SACM1L cDNA ORF clone, Gallus gallus(Chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SACM1L
Entrez Gene ID	420700
Full Name	SAC1 suppressor of actin mutations 1-like (yeast)
General protein information	Preferred Names SAC1 suppressor of actin mutations 1-like (yeast) Names phosphatidylinositide phosphatase SAC1
Gene Type	protein-coding
Organism	Gallus gallus(Chicken)
Summary	This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017].

mRNA and Protein(s)

mRNA	Protein	Name
XM_025147221.1	XP_025002989.1	phosphatidylinositide phosphatase SAC1 isoform X1
NM_001357508.1	NP_001344437.1	phosphatidylinositide phosphatase SAC1 isoform 1x
NM_001357507.1	NP_001344436.1	phosphatidylinositide phosphatase SAC1 isoform 1
XM_015281745.1	XP_015137231.1	phosphatidylinositide phosphatase SAC1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SACM1L	NP_001095601.1
Mus musculus (house mouse)	Sacm1l	NP_109617.1
Drosophila melanogaster (fruit fly)	Sac1	NP_001189016.1
Magnaporthe oryzae (rice blast fungus)	MGG_11279	XP_003714069.1
Arabidopsis thaliana (thale cress)	RHD4	NP_190714.2
Pan troglodytes (chimpanzee)	SACM1L	XP_516409.4
Caenorhabditis elegans (roundworm)	sac-1	NP_492518.1
Eremothecium gossypii	AGOS_AER447C	NP_985302.1
Gallus gallus (chicken)	SACM1L	XP_418799.4
Canis lupus familiaris (dog)	SACM1L	XP_541911.2
Danio rerio (zebrafish)	sacm1la	NP_001038343.1
Danio rerio (zebrafish)	sacm1lb	NP_001074093.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000891	XP_316868.5
Schizosaccharomyces pombe (fission yeast)	SPBC19F5.03	NP_596541.1
Arabidopsis thaliana (thale cress)	ATSAC1B	NP_201403.2
Homo sapiens (human)	SACM1L	NP_054735.3
	SACM1L	XP_001114514.1
Rattus norvegicus (Norway rat)	Sacm1l	NP_446250.1
Saccharomyces cerevisiae (baker's yeast)	SAC1	NP_012710.1
Kluyveromyces lactis	KLLA0B14388g	XP_452171.1
Neurospora crassa	NCU00896	XP_964812.1
Oryza sativa (rice)	Os02g0554300	NP_001047117.1
Oryza sativa (rice)	Os11g0309000	NP_001067755.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Loughran G, Chou MY, Ivanov IP, Jungreis I, Kellis M, Kiran AM, Baranov PV, Atkins JF
Nucleic acids research42(14)8928-38(2014 Aug)

The Sac1 phosphoinositide phosphatase regulates Golgi membrane morphology and mitotic spindle organization in mammals.
Liu Y, Boukhelifa M, Tribble E, Morin-Kensicki E, Uetrecht A, Bear JE, Bankaitis VA
Molecular biology of the cell19(7)3080-96(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SACM1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SACM1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa141643	NM_001357508.1 Latest version!	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa31765	NM_001357507.1 Latest version!	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa145871	XM_025147221.1 Latest version!	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OGa31765	XM_015281745.1 Latest version!	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SACM1L ( NM_001357508.1 ) cDNA ORF clone, Gallus gallus(Chicken) -> NP_001344437.1 Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa141643
Clone ID Related Accession (Same CDS sequence)	NM_001357508.1
Accession Version	NM_001357508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Gallus gallus(chicken)
Product	phosphatidylinositide phosphatase SAC1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000034.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGGACCGGC AGCGGCGGCG TCCTACGCCA ACCTGAAGCT GCATATAACA 
CCAGAGAAGT TCTATGTGGA AGCCTGTGAT GATGGAGCGG ATGATGTGCT GGCTATTGAC 
AGAGTCTCCA CAGAAGTCAC TCTTACAGTT AAAAAAGATG TTCCACCTTC AGCTGTCACA 
AGGCCTATAT TTGGTATTTT GGGAACAATA CGGTTGGTGG CAGGGACATA TCTTATTGTA 
ATAACAAAGA AGAAAAAAGT AGGTGAGATT TTCAGTCATG CAATCTGGAA AGCGACAGAC 
TTTGACATCC TCTCCTACAA AAAGACCATG CTGCACTTAA CTGATATTCA GTTACAGGAC 
AATAAAGTAT TTCTATCAAT GATTAGTCAC GTTTTGAGCG TGGATGGGTT TTACTTCTCA 
ACAACTTATG ACCTAACACA CACCCTGCAA CGATTAGCCA ACACCAGCCC AGAGTTCCAG 
GAGATGAGCC TATTGGAGAG GGCAGATCCA CGGTTTGTTT GGAACGGACA TCTTCTTAGA 
GAATTTGCTG CTCAACCAGA GATCCATAGG TTTGCTACAC CAGTGATGCA TGGATTTATC 
TCTATGCACT CTTGTTCTAT TAATGGCAAG TGTTTTGACT GGCTGCTTGT TTCCAGAAGA 
AGCTGTTTCA GAGCTGGTGT ACGCTACTAT GTAAGAGGTA TTGATTCAGA AGGCCATGCA 
GCTAACTTTG TTGAAACAGA ACAAATTGTG CATTACAAGG GGAGCAAAGC ATCGTTTGTT 
CAGACACGTG GATCAATTCC TTTCTTCTGG TCTCAGAGAC CAAACCTCAA GTATAAACCA 
AAGCCGCAGA TCAGCAAGTC AGTAAATCAT ATGGATGGCT TCCAAAGACA CTTTGACTCA 
CAAATCATCA GCTACGGAAA GCAGATGATT GTTAACTTGG TTAACCAGAA GGGTTCTGAG 
CTGCCTCTGG AGCAGACATT TGCAAAAATG GTGAATAGCA TGGCAAATGG AATGGTCAGA 
TACATAGCAT TTGACTTCCA TAAAGAGTGC AGTCGGATGA GATGGGATCG ACTTCAGATT 
TTACTGGATC AACTAGCAGA ACAGCAGGAT GAGTTTAGTT ATTTTCTAGT TGATTCGGAC 
GGTAAAATCG TGACTCAGCA GGAAGGAACA TTCCGCAGTA ACTGTATGGA CTGCCTGGAT 
AGGACAAATG TGATTCAGAG CTTGTTAGCA CGCCGATCTC TTCAAGCCCA GCTTCAGAGG 
CTAGGTGTTT TGCATGTTGG ACAGAGAATT GAGGAGCAAG CAGACTTTGA GAAAATCTAC 
AAAAATGCAT GGGCTGATAA TGCTAATGCT TGTGCCAAAC AGTATGCTGG AACTGGTGCC 
TTAAAGACAG ATTACACAAG AACTGGGAAG AGGACTCAGT GGGGCTTAAT AATGGATGGC 
TGGAACTCAT TAATACGTTA TTACAAAAAT AACTTTTCTG ATGGATTTAG ACAAGATGCT 
ATTGATCTCT TCCTTGGAAA TTATTCAGTG GATGAAGTAG AATCTGCTAG TCCTCTACAT 
GTCAAGAAAG ATTGGAAGTT CTTAGCTTTG CCTATTATTA TGGTGGTTGC TTTCTCCATG 
TGCATCATTT GTTTGCTAAT GGCTGGTGAT ACATGGACTG AGACACTGGC CTACGTGCTT 
TTCTGGGGAA CTGCAAGCTT TGGAACTTTT GCCATCATCC TTTACAACGG CAAGGATTTT 
GTAGATGCAC CCAAGCTGGT CCAGAAAGAG AAGATGGACT GAATTTGTGT GTGTGGAAAG 
CGGCTTGGTA CTGAGGATTC CTCAAGCCAC ACTTGGAGTC TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344437.1
CDS	30..1877
Translation	MAAAGPAAAASYANLKLHITPEKFYVEACDDGADDVLAIDRVSTEVTLTVKKDVPPSAVTRPIFGILGTIRLVAGTYLIVITKKKKVGEIFSHAIWKATDFDILSYKKTMLHLTDIQLQDNKVFLSMISHVLSVDGFYFSTTYDLTHTLQRLANTSPEFQEMSLLERADPRFVWNGHLLREFAAQPEIHRFATPVMHGFISMHSCSINGKCFDWLLVSRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYKGSKASFVQTRGSIPFFWSQRPNLKYKPKPQISKSVNHMDGFQRHFDSQIISYGKQMIVNLVNQKGSELPLEQTFAKMVNSMANGMVRYIAFDFHKECSRMRWDRLQILLDQLAEQQDEFSYFLVDSDGKIVTQQEGTFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLIMDGWNSLIRYYKNNFSDGFRQDAIDLFLGNYSVDEVESASPLHVKKDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASFGTFAIILYNGKDFVDAPKLVQKEKMD*ICVCGKRLGTEDSSSHTWSLY

Target ORF information:

RefSeq Version	NM_001357508.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357508.1

ORF Insert Sequence:

ATGGCGGCGG CGGGACCGGC AGCGGCGGCG TCCTACGCCA ACCTGAAGCT GCATATAACA 
CCAGAGAAGT TCTATGTGGA AGCCTGTGAT GATGGAGCGG ATGATGTGCT GGCTATTGAC 
AGAGTCTCCA CAGAAGTCAC TCTTACAGTT AAAAAAGATG TTCCACCTTC AGCTGTCACA 
AGGCCTATAT TTGGTATTTT GGGAACAATA CGGTTGGTGG CAGGGACATA TCTTATTGTA 
ATAACAAAGA AGAAAAAAGT AGGTGAGATT TTCAGTCATG CAATCTGGAA AGCGACAGAC 
TTTGACATCC TCTCCTACAA AAAGACCATG CTGCACTTAA CTGATATTCA GTTACAGGAC 
AATAAAGTAT TTCTATCAAT GATTAGTCAC GTTTTGAGCG TGGATGGGTT TTACTTCTCA 
ACAACTTATG ACCTAACACA CACCCTGCAA CGATTAGCCA ACACCAGCCC AGAGTTCCAG 
GAGATGAGCC TATTGGAGAG GGCAGATCCA CGGTTTGTTT GGAACGGACA TCTTCTTAGA 
GAATTTGCTG CTCAACCAGA GATCCATAGG TTTGCTACAC CAGTGATGCA TGGATTTATC 
TCTATGCACT CTTGTTCTAT TAATGGCAAG TGTTTTGACT GGCTGCTTGT TTCCAGAAGA 
AGCTGTTTCA GAGCTGGTGT ACGCTACTAT GTAAGAGGTA TTGATTCAGA AGGCCATGCA 
GCTAACTTTG TTGAAACAGA ACAAATTGTG CATTACAAGG GGAGCAAAGC ATCGTTTGTT 
CAGACACGTG GATCAATTCC TTTCTTCTGG TCTCAGAGAC CAAACCTCAA GTATAAACCA 
AAGCCGCAGA TCAGCAAGTC AGTAAATCAT ATGGATGGCT TCCAAAGACA CTTTGACTCA 
CAAATCATCA GCTACGGAAA GCAGATGATT GTTAACTTGG TTAACCAGAA GGGTTCTGAG 
CTGCCTCTGG AGCAGACATT TGCAAAAATG GTGAATAGCA TGGCAAATGG AATGGTCAGA 
TACATAGCAT TTGACTTCCA TAAAGAGTGC AGTCGGATGA GATGGGATCG ACTTCAGATT 
TTACTGGATC AACTAGCAGA ACAGCAGGAT GAGTTTAGTT ATTTTCTAGT TGATTCGGAC 
GGTAAAATCG TGACTCAGCA GGAAGGAACA TTCCGCAGTA ACTGTATGGA CTGCCTGGAT 
AGGACAAATG TGATTCAGAG CTTGTTAGCA CGCCGATCTC TTCAAGCCCA GCTTCAGAGG 
CTAGGTGTTT TGCATGTTGG ACAGAGAATT GAGGAGCAAG CAGACTTTGA GAAAATCTAC 
AAAAATGCAT GGGCTGATAA TGCTAATGCT TGTGCCAAAC AGTATGCTGG AACTGGTGCC 
TTAAAGACAG ATTACACAAG AACTGGGAAG AGGACTCAGT GGGGCTTAAT AATGGATGGC 
TGGAACTCAT TAATACGTTA TTACAAAAAT AACTTTTCTG ATGGATTTAG ACAAGATGCT 
ATTGATCTCT TCCTTGGAAA TTATTCAGTG GATGAAGTAG AATCTGCTAG TCCTCTACAT 
GTCAAGAAAG ATTGGAAGTT CTTAGCTTTG CCTATTATTA TGGTGGTTGC TTTCTCCATG 
TGCATCATTT GTTTGCTAAT GGCTGGTGAT ACATGGACTG AGACACTGGC CTACGTGCTT 
TTCTGGGGAA CTGCAAGCTT TGGAACTTTT GCCATCATCC TTTACAACGG CAAGGATTTT 
GTAGATGCAC CCAAGCTGGT CCAGAAAGAG AAGATGGACT GAATTTGTGT GTGTGGAAAG 
CGGCTTGGTA CTGAGGATTC CTCAAGCCAC ACTTGGAGTC TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( NM_001357507.1 ) cDNA ORF clone, Gallus gallus(Chicken) -> NP_001344436.1 Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa31765
Clone ID Related Accession (Same CDS sequence)	NM_001357507.1 , XM_015281745.1
Accession Version	NM_001357507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Gallus gallus(chicken)
Product	phosphatidylinositide phosphatase SAC1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000034.1. On Oct 28, 2017 this sequence version replaced XM_015281745.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGGACCGGC AGCGGCGGCG TCCTACGCCA ACCTGAAGCT GCATATAACA 
CCAGAGAAGT TCTATGTGGA AGCCTGTGAT GATGGAGCGG ATGATGTGCT GGCTATTGAC 
AGAGTCTCCA CAGAAGTCAC TCTTACAGTT AAAAAAGATG TTCCACCTTC AGCTGTCACA 
AGGCCTATAT TTGGTATTTT GGGAACAATA CGGTTGGTGG CAGGGACATA TCTTATTGTA 
ATAACAAAGA AGAAAAAAGT AGGTGAGATT TTCAGTCATG CAATCTGGAA AGCGACAGAC 
TTTGACATCC TCTCCTACAA AAAGACCATG CTGCACTTAA CTGATATTCA GTTACAGGAC 
AATAAAGTAT TTCTATCAAT GATTAGTCAC GTTTTGAGCG TGGATGGGTT TTACTTCTCA 
ACAACTTATG ACCTAACACA CACCCTGCAA CGATTAGCCA ACACCAGCCC AGAGTTCCAG 
GAGATGAGCC TATTGGAGAG GGCAGATCCA CGGTTTGTTT GGAACGGACA TCTTCTTAGA 
GAATTTGCTG CTCAACCAGA GATCCATAGG TTTGCTACAC CAGTGATGCA TGGATTTATC 
TCTATGCACT CTTGTTCTAT TAATGGCAAG TGTTTTGACT GGCTGCTTGT TTCCAGAAGA 
AGCTGTTTCA GAGCTGGTGT ACGCTACTAT GTAAGAGGTA TTGATTCAGA AGGCCATGCA 
GCTAACTTTG TTGAAACAGA ACAAATTGTG CATTACAAGG GGAGCAAAGC ATCGTTTGTT 
CAGACACGTG GATCAATTCC TTTCTTCTGG TCTCAGAGAC CAAACCTCAA GTATAAACCA 
AAGCCGCAGA TCAGCAAGTC AGTAAATCAT ATGGATGGCT TCCAAAGACA CTTTGACTCA 
CAAATCATCA GCTACGGAAA GCAGATGATT GTTAACTTGG TTAACCAGAA GGGTTCTGAG 
CTGCCTCTGG AGCAGACATT TGCAAAAATG GTGAATAGCA TGGCAAATGG AATGGTCAGA 
TACATAGCAT TTGACTTCCA TAAAGAGTGC AGTCGGATGA GATGGGATCG ACTTCAGATT 
TTACTGGATC AACTAGCAGA ACAGCAGGAT GAGTTTAGTT ATTTTCTAGT TGATTCGGAC 
GGTAAAATCG TGACTCAGCA GGAAGGAACA TTCCGCAGTA ACTGTATGGA CTGCCTGGAT 
AGGACAAATG TGATTCAGAG CTTGTTAGCA CGCCGATCTC TTCAAGCCCA GCTTCAGAGG 
CTAGGTGTTT TGCATGTTGG ACAGAGAATT GAGGAGCAAG CAGACTTTGA GAAAATCTAC 
AAAAATGCAT GGGCTGATAA TGCTAATGCT TGTGCCAAAC AGTATGCTGG AACTGGTGCC 
TTAAAGACAG ATTACACAAG AACTGGGAAG AGGACTCAGT GGGGCTTAAT AATGGATGGC 
TGGAACTCAT TAATACGTTA TTACAAAAAT AACTTTTCTG ATGGATTTAG ACAAGATGCT 
ATTGATCTCT TCCTTGGAAA TTATTCAGTG GATGAAGTAG AATCTGCTAG TCCTCTACAT 
GTCAAGAAAG ATTGGAAGTT CTTAGCTTTG CCTATTATTA TGGTGGTTGC TTTCTCCATG 
TGCATCATTT GTTTGCTAAT GGCTGGTGAT ACATGGACTG AGACACTGGC CTACGTGCTT 
TTCTGGGGAA CTGCAAGCTT TGGAACTTTT GCCATCATCC TTTACAACGG CAAGGATTTT 
GTAGATGCAC CCAAGCTGGT CCAGAAAGAG AAGATGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344436.1
CDS	30..1811
Translation	MAAAGPAAAASYANLKLHITPEKFYVEACDDGADDVLAIDRVSTEVTLTVKKDVPPSAVTRPIFGILGTIRLVAGTYLIVITKKKKVGEIFSHAIWKATDFDILSYKKTMLHLTDIQLQDNKVFLSMISHVLSVDGFYFSTTYDLTHTLQRLANTSPEFQEMSLLERADPRFVWNGHLLREFAAQPEIHRFATPVMHGFISMHSCSINGKCFDWLLVSRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYKGSKASFVQTRGSIPFFWSQRPNLKYKPKPQISKSVNHMDGFQRHFDSQIISYGKQMIVNLVNQKGSELPLEQTFAKMVNSMANGMVRYIAFDFHKECSRMRWDRLQILLDQLAEQQDEFSYFLVDSDGKIVTQQEGTFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLIMDGWNSLIRYYKNNFSDGFRQDAIDLFLGNYSVDEVESASPLHVKKDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASFGTFAIILYNGKDFVDAPKLVQKEKMD

Target ORF information:

RefSeq Version	NM_001357507.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357507.1

ORF Insert Sequence:

ATGGCGGCGG CGGGACCGGC AGCGGCGGCG TCCTACGCCA ACCTGAAGCT GCATATAACA 
CCAGAGAAGT TCTATGTGGA AGCCTGTGAT GATGGAGCGG ATGATGTGCT GGCTATTGAC 
AGAGTCTCCA CAGAAGTCAC TCTTACAGTT AAAAAAGATG TTCCACCTTC AGCTGTCACA 
AGGCCTATAT TTGGTATTTT GGGAACAATA CGGTTGGTGG CAGGGACATA TCTTATTGTA 
ATAACAAAGA AGAAAAAAGT AGGTGAGATT TTCAGTCATG CAATCTGGAA AGCGACAGAC 
TTTGACATCC TCTCCTACAA AAAGACCATG CTGCACTTAA CTGATATTCA GTTACAGGAC 
AATAAAGTAT TTCTATCAAT GATTAGTCAC GTTTTGAGCG TGGATGGGTT TTACTTCTCA 
ACAACTTATG ACCTAACACA CACCCTGCAA CGATTAGCCA ACACCAGCCC AGAGTTCCAG 
GAGATGAGCC TATTGGAGAG GGCAGATCCA CGGTTTGTTT GGAACGGACA TCTTCTTAGA 
GAATTTGCTG CTCAACCAGA GATCCATAGG TTTGCTACAC CAGTGATGCA TGGATTTATC 
TCTATGCACT CTTGTTCTAT TAATGGCAAG TGTTTTGACT GGCTGCTTGT TTCCAGAAGA 
AGCTGTTTCA GAGCTGGTGT ACGCTACTAT GTAAGAGGTA TTGATTCAGA AGGCCATGCA 
GCTAACTTTG TTGAAACAGA ACAAATTGTG CATTACAAGG GGAGCAAAGC ATCGTTTGTT 
CAGACACGTG GATCAATTCC TTTCTTCTGG TCTCAGAGAC CAAACCTCAA GTATAAACCA 
AAGCCGCAGA TCAGCAAGTC AGTAAATCAT ATGGATGGCT TCCAAAGACA CTTTGACTCA 
CAAATCATCA GCTACGGAAA GCAGATGATT GTTAACTTGG TTAACCAGAA GGGTTCTGAG 
CTGCCTCTGG AGCAGACATT TGCAAAAATG GTGAATAGCA TGGCAAATGG AATGGTCAGA 
TACATAGCAT TTGACTTCCA TAAAGAGTGC AGTCGGATGA GATGGGATCG ACTTCAGATT 
TTACTGGATC AACTAGCAGA ACAGCAGGAT GAGTTTAGTT ATTTTCTAGT TGATTCGGAC 
GGTAAAATCG TGACTCAGCA GGAAGGAACA TTCCGCAGTA ACTGTATGGA CTGCCTGGAT 
AGGACAAATG TGATTCAGAG CTTGTTAGCA CGCCGATCTC TTCAAGCCCA GCTTCAGAGG 
CTAGGTGTTT TGCATGTTGG ACAGAGAATT GAGGAGCAAG CAGACTTTGA GAAAATCTAC 
AAAAATGCAT GGGCTGATAA TGCTAATGCT TGTGCCAAAC AGTATGCTGG AACTGGTGCC 
TTAAAGACAG ATTACACAAG AACTGGGAAG AGGACTCAGT GGGGCTTAAT AATGGATGGC 
TGGAACTCAT TAATACGTTA TTACAAAAAT AACTTTTCTG ATGGATTTAG ACAAGATGCT 
ATTGATCTCT TCCTTGGAAA TTATTCAGTG GATGAAGTAG AATCTGCTAG TCCTCTACAT 
GTCAAGAAAG ATTGGAAGTT CTTAGCTTTG CCTATTATTA TGGTGGTTGC TTTCTCCATG 
TGCATCATTT GTTTGCTAAT GGCTGGTGAT ACATGGACTG AGACACTGGC CTACGTGCTT 
TTCTGGGGAA CTGCAAGCTT TGGAACTTTT GCCATCATCC TTTACAACGG CAAGGATTTT 
GTAGATGCAC CCAAGCTGGT CCAGAAAGAG AAGATGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( XM_025147221.1 ) cDNA ORF clone, Gallus gallus(Chicken) -> XP_025002989.1 Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa145871
Clone ID Related Accession (Same CDS sequence)	XM_025147221.1
Accession Version	XM_025147221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphatidylinositide phosphatase SAC1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGC CTGTGATGAT GGAGCGGATG ATGTGCTGGC TATTGACAGA GTCTCCACAG 
AAGTCACTCT TACAGTTAAA AAAGATGTTC CACCTTCAGC TGTCACAAGG CCTATATTTG 
GTATTTTGGG AACAATACGG TTGGTGGCAG TTACAGGACA ATAAAGTATT TCTATCAATG 
ATTAGTCACG TTTTGAGCGT GGATGGGTTT TACTTCTCAA CAACTTATGA CCTAACACAC 
ACCCTGCAAC GATTAGCCAA CACCAGCCCA GAGTTCCAGG AGATGAGCCT ATTGGAGAGG 
GCAGATCCAC GGTTTGTTTG GAACGGACAT CTTCTTAGAG AATTTGCTGC TCAACCAGAG 
ATCCATAGGT TTGCTACACC AGTGATGCAT GGATTTATCT CTATGCACTC TTGTTCTATT 
AATGGCAAGT GTTTTGACTG GCTGCTTGTT TCCAGAAGAA GCTGTTTCAG AGCTGGTGTA 
CGCTACTATG TAAGAGGTAT TGATTCAGAA GGCCATGCAG CTAACTTTGT TGAAACAGAA 
CAAATTGTGC ATTACAAGGG GAGCAAAGCA TCGTTTGTTC AGACACGTGG ATCAATTCCT 
TTCTTCTGGT CTCAGAGACC AAACCTCAAG TATAAACCAA AGCCGCAGAT CAGCAAGTCA 
GTAAATCATA TGGATGGCTT CCAAAGACAC TTTGACTCAC AAATCATCAG CTACGGAAAG 
CAGATGATTG TTAACTTGGT TAACCAGAAG GGTTCTGAGC TGCCTCTGGA GCAGACATTT 
GCAAAAATGG TGAATAGCAT GGCAAATGGA ATGGTCAGAT ACATAGCATT TGACTTCCAT 
AAAGAGTGCA GTCGGATGAG ATGGGATCGA CTTCAGATTT TACTGGATCA ACTAGCAGAA 
CAGCAGGATG AGTTTAGTTA TTTTCTAGTT GATTCGGACG GTAAAATCGT GACTCAGCAG 
GAAGGAACAT TCCGCAGTAA CTGTATGGAC TGCCTGGATA GGACAAATGT GATTCAGAGC 
TTGTTAGCAC GCCGATCTCT TCAAGCCCAG CTTCAGAGGC TAGGTGTTTT GCATGTTGGA 
CAGAGAATTG AGGAGCAAGC AGACTTTGAG AAAATCTACA AAAATGCATG GGCTGATAAT 
GCTAATGCTT GTGCCAAACA GTATGCTGGA ACTGGTGCCT TAAAGACAGA TTACACAAGA 
ACTGGGAAGA GGACTCAGTG GGGCTTAATA ATGGATGGCT GGAACTCATT AATACGTTAT 
TACAAAAATA ACTTTTCTGA TGGATTTAGA CAAGATGCTA TTGATCTCTT CCTTGGAAAT 
TATTCAGTGG ATGAAGTAGA ATCTGCTAGT CCTCTACATG TCAAGAAAGA TTGGAAGTTC 
TTAGCTTTGC CTATTATTAT GGTGGTTGCT TTCTCCATGT GCATCATTTG TTTGCTAATG 
GCTGGTGATA CATGGACTGA GACACTGGCC TACGTGCTTT TCTGGGGAAC TGCAAGCTTT 
GGAACTTTTG CCATCATCCT TTACAACGGC AAGGATTTTG TAGATGCACC CAAGCTGGTC 
CAGAAAGAGA AGATGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002989.1
CDS	24..1604
Translation	MWKPVMMERMMCWLLTESPQKSLLQLKKMFHLQLSQGLYLVFWEQYGWWQLQDNKVFLSMISHVLSVDGFYFSTTYDLTHTLQRLANTSPEFQEMSLLERADPRFVWNGHLLREFAAQPEIHRFATPVMHGFISMHSCSINGKCFDWLLVSRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYKGSKASFVQTRGSIPFFWSQRPNLKYKPKPQISKSVNHMDGFQRHFDSQIISYGKQMIVNLVNQKGSELPLEQTFAKMVNSMANGMVRYIAFDFHKECSRMRWDRLQILLDQLAEQQDEFSYFLVDSDGKIVTQQEGTFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLIMDGWNSLIRYYKNNFSDGFRQDAIDLFLGNYSVDEVESASPLHVKKDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASFGTFAIILYNGKDFVDAPKLVQKEKMD

Target ORF information:

RefSeq Version	XM_025147221.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147221.1

ORF Insert Sequence:

ATGTGGAAGC CTGTGATGAT GGAGCGGATG ATGTGCTGGC TATTGACAGA GTCTCCACAG 
AAGTCACTCT TACAGTTAAA AAAGATGTTC CACCTTCAGC TGTCACAAGG CCTATATTTG 
GTATTTTGGG AACAATACGG TTGGTGGCAG TTACAGGACA ATAAAGTATT TCTATCAATG 
ATTAGTCACG TTTTGAGCGT GGATGGGTTT TACTTCTCAA CAACTTATGA CCTAACACAC 
ACCCTGCAAC GATTAGCCAA CACCAGCCCA GAGTTCCAGG AGATGAGCCT ATTGGAGAGG 
GCAGATCCAC GGTTTGTTTG GAACGGACAT CTTCTTAGAG AATTTGCTGC TCAACCAGAG 
ATCCATAGGT TTGCTACACC AGTGATGCAT GGATTTATCT CTATGCACTC TTGTTCTATT 
AATGGCAAGT GTTTTGACTG GCTGCTTGTT TCCAGAAGAA GCTGTTTCAG AGCTGGTGTA 
CGCTACTATG TAAGAGGTAT TGATTCAGAA GGCCATGCAG CTAACTTTGT TGAAACAGAA 
CAAATTGTGC ATTACAAGGG GAGCAAAGCA TCGTTTGTTC AGACACGTGG ATCAATTCCT 
TTCTTCTGGT CTCAGAGACC AAACCTCAAG TATAAACCAA AGCCGCAGAT CAGCAAGTCA 
GTAAATCATA TGGATGGCTT CCAAAGACAC TTTGACTCAC AAATCATCAG CTACGGAAAG 
CAGATGATTG TTAACTTGGT TAACCAGAAG GGTTCTGAGC TGCCTCTGGA GCAGACATTT 
GCAAAAATGG TGAATAGCAT GGCAAATGGA ATGGTCAGAT ACATAGCATT TGACTTCCAT 
AAAGAGTGCA GTCGGATGAG ATGGGATCGA CTTCAGATTT TACTGGATCA ACTAGCAGAA 
CAGCAGGATG AGTTTAGTTA TTTTCTAGTT GATTCGGACG GTAAAATCGT GACTCAGCAG 
GAAGGAACAT TCCGCAGTAA CTGTATGGAC TGCCTGGATA GGACAAATGT GATTCAGAGC 
TTGTTAGCAC GCCGATCTCT TCAAGCCCAG CTTCAGAGGC TAGGTGTTTT GCATGTTGGA 
CAGAGAATTG AGGAGCAAGC AGACTTTGAG AAAATCTACA AAAATGCATG GGCTGATAAT 
GCTAATGCTT GTGCCAAACA GTATGCTGGA ACTGGTGCCT TAAAGACAGA TTACACAAGA 
ACTGGGAAGA GGACTCAGTG GGGCTTAATA ATGGATGGCT GGAACTCATT AATACGTTAT 
TACAAAAATA ACTTTTCTGA TGGATTTAGA CAAGATGCTA TTGATCTCTT CCTTGGAAAT 
TATTCAGTGG ATGAAGTAGA ATCTGCTAGT CCTCTACATG TCAAGAAAGA TTGGAAGTTC 
TTAGCTTTGC CTATTATTAT GGTGGTTGCT TTCTCCATGT GCATCATTTG TTTGCTAATG 
GCTGGTGATA CATGGACTGA GACACTGGCC TACGTGCTTT TCTGGGGAAC TGCAAGCTTT 
GGAACTTTTG CCATCATCCT TTACAACGGC AAGGATTTTG TAGATGCACC CAAGCTGGTC 
CAGAAAGAGA AGATGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( XM_015281745.1 ) cDNA ORF clone, Gallus gallus(Chicken) -> XP_015137231.1 Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), mRNA.

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CloneID	OGa31765
Clone ID Related Accession (Same CDS sequence)	NM_001357507.1 , XM_015281745.1
Accession Version	XM_015281745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(Chicken)
Product	phosphatidylinositide phosphatase SAC1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGACCGGC AGCGGCGGCG TCCTACGCCA ACCTGAAGCT GCATATAACA 
CCAGAGAAGT TCTATGTGGA AGCCTGTGAT GATGGAGCGG ATGATGTGCT GGCTATTGAC 
AGAGTCTCCA CAGAAGTCAC TCTTACAGTT AAAAAAGATG TTCCACCTTC AGCTGTCACA 
AGGCCTATAT TTGGTATTTT GGGAACAATA CGGTTGGTGG CAGGGACATA TCTTATTGTA 
ATAACAAAGA AGAAAAAAGT AGGTGAGATT TTCAGTCATG CAATCTGGAA AGCGACAGAC 
TTTGACATCC TCTCCTACAA AAAGACCATG CTGCACTTAA CTGATATTCA GTTACAGGAC 
AATAAAGTAT TTCTATCAAT GATTAGTCAC GTTTTGAGCG TGGATGGGTT TTACTTCTCA 
ACAACTTATG ACCTAACACA CACCCTGCAA CGATTAGCCA ACACCAGCCC AGAGTTCCAG 
GAGATGAGCC TATTGGAGAG GGCAGATCCA CGGTTTGTTT GGAACGGACA TCTTCTTAGA 
GAATTTGCTG CTCAACCAGA GATCCATAGG TTTGCTACAC CAGTGATGCA TGGATTTATC 
TCTATGCACT CTTGTTCTAT TAATGGCAAG TGTTTTGACT GGCTGCTTGT TTCCAGAAGA 
AGCTGTTTCA GAGCTGGTGT ACGCTACTAT GTAAGAGGTA TTGATTCAGA AGGCCATGCA 
GCTAACTTTG TTGAAACAGA ACAAATTGTG CATTACAAGG GGAGCAAAGC ATCGTTTGTT 
CAGACACGTG GATCAATTCC TTTCTTCTGG TCTCAGAGAC CAAACCTCAA GTATAAACCA 
AAGCCGCAGA TCAGCAAGTC AGTAAATCAT ATGGATGGCT TCCAAAGACA CTTTGACTCA 
CAAATCATCA GCTACGGAAA GCAGATGATT GTTAACTTGG TTAACCAGAA GGGTTCTGAG 
CTGCCTCTGG AGCAGACATT TGCAAAAATG GTGAATAGCA TGGCAAATGG AATGGTCAGA 
TACATAGCAT TTGACTTCCA TAAAGAGTGC AGTCGGATGA GATGGGATCG ACTTCAGATT 
TTACTGGATC AACTAGCAGA ACAGCAGGAT GAGTTTAGTT ATTTTCTAGT TGATTCGGAC 
GGTAAAATCG TGACTCAGCA GGAAGGAACA TTCCGCAGTA ACTGTATGGA CTGCCTGGAT 
AGGACAAATG TGATTCAGAG CTTGTTAGCA CGCCGATCTC TTCAAGCCCA GCTTCAGAGG 
CTAGGTGTTT TGCATGTTGG ACAGAGAATT GAGGAGCAAG CAGACTTTGA GAAAATCTAC 
AAAAATGCAT GGGCTGATAA TGCTAATGCT TGTGCCAAAC AGTATGCTGG AACTGGTGCC 
TTAAAGACAG ATTACACAAG AACTGGGAAG AGGACTCAGT GGGGCTTAAT AATGGATGGC 
TGGAACTCAT TAATACGTTA TTACAAAAAT AACTTTTCTG ATGGATTTAG ACAAGATGCT 
ATTGATCTCT TCCTTGGAAA TTATTCAGTG GATGAAGTAG AATCTGCTAG TCCTCTACAT 
GTCAAGAAAG ATTGGAAGTT CTTAGCTTTG CCTATTATTA TGGTGGTTGC TTTCTCCATG 
TGCATCATTT GTTTGCTAAT GGCTGGTGAT ACATGGACTG AGACACTGGC CTACGTGCTT 
TTCTGGGGAA CTGCAAGCTT TGGAACTTTT GCCATCATCC TTTACAACGG CAAGGATTTT 
GTAGATGCAC CCAAGCTGGT CCAGAAAGAG AAGATGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137231.1
CDS	121..1902
Translation	MAAAGPAAAASYANLKLHITPEKFYVEACDDGADDVLAIDRVSTEVTLTVKKDVPPSAVTRPIFGILGTIRLVAGTYLIVITKKKKVGEIFSHAIWKATDFDILSYKKTMLHLTDIQLQDNKVFLSMISHVLSVDGFYFSTTYDLTHTLQRLANTSPEFQEMSLLERADPRFVWNGHLLREFAAQPEIHRFATPVMHGFISMHSCSINGKCFDWLLVSRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYKGSKASFVQTRGSIPFFWSQRPNLKYKPKPQISKSVNHMDGFQRHFDSQIISYGKQMIVNLVNQKGSELPLEQTFAKMVNSMANGMVRYIAFDFHKECSRMRWDRLQILLDQLAEQQDEFSYFLVDSDGKIVTQQEGTFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLIMDGWNSLIRYYKNNFSDGFRQDAIDLFLGNYSVDEVESASPLHVKKDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASFGTFAIILYNGKDFVDAPKLVQKEKMD

Target ORF information:

RefSeq Version	XM_015281745.1
Organism	Gallus gallus(Chicken)
Definition	Gallus gallus SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281745.1

ORF Insert Sequence:

ATGGCGGCGG CGGGACCGGC AGCGGCGGCG TCCTACGCCA ACCTGAAGCT GCATATAACA 
CCAGAGAAGT TCTATGTGGA AGCCTGTGAT GATGGAGCGG ATGATGTGCT GGCTATTGAC 
AGAGTCTCCA CAGAAGTCAC TCTTACAGTT AAAAAAGATG TTCCACCTTC AGCTGTCACA 
AGGCCTATAT TTGGTATTTT GGGAACAATA CGGTTGGTGG CAGGGACATA TCTTATTGTA 
ATAACAAAGA AGAAAAAAGT AGGTGAGATT TTCAGTCATG CAATCTGGAA AGCGACAGAC 
TTTGACATCC TCTCCTACAA AAAGACCATG CTGCACTTAA CTGATATTCA GTTACAGGAC 
AATAAAGTAT TTCTATCAAT GATTAGTCAC GTTTTGAGCG TGGATGGGTT TTACTTCTCA 
ACAACTTATG ACCTAACACA CACCCTGCAA CGATTAGCCA ACACCAGCCC AGAGTTCCAG 
GAGATGAGCC TATTGGAGAG GGCAGATCCA CGGTTTGTTT GGAACGGACA TCTTCTTAGA 
GAATTTGCTG CTCAACCAGA GATCCATAGG TTTGCTACAC CAGTGATGCA TGGATTTATC 
TCTATGCACT CTTGTTCTAT TAATGGCAAG TGTTTTGACT GGCTGCTTGT TTCCAGAAGA 
AGCTGTTTCA GAGCTGGTGT ACGCTACTAT GTAAGAGGTA TTGATTCAGA AGGCCATGCA 
GCTAACTTTG TTGAAACAGA ACAAATTGTG CATTACAAGG GGAGCAAAGC ATCGTTTGTT 
CAGACACGTG GATCAATTCC TTTCTTCTGG TCTCAGAGAC CAAACCTCAA GTATAAACCA 
AAGCCGCAGA TCAGCAAGTC AGTAAATCAT ATGGATGGCT TCCAAAGACA CTTTGACTCA 
CAAATCATCA GCTACGGAAA GCAGATGATT GTTAACTTGG TTAACCAGAA GGGTTCTGAG 
CTGCCTCTGG AGCAGACATT TGCAAAAATG GTGAATAGCA TGGCAAATGG AATGGTCAGA 
TACATAGCAT TTGACTTCCA TAAAGAGTGC AGTCGGATGA GATGGGATCG ACTTCAGATT 
TTACTGGATC AACTAGCAGA ACAGCAGGAT GAGTTTAGTT ATTTTCTAGT TGATTCGGAC 
GGTAAAATCG TGACTCAGCA GGAAGGAACA TTCCGCAGTA ACTGTATGGA CTGCCTGGAT 
AGGACAAATG TGATTCAGAG CTTGTTAGCA CGCCGATCTC TTCAAGCCCA GCTTCAGAGG 
CTAGGTGTTT TGCATGTTGG ACAGAGAATT GAGGAGCAAG CAGACTTTGA GAAAATCTAC 
AAAAATGCAT GGGCTGATAA TGCTAATGCT TGTGCCAAAC AGTATGCTGG AACTGGTGCC 
TTAAAGACAG ATTACACAAG AACTGGGAAG AGGACTCAGT GGGGCTTAAT AATGGATGGC 
TGGAACTCAT TAATACGTTA TTACAAAAAT AACTTTTCTG ATGGATTTAG ACAAGATGCT 
ATTGATCTCT TCCTTGGAAA TTATTCAGTG GATGAAGTAG AATCTGCTAG TCCTCTACAT 
GTCAAGAAAG ATTGGAAGTT CTTAGCTTTG CCTATTATTA TGGTGGTTGC TTTCTCCATG 
TGCATCATTT GTTTGCTAAT GGCTGGTGAT ACATGGACTG AGACACTGGC CTACGTGCTT 
TTCTGGGGAA CTGCAAGCTT TGGAACTTTT GCCATCATCC TTTACAACGG CAAGGATTTT 
GTAGATGCAC CCAAGCTGGT CCAGAAAGAG AAGATGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Nucleic acids research42(14)8928-38(2014 Aug)
Loughran G,Chou MY,Ivanov IP,Jungreis I,Kellis M,Kiran AM,Baranov PV,Atkins JF

The Sac1 phosphoinositide phosphatase regulates Golgi membrane morphology and mitotic spindle organization in mammals.
Molecular biology of the cell19(7)3080-96(2008 Jul)
Liu Y,Boukhelifa M,Tribble E,Morin-Kensicki E,Uetrecht A,Bear JE,Bankaitis VA

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SACM1L cDNA ORF clone, Gallus gallus(Chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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