WRN cDNA ORF clone, Gallus gallus(chicken)

The following WRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa35062 XM_015276199.2
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa35063 XM_015276201.1
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa35063 XM_015276200.1
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa150085 XM_025149602.1
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa150086 XM_025149603.1
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa35058 XM_015276195.2
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35059 XM_015276196.2
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35060 XM_015276197.2
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35061 XM_015276198.2
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa28582 NM_001282289.3
Latest version!
Gallus gallus Werner syndrome RecQ like helicase (WRN), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OGa35060 XM_015276197.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35058 XM_015276195.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35062 XM_015276199.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa35061 XM_015276198.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa35059 XM_015276196.1 Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa35062
    Clone ID Related Accession (Same CDS sequence) XM_015276199.1 , XM_015276199.2
    Accession Version XM_015276199.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
    CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG
    AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT
    GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG
    CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG
    AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT
    TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT
    GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC
    TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG
    TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG
    AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA
    TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG
    AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG
    AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC
    TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG
    AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG
    AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA
    AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT
    GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT
    GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC
    TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA
    GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC
    TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT
    GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG
    GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG
    GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG
    ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA
    GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC
    CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG
    ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA
    GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG
    ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT
    ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA
    ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA
    TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG
    AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA
    CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT
    ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA
    GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA
    ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG
    GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT
    GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG
    CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT
    CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG
    GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT
    AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC
    AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT
    GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT
    AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG
    AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC
    CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA
    ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT
    CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT
    AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA
    CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT
    CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG
    AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC
    AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG
    GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG
    AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG
    ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT
    ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT
    GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA
    TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT
    GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC
    CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT
    GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC
    AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG
    GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT
    GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC
    TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC
    CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC
    CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG
    CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT
    GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131685.1
    CDS408..4901
    Translation

    Target ORF information:

    RefSeq Version XM_015276199.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276199.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
    CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG
    AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT
    GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG
    CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG
    AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT
    TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT
    GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC
    TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG
    TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG
    AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA
    TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG
    AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG
    AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC
    TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG
    AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG
    AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA
    AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT
    GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT
    GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC
    TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA
    GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC
    TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT
    GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG
    GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG
    GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG
    ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA
    GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC
    CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG
    ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA
    GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG
    ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT
    ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA
    ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA
    TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG
    AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA
    CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT
    ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA
    GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA
    ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG
    GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT
    GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG
    CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT
    CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG
    GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT
    AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC
    AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT
    GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT
    AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG
    AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC
    CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA
    ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT
    CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT
    AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA
    CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT
    CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG
    AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC
    AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG
    GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG
    AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG
    ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT
    ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT
    GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA
    TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT
    GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC
    CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT
    GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC
    AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG
    GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT
    GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC
    TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC
    CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC
    CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG
    CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT
    GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35062
    Clone ID Related Accession (Same CDS sequence) XM_015276199.1 , XM_015276199.2
    Accession Version XM_015276199.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
    CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG
    AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT
    GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG
    CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG
    AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT
    TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT
    GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC
    TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG
    TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG
    AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA
    TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG
    AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG
    AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC
    TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG
    AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG
    AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA
    AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT
    GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT
    GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC
    TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA
    GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC
    TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT
    GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG
    GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG
    GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG
    ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA
    GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC
    CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG
    ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA
    GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG
    ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT
    ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA
    ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA
    TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG
    AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA
    CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT
    ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA
    GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA
    ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG
    GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT
    GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG
    CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT
    CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG
    GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT
    AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC
    AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT
    GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT
    AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG
    AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC
    CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA
    ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT
    CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT
    AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA
    CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT
    CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG
    AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC
    AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG
    GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG
    AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG
    ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT
    ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT
    GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA
    TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT
    GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC
    CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT
    GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC
    AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG
    GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT
    GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC
    TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC
    CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC
    CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG
    CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT
    GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131685.1
    CDS402..4895
    Translation

    Target ORF information:

    RefSeq Version XM_015276199.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
    CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG
    AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT
    GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG
    CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG
    AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT
    TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT
    GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC
    TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG
    TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG
    AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA
    TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG
    AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG
    AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC
    TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG
    AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG
    AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA
    AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT
    GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT
    GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC
    TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA
    GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC
    TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT
    GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG
    GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG
    GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG
    ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA
    GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC
    CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG
    ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA
    GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG
    ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT
    ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA
    ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA
    TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG
    AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA
    CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT
    ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA
    GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA
    ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG
    GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT
    GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG
    CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT
    CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG
    GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT
    AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC
    AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT
    GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT
    AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG
    AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC
    CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA
    ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT
    CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT
    AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA
    CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT
    CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG
    AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC
    AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG
    GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG
    AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG
    ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT
    ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT
    GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA
    TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT
    GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC
    CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT
    GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC
    AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG
    GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT
    GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC
    TCAGCTTTGC CTCCTTTGTT ACTGTCCTAT GGAAAGGCCA CGTGGAGCAC AGATGATGCC
    CAGCCAGGTA ACAGGGGTGC AGCAGACCGC ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC
    CAGCCAGCCC TCAGCCCTGA TGAAGAACTG TTTGCTGACA CTCAGCCAAA GAGCTCCCAG
    CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT
    GTGCCCCAGA CCAAGAAACC CAAAGCAGAT ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35063
    Clone ID Related Accession (Same CDS sequence) XM_015276201.1 , XM_015276200.1
    Accession Version XM_015276201.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
    GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT
    GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA
    GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG
    GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA
    CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT
    GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT
    AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT
    GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA
    GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT
    ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA
    GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT
    CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT
    GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG
    AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT
    TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC
    TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC
    TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT
    TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC
    ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT
    GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC
    AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA
    GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA
    AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG
    CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT
    TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA
    CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG
    GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT
    GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT
    TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT
    GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT
    CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA
    ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA
    GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT
    TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC
    TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA
    ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC
    AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC
    AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA
    ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA
    AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC
    AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG
    TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT
    CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT
    TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT
    GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG
    TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT
    GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT
    GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA
    TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG
    CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC
    AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT
    CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT
    ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT
    CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT
    GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG
    CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG
    GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG
    CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT
    GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG
    AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC
    CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC
    CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131687.1
    CDS1715..5491
    Translation

    Target ORF information:

    RefSeq Version XM_015276201.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276201.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
    GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT
    GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA
    GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG
    GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA
    CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT
    GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT
    AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT
    GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA
    GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT
    ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA
    GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT
    CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT
    GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG
    AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT
    TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC
    TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC
    TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT
    TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC
    ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT
    GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC
    AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA
    GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA
    AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG
    CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT
    TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA
    CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG
    GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT
    GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT
    TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT
    GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT
    CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA
    ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA
    GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT
    TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC
    TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA
    ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC
    AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC
    AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA
    ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA
    AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC
    AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG
    TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT
    CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT
    TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT
    GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG
    TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT
    GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT
    GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA
    TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG
    CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC
    AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT
    CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT
    ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT
    CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT
    GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG
    CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG
    GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG
    CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT
    GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG
    AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC
    CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC
    CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35063
    Clone ID Related Accession (Same CDS sequence) XM_015276201.1 , XM_015276200.1
    Accession Version XM_015276200.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
    GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT
    GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA
    GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG
    GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA
    CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT
    GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT
    AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT
    GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA
    GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT
    ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA
    GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT
    CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT
    GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG
    AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT
    TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC
    TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC
    TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT
    TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC
    ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT
    GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC
    AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA
    GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA
    AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG
    CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT
    TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA
    CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG
    GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT
    GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT
    TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT
    GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT
    CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA
    ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA
    GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT
    TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC
    TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA
    ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC
    AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC
    AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA
    ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA
    AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC
    AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG
    TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT
    CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT
    TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT
    GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG
    TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT
    GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT
    GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA
    TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG
    CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC
    AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT
    CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT
    ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT
    CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT
    GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG
    CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG
    GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG
    CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT
    GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG
    AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC
    CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC
    CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131686.1
    CDS330..4106
    Translation

    Target ORF information:

    RefSeq Version XM_015276200.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAGCA TTGACAGGAA ATTATTTGGG GAAAGAAGAG ATGAAGTGTT GTCAGACGAC 
    GTGAAGGAGC GCTTAACTTC GCTAGCTGAA GGGATGTCAT ACCTGGCTGC CAAGATCTCT
    GACTCTTCTG GACAACTCAG AAATTTGCAG AGGGCTGCAG AAATACTAGC TGACATATCA
    GAGAATGTAA AAACCTTAGG AAGTACACTC TTTGCTGGTT CAAGTTCTCC TTCTGTACTG
    GAGAGGAGCT GTGGTGCAAC TCCAACAAAA CTTGAAGCAA AGACAGTGAA TGTTGAAGCA
    CAAAAAGCTG AAATGCCTGA ATTTGCTAAA AAACTTGAAG GTGATTTCAA CATCTGCTCT
    GATGCTACAC TAGCTGGCCT CTGGGAGGGA GAAATTGATG AAGGTGAAGC AGAGGGAGGT
    AATGCTGTTG TTGAAGATGG GGCTACAGCA GCTGGCTCTA AGGACACAGC AGACAGAGAT
    GACTTCATGT CGTTAGACTT TAATGAGCAA GAACTACAGA TATTGGAACG TCAGGCTGCA
    GAAGAAGTTC TCAACAAAGC TGTGCCTGAG GAGGAATGCC CAACAGACAA CGACCAAAAT
    ACCTCCTGTA TCATTGAAAG TGATGAAGAA CTGGAAATGG AGATGCTTAA ATCTTTAGAA
    GATGTGGACA ATTCTAAAGA AGTTCCAACT GAAAGGGAGT CCAGCAAAGC CAGGAAAACT
    CCGGACACAG AATTGAACGT CGCACCAGAT GGGGATGAAG ATGAAGTCAT TGAAGAGGAT
    GAGGAGGAAT GTTGGGATCC ATTGCTGTCA GTGCCTAGTG AAATCCACAT CGCATGCCTG
    AAGACATATT TTGGGCATTC TTCTTTTAAA CCGGTTCAGT GGAAAGTGAT ACATTCTGTT
    TTAGAAGACA AAAGAGACAA TCTTGTTGTC ATGGCAACTG GGTATGGGAA GAGTTTGTGC
    TACCAGTTTC CTCCTGTTTA CACTGGAGGT ACAGGAATAG TCATCTGTCC TCTTATTTCC
    TTGATGGAGG ACCAAGTTCT GCAACTGACA ATGTCAGGGA TTTCATCTTG CTTGCTTGGT
    TCAGCTCAGT CAAAAGATGT CAAAGACAAC ATTAAAGCGG GTCTATACAG AGTCATATAC
    ATGACACCAG AGTTCTGTTC AGGGAATTTA GAGCTACTTC AGGATATTGA CCGAACTACT
    GGTATTGCTC TCATAGCTGT TGATGAAGCT CACTGCATTT CTGAGTGGGG CCATGACTTC
    AGAATTTCCT TTAGAAAGTT AAACTCATTG AAGAAGGCTC TACCATCGGT TCCCATTGTA
    GCATTAACAG CAACTGCAAG TCCCTCAATT AGAGAAGACA TAGTGAATTG TCTGAACCTA
    AAGAATCCTC AGGTCACATG TACTAGCTTT GACAGACCTA ATCTGTACTT AGAAGTTGGG
    CGACAGAGTG GAAATACCCT TAGGGATTTG AAGCAGTTCC TTACTAGGAA AGGAAGCAGT
    TCTACGTATG AGTTTGAAGG CCCCACTATC ATCTACTGCC CTTCAAGAAA GGCCACTGAA
    CAAGTTATGT TTGAACTGAA TAAACTTGGT GTGACTTGTG GTGCATACCA TGCTGGCATG
    GGAATTCAAA AAAGGAGAGA TACCCATCAC CAGTTCATGC GGGATGAAAT CCAGTGTGTT
    GTGGCTACAG TGGCATTTGG AATGGGCATC AATAAAGCAG ATATCCGGAT GGTTATTCAT
    TATGGTGCCC CCAAGGAAAT GGAATCCTAT TACCAAGAAA TTGGAAGAGC TGGTCGTGAT
    GGGCTTCCTG CTTCTTGTCA CGTCTTGTGG ACTGCAGCAG ACTTGGTTTT AAACAGGCGT
    CTTCTAAATG AGATACACAA TGAAAAGTTC AGATTGTACA AACTGAAGAT GTTAGAAAAA
    ATGGAGAAGT ACCTCTCCTC AAACAGCTGC AGAAGAAAAA TCATCCTGTC TCATTTTGAA
    GATAAACAAC TCCGAAAGGT CTCCTCTGGC ATTATGGGCA CAGAAGAGTG CTGTGATAAT
    TGCAGGTGCA GAACCTCTTG TTTGGCAACC TCCAGTGATT CAGATGGTGG TTTACAGGAC
    TTTGGAAAGC AAGCATATCA GCTGCTTTCT GCAGTAAGCG CCCTGGAAGA GAAGTTTGGA
    ACTAGAGTTC CCATTTTGTT TCTCCGAGGT TCTAATTCTC AGCGTCTGCC AGACAGATAC
    AGGAAACACC CTCTGTACGG TAGTGGAAAA GACTGGACAG AGAACTGGTG GAAATCACTC
    AGCCAGCAGC TGATAATGGA AGGATTCCTC AGAGAAGTCT CTGGGCGTAG CAAGTTTGCA
    ACAACGTGTG GGCTTACCCA GAAGGGTAGA AATTGGCTTT TGAAAGCCGG ATCTGCCTCA
    AATCCAAGTC TTCTGTTACA GTCCAATGAA GACTTAAACC TACAGAGAAC TCCTAGAAGC
    AATAGGTCTT CACCTGCAGT CTCAGTGCAG CGCTCCCCAG ACATGAAAGG ATCAAAGCAG
    TCACCAGTTA AGCAGATGTC TTTGTATGAA ATGTTTGCAC ATGAGAAAAA AGCTGGAACT
    CTTCCAAGAA GCAATACCAT GTTTAACAGC CCCCCAGAGC GCTCACCAGT GAAATCTCCT
    TTGAGGCCTC GAGTACCTGT TGTTTCATCC CGAGAAAATG AACTAGAGAC TGCTCTCTAT
    GGCAAGTTGC TGACTGCTAG ACAGAAGGTA GCTAATGAGA AGGTAATTCC TCCTGCTGTG
    TTGGCAACCA ATAAGATCCT GGTGGAGATG GCCCGAATGA GGCCTACCAC TGTTGAGAAT
    GTGAAGAGAA TTGATGGTGT ATCTGAAGCC AAAGCGACGA TGTTGGTCCT TCTTCTAGCT
    GAGATTAAGG AGTTCTGCCA AGCAAATGGT TTGCAGACGG ACATATTCCC CAAACCTGAA
    TCTACAGATC AGAAAGAGAC ATCTCCTTGG AAGAATACTA AAGCTCTGTC TCCCTCAGAG
    CATGTCACCT ATGTCCTGTT CCAAGAGAAA AATCTCTCCC TGAGGACTAT CAGTGAGACC
    AGATCTCTTC CTCTTACTGA GGTTGGCACT CACTTGTTCC GGGCGGTGAA AGCTGGCTAT
    CCTGTCAACC TGGAGCGAGC AGGTTTGACT CCTGAAGTTC AACAGATCAT TTCTGATATT
    ATCCGTAATC CTCCTATTGA CTCAGACACC ACCAAAATTC AAGCAATTAG AAAACTTGTT
    CCTGCACATA TTGAACTGTA TCTCATCCAG ATGGCTATTG TGTTGTTGGA GAAAAGGGAT
    GGCAGTAAAT CTAAGTATGG TTCAGGTGCC AAAAGGATGC TGGTGCTGTC AGACAAGCAG
    CAGGAAAAGA CAGGAGCAGA CCAAACAAGT AGGGGCAAAG CTTTGTGGAG TGGAAGAAAG
    GATGACTTGA CCAATCCTAT ACTGAAGGAA GGAGATGAAA AGAACCATCC ACAAGTGGTG
    CCCTCAGCTT TGCCTCCTTT GTTACTGTCC TATGGAAAGG CCACGTGGAG CACAGATGAT
    GCCCAGCCAG GTAACAGGGG TGCAGCAGAC CGCACGGAGC CCATCCAGTT GGCCTCCAGG
    AACCAGCCAG CCCTCAGCCC TGATGAAGAA CTGTTTGCTG ACACTCAGCC AAAGAGCTCC
    CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC
    CCTGTGCCCC AGACCAAGAA ACCCAAAGCA GATACAAGAA GAGGGATCTT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150085
    Clone ID Related Accession (Same CDS sequence) XM_025149602.1
    Accession Version XM_025149602.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGTAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA
    GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA
    GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA
    GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC
    CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005370.1
    CDS69..4616
    Translation

    Target ORF information:

    RefSeq Version XM_025149602.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGTAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA
    GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA
    GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA
    GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC
    CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150086
    Clone ID Related Accession (Same CDS sequence) XM_025149603.1
    Accession Version XM_025149603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT
    GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG
    TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA
    AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA
    GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC
    CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT
    GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT
    GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG
    TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA
    CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC
    AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG
    CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT
    CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC
    CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG
    CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT
    GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT
    CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGGCCACGT GGAGCACAGA TGATGCCCAG CCAGGTAACA GGGGTGCAGC AGACCGCACG
    GAGCCCATCC AGTTGGCCTC CAGGAACCAG CCAGCCCTCA GCCCTGATGA AGAACTGTTT
    GCTGACACTC AGCCAAAGAG CTCCCAGCCC CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT
    GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG CCCCAGACCA AGAAACCCAA AGCAGATACA
    AGAAGAGGGA TCTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005371.1
    CDS69..4589
    Translation

    Target ORF information:

    RefSeq Version XM_025149603.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT
    GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG
    TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA
    AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA
    GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC
    CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT
    GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT
    GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG
    TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA
    CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC
    AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG
    CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT
    CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC
    CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG
    CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT
    GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT
    CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGGCCACGT GGAGCACAGA TGATGCCCAG CCAGGTAACA GGGGTGCAGC AGACCGCACG
    GAGCCCATCC AGTTGGCCTC CAGGAACCAG CCAGCCCTCA GCCCTGATGA AGAACTGTTT
    GCTGACACTC AGCCAAAGAG CTCCCAGCCC CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT
    GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG CCCCAGACCA AGAAACCCAA AGCAGATACA
    AGAAGAGGGA TCTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35058
    Clone ID Related Accession (Same CDS sequence) XM_015276195.1 , XM_015276195.2
    Accession Version XM_015276195.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG
    CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG
    CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC
    CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG
    CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131681.1
    CDS29..4579
    Translation

    Target ORF information:

    RefSeq Version XM_015276195.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG
    CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG
    CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC
    CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG
    CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35058
    Clone ID Related Accession (Same CDS sequence) XM_015276195.1 , XM_015276195.2
    Accession Version XM_015276195.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG
    CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG
    CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC
    CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG
    CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131681.1
    CDS69..4619
    Translation

    Target ORF information:

    RefSeq Version XM_015276195.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGCCTC CTTTGTTACT GTCCTATGGA AAGGCCACGT GGAGCACAGA TGATGCCCAG
    CCAGGTAACA GGGGTGCAGC AGACCGCACG GAGCCCATCC AGTTGGCCTC CAGGAACCAG
    CCAGCCCTCA GCCCTGATGA AGAACTGTTT GCTGACACTC AGCCAAAGAG CTCCCAGCCC
    CCAGGTAAGA GGAAGGTGCC TGCGTGGTTT GGAGCCTCAG CAGCCCCTGT TCTCCCTGTG
    CCCCAGACCA AGAAACCCAA AGCAGATACA AGAAGAGGGA TCTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35059
    Clone ID Related Accession (Same CDS sequence) XM_015276196.2 , XM_015276196.1
    Accession Version XM_015276196.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT
    GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG
    TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA
    AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA
    GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC
    CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT
    GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT
    GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG
    TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA
    CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC
    AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG
    CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT
    CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC
    CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG
    CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT
    GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT
    CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA
    GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA
    GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA
    GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC
    CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131682.1
    CDS69..4616
    Translation

    Target ORF information:

    RefSeq Version XM_015276196.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276196.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT
    GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG
    TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA
    AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA
    GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC
    CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT
    GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT
    GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG
    TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA
    CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC
    AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG
    CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT
    CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC
    CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG
    CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT
    GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT
    CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA
    GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA
    GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA
    GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC
    CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35059
    Clone ID Related Accession (Same CDS sequence) XM_015276196.2 , XM_015276196.1
    Accession Version XM_015276196.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT
    GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG
    TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA
    AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA
    GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC
    CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT
    GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT
    GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG
    TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA
    CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC
    AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG
    CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT
    CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC
    CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG
    CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT
    GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT
    CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA
    GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA
    GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA
    GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC
    CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131682.1
    CDS29..4576
    Translation

    Target ORF information:

    RefSeq Version XM_015276196.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG TTCTACGTAT
    GAGTTTGAAG GCCCCACTAT CATCTACTGC CCTTCAAGAA AGGCCACTGA ACAAGTTATG
    TTTGAACTGA ATAAACTTGG TGTGACTTGT GGTGCATACC ATGCTGGCAT GGGAATTCAA
    AAAAGGAGAG ATACCCATCA CCAGTTCATG CGGGATGAAA TCCAGTGTGT TGTGGCTACA
    GTGGCATTTG GAATGGGCAT CAATAAAGCA GATATCCGGA TGGTTATTCA TTATGGTGCC
    CCCAAGGAAA TGGAATCCTA TTACCAAGAA ATTGGAAGAG CTGGTCGTGA TGGGCTTCCT
    GCTTCTTGTC ACGTCTTGTG GACTGCAGCA GACTTGGTTT TAAACAGGCG TCTTCTAAAT
    GAGATACACA ATGAAAAGTT CAGATTGTAC AAACTGAAGA TGTTAGAAAA AATGGAGAAG
    TACCTCTCCT CAAACAGCTG CAGAAGAAAA ATCATCCTGT CTCATTTTGA AGATAAACAA
    CTCCGAAAGG TCTCCTCTGG CATTATGGGC ACAGAAGAGT GCTGTGATAA TTGCAGGTGC
    AGAACCTCTT GTTTGGCAAC CTCCAGTGAT TCAGATGGTG GTTTACAGGA CTTTGGAAAG
    CAAGCATATC AGCTGCTTTC TGCAGTAAGC GCCCTGGAAG AGAAGTTTGG AACTAGAGTT
    CCCATTTTGT TTCTCCGAGG TTCTAATTCT CAGCGTCTGC CAGACAGATA CAGGAAACAC
    CCTCTGTACG GTAGTGGAAA AGACTGGACA GAGAACTGGT GGAAATCACT CAGCCAGCAG
    CTGATAATGG AAGGATTCCT CAGAGAAGTC TCTGGGCGTA GCAAGTTTGC AACAACGTGT
    GGGCTTACCC AGAAGGGTAG AAATTGGCTT TTGAAAGCCG GATCTGCCTC AAATCCAAGT
    CTTCTGTTAC AGTCCAATGA AGACTTAAAC CTACAGAGAA CTCCTAGAAG CAATAGGTCT
    TCACCTGCAG TCTCAGTGCA GCGCTCCCCA GACATGAAAG GATCAAAGCA GTCACCAGTT
    AAGCAGATGT CTTTGTATGA AATGTTTGCA CATGAGAAAA AAGCTGGAAC TCTTCCAAGA
    AGCAATACCA TGTTTAACAG CCCCCCAGAG CGCTCACCAG TGAAATCTCC TTTGAGGCCT
    CGAGTACCTG TTGTTTCATC CCGAGAAAAT GAACTAGAGA CTGCTCTCTA TGGCAAGTTG
    CTGACTGCTA GACAGAAGGT AGCTAATGAG AAGGTAATTC CTCCTGCTGT GTTGGCAACC
    AATAAGATCC TGGTGGAGAT GGCCCGAATG AGGCCTACCA CTGTTGAGAA TGTGAAGAGA
    ATTGATGGTG TATCTGAAGC CAAAGCGACG ATGTTGGTCC TTCTTCTAGC TGAGATTAAG
    GAGTTCTGCC AAGCAAATGG TTTGCAGACG GACATATTCC CCAAACCTGA ATCTACAGAT
    CAGAAAGAGA CATCTCCTTG GAAGAATACT AAAGCTCTGT CTCCCTCAGA GCATGTCACC
    TATGTCCTGT TCCAAGAGAA AAATCTCTCC CTGAGGACTA TCAGTGAGAC CAGATCTCTT
    CCTCTTACTG AGGTTGGCAC TCACTTGTTC CGGGCGGTGA AAGCTGGCTA TCCTGTCAAC
    CTGGAGCGAG CAGGTTTGAC TCCTGAAGTT CAACAGATCA TTTCTGATAT TATCCGTAAT
    CCTCCTATTG ACTCAGACAC CACCAAAATT CAAGCAATTA GAAAACTTGT TCCTGCACAT
    ATTGAACTGT ATCTCATCCA GATGGCTATT GTGTTGTTGG AGAAAAGGGA TGGCAGTAAA
    TCTAAGTATG GTTCAGGTGC CAAAAGGATG CTGGTGCTGT CAGACAAGCA GCAGGAAAAG
    ACAGGAGCAG ACCAAACAAG TAGGGGCAAA GCTTTGTGGA GTGGAAGAAA GGATGACTTG
    ACCAATCCTA TACTGAAGGA AGGAGATGAA AAGAACCATC CACAAGTGGT GCCCTCAGCT
    TTGCCTCCTT TGTTACTGTC CTATGGAAAG GCCACGTGGA GCACAGATGA TGCCCAGCCA
    GGTAACAGGG GTGCAGCAGA CCGCACGGAG CCCATCCAGT TGGCCTCCAG GAACCAGCCA
    GCCCTCAGCC CTGATGAAGA ACTGTTTGCT GACACTCAGC CAAAGAGCTC CCAGCCCCCA
    GGTAAGAGGA AGGTGCCTGC GTGGTTTGGA GCCTCAGCAG CCCCTGTTCT CCCTGTGCCC
    CAGACCAAGA AACCCAAAGC AGATACAAGA AGAGGGATCT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35060
    Clone ID Related Accession (Same CDS sequence) XM_015276197.1 , XM_015276197.2
    Accession Version XM_015276197.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC
    ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG
    TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG
    TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT
    ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131683.1
    CDS29..4552
    Translation

    Target ORF information:

    RefSeq Version XM_015276197.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC
    ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG
    TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG
    TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT
    ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35060
    Clone ID Related Accession (Same CDS sequence) XM_015276197.1 , XM_015276197.2
    Accession Version XM_015276197.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC
    ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG
    TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG
    TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT
    ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131683.1
    CDS69..4592
    Translation

    Target ORF information:

    RefSeq Version XM_015276197.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276197.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTCGGAA GGGGGGGCTG TTTTGCTGAC GCAGTTAGCG TGCGACGCGC GAGCCGGATG 
    GGCGCGGAGC CGGAGCCCGG TAACACGAAT GTGGATAAAA TGAGTAAAGT GGATTTGCAG
    GACAAGTATC CCCAGTGGAT GTGTGCACGT AACCAAGAGG GCATTTTGAG TGATAAGAAG
    ACACCTAACG TTCAGAGGAG CGTTTTGGAA GACGGGCTGC CGTTCCTAGC GTTCCGTGGC
    TCCATCGTTT ACAGCTATGA AGCCAGTGAC TGCTCCCTTC TCTCAGAAGA CATCAGGCAG
    ACGTTATCAG ATGGGGCTGC AGTTGGATTT GATATTGAAT GGCCGCCATC ATACACGAAA
    GGAAAAATGG CAAAAATAGC TCTAATCCAA CTATGTGTAA CCGAGGAGAA ATGTTATTTG
    TTTCACATCT CTTCCATGTC AGGTTTTCCC AAGGGACTCA AAAGGCTGCT GGAAGATGAA
    ACAATTAAAA AGGTTGGCGT AGGAATTGAG GGAGATCACT GGAAGCTGAT GGGTGACTTT
    GAAGTCAAAC TGAAGAGCTT TGTGGAGCTG GCTGACGTTG CTAATGAGAA ACTGAAGTGT
    AAGGAGGTAT GGAGTCTAAA TGGCTTGGTA AAACACCTCT TTGGGAAGCA GCTACTGAAA
    GATAAGTCTA TCCGCTGCGG CAACTGGGAA AAGTTTCCAC TCGATGAGGA GCAGAAATTG
    TATGCAGCCA CAGATGCTTA TGCTGGCTTC ATCATTTATC AAAAACTGAA AACTATGAAT
    AGCATTGACA GGAAATTATT TGGGGAAAGA AGAGATGAAG TGTTGTCAGA CGACGTGAAG
    GAGCGCTTAA CTTCGCTAGC TGAAGGGATG TCATACCTGG CTGCCAAGAT CTCTGACTCT
    TCTGGACAAC TCAGAAATTT GCAGAGGGCT GCAGAAATAC TAGCTGACAT ATCAGAGAAT
    GTAAAAACCT TAGGAAGTAC ACTCTTTGCT GGTTCAAGTT CTCCTTCTGT ACTGGAGAGG
    AGCTGTGGTG CAACTCCAAC AAAACTTGAA GCAAAGACAG TGAATGTTGA AGCACAAAAA
    GCTGAAATGC CTGAATTTGC TAAAAAACTT GAAGGTGATT TCAACATCTG CTCTGATGCT
    ACACTAGCTG GCCTCTGGGA GGGAGAAATT GATGAAGGTG AAGCAGAGGG AGGTAATGCT
    GTTGTTGAAG ATGGGGCTAC AGCAGCTGGC TCTAAGGACA CAGCAGACAG AGATGACTTC
    ATGTCGTTAG ACTTTAATGA GCAAGAACTA CAGATATTGG AACGTCAGGC TGCAGAAGAA
    GTTCTCAACA AAGCTGTGCC TGAGGAGGAA TGCCCAACAG ACAACGACCA AAATACCTCC
    TGTATCATTG AAAGTGATGA AGAACTGGAA ATGGAGATGC TTAAATCTTT AGAAGATGTG
    GACAATTCTA AAGAAGTTCC AACTGAAAGG GAGTCCAGCA AAGCCAGGAA AACTCCGGAC
    ACAGAATTGA ACGTCGCACC AGATGGGGAT GAAGATGAAG TCATTGAAGA GGATGAGGAG
    GAATGTTGGG ATCCATTGCT GTCAGTGCCT AGTGAAATCC ACATCGCATG CCTGAAGACA
    TATTTTGGGC ATTCTTCTTT TAAACCGGTT CAGTGGAAAG TGATACATTC TGTTTTAGAA
    GACAAAAGAG ACAATCTTGT TGTCATGGCA ACTGGGTATG GGAAGAGTTT GTGCTACCAG
    TTTCCTCCTG TTTACACTGG AGGTACAGGA ATAGTCATCT GTCCTCTTAT TTCCTTGATG
    GAGGACCAAG TTCTGCAACT GACAATGTCA GGGATTTCAT CTTGCTTGCT TGGTTCAGCT
    CAGTCAAAAG ATGTCAAAGA CAACATTAAA GCGGGTCTAT ACAGAGTCAT ATACATGACA
    CCAGAGTTCT GTTCAGGGAA TTTAGAGCTA CTTCAGGATA TTGACCGAAC TACTGGTATT
    GCTCTCATAG CTGTTGATGA AGCTCACTGC ATTTCTGAGT GGGGCCATGA CTTCAGAATT
    TCCTTTAGAA AGTTAAACTC ATTGAAGAAG GCTCTACCAT CGGTTCCCAT TGTAGCATTA
    ACAGCAACTG CAAGTCCCTC AATTAGAGAA GACATAGTGA ATTGTCTGAA CCTAAAGAAT
    CCTCAGGTCA CATGTACTAG CTTTGACAGA CCTAATCTGT ACTTAGAAGT TGGGCGACAG
    AGTGGAAATA CCCTTAGGGA TTTGAAGCAG TTCCTTACTA GGAAAGGAAG CAGTTCTACG
    TATGAGTTTG AAGGCCCCAC TATCATCTAC TGCCCTTCAA GAAAGGCCAC TGAACAAGTT
    ATGTTTGAAC TGAATAAACT TGGTGTGACT TGTGGTGCAT ACCATGCTGG CATGGGAATT
    CAAAAAAGGA GAGATACCCA TCACCAGTTC ATGCGGGATG AAATCCAGTG TGTTGTGGCT
    ACAGTGGCAT TTGGAATGGG CATCAATAAA GCAGATATCC GGATGGTTAT TCATTATGGT
    GCCCCCAAGG AAATGGAATC CTATTACCAA GAAATTGGAA GAGCTGGTCG TGATGGGCTT
    CCTGCTTCTT GTCACGTCTT GTGGACTGCA GCAGACTTGG TTTTAAACAG GCGTCTTCTA
    AATGAGATAC ACAATGAAAA GTTCAGATTG TACAAACTGA AGATGTTAGA AAAAATGGAG
    AAGTACCTCT CCTCAAACAG CTGCAGAAGA AAAATCATCC TGTCTCATTT TGAAGATAAA
    CAACTCCGAA AGGTCTCCTC TGGCATTATG GGCACAGAAG AGTGCTGTGA TAATTGCAGG
    TGCAGAACCT CTTGTTTGGC AACCTCCAGT GATTCAGATG GTGGTTTACA GGACTTTGGA
    AAGCAAGCAT ATCAGCTGCT TTCTGCAGTA AGCGCCCTGG AAGAGAAGTT TGGAACTAGA
    GTTCCCATTT TGTTTCTCCG AGGTTCTAAT TCTCAGCGTC TGCCAGACAG ATACAGGAAA
    CACCCTCTGT ACGGTAGTGG AAAAGACTGG ACAGAGAACT GGTGGAAATC ACTCAGCCAG
    CAGCTGATAA TGGAAGGATT CCTCAGAGAA GTCTCTGGGC GTAGCAAGTT TGCAACAACG
    TGTGGGCTTA CCCAGAAGGG TAGAAATTGG CTTTTGAAAG CCGGATCTGC CTCAAATCCA
    AGTCTTCTGT TACAGTCCAA TGAAGACTTA AACCTACAGA GAACTCCTAG AAGCAATAGG
    TCTTCACCTG CAGTCTCAGT GCAGCGCTCC CCAGACATGA AAGGATCAAA GCAGTCACCA
    GTTAAGCAGA TGTCTTTGTA TGAAATGTTT GCACATGAGA AAAAAGCTGG AACTCTTCCA
    AGAAGCAATA CCATGTTTAA CAGCCCCCCA GAGCGCTCAC CAGTGAAATC TCCTTTGAGG
    CCTCGAGTAC CTGTTGTTTC ATCCCGAGAA AATGAACTAG AGACTGCTCT CTATGGCAAG
    TTGCTGACTG CTAGACAGAA GGTAGCTAAT GAGAAGGTAA TTCCTCCTGC TGTGTTGGCA
    ACCAATAAGA TCCTGGTGGA GATGGCCCGA ATGAGGCCTA CCACTGTTGA GAATGTGAAG
    AGAATTGATG GTGTATCTGA AGCCAAAGCG ACGATGTTGG TCCTTCTTCT AGCTGAGATT
    AAGGAGTTCT GCCAAGCAAA TGGTTTGCAG ACGGACATAT TCCCCAAACC TGAATCTACA
    GATCAGAAAG AGACATCTCC TTGGAAGAAT ACTAAAGCTC TGTCTCCCTC AGAGCATGTC
    ACCTATGTCC TGTTCCAAGA GAAAAATCTC TCCCTGAGGA CTATCAGTGA GACCAGATCT
    CTTCCTCTTA CTGAGGTTGG CACTCACTTG TTCCGGGCGG TGAAAGCTGG CTATCCTGTC
    AACCTGGAGC GAGCAGGTTT GACTCCTGAA GTTCAACAGA TCATTTCTGA TATTATCCGT
    AATCCTCCTA TTGACTCAGA CACCACCAAA ATTCAAGCAA TTAGAAAACT TGTTCCTGCA
    CATATTGAAC TGTATCTCAT CCAGATGGCT ATTGTGTTGT TGGAGAAAAG GGATGGCAGT
    AAATCTAAGT ATGGTTCAGG TGCCAAAAGG ATGCTGGTGC TGTCAGACAA GCAGCAGGAA
    AAGACAGGAG CAGACCAAAC AAGTAGGGGC AAAGCTTTGT GGAGTGGAAG AAAGGATGAC
    TTGACCAATC CTATACTGAA GGAAGGAGAT GAAAAGAACC ATCCACAAGT GGTGCCCTCA
    GCTTTGGCCA CGTGGAGCAC AGATGATGCC CAGCCAGGTA ACAGGGGTGC AGCAGACCGC
    ACGGAGCCCA TCCAGTTGGC CTCCAGGAAC CAGCCAGCCC TCAGCCCTGA TGAAGAACTG
    TTTGCTGACA CTCAGCCAAA GAGCTCCCAG CCCCCAGGTA AGAGGAAGGT GCCTGCGTGG
    TTTGGAGCCT CAGCAGCCCC TGTTCTCCCT GTGCCCCAGA CCAAGAAACC CAAAGCAGAT
    ACAAGAAGAG GGATCTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35061
    Clone ID Related Accession (Same CDS sequence) XM_015276198.1 , XM_015276198.2
    Accession Version XM_015276198.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
    TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC
    TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG
    ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG
    CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT
    CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA
    TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA
    ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC
    AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC
    TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT
    GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC
    TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA
    CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT
    CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA
    GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG
    GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA
    CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT
    TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA
    GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT
    TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT
    GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC
    ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG
    GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA
    GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG
    CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC
    AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA
    GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC
    CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA
    GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT
    GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC
    TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA
    TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA
    TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT
    ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG
    TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA
    TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG
    AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG
    TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT
    AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA
    AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA
    TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT
    GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC
    CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA
    AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG
    GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG
    AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC
    CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA
    GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT
    GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG
    GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT
    CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC
    TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG
    CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA
    GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG
    AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG
    AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG
    AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA
    CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA
    GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA
    ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT
    ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG
    GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA
    TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT
    CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG
    ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG
    GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG
    ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA
    ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG
    TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG
    CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG
    TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC
    CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG
    TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC
    CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT
    CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA
    GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG
    ATCTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131684.1
    CDS142..4713
    Translation

    Target ORF information:

    RefSeq Version XM_015276198.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
    TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC
    TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG
    ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG
    CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT
    CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA
    TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA
    ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC
    AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC
    TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT
    GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC
    TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA
    CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT
    CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA
    GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG
    GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA
    CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT
    TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA
    GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT
    TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT
    GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC
    ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG
    GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA
    GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG
    CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC
    AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA
    GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC
    CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA
    GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT
    GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC
    TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA
    TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA
    TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT
    ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG
    TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA
    TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG
    AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG
    TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT
    AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA
    AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA
    TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT
    GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC
    CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA
    AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG
    GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG
    AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC
    CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA
    GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT
    GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG
    GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT
    CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC
    TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG
    CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA
    GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG
    AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG
    AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG
    AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA
    CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA
    GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA
    ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT
    ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG
    GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA
    TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT
    CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG
    ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG
    GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG
    ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA
    ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG
    TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG
    CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG
    TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC
    CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG
    TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC
    CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT
    CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA
    GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG
    ATCTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35061
    Clone ID Related Accession (Same CDS sequence) XM_015276198.1 , XM_015276198.2
    Accession Version XM_015276198.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
    TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC
    TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG
    ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG
    CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT
    CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA
    TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA
    ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC
    AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC
    TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT
    GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC
    TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA
    CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT
    CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA
    GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG
    GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA
    CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT
    TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA
    GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT
    TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT
    GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC
    ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG
    GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA
    GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG
    CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC
    AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA
    GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC
    CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA
    GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT
    GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC
    TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA
    TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA
    TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT
    ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG
    TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA
    TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG
    AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG
    TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT
    AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA
    AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA
    TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT
    GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC
    CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA
    AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG
    GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG
    AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC
    CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA
    GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT
    GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG
    GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT
    CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC
    TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG
    CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA
    GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG
    AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG
    AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG
    AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA
    CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA
    GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA
    ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT
    ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG
    GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA
    TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT
    CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG
    ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG
    GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG
    ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA
    ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG
    TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG
    CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG
    TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC
    CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG
    TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC
    CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT
    CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA
    GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG
    ATCTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131684.1
    CDS129..4700
    Translation

    Target ORF information:

    RefSeq Version XM_015276198.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276198.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCTGCTCT TGATGCAGCC CAAGATACTG TTGGCCTTCT GGGCTGGAAG CACACGCTGC 
    TGGCTCGTGT TGAATCTTTC ATCAACCAGC ACTCCCAGAT TCTTCTCCTC AGGGCTGCTC
    TCAAGCCATT CTCCACCCAG CCTGTATCTC TGCTTGGGAT TTCCCCAACC CAGGTTCAGG
    ACCTTGCACC TTGACCTTCT AACACCTAAC GTTCAGAGGA GCGTTTTGGA AGACGGGCTG
    CCGTTCCTAG CGTTCCGTGG CTCCATCGTT TACAGCTATG AAGCCAGTGA CTGCTCCCTT
    CTCTCAGAAG ACATCAGGCA GACGTTATCA GATGGGGCTG CAGTTGGATT TGATATTGAA
    TGGCCGCCAT CATACACGAA AGGAAAAATG GCAAAAATAG CTCTAATCCA ACTATGTGTA
    ACCGAGGAGA AATGTTATTT GTTTCACATC TCTTCCATGT CAGGTTTTCC CAAGGGACTC
    AAAAGGCTGC TGGAAGATGA AACAATTAAA AAGGTTGGCG TAGGAATTGA GGGAGATCAC
    TGGAAGCTGA TGGGTGACTT TGAAGTCAAA CTGAAGAGCT TTGTGGAGCT GGCTGACGTT
    GCTAATGAGA AACTGAAGTG TAAGGAGGTA TGGAGTCTAA ATGGCTTGGT AAAACACCTC
    TTTGGGAAGC AGCTACTGAA AGATAAGTCT ATCCGCTGCG GCAACTGGGA AAAGTTTCCA
    CTCGATGAGG AGCAGAAATT GTATGCAGCC ACAGATGCTT ATGCTGGCTT CATCATTTAT
    CAAAAACTGA AAACTATGAA TAGCATTGAC AGGAAATTAT TTGGGGAAAG AAGAGATGAA
    GTGTTGTCAG ACGACGTGAA GGAGCGCTTA ACTTCGCTAG CTGAAGGGAT GTCATACCTG
    GCTGCCAAGA TCTCTGACTC TTCTGGACAA CTCAGAAATT TGCAGAGGGC TGCAGAAATA
    CTAGCTGACA TATCAGAGAA TGTAAAAACC TTAGGAAGTA CACTCTTTGC TGGTTCAAGT
    TCTCCTTCTG TACTGGAGAG GAGCTGTGGT GCAACTCCAA CAAAACTTGA AGCAAAGACA
    GTGAATGTTG AAGCACAAAA AGCTGAAATG CCTGAATTTG CTAAAAAACT TGAAGGTGAT
    TTCAACATCT GCTCTGATGC TACACTAGCT GGCCTCTGGG AGGGAGAAAT TGATGAAGGT
    GAAGCAGAGG GAGGTAATGC TGTTGTTGAA GATGGGGCTA CAGCAGCTGG CTCTAAGGAC
    ACAGCAGACA GAGATGACTT CATGTCGTTA GACTTTAATG AGCAAGAACT ACAGATATTG
    GAACGTCAGG CTGCAGAAGA AGTTCTCAAC AAAGCTGTGC CTGAGGAGGA ATGCCCAACA
    GACAACGACC AAAATACCTC CTGTATCATT GAAAGTGATG AAGAACTGGA AATGGAGATG
    CTTAAATCTT TAGAAGATGT GGACAATTCT AAAGAAGTTC CAACTGAAAG GGAGTCCAGC
    AAAGCCAGGA AAACTCCGGA CACAGAATTG AACGTCGCAC CAGATGGGGA TGAAGATGAA
    GTCATTGAAG AGGATGAGGA GGAATGTTGG GATCCATTGC TGTCAGTGCC TAGTGAAATC
    CACATCGCAT GCCTGAAGAC ATATTTTGGG CATTCTTCTT TTAAACCGGT TCAGTGGAAA
    GTGATACATT CTGTTTTAGA AGACAAAAGA GACAATCTTG TTGTCATGGC AACTGGGTAT
    GGGAAGAGTT TGTGCTACCA GTTTCCTCCT GTTTACACTG GAGGTACAGG AATAGTCATC
    TGTCCTCTTA TTTCCTTGAT GGAGGACCAA GTTCTGCAAC TGACAATGTC AGGGATTTCA
    TCTTGCTTGC TTGGTTCAGC TCAGTCAAAA GATGTCAAAG ACAACATTAA AGCGGGTCTA
    TACAGAGTCA TATACATGAC ACCAGAGTTC TGTTCAGGGA ATTTAGAGCT ACTTCAGGAT
    ATTGACCGAA CTACTGGTAT TGCTCTCATA GCTGTTGATG AAGCTCACTG CATTTCTGAG
    TGGGGCCATG ACTTCAGAAT TTCCTTTAGA AAGTTAAACT CATTGAAGAA GGCTCTACCA
    TCGGTTCCCA TTGTAGCATT AACAGCAACT GCAAGTCCCT CAATTAGAGA AGACATAGTG
    AATTGTCTGA ACCTAAAGAA TCCTCAGGTC ACATGTACTA GCTTTGACAG ACCTAATCTG
    TACTTAGAAG TTGGGCGACA GAGTGGAAAT ACCCTTAGGG ATTTGAAGCA GTTCCTTACT
    AGGAAAGGAA GCAGTTCTAC GTATGAGTTT GAAGGCCCCA CTATCATCTA CTGCCCTTCA
    AGAAAGGCCA CTGAACAAGT TATGTTTGAA CTGAATAAAC TTGGTGTGAC TTGTGGTGCA
    TACCATGCTG GCATGGGAAT TCAAAAAAGG AGAGATACCC ATCACCAGTT CATGCGGGAT
    GAAATCCAGT GTGTTGTGGC TACAGTGGCA TTTGGAATGG GCATCAATAA AGCAGATATC
    CGGATGGTTA TTCATTATGG TGCCCCCAAG GAAATGGAAT CCTATTACCA AGAAATTGGA
    AGAGCTGGTC GTGATGGGCT TCCTGCTTCT TGTCACGTCT TGTGGACTGC AGCAGACTTG
    GTTTTAAACA GGCGTCTTCT AAATGAGATA CACAATGAAA AGTTCAGATT GTACAAACTG
    AAGATGTTAG AAAAAATGGA GAAGTACCTC TCCTCAAACA GCTGCAGAAG AAAAATCATC
    CTGTCTCATT TTGAAGATAA ACAACTCCGA AAGGTCTCCT CTGGCATTAT GGGCACAGAA
    GAGTGCTGTG ATAATTGCAG GTGCAGAACC TCTTGTTTGG CAACCTCCAG TGATTCAGAT
    GGTGGTTTAC AGGACTTTGG AAAGCAAGCA TATCAGCTGC TTTCTGCAGT AAGCGCCCTG
    GAAGAGAAGT TTGGAACTAG AGTTCCCATT TTGTTTCTCC GAGGTTCTAA TTCTCAGCGT
    CTGCCAGACA GATACAGGAA ACACCCTCTG TACGGTAGTG GAAAAGACTG GACAGAGAAC
    TGGTGGAAAT CACTCAGCCA GCAGCTGATA ATGGAAGGAT TCCTCAGAGA AGTCTCTGGG
    CGTAGCAAGT TTGCAACAAC GTGTGGGCTT ACCCAGAAGG GTAGAAATTG GCTTTTGAAA
    GCCGGATCTG CCTCAAATCC AAGTCTTCTG TTACAGTCCA ATGAAGACTT AAACCTACAG
    AGAACTCCTA GAAGCAATAG GTCTTCACCT GCAGTCTCAG TGCAGCGCTC CCCAGACATG
    AAAGGATCAA AGCAGTCACC AGTTAAGCAG ATGTCTTTGT ATGAAATGTT TGCACATGAG
    AAAAAAGCTG GAACTCTTCC AAGAAGCAAT ACCATGTTTA ACAGCCCCCC AGAGCGCTCA
    CCAGTGAAAT CTCCTTTGAG GCCTCGAGTA CCTGTTGTTT CATCCCGAGA AAATGAACTA
    GAGACTGCTC TCTATGGCAA GTTGCTGACT GCTAGACAGA AGGTAGCTAA TGAGAAGGTA
    ATTCCTCCTG CTGTGTTGGC AACCAATAAG ATCCTGGTGG AGATGGCCCG AATGAGGCCT
    ACCACTGTTG AGAATGTGAA GAGAATTGAT GGTGTATCTG AAGCCAAAGC GACGATGTTG
    GTCCTTCTTC TAGCTGAGAT TAAGGAGTTC TGCCAAGCAA ATGGTTTGCA GACGGACATA
    TTCCCCAAAC CTGAATCTAC AGATCAGAAA GAGACATCTC CTTGGAAGAA TACTAAAGCT
    CTGTCTCCCT CAGAGCATGT CACCTATGTC CTGTTCCAAG AGAAAAATCT CTCCCTGAGG
    ACTATCAGTG AGACCAGATC TCTTCCTCTT ACTGAGGTTG GCACTCACTT GTTCCGGGCG
    GTGAAAGCTG GCTATCCTGT CAACCTGGAG CGAGCAGGTT TGACTCCTGA AGTTCAACAG
    ATCATTTCTG ATATTATCCG TAATCCTCCT ATTGACTCAG ACACCACCAA AATTCAAGCA
    ATTAGAAAAC TTGTTCCTGC ACATATTGAA CTGTATCTCA TCCAGATGGC TATTGTGTTG
    TTGGAGAAAA GGGATGGCAG TAAATCTAAG TATGGTTCAG GTGCCAAAAG GATGCTGGTG
    CTGTCAGACA AGCAGCAGGA AAAGACAGGA GCAGACCAAA CAAGTAGGGG CAAAGCTTTG
    TGGAGTGGAA GAAAGGATGA CTTGACCAAT CCTATACTGA AGGAAGGAGA TGAAAAGAAC
    CATCCACAAG TGGTGCCCTC AGCTTTGCCT CCTTTGTTAC TGTCCTATGG AAAGGCCACG
    TGGAGCACAG ATGATGCCCA GCCAGGTAAC AGGGGTGCAG CAGACCGCAC GGAGCCCATC
    CAGTTGGCCT CCAGGAACCA GCCAGCCCTC AGCCCTGATG AAGAACTGTT TGCTGACACT
    CAGCCAAAGA GCTCCCAGCC CCCAGGTAAG AGGAAGGTGC CTGCGTGGTT TGGAGCCTCA
    GCAGCCCCTG TTCTCCCTGT GCCCCAGACC AAGAAACCCA AAGCAGATAC AAGAAGAGGG
    ATCTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28582
    Clone ID Related Accession (Same CDS sequence) NM_001282289.3
    Accession Version NM_001282289.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-17
    Organism Gallus gallus(chicken)
    Product Werner syndrome ATP-dependent helicase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000017.1. On Feb 11, 2016 this sequence version replaced NM_001282289.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB035866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201366, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
    CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG
    AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT
    GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG
    CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG
    AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT
    TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT
    GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC
    TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG
    TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG
    AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA
    TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG
    AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG
    AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC
    TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG
    AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG
    AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA
    AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT
    GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT
    GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC
    TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA
    GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC
    TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT
    GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG
    GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG
    GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG
    ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA
    GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC
    CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG
    ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA
    GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG
    ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT
    ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA
    ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA
    TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG
    AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA
    CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT
    ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA
    GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA
    ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG
    GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT
    GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG
    CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT
    CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG
    GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT
    AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC
    AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT
    GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT
    AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG
    AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC
    CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA
    ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT
    CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT
    AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA
    CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT
    CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG
    AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC
    AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG
    GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG
    AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG
    ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT
    ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT
    GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA
    TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT
    GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC
    CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT
    GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC
    AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG
    GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT
    GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC
    TCAGCTTTGG CCACGTGGAG CACAGATGAT GCCCAGCCAG GTAACAGGGG TGCAGCAGAC
    CGCACGGAGC CCATCCAGTT GGCCTCCAGG AACCAGCCAG CCCTCAGCCC TGATGAAGAA
    CTGTTTGCTG ACACTCAGCC AAAGAGCTCC CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG
    TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC CCTGTGCCCC AGACCAAGAA ACCCAAAGCA
    GATACAAGAA GAGGGATCTT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269218.2
    CDS99..4565
    Translation

    Target ORF information:

    RefSeq Version NM_001282289.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Werner syndrome RecQ like helicase (WRN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282289.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTGAAGG TGGCCCGCCC AGGTAACACG AATGTGGATA AAATGAGTAA AGTGGATTTG 
    CAGGACAAGT ATCCCCAGTG GATGTGTGCA CGTAACCAAG AGGGCATTTT GAGTGATAAG
    AAGACACCTA ACGTTCAGAG GAGCGTTTTG GAAGACGGGC TGCCGTTCCT AGCGTTCCGT
    GGCTCCATCG TTTACAGCTA TGAAGCCAGT GACTGCTCCC TTCTCTCAGA AGACATCAGG
    CAGACGTTAT CAGATGGGGC TGCAGTTGGA TTTGATATTG AATGGCCGCC ATCATACACG
    AAAGGAAAAA TGGCAAAAAT AGCTCTAATC CAACTATGTG TAACCGAGGA GAAATGTTAT
    TTGTTTCACA TCTCTTCCAT GTCAGGTTTT CCCAAGGGAC TCAAAAGGCT GCTGGAAGAT
    GAAACAATTA AAAAGGTTGG CGTAGGAATT GAGGGAGATC ACTGGAAGCT GATGGGTGAC
    TTTGAAGTCA AACTGAAGAG CTTTGTGGAG CTGGCTGACG TTGCTAATGA GAAACTGAAG
    TGTAAGGAGG TATGGAGTCT AAATGGCTTG GTAAAACACC TCTTTGGGAA GCAGCTACTG
    AAAGATAAGT CTATCCGCTG CGGCAACTGG GAAAAGTTTC CACTCGATGA GGAGCAGAAA
    TTGTATGCAG CCACAGATGC TTATGCTGGC TTCATCATTT ATCAAAAACT GAAAACTATG
    AATAGCATTG ACAGGAAATT ATTTGGGGAA AGAAGAGATG AAGTGTTGTC AGACGACGTG
    AAGGAGCGCT TAACTTCGCT AGCTGAAGGG ATGTCATACC TGGCTGCCAA GATCTCTGAC
    TCTTCTGGAC AACTCAGAAA TTTGCAGAGG GCTGCAGAAA TACTAGCTGA CATATCAGAG
    AATGTAAAAA CCTTAGGAAG TACACTCTTT GCTGGTTCAA GTTCTCCTTC TGTACTGGAG
    AGGAGCTGTG GTGCAACTCC AACAAAACTT GAAGCAAAGA CAGTGAATGT TGAAGCACAA
    AAAGCTGAAA TGCCTGAATT TGCTAAAAAA CTTGAAGGTG ATTTCAACAT CTGCTCTGAT
    GCTACACTAG CTGGCCTCTG GGAGGGAGAA ATTGATGAAG GTGAAGCAGA GGGAGGTAAT
    GCTGTTGTTG AAGATGGGGC TACAGCAGCT GGCTCTAAGG ACACAGCAGA CAGAGATGAC
    TTCATGTCGT TAGACTTTAA TGAGCAAGAA CTACAGATAT TGGAACGTCA GGCTGCAGAA
    GAAGTTCTCA ACAAAGCTGT GCCTGAGGAG GAATGCCCAA CAGACAACGA CCAAAATACC
    TCCTGTATCA TTGAAAGTGA TGAAGAACTG GAAATGGAGA TGCTTAAATC TTTAGAAGAT
    GTGGACAATT CTAAAGAAGT TCCAACTGAA AGGGAGTCCA GCAAAGCCAG GAAAACTCCG
    GACACAGAAT TGAACGTCGC ACCAGATGGG GATGAAGATG AAGTCATTGA AGAGGATGAG
    GAGGAATGTT GGGATCCATT GCTGTCAGTG CCTAGTGAAA TCCACATCGC ATGCCTGAAG
    ACATATTTTG GGCATTCTTC TTTTAAACCG GTTCAGTGGA AAGTGATACA TTCTGTTTTA
    GAAGACAAAA GAGACAATCT TGTTGTCATG GCAACTGGGT ATGGGAAGAG TTTGTGCTAC
    CAGTTTCCTC CTGTTTACAC TGGAGGTACA GGAATAGTCA TCTGTCCTCT TATTTCCTTG
    ATGGAGGACC AAGTTCTGCA ACTGACAATG TCAGGGATTT CATCTTGCTT GCTTGGTTCA
    GCTCAGTCAA AAGATGTCAA AGACAACATT AAAGCGGGTC TATACAGAGT CATATACATG
    ACACCAGAGT TCTGTTCAGG GAATTTAGAG CTACTTCAGG ATATTGACCG AACTACTGGT
    ATTGCTCTCA TAGCTGTTGA TGAAGCTCAC TGCATTTCTG AGTGGGGCCA TGACTTCAGA
    ATTTCCTTTA GAAAGTTAAA CTCATTGAAG AAGGCTCTAC CATCGGTTCC CATTGTAGCA
    TTAACAGCAA CTGCAAGTCC CTCAATTAGA GAAGACATAG TGAATTGTCT GAACCTAAAG
    AATCCTCAGG TCACATGTAC TAGCTTTGAC AGACCTAATC TGTACTTAGA AGTTGGGCGA
    CAGAGTGGAA ATACCCTTAG GGATTTGAAG CAGTTCCTTA CTAGGAAAGG AAGCAGTTCT
    ACGTATGAGT TTGAAGGCCC CACTATCATC TACTGCCCTT CAAGAAAGGC CACTGAACAA
    GTTATGTTTG AACTGAATAA ACTTGGTGTG ACTTGTGGTG CATACCATGC TGGCATGGGA
    ATTCAAAAAA GGAGAGATAC CCATCACCAG TTCATGCGGG ATGAAATCCA GTGTGTTGTG
    GCTACAGTGG CATTTGGAAT GGGCATCAAT AAAGCAGATA TCCGGATGGT TATTCATTAT
    GGTGCCCCCA AGGAAATGGA ATCCTATTAC CAAGAAATTG GAAGAGCTGG TCGTGATGGG
    CTTCCTGCTT CTTGTCACGT CTTGTGGACT GCAGCAGACT TGGTTTTAAA CAGGCGTCTT
    CTAAATGAGA TACACAATGA AAAGTTCAGA TTGTACAAAC TGAAGATGTT AGAAAAAATG
    GAGAAGTACC TCTCCTCAAA CAGCTGCAGA AGAAAAATCA TCCTGTCTCA TTTTGAAGAT
    AAACAACTCC GAAAGGTCTC CTCTGGCATT ATGGGCACAG AAGAGTGCTG TGATAATTGC
    AGGTGCAGAA CCTCTTGTTT GGCAACCTCC AGTGATTCAG ATGGTGGTTT ACAGGACTTT
    GGAAAGCAAG CATATCAGCT GCTTTCTGCA GTAAGCGCCC TGGAAGAGAA GTTTGGAACT
    AGAGTTCCCA TTTTGTTTCT CCGAGGTTCT AATTCTCAGC GTCTGCCAGA CAGATACAGG
    AAACACCCTC TGTACGGTAG TGGAAAAGAC TGGACAGAGA ACTGGTGGAA ATCACTCAGC
    CAGCAGCTGA TAATGGAAGG ATTCCTCAGA GAAGTCTCTG GGCGTAGCAA GTTTGCAACA
    ACGTGTGGGC TTACCCAGAA GGGTAGAAAT TGGCTTTTGA AAGCCGGATC TGCCTCAAAT
    CCAAGTCTTC TGTTACAGTC CAATGAAGAC TTAAACCTAC AGAGAACTCC TAGAAGCAAT
    AGGTCTTCAC CTGCAGTCTC AGTGCAGCGC TCCCCAGACA TGAAAGGATC AAAGCAGTCA
    CCAGTTAAGC AGATGTCTTT GTATGAAATG TTTGCACATG AGAAAAAAGC TGGAACTCTT
    CCAAGAAGCA ATACCATGTT TAACAGCCCC CCAGAGCGCT CACCAGTGAA ATCTCCTTTG
    AGGCCTCGAG TACCTGTTGT TTCATCCCGA GAAAATGAAC TAGAGACTGC TCTCTATGGC
    AAGTTGCTGA CTGCTAGACA GAAGGTAGCT AATGAGAAGG TAATTCCTCC TGCTGTGTTG
    GCAACCAATA AGATCCTGGT GGAGATGGCC CGAATGAGGC CTACCACTGT TGAGAATGTG
    AAGAGAATTG ATGGTGTATC TGAAGCCAAA GCGACGATGT TGGTCCTTCT TCTAGCTGAG
    ATTAAGGAGT TCTGCCAAGC AAATGGTTTG CAGACGGACA TATTCCCCAA ACCTGAATCT
    ACAGATCAGA AAGAGACATC TCCTTGGAAG AATACTAAAG CTCTGTCTCC CTCAGAGCAT
    GTCACCTATG TCCTGTTCCA AGAGAAAAAT CTCTCCCTGA GGACTATCAG TGAGACCAGA
    TCTCTTCCTC TTACTGAGGT TGGCACTCAC TTGTTCCGGG CGGTGAAAGC TGGCTATCCT
    GTCAACCTGG AGCGAGCAGG TTTGACTCCT GAAGTTCAAC AGATCATTTC TGATATTATC
    CGTAATCCTC CTATTGACTC AGACACCACC AAAATTCAAG CAATTAGAAA ACTTGTTCCT
    GCACATATTG AACTGTATCT CATCCAGATG GCTATTGTGT TGTTGGAGAA AAGGGATGGC
    AGTAAATCTA AGTATGGTTC AGGTGCCAAA AGGATGCTGG TGCTGTCAGA CAAGCAGCAG
    GAAAAGACAG GAGCAGACCA AACAAGTAGG GGCAAAGCTT TGTGGAGTGG AAGAAAGGAT
    GACTTGACCA ATCCTATACT GAAGGAAGGA GATGAAAAGA ACCATCCACA AGTGGTGCCC
    TCAGCTTTGG CCACGTGGAG CACAGATGAT GCCCAGCCAG GTAACAGGGG TGCAGCAGAC
    CGCACGGAGC CCATCCAGTT GGCCTCCAGG AACCAGCCAG CCCTCAGCCC TGATGAAGAA
    CTGTTTGCTG ACACTCAGCC AAAGAGCTCC CAGCCCCCAG GTAAGAGGAA GGTGCCTGCG
    TGGTTTGGAG CCTCAGCAGC CCCTGTTCTC CCTGTGCCCC AGACCAAGAA ACCCAAAGCA
    GATACAAGAA GAGGGATCTT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.