCCTCCT 
CCAGTTCCTC TTCACCGTCC ACTCCCAGTG TACGACCTTA ACATCACTGT ACTTCCAGTG 
GACAACCAAC CTCCATCCAT TGCAATTGGT GCAAGGTTTG TTGTGGAGGA GGGCTCCTCA 
GCAGCCCTTA CCTCTAACCA TTTGTTTGCC ACCGACCCTG ACACCCCTGC GGATGACTTA 
GAGTTTGTCT TGGTTTCTCC TCCTCAGTTT GGTTATATTG AAAATATACT GCCTTCTCCT 
GGGTTTGAGA AGAGCAACGT TGGTATAAGT ATAGCTTCGT TTCAGTTGAA GCACCTGAAA 
GCCCTGAATA TAAACTATGT ACAAGCCAGG CACATGCGAA TGGAGCCAAG AGCAGACCAC 
TTTGTACTTT ATGTCACCGA TGGGCTGCAT CGTTCAGCAG AGATTCCATT TTTTGTGCTG 
ATCAACCCAA CCAATGATGA AGTCCCAGAA TTCATAGCAA GAAACATTAC TGTTCAAGAA 
GGTATGATGA AAGAGCTGGA TCTCTCTGTT ATCAGTGCTG TTGATCTGGA TGTGCCTCAA 
GACCAGTTGG TATTTGGTGT TGTGCAGCAG CCTTCACATG GATTCCTCAT TAACGGAGTT 
CATGGCAATG ATATTCTACA CTACAAAGAG TTAATTCAAC ACCACAACCA TGGGTTGCTT 
GTTCATGACT TTTCTATGGA GCTACTGCAG AATGGTATGA AACTGATGTA CATGCATGAC 
GATTCAGAAA GCCTCAGTGA CAGCTTTAAA ATCCAGCTAT CAGATGGGAA ACATAAAGTC 
CTCAGAACCA TTTCAGTAAA GGTCATCCCA GTTAATGATG AGAAACCAGT GCTGAGCAAG 
AAGGATAATA TAGAGGTGCA TATGGGTGAA ACTCGAATTA TTTCTAGTGC TGTTCTGTCA 
GCTGAAGATA AAGATACCCC TAGGGAGAGA ATTTACTACT TATTTGAAAG ACTTCCAGAA 
AGTGGCCAGC TTCAACTCAA GGTAGGCAGA GACTGGGTGA CTCTCCAAAC CGGAATGAAG 
TGTACTCAGG AAGAACTGGA TATGAACCTT GTGAGATACG TCCACACAGG AGCTATAGGG 
TCCAAGAACC AAGACTTGTT CACATTTTAT CTGTGGGATG GGTACAACAG GTCCCCAGCT 
GTAGACTTCT ACATAAATAT CAAGGACATG GAAAAGGGAG ACATTGCTGT TTTCATAAAA 
CCCCTCAAGG TATCAAAAGG GGATCGAGGC TACATAACAA CTGATGTCCT CCTTTCACTG 
GATGGTACTG ACAAGCCTGA AGAGCTTCTC TATGTGATAA CTTCCCCACC TCAGTATGGA 
CAAATAGAGT ATGCTAGCTA TCCTGACATC CCCATAACGA GCTTCAGTCA AATGGATGTA 
GCAAGTCAGA TAGTCTGCTA TGTTCACAAC ACCAAGGCAC ATTCAAGGCA AGATGCATTC 
AGATTCATCA TCAGCAATGG ACTGAGGACT AAACATGGGA CATTCATGAT CTCCTTGGAG 
GCAGTTGACC AAACGTTGCC TACACTATCT AGGAACAGGG GGTTAAGATT AGTAGAAGGC 
AATATGGTAC TCCTTTCTCC TGATGTCCTG CAGCTTACAG ACTCTGACAC TGCCAAAGAA 
AACCTTACCT TCATCTTGGC TCAGCTCCCC CAGTATGGTC ATCTCTATTA CCGAGGGGCT 
GTACTACTGC AGTACAGTTT CACCCAACGT GATGTGGACA ATATGGATGT TGCGTATAAG 
CACAGAGGTG GGGATTCTCG GGTTGACAAA TTTACATTTG TTGCAACAGA TAGGACTAAT 
CAAGGCTTCA TCGTGAATGG GAGGGTGCAG ATCGAGCCAG TGGCATTCAC CATTCAGGTA 
GATCATTTGG ACAAAATGGC ACCCAAAATC ATTCATCTCC GTTGCTTATC TGATGTGGAA 
ATGCTGAAGA ATGGCAATTA TGGGATCTAT ATTACTTCCA GGTCCCTGAA GGCGACTGAT 
CCCAACACAG AGGATGACAA AATATTTTTT AAGATCTTAC AAGGTCCTCG TTATGGCTAC 
CTGGAAAATA TAACAACAGG TGGACTTATC CATGAAGGGT TTAGCCAAAA AGACTTAAAT 
AGCAAAATTA TATTTTATGT CATCAATCCA CTGTGGGAAG TGCATTCAGA CAACTTGGAG 
TTTCAGGTCA CAGACACCAA TGGAAATTCA GCGATTCCCC AAATGCTGGA GCTACGGTGG 
TCTCGCATTG AAATGCAGCA GACCGAATAT GAAGTGTGTG AGAACGTGGG GACGGTGTCC 
CTGAAAATCA CCAGGGCAGG ACACTCTGCT GACTCTGCTT TTATCGCAGT GAAGGAATGT 
CAACCAAGAT GTGGAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_004949102.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004949159.2 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

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CloneID	OGa48009
Clone ID Related Accession (Same CDS sequence)	XM_004949102.2 , XM_004949102.3
Accession Version	XM_004949102.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764317.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004949102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TTGAGAAAAC ATGGCTGTTG CTATTGCTGC CTGTTCTCCT AAGAGTTGTC 
TGCTCCTCTT TTGTAAGTGT CAACCTTGGC ATTAAAGTGA TGAAGGGACA GTCTGTTTTC 
CTGTCAGAAG ATGACCTCAA GTTTTCCATC CCCAGAGAGA AGGATGTGTG CAAAGTCGAA 
GTTGTGAGAA ATGAGCCAAT AACGCAGAGG GTTGGGAAGC TAACTCCACA GGTTTTTGAC 
TGTCACTTTC GCCTTAATGA AGTCAAGTAT ACCCACAACG GTTGTCCGAT CTTAAATGCA 
GACACAGTCA TGCTTAGGCT GTACAGGTTT ACAGAAAATG AAACATTTGT AGAGACATTC 
ACCCTCCACG TACAGTTACT TGAGCCAGAC TGTAACATCA TCAAAATGCG CTCCCGAGCT 
TTGGAGGTGG CTGAGTACTA CGGCCTGTCC AGGGCAATTG ACAAGAACAG CCTCACTTTT 
GACTACAGCA GGAAGATGAA TTTGGAATGC ACCATTTCCA TAGCCTCTTC AGAAACCCAC 
CTCCCTGCTC ACGGCCAGCT CATCATGGGA GACACGCGGC AGGAAAAGCT TCGAGGGGAT 
CAACCACAGA GCTTCCTCCT TGGCCCTAAG CACAGCCCAG GCCAGCAGTG CAGAAATGGG 
AGCTGTGTGT TGGGACTGAG AGTCATCCAT AACACAAAAA TGAGCTGTGA AGAGTTTCTG 
AGGAAGGGCA TTCGGTATCA GCACCTTGAC CCTCGCTCAC CGGATATCGA TTATATCCCA 
GTGAGGCTGG ATCTCACAGA CAGCAGAAGC AAAACTCTGC ATAAGACTGA GTATGCGTGG 
CTCCCTGTTC GGATTAAAAG TGCTATGCCC AATCAAATAC CCAAAGCTGT TCCAATGACA 
AAGTTCATCT TGGAAGTAGA CCAATTTATT TTAACTCCCA TTACAACCAC AACGGTTGAT 
GCTGAAGACA AGGAAACTCC AAAGTCCCTG CTGGTGTTCA ACATTACAAA GCCACCTCTG 
CAGGGCTTCA TTACTCACCT GTCGGACCAC ACAAAGCCTG TTGGTTCCTT CACATGGAAA 
GACCTCAGTG AAATGCTTAT TGCTTATCAG CCTCCAAACA ACAGCCACAC AGAGAGGAGG 
AATTATGAGC TGGAGGTTGA GGTTCATGAC TTCTACTTTG AGAGGAGTTT ACCAACCATT 
GTGCAACTTT CTATTAGAAC AGCAGACACA AATGCTCCTC GGGTTTCATG GAATACAGGC 
CTCAGCCTCC TGGAAGGGCA ATCCCGGCCT ATCACATGGC AACACCTACA GGTGGTAGAC 
AATGATGATA TCCAAAATGT TCGCTTGGTC ACAGTTGATG GCTTGCAGCA TGGGCGGCTC 
ACAGTGAGAG GAGGAAAAGG GTTCATGTTT ACAGTTTCTG ACATTCAGAC TGGAGTTGTT 
CGCTACCATC ACGATGACAG CGATTCCACC AAGGATTTTG TGGTATTCAG GATTTTCGAT 
GGCCTGCATA GTATTCGCCA TAAGTTTCCA ATCAATATCC TCCCTAAAGA TGACAGTCCT 
CCATTTCTTA TCAGCAATGT GGTAATTGAA GTACAGGAGG GACAGACGAT CCTGATCCAG 
GGCTCCATGC TTCAAGCTTC TGACATGGAT TCCAGTGATG ACTATATAGT ATTCAATATT 
ACCAAGCCAC CACAGGCTGG GGAAATCATG AAGAAACCAG GGCCAAACTT AATAGGGTAT 
CCTGTCAGCA GTTTTCTTCA GAGGGATCTG TTTAATGGAA TAATGTATTA TCATCATTTG 
GGAGGAGAAG TATTTGAAGA TTCTATTGAG TTTGTGCTGT GTGATAGCCA TGACCCTCCC 
AATCTCTCGG AGCCACAGGT GATGACGGTG CACATTTCAC CTGTGGATGA TCAGCTGCCC 
AAAGAAGCCC CAGGAGTGAC CCGCCACCTG GTTGTTAAGG AGACTGAGAT TGCTCACCTA 
ACTAAGAAAC ATCTCCACTT CATAGATGTG GAAGAGCAAG ATAGGGAACT CACGTACACC 
ATAACCACTT CCCCATTTTT CTCTTGTGTG CACGGTCACT CAGACGCTGG GAAGCTGTTT 
ATGGTGGACA CCATCCCAAA ACTGGTCAAG GATCCTGCTG CTCTTGCTCT GAGGTCGTTT 
ACTCAGCATG CTGTGAACTA TTTGAAAGTT GCTTACATGC CCCCACTGCA GGACATTGGG 
CCCAATCCTC AACACGTCCA GTTTGTATTT TCTGTCAGCA ATCAGCATGG TGGCACTTTG 
TATGGAATCT GCTTCAATAT CACAATTCTT CCTGTGGACA ACCAAGCCCC AGAGGTTCTT 
ACAAGCCACT TGAGGGTGGA AGAGGGCGGC CTGAGCCCTG TCACGGAGGG ACACATTCTT 
GTCTCTGACG TGGATACAAA ACAGGAACAT CTCCTCCTCC TTCTGCAGAG ACAGCCTCAG 
CATGGGGCAG TGGAGCTGGA TGGTGTTCCC ATGGAGGAGG GCGACAAGCT TTCCTGCGCT 
GATCTGCGTA CCCTGGCAGT CAGTTATCGG CATGATGGTT CTGAGACTCT TACAGATGAA 
GTCCTTTTTG TAGCCACAGA TGGCATCCAC TCCTTAGAGT TCACCCTGCA GGTCAAGGTG 
TTGCCCATGA ATGATGAGCC ACCTGTTATG CAGACAGGGC TTATCAATGT GTTAAGCTGC 
CCGGAGGGTG AAGAAGTGGT CCTCTCCTCG GAGTACATAT ATGCCACAGA TGCAGACAGT 
GATGACATGA AGCTGGTGTT TGTGCTGGTT CGCCAGCCCT CCCAGGGGGT GCTGAGGAGA 
GCCGGCATTG CTGTGGAGCG TTTCTCCCAG GCTGATGTCA TCTCTGGCTT AGTCACCTAT 
AAGCACACTG GTGGAGAGAT TGGCTTGACT CTTGCCTTTG AGACCTTAAC TTTTGTTGTT 
TCTGATGATG AAGCCGGCCC AGATGTGAAA GACTGCTGTT ATGATAGACC TCTTCCTCCT 
CCAGTTCCTC TTCACCGTCC ACTCCCAGTG TACGACCTTA ACATCACTGT ACTTCCAGTG 
GACAACCAAC CTCCATCCAT TGCAATTGGT GCAAGGTTTG TTGTGGAGGA GGGCTCCTCA 
GCAGCCCTTA CCTCTAACCA TTTGTTTGCC ACCGACCCTG ACACCCCTGC GGATGACTTA 
GAGTTTGTCT TGGTTTCTCC TCCTCAGTTT GGTTATATTG AAAATATACT GCCTTCTCCT 
GGGTTTGAGA AGAGCAACGT TGGTATAAGT ATAGCTTCGT TTCAGTTGAA GCACCTGAAA 
GCCCTGAATA TAAACTATGT ACAAGCCAGG CACATGCGAA TGGAGCCAAG AGCAGACCAC 
TTTGTACTTT ATGTCACCGA TGGGCTGCAT CGTTCAGCAG AGATTCCATT TTTTGTGCTG 
ATCAACCCAA CCAATGATGA AGTCCCAGAA TTCATAGCAA GAAACATTAC TGTTCAAGAA 
GGTATGATGA AAGAGCTGGA TCTCTCTGTT ATCAGTGCTG TTGATCTGGA TGTGCCTCAA 
GACCAGTTGG TATTTGGTGT TGTGCAGCAG CCTTCACATG GATTCCTCAT TAACGGAGTT 
CATGGCAATG ATATTCTACA CTACAAAGAG TTAATTCAAC ACCACAACCA TGGGTTGCTT 
GTTCATGACT TTTCTATGGA GCTACTGCAG AATGGTATGA AACTGATGTA CATGCATGAC 
GATTCAGAAA GCCTCAGTGA CAGCTTTAAA ATCCAGCTAT CAGATGGGAA ACATAAAGTC 
CTCAGAACCA TTTCAGTAAA GGTCATCCCA GTTAATGATG AGAAACCAGT GCTGAGCAAG 
AAGGATAATA TAGAGGTGCA TATGGGTGAA ACTCGAATTA TTTCTAGTGC TGTTCTGTCA 
GCTGAAGATA AAGATACCCC TAGGGAGAGA ATTTACTACT TATTTGAAAG ACTTCCAGAA 
AGTGGCCAGC TTCAACTCAA GGTAGGCAGA GACTGGGTGA CTCTCCAAAC CGGAATGAAG 
TGTACTCAGG AAGAACTGGA TATGAACCTT GTGAGATACG TCCACACAGG AGCTATAGGG 
TCCAAGAACC AAGACTTGTT CACATTTTAT CTGTGGGATG GGTACAACAG GTCCCCAGCT 
GTAGACTTCT ACATAAATAT CAAGGACATG GAAAAGGGAG ACATTGCTGT TTTCATAAAA 
CCCCTCAAGG TATCAAAAGG GGATCGAGGC TACATAACAA CTGATGTCCT CCTTTCACTG 
GATGGTACTG ACAAGCCTGA AGAGCTTCTC TATGTGATAA CTTCCCCACC TCAGTATGGA 
CAAATAGAGT ATGCTAGCTA TCCTGACATC CCCATAACGA GCTTCAGTCA AATGGATGTA 
GCAAGTCAGA TAGTCTGCTA TGTTCACAAC ACCAAGGCAC ATTCAAGGCA AGATGCATTC 
AGATTCATCA TCAGCAATGG ACTGAGGACT AAACATGGGA CATTCATGAT CTCCTTGGAG 
GCAGTTGACC AAACGTTGCC TACACTATCT AGGAACAGGG GGTTAAGATT AGTAGAAGGC 
AATATGGTAC TCCTTTCTCC TGATGTCCTG CAGCTTACAG ACTCTGACAC TGCCAAAGAA 
AACCTTACCT TCATCTTGGC TCAGCTCCCC CAGTATGGTC ATCTCTATTA CCGAGGGGCT 
GTACTACTGC AGTACAGTTT CACCCAACGT GATGTGGACA ATATGGATGT TGCGTATAAG 
CACAGAGGTG GGGATTCTCG GGTTGACAAA TTTACATTTG TTGCAACAGA TAGGACTAAT 
CAAGGCTTCA TCGTGAATGG GAGGGTGCAG ATCGAGCCAG TGGCATTCAC CATTCAGGTA 
GATCATTTGG ACAAAATGGC ACCCAAAATC ATTCATCTCC GTTGCTTATC TGATGTGGAA 
ATGCTGAAGA ATGGCAATTA TGGGATCTAT ATTACTTCCA GGTCCCTGAA GGCGACTGAT 
CCCAACACAG AGGATGACAA AATATTTTTT AAGATCTTAC AAGGTCCTCG TTATGGCTAC 
CTGGAAAATA TAACAACAGG TGGACTTATC CATGAAGGGT TTAGCCAAAA AGACTTAAAT 
AGCAAAATTA TATTTTATGT CATCAATCCA CTGTGGGAAG TGCATTCAGA CAACTTGGAG 
TTTCAGGTCA CAGACACCAA TGGAAATTCA GCGATTCCCC AAATGCTGGA GCTACGGTGG 
TCTCGCATTG AAATGCAGCA GACCGAATAT GAAGTGTGTG AGAACGTGGG GACGGTGTCC 
CTGAAAATCA CCAGGGCAGG ACACTCTGCT GACTCTGCTT TTATCGCAGT GAAGGTCAAT 
GAAATATCTG CTGTGCTGGG AAAGGACTTT ACAGTGACTC CCTCCAAACT TATTCAGTTT 
GATCCAGGAA TGTCAACCAA GATGTGGAAT ATAGCAATTA CTTATGACAG ATTAGAGGAA 
GATGATGAAG TCTTTGAAGT AGTTCTGAAC TCCTCTGTGA ATGCTGTTCT TGGCACCAAG 
ACAAAAGCTG TAGTGAAAAT TCTGGACTCA AAAGGAGGTC AGTGCAGTAC TTTACATTCT 
TCCAGCCAAA GCAAGCAAGA CTTCTGGAGG AGAGGCACAC TGTTTGCAGT TTCCTCAGGC 
TCCTCATCAC CTCCCAGGCC TGGCAATGTT CACTTGGAAG GGATCTCACC ACCTCCTTCC 
AAAAGGATGA GAGAACACAG AGGTGACACA CTGCAGGACC GCAGGTTGGC GTATCAGTCA 
AGGGCCAAAC TGAGAGTGAC TGGAAATGGC AGAATAGTTC ATCCTTCATC TATATTTAGA 
AATGGAACAG ATGTGGTTTA CAAATACCAT GGGATGGTAT CACTCAAAAT AGAGGGTGAC 
ACCTCATCAG CTAAAGCAAA CAAGAAGGCT GAAGTGTCAG TAATTAACCA AGCACAACCA 
CAGATAAATG CCATTTCCAC TAGGAAGACA GAAATCCCAC AAGCTGATAA GGCAGAACTG 
GCATCTGAAG CAAGCTCAAG ACGTCAGGAC TTTCACTCCT TTTCAAGGGC CTGTACACCA 
GATTTGAAGG GGCTCATGCA TTATGAAGAA AGCACTCAGA AGTTATTTCA GTGTGACGGG 
ATAGAATGGA AATTTTGGAG TTCCCAAAGC AAGGAGGCAA GCATGAAAAA ATGTCCCTCT 
GGATGGCGTC ATCGTGATGG CAGCTGCTAT TTCCTGGTAG TGAATCAAAA GGTCACCTGG 
GATACTGCTG CTCAGGCCTG CAGAGAACAA TACCTGGGGA GTCTCACAAG TGTGGCTAAT 
GAACAACACA TGCAGTGGCT GTGGGACTTC GGCGGGAGGA AGCCCTTTTG GATAGGCTTG 
AATGACCAAG TCAATCCTGG ACACTGGGAG TGGAACGGAG GAGAACCTGT TACCTACATA 
AACTGGAGAA GAGGCTCCTA TCGCTTTACA AAGAGAGGCA ACAACTGTGT AGTGGTGCAG 
AAGAGAGGAA AATGGCAAGC AACTGACTGC AGGAAGGCCA AATGGAACAG CTACATATGT 
TCAAGAAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004949159.2
CDS	725..7279
Translation	MKPFEKTWLLLLLPVLLRVVCSSFVSVNLGIKVMKGQSVFLSEDDLKFSIPREKDVCKVEVVRNEPITQRVGKLTPQVFDCHFRLNEVKYTHNGCPILNADTVMLRLYRFTENETFVETFTLHVQLLEPDCNIIKMRSRALEVAEYYGLSRAIDKNSLTFDYSRKMNLECTISIASSETHLPAHGQLIMGDTRQEKLRGDQPQSFLLGPKHSPGQQCRNGSCVLGLRVIHNTKMSCEEFLRKGIRYQHLDPRSPDIDYIPVRLDLTDSRSKTLHKTEYAWLPVRIKSAMPNQIPKAVPMTKFILEVDQFILTPITTTTVDAEDKETPKSLLVFNITKPPLQGFITHLSDHTKPVGSFTWKDLSEMLIAYQPPNNSHTERRNYELEVEVHDFYFERSLPTIVQLSIRTADTNAPRVSWNTGLSLLEGQSRPITWQHLQVVDNDDIQNVRLVTVDGLQHGRLTVRGGKGFMFTVSDIQTGVVRYHHDDSDSTKDFVVFRIFDGLHSIRHKFPINILPKDDSPPFLISNVVIEVQEGQTILIQGSMLQASDMDSSDDYIVFNITKPPQAGEIMKKPGPNLIGYPVSSFLQRDLFNGIMYYHHLGGEVFEDSIEFVLCDSHDPPNLSEPQVMTVHISPVDDQLPKEAPGVTRHLVVKETEIAHLTKKHLHFIDVEEQDRELTYTITTSPFFSCVHGHSDAGKLFMVDTIPKLVKDPAALALRSFTQHAVNYLKVAYMPPLQDIGPNPQHVQFVFSVSNQHGGTLYGICFNITILPVDNQAPEVLTSHLRVEEGGLSPVTEGHILVSDVDTKQEHLLLLLQRQPQHGAVELDGVPMEEGDKLSCADLRTLAVSYRHDGSETLTDEVLFVATDGIHSLEFTLQVKVLPMNDEPPVMQTGLINVLSCPEGEEVVLSSEYIYATDADSDDMKLVFVLVRQPSQGVLRRAGIAVERFSQADVISGLVTYKHTGGEIGLTLAFETLTFVVSDDEAGPDVKDCCYDRPLPPPVPLHRPLPVYDLNITVLPVDNQPPSIAIGARFVVEEGSSAALTSNHLFATDPDTPADDLEFVLVSPPQFGYIENILPSPGFEKSNVGISIASFQLKHLKALNINYVQARHMRMEPRADHFVLYVTDGLHRSAEIPFFVLINPTNDEVPEFIARNITVQEGMMKELDLSVISAVDLDVPQDQLVFGVVQQPSHGFLINGVHGNDILHYKELIQHHNHGLLVHDFSMELLQNGMKLMYMHDDSESLSDSFKIQLSDGKHKVLRTISVKVIPVNDEKPVLSKKDNIEVHMGETRIISSAVLSAEDKDTPRERIYYLFERLPESGQLQLKVGRDWVTLQTGMKCTQEELDMNLVRYVHTGAIGSKNQDLFTFYLWDGYNRSPAVDFYINIKDMEKGDIAVFIKPLKVSKGDRGYITTDVLLSLDGTDKPEELLYVITSPPQYGQIEYASYPDIPITSFSQMDVASQIVCYVHNTKAHSRQDAFRFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKVNEISAVLGKDFTVTPSKLIQFDPGMSTKMWNIAITYDRLEEDDEVFEVVLNSSVNAVLGTKTKAVVKILDSKGGQCSTLHSSSQSKQDFWRRGTLFAVSSGSSSPPRPGNVHLEGISPPPSKRMREHRGDTLQDRRLAYQSRAKLRVTGNGRIVHPSSIFRNGTDVVYKYHGMVSLKIEGDTSSAKANKKAEVSVINQAQPQINAISTRKTEIPQADKAELASEASSRRQDFHSFSRACTPDLKGLMHYEESTQKLFQCDGIEWKFWSSQSKEASMKKCPSGWRHRDGSCYFLVVNQKVTWDTAAQACREQYLGSLTSVANEQHMQWLWDFGGRKPFWIGLNDQVNPGHWEWNGGEPVTYINWRRGSYRFTKRGNNCVVVQKRGKWQATDCRKAKWNSYICSRKL

Target ORF information:

RefSeq Version	XM_004949102.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004949102.2

ORF Insert Sequence:

ATGAAGCCCT TTGAGAAAAC ATGGCTGTTG CTATTGCTGC CTGTTCTCCT AAGAGTTGTC 
TGCTCCTCTT TTGTAAGTGT CAACCTTGGC ATTAAAGTGA TGAAGGGACA GTCTGTTTTC 
CTGTCAGAAG ATGACCTCAA GTTTTCCATC CCCAGAGAGA AGGATGTGTG CAAAGTCGAA 
GTTGTGAGAA ATGAGCCAAT AACGCAGAGG GTTGGGAAGC TAACTCCACA GGTTTTTGAC 
TGTCACTTTC GCCTTAATGA AGTCAAGTAT ACCCACAACG GTTGTCCGAT CTTAAATGCA 
GACACAGTCA TGCTTAGGCT GTACAGGTTT ACAGAAAATG AAACATTTGT AGAGACATTC 
ACCCTCCACG TACAGTTACT TGAGCCAGAC TGTAACATCA TCAAAATGCG CTCCCGAGCT 
TTGGAGGTGG CTGAGTACTA CGGCCTGTCC AGGGCAATTG ACAAGAACAG CCTCACTTTT 
GACTACAGCA GGAAGATGAA TTTGGAATGC ACCATTTCCA TAGCCTCTTC AGAAACCCAC 
CTCCCTGCTC ACGGCCAGCT CATCATGGGA GACACGCGGC AGGAAAAGCT TCGAGGGGAT 
CAACCACAGA GCTTCCTCCT TGGCCCTAAG CACAGCCCAG GCCAGCAGTG CAGAAATGGG 
AGCTGTGTGT TGGGACTGAG AGTCATCCAT AACACAAAAA TGAGCTGTGA AGAGTTTCTG 
AGGAAGGGCA TTCGGTATCA GCACCTTGAC CCTCGCTCAC CGGATATCGA TTATATCCCA 
GTGAGGCTGG ATCTCACAGA CAGCAGAAGC AAAACTCTGC ATAAGACTGA GTATGCGTGG 
CTCCCTGTTC GGATTAAAAG TGCTATGCCC AATCAAATAC CCAAAGCTGT TCCAATGACA 
AAGTTCATCT TGGAAGTAGA CCAATTTATT TTAACTCCCA TTACAACCAC AACGGTTGAT 
GCTGAAGACA AGGAAACTCC AAAGTCCCTG CTGGTGTTCA ACATTACAAA GCCACCTCTG 
CAGGGCTTCA TTACTCACCT GTCGGACCAC ACAAAGCCTG TTGGTTCCTT CACATGGAAA 
GACCTCAGTG AAATGCTTAT TGCTTATCAG CCTCCAAACA ACAGCCACAC AGAGAGGAGG 
AATTATGAGC TGGAGGTTGA GGTTCATGAC TTCTACTTTG AGAGGAGTTT ACCAACCATT 
GTGCAACTTT CTATTAGAAC AGCAGACACA AATGCTCCTC GGGTTTCATG GAATACAGGC 
CTCAGCCTCC TGGAAGGGCA ATCCCGGCCT ATCACATGGC AACACCTACA GGTGGTAGAC 
AATGATGATA TCCAAAATGT TCGCTTGGTC ACAGTTGATG GCTTGCAGCA TGGGCGGCTC 
ACAGTGAGAG GAGGAAAAGG GTTCATGTTT ACAGTTTCTG ACATTCAGAC TGGAGTTGTT 
CGCTACCATC ACGATGACAG CGATTCCACC AAGGATTTTG TGGTATTCAG GATTTTCGAT 
GGCCTGCATA GTATTCGCCA TAAGTTTCCA ATCAATATCC TCCCTAAAGA TGACAGTCCT 
CCATTTCTTA TCAGCAATGT GGTAATTGAA GTACAGGAGG GACAGACGAT CCTGATCCAG 
GGCTCCATGC TTCAAGCTTC TGACATGGAT TCCAGTGATG ACTATATAGT ATTCAATATT 
ACCAAGCCAC CACAGGCTGG GGAAATCATG AAGAAACCAG GGCCAAACTT AATAGGGTAT 
CCTGTCAGCA GTTTTCTTCA GAGGGATCTG TTTAATGGAA TAATGTATTA TCATCATTTG 
GGAGGAGAAG TATTTGAAGA TTCTATTGAG TTTGTGCTGT GTGATAGCCA TGACCCTCCC 
AATCTCTCGG AGCCACAGGT GATGACGGTG CACATTTCAC CTGTGGATGA TCAGCTGCCC 
AAAGAAGCCC CAGGAGTGAC CCGCCACCTG GTTGTTAAGG AGACTGAGAT TGCTCACCTA 
ACTAAGAAAC ATCTCCACTT CATAGATGTG GAAGAGCAAG ATAGGGAACT CACGTACACC 
ATAACCACTT CCCCATTTTT CTCTTGTGTG CACGGTCACT CAGACGCTGG GAAGCTGTTT 
ATGGTGGACA CCATCCCAAA ACTGGTCAAG GATCCTGCTG CTCTTGCTCT GAGGTCGTTT 
ACTCAGCATG CTGTGAACTA TTTGAAAGTT GCTTACATGC CCCCACTGCA GGACATTGGG 
CCCAATCCTC AACACGTCCA GTTTGTATTT TCTGTCAGCA ATCAGCATGG TGGCACTTTG 
TATGGAATCT GCTTCAATAT CACAATTCTT CCTGTGGACA ACCAAGCCCC AGAGGTTCTT 
ACAAGCCACT TGAGGGTGGA AGAGGGCGGC CTGAGCCCTG TCACGGAGGG ACACATTCTT 
GTCTCTGACG TGGATACAAA ACAGGAACAT CTCCTCCTCC TTCTGCAGAG ACAGCCTCAG 
CATGGGGCAG TGGAGCTGGA TGGTGTTCCC ATGGAGGAGG GCGACAAGCT TTCCTGCGCT 
GATCTGCGTA CCCTGGCAGT CAGTTATCGG CATGATGGTT CTGAGACTCT TACAGATGAA 
GTCCTTTTTG TAGCCACAGA TGGCATCCAC TCCTTAGAGT TCACCCTGCA GGTCAAGGTG 
TTGCCCATGA ATGATGAGCC ACCTGTTATG CAGACAGGGC TTATCAATGT GTTAAGCTGC 
CCGGAGGGTG AAGAAGTGGT CCTCTCCTCG GAGTACATAT ATGCCACAGA TGCAGACAGT 
GATGACATGA AGCTGGTGTT TGTGCTGGTT CGCCAGCCCT CCCAGGGGGT GCTGAGGAGA 
GCCGGCATTG CTGTGGAGCG TTTCTCCCAG GCTGATGTCA TCTCTGGCTT AGTCACCTAT 
AAGCACACTG GTGGAGAGAT TGGCTTGACT CTTGCCTTTG AGACCTTAAC TTTTGTTGTT 
TCTGATGATG AAGCCGGCCC AGATGTGAAA GACTGCTGTT ATGATAGACC TCTTCCTCCT 
CCAGTTCCTC TTCACCGTCC ACTCCCAGTG TACGACCTTA ACATCACTGT ACTTCCAGTG 
GACAACCAAC CTCCATCCAT TGCAATTGGT GCAAGGTTTG TTGTGGAGGA GGGCTCCTCA 
GCAGCCCTTA CCTCTAACCA TTTGTTTGCC ACCGACCCTG ACACCCCTGC GGATGACTTA 
GAGTTTGTCT TGGTTTCTCC TCCTCAGTTT GGTTATATTG AAAATATACT GCCTTCTCCT 
GGGTTTGAGA AGAGCAACGT TGGTATAAGT ATAGCTTCGT TTCAGTTGAA GCACCTGAAA 
GCCCTGAATA TAAACTATGT ACAAGCCAGG CACATGCGAA TGGAGCCAAG AGCAGACCAC 
TTTGTACTTT ATGTCACCGA TGGGCTGCAT CGTTCAGCAG AGATTCCATT TTTTGTGCTG 
ATCAACCCAA CCAATGATGA AGTCCCAGAA TTCATAGCAA GAAACATTAC TGTTCAAGAA 
GGTATGATGA AAGAGCTGGA TCTCTCTGTT ATCAGTGCTG TTGATCTGGA TGTGCCTCAA 
GACCAGTTGG TATTTGGTGT TGTGCAGCAG CCTTCACATG GATTCCTCAT TAACGGAGTT 
CATGGCAATG ATATTCTACA CTACAAAGAG TTAATTCAAC ACCACAACCA TGGGTTGCTT 
GTTCATGACT TTTCTATGGA GCTACTGCAG AATGGTATGA AACTGATGTA CATGCATGAC 
GATTCAGAAA GCCTCAGTGA CAGCTTTAAA ATCCAGCTAT CAGATGGGAA ACATAAAGTC 
CTCAGAACCA TTTCAGTAAA GGTCATCCCA GTTAATGATG AGAAACCAGT GCTGAGCAAG 
AAGGATAATA TAGAGGTGCA TATGGGTGAA ACTCGAATTA TTTCTAGTGC TGTTCTGTCA 
GCTGAAGATA AAGATACCCC TAGGGAGAGA ATTTACTACT TATTTGAAAG ACTTCCAGAA 
AGTGGCCAGC TTCAACTCAA GGTAGGCAGA GACTGGGTGA CTCTCCAAAC CGGAATGAAG 
TGTACTCAGG AAGAACTGGA TATGAACCTT GTGAGATACG TCCACACAGG AGCTATAGGG 
TCCAAGAACC AAGACTTGTT CACATTTTAT CTGTGGGATG GGTACAACAG GTCCCCAGCT 
GTAGACTTCT ACATAAATAT CAAGGACATG GAAAAGGGAG ACATTGCTGT TTTCATAAAA 
CCCCTCAAGG TATCAAAAGG GGATCGAGGC TACATAACAA CTGATGTCCT CCTTTCACTG 
GATGGTACTG ACAAGCCTGA AGAGCTTCTC TATGTGATAA CTTCCCCACC TCAGTATGGA 
CAAATAGAGT ATGCTAGCTA TCCTGACATC CCCATAACGA GCTTCAGTCA AATGGATGTA 
GCAAGTCAGA TAGTCTGCTA TGTTCACAAC ACCAAGGCAC ATTCAAGGCA AGATGCATTC 
AGATTCATCA TCAGCAATGG ACTGAGGACT AAACATGGGA CATTCATGAT CTCCTTGGAG 
GCAGTTGACC AAACGTTGCC TACACTATCT AGGAACAGGG GGTTAAGATT AGTAGAAGGC 
AATATGGTAC TCCTTTCTCC TGATGTCCTG CAGCTTACAG ACTCTGACAC TGCCAAAGAA 
AACCTTACCT TCATCTTGGC TCAGCTCCCC CAGTATGGTC ATCTCTATTA CCGAGGGGCT 
GTACTACTGC AGTACAGTTT CACCCAACGT GATGTGGACA ATATGGATGT TGCGTATAAG 
CACAGAGGTG GGGATTCTCG GGTTGACAAA TTTACATTTG TTGCAACAGA TAGGACTAAT 
CAAGGCTTCA TCGTGAATGG GAGGGTGCAG ATCGAGCCAG TGGCATTCAC CATTCAGGTA 
GATCATTTGG ACAAAATGGC ACCCAAAATC ATTCATCTCC GTTGCTTATC TGATGTGGAA 
ATGCTGAAGA ATGGCAATTA TGGGATCTAT ATTACTTCCA GGTCCCTGAA GGCGACTGAT 
CCCAACACAG AGGATGACAA AATATTTTTT AAGATCTTAC AAGGTCCTCG TTATGGCTAC 
CTGGAAAATA TAACAACAGG TGGACTTATC CATGAAGGGT TTAGCCAAAA AGACTTAAAT 
AGCAAAATTA TATTTTATGT CATCAATCCA CTGTGGGAAG TGCATTCAGA CAACTTGGAG 
TTTCAGGTCA CAGACACCAA TGGAAATTCA GCGATTCCCC AAATGCTGGA GCTACGGTGG 
TCTCGCATTG AAATGCAGCA GACCGAATAT GAAGTGTGTG AGAACGTGGG GACGGTGTCC 
CTGAAAATCA CCAGGGCAGG ACACTCTGCT GACTCTGCTT TTATCGCAGT GAAGGTCAAT 
GAAATATCTG CTGTGCTGGG AAAGGACTTT ACAGTGACTC CCTCCAAACT TATTCAGTTT 
GATCCAGGAA TGTCAACCAA GATGTGGAAT ATAGCAATTA CTTATGACAG ATTAGAGGAA 
GATGATGAAG TCTTTGAAGT AGTTCTGAAC TCCTCTGTGA ATGCTGTTCT TGGCACCAAG 
ACAAAAGCTG TAGTGAAAAT TCTGGACTCA AAAGGAGGTC AGTGCAGTAC TTTACATTCT 
TCCAGCCAAA GCAAGCAAGA CTTCTGGAGG AGAGGCACAC TGTTTGCAGT TTCCTCAGGC 
TCCTCATCAC CTCCCAGGCC TGGCAATGTT CACTTGGAAG GGATCTCACC ACCTCCTTCC 
AAAAGGATGA GAGAACACAG AGGTGACACA CTGCAGGACC GCAGGTTGGC GTATCAGTCA 
AGGGCCAAAC TGAGAGTGAC TGGAAATGGC AGAATAGTTC ATCCTTCATC TATATTTAGA 
AATGGAACAG ATGTGGTTTA CAAATACCAT GGGATGGTAT CACTCAAAAT AGAGGGTGAC 
ACCTCATCAG CTAAAGCAAA CAAGAAGGCT GAAGTGTCAG TAATTAACCA AGCACAACCA 
CAGATAAATG CCATTTCCAC TAGGAAGACA GAAATCCCAC AAGCTGATAA GGCAGAACTG 
GCATCTGAAG CAAGCTCAAG ACGTCAGGAC TTTCACTCCT TTTCAAGGGC CTGTACACCA 
GATTTGAAGG GGCTCATGCA TTATGAAGAA AGCACTCAGA AGTTATTTCA GTGTGACGGG 
ATAGAATGGA AATTTTGGAG TTCCCAAAGC AAGGAGGCAA GCATGAAAAA ATGTCCCTCT 
GGATGGCGTC ATCGTGATGG CAGCTGCTAT TTCCTGGTAG TGAATCAAAA GGTCACCTGG 
GATACTGCTG CTCAGGCCTG CAGAGAACAA TACCTGGGGA GTCTCACAAG TGTGGCTAAT 
GAACAACACA TGCAGTGGCT GTGGGACTTC GGCGGGAGGA AGCCCTTTTG GATAGGCTTG 
AATGACCAAG TCAATCCTGG ACACTGGGAG TGGAACGGAG GAGAACCTGT TACCTACATA 
AACTGGAGAA GAGGCTCCTA TCGCTTTACA AAGAGAGGCA ACAACTGTGT AGTGGTGCAG 
AAGAGAGGAA AATGGCAAGC AACTGACTGC AGGAAGGCCA AATGGAACAG CTACATATGT 
TCAAGAAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_004949102.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004949159.2 Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

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CloneID	OGa48009
Clone ID Related Accession (Same CDS sequence)	XM_004949102.2 , XM_004949102.3
Accession Version	XM_004949102.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004949102.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TTGAGAAAAC ATGGCTGTTG CTATTGCTGC CTGTTCTCCT AAGAGTTGTC 
TGCTCCTCTT TTGTAAGTGT CAACCTTGGC ATTAAAGTGA TGAAGGGACA GTCTGTTTTC 
CTGTCAGAAG ATGACCTCAA GTTTTCCATC CCCAGAGAGA AGGATGTGTG CAAAGTCGAA 
GTTGTGAGAA ATGAGCCAAT AACGCAGAGG GTTGGGAAGC TAACTCCACA GGTTTTTGAC 
TGTCACTTTC GCCTTAATGA AGTCAAGTAT ACCCACAACG GTTGTCCGAT CTTAAATGCA 
GACACAGTCA TGCTTAGGCT GTACAGGTTT ACAGAAAATG AAACATTTGT AGAGACATTC 
ACCCTCCACG TACAGTTACT TGAGCCAGAC TGTAACATCA TCAAAATGCG CTCCCGAGCT 
TTGGAGGTGG CTGAGTACTA CGGCCTGTCC AGGGCAATTG ACAAGAACAG CCTCACTTTT 
GACTACAGCA GGAAGATGAA TTTGGAATGC ACCATTTCCA TAGCCTCTTC AGAAACCCAC 
CTCCCTGCTC ACGGCCAGCT CATCATGGGA GACACGCGGC AGGAAAAGCT TCGAGGGGAT 
CAACCACAGA GCTTCCTCCT TGGCCCTAAG CACAGCCCAG GCCAGCAGTG CAGAAATGGG 
AGCTGTGTGT TGGGACTGAG AGTCATCCAT AACACAAAAA TGAGCTGTGA AGAGTTTCTG 
AGGAAGGGCA TTCGGTATCA GCACCTTGAC CCTCGCTCAC CGGATATCGA TTATATCCCA 
GTGAGGCTGG ATCTCACAGA CAGCAGAAGC AAAACTCTGC ATAAGACTGA GTATGCGTGG 
CTCCCTGTTC GGATTAAAAG TGCTATGCCC AATCAAATAC CCAAAGCTGT TCCAATGACA 
AAGTTCATCT TGGAAGTAGA CCAATTTATT TTAACTCCCA TTACAACCAC AACGGTTGAT 
GCTGAAGACA AGGAAACTCC AAAGTCCCTG CTGGTGTTCA ACATTACAAA GCCACCTCTG 
CAGGGCTTCA TTACTCACCT GTCGGACCAC ACAAAGCCTG TTGGTTCCTT CACATGGAAA 
GACCTCAGTG AAATGCTTAT TGCTTATCAG CCTCCAAACA ACAGCCACAC AGAGAGGAGG 
AATTATGAGC TGGAGGTTGA GGTTCATGAC TTCTACTTTG AGAGGAGTTT ACCAACCATT 
GTGCAACTTT CTATTAGAAC AGCAGACACA AATGCTCCTC GGGTTTCATG GAATACAGGC 
CTCAGCCTCC TGGAAGGGCA ATCCCGGCCT ATCACATGGC AACACCTACA GGTGGTAGAC 
AATGATGATA TCCAAAATGT TCGCTTGGTC ACAGTTGATG GCTTGCAGCA TGGGCGGCTC 
ACAGTGAGAG GAGGAAAAGG GTTCATGTTT ACAGTTTCTG ACATTCAGAC TGGAGTTGTT 
CGCTACCATC ACGATGACAG CGATTCCACC AAGGATTTTG TGGTATTCAG GATTTTCGAT 
GGCCTGCATA GTATTCGCCA TAAGTTTCCA ATCAATATCC TCCCTAAAGA TGACAGTCCT 
CCATTTCTTA TCAGCAATGT GGTAATTGAA GTACAGGAGG GACAGACGAT CCTGATCCAG 
GGCTCCATGC TTCAAGCTTC TGACATGGAT TCCAGTGATG ACTATATAGT ATTCAATATT 
ACCAAGCCAC CACAGGCTGG GGAAATCATG AAGAAACCAG GGCCAAACTT AATAGGGTAT 
CCTGTCAGCA GTTTTCTTCA GAGGGATCTG TTTAATGGAA TAATGTATTA TCATCATTTG 
GGAGGAGAAG TATTTGAAGA TTCTATTGAG TTTGTGCTGT GTGATAGCCA TGACCCTCCC 
AATCTCTCGG AGCCACAGGT GATGACGGTG CACATTTCAC CTGTGGATGA TCAGCTGCCC 
AAAGAAGCCC CAGGAGTGAC CCGCCACCTG GTTGTTAAGG AGACTGAGAT TGCTCACCTA 
ACTAAGAAAC ATCTCCACTT CATAGATGTG GAAGAGCAAG ATAGGGAACT CACGTACACC 
ATAACCACTT CCCCATTTTT CTCTTGTGTG CACGGTCACT CAGACGCTGG GAAGCTGTTT 
ATGGTGGACA CCATCCCAAA ACTGGTCAAG GATCCTGCTG CTCTTGCTCT GAGGTCGTTT 
ACTCAGCATG CTGTGAACTA TTTGAAAGTT GCTTACATGC CCCCACTGCA GGACATTGGG 
CCCAATCCTC AACACGTCCA GTTTGTATTT TCTGTCAGCA ATCAGCATGG TGGCACTTTG 
TATGGAATCT GCTTCAATAT CACAATTCTT CCTGTGGACA ACCAAGCCCC AGAGGTTCTT 
ACAAGCCACT TGAGGGTGGA AGAGGGCGGC CTGAGCCCTG TCACGGAGGG ACACATTCTT 
GTCTCTGACG TGGATACAAA ACAGGAACAT CTCCTCCTCC TTCTGCAGAG ACAGCCTCAG 
CATGGGGCAG TGGAGCTGGA TGGTGTTCCC ATGGAGGAGG GCGACAAGCT TTCCTGCGCT 
GATCTGCGTA CCCTGGCAGT CAGTTATCGG CATGATGGTT CTGAGACTCT TACAGATGAA 
GTCCTTTTTG TAGCCACAGA TGGCATCCAC TCCTTAGAGT TCACCCTGCA GGTCAAGGTG 
TTGCCCATGA ATGATGAGCC ACCTGTTATG CAGACAGGGC TTATCAATGT GTTAAGCTGC 
CCGGAGGGTG AAGAAGTGGT CCTCTCCTCG GAGTACATAT ATGCCACAGA TGCAGACAGT 
GATGACATGA AGCTGGTGTT TGTGCTGGTT CGCCAGCCCT CCCAGGGGGT GCTGAGGAGA 
GCCGGCATTG CTGTGGAGCG TTTCTCCCAG GCTGATGTCA TCTCTGGCTT AGTCACCTAT 
AAGCACACTG GTGGAGAGAT TGGCTTGACT CTTGCCTTTG AGACCTTAAC TTTTGTTGTT 
TCTGATGATG AAGCCGGCCC AGATGTGAAA GACTGCTGTT ATGATAGACC TCTTCCTCCT 
CCAGTTCCTC TTCACCGTCC ACTCCCAGTG TACGACCTTA ACATCACTGT ACTTCCAGTG 
GACAACCAAC CTCCATCCAT TGCAATTGGT GCAAGGTTTG TTGTGGAGGA GGGCTCCTCA 
GCAGCCCTTA CCTCTAACCA TTTGTTTGCC ACCGACCCTG ACACCCCTGC GGATGACTTA 
GAGTTTGTCT TGGTTTCTCC TCCTCAGTTT GGTTATATTG AAAATATACT GCCTTCTCCT 
GGGTTTGAGA AGAGCAACGT TGGTATAAGT ATAGCTTCGT TTCAGTTGAA GCACCTGAAA 
GCCCTGAATA TAAACTATGT ACAAGCCAGG CACATGCGAA TGGAGCCAAG AGCAGACCAC 
TTTGTACTTT ATGTCACCGA TGGGCTGCAT CGTTCAGCAG AGATTCCATT TTTTGTGCTG 
ATCAACCCAA CCAATGATGA AGTCCCAGAA TTCATAGCAA GAAACATTAC TGTTCAAGAA 
GGTATGATGA AAGAGCTGGA TCTCTCTGTT ATCAGTGCTG TTGATCTGGA TGTGCCTCAA 
GACCAGTTGG TATTTGGTGT TGTGCAGCAG CCTTCACATG GATTCCTCAT TAACGGAGTT 
CATGGCAATG ATATTCTACA CTACAAAGAG TTAATTCAAC ACCACAACCA TGGGTTGCTT 
GTTCATGACT TTTCTATGGA GCTACTGCAG AATGGTATGA AACTGATGTA CATGCATGAC 
GATTCAGAAA GCCTCAGTGA CAGCTTTAAA ATCCAGCTAT CAGATGGGAA ACATAAAGTC 
CTCAGAACCA TTTCAGTAAA GGTCATCCCA GTTAATGATG AGAAACCAGT GCTGAGCAAG 
AAGGATAATA TAGAGGTGCA TATGGGTGAA ACTCGAATTA TTTCTAGTGC TGTTCTGTCA 
GCTGAAGATA AAGATACCCC TAGGGAGAGA ATTTACTACT TATTTGAAAG ACTTCCAGAA 
AGTGGCCAGC TTCAACTCAA GGTAGGCAGA GACTGGGTGA CTCTCCAAAC CGGAATGAAG 
TGTACTCAGG AAGAACTGGA TATGAACCTT GTGAGATACG TCCACACAGG AGCTATAGGG 
TCCAAGAACC AAGACTTGTT CACATTTTAT CTGTGGGATG GGTACAACAG GTCCCCAGCT 
GTAGACTTCT ACATAAATAT CAAGGACATG GAAAAGGGAG ACATTGCTGT TTTCATAAAA 
CCCCTCAAGG TATCAAAAGG GGATCGAGGC TACATAACAA CTGATGTCCT CCTTTCACTG 
GATGGTACTG ACAAGCCTGA AGAGCTTCTC TATGTGATAA CTTCCCCACC TCAGTATGGA 
CAAATAGAGT ATGCTAGCTA TCCTGACATC CCCATAACGA GCTTCAGTCA AATGGATGTA 
GCAAGTCAGA TAGTCTGCTA TGTTCACAAC ACCAAGGCAC ATTCAAGGCA AGATGCATTC 
AGATTCATCA TCAGCAATGG ACTGAGGACT AAACATGGGA CATTCATGAT CTCCTTGGAG 
GCAGTTGACC AAACGTTGCC TACACTATCT AGGAACAGGG GGTTAAGATT AGTAGAAGGC 
AATATGGTAC TCCTTTCTCC TGATGTCCTG CAGCTTACAG ACTCTGACAC TGCCAAAGAA 
AACCTTACCT TCATCTTGGC TCAGCTCCCC CAGTATGGTC ATCTCTATTA CCGAGGGGCT 
GTACTACTGC AGTACAGTTT CACCCAACGT GATGTGGACA ATATGGATGT TGCGTATAAG 
CACAGAGGTG GGGATTCTCG GGTTGACAAA TTTACATTTG TTGCAACAGA TAGGACTAAT 
CAAGGCTTCA TCGTGAATGG GAGGGTGCAG ATCGAGCCAG TGGCATTCAC CATTCAGGTA 
GATCATTTGG ACAAAATGGC ACCCAAAATC ATTCATCTCC GTTGCTTATC TGATGTGGAA 
ATGCTGAAGA ATGGCAATTA TGGGATCTAT ATTACTTCCA GGTCCCTGAA GGCGACTGAT 
CCCAACACAG AGGATGACAA AATATTTTTT AAGATCTTAC AAGGTCCTCG TTATGGCTAC 
CTGGAAAATA TAACAACAGG TGGACTTATC CATGAAGGGT TTAGCCAAAA AGACTTAAAT 
AGCAAAATTA TATTTTATGT CATCAATCCA CTGTGGGAAG TGCATTCAGA CAACTTGGAG 
TTTCAGGTCA CAGACACCAA TGGAAATTCA GCGATTCCCC AAATGCTGGA GCTACGGTGG 
TCTCGCATTG AAATGCAGCA GACCGAATAT GAAGTGTGTG AGAACGTGGG GACGGTGTCC 
CTGAAAATCA CCAGGGCAGG ACACTCTGCT GACTCTGCTT TTATCGCAGT GAAGGTCAAT 
GAAATATCTG CTGTGCTGGG AAAGGACTTT ACAGTGACTC CCTCCAAACT TATTCAGTTT 
GATCCAGGAA TGTCAACCAA GATGTGGAAT ATAGCAATTA CTTATGACAG ATTAGAGGAA 
GATGATGAAG TCTTTGAAGT AGTTCTGAAC TCCTCTGTGA ATGCTGTTCT TGGCACCAAG 
ACAAAAGCTG TAGTGAAAAT TCTGGACTCA AAAGGAGGTC AGTGCAGTAC TTTACATTCT 
TCCAGCCAAA GCAAGCAAGA CTTCTGGAGG AGAGGCACAC TGTTTGCAGT TTCCTCAGGC 
TCCTCATCAC CTCCCAGGCC TGGCAATGTT CACTTGGAAG GGATCTCACC ACCTCCTTCC 
AAAAGGATGA GAGAACACAG AGGTGACACA CTGCAGGACC GCAGGTTGGC GTATCAGTCA 
AGGGCCAAAC TGAGAGTGAC TGGAAATGGC AGAATAGTTC ATCCTTCATC TATATTTAGA 
AATGGAACAG ATGTGGTTTA CAAATACCAT GGGATGGTAT CACTCAAAAT AGAGGGTGAC 
ACCTCATCAG CTAAAGCAAA CAAGAAGGCT GAAGTGTCAG TAATTAACCA AGCACAACCA 
CAGATAAATG CCATTTCCAC TAGGAAGACA GAAATCCCAC AAGCTGATAA GGCAGAACTG 
GCATCTGAAG CAAGCTCAAG ACGTCAGGAC TTTCACTCCT TTTCAAGGGC CTGTACACCA 
GATTTGAAGG GGCTCATGCA TTATGAAGAA AGCACTCAGA AGTTATTTCA GTGTGACGGG 
ATAGAATGGA AATTTTGGAG TTCCCAAAGC AAGGAGGCAA GCATGAAAAA ATGTCCCTCT 
GGATGGCGTC ATCGTGATGG CAGCTGCTAT TTCCTGGTAG TGAATCAAAA GGTCACCTGG 
GATACTGCTG CTCAGGCCTG CAGAGAACAA TACCTGGGGA GTCTCACAAG TGTGGCTAAT 
GAACAACACA TGCAGTGGCT GTGGGACTTC GGCGGGAGGA AGCCCTTTTG GATAGGCTTG 
AATGACCAAG TCAATCCTGG ACACTGGGAG TGGAACGGAG GAGAACCTGT TACCTACATA 
AACTGGAGAA GAGGCTCCTA TCGCTTTACA AAGAGAGGCA ACAACTGTGT AGTGGTGCAG 
AAGAGAGGAA AATGGCAAGC AACTGACTGC AGGAAGGCCA AATGGAACAG CTACATATGT 
TCAAGAAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004949159.2
CDS	712..7266
Translation	MKPFEKTWLLLLLPVLLRVVCSSFVSVNLGIKVMKGQSVFLSEDDLKFSIPREKDVCKVEVVRNEPITQRVGKLTPQVFDCHFRLNEVKYTHNGCPILNADTVMLRLYRFTENETFVETFTLHVQLLEPDCNIIKMRSRALEVAEYYGLSRAIDKNSLTFDYSRKMNLECTISIASSETHLPAHGQLIMGDTRQEKLRGDQPQSFLLGPKHSPGQQCRNGSCVLGLRVIHNTKMSCEEFLRKGIRYQHLDPRSPDIDYIPVRLDLTDSRSKTLHKTEYAWLPVRIKSAMPNQIPKAVPMTKFILEVDQFILTPITTTTVDAEDKETPKSLLVFNITKPPLQGFITHLSDHTKPVGSFTWKDLSEMLIAYQPPNNSHTERRNYELEVEVHDFYFERSLPTIVQLSIRTADTNAPRVSWNTGLSLLEGQSRPITWQHLQVVDNDDIQNVRLVTVDGLQHGRLTVRGGKGFMFTVSDIQTGVVRYHHDDSDSTKDFVVFRIFDGLHSIRHKFPINILPKDDSPPFLISNVVIEVQEGQTILIQGSMLQASDMDSSDDYIVFNITKPPQAGEIMKKPGPNLIGYPVSSFLQRDLFNGIMYYHHLGGEVFEDSIEFVLCDSHDPPNLSEPQVMTVHISPVDDQLPKEAPGVTRHLVVKETEIAHLTKKHLHFIDVEEQDRELTYTITTSPFFSCVHGHSDAGKLFMVDTIPKLVKDPAALALRSFTQHAVNYLKVAYMPPLQDIGPNPQHVQFVFSVSNQHGGTLYGICFNITILPVDNQAPEVLTSHLRVEEGGLSPVTEGHILVSDVDTKQEHLLLLLQRQPQHGAVELDGVPMEEGDKLSCADLRTLAVSYRHDGSETLTDEVLFVATDGIHSLEFTLQVKVLPMNDEPPVMQTGLINVLSCPEGEEVVLSSEYIYATDADSDDMKLVFVLVRQPSQGVLRRAGIAVERFSQADVISGLVTYKHTGGEIGLTLAFETLTFVVSDDEAGPDVKDCCYDRPLPPPVPLHRPLPVYDLNITVLPVDNQPPSIAIGARFVVEEGSSAALTSNHLFATDPDTPADDLEFVLVSPPQFGYIENILPSPGFEKSNVGISIASFQLKHLKALNINYVQARHMRMEPRADHFVLYVTDGLHRSAEIPFFVLINPTNDEVPEFIARNITVQEGMMKELDLSVISAVDLDVPQDQLVFGVVQQPSHGFLINGVHGNDILHYKELIQHHNHGLLVHDFSMELLQNGMKLMYMHDDSESLSDSFKIQLSDGKHKVLRTISVKVIPVNDEKPVLSKKDNIEVHMGETRIISSAVLSAEDKDTPRERIYYLFERLPESGQLQLKVGRDWVTLQTGMKCTQEELDMNLVRYVHTGAIGSKNQDLFTFYLWDGYNRSPAVDFYINIKDMEKGDIAVFIKPLKVSKGDRGYITTDVLLSLDGTDKPEELLYVITSPPQYGQIEYASYPDIPITSFSQMDVASQIVCYVHNTKAHSRQDAFRFIISNGLRTKHGTFMISLEAVDQTLPTLSRNRGLRLVEGNMVLLSPDVLQLTDSDTAKENLTFILAQLPQYGHLYYRGAVLLQYSFTQRDVDNMDVAYKHRGGDSRVDKFTFVATDRTNQGFIVNGRVQIEPVAFTIQVDHLDKMAPKIIHLRCLSDVEMLKNGNYGIYITSRSLKATDPNTEDDKIFFKILQGPRYGYLENITTGGLIHEGFSQKDLNSKIIFYVINPLWEVHSDNLEFQVTDTNGNSAIPQMLELRWSRIEMQQTEYEVCENVGTVSLKITRAGHSADSAFIAVKVNEISAVLGKDFTVTPSKLIQFDPGMSTKMWNIAITYDRLEEDDEVFEVVLNSSVNAVLGTKTKAVVKILDSKGGQCSTLHSSSQSKQDFWRRGTLFAVSSGSSSPPRPGNVHLEGISPPPSKRMREHRGDTLQDRRLAYQSRAKLRVTGNGRIVHPSSIFRNGTDVVYKYHGMVSLKIEGDTSSAKANKKAEVSVINQAQPQINAISTRKTEIPQADKAELASEASSRRQDFHSFSRACTPDLKGLMHYEESTQKLFQCDGIEWKFWSSQSKEASMKKCPSGWRHRDGSCYFLVVNQKVTWDTAAQACREQYLGSLTSVANEQHMQWLWDFGGRKPFWIGLNDQVNPGHWEWNGGEPVTYINWRRGSYRFTKRGNNCVVVQKRGKWQATDCRKAKWNSYICSRKL

Target ORF information:

RefSeq Version	XM_004949102.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004949102.3

ORF Insert Sequence:

ATGAAGCCCT TTGAGAAAAC ATGGCTGTTG CTATTGCTGC CTGTTCTCCT AAGAGTTGTC 
TGCTCCTCTT TTGTAAGTGT CAACCTTGGC ATTAAAGTGA TGAAGGGACA GTCTGTTTTC 
CTGTCAGAAG ATGACCTCAA GTTTTCCATC CCCAGAGAGA AGGATGTGTG CAAAGTCGAA 
GTTGTGAGAA ATGAGCCAAT AACGCAGAGG GTTGGGAAGC TAACTCCACA GGTTTTTGAC 
TGTCACTTTC GCCTTAATGA AGTCAAGTAT ACCCACAACG GTTGTCCGAT CTTAAATGCA 
GACACAGTCA TGCTTAGGCT GTACAGGTTT ACAGAAAATG AAACATTTGT AGAGACATTC 
ACCCTCCACG TACAGTTACT TGAGCCAGAC TGTAACATCA TCAAAATGCG CTCCCGAGCT 
TTGGAGGTGG CTGAGTACTA CGGCCTGTCC AGGGCAATTG ACAAGAACAG CCTCACTTTT 
GACTACAGCA GGAAGATGAA TTTGGAATGC ACCATTTCCA TAGCCTCTTC AGAAACCCAC 
CTCCCTGCTC ACGGCCAGCT CATCATGGGA GACACGCGGC AGGAAAAGCT TCGAGGGGAT 
CAACCACAGA GCTTCCTCCT TGGCCCTAAG CACAGCCCAG GCCAGCAGTG CAGAAATGGG 
AGCTGTGTGT TGGGACTGAG AGTCATCCAT AACACAAAAA TGAGCTGTGA AGAGTTTCTG 
AGGAAGGGCA TTCGGTATCA GCACCTTGAC CCTCGCTCAC CGGATATCGA TTATATCCCA 
GTGAGGCTGG ATCTCACAGA CAGCAGAAGC AAAACTCTGC ATAAGACTGA GTATGCGTGG 
CTCCCTGTTC GGATTAAAAG TGCTATGCCC AATCAAATAC CCAAAGCTGT TCCAATGACA 
AAGTTCATCT TGGAAGTAGA CCAATTTATT TTAACTCCCA TTACAACCAC AACGGTTGAT 
GCTGAAGACA AGGAAACTCC AAAGTCCCTG CTGGTGTTCA ACATTACAAA GCCACCTCTG 
CAGGGCTTCA TTACTCACCT GTCGGACCAC ACAAAGCCTG TTGGTTCCTT CACATGGAAA 
GACCTCAGTG AAATGCTTAT TGCTTATCAG CCTCCAAACA ACAGCCACAC AGAGAGGAGG 
AATTATGAGC TGGAGGTTGA GGTTCATGAC TTCTACTTTG AGAGGAGTTT ACCAACCATT 
GTGCAACTTT CTATTAGAAC AGCAGACACA AATGCTCCTC GGGTTTCATG GAATACAGGC 
CTCAGCCTCC TGGAAGGGCA ATCCCGGCCT ATCACATGGC AACACCTACA GGTGGTAGAC 
AATGATGATA TCCAAAATGT TCGCTTGGTC ACAGTTGATG GCTTGCAGCA TGGGCGGCTC 
ACAGTGAGAG GAGGAAAAGG GTTCATGTTT ACAGTTTCTG ACATTCAGAC TGGAGTTGTT 
CGCTACCATC ACGATGACAG CGATTCCACC AAGGATTTTG TGGTATTCAG GATTTTCGAT 
GGCCTGCATA GTATTCGCCA TAAGTTTCCA ATCAATATCC TCCCTAAAGA TGACAGTCCT 
CCATTTCTTA TCAGCAATGT GGTAATTGAA GTACAGGAGG GACAGACGAT CCTGATCCAG 
GGCTCCATGC TTCAAGCTTC TGACATGGAT TCCAGTGATG ACTATATAGT ATTCAATATT 
ACCAAGCCAC CACAGGCTGG GGAAATCATG AAGAAACCAG GGCCAAACTT AATAGGGTAT 
CCTGTCAGCA GTTTTCTTCA GAGGGATCTG TTTAATGGAA TAATGTATTA TCATCATTTG 
GGAGGAGAAG TATTTGAAGA TTCTATTGAG TTTGTGCTGT GTGATAGCCA TGACCCTCCC 
AATCTCTCGG AGCCACAGGT GATGACGGTG CACATTTCAC CTGTGGATGA TCAGCTGCCC 
AAAGAAGCCC CAGGAGTGAC CCGCCACCTG GTTGTTAAGG AGACTGAGAT TGCTCACCTA 
ACTAAGAAAC ATCTCCACTT CATAGATGTG GAAGAGCAAG ATAGGGAACT CACGTACACC 
ATAACCACTT CCCCATTTTT CTCTTGTGTG CACGGTCACT CAGACGCTGG GAAGCTGTTT 
ATGGTGGACA CCATCCCAAA ACTGGTCAAG GATCCTGCTG CTCTTGCTCT GAGGTCGTTT 
ACTCAGCATG CTGTGAACTA TTTGAAAGTT GCTTACATGC CCCCACTGCA GGACATTGGG 
CCCAATCCTC AACACGTCCA GTTTGTATTT TCTGTCAGCA ATCAGCATGG TGGCACTTTG 
TATGGAATCT GCTTCAATAT CACAATTCTT CCTGTGGACA ACCAAGCCCC AGAGGTTCTT 
ACAAGCCACT TGAGGGTGGA AGAGGGCGGC CTGAGCCCTG TCACGGAGGG ACACATTCTT 
GTCTCTGACG TGGATACAAA ACAGGAACAT CTCCTCCTCC TTCTGCAGAG ACAGCCTCAG 
CATGGGGCAG TGGAGCTGGA TGGTGTTCCC ATGGAGGAGG GCGACAAGCT TTCCTGCGCT 
GATCTGCGTA CCCTGGCAGT CAGTTATCGG CATGATGGTT CTGAGACTCT TACAGATGAA 
GTCCTTTTTG TAGCCACAGA TGGCATCCAC TCCTTAGAGT TCACCCTGCA GGTCAAGGTG 
TTGCCCATGA ATGATGAGCC ACCTGTTATG CAGACAGGGC TTATCAATGT GTTAAGCTGC 
CCGGAGGGTG AAGAAGTGGT CCTCTCCTCG GAGTACATAT ATGCCACAGA TGCAGACAGT 
GATGACATGA AGCTGGTGTT TGTGCTGGTT CGCCAGCCCT CCCAGGGGGT GCTGAGGAGA 
GCCGGCATTG CTGTGGAGCG TTTCTCCCAG GCTGATGTCA TCTCTGGCTT AGTCACCTAT 
AAGCACACTG GTGGAGAGAT TGGCTTGACT CTTGCCTTTG AGACCTTAAC TTTTGTTGTT 
TCTGATGATG AAGCCGGCCC AGATGTGAAA GACTGCTGTT ATGATAGACC TCTTCCTCCT 
CCAGTTCCTC TTCACCGTCC ACTCCCAGTG TACGACCTTA ACATCACTGT ACTTCCAGTG 
GACAACCAAC CTCCATCCAT TGCAATTGGT GCAAGGTTTG TTGTGGAGGA GGGCTCCTCA 
GCAGCCCTTA CCTCTAACCA TTTGTTTGCC ACCGACCCTG ACACCCCTGC GGATGACTTA 
GAGTTTGTCT TGGTTTCTCC TCCTCAGTTT GGTTATATTG AAAATATACT GCCTTCTCCT 
GGGTTTGAGA AGAGCAACGT TGGTATAAGT ATAGCTTCGT TTCAGTTGAA GCACCTGAAA 
GCCCTGAATA TAAACTATGT ACAAGCCAGG CACATGCGAA TGGAGCCAAG AGCAGACCAC 
TTTGTACTTT ATGTCACCGA TGGGCTGCAT CGTTCAGCAG AGATTCCATT TTTTGTGCTG 
ATCAACCCAA CCAATGATGA AGTCCCAGAA TTCATAGCAA GAAACATTAC TGTTCAAGAA 
GGTATGATGA AAGAGCTGGA TCTCTCTGTT ATCAGTGCTG TTGATCTGGA TGTGCCTCAA 
GACCAGTTGG TATTTGGTGT TGTGCAGCAG CCTTCACATG GATTCCTCAT TAACGGAGTT 
CATGGCAATG ATATTCTACA CTACAAAGAG TTAATTCAAC ACCACAACCA TGGGTTGCTT 
GTTCATGACT TTTCTATGGA GCTACTGCAG AATGGTATGA AACTGATGTA CATGCATGAC 
GATTCAGAAA GCCTCAGTGA CAGCTTTAAA ATCCAGCTAT CAGATGGGAA ACATAAAGTC 
CTCAGAACCA TTTCAGTAAA GGTCATCCCA GTTAATGATG AGAAACCAGT GCTGAGCAAG 
AAGGATAATA TAGAGGTGCA TATGGGTGAA ACTCGAATTA TTTCTAGTGC TGTTCTGTCA 
GCTGAAGATA AAGATACCCC TAGGGAGAGA ATTTACTACT TATTTGAAAG ACTTCCAGAA 
AGTGGCCAGC TTCAACTCAA GGTAGGCAGA GACTGGGTGA CTCTCCAAAC CGGAATGAAG 
TGTACTCAGG AAGAACTGGA TATGAACCTT GTGAGATACG TCCACACAGG AGCTATAGGG 
TCCAAGAACC AAGACTTGTT CACATTTTAT CTGTGGGATG GGTACAACAG GTCCCCAGCT 
GTAGACTTCT ACATAAATAT CAAGGACATG GAAAAGGGAG ACATTGCTGT TTTCATAAAA 
CCCCTCAAGG TATCAAAAGG GGATCGAGGC TACATAACAA CTGATGTCCT CCTTTCACTG 
GATGGTACTG ACAAGCCTGA AGAGCTTCTC TATGTGATAA CTTCCCCACC TCAGTATGGA 
CAAATAGAGT ATGCTAGCTA TCCTGACATC CCCATAACGA GCTTCAGTCA AATGGATGTA 
GCAAGTCAGA TAGTCTGCTA TGTTCACAAC ACCAAGGCAC ATTCAAGGCA AGATGCATTC 
AGATTCATCA TCAGCAATGG ACTGAGGACT AAACATGGGA CATTCATGAT CTCCTTGGAG 
GCAGTTGACC AAACGTTGCC TACACTATCT AGGAACAGGG GGTTAAGATT AGTAGAAGGC 
AATATGGTAC TCCTTTCTCC TGATGTCCTG CAGCTTACAG ACTCTGACAC TGCCAAAGAA 
AACCTTACCT TCATCTTGGC TCAGCTCCCC CAGTATGGTC ATCTCTATTA CCGAGGGGCT 
GTACTACTGC AGTACAGTTT CACCCAACGT GATGTGGACA ATATGGATGT TGCGTATAAG 
CACAGAGGTG GGGATTCTCG GGTTGACAAA TTTACATTTG TTGCAACAGA TAGGACTAAT 
CAAGGCTTCA TCGTGAATGG GAGGGTGCAG ATCGAGCCAG TGGCATTCAC CATTCAGGTA 
GATCATTTGG ACAAAATGGC ACCCAAAATC ATTCATCTCC GTTGCTTATC TGATGTGGAA 
ATGCTGAAGA ATGGCAATTA TGGGATCTAT ATTACTTCCA GGTCCCTGAA GGCGACTGAT 
CCCAACACAG AGGATGACAA AATATTTTTT AAGATCTTAC AAGGTCCTCG TTATGGCTAC 
CTGGAAAATA TAACAACAGG TGGACTTATC CATGAAGGGT TTAGCCAAAA AGACTTAAAT 
AGCAAAATTA TATTTTATGT CATCAATCCA CTGTGGGAAG TGCATTCAGA CAACTTGGAG 
TTTCAGGTCA CAGACACCAA TGGAAATTCA GCGATTCCCC AAATGCTGGA GCTACGGTGG 
TCTCGCATTG AAATGCAGCA GACCGAATAT GAAGTGTGTG AGAACGTGGG GACGGTGTCC 
CTGAAAATCA CCAGGGCAGG ACACTCTGCT GACTCTGCTT TTATCGCAGT GAAGGTCAAT 
GAAATATCTG CTGTGCTGGG AAAGGACTTT ACAGTGACTC CCTCCAAACT TATTCAGTTT 
GATCCAGGAA TGTCAACCAA GATGTGGAAT ATAGCAATTA CTTATGACAG ATTAGAGGAA 
GATGATGAAG TCTTTGAAGT AGTTCTGAAC TCCTCTGTGA ATGCTGTTCT TGGCACCAAG 
ACAAAAGCTG TAGTGAAAAT TCTGGACTCA AAAGGAGGTC AGTGCAGTAC TTTACATTCT 
TCCAGCCAAA GCAAGCAAGA CTTCTGGAGG AGAGGCACAC TGTTTGCAGT TTCCTCAGGC 
TCCTCATCAC CTCCCAGGCC TGGCAATGTT CACTTGGAAG GGATCTCACC ACCTCCTTCC 
AAAAGGATGA GAGAACACAG AGGTGACACA CTGCAGGACC GCAGGTTGGC GTATCAGTCA 
AGGGCCAAAC TGAGAGTGAC TGGAAATGGC AGAATAGTTC ATCCTTCATC TATATTTAGA 
AATGGAACAG ATGTGGTTTA CAAATACCAT GGGATGGTAT CACTCAAAAT AGAGGGTGAC 
ACCTCATCAG CTAAAGCAAA CAAGAAGGCT GAAGTGTCAG TAATTAACCA AGCACAACCA 
CAGATAAATG CCATTTCCAC TAGGAAGACA GAAATCCCAC AAGCTGATAA GGCAGAACTG 
GCATCTGAAG CAAGCTCAAG ACGTCAGGAC TTTCACTCCT TTTCAAGGGC CTGTACACCA 
GATTTGAAGG GGCTCATGCA TTATGAAGAA AGCACTCAGA AGTTATTTCA GTGTGACGGG 
ATAGAATGGA AATTTTGGAG TTCCCAAAGC AAGGAGGCAA GCATGAAAAA ATGTCCCTCT 
GGATGGCGTC ATCGTGATGG CAGCTGCTAT TTCCTGGTAG TGAATCAAAA GGTCACCTGG 
GATACTGCTG CTCAGGCCTG CAGAGAACAA TACCTGGGGA GTCTCACAAG TGTGGCTAAT 
GAACAACACA TGCAGTGGCT GTGGGACTTC GGCGGGAGGA AGCCCTTTTG GATAGGCTTG 
AATGACCAAG TCAATCCTGG ACACTGGGAG TGGAACGGAG GAGAACCTGT TACCTACATA 
AACTGGAGAA GAGGCTCCTA TCGCTTTACA AAGAGAGGCA ACAACTGTGT AGTGGTGCAG 
AAGAGAGGAA AATGGCAAGC AACTGACTGC AGGAAGGCCA AATGGAACAG CTACATATGT 
TCAAGAAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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FREM1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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