LRRC8B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRC8B
Entrez Gene ID	424516
Full Name	leucine rich repeat containing 8 family member B
General protein information	Preferred Names leucine rich repeat containing 8 family member B Names volume-regulated anion channel subunit LRRC8B
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004936638.2	XP_004936695.1	volume-regulated anion channel subunit LRRC8B
XM_015290645.1	XP_015146131.1	volume-regulated anion channel subunit LRRC8B

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8B	NP_056165.1
Rattus norvegicus (Norway rat)	Lrrc8b	NP_001100674.1
Gallus gallus (chicken)	LRRC8B	XP_004936694.1
	LRRC8B	XP_002801670.1
Pan troglodytes (chimpanzee)	LRRC8B	XP_001150409.1
Bos taurus (cattle)	LRRC8B	XP_005204398.1
Mus musculus (house mouse)	Lrrc8b	NP_001028722.1
Canis lupus familiaris (dog)	LRRC8B	XP_005622010.1
Xenopus tropicalis (tropical clawed frog)	lrrc8b	XP_002941172.1

Gene Ontology

The following LRRC8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa11944	XM_004936638.2 Latest version!	Gallus gallus leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OGa11944	XM_015290645.1 Latest version!	Gallus gallus leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRC8B ( XM_004936638.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004936695.1 Gallus gallus leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa11944
Clone ID Related Accession (Same CDS sequence)	XM_004936638.2 , XM_015290645.1
Accession Version	XM_004936638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	volume-regulated anion channel subunit LRRC8B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004936638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACGC TAACAGAACT CAAATACTTA GCAGATGCTC AGTCATCCTA CCACATACTA 
AAACCATGGT GGGATGTCTT CTGGTATTAT CTCACCATGA TAATGCTGCT AGTTGCTGTG 
CTTGCTGGGG CTCTCCAGCT TACTCAAACC AGAGTGTTGT GTTGTCTTCC TTGCAAGCTA 
GAATTTGACA ATCATTGTGC CGTGCCTTGG GATCTAATTA AAACCAATCT TAACATGTCT 
GCTAGCTCTA CTGCACAGAT AATCATCCCA CTTAAAATCC AGAATGATCT CCATCGGCAG 
CAGTATTCCT ATATTGATGC AGTATGCTAT GAGAGACAGC TTCATTGGTT TGCCAAATTT 
TTTCCATACC TAGTGCTTCT GCATACCTTT ATTTTTGCAG CTTGCAGTAA TTTCTGGCTT 
TACTATCCTA GTACAAGTTC CAGGCTTGAG CACTTTGTAG CAATTCTTCA GAAGTGTTTT 
GATTCTCCAT GGACAACACG TGCCCTTTCA GAAACAGTTG CTGAGCAGTC TGTGAGGAAA 
TTGCCAGTTG CCAAATCAAA AGCAGTGATT TCATCGCCTG GGAGTTCAGT AGATGTAGGT 
GCCAGCAGAC AGTCCCTGCC ATATACACAA CCTGGCTTGG AAACCACCGG AAGAGAAAAT 
TCAAGTGTTC TTGATAAAAA AGAAGGAGAA CAAGCTAAAG CTATATTTGA AAAAGTGAAG 
AAATTCAGAG TGCATGTTGA AGAGAAGGAT GTCATATATA GAGTGTATAT GAAACAGATC 
ATAGTCAAAG TCATTGTGTT TGTAATAATA ATAATTTATG TTCCCTATTA TTTATCCTTT 
ATTAAACTTG AAATTGATTG CATAGTTAAT GTACAAGCCT TTACAGGCTA CAAAAGGTAC 
CAGTGTGTCT ATTCATTAGC AGAAATTTTT AAAGTTCTGG CTTCATTTTA TGTTGTTTTG 
GTGATCTTCT ATGGCTTAAC TTGTACGTAT AGTTTGTGGT GGATGTTAAG AAGCTCACTT 
AAGCAATATT CTTTTGAGAA GTTGAGAGAG AAAAGTAACT ACAGCGATAT ACCTGATGTA 
AAGAATGACT TTGCATTTAT TCTCCACTTG GCAGATCAGT ATGATCCTCT TTATTCCCAG 
CGATTCTCAA TATTCTTGTC TGATTTGAGT GAAAATAAAC TGAAACAGAT AAATCTGAAC 
AATGAATGGT CAGTGGAAAA ACTGAAAAAT AAGCTAATAA GAAATTCCCA GGACAAGGTT 
GAACTTCACC TTTTCATGTT AAGTGGTCTT CCAGACAGTG TCTTTGAACT GACAGAAATA 
GAAGTCCTAA GCTTGGAACT TATTCCTGAA GCAAAGCTTC CTTCAGCTGT GACCCAGCTA 
GTCAATCTCA AAGATCTCAA CGTTTATCAT TCATCAATAA CTATGGATTA TCCAGCAGTG 
AGCTTTTTAG AAGAAAATCT GAAAACATTG CGTCTGAAAT CTAGTGAGAT GGGAAGAATT 
CCATTTTGGG TATTTCATCT GAAAAACCTC AAAGAATTGT GCTTAACAGG ATATTTCATG 
CTAGATCATC AGAACTCCAT TTACAATGAA AGCTTTCAGG GGCTAAAAAA TCTGAGATCA 
ATTCACTTAA AAAACAACCT TTCTCGCATA CCTCAAGTGG TTACAGATCT GCCTTCTTTG 
CAGCACTTAT CTATCAACAA CGAGGGAAAT AAACTCATAG TGCTAAATAA CTTGAAGAAG 
CTTGTGAACT TGAGAACCTT GGAATTAATC TGCTGTGATC TAGAGCGCAT TCCCCATTCC 
ATTTTTACCC TAAACAACTT GCATGAAATT GACTTAAGAG AAAATAATCT CAGAACAGTG 
GAAGAAATCA TCAGCTTTCA ACACCTTAAG AACTTGTCTT GCTTAAAGTT GTGGCACAAC 
AGCATTTCAT ACGTCCCAGT GCAAATTGGT GCGTTGTCAA ACCTGGAACA ATTGTATCTA 
AATTACAATA ATATTAAAGA TGTTCCATTG CAGCTGTTTC TTTGCAAAAA GTTACAATAT 
TTGGATCTTA GCTATAATAA GCTAACCTCC ATCCCTGAAG AAATCGGTTA TCTGACCAAT 
CTACAGTACT TGGCTTTGAC TAAAAACCAT ATTGAAATGT TGCCTGATAG ATTGTTTCAG 
TGCAAAAAGC TGCAATTTCT TCTTCTGGGA AATAACAGTC TGATGAATTT GTCCCCTCTT 
GTGGGCCAGC TACTCAATCT TGTTCACTTA GAGCTCACTG GAAACTATCT TGAATCACTT 
CCTGCTGAAC TGGAAGAATG TCAGTTCCTA AAACGGAATA GTCTAATTGT AGAAGAAAGG 
TTGTTAAAAA CGCTTCCACC TCGTGTAAGA GATCGCCTGC AGACCAGCTC AGATAAGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004936695.1
CDS	317..2719
Translation	MITLTELKYLADAQSSYHILKPWWDVFWYYLTMIMLLVAVLAGALQLTQTRVLCCLPCKLEFDNHCAVPWDLIKTNLNMSASSTAQIIIPLKIQNDLHRQQYSYIDAVCYERQLHWFAKFFPYLVLLHTFIFAACSNFWLYYPSTSSRLEHFVAILQKCFDSPWTTRALSETVAEQSVRKLPVAKSKAVISSPGSSVDVGASRQSLPYTQPGLETTGRENSSVLDKKEGEQAKAIFEKVKKFRVHVEEKDVIYRVYMKQIIVKVIVFVIIIIYVPYYLSFIKLEIDCIVNVQAFTGYKRYQCVYSLAEIFKVLASFYVVLVIFYGLTCTYSLWWMLRSSLKQYSFEKLREKSNYSDIPDVKNDFAFILHLADQYDPLYSQRFSIFLSDLSENKLKQINLNNEWSVEKLKNKLIRNSQDKVELHLFMLSGLPDSVFELTEIEVLSLELIPEAKLPSAVTQLVNLKDLNVYHSSITMDYPAVSFLEENLKTLRLKSSEMGRIPFWVFHLKNLKELCLTGYFMLDHQNSIYNESFQGLKNLRSIHLKNNLSRIPQVVTDLPSLQHLSINNEGNKLIVLNNLKKLVNLRTLELICCDLERIPHSIFTLNNLHEIDLRENNLRTVEEIISFQHLKNLSCLKLWHNSISYVPVQIGALSNLEQLYLNYNNIKDVPLQLFLCKKLQYLDLSYNKLTSIPEEIGYLTNLQYLALTKNHIEMLPDRLFQCKKLQFLLLGNNSLMNLSPLVGQLLNLVHLELTGNYLESLPAELEECQFLKRNSLIVEERLLKTLPPRVRDRLQTSSDKC

Target ORF information:

RefSeq Version	XM_004936638.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004936638.2

ORF Insert Sequence:

ATGATCACGC TAACAGAACT CAAATACTTA GCAGATGCTC AGTCATCCTA CCACATACTA 
AAACCATGGT GGGATGTCTT CTGGTATTAT CTCACCATGA TAATGCTGCT AGTTGCTGTG 
CTTGCTGGGG CTCTCCAGCT TACTCAAACC AGAGTGTTGT GTTGTCTTCC TTGCAAGCTA 
GAATTTGACA ATCATTGTGC CGTGCCTTGG GATCTAATTA AAACCAATCT TAACATGTCT 
GCTAGCTCTA CTGCACAGAT AATCATCCCA CTTAAAATCC AGAATGATCT CCATCGGCAG 
CAGTATTCCT ATATTGATGC AGTATGCTAT GAGAGACAGC TTCATTGGTT TGCCAAATTT 
TTTCCATACC TAGTGCTTCT GCATACCTTT ATTTTTGCAG CTTGCAGTAA TTTCTGGCTT 
TACTATCCTA GTACAAGTTC CAGGCTTGAG CACTTTGTAG CAATTCTTCA GAAGTGTTTT 
GATTCTCCAT GGACAACACG TGCCCTTTCA GAAACAGTTG CTGAGCAGTC TGTGAGGAAA 
TTGCCAGTTG CCAAATCAAA AGCAGTGATT TCATCGCCTG GGAGTTCAGT AGATGTAGGT 
GCCAGCAGAC AGTCCCTGCC ATATACACAA CCTGGCTTGG AAACCACCGG AAGAGAAAAT 
TCAAGTGTTC TTGATAAAAA AGAAGGAGAA CAAGCTAAAG CTATATTTGA AAAAGTGAAG 
AAATTCAGAG TGCATGTTGA AGAGAAGGAT GTCATATATA GAGTGTATAT GAAACAGATC 
ATAGTCAAAG TCATTGTGTT TGTAATAATA ATAATTTATG TTCCCTATTA TTTATCCTTT 
ATTAAACTTG AAATTGATTG CATAGTTAAT GTACAAGCCT TTACAGGCTA CAAAAGGTAC 
CAGTGTGTCT ATTCATTAGC AGAAATTTTT AAAGTTCTGG CTTCATTTTA TGTTGTTTTG 
GTGATCTTCT ATGGCTTAAC TTGTACGTAT AGTTTGTGGT GGATGTTAAG AAGCTCACTT 
AAGCAATATT CTTTTGAGAA GTTGAGAGAG AAAAGTAACT ACAGCGATAT ACCTGATGTA 
AAGAATGACT TTGCATTTAT TCTCCACTTG GCAGATCAGT ATGATCCTCT TTATTCCCAG 
CGATTCTCAA TATTCTTGTC TGATTTGAGT GAAAATAAAC TGAAACAGAT AAATCTGAAC 
AATGAATGGT CAGTGGAAAA ACTGAAAAAT AAGCTAATAA GAAATTCCCA GGACAAGGTT 
GAACTTCACC TTTTCATGTT AAGTGGTCTT CCAGACAGTG TCTTTGAACT GACAGAAATA 
GAAGTCCTAA GCTTGGAACT TATTCCTGAA GCAAAGCTTC CTTCAGCTGT GACCCAGCTA 
GTCAATCTCA AAGATCTCAA CGTTTATCAT TCATCAATAA CTATGGATTA TCCAGCAGTG 
AGCTTTTTAG AAGAAAATCT GAAAACATTG CGTCTGAAAT CTAGTGAGAT GGGAAGAATT 
CCATTTTGGG TATTTCATCT GAAAAACCTC AAAGAATTGT GCTTAACAGG ATATTTCATG 
CTAGATCATC AGAACTCCAT TTACAATGAA AGCTTTCAGG GGCTAAAAAA TCTGAGATCA 
ATTCACTTAA AAAACAACCT TTCTCGCATA CCTCAAGTGG TTACAGATCT GCCTTCTTTG 
CAGCACTTAT CTATCAACAA CGAGGGAAAT AAACTCATAG TGCTAAATAA CTTGAAGAAG 
CTTGTGAACT TGAGAACCTT GGAATTAATC TGCTGTGATC TAGAGCGCAT TCCCCATTCC 
ATTTTTACCC TAAACAACTT GCATGAAATT GACTTAAGAG AAAATAATCT CAGAACAGTG 
GAAGAAATCA TCAGCTTTCA ACACCTTAAG AACTTGTCTT GCTTAAAGTT GTGGCACAAC 
AGCATTTCAT ACGTCCCAGT GCAAATTGGT GCGTTGTCAA ACCTGGAACA ATTGTATCTA 
AATTACAATA ATATTAAAGA TGTTCCATTG CAGCTGTTTC TTTGCAAAAA GTTACAATAT 
TTGGATCTTA GCTATAATAA GCTAACCTCC ATCCCTGAAG AAATCGGTTA TCTGACCAAT 
CTACAGTACT TGGCTTTGAC TAAAAACCAT ATTGAAATGT TGCCTGATAG ATTGTTTCAG 
TGCAAAAAGC TGCAATTTCT TCTTCTGGGA AATAACAGTC TGATGAATTT GTCCCCTCTT 
GTGGGCCAGC TACTCAATCT TGTTCACTTA GAGCTCACTG GAAACTATCT TGAATCACTT 
CCTGCTGAAC TGGAAGAATG TCAGTTCCTA AAACGGAATA GTCTAATTGT AGAAGAAAGG 
TTGTTAAAAA CGCTTCCACC TCGTGTAAGA GATCGCCTGC AGACCAGCTC AGATAAGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8B ( XM_015290645.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146131.1 Gallus gallus leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa11944
Clone ID Related Accession (Same CDS sequence)	XM_004936638.2 , XM_015290645.1
Accession Version	XM_015290645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	volume-regulated anion channel subunit LRRC8B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACGC TAACAGAACT CAAATACTTA GCAGATGCTC AGTCATCCTA CCACATACTA 
AAACCATGGT GGGATGTCTT CTGGTATTAT CTCACCATGA TAATGCTGCT AGTTGCTGTG 
CTTGCTGGGG CTCTCCAGCT TACTCAAACC AGAGTGTTGT GTTGTCTTCC TTGCAAGCTA 
GAATTTGACA ATCATTGTGC CGTGCCTTGG GATCTAATTA AAACCAATCT TAACATGTCT 
GCTAGCTCTA CTGCACAGAT AATCATCCCA CTTAAAATCC AGAATGATCT CCATCGGCAG 
CAGTATTCCT ATATTGATGC AGTATGCTAT GAGAGACAGC TTCATTGGTT TGCCAAATTT 
TTTCCATACC TAGTGCTTCT GCATACCTTT ATTTTTGCAG CTTGCAGTAA TTTCTGGCTT 
TACTATCCTA GTACAAGTTC CAGGCTTGAG CACTTTGTAG CAATTCTTCA GAAGTGTTTT 
GATTCTCCAT GGACAACACG TGCCCTTTCA GAAACAGTTG CTGAGCAGTC TGTGAGGAAA 
TTGCCAGTTG CCAAATCAAA AGCAGTGATT TCATCGCCTG GGAGTTCAGT AGATGTAGGT 
GCCAGCAGAC AGTCCCTGCC ATATACACAA CCTGGCTTGG AAACCACCGG AAGAGAAAAT 
TCAAGTGTTC TTGATAAAAA AGAAGGAGAA CAAGCTAAAG CTATATTTGA AAAAGTGAAG 
AAATTCAGAG TGCATGTTGA AGAGAAGGAT GTCATATATA GAGTGTATAT GAAACAGATC 
ATAGTCAAAG TCATTGTGTT TGTAATAATA ATAATTTATG TTCCCTATTA TTTATCCTTT 
ATTAAACTTG AAATTGATTG CATAGTTAAT GTACAAGCCT TTACAGGCTA CAAAAGGTAC 
CAGTGTGTCT ATTCATTAGC AGAAATTTTT AAAGTTCTGG CTTCATTTTA TGTTGTTTTG 
GTGATCTTCT ATGGCTTAAC TTGTACGTAT AGTTTGTGGT GGATGTTAAG AAGCTCACTT 
AAGCAATATT CTTTTGAGAA GTTGAGAGAG AAAAGTAACT ACAGCGATAT ACCTGATGTA 
AAGAATGACT TTGCATTTAT TCTCCACTTG GCAGATCAGT ATGATCCTCT TTATTCCCAG 
CGATTCTCAA TATTCTTGTC TGATTTGAGT GAAAATAAAC TGAAACAGAT AAATCTGAAC 
AATGAATGGT CAGTGGAAAA ACTGAAAAAT AAGCTAATAA GAAATTCCCA GGACAAGGTT 
GAACTTCACC TTTTCATGTT AAGTGGTCTT CCAGACAGTG TCTTTGAACT GACAGAAATA 
GAAGTCCTAA GCTTGGAACT TATTCCTGAA GCAAAGCTTC CTTCAGCTGT GACCCAGCTA 
GTCAATCTCA AAGATCTCAA CGTTTATCAT TCATCAATAA CTATGGATTA TCCAGCAGTG 
AGCTTTTTAG AAGAAAATCT GAAAACATTG CGTCTGAAAT CTAGTGAGAT GGGAAGAATT 
CCATTTTGGG TATTTCATCT GAAAAACCTC AAAGAATTGT GCTTAACAGG ATATTTCATG 
CTAGATCATC AGAACTCCAT TTACAATGAA AGCTTTCAGG GGCTAAAAAA TCTGAGATCA 
ATTCACTTAA AAAACAACCT TTCTCGCATA CCTCAAGTGG TTACAGATCT GCCTTCTTTG 
CAGCACTTAT CTATCAACAA CGAGGGAAAT AAACTCATAG TGCTAAATAA CTTGAAGAAG 
CTTGTGAACT TGAGAACCTT GGAATTAATC TGCTGTGATC TAGAGCGCAT TCCCCATTCC 
ATTTTTACCC TAAACAACTT GCATGAAATT GACTTAAGAG AAAATAATCT CAGAACAGTG 
GAAGAAATCA TCAGCTTTCA ACACCTTAAG AACTTGTCTT GCTTAAAGTT GTGGCACAAC 
AGCATTTCAT ACGTCCCAGT GCAAATTGGT GCGTTGTCAA ACCTGGAACA ATTGTATCTA 
AATTACAATA ATATTAAAGA TGTTCCATTG CAGCTGTTTC TTTGCAAAAA GTTACAATAT 
TTGGATCTTA GCTATAATAA GCTAACCTCC ATCCCTGAAG AAATCGGTTA TCTGACCAAT 
CTACAGTACT TGGCTTTGAC TAAAAACCAT ATTGAAATGT TGCCTGATAG ATTGTTTCAG 
TGCAAAAAGC TGCAATTTCT TCTTCTGGGA AATAACAGTC TGATGAATTT GTCCCCTCTT 
GTGGGCCAGC TACTCAATCT TGTTCACTTA GAGCTCACTG GAAACTATCT TGAATCACTT 
CCTGCTGAAC TGGAAGAATG TCAGTTCCTA AAACGGAATA GTCTAATTGT AGAAGAAAGG 
TTGTTAAAAA CGCTTCCACC TCGTGTAAGA GATCGCCTGC AGACCAGCTC AGATAAGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146131.1
CDS	257..2659
Translation	MITLTELKYLADAQSSYHILKPWWDVFWYYLTMIMLLVAVLAGALQLTQTRVLCCLPCKLEFDNHCAVPWDLIKTNLNMSASSTAQIIIPLKIQNDLHRQQYSYIDAVCYERQLHWFAKFFPYLVLLHTFIFAACSNFWLYYPSTSSRLEHFVAILQKCFDSPWTTRALSETVAEQSVRKLPVAKSKAVISSPGSSVDVGASRQSLPYTQPGLETTGRENSSVLDKKEGEQAKAIFEKVKKFRVHVEEKDVIYRVYMKQIIVKVIVFVIIIIYVPYYLSFIKLEIDCIVNVQAFTGYKRYQCVYSLAEIFKVLASFYVVLVIFYGLTCTYSLWWMLRSSLKQYSFEKLREKSNYSDIPDVKNDFAFILHLADQYDPLYSQRFSIFLSDLSENKLKQINLNNEWSVEKLKNKLIRNSQDKVELHLFMLSGLPDSVFELTEIEVLSLELIPEAKLPSAVTQLVNLKDLNVYHSSITMDYPAVSFLEENLKTLRLKSSEMGRIPFWVFHLKNLKELCLTGYFMLDHQNSIYNESFQGLKNLRSIHLKNNLSRIPQVVTDLPSLQHLSINNEGNKLIVLNNLKKLVNLRTLELICCDLERIPHSIFTLNNLHEIDLRENNLRTVEEIISFQHLKNLSCLKLWHNSISYVPVQIGALSNLEQLYLNYNNIKDVPLQLFLCKKLQYLDLSYNKLTSIPEEIGYLTNLQYLALTKNHIEMLPDRLFQCKKLQFLLLGNNSLMNLSPLVGQLLNLVHLELTGNYLESLPAELEECQFLKRNSLIVEERLLKTLPPRVRDRLQTSSDKC

Target ORF information:

RefSeq Version	XM_015290645.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015290645.1

ORF Insert Sequence:

ATGATCACGC TAACAGAACT CAAATACTTA GCAGATGCTC AGTCATCCTA CCACATACTA 
AAACCATGGT GGGATGTCTT CTGGTATTAT CTCACCATGA TAATGCTGCT AGTTGCTGTG 
CTTGCTGGGG CTCTCCAGCT TACTCAAACC AGAGTGTTGT GTTGTCTTCC TTGCAAGCTA 
GAATTTGACA ATCATTGTGC CGTGCCTTGG GATCTAATTA AAACCAATCT TAACATGTCT 
GCTAGCTCTA CTGCACAGAT AATCATCCCA CTTAAAATCC AGAATGATCT CCATCGGCAG 
CAGTATTCCT ATATTGATGC AGTATGCTAT GAGAGACAGC TTCATTGGTT TGCCAAATTT 
TTTCCATACC TAGTGCTTCT GCATACCTTT ATTTTTGCAG CTTGCAGTAA TTTCTGGCTT 
TACTATCCTA GTACAAGTTC CAGGCTTGAG CACTTTGTAG CAATTCTTCA GAAGTGTTTT 
GATTCTCCAT GGACAACACG TGCCCTTTCA GAAACAGTTG CTGAGCAGTC TGTGAGGAAA 
TTGCCAGTTG CCAAATCAAA AGCAGTGATT TCATCGCCTG GGAGTTCAGT AGATGTAGGT 
GCCAGCAGAC AGTCCCTGCC ATATACACAA CCTGGCTTGG AAACCACCGG AAGAGAAAAT 
TCAAGTGTTC TTGATAAAAA AGAAGGAGAA CAAGCTAAAG CTATATTTGA AAAAGTGAAG 
AAATTCAGAG TGCATGTTGA AGAGAAGGAT GTCATATATA GAGTGTATAT GAAACAGATC 
ATAGTCAAAG TCATTGTGTT TGTAATAATA ATAATTTATG TTCCCTATTA TTTATCCTTT 
ATTAAACTTG AAATTGATTG CATAGTTAAT GTACAAGCCT TTACAGGCTA CAAAAGGTAC 
CAGTGTGTCT ATTCATTAGC AGAAATTTTT AAAGTTCTGG CTTCATTTTA TGTTGTTTTG 
GTGATCTTCT ATGGCTTAAC TTGTACGTAT AGTTTGTGGT GGATGTTAAG AAGCTCACTT 
AAGCAATATT CTTTTGAGAA GTTGAGAGAG AAAAGTAACT ACAGCGATAT ACCTGATGTA 
AAGAATGACT TTGCATTTAT TCTCCACTTG GCAGATCAGT ATGATCCTCT TTATTCCCAG 
CGATTCTCAA TATTCTTGTC TGATTTGAGT GAAAATAAAC TGAAACAGAT AAATCTGAAC 
AATGAATGGT CAGTGGAAAA ACTGAAAAAT AAGCTAATAA GAAATTCCCA GGACAAGGTT 
GAACTTCACC TTTTCATGTT AAGTGGTCTT CCAGACAGTG TCTTTGAACT GACAGAAATA 
GAAGTCCTAA GCTTGGAACT TATTCCTGAA GCAAAGCTTC CTTCAGCTGT GACCCAGCTA 
GTCAATCTCA AAGATCTCAA CGTTTATCAT TCATCAATAA CTATGGATTA TCCAGCAGTG 
AGCTTTTTAG AAGAAAATCT GAAAACATTG CGTCTGAAAT CTAGTGAGAT GGGAAGAATT 
CCATTTTGGG TATTTCATCT GAAAAACCTC AAAGAATTGT GCTTAACAGG ATATTTCATG 
CTAGATCATC AGAACTCCAT TTACAATGAA AGCTTTCAGG GGCTAAAAAA TCTGAGATCA 
ATTCACTTAA AAAACAACCT TTCTCGCATA CCTCAAGTGG TTACAGATCT GCCTTCTTTG 
CAGCACTTAT CTATCAACAA CGAGGGAAAT AAACTCATAG TGCTAAATAA CTTGAAGAAG 
CTTGTGAACT TGAGAACCTT GGAATTAATC TGCTGTGATC TAGAGCGCAT TCCCCATTCC 
ATTTTTACCC TAAACAACTT GCATGAAATT GACTTAAGAG AAAATAATCT CAGAACAGTG 
GAAGAAATCA TCAGCTTTCA ACACCTTAAG AACTTGTCTT GCTTAAAGTT GTGGCACAAC 
AGCATTTCAT ACGTCCCAGT GCAAATTGGT GCGTTGTCAA ACCTGGAACA ATTGTATCTA 
AATTACAATA ATATTAAAGA TGTTCCATTG CAGCTGTTTC TTTGCAAAAA GTTACAATAT 
TTGGATCTTA GCTATAATAA GCTAACCTCC ATCCCTGAAG AAATCGGTTA TCTGACCAAT 
CTACAGTACT TGGCTTTGAC TAAAAACCAT ATTGAAATGT TGCCTGATAG ATTGTTTCAG 
TGCAAAAAGC TGCAATTTCT TCTTCTGGGA AATAACAGTC TGATGAATTT GTCCCCTCTT 
GTGGGCCAGC TACTCAATCT TGTTCACTTA GAGCTCACTG GAAACTATCT TGAATCACTT 
CCTGCTGAAC TGGAAGAATG TCAGTTCCTA AAACGGAATA GTCTAATTGT AGAAGAAAGG 
TTGTTAAAAA CGCTTCCACC TCGTGTAAGA GATCGCCTGC AGACCAGCTC AGATAAGTGC 
TAA