WRN gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015276195.1	XP_015131681.1	Werner syndrome ATP-dependent helicase isoform X1
XM_015276197.1	XP_015131683.1	Werner syndrome ATP-dependent helicase isoform X3
XM_015276199.1	XP_015131685.1	Werner syndrome ATP-dependent helicase isoform X5
XM_015276197.2	XP_015131683.1	Werner syndrome ATP-dependent helicase isoform X4
XM_015276198.1	XP_015131684.1	Werner syndrome ATP-dependent helicase isoform X4
XM_015276195.2	XP_015131681.1	Werner syndrome ATP-dependent helicase isoform X1
XM_015276196.2	XP_015131682.1	Werner syndrome ATP-dependent helicase isoform X2
XM_025149603.1	XP_025005371.1	Werner syndrome ATP-dependent helicase isoform X5
XM_015276199.2	XP_015131685.1	Werner syndrome ATP-dependent helicase isoform X7
XM_015276201.1	XP_015131687.1	Werner syndrome ATP-dependent helicase isoform X8
NM_001282289.3	NP_001269218.2	Werner syndrome ATP-dependent helicase
XM_015276198.2	XP_015131684.1	Werner syndrome ATP-dependent helicase isoform X6
XM_015276200.1	XP_015131686.1	Werner syndrome ATP-dependent helicase isoform X8
XM_015276196.1	XP_015131682.1	Werner syndrome ATP-dependent helicase isoform X2
XM_025149602.1	XP_025005370.1	Werner syndrome ATP-dependent helicase isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa35062	XM_015276199.2 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa35063	XM_015276201.1 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa35063	XM_015276200.1 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa150085	XM_025149602.1 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa150086	XM_025149603.1 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa35058	XM_015276195.2 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35059	XM_015276196.2 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35060	XM_015276197.2 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35061	XM_015276198.2 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa28582	NM_001282289.3 Latest version!	Gallus gallus Werner syndrome RecQ like helicase (WRN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OGa35060	XM_015276197.1	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35058	XM_015276195.1	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35062	XM_015276199.1	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa35061	XM_015276198.1	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa35059	XM_015276196.1	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

WRN ( XM_015276199.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131685.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa35062
Clone ID Related Accession (Same CDS sequence)	XM_015276199.1 , XM_015276199.2
Accession Version	XM_015276199.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG 
AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT 
GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG 
CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG 
AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT 
TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT 
GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC 
TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG 
TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG 
AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA 
TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG 
AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG 
AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC 
TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG 
AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG 
AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA 
AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT 
GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT 
GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC 
TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA 
GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC 
TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT 
GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG 
GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG 
GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG 
ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA 
GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC 
CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG 
ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA 
GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG 
ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT 
ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA 
ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA 
TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG 
AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA 
CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT 
ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA 
GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA 
ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG 
GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT 
GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG 
CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT 
CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG 
GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT 
AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC 
AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT 
GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT 
AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG 
AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC 
CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA 
ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT 
CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT 
AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA 
CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT 
CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG 
AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC 
AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG 
GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG 
AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG 
ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT 
ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT 
GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA 
TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT 
GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC 
CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT 
GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC 
AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG 
GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT 
GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC 
TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC 
CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC 
CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG 
CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT 
GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131685.1
CDS	408..4901
Translation	MLKVARPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276199.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276199.1

ORF Insert Sequence:

ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG 
AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT 
GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG 
CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG 
AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT 
TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT 
GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC 
TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG 
TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG 
AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA 
TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG 
AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG 
AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC 
TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG 
AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG 
AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA 
AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT 
GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT 
GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC 
TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA 
GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC 
TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT 
GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG 
GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG 
GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG 
ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA 
GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC 
CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG 
ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA 
GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG 
ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT 
ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA 
ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA 
TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG 
AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA 
CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT 
ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA 
GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA 
ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG 
GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT 
GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG 
CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT 
CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG 
GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT 
AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC 
AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT 
GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT 
AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG 
AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC 
CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA 
ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT 
CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT 
AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA 
CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT 
CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG 
AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC 
AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG 
GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG 
AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG 
ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT 
ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT 
GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA 
TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT 
GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC 
CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT 
GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC 
AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG 
GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT 
GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC 
TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC 
CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC 
CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG 
CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT 
GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276199.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131685.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa35062
Clone ID Related Accession (Same CDS sequence)	XM_015276199.1 , XM_015276199.2
Accession Version	XM_015276199.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG 
AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT 
GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG 
CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG 
AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT 
TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT 
GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC 
TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG 
TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG 
AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA 
TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG 
AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG 
AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC 
TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG 
AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG 
AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA 
AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT 
GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT 
GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC 
TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA 
GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC 
TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT 
GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG 
GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG 
GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG 
ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA 
GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC 
CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG 
ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA 
GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG 
ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT 
ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA 
ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA 
TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG 
AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA 
CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT 
ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA 
GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA 
ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG 
GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT 
GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG 
CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT 
CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG 
GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT 
AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC 
AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT 
GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT 
AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG 
AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC 
CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA 
ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT 
CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT 
AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA 
CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT 
CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG 
AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC 
AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG 
GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG 
AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG 
ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT 
ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT 
GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA 
TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT 
GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC 
CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT 
GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC 
AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG 
GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT 
GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC 
TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC 
CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC 
CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG 
CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT 
GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131685.1
CDS	402..4895
Translation	MLKVARPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276199.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276199.2

ORF Insert Sequence:

ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG 
AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT 
GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG 
CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG 
AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT 
TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT 
GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC 
TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG 
TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG 
AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA 
TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG 
AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG 
AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC 
TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG 
AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG 
AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA 
AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT 
GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT 
GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC 
TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA 
GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC 
TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT 
GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG 
GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG 
GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG 
ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA 
GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC 
CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG 
ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA 
GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG 
ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT 
ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA 
ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA 
TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG 
AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA 
CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT 
ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA 
GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA 
ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG 
GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT 
GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG 
CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT 
CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG 
GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT 
AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC 
AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT 
GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT 
AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG 
AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC 
CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA 
ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT 
CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT 
AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA 
CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT 
CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG 
AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC 
AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG 
GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG 
AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG 
ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT 
ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT 
GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA 
TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT 
GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC 
CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT 
GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC 
AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG 
GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT 
GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC 
TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC 
CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC 
CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG 
CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT 
GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276201.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131687.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA.

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CloneID	OGa35063
Clone ID Related Accession (Same CDS sequence)	XM_015276201.1 , XM_015276200.1
Accession Version	XM_015276201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT 
GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA 
GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG 
GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA 
CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT 
GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT 
AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT 
GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA 
GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT 
ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA 
GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT 
CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT 
GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG 
AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT 
TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC 
TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC 
TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT 
TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC 
ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT 
GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC 
AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA 
GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA 
AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG 
CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT 
TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA 
CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG 
GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT 
GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT 
TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT 
GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT 
CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA 
ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA 
GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT 
TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC 
TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA 
ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC 
AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC 
AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA 
ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA 
AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC 
AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG 
TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT 
CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT 
TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT 
GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG 
TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT 
GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT 
GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA 
TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG 
CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC 
AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT 
CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT 
ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT 
CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT 
GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG 
CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG 
GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG 
CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT 
GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG 
AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC 
CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC 
CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131687.1
CDS	1715..5491
Translation	MNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276201.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276201.1

ORF Insert Sequence:

ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT 
GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA 
GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG 
GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA 
CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT 
GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT 
AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT 
GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA 
GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT 
ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA 
GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT 
CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT 
GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG 
AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT 
TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC 
TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC 
TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT 
TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC 
ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT 
GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC 
AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA 
GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA 
AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG 
CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT 
TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA 
CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG 
GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT 
GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT 
TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT 
GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT 
CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA 
ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA 
GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT 
TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC 
TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA 
ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC 
AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC 
AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA 
ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA 
AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC 
AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG 
TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT 
CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT 
TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT 
GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG 
TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT 
GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT 
GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA 
TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG 
CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC 
AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT 
CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT 
ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT 
CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT 
GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG 
CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG 
GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG 
CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT 
GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG 
AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC 
CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC 
CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276200.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131686.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA.

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CloneID	OGa35063
Clone ID Related Accession (Same CDS sequence)	XM_015276201.1 , XM_015276200.1
Accession Version	XM_015276200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT 
GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA 
GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG 
GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA 
CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT 
GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT 
AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT 
GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA 
GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT 
ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA 
GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT 
CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT 
GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG 
AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT 
TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC 
TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC 
TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT 
TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC 
ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT 
GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC 
AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA 
GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA 
AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG 
CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT 
TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA 
CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG 
GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT 
GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT 
TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT 
GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT 
CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA 
ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA 
GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT 
TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC 
TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA 
ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC 
AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC 
AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA 
ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA 
AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC 
AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG 
TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT 
CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT 
TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT 
GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG 
TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT 
GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT 
GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA 
TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG 
CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC 
AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT 
CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT 
ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT 
CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT 
GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG 
CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG 
GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG 
CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT 
GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG 
AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC 
CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC 
CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131686.1
CDS	330..4106
Translation	MNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276200.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276200.1

ORF Insert Sequence:

ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT 
GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA 
GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG 
GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA 
CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT 
GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT 
AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT 
GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA 
GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT 
ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA 
GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT 
CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT 
GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG 
AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT 
TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC 
TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC 
TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT 
TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC 
ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT 
GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC 
AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA 
GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA 
AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG 
CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT 
TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA 
CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG 
GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT 
GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT 
TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT 
GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT 
CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA 
ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA 
GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT 
TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC 
TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA 
ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC 
AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC 
AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA 
ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA 
AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC 
AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG 
TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT 
CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT 
TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT 
GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG 
TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT 
GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT 
GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA 
TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG 
CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC 
AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT 
CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT 
ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT 
CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT 
GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG 
CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG 
GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG 
CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT 
GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG 
AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC 
CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC 
CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_025149602.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005370.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa150085
Clone ID Related Accession (Same CDS sequence)	XM_025149602.1
Accession Version	XM_025149602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGTAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA 
GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA 
GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA 
GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC 
CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005370.1
CDS	69..4616
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_025149602.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149602.1

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGTAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA 
GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA 
GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA 
GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC 
CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_025149603.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005371.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa150086
Clone ID Related Accession (Same CDS sequence)	XM_025149603.1
Accession Version	XM_025149603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT 
GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG 
TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA 
AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA 
GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC 
CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT 
GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT 
GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG 
TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA 
CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC 
AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG 
CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT 
CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC 
CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG 
CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT 
GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT 
CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGGCCACGT GGAGCACAGA TGATGCCCAG CCAGGTAACA GGGGTGCAGC AGACCGCACG 
GAGCCCATCC AGTTGGCCTC CAGGAACCAG CCAGCCCTCA GCCCTGATGA AGAACTGTTT 
GCTGACACTC AGCCAAAGAG CTCCCAGCCC CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT 
GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG CCCCAGACCA AGAAACCCAA AGCAGATACA 
AGAAGAGGGA TCTTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005371.1
CDS	69..4589
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_025149603.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149603.1

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT 
GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG 
TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA 
AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA 
GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC 
CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT 
GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT 
GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG 
TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA 
CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC 
AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG 
CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT 
CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC 
CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG 
CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT 
GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT 
CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGGCCACGT GGAGCACAGA TGATGCCCAG CCAGGTAACA GGGGTGCAGC AGACCGCACG 
GAGCCCATCC AGTTGGCCTC CAGGAACCAG CCAGCCCTCA GCCCTGATGA AGAACTGTTT 
GCTGACACTC AGCCAAAGAG CTCCCAGCCC CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT 
GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG CCCCAGACCA AGAAACCCAA AGCAGATACA 
AGAAGAGGGA TCTTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276195.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131681.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.

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CloneID	OGa35058
Clone ID Related Accession (Same CDS sequence)	XM_015276195.1 , XM_015276195.2
Accession Version	XM_015276195.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG 
CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG 
CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC 
CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG 
CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131681.1
CDS	29..4579
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276195.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276195.1

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG 
CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG 
CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC 
CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG 
CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276195.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131681.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

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CloneID	OGa35058
Clone ID Related Accession (Same CDS sequence)	XM_015276195.1 , XM_015276195.2
Accession Version	XM_015276195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG 
CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG 
CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC 
CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG 
CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131681.1
CDS	69..4619
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276195.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276195.2

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG 
CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG 
CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC 
CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG 
CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276196.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131682.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

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CloneID	OGa35059
Clone ID Related Accession (Same CDS sequence)	XM_015276196.2 , XM_015276196.1
Accession Version	XM_015276196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT 
GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG 
TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA 
AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA 
GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC 
CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT 
GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT 
GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG 
TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA 
CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC 
AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG 
CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT 
CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC 
CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG 
CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT 
GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT 
CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA 
GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA 
GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA 
GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC 
CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131682.1
CDS	69..4616
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276196.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276196.2

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT 
GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG 
TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA 
AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA 
GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC 
CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT 
GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT 
GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG 
TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA 
CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC 
AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG 
CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT 
CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC 
CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG 
CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT 
GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT 
CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA 
GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA 
GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA 
GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC 
CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276196.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131682.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa35059
Clone ID Related Accession (Same CDS sequence)	XM_015276196.2 , XM_015276196.1
Accession Version	XM_015276196.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT 
GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG 
TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA 
AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA 
GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC 
CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT 
GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT 
GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG 
TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA 
CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC 
AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG 
CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT 
CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC 
CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG 
CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT 
GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT 
CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA 
GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA 
GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA 
GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC 
CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131682.1
CDS	29..4576
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276196.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276196.1

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT 
GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG 
TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA 
AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA 
GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC 
CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT 
GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT 
GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG 
TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA 
CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC 
AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG 
CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT 
CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC 
CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG 
CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT 
GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT 
CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT 
TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT 
AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA 
AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT 
CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG 
CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC 
AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA 
ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG 
GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT 
CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC 
TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT 
CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC 
CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT 
CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT 
ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA 
TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG 
ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG 
ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT 
TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA 
GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA 
GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA 
GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC 
CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276197.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131683.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.

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CloneID	OGa35060
Clone ID Related Accession (Same CDS sequence)	XM_015276197.1 , XM_015276197.2
Accession Version	XM_015276197.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC 
ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG 
TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG 
TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT 
ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131683.1
CDS	29..4552
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276197.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276197.1

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC 
ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG 
TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG 
TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT 
ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276197.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131683.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

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CloneID	OGa35060
Clone ID Related Accession (Same CDS sequence)	XM_015276197.1 , XM_015276197.2
Accession Version	XM_015276197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC 
ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG 
TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG 
TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT 
ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131683.1
CDS	69..4592
Translation	MLGRGGCFADAVSVRRASRMGAEPEPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276197.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276197.2

ORF Insert Sequence:

ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG 
GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG 
ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC 
TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG 
ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA 
GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG 
TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA 
ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT 
GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT 
AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA 
GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG 
TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT 
AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG 
GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT 
TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT 
GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG 
AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA 
GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT 
ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT 
GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC 
ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA 
GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC 
TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG 
GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC 
ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG 
GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA 
TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA 
GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG 
TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG 
GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT 
CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA 
CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT 
GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT 
TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA 
ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT 
CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG 
AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG 
TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT 
ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT 
CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT 
ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT 
GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT 
CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA 
AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG 
AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA 
CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG 
TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA 
AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA 
GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA 
CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG 
CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG 
TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA 
AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG 
TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA 
GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA 
AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG 
CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG 
TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA 
ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG 
AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT 
AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA 
GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC 
ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT 
CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC 
AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT 
AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA 
CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT 
AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA 
AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC 
TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA 
GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC 
ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG 
TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG 
TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT 
ACAAGAAGAG GGATCTTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276198.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131684.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.

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CloneID	OGa35061
Clone ID Related Accession (Same CDS sequence)	XM_015276198.1 , XM_015276198.2
Accession Version	XM_015276198.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC 
TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG 
ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG 
CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT 
CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA 
TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA 
ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC 
AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC 
TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT 
GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC 
TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA 
CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT 
CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA 
GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG 
GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA 
CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT 
TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA 
GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT 
TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT 
GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC 
ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG 
GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA 
GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG 
CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC 
AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA 
GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC 
CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA 
GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT 
GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC 
TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA 
TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA 
TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT 
ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG 
TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA 
TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG 
AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG 
TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT 
AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA 
AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA 
TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT 
GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC 
CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA 
AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG 
GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG 
AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC 
CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA 
GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT 
GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG 
GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT 
CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC 
TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG 
CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA 
GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG 
AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG 
AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG 
AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA 
CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA 
GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA 
ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT 
ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG 
GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA 
TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT 
CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG 
ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG 
GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG 
ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA 
ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG 
TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG 
CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG 
TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC 
CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG 
TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC 
CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT 
CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA 
GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG 
ATCTTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131684.1
CDS	142..4713
Translation	MLLLMQPKILLAFWAGSTRCWLVLNLSSTSTPRFFSSGLLSSHSPPSLYLCLGFPQPRFRTLHLDLLTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276198.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276198.1

ORF Insert Sequence:

ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC 
TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG 
ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG 
CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT 
CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA 
TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA 
ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC 
AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC 
TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT 
GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC 
TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA 
CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT 
CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA 
GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG 
GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA 
CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT 
TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA 
GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT 
TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT 
GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC 
ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG 
GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA 
GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG 
CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC 
AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA 
GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC 
CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA 
GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT 
GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC 
TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA 
TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA 
TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT 
ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG 
TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA 
TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG 
AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG 
TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT 
AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA 
AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA 
TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT 
GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC 
CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA 
AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG 
GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG 
AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC 
CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA 
GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT 
GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG 
GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT 
CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC 
TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG 
CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA 
GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG 
AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG 
AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG 
AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA 
CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA 
GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA 
ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT 
ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG 
GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA 
TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT 
CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG 
ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG 
GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG 
ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA 
ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG 
TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG 
CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG 
TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC 
CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG 
TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC 
CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT 
CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA 
GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG 
ATCTTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_015276198.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131684.1 Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

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CloneID	OGa35061
Clone ID Related Accession (Same CDS sequence)	XM_015276198.1 , XM_015276198.2
Accession Version	XM_015276198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC 
TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG 
ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG 
CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT 
CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA 
TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA 
ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC 
AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC 
TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT 
GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC 
TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA 
CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT 
CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA 
GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG 
GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA 
CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT 
TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA 
GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT 
TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT 
GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC 
ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG 
GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA 
GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG 
CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC 
AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA 
GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC 
CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA 
GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT 
GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC 
TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA 
TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA 
TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT 
ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG 
TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA 
TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG 
AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG 
TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT 
AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA 
AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA 
TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT 
GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC 
CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA 
AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG 
GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG 
AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC 
CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA 
GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT 
GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG 
GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT 
CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC 
TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG 
CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA 
GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG 
AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG 
AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG 
AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA 
CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA 
GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA 
ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT 
ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG 
GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA 
TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT 
CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG 
ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG 
GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG 
ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA 
ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG 
TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG 
CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG 
TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC 
CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG 
TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC 
CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT 
CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA 
GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG 
ATCTTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131684.1
CDS	129..4700
Translation	MLLLMQPKILLAFWAGSTRCWLVLNLSSTSTPRFFSSGLLSSHSPPSLYLCLGFPQPRFRTLHLDLLTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALPPLLLSYGKATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	XM_015276198.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276198.2

ORF Insert Sequence:

ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC 
TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG 
ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG 
CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT 
CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA 
TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA 
ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC 
AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC 
TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT 
GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC 
TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA 
CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT 
CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA 
GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG 
GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA 
CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT 
TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA 
GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT 
TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT 
GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC 
ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG 
GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA 
GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG 
CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC 
AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA 
GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC 
CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA 
GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT 
GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC 
TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA 
TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA 
TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT 
ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG 
TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA 
TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG 
AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG 
TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT 
AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA 
AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA 
TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT 
GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC 
CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA 
AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG 
GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG 
AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC 
CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA 
GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT 
GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG 
GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT 
CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC 
TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG 
CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA 
GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG 
AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG 
AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG 
AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA 
CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA 
GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA 
ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT 
ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG 
GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA 
TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT 
CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG 
ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG 
GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG 
ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA 
ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG 
TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG 
CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG 
TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC 
CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG 
TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC 
CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT 
CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA 
GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG 
ATCTTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( NM_001282289.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001269218.2 Gallus gallus Werner syndrome RecQ like helicase (WRN), mRNA.

Price & Availability↑

CloneID	OGa28582
Clone ID Related Accession (Same CDS sequence)	NM_001282289.3
Accession Version	NM_001282289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Gallus gallus(chicken)
Product	Werner syndrome ATP-dependent helicase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000017.1. On Feb 11, 2016 this sequence version replaced NM_001282289.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB035866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201366, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END##

ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG 
AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT 
GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG 
CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG 
AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT 
TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT 
GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC 
TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG 
TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG 
AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA 
TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG 
AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG 
AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC 
TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG 
AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG 
AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA 
AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT 
GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT 
GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC 
TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA 
GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC 
TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT 
GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG 
GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG 
GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG 
ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA 
GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC 
CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG 
ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA 
GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG 
ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT 
ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA 
ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA 
TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG 
AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA 
CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT 
ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA 
GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA 
ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG 
GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT 
GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG 
CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT 
CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG 
GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT 
AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC 
AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT 
GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT 
AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG 
AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC 
CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA 
ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT 
CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT 
AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA 
CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT 
CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG 
AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC 
AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG 
GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG 
AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG 
ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT 
ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT 
GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA 
TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT 
GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC 
CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT 
GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC 
AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG 
GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT 
GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC 
TCAGCTTTGG CCACGTGGAG CACAGATGAT GCCCAGCCAG GTAACAGGGG TGCAGCAGAC 
CGCACGGAGC CCATCCAGTT GGCCTCCAGG AACCAGCCAG CCCTCAGCCC TGATGAAGAA 
CTGTTTGCTG ACACTCAGCC AAAGAGCTCC CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG 
TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC CCTGTGCCCC AGACCAAGAA ACCCAAAGCA 
GATACAAGAA GAGGGATCTT CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269218.2
CDS	99..4565
Translation	MLKVARPGNTNVDKMSKVDLQDKYPQWMCARNQEGILSDKKTPNVQRSVLEDGLPFLAFRGSIVYSYEASDCSLLSEDIRQTLSDGAAVGFDIEWPPSYTKGKMAKIALIQLCVTEEKCYLFHISSMSGFPKGLKRLLEDETIKKVGVGIEGDHWKLMGDFEVKLKSFVELADVANEKLKCKEVWSLNGLVKHLFGKQLLKDKSIRCGNWEKFPLDEEQKLYAATDAYAGFIIYQKLKTMNSIDRKLFGERRDEVLSDDVKERLTSLAEGMSYLAAKISDSSGQLRNLQRAAEILADISENVKTLGSTLFAGSSSPSVLERSCGATPTKLEAKTVNVEAQKAEMPEFAKKLEGDFNICSDATLAGLWEGEIDEGEAEGGNAVVEDGATAAGSKDTADRDDFMSLDFNEQELQILERQAAEEVLNKAVPEEECPTDNDQNTSCIIESDEELEMEMLKSLEDVDNSKEVPTERESSKARKTPDTELNVAPDGDEDEVIEEDEEECWDPLLSVPSEIHIACLKTYFGHSSFKPVQWKVIHSVLEDKRDNLVVMATGYGKSLCYQFPPVYTGGTGIVICPLISLMEDQVLQLTMSGISSCLLGSAQSKDVKDNIKAGLYRVIYMTPEFCSGNLELLQDIDRTTGIALIAVDEAHCISEWGHDFRISFRKLNSLKKALPSVPIVALTATASPSIREDIVNCLNLKNPQVTCTSFDRPNLYLEVGRQSGNTLRDLKQFLTRKGSSSTYEFEGPTIIYCPSRKATEQVMFELNKLGVTCGAYHAGMGIQKRRDTHHQFMRDEIQCVVATVAFGMGINKADIRMVIHYGAPKEMESYYQEIGRAGRDGLPASCHVLWTAADLVLNRRLLNEIHNEKFRLYKLKMLEKMEKYLSSNSCRRKIILSHFEDKQLRKVSSGIMGTEECCDNCRCRTSCLATSSDSDGGLQDFGKQAYQLLSAVSALEEKFGTRVPILFLRGSNSQRLPDRYRKHPLYGSGKDWTENWWKSLSQQLIMEGFLREVSGRSKFATTCGLTQKGRNWLLKAGSASNPSLLLQSNEDLNLQRTPRSNRSSPAVSVQRSPDMKGSKQSPVKQMSLYEMFAHEKKAGTLPRSNTMFNSPPERSPVKSPLRPRVPVVSSRENELETALYGKLLTARQKVANEKVIPPAVLATNKILVEMARMRPTTVENVKRIDGVSEAKATMLVLLLAEIKEFCQANGLQTDIFPKPESTDQKETSPWKNTKALSPSEHVTYVLFQEKNLSLRTISETRSLPLTEVGTHLFRAVKAGYPVNLERAGLTPEVQQIISDIIRNPPIDSDTTKIQAIRKLVPAHIELYLIQMAIVLLEKRDGSKSKYGSGAKRMLVLSDKQQEKTGADQTSRGKALWSGRKDDLTNPILKEGDEKNHPQVVPSALATWSTDDAQPGNRGAADRTEPIQLASRNQPALSPDEELFADTQPKSSQPPGKRKVPAWFGASAAPVLPVPQTKKPKADTRRGIFN

Target ORF information:

RefSeq Version	NM_001282289.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Werner syndrome RecQ like helicase (WRN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282289.3

ORF Insert Sequence:

ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG 
AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT 
GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG 
CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG 
AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT 
TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT 
GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC 
TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG 
TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG 
AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA 
TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG 
AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG 
AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC 
TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG 
AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG 
AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA 
AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT 
GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT 
GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC 
TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA 
GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC 
TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT 
GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG 
GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG 
GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG 
ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA 
GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC 
CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG 
ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA 
GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG 
ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT 
ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA 
ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA 
TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG 
AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA 
CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT 
ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA 
GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA 
ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG 
GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT 
GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG 
CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT 
CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG 
GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT 
AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC 
AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT 
GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT 
AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG 
AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC 
CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA 
ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT 
CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT 
AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA 
CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT 
CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG 
AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC 
AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG 
GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG 
AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG 
ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT 
ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT 
GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA 
TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT 
GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC 
CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT 
GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC 
AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG 
GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT 
GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC 
TCAGCTTTGG CCACGTGGAG CACAGATGAT GCCCAGCCAG GTAACAGGGG TGCAGCAGAC 
CGCACGGAGC CCATCCAGTT GGCCTCCAGG AACCAGCCAG CCCTCAGCCC TGATGAAGAA 
CTGTTTGCTG ACACTCAGCC AAAGAGCTCC CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG 
TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC CCTGTGCCCC AGACCAAGAA ACCCAAAGCA 
GATACAAGAA GAGGGATCTT CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WRN
Entrez Gene ID	422505
Full Name	Werner syndrome RecQ like helicase
General protein information	Preferred Names Werner syndrome RecQ like helicase Names Werner syndrome ATP-dependent helicase Werner syndrome protein Werner syndrome, RecQ helicase-like
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	WRN	NP_000544.2
Mus musculus (house mouse)	Wrn	NP_035851.3
Bos taurus (cattle)	WRN	NP_001137329.2
Caenorhabditis elegans (roundworm)	wrn-1	NP_495324.2
Canis lupus familiaris (dog)	WRN	XP_539984.4
Gallus gallus (chicken)	WRN	XP_001235033.2
Xenopus tropicalis (tropical clawed frog)	wrn	XP_002939304.1
Pan troglodytes (chimpanzee)	WRN	XP_528104.2
	WRN	XP_001085031.1
Rattus norvegicus (Norway rat)	RGD1564788	XP_006253317.1
Danio rerio (zebrafish)	wrn	XP_697980.5
Oryza sativa (rice)	Os07g0681600	NP_001060655.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WRN cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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