MIA3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIA3
Entrez Gene ID	421337
Full Name	MIA family member 3, ER export factor
General protein information	Preferred Names MIA family member 3, ER export factor Names melanoma inhibitory activity protein 3 melanoma inhibitory activity family, member 3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001277558.2	NP_001264487.1	melanoma inhibitory activity protein 3 precursor
XM_015283644.1	XP_015139130.1	melanoma inhibitory activity protein 3 isoform X2
XM_015283643.2	XP_015139129.2	transport and Golgi organization protein 1 homolog isoform X1
XM_015283644.2	XP_015139130.2	transport and Golgi organization protein 1 homolog isoform X2
XM_015283643.1	XP_015139129.1	melanoma inhibitory activity protein 3 isoform X1
NM_001277558.3	NP_001264487.1	transport and Golgi organization protein 1 homolog precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MIA3	XP_514216.4
Gallus gallus (chicken)	MIA3	NP_001264487.1

Gene Ontology
Bibliography

Related articles in PubMed

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Carre W, Wang X, Porter TE, Nys Y, Tang J, Bernberg E, Morgan R, Burnside J, Aggrey SE, Simon J, Cogburn LA
Physiological genomics25(3)514-24(2006 May)

Expressed sequence tag analysis of Eimeria-stimulated intestinal intraepithelial lymphocytes in chickens.
Min W, Lillehoj HS, Ashwell CM, van Tassell CP, Dalloul RA, Matukumalli LK, Han JY, Lillehoj EP
Molecular biotechnology30(2)143-50(2005 Jun)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

An expressed sequence tag database of T-cell-enriched activated chicken splenocytes: sequence analysis of 5251 clones.
Tirunagaru VG, Sofer L, Cui J, Burnside J
Genomics66(2)144-51(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MIA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa148630	XM_015283643.2 Latest version!	Gallus gallus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa148631	XM_015283644.2 Latest version!	Gallus gallus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa25229	NM_001277558.3 Latest version!	Gallus gallus MIA family member 3, ER export factor (MIA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33332	XM_015283643.1 Latest version!	Gallus gallus melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OGa33333	XM_015283644.1 Latest version!	Gallus gallus melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OGa25229	NM_001277558.2	Gallus gallus MIA family member 3, ER export factor (MIA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIA3 ( XM_015283643.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139129.2 Gallus gallus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa148630
Clone ID Related Accession (Same CDS sequence)	XM_015283643.2
Accession Version	XM_015283643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transport and Golgi organization protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCAGCCCT CTCCCAAAGA ACCTGAAGCC 
CCCCGTGTAA CTACCGATTC ACCTTCATCT ATTGAACCAG CTACAGTTAA CGTCGGTCCC 
AAAGGCCCAC CTTCTTTCCC TGGGACGCCC GTCATGACCT CTCCAGTGCT GGGACCTCCA 
CCTCCACCAC CTGTTAACTA TGGTCCACCA CCAGCTCCCT TTCCTGGACA CTACGGGCCT 
GGGCCTCGAC CTCTCCCTGT GCCCCTAGTT TGTGGTGCTC CCTTACCACC ACCAGCTGCA 
AGAGATTTCT TGCCTGGCCC ATCCTTAGGA ATAAGAGATC TGCCTCCTGG GCCACTGCCA 
CCCCTCCCAG ACCCCAGAAG CTACAGACGT GGGCACCCTC ATTTCCGGCC TCCAGGTCCT 
CCTGGCCCAC GTGATTATCC GCCAGGCCCG CCGCTACCAC CGCCTGCCTC CAGGGACTAC 
GCACCTTCTC GGAGCAGAGA CTTGCCTCCA GCAGGACCCA GAGACTACCC CGCAGGCCCC 
GCACCGCCCC CGGCAGGCTC AAAAGACTAC GCACAGCCTC CAGTGCAGAA GCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139129.2
CDS	45..6101
Translation	MAAAAPPDPRLLLAVLLLLPPPGPLCAAGPELSRRFTERKRCADPECSKLMCRGKAMKDFQGPDCRFVNFKKGETVYVYYKLIGMSTELWAGSIGSDFGYFPKDLLEINHNYTNEELELPTEETDFVCFQDGRDDFDNYNVDELLKSSEETIANERDSESSDPGTKPAGTTEGDEEEGKVDTIEFHDTLESDNLELTTQEDKEALAVTEEVDSSLKEGTASAGINPTVNSHEEISQGEQIAHEHLQGMLNENLKGLESENTKNSSIPQGETSQFDKESEEVNAYTLLNRELSVNLKTKFGSTADAIVSDDEVTRLVTSMENDFNEDLSNNAYDAEEESDFVDQFEEIPLLSFMTEEESVSLVDSEADESNGFESKHHKLDEYANGATELHNQRDNEEERDSDVLITKDAFSENKNSGDSVNVDMFESEQTKEKRDEAMPVSKKEAPTATQLDDLSEELSRREPVGASDPDAKEKTNKTEQFEEQLLTDETVLHPEELKSTAVPDPRVLHSPGDGLKFKSFVEGKQDALSPPAGDVNQSPEGVLLAETEKEEEKFGGDTLKESSENNFKHRKLWEKMKEKGKAKYEPSGDVLAKPVEDVQNASQSDAGDTDLLKDKLEQEMPVVEKEILRQEEDLKQTREADHENQRPISVKKKPEIERGSMKETSADERDPSRRGAAEQPRPWENETEYSEADVKEELSRNLGKMTIFEERIEKSSPEEKSKSAAQNTNAENSTQQGTAHTDNEDSDRNLGTSSARETTLRLELQSEEPDAEEDPDLKQEEELLEDENAASAKLLQARLANIQGNAQTTRSTNPDLGVLSEAVSGTVNPTYETGEKTNSFSEDTKEISRVQDAHEVGNKEVDKPVSEDTKLDEIEHVIEDNKESSEAEEPSAVEEYDFKSPDREDSNDFNQRKGHLPESILLGDSEEIQNSEDTRNAHQHSAHFPSPADMSAATNDTVTDYSESVKRLTITRDFLDEKRVIRLQKYLGLQNVIRIEAMFQDMKIEMELARKASQNNEEIEKALDQILKSSDSSIMDVVGKVLDSRVAENQEEVVKEMDLYDEESALMDDIQELIYSLRSKYSSASESVPLGSFLEQEEDQLHVQGAAKEAENDAAPGRNLHGIDESSQKFQQLEDKRPFLPMEEEAEEEETAVNIPPEDEEATFSDNKEAEDGYGSERGLPLADPFGAADAGESAKGDTAPDHVPGVPPSLPPDPGAVPPGGGASEGALSGAVRRLLRQLAATLPEDIRPGPDFHGLPWEPVIVTALVGMATLAIIFWRTCLSVKSRMYQVTEKQLAEKIQNLLQEKTEMLDKFSECDEKIKQAKESMKVAQEQKSILSDEIAGLKDTVKELEETNHQLDDKIKSLRTMLDTERKQNAKKQKKLSETQKSLEKFEEAFSMHSAELSEVQIALNESKLSEEKVKAELQHVQEENARLKKSKEQLLKEAEGWSERHTELTEQIKLYRKSQKDIEEALAYKENEIEVLTNCIMQLKQLDTDSASEAKDGEGLEWSTKDDLANGELPDNEKMKTQIKQMMDASRVKTMLSLVEEDRNSLQSKLSDEVAARHELEEQIKKLEHDSSSLQSAKARLENECKTLQQKVEILGELYQQKEMALQKKLTQEEYERQEKEQKLSAADEKAVLAIEEVKVYKQRIQDMEEELQKTERSYKNQIAAHEKKAHDNWLIARSAERALAEEKREAANLRQKLMEVNQKTIMLQRPLIVKPTPGRPDRQVPPRRVPLSRDGSFGPSPVSGGNPSPTQMMEVPSRPLSAPQREGSRAEFGTVVDGPPAPRRPPELPGRMSVPDLGPAVASLISSGPRTSSPATAKDRAQPSPKEPEAPRVTTDSPSSIEPATVNVGPKGPPSFPGTPVMTSPVLGPPPPPPVNYGPPPAPFPGHYGPGPRPLPVPLVCGAPLPPPAARDFLPGPSLGIRDLPPGPLPPLPDPRSYRRGHPHFRPPGPPGPRDYPPGPPLPPPASRDYAPSRSRDLPPAGPRDYPAGPAPPPAGSKDYAQPPVQKP

Target ORF information:

RefSeq Version	XM_015283643.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283643.2

ORF Insert Sequence:

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCAGCCCT CTCCCAAAGA ACCTGAAGCC 
CCCCGTGTAA CTACCGATTC ACCTTCATCT ATTGAACCAG CTACAGTTAA CGTCGGTCCC 
AAAGGCCCAC CTTCTTTCCC TGGGACGCCC GTCATGACCT CTCCAGTGCT GGGACCTCCA 
CCTCCACCAC CTGTTAACTA TGGTCCACCA CCAGCTCCCT TTCCTGGACA CTACGGGCCT 
GGGCCTCGAC CTCTCCCTGT GCCCCTAGTT TGTGGTGCTC CCTTACCACC ACCAGCTGCA 
AGAGATTTCT TGCCTGGCCC ATCCTTAGGA ATAAGAGATC TGCCTCCTGG GCCACTGCCA 
CCCCTCCCAG ACCCCAGAAG CTACAGACGT GGGCACCCTC ATTTCCGGCC TCCAGGTCCT 
CCTGGCCCAC GTGATTATCC GCCAGGCCCG CCGCTACCAC CGCCTGCCTC CAGGGACTAC 
GCACCTTCTC GGAGCAGAGA CTTGCCTCCA GCAGGACCCA GAGACTACCC CGCAGGCCCC 
GCACCGCCCC CGGCAGGCTC AAAAGACTAC GCACAGCCTC CAGTGCAGAA GCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_015283644.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139130.2 Gallus gallus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

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CloneID	OGa148631
Clone ID Related Accession (Same CDS sequence)	XM_015283644.2
Accession Version	XM_015283644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transport and Golgi organization protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCCCTCTC CCAAAGAACC TGAAGCCCCC 
CGTGTAACTA CCGATTCACC TTCATCTATT GAACCAGCTA CAGTTAACGT CGGTCCCAAA 
GGCCCACCTT CTTTCCCTGG GACGCCCGTC ATGACCTCTC CAGTGCTGGG ACCTCCACCT 
CCACCACCTG TTAACTATGG TCCACCACCA GCTCCCTTTC CTGGACACTA CGGGCCTGGG 
CCTCGACCTC TCCCTGTGCC CCTAGTTTGT GGTGCTCCCT TACCACCACC AGCTGCAAGA 
GATTTCTTGC CTGGCCCATC CTTAGGAATA AGAGATCTGC CTCCTGGGCC ACTGCCACCC 
CTCCCAGACC CCAGAAGCTA CAGACGTGGG CACCCTCATT TCCGGCCTCC AGGTCCTCCT 
GGCCCACGTG ATTATCCGCC AGGCCCGCCG CTACCACCGC CTGCCTCCAG GGACTACGCA 
CCTTCTCGGA GCAGAGACTT GCCTCCAGCA GGACCCAGAG ACTACCCCGC AGGCCCCGCA 
CCGCCCCCGG CAGGCTCAAA AGACTACGCA CAGCCTCCAG TGCAGAAGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139130.2
CDS	45..6098
Translation	MAAAAPPDPRLLLAVLLLLPPPGPLCAAGPELSRRFTERKRCADPECSKLMCRGKAMKDFQGPDCRFVNFKKGETVYVYYKLIGMSTELWAGSIGSDFGYFPKDLLEINHNYTNEELELPTEETDFVCFQDGRDDFDNYNVDELLKSSEETIANERDSESSDPGTKPAGTTEGDEEEGKVDTIEFHDTLESDNLELTTQEDKEALAVTEEVDSSLKEGTASAGINPTVNSHEEISQGEQIAHEHLQGMLNENLKGLESENTKNSSIPQGETSQFDKESEEVNAYTLLNRELSVNLKTKFGSTADAIVSDDEVTRLVTSMENDFNEDLSNNAYDAEEESDFVDQFEEIPLLSFMTEEESVSLVDSEADESNGFESKHHKLDEYANGATELHNQRDNEEERDSDVLITKDAFSENKNSGDSVNVDMFESEQTKEKRDEAMPVSKKEAPTATQLDDLSEELSRREPVGASDPDAKEKTNKTEQFEEQLLTDETVLHPEELKSTAVPDPRVLHSPGDGLKFKSFVEGKQDALSPPAGDVNQSPEGVLLAETEKEEEKFGGDTLKESSENNFKHRKLWEKMKEKGKAKYEPSGDVLAKPVEDVQNASQSDAGDTDLLKDKLEQEMPVVEKEILRQEEDLKQTREADHENQRPISVKKKPEIERGSMKETSADERDPSRRGAAEQPRPWENETEYSEADVKEELSRNLGKMTIFEERIEKSSPEEKSKSAAQNTNAENSTQQGTAHTDNEDSDRNLGTSSARETTLRLELQSEEPDAEEDPDLKQEEELLEDENAASAKLLQARLANIQGNAQTTRSTNPDLGVLSEAVSGTVNPTYETGEKTNSFSEDTKEISRVQDAHEVGNKEVDKPVSEDTKLDEIEHVIEDNKESSEAEEPSAVEEYDFKSPDREDSNDFNQRKGHLPESILLGDSEEIQNSEDTRNAHQHSAHFPSPADMSAATNDTVTDYSESVKRLTITRDFLDEKRVIRLQKYLGLQNVIRIEAMFQDMKIEMELARKASQNNEEIEKALDQILKSSDSSIMDVVGKVLDSRVAENQEEVVKEMDLYDEESALMDDIQELIYSLRSKYSSASESVPLGSFLEQEEDQLHVQGAAKEAENDAAPGRNLHGIDESSQKFQQLEDKRPFLPMEEEAEEEETAVNIPPEDEEATFSDNKEAEDGYGSERGLPLADPFGAADAGESAKGDTAPDHVPGVPPSLPPDPGAVPPGGGASEGALSGAVRRLLRQLAATLPEDIRPGPDFHGLPWEPVIVTALVGMATLAIIFWRTCLSVKSRMYQVTEKQLAEKIQNLLQEKTEMLDKFSECDEKIKQAKESMKVAQEQKSILSDEIAGLKDTVKELEETNHQLDDKIKSLRTMLDTERKQNAKKQKKLSETQKSLEKFEEAFSMHSAELSEVQIALNESKLSEEKVKAELQHVQEENARLKKSKEQLLKEAEGWSERHTELTEQIKLYRKSQKDIEEALAYKENEIEVLTNCIMQLKQLDTDSASEAKDGEGLEWSTKDDLANGELPDNEKMKTQIKQMMDASRVKTMLSLVEEDRNSLQSKLSDEVAARHELEEQIKKLEHDSSSLQSAKARLENECKTLQQKVEILGELYQQKEMALQKKLTQEEYERQEKEQKLSAADEKAVLAIEEVKVYKQRIQDMEEELQKTERSYKNQIAAHEKKAHDNWLIARSAERALAEEKREAANLRQKLMEVNQKTIMLQRPLIVKPTPGRPDRQVPPRRVPLSRDGSFGPSPVSGGNPSPTQMMEVPSRPLSAPQREGSRAEFGTVVDGPPAPRRPPELPGRMSVPDLGPAVASLISSGPRTSSPATAKDRAPSPKEPEAPRVTTDSPSSIEPATVNVGPKGPPSFPGTPVMTSPVLGPPPPPPVNYGPPPAPFPGHYGPGPRPLPVPLVCGAPLPPPAARDFLPGPSLGIRDLPPGPLPPLPDPRSYRRGHPHFRPPGPPGPRDYPPGPPLPPPASRDYAPSRSRDLPPAGPRDYPAGPAPPPAGSKDYAQPPVQKP

Target ORF information:

RefSeq Version	XM_015283644.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283644.2

ORF Insert Sequence:

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCCCTCTC CCAAAGAACC TGAAGCCCCC 
CGTGTAACTA CCGATTCACC TTCATCTATT GAACCAGCTA CAGTTAACGT CGGTCCCAAA 
GGCCCACCTT CTTTCCCTGG GACGCCCGTC ATGACCTCTC CAGTGCTGGG ACCTCCACCT 
CCACCACCTG TTAACTATGG TCCACCACCA GCTCCCTTTC CTGGACACTA CGGGCCTGGG 
CCTCGACCTC TCCCTGTGCC CCTAGTTTGT GGTGCTCCCT TACCACCACC AGCTGCAAGA 
GATTTCTTGC CTGGCCCATC CTTAGGAATA AGAGATCTGC CTCCTGGGCC ACTGCCACCC 
CTCCCAGACC CCAGAAGCTA CAGACGTGGG CACCCTCATT TCCGGCCTCC AGGTCCTCCT 
GGCCCACGTG ATTATCCGCC AGGCCCGCCG CTACCACCGC CTGCCTCCAG GGACTACGCA 
CCTTCTCGGA GCAGAGACTT GCCTCCAGCA GGACCCAGAG ACTACCCCGC AGGCCCCGCA 
CCGCCCCCGG CAGGCTCAAA AGACTACGCA CAGCCTCCAG TGCAGAAGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001277558.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264487.1 Gallus gallus MIA family member 3, ER export factor (MIA3), mRNA.

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CloneID	OGa25229
Clone ID Related Accession (Same CDS sequence)	NM_001277558.2 , NM_001277558.3
Accession Version	NM_001277558.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	melanoma inhibitory activity protein 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000139.1, BU463685.1, BU218990.1, BU257487.1, AJ455758.1, CD734121.1, AI981292.1, BU345971.1, BU439596.1 and BG711556.1. On Feb 10, 2016 this sequence version replaced NM_001277558.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGGTTAACG TCGGTCCCAA AGGCCCACCT 
TCTTTCCCTG GGACGCCCGT CATGACCTCT CCAGTGCTGG GACCTCCACC TCCACCACCT 
GTTAACTATG GTCCACCACC AGCTCCCTTT CCTGGACACT ACGGGCCTGG GCCTCGACCT 
CTCCCTGTGC CCCTAGTTTG TGGTGCTCCC TTACCACCAC CAGCTGCAAG AGATTTCTTG 
CCTGGCCCAT CCTTAGGAAT AAGAGATCTG CCTCCTGGGC CACTGCCACC CCTCCCAGAC 
CCCAGAAGCT ACAGACGTGG GCACCCTCAT TTCCGGCCTC CAGGTCCTCC TGGCCCACGT 
GATTATCCGC CAGGCCCGCC GCTACCACCG CCTGCCTCCA GGGACTACGC ACCTTCTCGG 
AGCAGAGACT TGCCTCCAGC AGGACCCAGA GACTACCCCG CAGGCCCCGC ACCGCCCCCG 
GCAGGCTCAA AAGACTACGC ACAGCCTCCA GTGCAGAAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264487.1
CDS	1..5985
Translation	MAAAAPPDPRLLLAVLLLLPPPGPLCAAGPELSRRFTERKRCADPECSKLMCRGKAMKDFQGPDCRFVNFKKGETVYVYYKLIGMSTELWAGSIGSDFGYFPKDLLEINHNYTNEELELPTEETDFVCFQDGRDDFDNYNVDELLKSSEETIANERDSESSDPGTKPAGTTEGDEEEGKVDTIEFHDTLESDNLELTTQEDKEALAVTEEVDSSLKEGTASAGINPTVNSHEEISQGEQIAHEHLQGMLNENLKGLESENTKNSSIPQGETSQFDKESEEVNAYTLLNRELSVNLKTKFGSTADAIVSDDEVTRLVTSMENDFNEDLSNNAYDAEEESDFVDQFEEIPLLSFMTEEESVSLVDSEADESNGFESKHHKLDEYANGATELHNQRDNEEERDSDVLITKDAFSENKNSGDSVNVDMFESEQTKEKRDEAMPVSKKEAPTATQLDDLSEELSRREPVGASDPDAKEKTNKTEQFEEQLLTDETVLHPEELKSTAVPDPRVLHSPGDGLKFKSFVEGKQDALSPPAGDVNQSPEGVLLAETEKEEEKFGGDTLKESSENNFKHRKLWEKMKEKGKAKYEPSGDVLAKPVEDVQNASQSDAGDTDLLKDKLEQEMPVVEKEILRQEEDLKQTREADHENQRPISVKKKPEIERGSMKETSADERDPSRRGAAEQPRPWENETEYSEADVKEELSRNLGKMTIFEERIEKSSPEEKSKSAAQNTNAENSTQQGTAHTDNEDSDRNLGTSSARETTLRLELQSEEPDAEEDPDLKQEEELLEDENAASAKLLQARLANIQGNAQTTRSTNPDLGVLSEAVSGTVNPTYETGEKTNSFSEDTKEISRVQDAHEVGNKEVDKPVSEDTKLDEIEHVIEDNKESSEAEEPSAVEEYDFKSPDREDSNDFNQRKGHLPESILLGDSEEIQNSEDTRNAHQHSAHFPSPADMSAATNDTVTDYSESVKRLTITRDFLDEKRVIRLQKYLGLQNVIRIEAMFQDMKIEMELARKASQNNEEIEKALDQILKSSDSSIMDVVGKVLDSRVAENQEEVVKEMDLYDEESALMDDIQELIYSLRSKYSSASESVPLGSFLEQEEDQLHVQGAAKEAENDAAPGRNLHGIDESSQKFQQLEDKRPFLPMEEEAEEEETAVNIPPEDEEATFSDNKEAEDGYGSERGLPLADPFGAADAGESAKGDTAPDHVPGVPPSLPPDPGAVPPGGGASEGALSGAVRRLLRQLAATLPEDIRPGPDFHGLPWEPVIVTALVGMATLAIIFWRTCLSVKSRMYQVTEKQLAEKIQNLLQEKTEMLDKFSECDEKIKQAKESMKVAQEQKSILSDEIAGLKDTVKELEETNHQLDDKIKSLRTMLDTERKQNAKKQKKLSETQKSLEKFEEAFSMHSAELSEVQIALNESKLSEEKVKAELQHVQEENARLKKSKEQLLKEAEGWSERHTELTEQIKLYRKSQKDIEEALAYKENEIEVLTNCIMQLKQLDTDSASEAKDGEGLEWSTKDDLANGELPDNEKMKTQIKQMMDASRVKTMLSLVEEDRNSLQSKLSDEVAARHELEEQIKKLEHDSSSLQSAKARLENECKTLQQKVEILGELYQQKEMALQKKLTQEEYERQEKEQKLSAADEKAVLAIEEVKVYKQRIQDMEEELQKTERSYKNQIAAHEKKAHDNWLIARSAERALAEEKREAANLRQKLMEVNQKTIMLQRPLIVKPTPGRPDRQVPPRRVPLSRDGSFGPSPVSGGNPSPTQMMEVPSRPLSAPQREGSRAEFGTVVDGPPAPRRPPELPGRMSVPDLGPAVASLISSGPRTSSPATAKDRAVNVGPKGPPSFPGTPVMTSPVLGPPPPPPVNYGPPPAPFPGHYGPGPRPLPVPLVCGAPLPPPAARDFLPGPSLGIRDLPPGPLPPLPDPRSYRRGHPHFRPPGPPGPRDYPPGPPLPPPASRDYAPSRSRDLPPAGPRDYPAGPAPPPAGSKDYAQPPVQKP

Target ORF information:

RefSeq Version	NM_001277558.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MIA family member 3, ER export factor (MIA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277558.2

ORF Insert Sequence:

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGGTTAACG TCGGTCCCAA AGGCCCACCT 
TCTTTCCCTG GGACGCCCGT CATGACCTCT CCAGTGCTGG GACCTCCACC TCCACCACCT 
GTTAACTATG GTCCACCACC AGCTCCCTTT CCTGGACACT ACGGGCCTGG GCCTCGACCT 
CTCCCTGTGC CCCTAGTTTG TGGTGCTCCC TTACCACCAC CAGCTGCAAG AGATTTCTTG 
CCTGGCCCAT CCTTAGGAAT AAGAGATCTG CCTCCTGGGC CACTGCCACC CCTCCCAGAC 
CCCAGAAGCT ACAGACGTGG GCACCCTCAT TTCCGGCCTC CAGGTCCTCC TGGCCCACGT 
GATTATCCGC CAGGCCCGCC GCTACCACCG CCTGCCTCCA GGGACTACGC ACCTTCTCGG 
AGCAGAGACT TGCCTCCAGC AGGACCCAGA GACTACCCCG CAGGCCCCGC ACCGCCCCCG 
GCAGGCTCAA AAGACTACGC ACAGCCTCCA GTGCAGAAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001277558.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001264487.1 Gallus gallus MIA family member 3, ER export factor (MIA3), mRNA.

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CloneID	OGa25229
Clone ID Related Accession (Same CDS sequence)	NM_001277558.2 , NM_001277558.3
Accession Version	NM_001277558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-25
Organism	Gallus gallus(chicken)
Product	transport and Golgi organization protein 1 homolog precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000005.1, BU463685.1, BU218990.1, BU257487.1, AJ455758.1, CD734121.1, AI981292.1, BU345971.1, BU439596.1 and BG711556.1. On Jun 26, 2018 this sequence version replaced NM_001277558.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGGTTAACG TCGGTCCCAA AGGCCCACCT 
TCTTTCCCTG GGACGCCCGT CATGACCTCT CCAGTGCTGG GACCTCCACC TCCACCACCT 
GTTAACTATG GTCCACCACC AGCTCCCTTT CCTGGACACT ACGGGCCTGG GCCTCGACCT 
CTCCCTGTGC CCCTAGTTTG TGGTGCTCCC TTACCACCAC CAGCTGCAAG AGATTTCTTG 
CCTGGCCCAT CCTTAGGAAT AAGAGATCTG CCTCCTGGGC CACTGCCACC CCTCCCAGAC 
CCCAGAAGCT ACAGACGTGG GCACCCTCAT TTCCGGCCTC CAGGTCCTCC TGGCCCACGT 
GATTATCCGC CAGGCCCGCC GCTACCACCG CCTGCCTCCA GGGACTACGC ACCTTCTCGG 
AGCAGAGACT TGCCTCCAGC AGGACCCAGA GACTACCCCG CAGGCCCCGC ACCGCCCCCG 
GCAGGCTCAA AAGACTACGC ACAGCCTCCA GTGCAGAAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264487.1
CDS	1..5985
Translation	MAAAAPPDPRLLLAVLLLLPPPGPLCAAGPELSRRFTERKRCADPECSKLMCRGKAMKDFQGPDCRFVNFKKGETVYVYYKLIGMSTELWAGSIGSDFGYFPKDLLEINHNYTNEELELPTEETDFVCFQDGRDDFDNYNVDELLKSSEETIANERDSESSDPGTKPAGTTEGDEEEGKVDTIEFHDTLESDNLELTTQEDKEALAVTEEVDSSLKEGTASAGINPTVNSHEEISQGEQIAHEHLQGMLNENLKGLESENTKNSSIPQGETSQFDKESEEVNAYTLLNRELSVNLKTKFGSTADAIVSDDEVTRLVTSMENDFNEDLSNNAYDAEEESDFVDQFEEIPLLSFMTEEESVSLVDSEADESNGFESKHHKLDEYANGATELHNQRDNEEERDSDVLITKDAFSENKNSGDSVNVDMFESEQTKEKRDEAMPVSKKEAPTATQLDDLSEELSRREPVGASDPDAKEKTNKTEQFEEQLLTDETVLHPEELKSTAVPDPRVLHSPGDGLKFKSFVEGKQDALSPPAGDVNQSPEGVLLAETEKEEEKFGGDTLKESSENNFKHRKLWEKMKEKGKAKYEPSGDVLAKPVEDVQNASQSDAGDTDLLKDKLEQEMPVVEKEILRQEEDLKQTREADHENQRPISVKKKPEIERGSMKETSADERDPSRRGAAEQPRPWENETEYSEADVKEELSRNLGKMTIFEERIEKSSPEEKSKSAAQNTNAENSTQQGTAHTDNEDSDRNLGTSSARETTLRLELQSEEPDAEEDPDLKQEEELLEDENAASAKLLQARLANIQGNAQTTRSTNPDLGVLSEAVSGTVNPTYETGEKTNSFSEDTKEISRVQDAHEVGNKEVDKPVSEDTKLDEIEHVIEDNKESSEAEEPSAVEEYDFKSPDREDSNDFNQRKGHLPESILLGDSEEIQNSEDTRNAHQHSAHFPSPADMSAATNDTVTDYSESVKRLTITRDFLDEKRVIRLQKYLGLQNVIRIEAMFQDMKIEMELARKASQNNEEIEKALDQILKSSDSSIMDVVGKVLDSRVAENQEEVVKEMDLYDEESALMDDIQELIYSLRSKYSSASESVPLGSFLEQEEDQLHVQGAAKEAENDAAPGRNLHGIDESSQKFQQLEDKRPFLPMEEEAEEEETAVNIPPEDEEATFSDNKEAEDGYGSERGLPLADPFGAADAGESAKGDTAPDHVPGVPPSLPPDPGAVPPGGGASEGALSGAVRRLLRQLAATLPEDIRPGPDFHGLPWEPVIVTALVGMATLAIIFWRTCLSVKSRMYQVTEKQLAEKIQNLLQEKTEMLDKFSECDEKIKQAKESMKVAQEQKSILSDEIAGLKDTVKELEETNHQLDDKIKSLRTMLDTERKQNAKKQKKLSETQKSLEKFEEAFSMHSAELSEVQIALNESKLSEEKVKAELQHVQEENARLKKSKEQLLKEAEGWSERHTELTEQIKLYRKSQKDIEEALAYKENEIEVLTNCIMQLKQLDTDSASEAKDGEGLEWSTKDDLANGELPDNEKMKTQIKQMMDASRVKTMLSLVEEDRNSLQSKLSDEVAARHELEEQIKKLEHDSSSLQSAKARLENECKTLQQKVEILGELYQQKEMALQKKLTQEEYERQEKEQKLSAADEKAVLAIEEVKVYKQRIQDMEEELQKTERSYKNQIAAHEKKAHDNWLIARSAERALAEEKREAANLRQKLMEVNQKTIMLQRPLIVKPTPGRPDRQVPPRRVPLSRDGSFGPSPVSGGNPSPTQMMEVPSRPLSAPQREGSRAEFGTVVDGPPAPRRPPELPGRMSVPDLGPAVASLISSGPRTSSPATAKDRAVNVGPKGPPSFPGTPVMTSPVLGPPPPPPVNYGPPPAPFPGHYGPGPRPLPVPLVCGAPLPPPAARDFLPGPSLGIRDLPPGPLPPLPDPRSYRRGHPHFRPPGPPGPRDYPPGPPLPPPASRDYAPSRSRDLPPAGPRDYPAGPAPPPAGSKDYAQPPVQKP

Target ORF information:

RefSeq Version	NM_001277558.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus MIA family member 3, ER export factor (MIA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277558.3

ORF Insert Sequence:

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGGTTAACG TCGGTCCCAA AGGCCCACCT 
TCTTTCCCTG GGACGCCCGT CATGACCTCT CCAGTGCTGG GACCTCCACC TCCACCACCT 
GTTAACTATG GTCCACCACC AGCTCCCTTT CCTGGACACT ACGGGCCTGG GCCTCGACCT 
CTCCCTGTGC CCCTAGTTTG TGGTGCTCCC TTACCACCAC CAGCTGCAAG AGATTTCTTG 
CCTGGCCCAT CCTTAGGAAT AAGAGATCTG CCTCCTGGGC CACTGCCACC CCTCCCAGAC 
CCCAGAAGCT ACAGACGTGG GCACCCTCAT TTCCGGCCTC CAGGTCCTCC TGGCCCACGT 
GATTATCCGC CAGGCCCGCC GCTACCACCG CCTGCCTCCA GGGACTACGC ACCTTCTCGG 
AGCAGAGACT TGCCTCCAGC AGGACCCAGA GACTACCCCG CAGGCCCCGC ACCGCCCCCG 
GCAGGCTCAA AAGACTACGC ACAGCCTCCA GTGCAGAAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_015283643.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139129.1 Gallus gallus melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.

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CloneID	OGa33332
Clone ID Related Accession (Same CDS sequence)	XM_015283643.1
Accession Version	XM_015283643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455833.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCAGCCCT CTCCCAAAGA ACCTGAAGCC 
CCCCGTGTAA CTACCGATTC ACCTTCATCT ATTGAACCAG CTACAGTTAA CGTCGGTCCC 
AAAGGCCCAC CTTCTTTCCC TGGGACGCCC GTCATGACCT CTCCAGTGCT GGGACCTCCA 
CCTCCACCAC CTGTTAACTA TGGTCCACCA CCAGCTCCCT TTCCTGGACA CTACGGGCCT 
GGGCCTCGAC CTCTCCCTGT GCCCCTAGTT TGTGGTGCTC CCTTACCACC ACCAGCTGCA 
AGAGATTTCT TGCCTGGCCC ATCCTTAGGA ATAAGAGATC TGCCTCCTGG GCCACTGCCA 
CCCCTCCCAG ACCCCAGAAG CTACAGACGT GGGCCCCCTC ATTTCCAGCC TCTAGGTCCT 
CCTAGCCCAC ATGATTATCT GCCAGGCCCG CCGCTACCAC CGCCTGCCTC CAGGGACTAC 
GCACCTTCTC GGAGCAGAGA CTTGCCTCCA GCAGGACCCA GAGACTACCC CGCAGGCCCC 
GCACCGCCCC CGGCAGGCTC AAAAGACTAC GCACAGCCTC CAGTGCAGAA GCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139129.1
CDS	45..6101
Translation	MAAAAPPDPRLLLAVLLLLPPPGPLCAAGPELSRRFTERKRCADPECSKLMCRGKAMKDFQGPDCRFVNFKKGETVYVYYKLIGMSTELWAGSIGSDFGYFPKDLLEINHNYTNEELELPTEETDFVCFQDGRDDFDNYNVDELLKSSEETIANERDSESSDPGTKPAGTTEGDEEEGKVDTIEFHDTLESDNLELTTQEDKEALAVTEEVDSSLKEGTASAGINPTVNSHEEISQGEQIAHEHLQGMLNENLKGLESENTKNSSIPQGETSQFDKESEEVNAYTLLNRELSVNLKTKFGSTADAIVSDDEVTRLVTSMENDFNEDLSNNAYDAEEESDFVDQFEEIPLLSFMTEEESVSLVDSEADESNGFESKHHKLDEYANGATELHNQRDNEEERDSDVLITKDAFSENKNSGDSVNVDMFESEQTKEKRDEAMPVSKKEAPTATQLDDLSEELSRREPVGASDPDAKEKTNKTEQFEEQLLTDETVLHPEELKSTAVPDPRVLHSPGDGLKFKSFVEGKQDALSPPAGDVNQSPEGVLLAETEKEEEKFGGDTLKESSENNFKHRKLWEKMKEKGKAKYEPSGDVLAKPVEDVQNASQSDAGDTDLLKDKLEQEMPVVEKEILRQEEDLKQTREADHENQRPISVKKKPEIERGSMKETSADERDPSRRGAAEQPRPWENETEYSEADVKEELSRNLGKMTIFEERIEKSSPEEKSKSAAQNTNAENSTQQGTAHTDNEDSDRNLGTSSARETTLRLELQSEEPDAEEDPDLKQEEELLEDENAASAKLLQARLANIQGNAQTTRSTNPDLGVLSEAVSGTVNPTYETGEKTNSFSEDTKEISRVQDAHEVGNKEVDKPVSEDTKLDEIEHVIEDNKESSEAEEPSAVEEYDFKSPDREDSNDFNQRKGHLPESILLGDSEEIQNSEDTRNAHQHSAHFPSPADMSAATNDTVTDYSESVKRLTITRDFLDEKRVIRLQKYLGLQNVIRIEAMFQDMKIEMELARKASQNNEEIEKALDQILKSSDSSIMDVVGKVLDSRVAENQEEVVKEMDLYDEESALMDDIQELIYSLRSKYSSASESVPLGSFLEQEEDQLHVQGAAKEAENDAAPGRNLHGIDESSQKFQQLEDKRPFLPMEEEAEEEETAVNIPPEDEEATFSDNKEAEDGYGSERGLPLADPFGAADAGESAKGDTAPDHVPGVPPSLPPDPGAVPPGGGASEGALSGAVRRLLRQLAATLPEDIRPGPDFHGLPWEPVIVTALVGMATLAIIFWRTCLSVKSRMYQVTEKQLAEKIQNLLQEKTEMLDKFSECDEKIKQAKESMKVAQEQKSILSDEIAGLKDTVKELEETNHQLDDKIKSLRTMLDTERKQNAKKQKKLSETQKSLEKFEEAFSMHSAELSEVQIALNESKLSEEKVKAELQHVQEENARLKKSKEQLLKEAEGWSERHTELTEQIKLYRKSQKDIEEALAYKENEIEVLTNCIMQLKQLDTDSASEAKDGEGLEWSTKDDLANGELPDNEKMKTQIKQMMDASRVKTMLSLVEEDRNSLQSKLSDEVAARHELEEQIKKLEHDSSSLQSAKARLENECKTLQQKVEILGELYQQKEMALQKKLTQEEYERQEKEQKLSAADEKAVLAIEEVKVYKQRIQDMEEELQKTERSYKNQIAAHEKKAHDNWLIARSAERALAEEKREAANLRQKLMEVNQKTIMLQRPLIVKPTPGRPDRQVPPRRVPLSRDGSFGPSPVSGGNPSPTQMMEVPSRPLSAPQREGSRAEFGTVVDGPPAPRRPPELPGRMSVPDLGPAVASLISSGPRTSSPATAKDRAQPSPKEPEAPRVTTDSPSSIEPATVNVGPKGPPSFPGTPVMTSPVLGPPPPPPVNYGPPPAPFPGHYGPGPRPLPVPLVCGAPLPPPAARDFLPGPSLGIRDLPPGPLPPLPDPRSYRRGPPHFQPLGPPSPHDYLPGPPLPPPASRDYAPSRSRDLPPAGPRDYPAGPAPPPAGSKDYAQPPVQKP

Target ORF information:

RefSeq Version	XM_015283643.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283643.1

ORF Insert Sequence:

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCAGCCCT CTCCCAAAGA ACCTGAAGCC 
CCCCGTGTAA CTACCGATTC ACCTTCATCT ATTGAACCAG CTACAGTTAA CGTCGGTCCC 
AAAGGCCCAC CTTCTTTCCC TGGGACGCCC GTCATGACCT CTCCAGTGCT GGGACCTCCA 
CCTCCACCAC CTGTTAACTA TGGTCCACCA CCAGCTCCCT TTCCTGGACA CTACGGGCCT 
GGGCCTCGAC CTCTCCCTGT GCCCCTAGTT TGTGGTGCTC CCTTACCACC ACCAGCTGCA 
AGAGATTTCT TGCCTGGCCC ATCCTTAGGA ATAAGAGATC TGCCTCCTGG GCCACTGCCA 
CCCCTCCCAG ACCCCAGAAG CTACAGACGT GGGCCCCCTC ATTTCCAGCC TCTAGGTCCT 
CCTAGCCCAC ATGATTATCT GCCAGGCCCG CCGCTACCAC CGCCTGCCTC CAGGGACTAC 
GCACCTTCTC GGAGCAGAGA CTTGCCTCCA GCAGGACCCA GAGACTACCC CGCAGGCCCC 
GCACCGCCCC CGGCAGGCTC AAAAGACTAC GCACAGCCTC CAGTGCAGAA GCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_015283644.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139130.1 Gallus gallus melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.

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CloneID	OGa33333
Clone ID Related Accession (Same CDS sequence)	XM_015283644.1
Accession Version	XM_015283644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	melanoma inhibitory activity protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455833.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCCCTCTC CCAAAGAACC TGAAGCCCCC 
CGTGTAACTA CCGATTCACC TTCATCTATT GAACCAGCTA CAGTTAACGT CGGTCCCAAA 
GGCCCACCTT CTTTCCCTGG GACGCCCGTC ATGACCTCTC CAGTGCTGGG ACCTCCACCT 
CCACCACCTG TTAACTATGG TCCACCACCA GCTCCCTTTC CTGGACACTA CGGGCCTGGG 
CCTCGACCTC TCCCTGTGCC CCTAGTTTGT GGTGCTCCCT TACCACCACC AGCTGCAAGA 
GATTTCTTGC CTGGCCCATC CTTAGGAATA AGAGATCTGC CTCCTGGGCC ACTGCCACCC 
CTCCCAGACC CCAGAAGCTA CAGACGTGGG CCCCCTCATT TCCAGCCTCT AGGTCCTCCT 
AGCCCACATG ATTATCTGCC AGGCCCGCCG CTACCACCGC CTGCCTCCAG GGACTACGCA 
CCTTCTCGGA GCAGAGACTT GCCTCCAGCA GGACCCAGAG ACTACCCCGC AGGCCCCGCA 
CCGCCCCCGG CAGGCTCAAA AGACTACGCA CAGCCTCCAG TGCAGAAGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139130.1
CDS	45..6098
Translation	MAAAAPPDPRLLLAVLLLLPPPGPLCAAGPELSRRFTERKRCADPECSKLMCRGKAMKDFQGPDCRFVNFKKGETVYVYYKLIGMSTELWAGSIGSDFGYFPKDLLEINHNYTNEELELPTEETDFVCFQDGRDDFDNYNVDELLKSSEETIANERDSESSDPGTKPAGTTEGDEEEGKVDTIEFHDTLESDNLELTTQEDKEALAVTEEVDSSLKEGTASAGINPTVNSHEEISQGEQIAHEHLQGMLNENLKGLESENTKNSSIPQGETSQFDKESEEVNAYTLLNRELSVNLKTKFGSTADAIVSDDEVTRLVTSMENDFNEDLSNNAYDAEEESDFVDQFEEIPLLSFMTEEESVSLVDSEADESNGFESKHHKLDEYANGATELHNQRDNEEERDSDVLITKDAFSENKNSGDSVNVDMFESEQTKEKRDEAMPVSKKEAPTATQLDDLSEELSRREPVGASDPDAKEKTNKTEQFEEQLLTDETVLHPEELKSTAVPDPRVLHSPGDGLKFKSFVEGKQDALSPPAGDVNQSPEGVLLAETEKEEEKFGGDTLKESSENNFKHRKLWEKMKEKGKAKYEPSGDVLAKPVEDVQNASQSDAGDTDLLKDKLEQEMPVVEKEILRQEEDLKQTREADHENQRPISVKKKPEIERGSMKETSADERDPSRRGAAEQPRPWENETEYSEADVKEELSRNLGKMTIFEERIEKSSPEEKSKSAAQNTNAENSTQQGTAHTDNEDSDRNLGTSSARETTLRLELQSEEPDAEEDPDLKQEEELLEDENAASAKLLQARLANIQGNAQTTRSTNPDLGVLSEAVSGTVNPTYETGEKTNSFSEDTKEISRVQDAHEVGNKEVDKPVSEDTKLDEIEHVIEDNKESSEAEEPSAVEEYDFKSPDREDSNDFNQRKGHLPESILLGDSEEIQNSEDTRNAHQHSAHFPSPADMSAATNDTVTDYSESVKRLTITRDFLDEKRVIRLQKYLGLQNVIRIEAMFQDMKIEMELARKASQNNEEIEKALDQILKSSDSSIMDVVGKVLDSRVAENQEEVVKEMDLYDEESALMDDIQELIYSLRSKYSSASESVPLGSFLEQEEDQLHVQGAAKEAENDAAPGRNLHGIDESSQKFQQLEDKRPFLPMEEEAEEEETAVNIPPEDEEATFSDNKEAEDGYGSERGLPLADPFGAADAGESAKGDTAPDHVPGVPPSLPPDPGAVPPGGGASEGALSGAVRRLLRQLAATLPEDIRPGPDFHGLPWEPVIVTALVGMATLAIIFWRTCLSVKSRMYQVTEKQLAEKIQNLLQEKTEMLDKFSECDEKIKQAKESMKVAQEQKSILSDEIAGLKDTVKELEETNHQLDDKIKSLRTMLDTERKQNAKKQKKLSETQKSLEKFEEAFSMHSAELSEVQIALNESKLSEEKVKAELQHVQEENARLKKSKEQLLKEAEGWSERHTELTEQIKLYRKSQKDIEEALAYKENEIEVLTNCIMQLKQLDTDSASEAKDGEGLEWSTKDDLANGELPDNEKMKTQIKQMMDASRVKTMLSLVEEDRNSLQSKLSDEVAARHELEEQIKKLEHDSSSLQSAKARLENECKTLQQKVEILGELYQQKEMALQKKLTQEEYERQEKEQKLSAADEKAVLAIEEVKVYKQRIQDMEEELQKTERSYKNQIAAHEKKAHDNWLIARSAERALAEEKREAANLRQKLMEVNQKTIMLQRPLIVKPTPGRPDRQVPPRRVPLSRDGSFGPSPVSGGNPSPTQMMEVPSRPLSAPQREGSRAEFGTVVDGPPAPRRPPELPGRMSVPDLGPAVASLISSGPRTSSPATAKDRAPSPKEPEAPRVTTDSPSSIEPATVNVGPKGPPSFPGTPVMTSPVLGPPPPPPVNYGPPPAPFPGHYGPGPRPLPVPLVCGAPLPPPAARDFLPGPSLGIRDLPPGPLPPLPDPRSYRRGPPHFQPLGPPSPHDYLPGPPLPPPASRDYAPSRSRDLPPAGPRDYPAGPAPPPAGSKDYAQPPVQKP

Target ORF information:

RefSeq Version	XM_015283644.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283644.1

ORF Insert Sequence:

ATGGCCGCAG CGGCGCCGCC GGACCCTCGG CTGCTCCTGG CCGTGCTGCT GCTCCTGCCG 
CCGCCGGGCC CTCTCTGCGC CGCCGGGCCC GAGCTGAGCC GCCGCTTCAC CGAGCGCAAG 
CGCTGCGCCG ACCCCGAGTG CAGCAAACTA ATGTGCCGAG GGAAAGCAAT GAAGGATTTT 
CAAGGTCCGG ACTGTCGCTT TGTAAATTTT AAGAAAGGAG AAACAGTATA CGTGTATTAC 
AAACTAATAG GAATGTCAAC TGAGCTTTGG GCTGGAAGTA TTGGAAGTGA TTTTGGATAT 
TTTCCAAAGG ATTTACTTGA AATAAATCAT AATTATACCA ATGAGGAGCT AGAGTTACCA 
ACAGAAGAAA CAGACTTTGT TTGCTTTCAA GATGGAAGAG ATGATTTTGA TAACTATAAC 
GTGGATGAAC TTCTGAAGTC ATCGGAAGAG ACGATAGCAA ATGAAAGGGA CTCTGAATCA 
AGTGATCCAG GGACAAAACC AGCTGGAACA ACTGAAGGAG ATGAAGAGGA GGGAAAGGTT 
GATACGATAG AGTTCCATGA TACTTTGGAG TCAGACAATC TTGAACTAAC TACACAGGAA 
GATAAGGAAG CCCTTGCTGT GACAGAGGAG GTAGACAGTT CTCTTAAAGA AGGAACTGCA 
AGTGCTGGGA TAAACCCCAC TGTTAATAGT CATGAAGAAA TCTCTCAGGG AGAACAAATT 
GCACATGAGC ACTTACAAGG AATGTTAAAT GAGAATCTAA AAGGGCTAGA AAGTGAAAAT 
ACCAAAAACT CTAGTATTCC TCAGGGTGAA ACTAGTCAAT TTGACAAAGA GAGTGAAGAA 
GTCAATGCCT ATACACTTTT AAACAGAGAG CTCTCTGTGA ACTTGAAAAC AAAATTTGGC 
TCAACTGCTG ATGCTATTGT ATCAGATGAT GAAGTGACTC GCCTCGTTAC ATCGATGGAA 
AATGATTTTA ATGAAGATTT GAGCAATAAT GCTTATGATG CAGAGGAGGA GTCAGACTTT 
GTAGATCAGT TTGAAGAAAT TCCTTTGCTG TCATTTATGA CAGAAGAAGA AAGCGTATCC 
CTAGTGGATT CAGAAGCTGA TGAAAGCAAT GGCTTTGAGT CAAAACATCA TAAACTTGAT 
GAATATGCAA ATGGTGCTAC AGAGCTACAC AACCAAAGAG ACAATGAGGA AGAACGTGAT 
TCAGATGTGT TAATTACTAA GGATGCCTTC AGTGAGAACA AGAACTCGGG TGATAGTGTA 
AATGTGGACA TGTTTGAATC TGAACAAACA AAGGAGAAGC GTGATGAAGC AATGCCAGTT 
AGCAAGAAAG AAGCACCAAC AGCAACTCAA CTTGATGATC TCTCCGAAGA GCTCAGTAGA 
AGAGAACCTG TAGGTGCAAG TGACCCAGAT GCAAAAGAAA AAACAAACAA AACTGAACAG 
TTTGAAGAGC AGCTCCTGAC TGATGAAACA GTGTTGCATC CTGAAGAGCT GAAGAGTACA 
GCTGTGCCTG ATCCCCGTGT TTTGCATAGT CCAGGAGATG GTCTGAAATT CAAATCATTT 
GTTGAAGGTA AGCAAGATGC TCTAAGTCCA CCTGCTGGTG ATGTGAACCA AAGTCCAGAA 
GGAGTGCTAC TTGCTGAAAC GGAGAAAGAA GAGGAAAAGT TTGGAGGTGA CACCTTGAAG 
GAGAGCTCAG AAAATAATTT TAAGCACAGA AAGTTATGGG AGAAAATGAA GGAGAAAGGA 
AAAGCTAAGT ATGAGCCTTC TGGTGATGTT CTAGCAAAAC CAGTGGAAGA TGTACAAAAT 
GCATCTCAAA GTGATGCAGG AGATACTGAC CTTCTGAAAG ACAAACTGGA GCAAGAAATG 
CCTGTGGTGG AAAAAGAAAT CCTAAGACAG GAAGAAGATT TGAAACAAAC AAGGGAGGCT 
GATCATGAAA ATCAAAGGCC CATCTCTGTT AAGAAAAAAC CAGAAATAGA GAGGGGAAGC 
ATGAAAGAAA CCTCTGCAGA TGAGAGAGAC CCAAGCCGTA GAGGAGCTGC TGAACAACCT 
AGGCCATGGG AAAATGAGAC TGAGTATTCA GAGGCAGATG TGAAAGAAGA GCTTTCAAGA 
AATCTTGGCA AGATGACAAT ATTTGAAGAA AGAATCGAGA AAAGTTCCCC TGAAGAAAAA 
TCAAAAAGTG CTGCACAGAA TACTAATGCA GAGAATTCTA CTCAGCAAGG AACTGCTCAT 
ACTGATAATG AAGACTCAGA CAGAAATCTT GGCACAAGTT CAGCTAGAGA AACAACCCTA 
AGGCTAGAAT TGCAGTCTGA AGAGCCAGAT GCTGAAGAAG ACCCTGACTT GAAGCAAGAG 
GAAGAACTAC TGGAAGATGA AAATGCTGCA AGTGCAAAGC TGTTGCAAGC AAGGCTTGCA 
AATATACAGG GAAATGCTCA GACAACTAGA AGTACAAATC CTGATTTAGG AGTACTGAGT 
GAAGCTGTTT CAGGAACTGT AAATCCTACT TATGAAACGG GAGAGAAAAC AAACTCATTT 
TCTGAGGACA CTAAAGAAAT AAGCAGGGTG CAGGATGCGC ATGAGGTCGG AAACAAAGAG 
GTTGACAAAC CAGTGAGTGA AGATACCAAA TTAGATGAGA TTGAACATGT CATAGAAGAC 
AACAAAGAGT CTTCTGAAGC TGAGGAACCA TCAGCTGTGG AAGAATATGA TTTCAAATCT 
CCTGACAGAG AGGACAGCAA TGACTTTAAC CAAAGGAAAG GCCATCTCCC AGAGAGCATT 
TTGCTGGGAG ATTCAGAAGA GATTCAAAAT TCAGAGGATA CAAGAAACGC CCACCAGCAT 
TCTGCACATT TCCCCAGCCC TGCTGACATG TCAGCAGCCA CAAATGACAC TGTAACAGAC 
TACAGTGAGT CTGTGAAGCG ACTCACAATA ACAAGAGATT TCCTTGATGA AAAGCGTGTG 
ATACGCCTAC AGAAGTACCT TGGGCTCCAA AATGTGATTA GGATTGAAGC CATGTTTCAG 
GACATGAAGA TAGAGATGGA GCTTGCTCGG AAGGCAAGCC AGAACAATGA AGAAATAGAA 
AAAGCTTTGG ACCAGATACT TAAGTCTTCA GACTCGAGCA TTATGGATGT TGTAGGAAAG 
GTCCTGGATT CCAGAGTGGC AGAAAATCAA GAGGAGGTGG TGAAAGAAAT GGATTTATAT 
GATGAAGAAA GCGCACTGAT GGACGATATT CAAGAATTAA TATACTCTTT AAGAAGTAAA 
TACTCATCTG CTAGTGAGAG TGTCCCACTT GGTTCTTTTC TAGAGCAGGA AGAAGATCAG 
CTGCATGTTC AAGGTGCTGC AAAAGAAGCT GAAAATGATG CAGCTCCTGG CAGAAACCTA 
CATGGCATTG ATGAGAGCAG TCAGAAGTTT CAGCAGCTTG AAGACAAGAG GCCATTTCTT 
CCTATGGAGG AGGAGGCAGA GGAGGAGGAG ACAGCTGTTA ATATTCCACC TGAAGATGAG 
GAGGCCACCT TCTCAGATAA CAAAGAAGCC GAAGATGGGT ACGGCAGTGA AAGAGGATTG 
CCGCTGGCTG ATCCCTTTGG GGCAGCTGAT GCAGGAGAGA GTGCTAAGGG AGACACTGCT 
CCAGACCACG TTCCGGGTGT CCCTCCCTCC CTCCCTCCGG ACCCCGGCGC CGTGCCGCCG 
GGAGGCGGCG CCTCAGAGGG AGCGCTGAGC GGGGCGGTGC GCCGTTTGCT GCGCCAGTTG 
GCTGCCACCC TGCCTGAGGA TATTCGTCCT GGGCCTGATT TCCATGGCCT GCCGTGGGAG 
CCCGTCATTG TCACTGCCTT AGTGGGGATG GCCACGCTTG CTATCATTTT CTGGAGAACC 
TGCCTTTCGG TAAAGAGTAG AATGTATCAA GTGACTGAAA AGCAATTGGC TGAGAAGATT 
CAAAACCTCC TACAAGAAAA AACAGAAATG TTAGACAAGT TTTCAGAGTG CGATGAAAAG 
ATAAAGCAAG CAAAGGAATC GATGAAAGTG GCCCAAGAAC AAAAAAGCAT TCTCTCAGAT 
GAAATTGCAG GGCTTAAGGA CACTGTCAAA GAACTAGAAG AAACAAATCA TCAGCTGGAT 
GACAAAATAA AAAGCCTGCG CACAATGCTT GACACAGAGA GAAAACAGAA TGCCAAGAAA 
CAGAAAAAAC TCTCTGAAAC CCAGAAGTCA TTGGAGAAAT TTGAAGAGGC CTTCAGCATG 
CATTCTGCAG AGCTGTCAGA GGTGCAAATA GCCCTTAATG AATCTAAACT AAGTGAAGAA 
AAGGTGAAAG CAGAGCTTCA GCATGTGCAA GAAGAGAATG CTAGGCTGAA AAAGAGCAAA 
GAGCAACTGC TAAAAGAAGC TGAAGGTTGG AGTGAGAGGC ACACTGAGCT CACTGAGCAG 
ATCAAACTGT ATCGGAAGTC TCAGAAGGAC ATAGAAGAAG CACTTGCCTA CAAGGAAAAC 
GAAATTGAAG TTTTAACTAA CTGCATTATG CAGCTGAAGC AACTTGACAC AGATTCAGCA 
TCTGAGGCCA AGGACGGTGA AGGGCTTGAA TGGAGCACAA AAGATGATCT GGCCAACGGA 
GAGTTGCCAG ATAATGAGAA GATGAAGACT CAGATTAAGC AGATGATGGA TGCCTCCAGG 
GTAAAAACTA TGCTATCTCT AGTTGAAGAA GACAGAAATT CTCTGCAGTC CAAGCTGAGT 
GATGAAGTAG CAGCGAGACA TGAGCTGGAG GAGCAAATAA AAAAATTAGA ACACGACTCC 
TCTTCACTGC AGTCAGCCAA AGCTCGACTG GAAAATGAAT GTAAAACACT ACAACAGAAG 
GTGGAGATTC TTGGTGAACT TTACCAGCAG AAAGAAATGG CACTCCAGAA AAAGCTAACT 
CAAGAAGAAT ATGAGCGCCA GGAGAAGGAG CAGAAGTTGT CTGCCGCAGA TGAAAAGGCC 
GTGCTGGCTA TCGAGGAAGT GAAAGTTTAC AAGCAGAGGA TCCAAGATAT GGAAGAGGAA 
TTGCAAAAAA CAGAGAGATC TTATAAAAAC CAGATTGCTG CTCATGAGAA AAAAGCACAC 
GACAACTGGC TTATTGCCCG CTCTGCTGAG AGAGCTCTGG CTGAAGAGAA GAGAGAAGCA 
GCCAACCTGA GACAGAAATT AATGGAAGTG AACCAAAAAA CCATCATGCT TCAAAGACCG 
CTAATTGTAA AGCCAACTCC AGGCAGGCCT GATCGCCAGG TCCCACCACG ACGAGTGCCC 
TTAAGTAGAG ATGGTTCTTT TGGTCCATCG CCTGTGAGTG GAGGAAATCC ATCACCCACA 
CAGATGATGG AAGTTCCCAG TAGGCCCCTT TCTGCTCCTC AGAGAGAAGG CTCAAGAGCT 
GAATTTGGTA CAGTGGTAGA TGGCCCCCCT GCTCCACGGA GGCCACCAGA GCTCCCTGGA 
AGGATGTCCG TTCCTGATCT TGGGCCTGCT GTAGCGTCCC TGATCAGCAG TGGGCCGAGA 
ACCTCCTCTC CTGCCACAGC AAAGGATAGG GCGCCCTCTC CCAAAGAACC TGAAGCCCCC 
CGTGTAACTA CCGATTCACC TTCATCTATT GAACCAGCTA CAGTTAACGT CGGTCCCAAA 
GGCCCACCTT CTTTCCCTGG GACGCCCGTC ATGACCTCTC CAGTGCTGGG ACCTCCACCT 
CCACCACCTG TTAACTATGG TCCACCACCA GCTCCCTTTC CTGGACACTA CGGGCCTGGG 
CCTCGACCTC TCCCTGTGCC CCTAGTTTGT GGTGCTCCCT TACCACCACC AGCTGCAAGA 
GATTTCTTGC CTGGCCCATC CTTAGGAATA AGAGATCTGC CTCCTGGGCC ACTGCCACCC 
CTCCCAGACC CCAGAAGCTA CAGACGTGGG CCCCCTCATT TCCAGCCTCT AGGTCCTCCT 
AGCCCACATG ATTATCTGCC AGGCCCGCCG CTACCACCGC CTGCCTCCAG GGACTACGCA 
CCTTCTCGGA GCAGAGACTT GCCTCCAGCA GGACCCAGAG ACTACCCCGC AGGCCCCGCA 
CCGCCCCCGG CAGGCTCAAA AGACTACGCA CAGCCTCCAG TGCAGAAGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Physiological genomics25(3)514-24(2006 May)
Carre W,Wang X,Porter TE,Nys Y,Tang J,Bernberg E,Morgan R,Burnside J,Aggrey SE,Simon J,Cogburn LA

Expressed sequence tag analysis of Eimeria-stimulated intestinal intraepithelial lymphocytes in chickens.
Molecular biotechnology30(2)143-50(2005 Jun)
Min W,Lillehoj HS,Ashwell CM,van Tassell CP,Dalloul RA,Matukumalli LK,Han JY,Lillehoj EP

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

An expressed sequence tag database of T-cell-enriched activated chicken splenocytes: sequence analysis of 5251 clones.
Genomics66(2)144-51(2000 Jun)
Tirunagaru VG,Sofer L,Cui J,Burnside J

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MIA3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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