SEMA5A cDNA ORF clone, Gallus gallus(chicken)

The following SEMA5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa146212 XM_025147720.1
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa32234 XM_015282265.2
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282266.2
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282268.2
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_419016.6
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282264.2
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282267.2
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32235 XM_015282269.2
Latest version!
Gallus gallus semaphorin 5A (SEMA5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OGa32234 XM_015282265.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282266.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282267.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32235 XM_015282269.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_419016.5 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282268.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa32234 XM_015282264.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa146212
    Clone ID Related Accession (Same CDS sequence) XM_025147720.1
    Accession Version XM_025147720.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC 
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003488.1
    CDS16..3126
    Translation

    Target ORF information:

    RefSeq Version XM_025147720.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147720.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGCAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC 
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282264.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137750.1
    CDS253..3483
    Translation

    Target ORF information:

    RefSeq Version XM_015282264.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282265.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137751.1
    CDS436..3666
    Translation

    Target ORF information:

    RefSeq Version XM_015282265.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282265.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282268.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137754.1
    CDS196..3426
    Translation

    Target ORF information:

    RefSeq Version XM_015282268.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282266.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137752.1
    CDS294..3524
    Translation

    Target ORF information:

    RefSeq Version XM_015282266.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282266.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282267.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137753.1
    CDS178..3408
    Translation

    Target ORF information:

    RefSeq Version XM_015282267.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282267.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282265.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137751.1
    CDS727..3957
    Translation

    Target ORF information:

    RefSeq Version XM_015282265.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282265.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_419016.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419016.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_419016.4
    CDS830..4060
    Translation

    Target ORF information:

    RefSeq Version XM_419016.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_419016.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282266.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137752.1
    CDS260..3490
    Translation

    Target ORF information:

    RefSeq Version XM_015282266.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282267.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137753.1
    CDS181..3411
    Translation

    Target ORF information:

    RefSeq Version XM_015282267.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282267.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_419016.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_419016.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_419016.4
    CDS830..4060
    Translation

    Target ORF information:

    RefSeq Version XM_419016.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_419016.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282268.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137754.1
    CDS358..3588
    Translation

    Target ORF information:

    RefSeq Version XM_015282268.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282268.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32234
    Clone ID Related Accession (Same CDS sequence) XM_015282264.1 , XM_015282265.1 , XM_015282268.1 , XM_015282266.2 , XM_015282267.1 , XM_015282265.2 , XM_419016.5 , XM_015282266.1 , XM_015282267.2 , XM_419016.6 , XM_015282268.2 , XM_015282264.2
    Accession Version XM_015282264.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137750.1
    CDS452..3682
    Translation

    Target ORF information:

    RefSeq Version XM_015282264.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282264.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GGCATTTAAC
    AAAAACAACC TGATTCTGGA GGAGAGAAAC AAATACTTCA ATCCACATCT CACTGCAAAG
    ACTTACTCTA ATACCTATTT TACAGATTTC AATAATTATG ATGAATACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32235
    Clone ID Related Accession (Same CDS sequence) XM_015282269.2 , XM_015282269.1
    Accession Version XM_015282269.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GAAAAACTCT
    GATGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137755.1
    CDS455..3583
    Translation

    Target ORF information:

    RefSeq Version XM_015282269.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5A (SEMA5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GAAAAACTCT
    GATGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa32235
    Clone ID Related Accession (Same CDS sequence) XM_015282269.2 , XM_015282269.1
    Accession Version XM_015282269.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455787.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GAAAAACTCT
    GATGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137755.1
    CDS254..3382
    Translation

    Target ORF information:

    RefSeq Version XM_015282269.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCACCA TGAAAGGAAG CTGCCTTCTT GTACTGCTGT TGGCAGGATT TGGAGTATTA 
    GGAATACTGG AGCCAGATGC AGAAGATATC CACTGTCACA GAGCTGTGCA CCCAGTTATC
    ACATACAAAG AAATTGCTCC TTGGTTACAT GTGTTCAAAG CAGAAGATGC TGTAGACTTC
    TCACAATTAA CATTTGATCC AGGACAAAAA GAGCTTATTG CTGGAGCAAG AAACTACCTC
    TTCAGGTTGC AACTCGAGGA TCTCTCTCTA ATACAGGCAG TGGAATGGCA ATGTGATGAA
    GCTACTAAAA GAGCCTGTTA CAGTAAAGGA AAATCAAAGG AGGAATGCCA GAACTATATT
    CGAGTGCTTC TTGTTGGAGG CAACCATCTC TTTACCTGTG GAACCAATGC TTTCACTCCT
    ATTTGCACGA ATCGGACGTT AAATAACTTG ACAGAGATAC ATGATCAAAT CAGTGGCATG
    GCTCGTTGTC CTTATAGTCC CCAGCACAAC TCCACTGCAC TTCTCACTTC CAGTGGGGAA
    TTATATGCTG CTACAGCCAT GGATTTTCCG GGAAGGGATC CTGCCATTTA TCGCAGTTTG
    GGAGCCCTTC CTCCTCTCCG TACTGCACAG TACAACTCCA AGTGGCTGAA TGAACCCAAT
    TTTGTGTCAT CTTATGACAT TGGGAATTTC ACCTACTTCT TCTTCCGAGA GAATGCAGTG
    GAACATGACT GTGGAAAAAC AGTCTTCTCT CGTGCTGCTC GAGTGTGTAA AAATGATATT
    GGTGGCAAGT TTGTGCTCGA GGATACCTGG ACTACCTTCA TGAAAGCTCG GCTGAACTGC
    TCTCGTCCTG GAGAAATTCC ATTTTACTAT AATGAACTGC AGAGCACTTT CTTCCTGCCA
    GAATTGGACT TGATCTATGG GATTTTTACC ACTAATGTGA ACAGCATAGC AGCCTCAGCA
    GTTTGTGTTT TCAACTTGAG TGCCATAACT CAGGCTTTTA ATGGACCCTT CAAGTACCAG
    GAAAACTCTC GCTCTGCTTG GCTTCCATAT CCCAATCCTA ACCCAAATTT TCAGTGTGGC
    ACCATGGACC AGGGTTTGTA TGTGAACCTG ACTGAGAGAA ATCTTCAGGA TGCTCAAAAA
    TTCTTCTTAA TGCATGAGGT CGTCCAACCA GTTATTCCAA TTCCATACTT CATGGAAGAC
    AACAGCCGAT TTTCTCACGT GGTTGTGGAT GTTGTGCAGG GCAAGGACAT GCTCTTCCAT
    ATCATCTATC TTGCCACAGA CTATGGCACT ATTAAGAAAG TACTTGCCCC AATGAACCAG
    ACTTCAAGCA GCTGCCTGCT TGAGGAAATT GAACTCCTGC CAAAGAGTCA GCGGGAGCCC
    ATCAGGAGTC TTCAGATCCT GCACAGCCAG AGTGTCCTGT TTGTGGGCCT TCAGGAACAT
    GTGATTAAAG TCCCCCTGAA GAGATGTCTG TTTTACAAAA CATTCAGCGC ATGCATTGGA
    TCTCAGGATC CTTACTGTGG CTGGGACATG GTGATGAAGA AATGCACAAC TCTGGAAGAA
    AGTCTGAGCA TGAGTCAGTG GGAGCAAAGC ATTACTGTGT GTCCAACAAG GAATCTGACA
    GTGGATGGAC ATTTTGGAAC ATGGTCACAG TGGAAACCAT GCAGCCACAC GGACGGTGCC
    AGCACTGGCT CCTGCCTCTG CAGGACCCGT GTGTGCGACA GTCCAGCTCC TCAGTGCGGA
    GGGTGGCTAT GTGAAGGACC AACCATGGAA ATTGCCAACT GTTCCAGAAA TGGAGGCTGG
    ACACCATGGA CTTCTTGGTC ACCTTGTAGT ACCACCTGTG GAATAGGCTT TCAAGTTCGT
    CAGCGCTCCT GCAGCAATCC AACCCCTCGC CATGGAGGAC GTGTCTGTGT GGGACAGAAT
    CGTGAGGAGA GGTACTGCAA TGAGCATTTG TTGTGCCCCC CACATGTGTT TTGGACAAGC
    TGGGGTCCAT GGGAACGCTG CACGGCTCAG TGTGGAGGAG GGATTCAGGC TCGGCGTAGG
    ACATGTGAAA ATGGTCCTGA CTGTCCTGGC TGCAGTGTGG AATATCAACC TTGTAACACC
    AATGCCTGTC CAGAGTTAAA AAAGACAACT CCTTGGACAC CTTGGACTCC AGTTAATATT
    TCAGACAACG GTGGTCACTA TGAACAACGA TTCCGATACA CCTGCAAAGC ACGCTTGCCT
    GACCCAAATT TGCTGGAGGT GGGAAGGCAA AGGATTGAAA TGCGTTATTG TTCCAGCGAC
    GGCACCAGCG GGTGCTCGAC AGATGGATTA TCAGGGGATT TCTTGCGTTC TGGACGATAT
    TCTGCTCACA CCATCAATGG TGCCTGGTCG CCATGGTCTG CATGGTCTCA GTGCAGTCGT
    GACTGCAGTA GGGGTATACG AAATCGCAAA CGGGTCTGCA GCAATCCTGA GCCCAAGTAT
    GGTGGTCTTC CTTGCCTTGG TCCATCTCTA GAGTACCAGG AATGCAACAT TTTGCCTTGC
    CCAGTGGATG GAGGCTGGTC TTGCTGGTCA TCTTGGTCAA AGTGCTCAGC AACTTGTGGA
    GGAGGGCATT ACATGAGAAC CCGCTCCTGC ACCAATCCTG CACCGGCATA TGGAGGAGAT
    ATTTGTCTTG GCCTGCACAC TGAAGAAGCC CTCTGCAACA CACAACCATG TCCAGACAGC
    TGGTCAGAGT GGTCCGAGTG GTCTGAGTGC GACTCATCTG GCATGCAGCT CCGTGTTCGT
    CAGTGCATTC TTTTGTTTCC TGTGGGCAGC CAGTGCACGG GCAACACCAC AGAGAGCCGA
    GCCTGTGCTT TTGACTCCAA CTTTATACCA GAAATATCAG TGGCACGATC CAGCAGCAGA
    GAAGAAAAAC GATGTGGCGA GTTCAATATG TTTCACATGA TCGCCGTGGG ATTAAGTAGC
    TCAATACTTG GTTGCCTTCT TACTCTGCTT ATTTACACTT ACTGCCAACG ATATCAACAA
    CAGTCCCATG ATGCAACTGT CATCCATCCA GTGTCACCAG CTCCTCTAAA TACCAGCATC
    ACCAATCATA TCAACAAACT GGACAAATAT GATTCTGTAG AAGCCATCAA GAAAAACTCT
    GATGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.