SVIL cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SVIL
Entrez Gene ID	420475
Full Name	supervillin
General protein information	Preferred Names supervillin Names supervillin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015282099.1	XP_015137585.1	supervillin isoform X11
XM_015282107.1	XP_015137593.1	supervillin isoform X15
XM_015282110.1	XP_015137596.1	supervillin isoform X18
XM_025148341.1	XP_025004109.1	supervillin isoform X9
XM_025148347.1	XP_025004115.1	supervillin isoform X12
XM_025148349.1	XP_025004117.1	supervillin isoform X9
XM_025148356.1	XP_025004124.1	supervillin isoform X20
XM_015282111.2	XP_015137597.1	supervillin isoform X21
XM_015282097.1	XP_015137583.1	supervillin isoform X9
XM_015282109.1	XP_015137595.1	supervillin isoform X17
XM_015282100.1	XP_015137586.1	supervillin isoform X12
XM_025148339.1	XP_025004107.1	supervillin isoform X7
XM_025148352.1	XP_025004120.1	supervillin isoform X16
XM_004939197.2	XP_004939254.1	supervillin isoform X1
XM_015282094.1	XP_015137580.1	supervillin isoform X5
XM_015282095.1	XP_015137581.1	supervillin isoform X7
XM_015282098.1	XP_015137584.1	supervillin isoform X10
XM_015282101.1	XP_015137587.1	supervillin isoform X12
XM_015282104.1	XP_015137590.1	supervillin isoform X12
XM_015282106.1	XP_015137592.1	supervillin isoform X14
XM_015282111.1	XP_015137597.1	supervillin isoform X19
XM_015282091.2	XP_015137577.1	supervillin isoform X1
XM_025148337.1	XP_025004105.1	supervillin isoform X3
XM_025148342.1	XP_025004110.1	supervillin isoform X9
XM_025148343.1	XP_025004111.1	supervillin isoform X9
XM_025148345.1	XP_025004113.1	supervillin isoform X10
XM_025148346.1	XP_025004114.1	supervillin isoform X11
XM_025148355.1	XP_025004123.1	supervillin isoform X19
XM_025148357.1	XP_025004125.1	supervillin isoform X22
XM_015282108.1	XP_015137594.1	supervillin isoform X16
XM_015282112.1	XP_015137598.1	supervillin isoform X20
XM_015282091.1	XP_015137577.1	supervillin isoform X2
XM_015282093.1	XP_015137579.1	supervillin isoform X4
XM_015282096.1	XP_015137582.1	supervillin isoform X8
XM_015282102.1	XP_015137588.1	supervillin isoform X12
XM_015282105.1	XP_015137591.1	supervillin isoform X13
XM_025148336.1	XP_025004104.1	supervillin isoform X2
XM_025148338.1	XP_025004106.1	supervillin isoform X4
XM_015282095.2	XP_015137581.1	supervillin isoform X6
XM_025148340.1	XP_025004108.1	supervillin isoform X8
XM_025148353.1	XP_025004121.1	supervillin isoform X17
XM_025148354.1	XP_025004122.1	supervillin isoform X18
XM_015282092.1	XP_015137578.1	supervillin isoform X3
XM_004939199.2	XP_004939256.1	supervillin isoform X6
XM_015282103.1	XP_015137589.1	supervillin isoform X12
XM_015282113.1	XP_015137599.1	supervillin isoform X21
XM_004939199.3	XP_004939256.1	supervillin isoform X5
XM_025148344.1	XP_025004112.1	supervillin isoform X9
XM_025148348.1	XP_025004116.1	supervillin isoform X13
XM_025148350.1	XP_025004118.1	supervillin isoform X14
XM_025148351.1	XP_025004119.1	supervillin isoform X15

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SVIL	NP_776615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007181	XP_565017.3
Danio rerio (zebrafish)	svilb	XP_003197996.2
Xenopus tropicalis (tropical clawed frog)	svil	NP_001090765.1
Pan troglodytes (chimpanzee)	SVIL	XP_001136112.1
Canis lupus familiaris (dog)	SVIL	XP_005617106.1
Mus musculus (house mouse)	Svil	NP_694793.1
Rattus norvegicus (Norway rat)	Svil	NP_001101886.2
Gallus gallus (chicken)	SVIL	XP_004939254.1
	SVIL	XP_001083894.2
Danio rerio (zebrafish)	svila	NP_001030338.2
Homo sapiens (human)	SVIL	NP_068506.2
Drosophila melanogaster (fruit fly)	CG33232	NP_995968.1

Gene Ontology

The following SVIL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SVIL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa146736	XM_025148350.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa146737	XM_025148351.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa146738	XM_025148352.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa146739	XM_025148353.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa146740	XM_025148354.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa146741	XM_025148355.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa146743	XM_025148357.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa31980	XM_015282107.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa31981	XM_015282108.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa31982	XM_015282109.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa31983	XM_015282110.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa146726	XM_025148336.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146727	XM_025148337.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146728	XM_025148338.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146729	XM_025148339.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146730	XM_025148340.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146731	XM_025148343.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146731	XM_025148342.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146731	XM_025148341.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146731	XM_025148344.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146731	XM_025148349.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146732	XM_025148345.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146733	XM_025148346.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146734	XM_025148347.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146735	XM_025148348.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa146742	XM_025148356.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa31968	XM_015282091.2 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31969	XM_015282092.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31970	XM_015282093.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31971	XM_015282094.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31972	XM_015282095.2 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31973	XM_015282096.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31974	XM_015282097.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31975	XM_015282098.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31976	XM_015282099.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31977	XM_015282100.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31977	XM_015282101.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31977	XM_015282102.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31977	XM_015282103.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31977	XM_015282104.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31978	XM_015282105.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31979	XM_015282106.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31984	XM_015282111.2 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31985	XM_015282112.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31986	XM_015282113.1 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa04062	XM_004939197.2 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa04064	XM_004939199.3 Latest version!	Gallus gallus supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OGa31984	XM_015282111.1	Gallus gallus supervillin (SVIL), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31968	XM_015282091.1	Gallus gallus supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa31972	XM_015282095.1	Gallus gallus supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa04064	XM_004939199.2	Gallus gallus supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SVIL ( XM_025148350.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004118.1 Gallus gallus supervillin (SVIL), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OGa146736
Clone ID Related Accession (Same CDS sequence)	XM_025148350.1
Accession Version	XM_025148350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGAGGATAG CAGATAATCA GGAGAGTCAA ATGACTATAG AAGAAAGAAA ACACCTCATC 
ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA GGTAAAGGCG CAGCAAATGA CTCCACGCAG 
TTCACCGTAG CTGGTCGAAT GGTAAAAAAA GGCCTGGCTT CTCCAAGTGC ACTAACACCA 
GTAGCATCCC CTTATGGTAA CAGGCAGAAG TCTACAACTC CGGTCACAAA GCCCTTGGAA 
GAAATTGAAG CTAGGCCTGA TATGCAATTG GAATCAGATA TGAAGCTTGA CAAGCTGGAG 
TCATTCTTGG GAAGGCTGAA TAACAAAGTC GCTGGAATGC AAGAGACAGT GTTGACAGTT 
ACAGGAAAAT CTGTGAAAGA GGTGATGAAG CTGGATGATG ATGAAACATT TTCCAAATTT 
TACCGCCGTG TGGATTTCCC TTCCTCCTCT GTGCCTTTGG ACCTTGATGA GGACTTTGAT 
GCCATCTTTG ACCCTTATGC ACCAAAGTTA ATATCCTCTG TAGCAGAGCA CAAACGTGCA 
GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC AGAAACCCCC TGAAAATGCT GGCAGCAAGA 
GAAGATATTC TTCATGAGTA CACTGAACAA AGACTGAATG TTGCCTTCAT GGAGTCAAAG 
CGAATGAAAG TAGAAAAAAT GTCTGCAAAC TCAAATTTCT CAGAAGTAGC ACTTGCTGGT 
TTGGCAAGCA AGGAGAACTT CAGCAACGTT AGTCTGCGGA GTGTTAACCT AACAGAGCAA 
AATTCTAACA ACAGTGCTGT GCCATACAAG AAGTTAATGC TGCTGCAAAT TAAAGGAAGA 
AGACATGTTC AAACAAGATT AGTTGAACCA AGGGCCTCAT CACTGAACAG TGGAGACTGT 
TTCCTATTGT TAACTCCACA TCTCTGTTTC TTATGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAAGCAA AGGCTTCAGA ACTTGCAACT TTGATTCAGA CAAAGAGGGA ACTTGGCTGT 
AGAGCTTCTT ATATACAGAC TATAGAAGAA GGAATAAACA CCCATACCCA TGCAGCCAAA 
GACTTCTGGA AACTCCTTGG TGGCCAAGCA AATTACCAGT CTGCTGGAAG ACCTGAAGAG 
GATGAAATGT ATGAAGCTGC CATAATAGAA ACTAATTGCA TTTACCGTCT TGTAGAGGAT 
AAACTCATTC CTGAAGATGA CTACTGGGGA AAGATGCCAA AATGTACCCT GCTACAACCA 
AAAGAGGTGC TCGTATTTGA TTTTGGCAGT GAAGTGTATG TCTGGCATGG AAAAGAAGTT 
ACTTTAGCAC AAAGAAAAGT GGCATTCCAG CTGGCAAAAC ACTTATGGAA TGGCACCTTC 
GACTACTCAA ATTGTGACAT AAATCCTCTG GACCCAGGAG AATGCAATCC CCTCATACCC 
AGAAAGGGAC AAGGACGTCC AGACTGGGCT GTGTTTGGCA GGCTCACAGA ACATAATGAG 
ACCATACTGT TCAAAGAAAA ATTCCTTGAT TGGACAGAGC TGAAGAAACT CAATGAGAAG 
AATTCTAGTG AATCCCTTCA CCAGAAGGAA GAATCCAAAT CTGATTCTAA ACCATATGAT 
GTCATGCTAA TGGTACCTGT GCCCCAAACT GCTGTAGGCA CGGTTCTGGA TGGAATGAAT 
ATTGGCCGGG GCTATGGACT TGTAGAAGGA GAAGATGGGA GGCAGTTTGA AATTATCACT 
GCATCTGTGG ATGTCTGGCA CATCCTGGAG TTTGATTATA GTAGATTGCC TAAACAAAGC 
ATAGGACAGT TCCATGAGGG AGACACATAC GTGGTGAAGT GGAAGTACAT GGTGAGCACT 
ACAGTTGGAA GCAGACAAAA AGGAGAGCAA CAAGTAAGAG CTGTTGGAAA AGAGAAATGT 
GTGTATTTCT TTTGGCAAGG AAGGCACTCC ACAGTGAGTG AGAAAGGGAC ATCGGCACTG 
ATGACAGTGG AACTTGATGA GGAGAGAGGA GCACAGGTAC AAGTGCTTCA AGGGAAAGAA 
CTACCATGCT TCCTGCAGTG CTTCCAAGGA GGGATGATTG TGCATGCTGG AAGAAGGGAA 
GAGGAAGAAG AAAATGCACA AAGTGACTGG AGACTATATT GCGTGCGAGG AGAAGTTCCC 
AATGAAGGAA ACTTACTTGA AGTAGCATGT CACTGCAGCA GCCTGCGTTC CCGGACATCC 
ATGATTGTCC TCAATATAAA TAAAGCTCTT ATCTACCTGT GGCATGGCTG CAAAGCACAA 
TCTCACACCA AGGATGTAGG AAGAACAGCA GCCAATAAAA TAAAAGAACA ATGTCCGCTG 
GAAGCAGGGC TGCACAGCAG CAGCAAGGTG ACAATACACG AATGTGATGA AGGGTCAGAG 
CCTTTGGGAT TCTGGGATGC ATTAGGAAGG CGAGATCGAA AGGCCTATGA TTGCATGTTG 
CAAGATCCGG GAAAGTTTAA TTTCACCCCC CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA 
GAATTCTCAG CCACTGAGTA CGTTTACCCT TCAAGAGACC CTACTGTCAT CAATTCTATG 
CCCTTCTTGC AAGAGGATCT TTACACTGCC CCACAGCCAG CACTGTTCCT TGTTGACAAT 
CACCATGAAG TGTACCTGTG GCAAGGCTGG TGGCCTGTGG AAAACAAAAT CACTGGATCA 
GCTCGAATCA GGTGGGCTAC AGACAGGAAA TGCGCCATGG AGACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG TCCTATCTCA TTCATGCCGG CCTAGAGCCT 
CTGACCTTCA CTAATATGTT CCCAAGTTGG GAACACAGGG AAGACATTGC AGAGATCACA 
GAAATGGATG CTGACGTTTC TAATCAGATA ATCCTTGTAG AAGATGTGCT GGCCAAGCTG 
TGTAAAACTG TGTATCCCCT AGCTGATCTC TTAGCCAGGC CTTTACCTGA GGGAGTTGAT 
CCTCTGAAGC TTGAAATTTA TCTTTCAGAT GAGGACTTTG AGGTTGCATT AGAGATGACA 
AGAGAAGAGT ACAATGCGCT GCCATCTTGG AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA 
CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004118.1
CDS	17..5965
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148350.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148350.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGAGGATAG CAGATAATCA GGAGAGTCAA ATGACTATAG AAGAAAGAAA ACACCTCATC 
ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA GGTAAAGGCG CAGCAAATGA CTCCACGCAG 
TTCACCGTAG CTGGTCGAAT GGTAAAAAAA GGCCTGGCTT CTCCAAGTGC ACTAACACCA 
GTAGCATCCC CTTATGGTAA CAGGCAGAAG TCTACAACTC CGGTCACAAA GCCCTTGGAA 
GAAATTGAAG CTAGGCCTGA TATGCAATTG GAATCAGATA TGAAGCTTGA CAAGCTGGAG 
TCATTCTTGG GAAGGCTGAA TAACAAAGTC GCTGGAATGC AAGAGACAGT GTTGACAGTT 
ACAGGAAAAT CTGTGAAAGA GGTGATGAAG CTGGATGATG ATGAAACATT TTCCAAATTT 
TACCGCCGTG TGGATTTCCC TTCCTCCTCT GTGCCTTTGG ACCTTGATGA GGACTTTGAT 
GCCATCTTTG ACCCTTATGC ACCAAAGTTA ATATCCTCTG TAGCAGAGCA CAAACGTGCA 
GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC AGAAACCCCC TGAAAATGCT GGCAGCAAGA 
GAAGATATTC TTCATGAGTA CACTGAACAA AGACTGAATG TTGCCTTCAT GGAGTCAAAG 
CGAATGAAAG TAGAAAAAAT GTCTGCAAAC TCAAATTTCT CAGAAGTAGC ACTTGCTGGT 
TTGGCAAGCA AGGAGAACTT CAGCAACGTT AGTCTGCGGA GTGTTAACCT AACAGAGCAA 
AATTCTAACA ACAGTGCTGT GCCATACAAG AAGTTAATGC TGCTGCAAAT TAAAGGAAGA 
AGACATGTTC AAACAAGATT AGTTGAACCA AGGGCCTCAT CACTGAACAG TGGAGACTGT 
TTCCTATTGT TAACTCCACA TCTCTGTTTC TTATGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAAGCAA AGGCTTCAGA ACTTGCAACT TTGATTCAGA CAAAGAGGGA ACTTGGCTGT 
AGAGCTTCTT ATATACAGAC TATAGAAGAA GGAATAAACA CCCATACCCA TGCAGCCAAA 
GACTTCTGGA AACTCCTTGG TGGCCAAGCA AATTACCAGT CTGCTGGAAG ACCTGAAGAG 
GATGAAATGT ATGAAGCTGC CATAATAGAA ACTAATTGCA TTTACCGTCT TGTAGAGGAT 
AAACTCATTC CTGAAGATGA CTACTGGGGA AAGATGCCAA AATGTACCCT GCTACAACCA 
AAAGAGGTGC TCGTATTTGA TTTTGGCAGT GAAGTGTATG TCTGGCATGG AAAAGAAGTT 
ACTTTAGCAC AAAGAAAAGT GGCATTCCAG CTGGCAAAAC ACTTATGGAA TGGCACCTTC 
GACTACTCAA ATTGTGACAT AAATCCTCTG GACCCAGGAG AATGCAATCC CCTCATACCC 
AGAAAGGGAC AAGGACGTCC AGACTGGGCT GTGTTTGGCA GGCTCACAGA ACATAATGAG 
ACCATACTGT TCAAAGAAAA ATTCCTTGAT TGGACAGAGC TGAAGAAACT CAATGAGAAG 
AATTCTAGTG AATCCCTTCA CCAGAAGGAA GAATCCAAAT CTGATTCTAA ACCATATGAT 
GTCATGCTAA TGGTACCTGT GCCCCAAACT GCTGTAGGCA CGGTTCTGGA TGGAATGAAT 
ATTGGCCGGG GCTATGGACT TGTAGAAGGA GAAGATGGGA GGCAGTTTGA AATTATCACT 
GCATCTGTGG ATGTCTGGCA CATCCTGGAG TTTGATTATA GTAGATTGCC TAAACAAAGC 
ATAGGACAGT TCCATGAGGG AGACACATAC GTGGTGAAGT GGAAGTACAT GGTGAGCACT 
ACAGTTGGAA GCAGACAAAA AGGAGAGCAA CAAGTAAGAG CTGTTGGAAA AGAGAAATGT 
GTGTATTTCT TTTGGCAAGG AAGGCACTCC ACAGTGAGTG AGAAAGGGAC ATCGGCACTG 
ATGACAGTGG AACTTGATGA GGAGAGAGGA GCACAGGTAC AAGTGCTTCA AGGGAAAGAA 
CTACCATGCT TCCTGCAGTG CTTCCAAGGA GGGATGATTG TGCATGCTGG AAGAAGGGAA 
GAGGAAGAAG AAAATGCACA AAGTGACTGG AGACTATATT GCGTGCGAGG AGAAGTTCCC 
AATGAAGGAA ACTTACTTGA AGTAGCATGT CACTGCAGCA GCCTGCGTTC CCGGACATCC 
ATGATTGTCC TCAATATAAA TAAAGCTCTT ATCTACCTGT GGCATGGCTG CAAAGCACAA 
TCTCACACCA AGGATGTAGG AAGAACAGCA GCCAATAAAA TAAAAGAACA ATGTCCGCTG 
GAAGCAGGGC TGCACAGCAG CAGCAAGGTG ACAATACACG AATGTGATGA AGGGTCAGAG 
CCTTTGGGAT TCTGGGATGC ATTAGGAAGG CGAGATCGAA AGGCCTATGA TTGCATGTTG 
CAAGATCCGG GAAAGTTTAA TTTCACCCCC CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA 
GAATTCTCAG CCACTGAGTA CGTTTACCCT TCAAGAGACC CTACTGTCAT CAATTCTATG 
CCCTTCTTGC AAGAGGATCT TTACACTGCC CCACAGCCAG CACTGTTCCT TGTTGACAAT 
CACCATGAAG TGTACCTGTG GCAAGGCTGG TGGCCTGTGG AAAACAAAAT CACTGGATCA 
GCTCGAATCA GGTGGGCTAC AGACAGGAAA TGCGCCATGG AGACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG TCCTATCTCA TTCATGCCGG CCTAGAGCCT 
CTGACCTTCA CTAATATGTT CCCAAGTTGG GAACACAGGG AAGACATTGC AGAGATCACA 
GAAATGGATG CTGACGTTTC TAATCAGATA ATCCTTGTAG AAGATGTGCT GGCCAAGCTG 
TGTAAAACTG TGTATCCCCT AGCTGATCTC TTAGCCAGGC CTTTACCTGA GGGAGTTGAT 
CCTCTGAAGC TTGAAATTTA TCTTTCAGAT GAGGACTTTG AGGTTGCATT AGAGATGACA 
AGAGAAGAGT ACAATGCGCT GCCATCTTGG AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA 
CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148351.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004119.1 Gallus gallus supervillin (SVIL), transcript variant X19, mRNA.

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CloneID	OGa146737
Clone ID Related Accession (Same CDS sequence)	XM_025148351.1
Accession Version	XM_025148351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAATGTC CGCTGGAAGC AGGGCTGCAC AGCAGCAGCA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004119.1
CDS	23..5968
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQNVRWKQGCTAAAR

Target ORF information:

RefSeq Version	XM_025148351.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148351.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAATGTC CGCTGGAAGC AGGGCTGCAC AGCAGCAGCA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148352.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004120.1 Gallus gallus supervillin (SVIL), transcript variant X20, mRNA.

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CloneID	OGa146738
Clone ID Related Accession (Same CDS sequence)	XM_025148352.1
Accession Version	XM_025148352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACTGAAAC TACCCCTGCT 
TCACGTTCTG TGAATACCCA CACAGTAGTG GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG 
AGCACTGTAC AACCTACCAG CTTGCAGGCA TCAGCACACC AAAAAATACT AGCTAAAGAG 
GAGATAAGAG CAGCCAAAGA GGCGATGGGG CAAGATCAAT CCGATGAACC AGATTCTTCC 
ACATTAAGCT TGGCAGAAAA GATGGCTTTG TTTAACAAAC TGTCTCAGCC AGTATCCAGG 
GCTATATCCA CAAGGAGTCG GGGAGATATT AGGCACAGAA GGATGAATAC TCGTTACCAG 
ACCCAGCCAG TCACTCTTGG AGAAGTTGAG CAGATGCAAT GGAAAAAGAG TAAACCGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGAGGATAGC AGATAATCAG GAGAGTCAAA TGACTATAGA AGAAAGAAAA 
CACCTCATCA CAGCTCGAGA AGAAGCCTGG AAGTCCAGAG GTAAAGGCGC AGCAAATGAC 
TCCACGCAGT TCACCGTAGC TGGTCGAATG GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA 
CTAACACCAG TAGCATCCCC TTATGGTAAC AGGCAGAAGT CTACAACTCC GGTCACAAAG 
CCCTTGGAAG AAATTGAAGC TAGGCCTGAT ATGCAATTGG AATCAGATAT GAAGCTTGAC 
AAGCTGGAGT CATTCTTGGG AAGGCTGAAT AACAAAGTCG CTGGAATGCA AGAGACAGTG 
TTGACAGTTA CAGGAAAATC TGTGAAAGAG GTGATGAAGC TGGATGATGA TGAAACATTT 
TCCAAATTTT ACCGCCGTGT GGATTTCCCT TCCTCCTCTG TGCCTTTGGA CCTTGATGAG 
GACTTTGATG CCATCTTTGA CCCTTATGCA CCAAAGTTAA TATCCTCTGT AGCAGAGCAC 
AAACGTGCAG TGAGGCCTAT GCGCAGGGTT CAGTCCTCCA GAAACCCCCT GAAAATGCTG 
GCAGCAAGAG AAGATATTCT TCATGAGTAC ACTGAACAAA GACTGAATGT TGCCTTCATG 
GAGTCAAAGC GAATGAAAGT AGAAAAAATG TCTGCAAACT CAAATTTCTC AGAAGTAGCA 
CTTGCTGGTT TGGCAAGCAA GGAGAACTTC AGCAACGTTA GTCTGCGGAG TGTTAACCTA 
ACAGAGCAAA ATTCTAACAA CAGTGCTGTG CCATACAAGA AGTTAATGCT GCTGCAAATT 
AAAGGAAGAA GACATGTTCA AACAAGATTA GTTGAACCAA GGGCCTCATC ACTGAACAGT 
GGAGACTGTT TCCTATTGTT AACTCCACAT CTCTGTTTCT TATGGGTAGG AGAGTTTGCA 
AATGTCATAG AAAAAGCAAA GGCTTCAGAA CTTGCAACTT TGATTCAGAC AAAGAGGGAA 
CTTGGCTGTA GAGCTTCTTA TATACAGACT ATAGAAGAAG GAATAAACAC CCATACCCAT 
GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT GGCCAAGCAA ATTACCAGTC TGCTGGAAGA 
CCTGAAGAGG ATGAAATGTA TGAAGCTGCC ATAATAGAAA CTAATTGCAT TTACCGTCTT 
GTAGAGGATA AACTCATTCC TGAAGATGAC TACTGGGGAA AGATGCCAAA ATGTACCCTG 
CTACAACCAA AAGAGGTGCT CGTATTTGAT TTTGGCAGTG AAGTGTATGT CTGGCATGGA 
AAAGAAGTTA CTTTAGCACA AAGAAAAGTG GCATTCCAGC TGGCAAAACA CTTATGGAAT 
GGCACCTTCG ACTACTCAAA TTGTGACATA AATCCTCTGG ACCCAGGAGA ATGCAATCCC 
CTCATACCCA GAAAGGGACA AGGACGTCCA GACTGGGCTG TGTTTGGCAG GCTCACAGAA 
CATAATGAGA CCATACTGTT CAAAGAAAAA TTCCTTGATT GGACAGAGCT GAAGAAACTC 
AATGAGAAGA ATTCTAGTGA ATCCCTTCAC CAGAAGGAAG AATCCAAATC TGATTCTAAA 
CCATATGATG TCATGCTAAT GGTACCTGTG CCCCAAACTG CTGTAGGCAC GGTTCTGGAT 
GGAATGAATA TTGGCCGGGG CTATGGACTT GTAGAAGGAG AAGATGGGAG GCAGTTTGAA 
ATTATCACTG CATCTGTGGA TGTCTGGCAC ATCCTGGAGT TTGATTATAG TAGATTGCCT 
AAACAAAGCA TAGGACAGTT CCATGAGGGA GACACATACG TGGTGAAGTG GAAGTACATG 
GTGAGCACTA CAGTTGGAAG CAGACAAAAA GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA 
GAGAAATGTG TGTATTTCTT TTGGCAAGGA AGGCACTCCA CAGTGAGTGA GAAAGGGACA 
TCGGCACTGA TGACAGTGGA ACTTGATGAG GAGAGAGGAG CACAGGTACA AGTGCTTCAA 
GGGAAAGAAC TACCATGCTT CCTGCAGTGC TTCCAAGGAG GGATGATTGT GCATGCTGGA 
AGAAGGGAAG AGGAAGAAGA AAATGCACAA AGTGACTGGA GACTATATTG CGTGCGAGGA 
GAAGTTCCCA ATGAAGGAAA CTTACTTGAA GTAGCATGTC ACTGCAGCAG CCTGCGTTCC 
CGGACATCCA TGATTGTCCT CAATATAAAT AAAGCTCTTA TCTACCTGTG GCATGGCTGC 
AAAGCACAAT CTCACACCAA GGATGTAGGA AGAACAGCAG CCAATAAAAT AAAAGAACAA 
TGTCCGCTGG AAGCAGGGCT GCACAGCAGC AGCAAGGTGA CAATACACGA ATGTGATGAA 
GGGTCAGAGC CTTTGGGATT CTGGGATGCA TTAGGAAGGC GAGATCGAAA GGCCTATGAT 
TGCATGTTGC AAGATCCGGG AAAGTTTAAT TTCACCCCCC GCCTCTTCAG CCTCAGTAGT 
TCTTCGGGAG AATTCTCAGC CACTGAGTAC GTTTACCCTT CAAGAGACCC TACTGTCATC 
AATTCTATGC CCTTCTTGCA AGAGGATCTT TACACTGCCC CACAGCCAGC ACTGTTCCTT 
GTTGACAATC ACCATGAAGT GTACCTGTGG CAAGGCTGGT GGCCTGTGGA AAACAAAATC 
ACTGGATCAG CTCGAATCAG GTGGGCTACA GACAGGAAAT GCGCCATGGA GACCGTGCTC 
CAGTACTGCA AAGGAAAAAA TGTAAAGAAG CCCCCAAAGT CCTATCTCAT TCATGCCGGC 
CTAGAGCCTC TGACCTTCAC TAATATGTTC CCAAGTTGGG AACACAGGGA AGACATTGCA 
GAGATCACAG AAATGGATGC TGACGTTTCT AATCAGATAA TCCTTGTAGA AGATGTGCTG 
GCCAAGCTGT GTAAAACTGT GTATCCCCTA GCTGATCTCT TAGCCAGGCC TTTACCTGAG 
GGAGTTGATC CTCTGAAGCT TGAAATTTAT CTTTCAGATG AGGACTTTGA GGTTGCATTA 
GAGATGACAA GAGAAGAGTA CAATGCGCTG CCATCTTGGA AGCAGGTTAA TCTGAAAAAG 
GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004120.1
CDS	17..5794
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148352.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148352.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACTGAAAC TACCCCTGCT 
TCACGTTCTG TGAATACCCA CACAGTAGTG GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG 
AGCACTGTAC AACCTACCAG CTTGCAGGCA TCAGCACACC AAAAAATACT AGCTAAAGAG 
GAGATAAGAG CAGCCAAAGA GGCGATGGGG CAAGATCAAT CCGATGAACC AGATTCTTCC 
ACATTAAGCT TGGCAGAAAA GATGGCTTTG TTTAACAAAC TGTCTCAGCC AGTATCCAGG 
GCTATATCCA CAAGGAGTCG GGGAGATATT AGGCACAGAA GGATGAATAC TCGTTACCAG 
ACCCAGCCAG TCACTCTTGG AGAAGTTGAG CAGATGCAAT GGAAAAAGAG TAAACCGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGAGGATAGC AGATAATCAG GAGAGTCAAA TGACTATAGA AGAAAGAAAA 
CACCTCATCA CAGCTCGAGA AGAAGCCTGG AAGTCCAGAG GTAAAGGCGC AGCAAATGAC 
TCCACGCAGT TCACCGTAGC TGGTCGAATG GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA 
CTAACACCAG TAGCATCCCC TTATGGTAAC AGGCAGAAGT CTACAACTCC GGTCACAAAG 
CCCTTGGAAG AAATTGAAGC TAGGCCTGAT ATGCAATTGG AATCAGATAT GAAGCTTGAC 
AAGCTGGAGT CATTCTTGGG AAGGCTGAAT AACAAAGTCG CTGGAATGCA AGAGACAGTG 
TTGACAGTTA CAGGAAAATC TGTGAAAGAG GTGATGAAGC TGGATGATGA TGAAACATTT 
TCCAAATTTT ACCGCCGTGT GGATTTCCCT TCCTCCTCTG TGCCTTTGGA CCTTGATGAG 
GACTTTGATG CCATCTTTGA CCCTTATGCA CCAAAGTTAA TATCCTCTGT AGCAGAGCAC 
AAACGTGCAG TGAGGCCTAT GCGCAGGGTT CAGTCCTCCA GAAACCCCCT GAAAATGCTG 
GCAGCAAGAG AAGATATTCT TCATGAGTAC ACTGAACAAA GACTGAATGT TGCCTTCATG 
GAGTCAAAGC GAATGAAAGT AGAAAAAATG TCTGCAAACT CAAATTTCTC AGAAGTAGCA 
CTTGCTGGTT TGGCAAGCAA GGAGAACTTC AGCAACGTTA GTCTGCGGAG TGTTAACCTA 
ACAGAGCAAA ATTCTAACAA CAGTGCTGTG CCATACAAGA AGTTAATGCT GCTGCAAATT 
AAAGGAAGAA GACATGTTCA AACAAGATTA GTTGAACCAA GGGCCTCATC ACTGAACAGT 
GGAGACTGTT TCCTATTGTT AACTCCACAT CTCTGTTTCT TATGGGTAGG AGAGTTTGCA 
AATGTCATAG AAAAAGCAAA GGCTTCAGAA CTTGCAACTT TGATTCAGAC AAAGAGGGAA 
CTTGGCTGTA GAGCTTCTTA TATACAGACT ATAGAAGAAG GAATAAACAC CCATACCCAT 
GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT GGCCAAGCAA ATTACCAGTC TGCTGGAAGA 
CCTGAAGAGG ATGAAATGTA TGAAGCTGCC ATAATAGAAA CTAATTGCAT TTACCGTCTT 
GTAGAGGATA AACTCATTCC TGAAGATGAC TACTGGGGAA AGATGCCAAA ATGTACCCTG 
CTACAACCAA AAGAGGTGCT CGTATTTGAT TTTGGCAGTG AAGTGTATGT CTGGCATGGA 
AAAGAAGTTA CTTTAGCACA AAGAAAAGTG GCATTCCAGC TGGCAAAACA CTTATGGAAT 
GGCACCTTCG ACTACTCAAA TTGTGACATA AATCCTCTGG ACCCAGGAGA ATGCAATCCC 
CTCATACCCA GAAAGGGACA AGGACGTCCA GACTGGGCTG TGTTTGGCAG GCTCACAGAA 
CATAATGAGA CCATACTGTT CAAAGAAAAA TTCCTTGATT GGACAGAGCT GAAGAAACTC 
AATGAGAAGA ATTCTAGTGA ATCCCTTCAC CAGAAGGAAG AATCCAAATC TGATTCTAAA 
CCATATGATG TCATGCTAAT GGTACCTGTG CCCCAAACTG CTGTAGGCAC GGTTCTGGAT 
GGAATGAATA TTGGCCGGGG CTATGGACTT GTAGAAGGAG AAGATGGGAG GCAGTTTGAA 
ATTATCACTG CATCTGTGGA TGTCTGGCAC ATCCTGGAGT TTGATTATAG TAGATTGCCT 
AAACAAAGCA TAGGACAGTT CCATGAGGGA GACACATACG TGGTGAAGTG GAAGTACATG 
GTGAGCACTA CAGTTGGAAG CAGACAAAAA GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA 
GAGAAATGTG TGTATTTCTT TTGGCAAGGA AGGCACTCCA CAGTGAGTGA GAAAGGGACA 
TCGGCACTGA TGACAGTGGA ACTTGATGAG GAGAGAGGAG CACAGGTACA AGTGCTTCAA 
GGGAAAGAAC TACCATGCTT CCTGCAGTGC TTCCAAGGAG GGATGATTGT GCATGCTGGA 
AGAAGGGAAG AGGAAGAAGA AAATGCACAA AGTGACTGGA GACTATATTG CGTGCGAGGA 
GAAGTTCCCA ATGAAGGAAA CTTACTTGAA GTAGCATGTC ACTGCAGCAG CCTGCGTTCC 
CGGACATCCA TGATTGTCCT CAATATAAAT AAAGCTCTTA TCTACCTGTG GCATGGCTGC 
AAAGCACAAT CTCACACCAA GGATGTAGGA AGAACAGCAG CCAATAAAAT AAAAGAACAA 
TGTCCGCTGG AAGCAGGGCT GCACAGCAGC AGCAAGGTGA CAATACACGA ATGTGATGAA 
GGGTCAGAGC CTTTGGGATT CTGGGATGCA TTAGGAAGGC GAGATCGAAA GGCCTATGAT 
TGCATGTTGC AAGATCCGGG AAAGTTTAAT TTCACCCCCC GCCTCTTCAG CCTCAGTAGT 
TCTTCGGGAG AATTCTCAGC CACTGAGTAC GTTTACCCTT CAAGAGACCC TACTGTCATC 
AATTCTATGC CCTTCTTGCA AGAGGATCTT TACACTGCCC CACAGCCAGC ACTGTTCCTT 
GTTGACAATC ACCATGAAGT GTACCTGTGG CAAGGCTGGT GGCCTGTGGA AAACAAAATC 
ACTGGATCAG CTCGAATCAG GTGGGCTACA GACAGGAAAT GCGCCATGGA GACCGTGCTC 
CAGTACTGCA AAGGAAAAAA TGTAAAGAAG CCCCCAAAGT CCTATCTCAT TCATGCCGGC 
CTAGAGCCTC TGACCTTCAC TAATATGTTC CCAAGTTGGG AACACAGGGA AGACATTGCA 
GAGATCACAG AAATGGATGC TGACGTTTCT AATCAGATAA TCCTTGTAGA AGATGTGCTG 
GCCAAGCTGT GTAAAACTGT GTATCCCCTA GCTGATCTCT TAGCCAGGCC TTTACCTGAG 
GGAGTTGATC CTCTGAAGCT TGAAATTTAT CTTTCAGATG AGGACTTTGA GGTTGCATTA 
GAGATGACAA GAGAAGAGTA CAATGCGCTG CCATCTTGGA AGCAGGTTAA TCTGAAAAAG 
GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148353.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004121.1 Gallus gallus supervillin (SVIL), transcript variant X21, mRNA.

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CloneID	OGa146739
Clone ID Related Accession (Same CDS sequence)	XM_025148353.1
Accession Version	XM_025148353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGATG 
CAATGGAAAA AGAGTAAACC GGTGCAAAAC GAAAGTGGAA AACTAACTCC CCTGTCAAGT 
ACAGTTTCAA CATCAGTTTC AGCAATGGCT TCTGCCCTTT CTGCACTGTT TGCTGGAGAC 
ATGCATGCGA AGTCACGTAG TGATGGAAGT GTGAGCGCTG CCACAAAAGA TGAGTTGAGA 
TTCCATGCTT CAGCAGAAAG CTCAGACTCA TCAGGAAAAC GCACACAGAA GTCAGAACAG 
TGGCAGCCCT CAATGGAAGT GCTGGAAAGC AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA 
GGAAAGAGGT TTCTAGCACA TGAGGCTAAT GAAATCAGAA AGTACAGCTC CTTTGAGGAT 
GAAACCACAC ATCCTGTTCT TGAAAAAACA GGAGACTACC ATAAAGAGGC AGCCTACAGC 
TTCCTGAGGA AGGGCAGCAT GGAACTGTTT AGTTCACAAC CACTTTTTCA GCCTCTTGAA 
AGGAAGGAAA TTGATACCAT CATGCAAGAC CACGTGGAGC CTACCTCTGA ACTCACCAGC 
ACACCAGCAA CGAGGACACT TTCCCAGACT ACAGCTGCAG CATCCTGTAG GCTACAGGAG 
CTGTCTGAAC AACTGGAGGG AAAGTTCTAC AAGAATTCCT GTGAGATGTT TTCTGCTGGA 
GAGAACAAAA TACAGACAAC TGAGGATGCC ACCGATAGCT CCAGCAAAAC GATGTCAATT 
AAAGAAAGGT TGGCATTGCT GAAGAAAAGT GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT 
AAAAAGCAGG AATATGCAAA AGTGTCTGCC ACTGATCGTA GCGCACAGAT GCAAGAAGTT 
GAGCAGCTGT TGAAAAAGGA ACCTGTATAT GCTTCTACTT ACTCTCCTGT TATACCTGCT 
GTCCATAAAT TTCATCCTTT TCTACCTATT AATCAGAGGA TAGCAGATAA TCAGGAGAGT 
CAAATGACTA TAGAAGAAAG AAAACACCTC ATCACAGCTC GAGAAGAAGC CTGGAAGTCC 
AGAGGTAAAG GCGCAGCAAA TGACTCCACG CAGTTCACCG TAGCTGGTCG AATGGTAAAA 
AAAGGCCTGG CTTCTCCAAG TGCACTAACA CCAGTAGCAT CCCCTTATGG TAACAGGCAG 
AAGTCTACAA CTCCGGTCAC AAAGCCCTTG GAAGAAATTG AAGCTAGGCC TGATATGCAA 
TTGGAATCAG ATATGAAGCT TGACAAGCTG GAGTCATTCT TGGGAAGGCT GAATAACAAA 
GTCGCTGGAA TGCAAGAGAC AGTGTTGACA GTTACAGGAA AATCTGTGAA AGAGGTGATG 
AAGCTGGATG ATGATGAAAC ATTTTCCAAA TTTTACCGCC GTGTGGATTT CCCTTCCTCC 
TCTGTGCCTT TGGACCTTGA TGAGGACTTT GATGCCATCT TTGACCCTTA TGCACCAAAG 
TTAATATCCT CTGTAGCAGA GCACAAACGT GCAGTGAGGC CTATGCGCAG GGTTCAGTCC 
TCCAGAAACC CCCTGAAAAT GCTGGCAGCA AGAGAAGATA TTCTTCATGA GTACACTGAA 
CAAAGACTGA ATGTTGCCTT CATGGAGTCA AAGCGAATGA AAGTAGAAAA AATGTCTGCA 
AACTCAAATT TCTCAGAAGT AGCACTTGCT GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC 
GTTAGTCTGC GGAGTGTTAA CCTAACAGAG CAAAATTCTA ACAACAGTGC TGTGCCATAC 
AAGAAGTTAA TGCTGCTGCA AATTAAAGGA AGAAGACATG TTCAAACAAG ATTAGTTGAA 
CCAAGGGCCT CATCACTGAA CAGTGGAGAC TGTTTCCTAT TGTTAACTCC ACATCTCTGT 
TTCTTATGGG TAGGAGAGTT TGCAAATGTC ATAGAAAAAG CAAAGGCTTC AGAACTTGCA 
ACTTTGATTC AGACAAAGAG GGAACTTGGC TGTAGAGCTT CTTATATACA GACTATAGAA 
GAAGGAATAA ACACCCATAC CCATGCAGCC AAAGACTTCT GGAAACTCCT TGGTGGCCAA 
GCAAATTACC AGTCTGCTGG AAGACCTGAA GAGGATGAAA TGTATGAAGC TGCCATAATA 
GAAACTAATT GCATTTACCG TCTTGTAGAG GATAAACTCA TTCCTGAAGA TGACTACTGG 
GGAAAGATGC CAAAATGTAC CCTGCTACAA CCAAAAGAGG TGCTCGTATT TGATTTTGGC 
AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA GTTACTTTAG CACAAAGAAA AGTGGCATTC 
CAGCTGGCAA AACACTTATG GAATGGCACC TTCGACTACT CAAATTGTGA CATAAATCCT 
CTGGACCCAG GAGAATGCAA TCCCCTCATA CCCAGAAAGG GACAAGGACG TCCAGACTGG 
GCTGTGTTTG GCAGGCTCAC AGAACATAAT GAGACCATAC TGTTCAAAGA AAAATTCCTT 
GATTGGACAG AGCTGAAGAA ACTCAATGAG AAGAATTCTA GTGAATCCCT TCACCAGAAG 
GAAGAATCCA AATCTGATTC TAAACCATAT GATGTCATGC TAATGGTACC TGTGCCCCAA 
ACTGCTGTAG GCACGGTTCT GGATGGAATG AATATTGGCC GGGGCTATGG ACTTGTAGAA 
GGAGAAGATG GGAGGCAGTT TGAAATTATC ACTGCATCTG TGGATGTCTG GCACATCCTG 
GAGTTTGATT ATAGTAGATT GCCTAAACAA AGCATAGGAC AGTTCCATGA GGGAGACACA 
TACGTGGTGA AGTGGAAGTA CATGGTGAGC ACTACAGTTG GAAGCAGACA AAAAGGAGAG 
CAACAAGTAA GAGCTGTTGG AAAAGAGAAA TGTGTGTATT TCTTTTGGCA AGGAAGGCAC 
TCCACAGTGA GTGAGAAAGG GACATCGGCA CTGATGACAG TGGAACTTGA TGAGGAGAGA 
GGAGCACAGG TACAAGTGCT TCAAGGGAAA GAACTACCAT GCTTCCTGCA GTGCTTCCAA 
GGAGGGATGA TTGTGCATGC TGGAAGAAGG GAAGAGGAAG AAGAAAATGC ACAAAGTGAC 
TGGAGACTAT ATTGCGTGCG AGGAGAAGTT CCCAATGAAG GAAACTTACT TGAAGTAGCA 
TGTCACTGCA GCAGCCTGCG TTCCCGGACA TCCATGATTG TCCTCAATAT AAATAAAGCT 
CTTATCTACC TGTGGCATGG CTGCAAAGCA CAATCTCACA CCAAGGATGT AGGAAGAACA 
GCAGCCAATA AAATAAAAGA ACAATGTCCG CTGGAAGCAG GGCTGCACAG CAGCAGCAAG 
GTGACAATAC ACGAATGTGA TGAAGGGTCA GAGCCTTTGG GATTCTGGGA TGCATTAGGA 
AGGCGAGATC GAAAGGCCTA TGATTGCATG TTGCAAGATC CGGGAAAGTT TAATTTCACC 
CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG GGAGAATTCT CAGCCACTGA GTACGTTTAC 
CCTTCAAGAG ACCCTACTGT CATCAATTCT ATGCCCTTCT TGCAAGAGGA TCTTTACACT 
GCCCCACAGC CAGCACTGTT CCTTGTTGAC AATCACCATG AAGTGTACCT GTGGCAAGGC 
TGGTGGCCTG TGGAAAACAA AATCACTGGA TCAGCTCGAA TCAGGTGGGC TACAGACAGG 
AAATGCGCCA TGGAGACCGT GCTCCAGTAC TGCAAAGGAA AAAATGTAAA GAAGCCCCCA 
AAGTCCTATC TCATTCATGC CGGCCTAGAG CCTCTGACCT TCACTAATAT GTTCCCAAGT 
TGGGAACACA GGGAAGACAT TGCAGAGATC ACAGAAATGG ATGCTGACGT TTCTAATCAG 
ATAATCCTTG TAGAAGATGT GCTGGCCAAG CTGTGTAAAA CTGTGTATCC CCTAGCTGAT 
CTCTTAGCCA GGCCTTTACC TGAGGGAGTT GATCCTCTGA AGCTTGAAAT TTATCTTTCA 
GATGAGGACT TTGAGGTTGC ATTAGAGATG ACAAGAGAAG AGTACAATGC GCTGCCATCT 
TGGAAGCAGG TTAATCTGAA AAAGGCAAAA GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004121.1
CDS	17..5698
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148353.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148353.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGATG 
CAATGGAAAA AGAGTAAACC GGTGCAAAAC GAAAGTGGAA AACTAACTCC CCTGTCAAGT 
ACAGTTTCAA CATCAGTTTC AGCAATGGCT TCTGCCCTTT CTGCACTGTT TGCTGGAGAC 
ATGCATGCGA AGTCACGTAG TGATGGAAGT GTGAGCGCTG CCACAAAAGA TGAGTTGAGA 
TTCCATGCTT CAGCAGAAAG CTCAGACTCA TCAGGAAAAC GCACACAGAA GTCAGAACAG 
TGGCAGCCCT CAATGGAAGT GCTGGAAAGC AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA 
GGAAAGAGGT TTCTAGCACA TGAGGCTAAT GAAATCAGAA AGTACAGCTC CTTTGAGGAT 
GAAACCACAC ATCCTGTTCT TGAAAAAACA GGAGACTACC ATAAAGAGGC AGCCTACAGC 
TTCCTGAGGA AGGGCAGCAT GGAACTGTTT AGTTCACAAC CACTTTTTCA GCCTCTTGAA 
AGGAAGGAAA TTGATACCAT CATGCAAGAC CACGTGGAGC CTACCTCTGA ACTCACCAGC 
ACACCAGCAA CGAGGACACT TTCCCAGACT ACAGCTGCAG CATCCTGTAG GCTACAGGAG 
CTGTCTGAAC AACTGGAGGG AAAGTTCTAC AAGAATTCCT GTGAGATGTT TTCTGCTGGA 
GAGAACAAAA TACAGACAAC TGAGGATGCC ACCGATAGCT CCAGCAAAAC GATGTCAATT 
AAAGAAAGGT TGGCATTGCT GAAGAAAAGT GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT 
AAAAAGCAGG AATATGCAAA AGTGTCTGCC ACTGATCGTA GCGCACAGAT GCAAGAAGTT 
GAGCAGCTGT TGAAAAAGGA ACCTGTATAT GCTTCTACTT ACTCTCCTGT TATACCTGCT 
GTCCATAAAT TTCATCCTTT TCTACCTATT AATCAGAGGA TAGCAGATAA TCAGGAGAGT 
CAAATGACTA TAGAAGAAAG AAAACACCTC ATCACAGCTC GAGAAGAAGC CTGGAAGTCC 
AGAGGTAAAG GCGCAGCAAA TGACTCCACG CAGTTCACCG TAGCTGGTCG AATGGTAAAA 
AAAGGCCTGG CTTCTCCAAG TGCACTAACA CCAGTAGCAT CCCCTTATGG TAACAGGCAG 
AAGTCTACAA CTCCGGTCAC AAAGCCCTTG GAAGAAATTG AAGCTAGGCC TGATATGCAA 
TTGGAATCAG ATATGAAGCT TGACAAGCTG GAGTCATTCT TGGGAAGGCT GAATAACAAA 
GTCGCTGGAA TGCAAGAGAC AGTGTTGACA GTTACAGGAA AATCTGTGAA AGAGGTGATG 
AAGCTGGATG ATGATGAAAC ATTTTCCAAA TTTTACCGCC GTGTGGATTT CCCTTCCTCC 
TCTGTGCCTT TGGACCTTGA TGAGGACTTT GATGCCATCT TTGACCCTTA TGCACCAAAG 
TTAATATCCT CTGTAGCAGA GCACAAACGT GCAGTGAGGC CTATGCGCAG GGTTCAGTCC 
TCCAGAAACC CCCTGAAAAT GCTGGCAGCA AGAGAAGATA TTCTTCATGA GTACACTGAA 
CAAAGACTGA ATGTTGCCTT CATGGAGTCA AAGCGAATGA AAGTAGAAAA AATGTCTGCA 
AACTCAAATT TCTCAGAAGT AGCACTTGCT GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC 
GTTAGTCTGC GGAGTGTTAA CCTAACAGAG CAAAATTCTA ACAACAGTGC TGTGCCATAC 
AAGAAGTTAA TGCTGCTGCA AATTAAAGGA AGAAGACATG TTCAAACAAG ATTAGTTGAA 
CCAAGGGCCT CATCACTGAA CAGTGGAGAC TGTTTCCTAT TGTTAACTCC ACATCTCTGT 
TTCTTATGGG TAGGAGAGTT TGCAAATGTC ATAGAAAAAG CAAAGGCTTC AGAACTTGCA 
ACTTTGATTC AGACAAAGAG GGAACTTGGC TGTAGAGCTT CTTATATACA GACTATAGAA 
GAAGGAATAA ACACCCATAC CCATGCAGCC AAAGACTTCT GGAAACTCCT TGGTGGCCAA 
GCAAATTACC AGTCTGCTGG AAGACCTGAA GAGGATGAAA TGTATGAAGC TGCCATAATA 
GAAACTAATT GCATTTACCG TCTTGTAGAG GATAAACTCA TTCCTGAAGA TGACTACTGG 
GGAAAGATGC CAAAATGTAC CCTGCTACAA CCAAAAGAGG TGCTCGTATT TGATTTTGGC 
AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA GTTACTTTAG CACAAAGAAA AGTGGCATTC 
CAGCTGGCAA AACACTTATG GAATGGCACC TTCGACTACT CAAATTGTGA CATAAATCCT 
CTGGACCCAG GAGAATGCAA TCCCCTCATA CCCAGAAAGG GACAAGGACG TCCAGACTGG 
GCTGTGTTTG GCAGGCTCAC AGAACATAAT GAGACCATAC TGTTCAAAGA AAAATTCCTT 
GATTGGACAG AGCTGAAGAA ACTCAATGAG AAGAATTCTA GTGAATCCCT TCACCAGAAG 
GAAGAATCCA AATCTGATTC TAAACCATAT GATGTCATGC TAATGGTACC TGTGCCCCAA 
ACTGCTGTAG GCACGGTTCT GGATGGAATG AATATTGGCC GGGGCTATGG ACTTGTAGAA 
GGAGAAGATG GGAGGCAGTT TGAAATTATC ACTGCATCTG TGGATGTCTG GCACATCCTG 
GAGTTTGATT ATAGTAGATT GCCTAAACAA AGCATAGGAC AGTTCCATGA GGGAGACACA 
TACGTGGTGA AGTGGAAGTA CATGGTGAGC ACTACAGTTG GAAGCAGACA AAAAGGAGAG 
CAACAAGTAA GAGCTGTTGG AAAAGAGAAA TGTGTGTATT TCTTTTGGCA AGGAAGGCAC 
TCCACAGTGA GTGAGAAAGG GACATCGGCA CTGATGACAG TGGAACTTGA TGAGGAGAGA 
GGAGCACAGG TACAAGTGCT TCAAGGGAAA GAACTACCAT GCTTCCTGCA GTGCTTCCAA 
GGAGGGATGA TTGTGCATGC TGGAAGAAGG GAAGAGGAAG AAGAAAATGC ACAAAGTGAC 
TGGAGACTAT ATTGCGTGCG AGGAGAAGTT CCCAATGAAG GAAACTTACT TGAAGTAGCA 
TGTCACTGCA GCAGCCTGCG TTCCCGGACA TCCATGATTG TCCTCAATAT AAATAAAGCT 
CTTATCTACC TGTGGCATGG CTGCAAAGCA CAATCTCACA CCAAGGATGT AGGAAGAACA 
GCAGCCAATA AAATAAAAGA ACAATGTCCG CTGGAAGCAG GGCTGCACAG CAGCAGCAAG 
GTGACAATAC ACGAATGTGA TGAAGGGTCA GAGCCTTTGG GATTCTGGGA TGCATTAGGA 
AGGCGAGATC GAAAGGCCTA TGATTGCATG TTGCAAGATC CGGGAAAGTT TAATTTCACC 
CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG GGAGAATTCT CAGCCACTGA GTACGTTTAC 
CCTTCAAGAG ACCCTACTGT CATCAATTCT ATGCCCTTCT TGCAAGAGGA TCTTTACACT 
GCCCCACAGC CAGCACTGTT CCTTGTTGAC AATCACCATG AAGTGTACCT GTGGCAAGGC 
TGGTGGCCTG TGGAAAACAA AATCACTGGA TCAGCTCGAA TCAGGTGGGC TACAGACAGG 
AAATGCGCCA TGGAGACCGT GCTCCAGTAC TGCAAAGGAA AAAATGTAAA GAAGCCCCCA 
AAGTCCTATC TCATTCATGC CGGCCTAGAG CCTCTGACCT TCACTAATAT GTTCCCAAGT 
TGGGAACACA GGGAAGACAT TGCAGAGATC ACAGAAATGG ATGCTGACGT TTCTAATCAG 
ATAATCCTTG TAGAAGATGT GCTGGCCAAG CTGTGTAAAA CTGTGTATCC CCTAGCTGAT 
CTCTTAGCCA GGCCTTTACC TGAGGGAGTT GATCCTCTGA AGCTTGAAAT TTATCTTTCA 
GATGAGGACT TTGAGGTTGC ATTAGAGATG ACAAGAGAAG AGTACAATGC GCTGCCATCT 
TGGAAGCAGG TTAATCTGAA AAAGGCAAAA GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148354.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004122.1 Gallus gallus supervillin (SVIL), transcript variant X22, mRNA.

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CloneID	OGa146740
Clone ID Related Accession (Same CDS sequence)	XM_025148354.1
Accession Version	XM_025148354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGAGGATAGC AGATAATCAG GAGAGTCAAA TGACTATAGA AGAAAGAAAA 
CACCTCATCA CAGCTCGAGA AGAAGCCTGG AAGTCCAGAG GTAAAGGCGC AGCAAATGAC 
TCCACGCAGT TCACCGTAGC TGGTCGAATG GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA 
CTAACACCAG TAGCATCCCC TTATGGTAAC AGGCAGAAGT CTACAACTCC GGTCACAAAG 
CCCTTGGAAG AAATTGAAGC TAGGCCTGAT ATGCAATTGG AATCAGATAT GAAGCTTGAC 
AAGCTGGAGT CATTCTTGGG AAGGCTGAAT AACAAAGTCG CTGGAATGCA AGAGACAGTG 
TTGACAGTTA CAGGAAAATC TGTGAAAGAG GTGATGAAGC TGGATGATGA TGAAACATTT 
TCCAAATTTT ACCGCCGTGT GGATTTCCCT TCCTCCTCTG TGCCTTTGGA CCTTGATGAG 
GACTTTGATG CCATCTTTGA CCCTTATGCA CCAAAGTTAA TATCCTCTGT AGCAGAGCAC 
AAACGTGCAG TGAGGCCTAT GCGCAGGGTT CAGTCCTCCA GAAACCCCCT GAAAATGCTG 
GCAGCAAGAG AAGATATTCT TCATGAGTAC ACTGAACAAA GACTGAATGT TGCCTTCATG 
GAGTCAAAGC GAATGAAAGT AGAAAAAATG TCTGCAAACT CAAATTTCTC AGAAGTAGCA 
CTTGCTGGTT TGGCAAGCAA GGAGAACTTC AGCAACGTTA GTCTGCGGAG TGTTAACCTA 
ACAGAGCAAA ATTCTAACAA CAGTGCTGTG CCATACAAGA AGTTAATGCT GCTGCAAATT 
AAAGGAAGAA GACATGTTCA AACAAGATTA GTTGAACCAA GGGCCTCATC ACTGAACAGT 
GGAGACTGTT TCCTATTGTT AACTCCACAT CTCTGTTTCT TATGGGTAGG AGAGTTTGCA 
AATGTCATAG AAAAAGCAAA GGCTTCAGAA CTTGCAACTT TGATTCAGAC AAAGAGGGAA 
CTTGGCTGTA GAGCTTCTTA TATACAGACT ATAGAAGAAG GAATAAACAC CCATACCCAT 
GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT GGCCAAGCAA ATTACCAGTC TGCTGGAAGA 
CCTGAAGAGG ATGAAATGTA TGAAGCTGCC ATAATAGAAA CTAATTGCAT TTACCGTCTT 
GTAGAGGATA AACTCATTCC TGAAGATGAC TACTGGGGAA AGATGCCAAA ATGTACCCTG 
CTACAACCAA AAGAGGTGCT CGTATTTGAT TTTGGCAGTG AAGTGTATGT CTGGCATGGA 
AAAGAAGTTA CTTTAGCACA AAGAAAAGTG GCATTCCAGC TGGCAAAACA CTTATGGAAT 
GGCACCTTCG ACTACTCAAA TTGTGACATA AATCCTCTGG ACCCAGGAGA ATGCAATCCC 
CTCATACCCA GAAAGGGACA AGGACGTCCA GACTGGGCTG TGTTTGGCAG GCTCACAGAA 
CATAATGAGA CCATACTGTT CAAAGAAAAA TTCCTTGATT GGACAGAGCT GAAGAAACTC 
AATGAGAAGA ATTCTAGTGA ATCCCTTCAC CAGAAGGAAG AATCCAAATC TGATTCTAAA 
CCATATGATG TCATGCTAAT GGTACCTGTG CCCCAAACTG CTGTAGGCAC GGTTCTGGAT 
GGAATGAATA TTGGCCGGGG CTATGGACTT GTAGAAGGAG AAGATGGGAG GCAGTTTGAA 
ATTATCACTG CATCTGTGGA TGTCTGGCAC ATCCTGGAGT TTGATTATAG TAGATTGCCT 
AAACAAAGCA TAGGACAGTT CCATGAGGGA GACACATACG TGGTGAAGTG GAAGTACATG 
GTGAGCACTA CAGTTGGAAG CAGACAAAAA GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA 
GAGAAATGTG TGTATTTCTT TTGGCAAGGA AGGCACTCCA CAGTGAGTGA GAAAGGGACA 
TCGGCACTGA TGACAGTGGA ACTTGATGAG GAGAGAGGAG CACAGGTACA AGTGCTTCAA 
GGGAAAGAAC TACCATGCTT CCTGCAGTGC TTCCAAGGAG GGATGATTGT GCATGCTGGA 
AGAAGGGAAG AGGAAGAAGA AAATGCACAA AGTGACTGGA GACTATATTG CGTGCGAGGA 
GAAGTTCCCA ATGAAGGAAA CTTACTTGAA GTAGCATGTC ACTGCAGCAG CCTGCGTTCC 
CGGACATCCA TGATTGTCCT CAATATAAAT AAAGCTCTTA TCTACCTGTG GCATGGCTGC 
AAAGCACAAT CTCACACCAA GGATGTAGGA AGAACAGCAG CCAATAAAAT AAAAGAACAA 
TGTCCGCTGG AAGCAGGGCT GCACAGCAGC AGCAAGGTGA CAATACACGA ATGTGATGAA 
GGGTCAGAGC CTTTGGGATT CTGGGATGCA TTAGGAAGGC GAGATCGAAA GGCCTATGAT 
TGCATGTTGC AAGATCCGGG AAAGTTTAAT TTCACCCCCC GCCTCTTCAG CCTCAGTAGT 
TCTTCGGGAG AATTCTCAGC CACTGAGTAC GTTTACCCTT CAAGAGACCC TACTGTCATC 
AATTCTATGC CCTTCTTGCA AGAGGATCTT TACACTGCCC CACAGCCAGC ACTGTTCCTT 
GTTGACAATC ACCATGAAGT GTACCTGTGG CAAGGCTGGT GGCCTGTGGA AAACAAAATC 
ACTGGATCAG CTCGAATCAG GTGGGCTACA GACAGGAAAT GCGCCATGGA GACCGTGCTC 
CAGTACTGCA AAGGAAAAAA TGTAAAGAAG CCCCCAAAGT CCTATCTCAT TCATGCCGGC 
CTAGAGCCTC TGACCTTCAC TAATATGTTC CCAAGTTGGG AACACAGGGA AGACATTGCA 
GAGATCACAG AAATGGATGC TGACGTTTCT AATCAGATAA TCCTTGTAGA AGATGTGCTG 
GCCAAGCTGT GTAAAACTGT GTATCCCCTA GCTGATCTCT TAGCCAGGCC TTTACCTGAG 
GGAGTTGATC CTCTGAAGCT TGAAATTTAT CTTTCAGATG AGGACTTTGA GGTTGCATTA 
GAGATGACAA GAGAAGAGTA CAATGCGCTG CCATCTTGGA AGCAGGTTAA TCTGAAAAAG 
GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004122.1
CDS	17..5674
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148354.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148354.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGAGGATAGC AGATAATCAG GAGAGTCAAA TGACTATAGA AGAAAGAAAA 
CACCTCATCA CAGCTCGAGA AGAAGCCTGG AAGTCCAGAG GTAAAGGCGC AGCAAATGAC 
TCCACGCAGT TCACCGTAGC TGGTCGAATG GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA 
CTAACACCAG TAGCATCCCC TTATGGTAAC AGGCAGAAGT CTACAACTCC GGTCACAAAG 
CCCTTGGAAG AAATTGAAGC TAGGCCTGAT ATGCAATTGG AATCAGATAT GAAGCTTGAC 
AAGCTGGAGT CATTCTTGGG AAGGCTGAAT AACAAAGTCG CTGGAATGCA AGAGACAGTG 
TTGACAGTTA CAGGAAAATC TGTGAAAGAG GTGATGAAGC TGGATGATGA TGAAACATTT 
TCCAAATTTT ACCGCCGTGT GGATTTCCCT TCCTCCTCTG TGCCTTTGGA CCTTGATGAG 
GACTTTGATG CCATCTTTGA CCCTTATGCA CCAAAGTTAA TATCCTCTGT AGCAGAGCAC 
AAACGTGCAG TGAGGCCTAT GCGCAGGGTT CAGTCCTCCA GAAACCCCCT GAAAATGCTG 
GCAGCAAGAG AAGATATTCT TCATGAGTAC ACTGAACAAA GACTGAATGT TGCCTTCATG 
GAGTCAAAGC GAATGAAAGT AGAAAAAATG TCTGCAAACT CAAATTTCTC AGAAGTAGCA 
CTTGCTGGTT TGGCAAGCAA GGAGAACTTC AGCAACGTTA GTCTGCGGAG TGTTAACCTA 
ACAGAGCAAA ATTCTAACAA CAGTGCTGTG CCATACAAGA AGTTAATGCT GCTGCAAATT 
AAAGGAAGAA GACATGTTCA AACAAGATTA GTTGAACCAA GGGCCTCATC ACTGAACAGT 
GGAGACTGTT TCCTATTGTT AACTCCACAT CTCTGTTTCT TATGGGTAGG AGAGTTTGCA 
AATGTCATAG AAAAAGCAAA GGCTTCAGAA CTTGCAACTT TGATTCAGAC AAAGAGGGAA 
CTTGGCTGTA GAGCTTCTTA TATACAGACT ATAGAAGAAG GAATAAACAC CCATACCCAT 
GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT GGCCAAGCAA ATTACCAGTC TGCTGGAAGA 
CCTGAAGAGG ATGAAATGTA TGAAGCTGCC ATAATAGAAA CTAATTGCAT TTACCGTCTT 
GTAGAGGATA AACTCATTCC TGAAGATGAC TACTGGGGAA AGATGCCAAA ATGTACCCTG 
CTACAACCAA AAGAGGTGCT CGTATTTGAT TTTGGCAGTG AAGTGTATGT CTGGCATGGA 
AAAGAAGTTA CTTTAGCACA AAGAAAAGTG GCATTCCAGC TGGCAAAACA CTTATGGAAT 
GGCACCTTCG ACTACTCAAA TTGTGACATA AATCCTCTGG ACCCAGGAGA ATGCAATCCC 
CTCATACCCA GAAAGGGACA AGGACGTCCA GACTGGGCTG TGTTTGGCAG GCTCACAGAA 
CATAATGAGA CCATACTGTT CAAAGAAAAA TTCCTTGATT GGACAGAGCT GAAGAAACTC 
AATGAGAAGA ATTCTAGTGA ATCCCTTCAC CAGAAGGAAG AATCCAAATC TGATTCTAAA 
CCATATGATG TCATGCTAAT GGTACCTGTG CCCCAAACTG CTGTAGGCAC GGTTCTGGAT 
GGAATGAATA TTGGCCGGGG CTATGGACTT GTAGAAGGAG AAGATGGGAG GCAGTTTGAA 
ATTATCACTG CATCTGTGGA TGTCTGGCAC ATCCTGGAGT TTGATTATAG TAGATTGCCT 
AAACAAAGCA TAGGACAGTT CCATGAGGGA GACACATACG TGGTGAAGTG GAAGTACATG 
GTGAGCACTA CAGTTGGAAG CAGACAAAAA GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA 
GAGAAATGTG TGTATTTCTT TTGGCAAGGA AGGCACTCCA CAGTGAGTGA GAAAGGGACA 
TCGGCACTGA TGACAGTGGA ACTTGATGAG GAGAGAGGAG CACAGGTACA AGTGCTTCAA 
GGGAAAGAAC TACCATGCTT CCTGCAGTGC TTCCAAGGAG GGATGATTGT GCATGCTGGA 
AGAAGGGAAG AGGAAGAAGA AAATGCACAA AGTGACTGGA GACTATATTG CGTGCGAGGA 
GAAGTTCCCA ATGAAGGAAA CTTACTTGAA GTAGCATGTC ACTGCAGCAG CCTGCGTTCC 
CGGACATCCA TGATTGTCCT CAATATAAAT AAAGCTCTTA TCTACCTGTG GCATGGCTGC 
AAAGCACAAT CTCACACCAA GGATGTAGGA AGAACAGCAG CCAATAAAAT AAAAGAACAA 
TGTCCGCTGG AAGCAGGGCT GCACAGCAGC AGCAAGGTGA CAATACACGA ATGTGATGAA 
GGGTCAGAGC CTTTGGGATT CTGGGATGCA TTAGGAAGGC GAGATCGAAA GGCCTATGAT 
TGCATGTTGC AAGATCCGGG AAAGTTTAAT TTCACCCCCC GCCTCTTCAG CCTCAGTAGT 
TCTTCGGGAG AATTCTCAGC CACTGAGTAC GTTTACCCTT CAAGAGACCC TACTGTCATC 
AATTCTATGC CCTTCTTGCA AGAGGATCTT TACACTGCCC CACAGCCAGC ACTGTTCCTT 
GTTGACAATC ACCATGAAGT GTACCTGTGG CAAGGCTGGT GGCCTGTGGA AAACAAAATC 
ACTGGATCAG CTCGAATCAG GTGGGCTACA GACAGGAAAT GCGCCATGGA GACCGTGCTC 
CAGTACTGCA AAGGAAAAAA TGTAAAGAAG CCCCCAAAGT CCTATCTCAT TCATGCCGGC 
CTAGAGCCTC TGACCTTCAC TAATATGTTC CCAAGTTGGG AACACAGGGA AGACATTGCA 
GAGATCACAG AAATGGATGC TGACGTTTCT AATCAGATAA TCCTTGTAGA AGATGTGCTG 
GCCAAGCTGT GTAAAACTGT GTATCCCCTA GCTGATCTCT TAGCCAGGCC TTTACCTGAG 
GGAGTTGATC CTCTGAAGCT TGAAATTTAT CTTTCAGATG AGGACTTTGA GGTTGCATTA 
GAGATGACAA GAGAAGAGTA CAATGCGCTG CCATCTTGGA AGCAGGTTAA TCTGAAAAAG 
GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148355.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004123.1 Gallus gallus supervillin (SVIL), transcript variant X23, mRNA.

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CloneID	OGa146741
Clone ID Related Accession (Same CDS sequence)	XM_025148355.1
Accession Version	XM_025148355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGAGGATA 
GCAGATAATC AGGAGAGTCA AATGACTATA GAAGAAAGAA AACACCTCAT CACAGCTCGA 
GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC GCAGCAAATG ACTCCACGCA GTTCACCGTA 
GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT TCTCCAAGTG CACTAACACC AGTAGCATCC 
CCTTATGGTA ACAGGCAGAA GTCTACAACT CCGGTCACAA AGCCCTTGGA AGAAATTGAA 
GCTAGGCCTG ATATGCAATT GGAATCAGAT ATGAAGCTTG ACAAGCTGGA GTCATTCTTG 
GGAAGGCTGA ATAACAAAGT CGCTGGAATG CAAGAGACAG TGTTGACAGT TACAGGAAAA 
TCTGTGAAAG AGGTGATGAA GCTGGATGAT GATGAAACAT TTTCCAAATT TTACCGCCGT 
GTGGATTTCC CTTCCTCCTC TGTGCCTTTG GACCTTGATG AGGACTTTGA TGCCATCTTT 
GACCCTTATG CACCAAAGTT AATATCCTCT GTAGCAGAGC ACAAACGTGC AGTGAGGCCT 
ATGCGCAGGG TTCAGTCCTC CAGAAACCCC CTGAAAATGC TGGCAGCAAG AGAAGATATT 
CTTCATGAGT ACACTGAACA AAGACTGAAT GTTGCCTTCA TGGAGTCAAA GCGAATGAAA 
GTAGAAAAAA TGTCTGCAAA CTCAAATTTC TCAGAAGTAG CACTTGCTGG TTTGGCAAGC 
AAGGAGAACT TCAGCAACGT TAGTCTGCGG AGTGTTAACC TAACAGAGCA AAATTCTAAC 
AACAGTGCTG TGCCATACAA GAAGTTAATG CTGCTGCAAA TTAAAGGAAG AAGACATGTT 
CAAACAAGAT TAGTTGAACC AAGGGCCTCA TCACTGAACA GTGGAGACTG TTTCCTATTG 
TTAACTCCAC ATCTCTGTTT CTTATGGGTA GGAGAGTTTG CAAATGTCAT AGAAAAAGCA 
AAGGCTTCAG AACTTGCAAC TTTGATTCAG ACAAAGAGGG AACTTGGCTG TAGAGCTTCT 
TATATACAGA CTATAGAAGA AGGAATAAAC ACCCATACCC ATGCAGCCAA AGACTTCTGG 
AAACTCCTTG GTGGCCAAGC AAATTACCAG TCTGCTGGAA GACCTGAAGA GGATGAAATG 
TATGAAGCTG CCATAATAGA AACTAATTGC ATTTACCGTC TTGTAGAGGA TAAACTCATT 
CCTGAAGATG ACTACTGGGG AAAGATGCCA AAATGTACCC TGCTACAACC AAAAGAGGTG 
CTCGTATTTG ATTTTGGCAG TGAAGTGTAT GTCTGGCATG GAAAAGAAGT TACTTTAGCA 
CAAAGAAAAG TGGCATTCCA GCTGGCAAAA CACTTATGGA ATGGCACCTT CGACTACTCA 
AATTGTGACA TAAATCCTCT GGACCCAGGA GAATGCAATC CCCTCATACC CAGAAAGGGA 
CAAGGACGTC CAGACTGGGC TGTGTTTGGC AGGCTCACAG AACATAATGA GACCATACTG 
TTCAAAGAAA AATTCCTTGA TTGGACAGAG CTGAAGAAAC TCAATGAGAA GAATTCTAGT 
GAATCCCTTC ACCAGAAGGA AGAATCCAAA TCTGATTCTA AACCATATGA TGTCATGCTA 
ATGGTACCTG TGCCCCAAAC TGCTGTAGGC ACGGTTCTGG ATGGAATGAA TATTGGCCGG 
GGCTATGGAC TTGTAGAAGG AGAAGATGGG AGGCAGTTTG AAATTATCAC TGCATCTGTG 
GATGTCTGGC ACATCCTGGA GTTTGATTAT AGTAGATTGC CTAAACAAAG CATAGGACAG 
TTCCATGAGG GAGACACATA CGTGGTGAAG TGGAAGTACA TGGTGAGCAC TACAGTTGGA 
AGCAGACAAA AAGGAGAGCA ACAAGTAAGA GCTGTTGGAA AAGAGAAATG TGTGTATTTC 
TTTTGGCAAG GAAGGCACTC CACAGTGAGT GAGAAAGGGA CATCGGCACT GATGACAGTG 
GAACTTGATG AGGAGAGAGG AGCACAGGTA CAAGTGCTTC AAGGGAAAGA ACTACCATGC 
TTCCTGCAGT GCTTCCAAGG AGGGATGATT GTGCATGCTG GAAGAAGGGA AGAGGAAGAA 
GAAAATGCAC AAAGTGACTG GAGACTATAT TGCGTGCGAG GAGAAGTTCC CAATGAAGGA 
AACTTACTTG AAGTAGCATG TCACTGCAGC AGCCTGCGTT CCCGGACATC CATGATTGTC 
CTCAATATAA ATAAAGCTCT TATCTACCTG TGGCATGGCT GCAAAGCACA ATCTCACACC 
AAGGATGTAG GAAGAACAGC AGCCAATAAA ATAAAAGAAC AATGTCCGCT GGAAGCAGGG 
CTGCACAGCA GCAGCAAGGT GACAATACAC GAATGTGATG AAGGGTCAGA GCCTTTGGGA 
TTCTGGGATG CATTAGGAAG GCGAGATCGA AAGGCCTATG ATTGCATGTT GCAAGATCCG 
GGAAAGTTTA ATTTCACCCC CCGCCTCTTC AGCCTCAGTA GTTCTTCGGG AGAATTCTCA 
GCCACTGAGT ACGTTTACCC TTCAAGAGAC CCTACTGTCA TCAATTCTAT GCCCTTCTTG 
CAAGAGGATC TTTACACTGC CCCACAGCCA GCACTGTTCC TTGTTGACAA TCACCATGAA 
GTGTACCTGT GGCAAGGCTG GTGGCCTGTG GAAAACAAAA TCACTGGATC AGCTCGAATC 
AGGTGGGCTA CAGACAGGAA ATGCGCCATG GAGACCGTGC TCCAGTACTG CAAAGGAAAA 
AATGTAAAGA AGCCCCCAAA GTCCTATCTC ATTCATGCCG GCCTAGAGCC TCTGACCTTC 
ACTAATATGT TCCCAAGTTG GGAACACAGG GAAGACATTG CAGAGATCAC AGAAATGGAT 
GCTGACGTTT CTAATCAGAT AATCCTTGTA GAAGATGTGC TGGCCAAGCT GTGTAAAACT 
GTGTATCCCC TAGCTGATCT CTTAGCCAGG CCTTTACCTG AGGGAGTTGA TCCTCTGAAG 
CTTGAAATTT ATCTTTCAGA TGAGGACTTT GAGGTTGCAT TAGAGATGAC AAGAGAAGAG 
TACAATGCGC TGCCATCTTG GAAGCAGGTT AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004123.1
CDS	17..5596
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148355.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148355.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGAGGATA 
GCAGATAATC AGGAGAGTCA AATGACTATA GAAGAAAGAA AACACCTCAT CACAGCTCGA 
GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC GCAGCAAATG ACTCCACGCA GTTCACCGTA 
GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT TCTCCAAGTG CACTAACACC AGTAGCATCC 
CCTTATGGTA ACAGGCAGAA GTCTACAACT CCGGTCACAA AGCCCTTGGA AGAAATTGAA 
GCTAGGCCTG ATATGCAATT GGAATCAGAT ATGAAGCTTG ACAAGCTGGA GTCATTCTTG 
GGAAGGCTGA ATAACAAAGT CGCTGGAATG CAAGAGACAG TGTTGACAGT TACAGGAAAA 
TCTGTGAAAG AGGTGATGAA GCTGGATGAT GATGAAACAT TTTCCAAATT TTACCGCCGT 
GTGGATTTCC CTTCCTCCTC TGTGCCTTTG GACCTTGATG AGGACTTTGA TGCCATCTTT 
GACCCTTATG CACCAAAGTT AATATCCTCT GTAGCAGAGC ACAAACGTGC AGTGAGGCCT 
ATGCGCAGGG TTCAGTCCTC CAGAAACCCC CTGAAAATGC TGGCAGCAAG AGAAGATATT 
CTTCATGAGT ACACTGAACA AAGACTGAAT GTTGCCTTCA TGGAGTCAAA GCGAATGAAA 
GTAGAAAAAA TGTCTGCAAA CTCAAATTTC TCAGAAGTAG CACTTGCTGG TTTGGCAAGC 
AAGGAGAACT TCAGCAACGT TAGTCTGCGG AGTGTTAACC TAACAGAGCA AAATTCTAAC 
AACAGTGCTG TGCCATACAA GAAGTTAATG CTGCTGCAAA TTAAAGGAAG AAGACATGTT 
CAAACAAGAT TAGTTGAACC AAGGGCCTCA TCACTGAACA GTGGAGACTG TTTCCTATTG 
TTAACTCCAC ATCTCTGTTT CTTATGGGTA GGAGAGTTTG CAAATGTCAT AGAAAAAGCA 
AAGGCTTCAG AACTTGCAAC TTTGATTCAG ACAAAGAGGG AACTTGGCTG TAGAGCTTCT 
TATATACAGA CTATAGAAGA AGGAATAAAC ACCCATACCC ATGCAGCCAA AGACTTCTGG 
AAACTCCTTG GTGGCCAAGC AAATTACCAG TCTGCTGGAA GACCTGAAGA GGATGAAATG 
TATGAAGCTG CCATAATAGA AACTAATTGC ATTTACCGTC TTGTAGAGGA TAAACTCATT 
CCTGAAGATG ACTACTGGGG AAAGATGCCA AAATGTACCC TGCTACAACC AAAAGAGGTG 
CTCGTATTTG ATTTTGGCAG TGAAGTGTAT GTCTGGCATG GAAAAGAAGT TACTTTAGCA 
CAAAGAAAAG TGGCATTCCA GCTGGCAAAA CACTTATGGA ATGGCACCTT CGACTACTCA 
AATTGTGACA TAAATCCTCT GGACCCAGGA GAATGCAATC CCCTCATACC CAGAAAGGGA 
CAAGGACGTC CAGACTGGGC TGTGTTTGGC AGGCTCACAG AACATAATGA GACCATACTG 
TTCAAAGAAA AATTCCTTGA TTGGACAGAG CTGAAGAAAC TCAATGAGAA GAATTCTAGT 
GAATCCCTTC ACCAGAAGGA AGAATCCAAA TCTGATTCTA AACCATATGA TGTCATGCTA 
ATGGTACCTG TGCCCCAAAC TGCTGTAGGC ACGGTTCTGG ATGGAATGAA TATTGGCCGG 
GGCTATGGAC TTGTAGAAGG AGAAGATGGG AGGCAGTTTG AAATTATCAC TGCATCTGTG 
GATGTCTGGC ACATCCTGGA GTTTGATTAT AGTAGATTGC CTAAACAAAG CATAGGACAG 
TTCCATGAGG GAGACACATA CGTGGTGAAG TGGAAGTACA TGGTGAGCAC TACAGTTGGA 
AGCAGACAAA AAGGAGAGCA ACAAGTAAGA GCTGTTGGAA AAGAGAAATG TGTGTATTTC 
TTTTGGCAAG GAAGGCACTC CACAGTGAGT GAGAAAGGGA CATCGGCACT GATGACAGTG 
GAACTTGATG AGGAGAGAGG AGCACAGGTA CAAGTGCTTC AAGGGAAAGA ACTACCATGC 
TTCCTGCAGT GCTTCCAAGG AGGGATGATT GTGCATGCTG GAAGAAGGGA AGAGGAAGAA 
GAAAATGCAC AAAGTGACTG GAGACTATAT TGCGTGCGAG GAGAAGTTCC CAATGAAGGA 
AACTTACTTG AAGTAGCATG TCACTGCAGC AGCCTGCGTT CCCGGACATC CATGATTGTC 
CTCAATATAA ATAAAGCTCT TATCTACCTG TGGCATGGCT GCAAAGCACA ATCTCACACC 
AAGGATGTAG GAAGAACAGC AGCCAATAAA ATAAAAGAAC AATGTCCGCT GGAAGCAGGG 
CTGCACAGCA GCAGCAAGGT GACAATACAC GAATGTGATG AAGGGTCAGA GCCTTTGGGA 
TTCTGGGATG CATTAGGAAG GCGAGATCGA AAGGCCTATG ATTGCATGTT GCAAGATCCG 
GGAAAGTTTA ATTTCACCCC CCGCCTCTTC AGCCTCAGTA GTTCTTCGGG AGAATTCTCA 
GCCACTGAGT ACGTTTACCC TTCAAGAGAC CCTACTGTCA TCAATTCTAT GCCCTTCTTG 
CAAGAGGATC TTTACACTGC CCCACAGCCA GCACTGTTCC TTGTTGACAA TCACCATGAA 
GTGTACCTGT GGCAAGGCTG GTGGCCTGTG GAAAACAAAA TCACTGGATC AGCTCGAATC 
AGGTGGGCTA CAGACAGGAA ATGCGCCATG GAGACCGTGC TCCAGTACTG CAAAGGAAAA 
AATGTAAAGA AGCCCCCAAA GTCCTATCTC ATTCATGCCG GCCTAGAGCC TCTGACCTTC 
ACTAATATGT TCCCAAGTTG GGAACACAGG GAAGACATTG CAGAGATCAC AGAAATGGAT 
GCTGACGTTT CTAATCAGAT AATCCTTGTA GAAGATGTGC TGGCCAAGCT GTGTAAAACT 
GTGTATCCCC TAGCTGATCT CTTAGCCAGG CCTTTACCTG AGGGAGTTGA TCCTCTGAAG 
CTTGAAATTT ATCTTTCAGA TGAGGACTTT GAGGTTGCAT TAGAGATGAC AAGAGAAGAG 
TACAATGCGC TGCCATCTTG GAAGCAGGTT AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148357.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004125.1 Gallus gallus supervillin (SVIL), transcript variant X26, mRNA.

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CloneID	OGa146743
Clone ID Related Accession (Same CDS sequence)	XM_025148357.1
Accession Version	XM_025148357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGATG 
CAATGGAAAA AGAGTAAACC GGTGCAAAAC GAAAGTGGAA AACTAACTCC CCTGTCAAGT 
ACAGTTTCAA CATCAGTTTC AGCAATGGCT TCTGCCCTTT CTGCACTGTT TGCTGGAGAC 
ATGCATGCGA AGTCACGTAG TGATGGAAGT GTGAGCGCTG CCACAAAAGA TGAGTTGAGA 
TTCCATGCTT CAGCAGAAAG CTCAGACTCA TCAGGAAAAC GCACACAGAA GTCAGAACAG 
TGGCAGCCCT CAATGGAAGT GCTGGAAAGC AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA 
GGAAAGAGGT TTCTAGCACA TGAGGCTAAT GAAATCAGAA AGTACAGCTC CTTTGAGGAT 
GAAACCACAC ATCCTGTTCT TGAAAAAACA GGAGACTACC ATAAAGAGGC AGCCTACAGC 
TTCCTGAGGA AGGGCAGCAT GGAACTGTTT AGTTCACAAC CACTTTTTCA GCCTCTTGAA 
AGGAAGGAAA TTGATACCAT CATGCAAGAC CACGTGGAGC CTACCTCTGA ACTCACCAGC 
ACACCAGCAA CGAGGACACT TTCCCAGACT ACAGCTGCAG CATCCTGTAG GCTACAGGAG 
CTGTCTGAAC AACTGGAGGG AAAGTTCTAC AAGAATTCCT GTGAGATGTT TTCTGCTGGA 
GAGAACAAAA TACAGACAAC TGAGGATGCC ACCGATAGCT CCAGCAAAAC GATGTCAATT 
AAAGAAAGGT TGGCATTGCT GAAGAAAAGT GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT 
AAAAAGCAGG AATATGCAAA AGTGTCTGCC ACTGATCGTA GCGCACAGAT GCAAGAAGTT 
GAGCAGCTGT TGAAAAAGCA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004125.1
CDS	17..5623
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148357.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148357.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGATG 
CAATGGAAAA AGAGTAAACC GGTGCAAAAC GAAAGTGGAA AACTAACTCC CCTGTCAAGT 
ACAGTTTCAA CATCAGTTTC AGCAATGGCT TCTGCCCTTT CTGCACTGTT TGCTGGAGAC 
ATGCATGCGA AGTCACGTAG TGATGGAAGT GTGAGCGCTG CCACAAAAGA TGAGTTGAGA 
TTCCATGCTT CAGCAGAAAG CTCAGACTCA TCAGGAAAAC GCACACAGAA GTCAGAACAG 
TGGCAGCCCT CAATGGAAGT GCTGGAAAGC AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA 
GGAAAGAGGT TTCTAGCACA TGAGGCTAAT GAAATCAGAA AGTACAGCTC CTTTGAGGAT 
GAAACCACAC ATCCTGTTCT TGAAAAAACA GGAGACTACC ATAAAGAGGC AGCCTACAGC 
TTCCTGAGGA AGGGCAGCAT GGAACTGTTT AGTTCACAAC CACTTTTTCA GCCTCTTGAA 
AGGAAGGAAA TTGATACCAT CATGCAAGAC CACGTGGAGC CTACCTCTGA ACTCACCAGC 
ACACCAGCAA CGAGGACACT TTCCCAGACT ACAGCTGCAG CATCCTGTAG GCTACAGGAG 
CTGTCTGAAC AACTGGAGGG AAAGTTCTAC AAGAATTCCT GTGAGATGTT TTCTGCTGGA 
GAGAACAAAA TACAGACAAC TGAGGATGCC ACCGATAGCT CCAGCAAAAC GATGTCAATT 
AAAGAAAGGT TGGCATTGCT GAAGAAAAGT GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT 
AAAAAGCAGG AATATGCAAA AGTGTCTGCC ACTGATCGTA GCGCACAGAT GCAAGAAGTT 
GAGCAGCTGT TGAAAAAGCA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282107.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137593.1 Gallus gallus supervillin (SVIL), transcript variant X19, mRNA.

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CloneID	OGa31980
Clone ID Related Accession (Same CDS sequence)	XM_015282107.1
Accession Version	XM_015282107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137593.1
CDS	16..5967
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282107.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282107.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282108.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137594.1 Gallus gallus supervillin (SVIL), transcript variant X20, mRNA.

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CloneID	OGa31981
Clone ID Related Accession (Same CDS sequence)	XM_015282108.1
Accession Version	XM_015282108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA 
GCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137594.1
CDS	20..5968
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQNVRWKQGCTAAAR

Target ORF information:

RefSeq Version	XM_015282108.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282108.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA 
GCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282109.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137595.1 Gallus gallus supervillin (SVIL), transcript variant X21, mRNA.

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CloneID	OGa31982
Clone ID Related Accession (Same CDS sequence)	XM_015282109.1
Accession Version	XM_015282109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACTGAAAC TACCCCTGCT 
TCACGTTCTG TGAATACCCA CACAGTAGTG GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG 
AGCACTGTAC AACCTACCAG CTTGCAGGCA TCAGCACACC AAAAAATACT AGCTAAAGAG 
GAGATAAGAG CAGCCAAAGA GGCGATGGGG CAAGATCAAT CCGATGAACC AGATTCTTCC 
ACATTAAGCT TGGCAGAAAA GATGGCTTTG TTTAACAAAC TGTCTCAGCC AGTATCCAGG 
GCTATATCCA CAAGGAGTCG GGGAGATATT AGGCACAGAA GGATGAATAC TCGTTACCAG 
ACCCAGCCAG TCACTCTTGG AGAAGTTGAG CAGATGCAAT GGAAAAAGAG TAAACCGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGCAGAGGAT AGCAGATAAT CAGGAGAGTC AAATGACTAT AGAAGAAAGA 
AAACACCTCA TCACAGCTCG AGAAGAAGCC TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT 
GACTCCACGC AGTTCACCGT AGCTGGTCGA ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT 
GCACTAACAC CAGTAGCATC CCCTTATGGT AACAGGCAGA AGTCTACAAC TCCGGTCACA 
AAGCCCTTGG AAGAAATTGA AGCTAGGCCT GATATGCAAT TGGAATCAGA TATGAAGCTT 
GACAAGCTGG AGTCATTCTT GGGAAGGCTG AATAACAAAG TCGCTGGAAT GCAAGAGACA 
GTGTTGACAG TTACAGGAAA ATCTGTGAAA GAGGTGATGA AGCTGGATGA TGATGAAACA 
TTTTCCAAAT TTTACCGCCG TGTGGATTTC CCTTCCTCCT CTGTGCCTTT GGACCTTGAT 
GAGGACTTTG ATGCCATCTT TGACCCTTAT GCACCAAAGT TAATATCCTC TGTAGCAGAG 
CACAAACGTG CAGTGAGGCC TATGCGCAGG GTTCAGTCCT CCAGAAACCC CCTGAAAATG 
CTGGCAGCAA GAGAAGATAT TCTTCATGAG TACACTGAAC AAAGACTGAA TGTTGCCTTC 
ATGGAGTCAA AGCGAATGAA AGTAGAAAAA ATGTCTGCAA ACTCAAATTT CTCAGAAGTA 
GCACTTGCTG GTTTGGCAAG CAAGGAGAAC TTCAGCAACG TTAGTCTGCG GAGTGTTAAC 
CTAACAGAGC AAAATTCTAA CAACAGTGCT GTGCCATACA AGAAGTTAAT GCTGCTGCAA 
ATTAAAGGAA GAAGACATGT TCAAACAAGA TTAGTTGAAC CAAGGGCCTC ATCACTGAAC 
AGTGGAGACT GTTTCCTATT GTTAACTCCA CATCTCTGTT TCTTATGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAAGC AAAGGCTTCA GAACTTGCAA CTTTGATTCA GACAAAGAGG 
GAACTTGGCT GTAGAGCTTC TTATATACAG ACTATAGAAG AAGGAATAAA CACCCATACC 
CATGCAGCCA AAGACTTCTG GAAACTCCTT GGTGGCCAAG CAAATTACCA GTCTGCTGGA 
AGACCTGAAG AGGATGAAAT GTATGAAGCT GCCATAATAG AAACTAATTG CATTTACCGT 
CTTGTAGAGG ATAAACTCAT TCCTGAAGAT GACTACTGGG GAAAGATGCC AAAATGTACC 
CTGCTACAAC CAAAAGAGGT GCTCGTATTT GATTTTGGCA GTGAAGTGTA TGTCTGGCAT 
GGAAAAGAAG TTACTTTAGC ACAAAGAAAA GTGGCATTCC AGCTGGCAAA ACACTTATGG 
AATGGCACCT TCGACTACTC AAATTGTGAC ATAAATCCTC TGGACCCAGG AGAATGCAAT 
CCCCTCATAC CCAGAAAGGG ACAAGGACGT CCAGACTGGG CTGTGTTTGG CAGGCTCACA 
GAACATAATG AGACCATACT GTTCAAAGAA AAATTCCTTG ATTGGACAGA GCTGAAGAAA 
CTCAATGAGA AGAATTCTAG TGAATCCCTT CACCAGAAGG AAGAATCCAA ATCTGATTCT 
AAACCATATG ATGTCATGCT AATGGTACCT GTGCCCCAAA CTGCTGTAGG CACGGTTCTG 
GATGGAATGA ATATTGGCCG GGGCTATGGA CTTGTAGAAG GAGAAGATGG GAGGCAGTTT 
GAAATTATCA CTGCATCTGT GGATGTCTGG CACATCCTGG AGTTTGATTA TAGTAGATTG 
CCTAAACAAA GCATAGGACA GTTCCATGAG GGAGACACAT ACGTGGTGAA GTGGAAGTAC 
ATGGTGAGCA CTACAGTTGG AAGCAGACAA AAAGGAGAGC AACAAGTAAG AGCTGTTGGA 
AAAGAGAAAT GTGTGTATTT CTTTTGGCAA GGAAGGCACT CCACAGTGAG TGAGAAAGGG 
ACATCGGCAC TGATGACAGT GGAACTTGAT GAGGAGAGAG GAGCACAGGT ACAAGTGCTT 
CAAGGGAAAG AACTACCATG CTTCCTGCAG TGCTTCCAAG GAGGGATGAT TGTGCATGCT 
GGAAGAAGGG AAGAGGAAGA AGAAAATGCA CAAAGTGACT GGAGACTATA TTGCGTGCGA 
GGAGAAGTTC CCAATGAAGG AAACTTACTT GAAGTAGCAT GTCACTGCAG CAGCCTGCGT 
TCCCGGACAT CCATGATTGT CCTCAATATA AATAAAGCTC TTATCTACCT GTGGCATGGC 
TGCAAAGCAC AATCTCACAC CAAGGATGTA GGAAGAACAG CAGCCAATAA AATAAAAGAA 
CAATGTCCGC TGGAAGCAGG GCTGCACAGC AGCAGCAAGG TGACAATACA CGAATGTGAT 
GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT GCATTAGGAA GGCGAGATCG AAAGGCCTAT 
GATTGCATGT TGCAAGATCC GGGAAAGTTT AATTTCACCC CCCGCCTCTT CAGCCTCAGT 
AGTTCTTCGG GAGAATTCTC AGCCACTGAG TACGTTTACC CTTCAAGAGA CCCTACTGTC 
ATCAATTCTA TGCCCTTCTT GCAAGAGGAT CTTTACACTG CCCCACAGCC AGCACTGTTC 
CTTGTTGACA ATCACCATGA AGTGTACCTG TGGCAAGGCT GGTGGCCTGT GGAAAACAAA 
ATCACTGGAT CAGCTCGAAT CAGGTGGGCT ACAGACAGGA AATGCGCCAT GGAGACCGTG 
CTCCAGTACT GCAAAGGAAA AAATGTAAAG AAGCCCCCAA AGTCCTATCT CATTCATGCC 
GGCCTAGAGC CTCTGACCTT CACTAATATG TTCCCAAGTT GGGAACACAG GGAAGACATT 
GCAGAGATCA CAGAAATGGA TGCTGACGTT TCTAATCAGA TAATCCTTGT AGAAGATGTG 
CTGGCCAAGC TGTGTAAAAC TGTGTATCCC CTAGCTGATC TCTTAGCCAG GCCTTTACCT 
GAGGGAGTTG ATCCTCTGAA GCTTGAAATT TATCTTTCAG ATGAGGACTT TGAGGTTGCA 
TTAGAGATGA CAAGAGAAGA GTACAATGCG CTGCCATCTT GGAAGCAGGT TAATCTGAAA 
AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137595.1
CDS	15..5795
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282109.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282109.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACTGAAAC TACCCCTGCT 
TCACGTTCTG TGAATACCCA CACAGTAGTG GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG 
AGCACTGTAC AACCTACCAG CTTGCAGGCA TCAGCACACC AAAAAATACT AGCTAAAGAG 
GAGATAAGAG CAGCCAAAGA GGCGATGGGG CAAGATCAAT CCGATGAACC AGATTCTTCC 
ACATTAAGCT TGGCAGAAAA GATGGCTTTG TTTAACAAAC TGTCTCAGCC AGTATCCAGG 
GCTATATCCA CAAGGAGTCG GGGAGATATT AGGCACAGAA GGATGAATAC TCGTTACCAG 
ACCCAGCCAG TCACTCTTGG AGAAGTTGAG CAGATGCAAT GGAAAAAGAG TAAACCGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGCAGAGGAT AGCAGATAAT CAGGAGAGTC AAATGACTAT AGAAGAAAGA 
AAACACCTCA TCACAGCTCG AGAAGAAGCC TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT 
GACTCCACGC AGTTCACCGT AGCTGGTCGA ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT 
GCACTAACAC CAGTAGCATC CCCTTATGGT AACAGGCAGA AGTCTACAAC TCCGGTCACA 
AAGCCCTTGG AAGAAATTGA AGCTAGGCCT GATATGCAAT TGGAATCAGA TATGAAGCTT 
GACAAGCTGG AGTCATTCTT GGGAAGGCTG AATAACAAAG TCGCTGGAAT GCAAGAGACA 
GTGTTGACAG TTACAGGAAA ATCTGTGAAA GAGGTGATGA AGCTGGATGA TGATGAAACA 
TTTTCCAAAT TTTACCGCCG TGTGGATTTC CCTTCCTCCT CTGTGCCTTT GGACCTTGAT 
GAGGACTTTG ATGCCATCTT TGACCCTTAT GCACCAAAGT TAATATCCTC TGTAGCAGAG 
CACAAACGTG CAGTGAGGCC TATGCGCAGG GTTCAGTCCT CCAGAAACCC CCTGAAAATG 
CTGGCAGCAA GAGAAGATAT TCTTCATGAG TACACTGAAC AAAGACTGAA TGTTGCCTTC 
ATGGAGTCAA AGCGAATGAA AGTAGAAAAA ATGTCTGCAA ACTCAAATTT CTCAGAAGTA 
GCACTTGCTG GTTTGGCAAG CAAGGAGAAC TTCAGCAACG TTAGTCTGCG GAGTGTTAAC 
CTAACAGAGC AAAATTCTAA CAACAGTGCT GTGCCATACA AGAAGTTAAT GCTGCTGCAA 
ATTAAAGGAA GAAGACATGT TCAAACAAGA TTAGTTGAAC CAAGGGCCTC ATCACTGAAC 
AGTGGAGACT GTTTCCTATT GTTAACTCCA CATCTCTGTT TCTTATGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAAGC AAAGGCTTCA GAACTTGCAA CTTTGATTCA GACAAAGAGG 
GAACTTGGCT GTAGAGCTTC TTATATACAG ACTATAGAAG AAGGAATAAA CACCCATACC 
CATGCAGCCA AAGACTTCTG GAAACTCCTT GGTGGCCAAG CAAATTACCA GTCTGCTGGA 
AGACCTGAAG AGGATGAAAT GTATGAAGCT GCCATAATAG AAACTAATTG CATTTACCGT 
CTTGTAGAGG ATAAACTCAT TCCTGAAGAT GACTACTGGG GAAAGATGCC AAAATGTACC 
CTGCTACAAC CAAAAGAGGT GCTCGTATTT GATTTTGGCA GTGAAGTGTA TGTCTGGCAT 
GGAAAAGAAG TTACTTTAGC ACAAAGAAAA GTGGCATTCC AGCTGGCAAA ACACTTATGG 
AATGGCACCT TCGACTACTC AAATTGTGAC ATAAATCCTC TGGACCCAGG AGAATGCAAT 
CCCCTCATAC CCAGAAAGGG ACAAGGACGT CCAGACTGGG CTGTGTTTGG CAGGCTCACA 
GAACATAATG AGACCATACT GTTCAAAGAA AAATTCCTTG ATTGGACAGA GCTGAAGAAA 
CTCAATGAGA AGAATTCTAG TGAATCCCTT CACCAGAAGG AAGAATCCAA ATCTGATTCT 
AAACCATATG ATGTCATGCT AATGGTACCT GTGCCCCAAA CTGCTGTAGG CACGGTTCTG 
GATGGAATGA ATATTGGCCG GGGCTATGGA CTTGTAGAAG GAGAAGATGG GAGGCAGTTT 
GAAATTATCA CTGCATCTGT GGATGTCTGG CACATCCTGG AGTTTGATTA TAGTAGATTG 
CCTAAACAAA GCATAGGACA GTTCCATGAG GGAGACACAT ACGTGGTGAA GTGGAAGTAC 
ATGGTGAGCA CTACAGTTGG AAGCAGACAA AAAGGAGAGC AACAAGTAAG AGCTGTTGGA 
AAAGAGAAAT GTGTGTATTT CTTTTGGCAA GGAAGGCACT CCACAGTGAG TGAGAAAGGG 
ACATCGGCAC TGATGACAGT GGAACTTGAT GAGGAGAGAG GAGCACAGGT ACAAGTGCTT 
CAAGGGAAAG AACTACCATG CTTCCTGCAG TGCTTCCAAG GAGGGATGAT TGTGCATGCT 
GGAAGAAGGG AAGAGGAAGA AGAAAATGCA CAAAGTGACT GGAGACTATA TTGCGTGCGA 
GGAGAAGTTC CCAATGAAGG AAACTTACTT GAAGTAGCAT GTCACTGCAG CAGCCTGCGT 
TCCCGGACAT CCATGATTGT CCTCAATATA AATAAAGCTC TTATCTACCT GTGGCATGGC 
TGCAAAGCAC AATCTCACAC CAAGGATGTA GGAAGAACAG CAGCCAATAA AATAAAAGAA 
CAATGTCCGC TGGAAGCAGG GCTGCACAGC AGCAGCAAGG TGACAATACA CGAATGTGAT 
GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT GCATTAGGAA GGCGAGATCG AAAGGCCTAT 
GATTGCATGT TGCAAGATCC GGGAAAGTTT AATTTCACCC CCCGCCTCTT CAGCCTCAGT 
AGTTCTTCGG GAGAATTCTC AGCCACTGAG TACGTTTACC CTTCAAGAGA CCCTACTGTC 
ATCAATTCTA TGCCCTTCTT GCAAGAGGAT CTTTACACTG CCCCACAGCC AGCACTGTTC 
CTTGTTGACA ATCACCATGA AGTGTACCTG TGGCAAGGCT GGTGGCCTGT GGAAAACAAA 
ATCACTGGAT CAGCTCGAAT CAGGTGGGCT ACAGACAGGA AATGCGCCAT GGAGACCGTG 
CTCCAGTACT GCAAAGGAAA AAATGTAAAG AAGCCCCCAA AGTCCTATCT CATTCATGCC 
GGCCTAGAGC CTCTGACCTT CACTAATATG TTCCCAAGTT GGGAACACAG GGAAGACATT 
GCAGAGATCA CAGAAATGGA TGCTGACGTT TCTAATCAGA TAATCCTTGT AGAAGATGTG 
CTGGCCAAGC TGTGTAAAAC TGTGTATCCC CTAGCTGATC TCTTAGCCAG GCCTTTACCT 
GAGGGAGTTG ATCCTCTGAA GCTTGAAATT TATCTTTCAG ATGAGGACTT TGAGGTTGCA 
TTAGAGATGA CAAGAGAAGA GTACAATGCG CTGCCATCTT GGAAGCAGGT TAATCTGAAA 
AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282110.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137596.1 Gallus gallus supervillin (SVIL), transcript variant X22, mRNA.

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CloneID	OGa31983
Clone ID Related Accession (Same CDS sequence)	XM_015282110.1
Accession Version	XM_015282110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGCAGAGGAT AGCAGATAAT CAGGAGAGTC AAATGACTAT AGAAGAAAGA 
AAACACCTCA TCACAGCTCG AGAAGAAGCC TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT 
GACTCCACGC AGTTCACCGT AGCTGGTCGA ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT 
GCACTAACAC CAGTAGCATC CCCTTATGGT AACAGGCAGA AGTCTACAAC TCCGGTCACA 
AAGCCCTTGG AAGAAATTGA AGCTAGGCCT GATATGCAAT TGGAATCAGA TATGAAGCTT 
GACAAGCTGG AGTCATTCTT GGGAAGGCTG AATAACAAAG TCGCTGGAAT GCAAGAGACA 
GTGTTGACAG TTACAGGAAA ATCTGTGAAA GAGGTGATGA AGCTGGATGA TGATGAAACA 
TTTTCCAAAT TTTACCGCCG TGTGGATTTC CCTTCCTCCT CTGTGCCTTT GGACCTTGAT 
GAGGACTTTG ATGCCATCTT TGACCCTTAT GCACCAAAGT TAATATCCTC TGTAGCAGAG 
CACAAACGTG CAGTGAGGCC TATGCGCAGG GTTCAGTCCT CCAGAAACCC CCTGAAAATG 
CTGGCAGCAA GAGAAGATAT TCTTCATGAG TACACTGAAC AAAGACTGAA TGTTGCCTTC 
ATGGAGTCAA AGCGAATGAA AGTAGAAAAA ATGTCTGCAA ACTCAAATTT CTCAGAAGTA 
GCACTTGCTG GTTTGGCAAG CAAGGAGAAC TTCAGCAACG TTAGTCTGCG GAGTGTTAAC 
CTAACAGAGC AAAATTCTAA CAACAGTGCT GTGCCATACA AGAAGTTAAT GCTGCTGCAA 
ATTAAAGGAA GAAGACATGT TCAAACAAGA TTAGTTGAAC CAAGGGCCTC ATCACTGAAC 
AGTGGAGACT GTTTCCTATT GTTAACTCCA CATCTCTGTT TCTTATGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAAGC AAAGGCTTCA GAACTTGCAA CTTTGATTCA GACAAAGAGG 
GAACTTGGCT GTAGAGCTTC TTATATACAG ACTATAGAAG AAGGAATAAA CACCCATACC 
CATGCAGCCA AAGACTTCTG GAAACTCCTT GGTGGCCAAG CAAATTACCA GTCTGCTGGA 
AGACCTGAAG AGGATGAAAT GTATGAAGCT GCCATAATAG AAACTAATTG CATTTACCGT 
CTTGTAGAGG ATAAACTCAT TCCTGAAGAT GACTACTGGG GAAAGATGCC AAAATGTACC 
CTGCTACAAC CAAAAGAGGT GCTCGTATTT GATTTTGGCA GTGAAGTGTA TGTCTGGCAT 
GGAAAAGAAG TTACTTTAGC ACAAAGAAAA GTGGCATTCC AGCTGGCAAA ACACTTATGG 
AATGGCACCT TCGACTACTC AAATTGTGAC ATAAATCCTC TGGACCCAGG AGAATGCAAT 
CCCCTCATAC CCAGAAAGGG ACAAGGACGT CCAGACTGGG CTGTGTTTGG CAGGCTCACA 
GAACATAATG AGACCATACT GTTCAAAGAA AAATTCCTTG ATTGGACAGA GCTGAAGAAA 
CTCAATGAGA AGAATTCTAG TGAATCCCTT CACCAGAAGG AAGAATCCAA ATCTGATTCT 
AAACCATATG ATGTCATGCT AATGGTACCT GTGCCCCAAA CTGCTGTAGG CACGGTTCTG 
GATGGAATGA ATATTGGCCG GGGCTATGGA CTTGTAGAAG GAGAAGATGG GAGGCAGTTT 
GAAATTATCA CTGCATCTGT GGATGTCTGG CACATCCTGG AGTTTGATTA TAGTAGATTG 
CCTAAACAAA GCATAGGACA GTTCCATGAG GGAGACACAT ACGTGGTGAA GTGGAAGTAC 
ATGGTGAGCA CTACAGTTGG AAGCAGACAA AAAGGAGAGC AACAAGTAAG AGCTGTTGGA 
AAAGAGAAAT GTGTGTATTT CTTTTGGCAA GGAAGGCACT CCACAGTGAG TGAGAAAGGG 
ACATCGGCAC TGATGACAGT GGAACTTGAT GAGGAGAGAG GAGCACAGGT ACAAGTGCTT 
CAAGGGAAAG AACTACCATG CTTCCTGCAG TGCTTCCAAG GAGGGATGAT TGTGCATGCT 
GGAAGAAGGG AAGAGGAAGA AGAAAATGCA CAAAGTGACT GGAGACTATA TTGCGTGCGA 
GGAGAAGTTC CCAATGAAGG AAACTTACTT GAAGTAGCAT GTCACTGCAG CAGCCTGCGT 
TCCCGGACAT CCATGATTGT CCTCAATATA AATAAAGCTC TTATCTACCT GTGGCATGGC 
TGCAAAGCAC AATCTCACAC CAAGGATGTA GGAAGAACAG CAGCCAATAA AATAAAAGAA 
CAATGTCCGC TGGAAGCAGG GCTGCACAGC AGCAGCAAGG TGACAATACA CGAATGTGAT 
GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT GCATTAGGAA GGCGAGATCG AAAGGCCTAT 
GATTGCATGT TGCAAGATCC GGGAAAGTTT AATTTCACCC CCCGCCTCTT CAGCCTCAGT 
AGTTCTTCGG GAGAATTCTC AGCCACTGAG TACGTTTACC CTTCAAGAGA CCCTACTGTC 
ATCAATTCTA TGCCCTTCTT GCAAGAGGAT CTTTACACTG CCCCACAGCC AGCACTGTTC 
CTTGTTGACA ATCACCATGA AGTGTACCTG TGGCAAGGCT GGTGGCCTGT GGAAAACAAA 
ATCACTGGAT CAGCTCGAAT CAGGTGGGCT ACAGACAGGA AATGCGCCAT GGAGACCGTG 
CTCCAGTACT GCAAAGGAAA AAATGTAAAG AAGCCCCCAA AGTCCTATCT CATTCATGCC 
GGCCTAGAGC CTCTGACCTT CACTAATATG TTCCCAAGTT GGGAACACAG GGAAGACATT 
GCAGAGATCA CAGAAATGGA TGCTGACGTT TCTAATCAGA TAATCCTTGT AGAAGATGTG 
CTGGCCAAGC TGTGTAAAAC TGTGTATCCC CTAGCTGATC TCTTAGCCAG GCCTTTACCT 
GAGGGAGTTG ATCCTCTGAA GCTTGAAATT TATCTTTCAG ATGAGGACTT TGAGGTTGCA 
TTAGAGATGA CAAGAGAAGA GTACAATGCG CTGCCATCTT GGAAGCAGGT TAATCTGAAA 
AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137596.1
CDS	14..5674
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282110.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282110.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGGTC TATTATGTGT 
GCTGAAATAC CAACTGCTGA CGATGAAGAA AAATTAGATG ACCGAGCCAA ACTAAGTGTA 
GCTGCAAAGA GACTGCTTTT TAGAGAAATG GAAAAATCTT TTGAAATGAA GAATATTCCT 
AAACCACCTA CGAGAAGCTC AGCAGTAGAG AGAAGAATGC GGCGTTTGCA GGACAGATCA 
CACACGCAGC CTATTACCAG TGAGGAAGTG GTCATTGCAG CTACCTTGCA GGCATCAGCA 
CACCAAAAAA TACTAGCTAA AGAGGAGATA AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT 
CAATCCGATG AACCAGATTC TTCCACATTA AGCTTGGCAG AAAAGATGGC TTTGTTTAAC 
AAACTGTCTC AGCCAGTATC CAGGGCTATA TCCACAAGGA GTCGGGGAGA TATTAGGCAC 
AGAAGGATGA ATACTCGTTA CCAGACCCAG CCAGTCACTC TTGGAGAAGT TGAGCAGGTG 
CAAAACGAAA GTGGAAAACT AACTCCCCTG TCAAGTACAG TTTCAACATC AGTTTCAGCA 
ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT GGAGACATGC ATGCGAAGTC ACGTAGTGAT 
GGAAGTGTGA GCGCTGCCAC AAAAGATGAG TTGAGATTCC ATGCTTCAGC AGAAAGCTCA 
GACTCATCAG GAAAACGCAC ACAGAAGTCA GAACAGTGGC AGCCCTCAAT GGAAGTGCTG 
GAAAGCAAAA GAGCATCAAA GAAGCATGAA GAAGAAGGAA AGAGGTTTCT AGCACATGAG 
GCTAATGAAA TCAGAAAGTA CAGCTCCTTT GAGGATGAAA CCACACATCC TGTTCTTGAA 
AAAACAGGAG ACTACCATAA AGAGGCAGCC TACAGCTTCC TGAGGAAGGG CAGCATGGAA 
CTGTTTAGTT CACAACCACT TTTTCAGCCT CTTGAAAGGA AGGAAATTGA TACCATCATG 
CAAGACCACG TGGAGCCTAC CTCTGAACTC ACCAGCACAC CAGCAACGAG GACACTTTCC 
CAGACTACAG CTGCAGCATC CTGTAGGCTA CAGGAGCTGT CTGAACAACT GGAGGGAAAG 
TTCTACAAGA ATTCCTGTGA GATGTTTTCT GCTGGAGAGA ACAAAATACA GACAACTGAG 
GATGCCACCG ATAGCTCCAG CAAAACGATG TCAATTAAAG AAAGGTTGGC ATTGCTGAAG 
AAAAGTGGTG AAGAAGATTG GAAGAGCAGG CTCAGTAAAA AGCAGGAATA TGCAAAAGTG 
TCTGCCACTG ATCGTAGCGC ACAGATGCAA GAAGTTGAGC AGCTGTTGAA AAAGGAACCT 
GTATATGCTT CTACTTACTC TCCTGTTATA CCTGCTGTCC ATAAATTTCA TCCTTTTCTA 
CCTATTAATC AGCAGAGGAT AGCAGATAAT CAGGAGAGTC AAATGACTAT AGAAGAAAGA 
AAACACCTCA TCACAGCTCG AGAAGAAGCC TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT 
GACTCCACGC AGTTCACCGT AGCTGGTCGA ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT 
GCACTAACAC CAGTAGCATC CCCTTATGGT AACAGGCAGA AGTCTACAAC TCCGGTCACA 
AAGCCCTTGG AAGAAATTGA AGCTAGGCCT GATATGCAAT TGGAATCAGA TATGAAGCTT 
GACAAGCTGG AGTCATTCTT GGGAAGGCTG AATAACAAAG TCGCTGGAAT GCAAGAGACA 
GTGTTGACAG TTACAGGAAA ATCTGTGAAA GAGGTGATGA AGCTGGATGA TGATGAAACA 
TTTTCCAAAT TTTACCGCCG TGTGGATTTC CCTTCCTCCT CTGTGCCTTT GGACCTTGAT 
GAGGACTTTG ATGCCATCTT TGACCCTTAT GCACCAAAGT TAATATCCTC TGTAGCAGAG 
CACAAACGTG CAGTGAGGCC TATGCGCAGG GTTCAGTCCT CCAGAAACCC CCTGAAAATG 
CTGGCAGCAA GAGAAGATAT TCTTCATGAG TACACTGAAC AAAGACTGAA TGTTGCCTTC 
ATGGAGTCAA AGCGAATGAA AGTAGAAAAA ATGTCTGCAA ACTCAAATTT CTCAGAAGTA 
GCACTTGCTG GTTTGGCAAG CAAGGAGAAC TTCAGCAACG TTAGTCTGCG GAGTGTTAAC 
CTAACAGAGC AAAATTCTAA CAACAGTGCT GTGCCATACA AGAAGTTAAT GCTGCTGCAA 
ATTAAAGGAA GAAGACATGT TCAAACAAGA TTAGTTGAAC CAAGGGCCTC ATCACTGAAC 
AGTGGAGACT GTTTCCTATT GTTAACTCCA CATCTCTGTT TCTTATGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAAGC AAAGGCTTCA GAACTTGCAA CTTTGATTCA GACAAAGAGG 
GAACTTGGCT GTAGAGCTTC TTATATACAG ACTATAGAAG AAGGAATAAA CACCCATACC 
CATGCAGCCA AAGACTTCTG GAAACTCCTT GGTGGCCAAG CAAATTACCA GTCTGCTGGA 
AGACCTGAAG AGGATGAAAT GTATGAAGCT GCCATAATAG AAACTAATTG CATTTACCGT 
CTTGTAGAGG ATAAACTCAT TCCTGAAGAT GACTACTGGG GAAAGATGCC AAAATGTACC 
CTGCTACAAC CAAAAGAGGT GCTCGTATTT GATTTTGGCA GTGAAGTGTA TGTCTGGCAT 
GGAAAAGAAG TTACTTTAGC ACAAAGAAAA GTGGCATTCC AGCTGGCAAA ACACTTATGG 
AATGGCACCT TCGACTACTC AAATTGTGAC ATAAATCCTC TGGACCCAGG AGAATGCAAT 
CCCCTCATAC CCAGAAAGGG ACAAGGACGT CCAGACTGGG CTGTGTTTGG CAGGCTCACA 
GAACATAATG AGACCATACT GTTCAAAGAA AAATTCCTTG ATTGGACAGA GCTGAAGAAA 
CTCAATGAGA AGAATTCTAG TGAATCCCTT CACCAGAAGG AAGAATCCAA ATCTGATTCT 
AAACCATATG ATGTCATGCT AATGGTACCT GTGCCCCAAA CTGCTGTAGG CACGGTTCTG 
GATGGAATGA ATATTGGCCG GGGCTATGGA CTTGTAGAAG GAGAAGATGG GAGGCAGTTT 
GAAATTATCA CTGCATCTGT GGATGTCTGG CACATCCTGG AGTTTGATTA TAGTAGATTG 
CCTAAACAAA GCATAGGACA GTTCCATGAG GGAGACACAT ACGTGGTGAA GTGGAAGTAC 
ATGGTGAGCA CTACAGTTGG AAGCAGACAA AAAGGAGAGC AACAAGTAAG AGCTGTTGGA 
AAAGAGAAAT GTGTGTATTT CTTTTGGCAA GGAAGGCACT CCACAGTGAG TGAGAAAGGG 
ACATCGGCAC TGATGACAGT GGAACTTGAT GAGGAGAGAG GAGCACAGGT ACAAGTGCTT 
CAAGGGAAAG AACTACCATG CTTCCTGCAG TGCTTCCAAG GAGGGATGAT TGTGCATGCT 
GGAAGAAGGG AAGAGGAAGA AGAAAATGCA CAAAGTGACT GGAGACTATA TTGCGTGCGA 
GGAGAAGTTC CCAATGAAGG AAACTTACTT GAAGTAGCAT GTCACTGCAG CAGCCTGCGT 
TCCCGGACAT CCATGATTGT CCTCAATATA AATAAAGCTC TTATCTACCT GTGGCATGGC 
TGCAAAGCAC AATCTCACAC CAAGGATGTA GGAAGAACAG CAGCCAATAA AATAAAAGAA 
CAATGTCCGC TGGAAGCAGG GCTGCACAGC AGCAGCAAGG TGACAATACA CGAATGTGAT 
GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT GCATTAGGAA GGCGAGATCG AAAGGCCTAT 
GATTGCATGT TGCAAGATCC GGGAAAGTTT AATTTCACCC CCCGCCTCTT CAGCCTCAGT 
AGTTCTTCGG GAGAATTCTC AGCCACTGAG TACGTTTACC CTTCAAGAGA CCCTACTGTC 
ATCAATTCTA TGCCCTTCTT GCAAGAGGAT CTTTACACTG CCCCACAGCC AGCACTGTTC 
CTTGTTGACA ATCACCATGA AGTGTACCTG TGGCAAGGCT GGTGGCCTGT GGAAAACAAA 
ATCACTGGAT CAGCTCGAAT CAGGTGGGCT ACAGACAGGA AATGCGCCAT GGAGACCGTG 
CTCCAGTACT GCAAAGGAAA AAATGTAAAG AAGCCCCCAA AGTCCTATCT CATTCATGCC 
GGCCTAGAGC CTCTGACCTT CACTAATATG TTCCCAAGTT GGGAACACAG GGAAGACATT 
GCAGAGATCA CAGAAATGGA TGCTGACGTT TCTAATCAGA TAATCCTTGT AGAAGATGTG 
CTGGCCAAGC TGTGTAAAAC TGTGTATCCC CTAGCTGATC TCTTAGCCAG GCCTTTACCT 
GAGGGAGTTG ATCCTCTGAA GCTTGAAATT TATCTTTCAG ATGAGGACTT TGAGGTTGCA 
TTAGAGATGA CAAGAGAAGA GTACAATGCG CTGCCATCTT GGAAGCAGGT TAATCTGAAA 
AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148336.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004104.1 Gallus gallus supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OGa146726
Clone ID Related Accession (Same CDS sequence)	XM_025148336.1
Accession Version	XM_025148336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGAGGATAG CAGATAATCA GGAGAGTCAA ATGACTATAG AAGAAAGAAA ACACCTCATC 
ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA GGTAAAGGCG CAGCAAATGA CTCCACGCAG 
TTCACCGTAG CTGGTCGAAT GGTAAAAAAA GGCCTGGCTT CTCCAAGTGC ACTAACACCA 
GTAGCATCCC CTTATGGTAA CAGGCAGAAG TCTACAACTC CGGTCACAAA GCCCTTGGAA 
GAAATTGAAG CTAGGCCTGA TATGCAATTG GAATCAGATA TGAAGCTTGA CAAGCTGGAG 
TCATTCTTGG GAAGGCTGAA TAACAAAGTC GCTGGAATGC AAGAGACAGT GTTGACAGTT 
ACAGGAAAAT CTGTGAAAGA GGTGATGAAG CTGGATGATG ATGAAACATT TTCCAAATTT 
TACCGCCGTG TGGATTTCCC TTCCTCCTCT GTGCCTTTGG ACCTTGATGA GGACTTTGAT 
GCCATCTTTG ACCCTTATGC ACCAAAGTTA ATATCCTCTG TAGCAGAGCA CAAACGTGCA 
GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC AGAAACCCCC TGAAAATGCT GGCAGCAAGA 
GAAGATATTC TTCATGAGTA CACTGAACAA AGACTGAATG TTGCCTTCAT GGAGTCAAAG 
CGAATGAAAG TAGAAAAAAT GTCTGCAAAC TCAAATTTCT CAGAAGTAGC ACTTGCTGGT 
TTGGCAAGCA AGGAGAACTT CAGCAACGTT AGTCTGCGGA GTGTTAACCT AACAGAGCAA 
AATTCTAACA ACAGTGCTGT GCCATACAAG AAGTTAATGC TGCTGCAAAT TAAAGGAAGA 
AGACATGTTC AAACAAGATT AGTTGAACCA AGGGCCTCAT CACTGAACAG TGGAGACTGT 
TTCCTATTGT TAACTCCACA TCTCTGTTTC TTATGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAAGCAA AGGCTTCAGA ACTTGCAACT TTGATTCAGA CAAAGAGGGA ACTTGGCTGT 
AGAGCTTCTT ATATACAGAC TATAGAAGAA GGAATAAACA CCCATACCCA TGCAGCCAAA 
GACTTCTGGA AACTCCTTGG TGGCCAAGCA AATTACCAGT CTGCTGGAAG ACCTGAAGAG 
GATGAAATGT ATGAAGCTGC CATAATAGAA ACTAATTGCA TTTACCGTCT TGTAGAGGAT 
AAACTCATTC CTGAAGATGA CTACTGGGGA AAGATGCCAA AATGTACCCT GCTACAACCA 
AAAGAGGTGC TCGTATTTGA TTTTGGCAGT GAAGTGTATG TCTGGCATGG AAAAGAAGTT 
ACTTTAGCAC AAAGAAAAGT GGCATTCCAG CTGGCAAAAC ACTTATGGAA TGGCACCTTC 
GACTACTCAA ATTGTGACAT AAATCCTCTG GACCCAGGAG AATGCAATCC CCTCATACCC 
AGAAAGGGAC AAGGACGTCC AGACTGGGCT GTGTTTGGCA GGCTCACAGA ACATAATGAG 
ACCATACTGT TCAAAGAAAA ATTCCTTGAT TGGACAGAGC TGAAGAAACT CAATGAGAAG 
AATTCTAGTG AATCCCTTCA CCAGAAGGAA GAATCCAAAT CTGATTCTAA ACCATATGAT 
GTCATGCTAA TGGTACCTGT GCCCCAAACT GCTGTAGGCA CGGTTCTGGA TGGAATGAAT 
ATTGGCCGGG GCTATGGACT TGTAGAAGGA GAAGATGGGA GGCAGTTTGA AATTATCACT 
GCATCTGTGG ATGTCTGGCA CATCCTGGAG TTTGATTATA GTAGATTGCC TAAACAAAGC 
ATAGGACAGT TCCATGAGGG AGACACATAC GTGGTGAAGT GGAAGTACAT GGTGAGCACT 
ACAGTTGGAA GCAGACAAAA AGGAGAGCAA CAAGTAAGAG CTGTTGGAAA AGAGAAATGT 
GTGTATTTCT TTTGGCAAGG AAGGCACTCC ACAGTGAGTG AGAAAGGGAC ATCGGCACTG 
ATGACAGTGG AACTTGATGA GGAGAGAGGA GCACAGGTAC AAGTGCTTCA AGGGAAAGAA 
CTACCATGCT TCCTGCAGTG CTTCCAAGGA GGGATGATTG TGCATGCTGG AAGAAGGGAA 
GAGGAAGAAG AAAATGCACA AAGTGACTGG AGACTATATT GCGTGCGAGG AGAAGTTCCC 
AATGAAGGAA ACTTACTTGA AGTAGCATGT CACTGCAGCA GCCTGCGTTC CCGGACATCC 
ATGATTGTCC TCAATATAAA TAAAGCTCTT ATCTACCTGT GGCATGGCTG CAAAGCACAA 
TCTCACACCA AGGATGTAGG AAGAACAGCA GCCAATAAAA TAAAAGAACA ATGTCCGCTG 
GAAGCAGGGC TGCACAGCAG CAGCAAGGTG ACAATACACG AATGTGATGA AGGGTCAGAG 
CCTTTGGGAT TCTGGGATGC ATTAGGAAGG CGAGATCGAA AGGCCTATGA TTGCATGTTG 
CAAGATCCGG GAAAGTTTAA TTTCACCCCC CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA 
GAATTCTCAG CCACTGAGTA CGTTTACCCT TCAAGAGACC CTACTGTCAT CAATTCTATG 
CCCTTCTTGC AAGAGGATCT TTACACTGCC CCACAGCCAG CACTGTTCCT TGTTGACAAT 
CACCATGAAG TGTACCTGTG GCAAGGCTGG TGGCCTGTGG AAAACAAAAT CACTGGATCA 
GCTCGAATCA GGTGGGCTAC AGACAGGAAA TGCGCCATGG AGACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG TCCTATCTCA TTCATGCCGG CCTAGAGCCT 
CTGACCTTCA CTAATATGTT CCCAAGTTGG GAACACAGGG AAGACATTGC AGAGATCACA 
GAAATGGATG CTGACGTTTC TAATCAGATA ATCCTTGTAG AAGATGTGCT GGCCAAGCTG 
TGTAAAACTG TGTATCCCCT AGCTGATCTC TTAGCCAGGC CTTTACCTGA GGGAGTTGAT 
CCTCTGAAGC TTGAAATTTA TCTTTCAGAT GAGGACTTTG AGGTTGCATT AGAGATGACA 
AGAGAAGAGT ACAATGCGCT GCCATCTTGG AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA 
CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004104.1
CDS	20..6928
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148336.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148336.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGAGGATAG CAGATAATCA GGAGAGTCAA ATGACTATAG AAGAAAGAAA ACACCTCATC 
ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA GGTAAAGGCG CAGCAAATGA CTCCACGCAG 
TTCACCGTAG CTGGTCGAAT GGTAAAAAAA GGCCTGGCTT CTCCAAGTGC ACTAACACCA 
GTAGCATCCC CTTATGGTAA CAGGCAGAAG TCTACAACTC CGGTCACAAA GCCCTTGGAA 
GAAATTGAAG CTAGGCCTGA TATGCAATTG GAATCAGATA TGAAGCTTGA CAAGCTGGAG 
TCATTCTTGG GAAGGCTGAA TAACAAAGTC GCTGGAATGC AAGAGACAGT GTTGACAGTT 
ACAGGAAAAT CTGTGAAAGA GGTGATGAAG CTGGATGATG ATGAAACATT TTCCAAATTT 
TACCGCCGTG TGGATTTCCC TTCCTCCTCT GTGCCTTTGG ACCTTGATGA GGACTTTGAT 
GCCATCTTTG ACCCTTATGC ACCAAAGTTA ATATCCTCTG TAGCAGAGCA CAAACGTGCA 
GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC AGAAACCCCC TGAAAATGCT GGCAGCAAGA 
GAAGATATTC TTCATGAGTA CACTGAACAA AGACTGAATG TTGCCTTCAT GGAGTCAAAG 
CGAATGAAAG TAGAAAAAAT GTCTGCAAAC TCAAATTTCT CAGAAGTAGC ACTTGCTGGT 
TTGGCAAGCA AGGAGAACTT CAGCAACGTT AGTCTGCGGA GTGTTAACCT AACAGAGCAA 
AATTCTAACA ACAGTGCTGT GCCATACAAG AAGTTAATGC TGCTGCAAAT TAAAGGAAGA 
AGACATGTTC AAACAAGATT AGTTGAACCA AGGGCCTCAT CACTGAACAG TGGAGACTGT 
TTCCTATTGT TAACTCCACA TCTCTGTTTC TTATGGGTAG GAGAGTTTGC AAATGTCATA 
GAAAAAGCAA AGGCTTCAGA ACTTGCAACT TTGATTCAGA CAAAGAGGGA ACTTGGCTGT 
AGAGCTTCTT ATATACAGAC TATAGAAGAA GGAATAAACA CCCATACCCA TGCAGCCAAA 
GACTTCTGGA AACTCCTTGG TGGCCAAGCA AATTACCAGT CTGCTGGAAG ACCTGAAGAG 
GATGAAATGT ATGAAGCTGC CATAATAGAA ACTAATTGCA TTTACCGTCT TGTAGAGGAT 
AAACTCATTC CTGAAGATGA CTACTGGGGA AAGATGCCAA AATGTACCCT GCTACAACCA 
AAAGAGGTGC TCGTATTTGA TTTTGGCAGT GAAGTGTATG TCTGGCATGG AAAAGAAGTT 
ACTTTAGCAC AAAGAAAAGT GGCATTCCAG CTGGCAAAAC ACTTATGGAA TGGCACCTTC 
GACTACTCAA ATTGTGACAT AAATCCTCTG GACCCAGGAG AATGCAATCC CCTCATACCC 
AGAAAGGGAC AAGGACGTCC AGACTGGGCT GTGTTTGGCA GGCTCACAGA ACATAATGAG 
ACCATACTGT TCAAAGAAAA ATTCCTTGAT TGGACAGAGC TGAAGAAACT CAATGAGAAG 
AATTCTAGTG AATCCCTTCA CCAGAAGGAA GAATCCAAAT CTGATTCTAA ACCATATGAT 
GTCATGCTAA TGGTACCTGT GCCCCAAACT GCTGTAGGCA CGGTTCTGGA TGGAATGAAT 
ATTGGCCGGG GCTATGGACT TGTAGAAGGA GAAGATGGGA GGCAGTTTGA AATTATCACT 
GCATCTGTGG ATGTCTGGCA CATCCTGGAG TTTGATTATA GTAGATTGCC TAAACAAAGC 
ATAGGACAGT TCCATGAGGG AGACACATAC GTGGTGAAGT GGAAGTACAT GGTGAGCACT 
ACAGTTGGAA GCAGACAAAA AGGAGAGCAA CAAGTAAGAG CTGTTGGAAA AGAGAAATGT 
GTGTATTTCT TTTGGCAAGG AAGGCACTCC ACAGTGAGTG AGAAAGGGAC ATCGGCACTG 
ATGACAGTGG AACTTGATGA GGAGAGAGGA GCACAGGTAC AAGTGCTTCA AGGGAAAGAA 
CTACCATGCT TCCTGCAGTG CTTCCAAGGA GGGATGATTG TGCATGCTGG AAGAAGGGAA 
GAGGAAGAAG AAAATGCACA AAGTGACTGG AGACTATATT GCGTGCGAGG AGAAGTTCCC 
AATGAAGGAA ACTTACTTGA AGTAGCATGT CACTGCAGCA GCCTGCGTTC CCGGACATCC 
ATGATTGTCC TCAATATAAA TAAAGCTCTT ATCTACCTGT GGCATGGCTG CAAAGCACAA 
TCTCACACCA AGGATGTAGG AAGAACAGCA GCCAATAAAA TAAAAGAACA ATGTCCGCTG 
GAAGCAGGGC TGCACAGCAG CAGCAAGGTG ACAATACACG AATGTGATGA AGGGTCAGAG 
CCTTTGGGAT TCTGGGATGC ATTAGGAAGG CGAGATCGAA AGGCCTATGA TTGCATGTTG 
CAAGATCCGG GAAAGTTTAA TTTCACCCCC CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA 
GAATTCTCAG CCACTGAGTA CGTTTACCCT TCAAGAGACC CTACTGTCAT CAATTCTATG 
CCCTTCTTGC AAGAGGATCT TTACACTGCC CCACAGCCAG CACTGTTCCT TGTTGACAAT 
CACCATGAAG TGTACCTGTG GCAAGGCTGG TGGCCTGTGG AAAACAAAAT CACTGGATCA 
GCTCGAATCA GGTGGGCTAC AGACAGGAAA TGCGCCATGG AGACCGTGCT CCAGTACTGC 
AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG TCCTATCTCA TTCATGCCGG CCTAGAGCCT 
CTGACCTTCA CTAATATGTT CCCAAGTTGG GAACACAGGG AAGACATTGC AGAGATCACA 
GAAATGGATG CTGACGTTTC TAATCAGATA ATCCTTGTAG AAGATGTGCT GGCCAAGCTG 
TGTAAAACTG TGTATCCCCT AGCTGATCTC TTAGCCAGGC CTTTACCTGA GGGAGTTGAT 
CCTCTGAAGC TTGAAATTTA TCTTTCAGAT GAGGACTTTG AGGTTGCATT AGAGATGACA 
AGAGAAGAGT ACAATGCGCT GCCATCTTGG AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA 
CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148337.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004105.1 Gallus gallus supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OGa146727
Clone ID Related Accession (Same CDS sequence)	XM_025148337.1
Accession Version	XM_025148337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGAA ATCCTTGGAT CTCCAGCTAT TTGGGAAATG TTAAACACTC TGAGGAGCAA 
GATTTTAACA AAGGAAACTC GGTTAGATTT AAACCTTCAC GACTGCTCCA CAGGAAGAGA 
GCGTTAAGTT TTCAGAAGGA GTTCTCATTT GAAGATAAAG AAGAACTTGC AAACAGCACA 
AAGGATATTG ATGCAAACCT CTTAGCAGTA CTTCCAAAAG TTTCAGAATT AAGAAAACTG 
TTTGAACCAA ACAACCAAGA GTTTTTGGAA ATGAAAAGAA AGGAGAGAAT AGCTAGACGC 
TTAGAGGGAA TTGAAAATGA TACGCAACCT ATGCTTCTAC AGAACTGCCC AGGGTCAGTC 
ACACACCGTT TACTAGAAGA AGACACACCA AGGTATATGC GTGCAACTGA TCCATACAGT 
CATCACTTAG GAAGATCAAA TGAAGAGGAG GAAGCTTCAG ATTCTTCTGT AGAGAAACAA 
CCCAGATCAT CCAGATACCG CACAGAAACC ACAGGAGGAA ATACAGAGTC TTTGTACAGT 
ACAGGAAACA TGGATACCCA TGAACTAGAG TCTAAAGCAG AAAGAATAGC TAGGTACAAG 
GCAGAGAGAC GGCGCCAACT GGCAGAAAAG TATGGATTAT CTCTTGATTC TGATTTGGAT 
TCTGACTACT CATCCAGATA TTCCCGGGCA AGAAAAGATC CTGACAGTGT GGAAAGAAAG 
GCATTGAGAA GTGAAAGGCA TGATGATGAA AACAAGGACT CTGGCTCCTT GTATTTGTCC 
AGGACTGAGG TGAAGGAGTC AAAGAGTGTG CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT 
GAAAAAGATG GTATTCCAGC TAAAGAAGAG CTTTCAAATG AAAAGAGTGA TAAAAGAGCA 
GATGATGACA GTGCCATTAG ACAGGCTTCT GACCCTTCAG CAACATTGGA TAGTTCTGTC 
TCACTAACAC TTTCTGGACG AGAGTCTTCC TCCTGTAATG AAGTGCCAAT ATCACCAAAG 
CAAAGCCCCC GAGAGTCTCT TTCTTCACCA AAGCGGGCAG CCTCACCAAT CCATTTGCAG 
AATGATCAGC CCTTGCACAG TAACATCAGA CAAAGTAGTG CAGGGAAAGC AAAACCTGAA 
TGGTTCCTTC AGAAAGATTC AGAAGGGGAC ACACCATCTC TTATCAGCTG GCCCTCCAGA 
GTAAAAGTTA GAGAGAAACT GGTGAAAGAG GAAAGTGCAC GTGGAAGCCC TGAACCTGTC 
ACAGACAGTG TATCTCAGAG AAAATCCCAG CCAGTGTCAG CCCACTACAT GCCTCTTCAG 
ACAGAAACGT CTGCCTTTGA TAGGGTTCTC AATCAGCCCG TCAGTGCAGT CCGCCAACCA 
ATACATGGGT ATATTCAGCC TGCTGGTGTT GCACACACGG CTCAGCTGAT GGCAACAGAA 
GAACCAGAAA GCATCTTCGT TGGCAGCCGC CTCCTTCCAG ACAGTGTCAG CCCCTTAACG 
AAATCAGCAG CAGCCACTGC ACCAGAGGGT GAAAAGAAAG AGACGCTGGC TTATGGAGCA 
AAGCCTGGCG AAGAAGAATC CCCAGAGGGT GAGAAGGCTT CCAAAGGCAG AAGACCAATT 
TTGCCATCTG AGATTCGCAA GCAAGGGAAG AGCCACGAAG GCCTCTTTGG AGGAGAAGAA 
CCAGATACCA GTGTGGGGAG CTCCTCATCT GCAAGCAAGC CGAAGGTTGG CTCAGATGTT 
CAGCAGGAGC AGGAATGCAA AAAGAAGCAG GCGCCTGAGC AGCAGCTGAA GACACCTGAG 
AACATGGAGA GGAGCGAAGC TGTCATGTAC ATACAGAGCG GGTCTGTATC AAAGCCAGCC 
CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA AAGCATAAAG TCCTGACTCG CTCCATGTCT 
GACTATACTG TGCCTCCTAA GCTAGAAGCT TTTCATAGTA AGAGCATGGA AGCAAACGCA 
GCAAATGGTG AGATTGGCAT GGTTGACACA AAGGTCTCTG TTGCCCAGCT CCGTAATGTC 
TTTCTGGAAA CTGCTCATGC CAACAAGAAA ACGGACCTCG AATCTCGGTT TGAGATGTCA 
ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT GACCGTGAAA GAGGGCCGCG AAGGCCGAGG 
CGCTATTTCA CTCCTGGTGA AAATAGAAAG ACTTCTGAGC GATTTAAAAC CCAACCTATA 
ACGTCAGCAG AACGAAAGGA GACGGATAGG TCTATTATGT GTGCTGAAAT ACCAACTGCT 
GACGATGAAG AAAAATTAGA TGACCGAGCC AAACTAAGTG TAGCTGCAAA GAGACTGCTT 
TTTAGAGAAA TGGAAAAATC TTTTGAAATG AAGAATATTC CTAAACCACC TACGAGAAGC 
TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG CAGGACAGAT CACACACGCA GCCTATTACC 
AGTGAGGAAG TGGTCATTGC AGCTACTGAA ACTACCCCTG CTTCACGTTC TGTGAATACC 
CACACAGTAG TGGCAAGAGT ACCTAGTCCC ACTCTAGTTA AGAGCACTGT ACAACCTACC 
AGCTTGCAGG CATCAGCACA CCAAAAAATA CTAGCTAAAG AGGAGATAAG AGCAGCCAAA 
GAGGCGATGG GGCAAGATCA ATCCGATGAA CCAGATTCTT CCACATTAAG CTTGGCAGAA 
AAGATGGCTT TGTTTAACAA ACTGTCTCAG CCAGTATCCA GGGCTATATC CACAAGGAGT 
CGGGGAGATA TTAGGCACAG AAGGATGAAT ACTCGTTACC AGACCCAGCC AGTCACTCTT 
GGAGAAGTTG AGCAGATGCA ATGGAAAAAG AGTAAACCGG TGCAAAACGA AAGTGGAAAA 
CTAACTCCCC TGTCAAGTAC AGTTTCAACA TCAGTTTCAG CAATGGCTTC TGCCCTTTCT 
GCACTGTTTG CTGGAGACAT GCATGCGAAG TCACGTAGTG ATGGAAGTGT GAGCGCTGCC 
ACAAAAGATG AGTTGAGATT CCATGCTTCA GCAGAAAGCT CAGACTCATC AGGAAAACGC 
ACACAGAAGT CAGAACAGTG GCAGCCCTCA ATGGAAGTGC TGGAAAGCAA AAGAGCATCA 
AAGAAGCATG AAGAAGAAGG AAAGAGGTTT CTAGCACATG AGGCTAATGA AATCAGAAAG 
TACAGCTCCT TTGAGGATGA AACCACACAT CCTGTTCTTG AAAAAACAGG AGACTACCAT 
AAAGAGGCAG CCTACAGCTT CCTGAGGAAG GGCAGCATGG AACTGTTTAG TTCACAACCA 
CTTTTTCAGC CTCTTGAAAG GAAGGAAATT GATACCATCA TGCAAGACCA CGTGGAGCCT 
ACCTCTGAAC TCACCAGCAC ACCAGCAACG AGGACACTTT CCCAGACTAC AGCTGCAGCA 
TCCTGTAGGC TACAGGAGCT GTCTGAACAA CTGGAGGGAA AGTTCTACAA GAATTCCTGT 
GAGATGTTTT CTGCTGGAGA GAACAAAATA CAGACAACTG AGGATGCCAC CGATAGCTCC 
AGCAAAACGA TGTCAATTAA AGAAAGGTTG GCATTGCTGA AGAAAAGTGG TGAAGAAGAT 
TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA TATGCAAAAG TGTCTGCCAC TGATCGTAGC 
GCACAGATGC AAGAAGTTGA GCAGCTGTTG AAAAAGGAAC CTGTATATGC TTCTACTTAC 
TCTCCTGTTA TACCTGCTGT CCATAAATTT CATCCTTTTC TACCTATTAA TCAGAGGATA 
GCAGATAATC AGGAGAGTCA AATGACTATA GAAGAAAGAA AACACCTCAT CACAGCTCGA 
GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC GCAGCAAATG ACTCCACGCA GTTCACCGTA 
GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT TCTCCAAGTG CACTAACACC AGTAGCATCC 
CCTTATGGTA ACAGGCAGAA GTCTACAACT CCGGTCACAA AGCCCTTGGA AGAAATTGAA 
GCTAGGCCTG ATATGCAATT GGAATCAGAT ATGAAGCTTG ACAAGCTGGA GTCATTCTTG 
GGAAGGCTGA ATAACAAAGT CGCTGGAATG CAAGAGACAG TGTTGACAGT TACAGGAAAA 
TCTGTGAAAG AGGTGATGAA GCTGGATGAT GATGAAACAT TTTCCAAATT TTACCGCCGT 
GTGGATTTCC CTTCCTCCTC TGTGCCTTTG GACCTTGATG AGGACTTTGA TGCCATCTTT 
GACCCTTATG CACCAAAGTT AATATCCTCT GTAGCAGAGC ACAAACGTGC AGTGAGGCCT 
ATGCGCAGGG TTCAGTCCTC CAGAAACCCC CTGAAAATGC TGGCAGCAAG AGAAGATATT 
CTTCATGAGT ACACTGAACA AAGACTGAAT GTTGCCTTCA TGGAGTCAAA GCGAATGAAA 
GTAGAAAAAA TGTCTGCAAA CTCAAATTTC TCAGAAGTAG CACTTGCTGG TTTGGCAAGC 
AAGGAGAACT TCAGCAACGT TAGTCTGCGG AGTGTTAACC TAACAGAGCA AAATTCTAAC 
AACAGTGCTG TGCCATACAA GAAGTTAATG CTGCTGCAAA TTAAAGGAAG AAGACATGTT 
CAAACAAGAT TAGTTGAACC AAGGGCCTCA TCACTGAACA GTGGAGACTG TTTCCTATTG 
TTAACTCCAC ATCTCTGTTT CTTATGGGTA GGAGAGTTTG CAAATGTCAT AGAAAAAGCA 
AAGGCTTCAG AACTTGCAAC TTTGATTCAG ACAAAGAGGG AACTTGGCTG TAGAGCTTCT 
TATATACAGA CTATAGAAGA AGGAATAAAC ACCCATACCC ATGCAGCCAA AGACTTCTGG 
AAACTCCTTG GTGGCCAAGC AAATTACCAG TCTGCTGGAA GACCTGAAGA GGATGAAATG 
TATGAAGCTG CCATAATAGA AACTAATTGC ATTTACCGTC TTGTAGAGGA TAAACTCATT 
CCTGAAGATG ACTACTGGGG AAAGATGCCA AAATGTACCC TGCTACAACC AAAAGAGGTG 
CTCGTATTTG ATTTTGGCAG TGAAGTGTAT GTCTGGCATG GAAAAGAAGT TACTTTAGCA 
CAAAGAAAAG TGGCATTCCA GCTGGCAAAA CACTTATGGA ATGGCACCTT CGACTACTCA 
AATTGTGACA TAAATCCTCT GGACCCAGGA GAATGCAATC CCCTCATACC CAGAAAGGGA 
CAAGGACGTC CAGACTGGGC TGTGTTTGGC AGGCTCACAG AACATAATGA GACCATACTG 
TTCAAAGAAA AATTCCTTGA TTGGACAGAG CTGAAGAAAC TCAATGAGAA GAATTCTAGT 
GAATCCCTTC ACCAGAAGGA AGAATCCAAA TCTGATTCTA AACCATATGA TGTCATGCTA 
ATGGTACCTG TGCCCCAAAC TGCTGTAGGC ACGGTTCTGG ATGGAATGAA TATTGGCCGG 
GGCTATGGAC TTGTAGAAGG AGAAGATGGG AGGCAGTTTG AAATTATCAC TGCATCTGTG 
GATGTCTGGC ACATCCTGGA GTTTGATTAT AGTAGATTGC CTAAACAAAG CATAGGACAG 
TTCCATGAGG GAGACACATA CGTGGTGAAG TGGAAGTACA TGGTGAGCAC TACAGTTGGA 
AGCAGACAAA AAGGAGAGCA ACAAGTAAGA GCTGTTGGAA AAGAGAAATG TGTGTATTTC 
TTTTGGCAAG GAAGGCACTC CACAGTGAGT GAGAAAGGGA CATCGGCACT GATGACAGTG 
GAACTTGATG AGGAGAGAGG AGCACAGGTA CAAGTGCTTC AAGGGAAAGA ACTACCATGC 
TTCCTGCAGT GCTTCCAAGG AGGGATGATT GTGCATGCTG GAAGAAGGGA AGAGGAAGAA 
GAAAATGCAC AAAGTGACTG GAGACTATAT TGCGTGCGAG GAGAAGTTCC CAATGAAGGA 
AACTTACTTG AAGTAGCATG TCACTGCAGC AGCCTGCGTT CCCGGACATC CATGATTGTC 
CTCAATATAA ATAAAGCTCT TATCTACCTG TGGCATGGCT GCAAAGCACA ATCTCACACC 
AAGGATGTAG GAAGAACAGC AGCCAATAAA ATAAAAGAAC AATGTCCGCT GGAAGCAGGG 
CTGCACAGCA GCAGCAAGGT GACAATACAC GAATGTGATG AAGGGTCAGA GCCTTTGGGA 
TTCTGGGATG CATTAGGAAG GCGAGATCGA AAGGCCTATG ATTGCATGTT GCAAGATCCG 
GGAAAGTTTA ATTTCACCCC CCGCCTCTTC AGCCTCAGTA GTTCTTCGGG AGAATTCTCA 
GCCACTGAGT ACGTTTACCC TTCAAGAGAC CCTACTGTCA TCAATTCTAT GCCCTTCTTG 
CAAGAGGATC TTTACACTGC CCCACAGCCA GCACTGTTCC TTGTTGACAA TCACCATGAA 
GTGTACCTGT GGCAAGGCTG GTGGCCTGTG GAAAACAAAA TCACTGGATC AGCTCGAATC 
AGGTGGGCTA CAGACAGGAA ATGCGCCATG GAGACCGTGC TCCAGTACTG CAAAGGAAAA 
AATGTAAAGA AGCCCCCAAA GTCCTATCTC ATTCATGCCG GCCTAGAGCC TCTGACCTTC 
ACTAATATGT TCCCAAGTTG GGAACACAGG GAAGACATTG CAGAGATCAC AGAAATGGAT 
GCTGACGTTT CTAATCAGAT AATCCTTGTA GAAGATGTGC TGGCCAAGCT GTGTAAAACT 
GTGTATCCCC TAGCTGATCT CTTAGCCAGG CCTTTACCTG AGGGAGTTGA TCCTCTGAAG 
CTTGAAATTT ATCTTTCAGA TGAGGACTTT GAGGTTGCAT TAGAGATGAC AAGAGAAGAG 
TACAATGCGC TGCCATCTTG GAAGCAGGTT AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004105.1
CDS	16..6915
Translation	MFRNPWISSYLGNVKHSEEQDFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148337.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148337.1

ORF Insert Sequence:

ATGTTCCGAA ATCCTTGGAT CTCCAGCTAT TTGGGAAATG TTAAACACTC TGAGGAGCAA 
GATTTTAACA AAGGAAACTC GGTTAGATTT AAACCTTCAC GACTGCTCCA CAGGAAGAGA 
GCGTTAAGTT TTCAGAAGGA GTTCTCATTT GAAGATAAAG AAGAACTTGC AAACAGCACA 
AAGGATATTG ATGCAAACCT CTTAGCAGTA CTTCCAAAAG TTTCAGAATT AAGAAAACTG 
TTTGAACCAA ACAACCAAGA GTTTTTGGAA ATGAAAAGAA AGGAGAGAAT AGCTAGACGC 
TTAGAGGGAA TTGAAAATGA TACGCAACCT ATGCTTCTAC AGAACTGCCC AGGGTCAGTC 
ACACACCGTT TACTAGAAGA AGACACACCA AGGTATATGC GTGCAACTGA TCCATACAGT 
CATCACTTAG GAAGATCAAA TGAAGAGGAG GAAGCTTCAG ATTCTTCTGT AGAGAAACAA 
CCCAGATCAT CCAGATACCG CACAGAAACC ACAGGAGGAA ATACAGAGTC TTTGTACAGT 
ACAGGAAACA TGGATACCCA TGAACTAGAG TCTAAAGCAG AAAGAATAGC TAGGTACAAG 
GCAGAGAGAC GGCGCCAACT GGCAGAAAAG TATGGATTAT CTCTTGATTC TGATTTGGAT 
TCTGACTACT CATCCAGATA TTCCCGGGCA AGAAAAGATC CTGACAGTGT GGAAAGAAAG 
GCATTGAGAA GTGAAAGGCA TGATGATGAA AACAAGGACT CTGGCTCCTT GTATTTGTCC 
AGGACTGAGG TGAAGGAGTC AAAGAGTGTG CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT 
GAAAAAGATG GTATTCCAGC TAAAGAAGAG CTTTCAAATG AAAAGAGTGA TAAAAGAGCA 
GATGATGACA GTGCCATTAG ACAGGCTTCT GACCCTTCAG CAACATTGGA TAGTTCTGTC 
TCACTAACAC TTTCTGGACG AGAGTCTTCC TCCTGTAATG AAGTGCCAAT ATCACCAAAG 
CAAAGCCCCC GAGAGTCTCT TTCTTCACCA AAGCGGGCAG CCTCACCAAT CCATTTGCAG 
AATGATCAGC CCTTGCACAG TAACATCAGA CAAAGTAGTG CAGGGAAAGC AAAACCTGAA 
TGGTTCCTTC AGAAAGATTC AGAAGGGGAC ACACCATCTC TTATCAGCTG GCCCTCCAGA 
GTAAAAGTTA GAGAGAAACT GGTGAAAGAG GAAAGTGCAC GTGGAAGCCC TGAACCTGTC 
ACAGACAGTG TATCTCAGAG AAAATCCCAG CCAGTGTCAG CCCACTACAT GCCTCTTCAG 
ACAGAAACGT CTGCCTTTGA TAGGGTTCTC AATCAGCCCG TCAGTGCAGT CCGCCAACCA 
ATACATGGGT ATATTCAGCC TGCTGGTGTT GCACACACGG CTCAGCTGAT GGCAACAGAA 
GAACCAGAAA GCATCTTCGT TGGCAGCCGC CTCCTTCCAG ACAGTGTCAG CCCCTTAACG 
AAATCAGCAG CAGCCACTGC ACCAGAGGGT GAAAAGAAAG AGACGCTGGC TTATGGAGCA 
AAGCCTGGCG AAGAAGAATC CCCAGAGGGT GAGAAGGCTT CCAAAGGCAG AAGACCAATT 
TTGCCATCTG AGATTCGCAA GCAAGGGAAG AGCCACGAAG GCCTCTTTGG AGGAGAAGAA 
CCAGATACCA GTGTGGGGAG CTCCTCATCT GCAAGCAAGC CGAAGGTTGG CTCAGATGTT 
CAGCAGGAGC AGGAATGCAA AAAGAAGCAG GCGCCTGAGC AGCAGCTGAA GACACCTGAG 
AACATGGAGA GGAGCGAAGC TGTCATGTAC ATACAGAGCG GGTCTGTATC AAAGCCAGCC 
CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA AAGCATAAAG TCCTGACTCG CTCCATGTCT 
GACTATACTG TGCCTCCTAA GCTAGAAGCT TTTCATAGTA AGAGCATGGA AGCAAACGCA 
GCAAATGGTG AGATTGGCAT GGTTGACACA AAGGTCTCTG TTGCCCAGCT CCGTAATGTC 
TTTCTGGAAA CTGCTCATGC CAACAAGAAA ACGGACCTCG AATCTCGGTT TGAGATGTCA 
ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT GACCGTGAAA GAGGGCCGCG AAGGCCGAGG 
CGCTATTTCA CTCCTGGTGA AAATAGAAAG ACTTCTGAGC GATTTAAAAC CCAACCTATA 
ACGTCAGCAG AACGAAAGGA GACGGATAGG TCTATTATGT GTGCTGAAAT ACCAACTGCT 
GACGATGAAG AAAAATTAGA TGACCGAGCC AAACTAAGTG TAGCTGCAAA GAGACTGCTT 
TTTAGAGAAA TGGAAAAATC TTTTGAAATG AAGAATATTC CTAAACCACC TACGAGAAGC 
TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG CAGGACAGAT CACACACGCA GCCTATTACC 
AGTGAGGAAG TGGTCATTGC AGCTACTGAA ACTACCCCTG CTTCACGTTC TGTGAATACC 
CACACAGTAG TGGCAAGAGT ACCTAGTCCC ACTCTAGTTA AGAGCACTGT ACAACCTACC 
AGCTTGCAGG CATCAGCACA CCAAAAAATA CTAGCTAAAG AGGAGATAAG AGCAGCCAAA 
GAGGCGATGG GGCAAGATCA ATCCGATGAA CCAGATTCTT CCACATTAAG CTTGGCAGAA 
AAGATGGCTT TGTTTAACAA ACTGTCTCAG CCAGTATCCA GGGCTATATC CACAAGGAGT 
CGGGGAGATA TTAGGCACAG AAGGATGAAT ACTCGTTACC AGACCCAGCC AGTCACTCTT 
GGAGAAGTTG AGCAGATGCA ATGGAAAAAG AGTAAACCGG TGCAAAACGA AAGTGGAAAA 
CTAACTCCCC TGTCAAGTAC AGTTTCAACA TCAGTTTCAG CAATGGCTTC TGCCCTTTCT 
GCACTGTTTG CTGGAGACAT GCATGCGAAG TCACGTAGTG ATGGAAGTGT GAGCGCTGCC 
ACAAAAGATG AGTTGAGATT CCATGCTTCA GCAGAAAGCT CAGACTCATC AGGAAAACGC 
ACACAGAAGT CAGAACAGTG GCAGCCCTCA ATGGAAGTGC TGGAAAGCAA AAGAGCATCA 
AAGAAGCATG AAGAAGAAGG AAAGAGGTTT CTAGCACATG AGGCTAATGA AATCAGAAAG 
TACAGCTCCT TTGAGGATGA AACCACACAT CCTGTTCTTG AAAAAACAGG AGACTACCAT 
AAAGAGGCAG CCTACAGCTT CCTGAGGAAG GGCAGCATGG AACTGTTTAG TTCACAACCA 
CTTTTTCAGC CTCTTGAAAG GAAGGAAATT GATACCATCA TGCAAGACCA CGTGGAGCCT 
ACCTCTGAAC TCACCAGCAC ACCAGCAACG AGGACACTTT CCCAGACTAC AGCTGCAGCA 
TCCTGTAGGC TACAGGAGCT GTCTGAACAA CTGGAGGGAA AGTTCTACAA GAATTCCTGT 
GAGATGTTTT CTGCTGGAGA GAACAAAATA CAGACAACTG AGGATGCCAC CGATAGCTCC 
AGCAAAACGA TGTCAATTAA AGAAAGGTTG GCATTGCTGA AGAAAAGTGG TGAAGAAGAT 
TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA TATGCAAAAG TGTCTGCCAC TGATCGTAGC 
GCACAGATGC AAGAAGTTGA GCAGCTGTTG AAAAAGGAAC CTGTATATGC TTCTACTTAC 
TCTCCTGTTA TACCTGCTGT CCATAAATTT CATCCTTTTC TACCTATTAA TCAGAGGATA 
GCAGATAATC AGGAGAGTCA AATGACTATA GAAGAAAGAA AACACCTCAT CACAGCTCGA 
GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC GCAGCAAATG ACTCCACGCA GTTCACCGTA 
GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT TCTCCAAGTG CACTAACACC AGTAGCATCC 
CCTTATGGTA ACAGGCAGAA GTCTACAACT CCGGTCACAA AGCCCTTGGA AGAAATTGAA 
GCTAGGCCTG ATATGCAATT GGAATCAGAT ATGAAGCTTG ACAAGCTGGA GTCATTCTTG 
GGAAGGCTGA ATAACAAAGT CGCTGGAATG CAAGAGACAG TGTTGACAGT TACAGGAAAA 
TCTGTGAAAG AGGTGATGAA GCTGGATGAT GATGAAACAT TTTCCAAATT TTACCGCCGT 
GTGGATTTCC CTTCCTCCTC TGTGCCTTTG GACCTTGATG AGGACTTTGA TGCCATCTTT 
GACCCTTATG CACCAAAGTT AATATCCTCT GTAGCAGAGC ACAAACGTGC AGTGAGGCCT 
ATGCGCAGGG TTCAGTCCTC CAGAAACCCC CTGAAAATGC TGGCAGCAAG AGAAGATATT 
CTTCATGAGT ACACTGAACA AAGACTGAAT GTTGCCTTCA TGGAGTCAAA GCGAATGAAA 
GTAGAAAAAA TGTCTGCAAA CTCAAATTTC TCAGAAGTAG CACTTGCTGG TTTGGCAAGC 
AAGGAGAACT TCAGCAACGT TAGTCTGCGG AGTGTTAACC TAACAGAGCA AAATTCTAAC 
AACAGTGCTG TGCCATACAA GAAGTTAATG CTGCTGCAAA TTAAAGGAAG AAGACATGTT 
CAAACAAGAT TAGTTGAACC AAGGGCCTCA TCACTGAACA GTGGAGACTG TTTCCTATTG 
TTAACTCCAC ATCTCTGTTT CTTATGGGTA GGAGAGTTTG CAAATGTCAT AGAAAAAGCA 
AAGGCTTCAG AACTTGCAAC TTTGATTCAG ACAAAGAGGG AACTTGGCTG TAGAGCTTCT 
TATATACAGA CTATAGAAGA AGGAATAAAC ACCCATACCC ATGCAGCCAA AGACTTCTGG 
AAACTCCTTG GTGGCCAAGC AAATTACCAG TCTGCTGGAA GACCTGAAGA GGATGAAATG 
TATGAAGCTG CCATAATAGA AACTAATTGC ATTTACCGTC TTGTAGAGGA TAAACTCATT 
CCTGAAGATG ACTACTGGGG AAAGATGCCA AAATGTACCC TGCTACAACC AAAAGAGGTG 
CTCGTATTTG ATTTTGGCAG TGAAGTGTAT GTCTGGCATG GAAAAGAAGT TACTTTAGCA 
CAAAGAAAAG TGGCATTCCA GCTGGCAAAA CACTTATGGA ATGGCACCTT CGACTACTCA 
AATTGTGACA TAAATCCTCT GGACCCAGGA GAATGCAATC CCCTCATACC CAGAAAGGGA 
CAAGGACGTC CAGACTGGGC TGTGTTTGGC AGGCTCACAG AACATAATGA GACCATACTG 
TTCAAAGAAA AATTCCTTGA TTGGACAGAG CTGAAGAAAC TCAATGAGAA GAATTCTAGT 
GAATCCCTTC ACCAGAAGGA AGAATCCAAA TCTGATTCTA AACCATATGA TGTCATGCTA 
ATGGTACCTG TGCCCCAAAC TGCTGTAGGC ACGGTTCTGG ATGGAATGAA TATTGGCCGG 
GGCTATGGAC TTGTAGAAGG AGAAGATGGG AGGCAGTTTG AAATTATCAC TGCATCTGTG 
GATGTCTGGC ACATCCTGGA GTTTGATTAT AGTAGATTGC CTAAACAAAG CATAGGACAG 
TTCCATGAGG GAGACACATA CGTGGTGAAG TGGAAGTACA TGGTGAGCAC TACAGTTGGA 
AGCAGACAAA AAGGAGAGCA ACAAGTAAGA GCTGTTGGAA AAGAGAAATG TGTGTATTTC 
TTTTGGCAAG GAAGGCACTC CACAGTGAGT GAGAAAGGGA CATCGGCACT GATGACAGTG 
GAACTTGATG AGGAGAGAGG AGCACAGGTA CAAGTGCTTC AAGGGAAAGA ACTACCATGC 
TTCCTGCAGT GCTTCCAAGG AGGGATGATT GTGCATGCTG GAAGAAGGGA AGAGGAAGAA 
GAAAATGCAC AAAGTGACTG GAGACTATAT TGCGTGCGAG GAGAAGTTCC CAATGAAGGA 
AACTTACTTG AAGTAGCATG TCACTGCAGC AGCCTGCGTT CCCGGACATC CATGATTGTC 
CTCAATATAA ATAAAGCTCT TATCTACCTG TGGCATGGCT GCAAAGCACA ATCTCACACC 
AAGGATGTAG GAAGAACAGC AGCCAATAAA ATAAAAGAAC AATGTCCGCT GGAAGCAGGG 
CTGCACAGCA GCAGCAAGGT GACAATACAC GAATGTGATG AAGGGTCAGA GCCTTTGGGA 
TTCTGGGATG CATTAGGAAG GCGAGATCGA AAGGCCTATG ATTGCATGTT GCAAGATCCG 
GGAAAGTTTA ATTTCACCCC CCGCCTCTTC AGCCTCAGTA GTTCTTCGGG AGAATTCTCA 
GCCACTGAGT ACGTTTACCC TTCAAGAGAC CCTACTGTCA TCAATTCTAT GCCCTTCTTG 
CAAGAGGATC TTTACACTGC CCCACAGCCA GCACTGTTCC TTGTTGACAA TCACCATGAA 
GTGTACCTGT GGCAAGGCTG GTGGCCTGTG GAAAACAAAA TCACTGGATC AGCTCGAATC 
AGGTGGGCTA CAGACAGGAA ATGCGCCATG GAGACCGTGC TCCAGTACTG CAAAGGAAAA 
AATGTAAAGA AGCCCCCAAA GTCCTATCTC ATTCATGCCG GCCTAGAGCC TCTGACCTTC 
ACTAATATGT TCCCAAGTTG GGAACACAGG GAAGACATTG CAGAGATCAC AGAAATGGAT 
GCTGACGTTT CTAATCAGAT AATCCTTGTA GAAGATGTGC TGGCCAAGCT GTGTAAAACT 
GTGTATCCCC TAGCTGATCT CTTAGCCAGG CCTTTACCTG AGGGAGTTGA TCCTCTGAAG 
CTTGAAATTT ATCTTTCAGA TGAGGACTTT GAGGTTGCAT TAGAGATGAC AAGAGAAGAG 
TACAATGCGC TGCCATCTTG GAAGCAGGTT AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148338.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004106.1 Gallus gallus supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OGa146728
Clone ID Related Accession (Same CDS sequence)	XM_025148338.1
Accession Version	XM_025148338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGAGGATAGC AGATAATCAG 
GAGAGTCAAA TGACTATAGA AGAAAGAAAA CACCTCATCA CAGCTCGAGA AGAAGCCTGG 
AAGTCCAGAG GTAAAGGCGC AGCAAATGAC TCCACGCAGT TCACCGTAGC TGGTCGAATG 
GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA CTAACACCAG TAGCATCCCC TTATGGTAAC 
AGGCAGAAGT CTACAACTCC GGTCACAAAG CCCTTGGAAG AAATTGAAGC TAGGCCTGAT 
ATGCAATTGG AATCAGATAT GAAGCTTGAC AAGCTGGAGT CATTCTTGGG AAGGCTGAAT 
AACAAAGTCG CTGGAATGCA AGAGACAGTG TTGACAGTTA CAGGAAAATC TGTGAAAGAG 
GTGATGAAGC TGGATGATGA TGAAACATTT TCCAAATTTT ACCGCCGTGT GGATTTCCCT 
TCCTCCTCTG TGCCTTTGGA CCTTGATGAG GACTTTGATG CCATCTTTGA CCCTTATGCA 
CCAAAGTTAA TATCCTCTGT AGCAGAGCAC AAACGTGCAG TGAGGCCTAT GCGCAGGGTT 
CAGTCCTCCA GAAACCCCCT GAAAATGCTG GCAGCAAGAG AAGATATTCT TCATGAGTAC 
ACTGAACAAA GACTGAATGT TGCCTTCATG GAGTCAAAGC GAATGAAAGT AGAAAAAATG 
TCTGCAAACT CAAATTTCTC AGAAGTAGCA CTTGCTGGTT TGGCAAGCAA GGAGAACTTC 
AGCAACGTTA GTCTGCGGAG TGTTAACCTA ACAGAGCAAA ATTCTAACAA CAGTGCTGTG 
CCATACAAGA AGTTAATGCT GCTGCAAATT AAAGGAAGAA GACATGTTCA AACAAGATTA 
GTTGAACCAA GGGCCTCATC ACTGAACAGT GGAGACTGTT TCCTATTGTT AACTCCACAT 
CTCTGTTTCT TATGGGTAGG AGAGTTTGCA AATGTCATAG AAAAAGCAAA GGCTTCAGAA 
CTTGCAACTT TGATTCAGAC AAAGAGGGAA CTTGGCTGTA GAGCTTCTTA TATACAGACT 
ATAGAAGAAG GAATAAACAC CCATACCCAT GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT 
GGCCAAGCAA ATTACCAGTC TGCTGGAAGA CCTGAAGAGG ATGAAATGTA TGAAGCTGCC 
ATAATAGAAA CTAATTGCAT TTACCGTCTT GTAGAGGATA AACTCATTCC TGAAGATGAC 
TACTGGGGAA AGATGCCAAA ATGTACCCTG CTACAACCAA AAGAGGTGCT CGTATTTGAT 
TTTGGCAGTG AAGTGTATGT CTGGCATGGA AAAGAAGTTA CTTTAGCACA AAGAAAAGTG 
GCATTCCAGC TGGCAAAACA CTTATGGAAT GGCACCTTCG ACTACTCAAA TTGTGACATA 
AATCCTCTGG ACCCAGGAGA ATGCAATCCC CTCATACCCA GAAAGGGACA AGGACGTCCA 
GACTGGGCTG TGTTTGGCAG GCTCACAGAA CATAATGAGA CCATACTGTT CAAAGAAAAA 
TTCCTTGATT GGACAGAGCT GAAGAAACTC AATGAGAAGA ATTCTAGTGA ATCCCTTCAC 
CAGAAGGAAG AATCCAAATC TGATTCTAAA CCATATGATG TCATGCTAAT GGTACCTGTG 
CCCCAAACTG CTGTAGGCAC GGTTCTGGAT GGAATGAATA TTGGCCGGGG CTATGGACTT 
GTAGAAGGAG AAGATGGGAG GCAGTTTGAA ATTATCACTG CATCTGTGGA TGTCTGGCAC 
ATCCTGGAGT TTGATTATAG TAGATTGCCT AAACAAAGCA TAGGACAGTT CCATGAGGGA 
GACACATACG TGGTGAAGTG GAAGTACATG GTGAGCACTA CAGTTGGAAG CAGACAAAAA 
GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA GAGAAATGTG TGTATTTCTT TTGGCAAGGA 
AGGCACTCCA CAGTGAGTGA GAAAGGGACA TCGGCACTGA TGACAGTGGA ACTTGATGAG 
GAGAGAGGAG CACAGGTACA AGTGCTTCAA GGGAAAGAAC TACCATGCTT CCTGCAGTGC 
TTCCAAGGAG GGATGATTGT GCATGCTGGA AGAAGGGAAG AGGAAGAAGA AAATGCACAA 
AGTGACTGGA GACTATATTG CGTGCGAGGA GAAGTTCCCA ATGAAGGAAA CTTACTTGAA 
GTAGCATGTC ACTGCAGCAG CCTGCGTTCC CGGACATCCA TGATTGTCCT CAATATAAAT 
AAAGCTCTTA TCTACCTGTG GCATGGCTGC AAAGCACAAT CTCACACCAA GGATGTAGGA 
AGAACAGCAG CCAATAAAAT AAAAGAACAA TGTCCGCTGG AAGCAGGGCT GCACAGCAGC 
AGCAAGGTGA CAATACACGA ATGTGATGAA GGGTCAGAGC CTTTGGGATT CTGGGATGCA 
TTAGGAAGGC GAGATCGAAA GGCCTATGAT TGCATGTTGC AAGATCCGGG AAAGTTTAAT 
TTCACCCCCC GCCTCTTCAG CCTCAGTAGT TCTTCGGGAG AATTCTCAGC CACTGAGTAC 
GTTTACCCTT CAAGAGACCC TACTGTCATC AATTCTATGC CCTTCTTGCA AGAGGATCTT 
TACACTGCCC CACAGCCAGC ACTGTTCCTT GTTGACAATC ACCATGAAGT GTACCTGTGG 
CAAGGCTGGT GGCCTGTGGA AAACAAAATC ACTGGATCAG CTCGAATCAG GTGGGCTACA 
GACAGGAAAT GCGCCATGGA GACCGTGCTC CAGTACTGCA AAGGAAAAAA TGTAAAGAAG 
CCCCCAAAGT CCTATCTCAT TCATGCCGGC CTAGAGCCTC TGACCTTCAC TAATATGTTC 
CCAAGTTGGG AACACAGGGA AGACATTGCA GAGATCACAG AAATGGATGC TGACGTTTCT 
AATCAGATAA TCCTTGTAGA AGATGTGCTG GCCAAGCTGT GTAAAACTGT GTATCCCCTA 
GCTGATCTCT TAGCCAGGCC TTTACCTGAG GGAGTTGATC CTCTGAAGCT TGAAATTTAT 
CTTTCAGATG AGGACTTTGA GGTTGCATTA GAGATGACAA GAGAAGAGTA CAATGCGCTG 
CCATCTTGGA AGCAGGTTAA TCTGAAAAAG GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004106.1
CDS	19..6906
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148338.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148338.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGAGGATAGC AGATAATCAG 
GAGAGTCAAA TGACTATAGA AGAAAGAAAA CACCTCATCA CAGCTCGAGA AGAAGCCTGG 
AAGTCCAGAG GTAAAGGCGC AGCAAATGAC TCCACGCAGT TCACCGTAGC TGGTCGAATG 
GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA CTAACACCAG TAGCATCCCC TTATGGTAAC 
AGGCAGAAGT CTACAACTCC GGTCACAAAG CCCTTGGAAG AAATTGAAGC TAGGCCTGAT 
ATGCAATTGG AATCAGATAT GAAGCTTGAC AAGCTGGAGT CATTCTTGGG AAGGCTGAAT 
AACAAAGTCG CTGGAATGCA AGAGACAGTG TTGACAGTTA CAGGAAAATC TGTGAAAGAG 
GTGATGAAGC TGGATGATGA TGAAACATTT TCCAAATTTT ACCGCCGTGT GGATTTCCCT 
TCCTCCTCTG TGCCTTTGGA CCTTGATGAG GACTTTGATG CCATCTTTGA CCCTTATGCA 
CCAAAGTTAA TATCCTCTGT AGCAGAGCAC AAACGTGCAG TGAGGCCTAT GCGCAGGGTT 
CAGTCCTCCA GAAACCCCCT GAAAATGCTG GCAGCAAGAG AAGATATTCT TCATGAGTAC 
ACTGAACAAA GACTGAATGT TGCCTTCATG GAGTCAAAGC GAATGAAAGT AGAAAAAATG 
TCTGCAAACT CAAATTTCTC AGAAGTAGCA CTTGCTGGTT TGGCAAGCAA GGAGAACTTC 
AGCAACGTTA GTCTGCGGAG TGTTAACCTA ACAGAGCAAA ATTCTAACAA CAGTGCTGTG 
CCATACAAGA AGTTAATGCT GCTGCAAATT AAAGGAAGAA GACATGTTCA AACAAGATTA 
GTTGAACCAA GGGCCTCATC ACTGAACAGT GGAGACTGTT TCCTATTGTT AACTCCACAT 
CTCTGTTTCT TATGGGTAGG AGAGTTTGCA AATGTCATAG AAAAAGCAAA GGCTTCAGAA 
CTTGCAACTT TGATTCAGAC AAAGAGGGAA CTTGGCTGTA GAGCTTCTTA TATACAGACT 
ATAGAAGAAG GAATAAACAC CCATACCCAT GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT 
GGCCAAGCAA ATTACCAGTC TGCTGGAAGA CCTGAAGAGG ATGAAATGTA TGAAGCTGCC 
ATAATAGAAA CTAATTGCAT TTACCGTCTT GTAGAGGATA AACTCATTCC TGAAGATGAC 
TACTGGGGAA AGATGCCAAA ATGTACCCTG CTACAACCAA AAGAGGTGCT CGTATTTGAT 
TTTGGCAGTG AAGTGTATGT CTGGCATGGA AAAGAAGTTA CTTTAGCACA AAGAAAAGTG 
GCATTCCAGC TGGCAAAACA CTTATGGAAT GGCACCTTCG ACTACTCAAA TTGTGACATA 
AATCCTCTGG ACCCAGGAGA ATGCAATCCC CTCATACCCA GAAAGGGACA AGGACGTCCA 
GACTGGGCTG TGTTTGGCAG GCTCACAGAA CATAATGAGA CCATACTGTT CAAAGAAAAA 
TTCCTTGATT GGACAGAGCT GAAGAAACTC AATGAGAAGA ATTCTAGTGA ATCCCTTCAC 
CAGAAGGAAG AATCCAAATC TGATTCTAAA CCATATGATG TCATGCTAAT GGTACCTGTG 
CCCCAAACTG CTGTAGGCAC GGTTCTGGAT GGAATGAATA TTGGCCGGGG CTATGGACTT 
GTAGAAGGAG AAGATGGGAG GCAGTTTGAA ATTATCACTG CATCTGTGGA TGTCTGGCAC 
ATCCTGGAGT TTGATTATAG TAGATTGCCT AAACAAAGCA TAGGACAGTT CCATGAGGGA 
GACACATACG TGGTGAAGTG GAAGTACATG GTGAGCACTA CAGTTGGAAG CAGACAAAAA 
GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA GAGAAATGTG TGTATTTCTT TTGGCAAGGA 
AGGCACTCCA CAGTGAGTGA GAAAGGGACA TCGGCACTGA TGACAGTGGA ACTTGATGAG 
GAGAGAGGAG CACAGGTACA AGTGCTTCAA GGGAAAGAAC TACCATGCTT CCTGCAGTGC 
TTCCAAGGAG GGATGATTGT GCATGCTGGA AGAAGGGAAG AGGAAGAAGA AAATGCACAA 
AGTGACTGGA GACTATATTG CGTGCGAGGA GAAGTTCCCA ATGAAGGAAA CTTACTTGAA 
GTAGCATGTC ACTGCAGCAG CCTGCGTTCC CGGACATCCA TGATTGTCCT CAATATAAAT 
AAAGCTCTTA TCTACCTGTG GCATGGCTGC AAAGCACAAT CTCACACCAA GGATGTAGGA 
AGAACAGCAG CCAATAAAAT AAAAGAACAA TGTCCGCTGG AAGCAGGGCT GCACAGCAGC 
AGCAAGGTGA CAATACACGA ATGTGATGAA GGGTCAGAGC CTTTGGGATT CTGGGATGCA 
TTAGGAAGGC GAGATCGAAA GGCCTATGAT TGCATGTTGC AAGATCCGGG AAAGTTTAAT 
TTCACCCCCC GCCTCTTCAG CCTCAGTAGT TCTTCGGGAG AATTCTCAGC CACTGAGTAC 
GTTTACCCTT CAAGAGACCC TACTGTCATC AATTCTATGC CCTTCTTGCA AGAGGATCTT 
TACACTGCCC CACAGCCAGC ACTGTTCCTT GTTGACAATC ACCATGAAGT GTACCTGTGG 
CAAGGCTGGT GGCCTGTGGA AAACAAAATC ACTGGATCAG CTCGAATCAG GTGGGCTACA 
GACAGGAAAT GCGCCATGGA GACCGTGCTC CAGTACTGCA AAGGAAAAAA TGTAAAGAAG 
CCCCCAAAGT CCTATCTCAT TCATGCCGGC CTAGAGCCTC TGACCTTCAC TAATATGTTC 
CCAAGTTGGG AACACAGGGA AGACATTGCA GAGATCACAG AAATGGATGC TGACGTTTCT 
AATCAGATAA TCCTTGTAGA AGATGTGCTG GCCAAGCTGT GTAAAACTGT GTATCCCCTA 
GCTGATCTCT TAGCCAGGCC TTTACCTGAG GGAGTTGATC CTCTGAAGCT TGAAATTTAT 
CTTTCAGATG AGGACTTTGA GGTTGCATTA GAGATGACAA GAGAAGAGTA CAATGCGCTG 
CCATCTTGGA AGCAGGTTAA TCTGAAAAAG GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148339.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004107.1 Gallus gallus supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OGa146729
Clone ID Related Accession (Same CDS sequence)	XM_025148339.1
Accession Version	XM_025148339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGAGT AAAAGTTAGA 
GAGAAACTGG TGAAAGAGGA AAGTGCACGT GGAAGCCCTG AACCTGTCAC AGACAGTGTA 
TCTCAGAGAA AATCCCAGCC AGTGTCAGCC CACTACATGC CTCTTCAGAC AGAAACGTCT 
GCCTTTGATA GGGTTCTCAA TCAGCCCGTC AGTGCAGTCC GCCAACCAAT ACATGGGTAT 
ATTCAGCCTG CTGGTGTTGC ACACACGGCT CAGCTGATGG CAACAGAAGA ACCAGAAAGC 
ATCTTCGTTG GCAGCCGCCT CCTTCCAGAC AGTGTCAGCC CCTTAACGAA ATCAGCAGCA 
GCCACTGCAC CAGAGGGTGA AAAGAAAGAG ACGCTGGCTT ATGGAGCAAA GCCTGGCGAA 
GAAGAATCCC CAGAGGGTGA GAAGGCTTCC AAAGGCAGAA GACCAATTTT GCCATCTGAG 
ATTCGCAAGC AAGGGAAGAG CCACGAAGGC CTCTTTGGAG GAGAAGAACC AGATACCAGT 
GTGGGGAGCT CCTCATCTGC AAGCAAGCCG AAGGTTGGCT CAGATGTTCA GCAGGAGCAG 
GAATGCAAAA AGAAGCAGGC GCCTGAGCAG CAGCTGAAGA CACCTGAGAA CATGGAGAGG 
AGCGAAGCTG TCATGTACAT ACAGAGCGGG TCTGTATCAA AGCCAGCCCA GGCAAAGGGC 
CCCGTTTGGA AGGTGTCAAA GCATAAAGTC CTGACTCGCT CCATGTCTGA CTATACTGTG 
CCTCCTAAGC TAGAAGCTTT TCATAGTAAG AGCATGGAAG CAAACGCAGC AAATGGTGAG 
ATTGGCATGG TTGACACAAA GGTCTCTGTT GCCCAGCTCC GTAATGTCTT TCTGGAAACT 
GCTCATGCCA ACAAGAAAAC GGACCTCGAA TCTCGGTTTG AGATGTCAAC GTCAGGCAGC 
GCTTTGTCTG CCGGTGGTGA CCGTGAAAGA GGGCCGCGAA GGCCGAGGCG CTATTTCACT 
CCTGGTGAAA ATAGAAAGAC TTCTGAGCGA TTTAAAACCC AACCTATAAC GTCAGCAGAA 
CGAAAGGAGA CGGATAGGTC TATTATGTGT GCTGAAATAC CAACTGCTGA CGATGAAGAA 
AAATTAGATG ACCGAGCCAA ACTAAGTGTA GCTGCAAAGA GACTGCTTTT TAGAGAAATG 
GAAAAATCTT TTGAAATGAA GAATATTCCT AAACCACCTA CGAGAAGCTC AGCAGTAGAG 
AGAAGAATGC GGCGTTTGCA GGACAGATCA CACACGCAGC CTATTACCAG TGAGGAAGTG 
GTCATTGCAG CTACTGAAAC TACCCCTGCT TCACGTTCTG TGAATACCCA CACAGTAGTG 
GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG AGCACTGTAC AACCTACCAG CTTGCAGGCA 
TCAGCACACC AAAAAATACT AGCTAAAGAG GAGATAAGAG CAGCCAAAGA GGCGATGGGG 
CAAGATCAAT CCGATGAACC AGATTCTTCC ACATTAAGCT TGGCAGAAAA GATGGCTTTG 
TTTAACAAAC TGTCTCAGCC AGTATCCAGG GCTATATCCA CAAGGAGTCG GGGAGATATT 
AGGCACAGAA GGATGAATAC TCGTTACCAG ACCCAGCCAG TCACTCTTGG AGAAGTTGAG 
CAGATGCAAT GGAAAAAGAG TAAACCGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGAGGATAGC AGATAATCAG 
GAGAGTCAAA TGACTATAGA AGAAAGAAAA CACCTCATCA CAGCTCGAGA AGAAGCCTGG 
AAGTCCAGAG GTAAAGGCGC AGCAAATGAC TCCACGCAGT TCACCGTAGC TGGTCGAATG 
GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA CTAACACCAG TAGCATCCCC TTATGGTAAC 
AGGCAGAAGT CTACAACTCC GGTCACAAAG CCCTTGGAAG AAATTGAAGC TAGGCCTGAT 
ATGCAATTGG AATCAGATAT GAAGCTTGAC AAGCTGGAGT CATTCTTGGG AAGGCTGAAT 
AACAAAGTCG CTGGAATGCA AGAGACAGTG TTGACAGTTA CAGGAAAATC TGTGAAAGAG 
GTGATGAAGC TGGATGATGA TGAAACATTT TCCAAATTTT ACCGCCGTGT GGATTTCCCT 
TCCTCCTCTG TGCCTTTGGA CCTTGATGAG GACTTTGATG CCATCTTTGA CCCTTATGCA 
CCAAAGTTAA TATCCTCTGT AGCAGAGCAC AAACGTGCAG TGAGGCCTAT GCGCAGGGTT 
CAGTCCTCCA GAAACCCCCT GAAAATGCTG GCAGCAAGAG AAGATATTCT TCATGAGTAC 
ACTGAACAAA GACTGAATGT TGCCTTCATG GAGTCAAAGC GAATGAAAGT AGAAAAAATG 
TCTGCAAACT CAAATTTCTC AGAAGTAGCA CTTGCTGGTT TGGCAAGCAA GGAGAACTTC 
AGCAACGTTA GTCTGCGGAG TGTTAACCTA ACAGAGCAAA ATTCTAACAA CAGTGCTGTG 
CCATACAAGA AGTTAATGCT GCTGCAAATT AAAGGAAGAA GACATGTTCA AACAAGATTA 
GTTGAACCAA GGGCCTCATC ACTGAACAGT GGAGACTGTT TCCTATTGTT AACTCCACAT 
CTCTGTTTCT TATGGGTAGG AGAGTTTGCA AATGTCATAG AAAAAGCAAA GGCTTCAGAA 
CTTGCAACTT TGATTCAGAC AAAGAGGGAA CTTGGCTGTA GAGCTTCTTA TATACAGACT 
ATAGAAGAAG GAATAAACAC CCATACCCAT GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT 
GGCCAAGCAA ATTACCAGTC TGCTGGAAGA CCTGAAGAGG ATGAAATGTA TGAAGCTGCC 
ATAATAGAAA CTAATTGCAT TTACCGTCTT GTAGAGGATA AACTCATTCC TGAAGATGAC 
TACTGGGGAA AGATGCCAAA ATGTACCCTG CTACAACCAA AAGAGGTGCT CGTATTTGAT 
TTTGGCAGTG AAGTGTATGT CTGGCATGGA AAAGAAGTTA CTTTAGCACA AAGAAAAGTG 
GCATTCCAGC TGGCAAAACA CTTATGGAAT GGCACCTTCG ACTACTCAAA TTGTGACATA 
AATCCTCTGG ACCCAGGAGA ATGCAATCCC CTCATACCCA GAAAGGGACA AGGACGTCCA 
GACTGGGCTG TGTTTGGCAG GCTCACAGAA CATAATGAGA CCATACTGTT CAAAGAAAAA 
TTCCTTGATT GGACAGAGCT GAAGAAACTC AATGAGAAGA ATTCTAGTGA ATCCCTTCAC 
CAGAAGGAAG AATCCAAATC TGATTCTAAA CCATATGATG TCATGCTAAT GGTACCTGTG 
CCCCAAACTG CTGTAGGCAC GGTTCTGGAT GGAATGAATA TTGGCCGGGG CTATGGACTT 
GTAGAAGGAG AAGATGGGAG GCAGTTTGAA ATTATCACTG CATCTGTGGA TGTCTGGCAC 
ATCCTGGAGT TTGATTATAG TAGATTGCCT AAACAAAGCA TAGGACAGTT CCATGAGGGA 
GACACATACG TGGTGAAGTG GAAGTACATG GTGAGCACTA CAGTTGGAAG CAGACAAAAA 
GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA GAGAAATGTG TGTATTTCTT TTGGCAAGGA 
AGGCACTCCA CAGTGAGTGA GAAAGGGACA TCGGCACTGA TGACAGTGGA ACTTGATGAG 
GAGAGAGGAG CACAGGTACA AGTGCTTCAA GGGAAAGAAC TACCATGCTT CCTGCAGTGC 
TTCCAAGGAG GGATGATTGT GCATGCTGGA AGAAGGGAAG AGGAAGAAGA AAATGCACAA 
AGTGACTGGA GACTATATTG CGTGCGAGGA GAAGTTCCCA ATGAAGGAAA CTTACTTGAA 
GTAGCATGTC ACTGCAGCAG CCTGCGTTCC CGGACATCCA TGATTGTCCT CAATATAAAT 
AAAGCTCTTA TCTACCTGTG GCATGGCTGC AAAGCACAAT CTCACACCAA GGATGTAGGA 
AGAACAGCAG CCAATAAAAT AAAAGAACAA TGTCCGCTGG AAGCAGGGCT GCACAGCAGC 
AGCAAGGTGA CAATACACGA ATGTGATGAA GGGTCAGAGC CTTTGGGATT CTGGGATGCA 
TTAGGAAGGC GAGATCGAAA GGCCTATGAT TGCATGTTGC AAGATCCGGG AAAGTTTAAT 
TTCACCCCCC GCCTCTTCAG CCTCAGTAGT TCTTCGGGAG AATTCTCAGC CACTGAGTAC 
GTTTACCCTT CAAGAGACCC TACTGTCATC AATTCTATGC CCTTCTTGCA AGAGGATCTT 
TACACTGCCC CACAGCCAGC ACTGTTCCTT GTTGACAATC ACCATGAAGT GTACCTGTGG 
CAAGGCTGGT GGCCTGTGGA AAACAAAATC ACTGGATCAG CTCGAATCAG GTGGGCTACA 
GACAGGAAAT GCGCCATGGA GACCGTGCTC CAGTACTGCA AAGGAAAAAA TGTAAAGAAG 
CCCCCAAAGT CCTATCTCAT TCATGCCGGC CTAGAGCCTC TGACCTTCAC TAATATGTTC 
CCAAGTTGGG AACACAGGGA AGACATTGCA GAGATCACAG AAATGGATGC TGACGTTTCT 
AATCAGATAA TCCTTGTAGA AGATGTGCTG GCCAAGCTGT GTAAAACTGT GTATCCCCTA 
GCTGATCTCT TAGCCAGGCC TTTACCTGAG GGAGTTGATC CTCTGAAGCT TGAAATTTAT 
CTTTCAGATG AGGACTTTGA GGTTGCATTA GAGATGACAA GAGAAGAGTA CAATGCGCTG 
CCATCTTGGA AGCAGGTTAA TCTGAAAAAG GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004107.1
CDS	19..6846
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148339.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148339.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGAGT AAAAGTTAGA 
GAGAAACTGG TGAAAGAGGA AAGTGCACGT GGAAGCCCTG AACCTGTCAC AGACAGTGTA 
TCTCAGAGAA AATCCCAGCC AGTGTCAGCC CACTACATGC CTCTTCAGAC AGAAACGTCT 
GCCTTTGATA GGGTTCTCAA TCAGCCCGTC AGTGCAGTCC GCCAACCAAT ACATGGGTAT 
ATTCAGCCTG CTGGTGTTGC ACACACGGCT CAGCTGATGG CAACAGAAGA ACCAGAAAGC 
ATCTTCGTTG GCAGCCGCCT CCTTCCAGAC AGTGTCAGCC CCTTAACGAA ATCAGCAGCA 
GCCACTGCAC CAGAGGGTGA AAAGAAAGAG ACGCTGGCTT ATGGAGCAAA GCCTGGCGAA 
GAAGAATCCC CAGAGGGTGA GAAGGCTTCC AAAGGCAGAA GACCAATTTT GCCATCTGAG 
ATTCGCAAGC AAGGGAAGAG CCACGAAGGC CTCTTTGGAG GAGAAGAACC AGATACCAGT 
GTGGGGAGCT CCTCATCTGC AAGCAAGCCG AAGGTTGGCT CAGATGTTCA GCAGGAGCAG 
GAATGCAAAA AGAAGCAGGC GCCTGAGCAG CAGCTGAAGA CACCTGAGAA CATGGAGAGG 
AGCGAAGCTG TCATGTACAT ACAGAGCGGG TCTGTATCAA AGCCAGCCCA GGCAAAGGGC 
CCCGTTTGGA AGGTGTCAAA GCATAAAGTC CTGACTCGCT CCATGTCTGA CTATACTGTG 
CCTCCTAAGC TAGAAGCTTT TCATAGTAAG AGCATGGAAG CAAACGCAGC AAATGGTGAG 
ATTGGCATGG TTGACACAAA GGTCTCTGTT GCCCAGCTCC GTAATGTCTT TCTGGAAACT 
GCTCATGCCA ACAAGAAAAC GGACCTCGAA TCTCGGTTTG AGATGTCAAC GTCAGGCAGC 
GCTTTGTCTG CCGGTGGTGA CCGTGAAAGA GGGCCGCGAA GGCCGAGGCG CTATTTCACT 
CCTGGTGAAA ATAGAAAGAC TTCTGAGCGA TTTAAAACCC AACCTATAAC GTCAGCAGAA 
CGAAAGGAGA CGGATAGGTC TATTATGTGT GCTGAAATAC CAACTGCTGA CGATGAAGAA 
AAATTAGATG ACCGAGCCAA ACTAAGTGTA GCTGCAAAGA GACTGCTTTT TAGAGAAATG 
GAAAAATCTT TTGAAATGAA GAATATTCCT AAACCACCTA CGAGAAGCTC AGCAGTAGAG 
AGAAGAATGC GGCGTTTGCA GGACAGATCA CACACGCAGC CTATTACCAG TGAGGAAGTG 
GTCATTGCAG CTACTGAAAC TACCCCTGCT TCACGTTCTG TGAATACCCA CACAGTAGTG 
GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG AGCACTGTAC AACCTACCAG CTTGCAGGCA 
TCAGCACACC AAAAAATACT AGCTAAAGAG GAGATAAGAG CAGCCAAAGA GGCGATGGGG 
CAAGATCAAT CCGATGAACC AGATTCTTCC ACATTAAGCT TGGCAGAAAA GATGGCTTTG 
TTTAACAAAC TGTCTCAGCC AGTATCCAGG GCTATATCCA CAAGGAGTCG GGGAGATATT 
AGGCACAGAA GGATGAATAC TCGTTACCAG ACCCAGCCAG TCACTCTTGG AGAAGTTGAG 
CAGATGCAAT GGAAAAAGAG TAAACCGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGAGGATAGC AGATAATCAG 
GAGAGTCAAA TGACTATAGA AGAAAGAAAA CACCTCATCA CAGCTCGAGA AGAAGCCTGG 
AAGTCCAGAG GTAAAGGCGC AGCAAATGAC TCCACGCAGT TCACCGTAGC TGGTCGAATG 
GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA CTAACACCAG TAGCATCCCC TTATGGTAAC 
AGGCAGAAGT CTACAACTCC GGTCACAAAG CCCTTGGAAG AAATTGAAGC TAGGCCTGAT 
ATGCAATTGG AATCAGATAT GAAGCTTGAC AAGCTGGAGT CATTCTTGGG AAGGCTGAAT 
AACAAAGTCG CTGGAATGCA AGAGACAGTG TTGACAGTTA CAGGAAAATC TGTGAAAGAG 
GTGATGAAGC TGGATGATGA TGAAACATTT TCCAAATTTT ACCGCCGTGT GGATTTCCCT 
TCCTCCTCTG TGCCTTTGGA CCTTGATGAG GACTTTGATG CCATCTTTGA CCCTTATGCA 
CCAAAGTTAA TATCCTCTGT AGCAGAGCAC AAACGTGCAG TGAGGCCTAT GCGCAGGGTT 
CAGTCCTCCA GAAACCCCCT GAAAATGCTG GCAGCAAGAG AAGATATTCT TCATGAGTAC 
ACTGAACAAA GACTGAATGT TGCCTTCATG GAGTCAAAGC GAATGAAAGT AGAAAAAATG 
TCTGCAAACT CAAATTTCTC AGAAGTAGCA CTTGCTGGTT TGGCAAGCAA GGAGAACTTC 
AGCAACGTTA GTCTGCGGAG TGTTAACCTA ACAGAGCAAA ATTCTAACAA CAGTGCTGTG 
CCATACAAGA AGTTAATGCT GCTGCAAATT AAAGGAAGAA GACATGTTCA AACAAGATTA 
GTTGAACCAA GGGCCTCATC ACTGAACAGT GGAGACTGTT TCCTATTGTT AACTCCACAT 
CTCTGTTTCT TATGGGTAGG AGAGTTTGCA AATGTCATAG AAAAAGCAAA GGCTTCAGAA 
CTTGCAACTT TGATTCAGAC AAAGAGGGAA CTTGGCTGTA GAGCTTCTTA TATACAGACT 
ATAGAAGAAG GAATAAACAC CCATACCCAT GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT 
GGCCAAGCAA ATTACCAGTC TGCTGGAAGA CCTGAAGAGG ATGAAATGTA TGAAGCTGCC 
ATAATAGAAA CTAATTGCAT TTACCGTCTT GTAGAGGATA AACTCATTCC TGAAGATGAC 
TACTGGGGAA AGATGCCAAA ATGTACCCTG CTACAACCAA AAGAGGTGCT CGTATTTGAT 
TTTGGCAGTG AAGTGTATGT CTGGCATGGA AAAGAAGTTA CTTTAGCACA AAGAAAAGTG 
GCATTCCAGC TGGCAAAACA CTTATGGAAT GGCACCTTCG ACTACTCAAA TTGTGACATA 
AATCCTCTGG ACCCAGGAGA ATGCAATCCC CTCATACCCA GAAAGGGACA AGGACGTCCA 
GACTGGGCTG TGTTTGGCAG GCTCACAGAA CATAATGAGA CCATACTGTT CAAAGAAAAA 
TTCCTTGATT GGACAGAGCT GAAGAAACTC AATGAGAAGA ATTCTAGTGA ATCCCTTCAC 
CAGAAGGAAG AATCCAAATC TGATTCTAAA CCATATGATG TCATGCTAAT GGTACCTGTG 
CCCCAAACTG CTGTAGGCAC GGTTCTGGAT GGAATGAATA TTGGCCGGGG CTATGGACTT 
GTAGAAGGAG AAGATGGGAG GCAGTTTGAA ATTATCACTG CATCTGTGGA TGTCTGGCAC 
ATCCTGGAGT TTGATTATAG TAGATTGCCT AAACAAAGCA TAGGACAGTT CCATGAGGGA 
GACACATACG TGGTGAAGTG GAAGTACATG GTGAGCACTA CAGTTGGAAG CAGACAAAAA 
GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA GAGAAATGTG TGTATTTCTT TTGGCAAGGA 
AGGCACTCCA CAGTGAGTGA GAAAGGGACA TCGGCACTGA TGACAGTGGA ACTTGATGAG 
GAGAGAGGAG CACAGGTACA AGTGCTTCAA GGGAAAGAAC TACCATGCTT CCTGCAGTGC 
TTCCAAGGAG GGATGATTGT GCATGCTGGA AGAAGGGAAG AGGAAGAAGA AAATGCACAA 
AGTGACTGGA GACTATATTG CGTGCGAGGA GAAGTTCCCA ATGAAGGAAA CTTACTTGAA 
GTAGCATGTC ACTGCAGCAG CCTGCGTTCC CGGACATCCA TGATTGTCCT CAATATAAAT 
AAAGCTCTTA TCTACCTGTG GCATGGCTGC AAAGCACAAT CTCACACCAA GGATGTAGGA 
AGAACAGCAG CCAATAAAAT AAAAGAACAA TGTCCGCTGG AAGCAGGGCT GCACAGCAGC 
AGCAAGGTGA CAATACACGA ATGTGATGAA GGGTCAGAGC CTTTGGGATT CTGGGATGCA 
TTAGGAAGGC GAGATCGAAA GGCCTATGAT TGCATGTTGC AAGATCCGGG AAAGTTTAAT 
TTCACCCCCC GCCTCTTCAG CCTCAGTAGT TCTTCGGGAG AATTCTCAGC CACTGAGTAC 
GTTTACCCTT CAAGAGACCC TACTGTCATC AATTCTATGC CCTTCTTGCA AGAGGATCTT 
TACACTGCCC CACAGCCAGC ACTGTTCCTT GTTGACAATC ACCATGAAGT GTACCTGTGG 
CAAGGCTGGT GGCCTGTGGA AAACAAAATC ACTGGATCAG CTCGAATCAG GTGGGCTACA 
GACAGGAAAT GCGCCATGGA GACCGTGCTC CAGTACTGCA AAGGAAAAAA TGTAAAGAAG 
CCCCCAAAGT CCTATCTCAT TCATGCCGGC CTAGAGCCTC TGACCTTCAC TAATATGTTC 
CCAAGTTGGG AACACAGGGA AGACATTGCA GAGATCACAG AAATGGATGC TGACGTTTCT 
AATCAGATAA TCCTTGTAGA AGATGTGCTG GCCAAGCTGT GTAAAACTGT GTATCCCCTA 
GCTGATCTCT TAGCCAGGCC TTTACCTGAG GGAGTTGATC CTCTGAAGCT TGAAATTTAT 
CTTTCAGATG AGGACTTTGA GGTTGCATTA GAGATGACAA GAGAAGAGTA CAATGCGCTG 
CCATCTTGGA AGCAGGTTAA TCTGAAAAAG GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148340.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004108.1 Gallus gallus supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OGa146730
Clone ID Related Accession (Same CDS sequence)	XM_025148340.1
Accession Version	XM_025148340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CTGAAACTAC CCCTGCTTCA 
CGTTCTGTGA ATACCCACAC AGTAGTGGCA AGAGTACCTA GTCCCACTCT AGTTAAGAGC 
ACTGTACAAC CTACCAGCTT GCAGGCATCA GCACACCAAA AAATACTAGC TAAAGAGGAG 
ATAAGAGCAG CCAAAGAGGC GATGGGGCAA GATCAATCCG ATGAACCAGA TTCTTCCACA 
TTAAGCTTGG CAGAAAAGAT GGCTTTGTTT AACAAACTGT CTCAGCCAGT ATCCAGGGCT 
ATATCCACAA GGAGTCGGGG AGATATTAGG CACAGAAGGA TGAATACTCG TTACCAGACC 
CAGCCAGTCA CTCTTGGAGA AGTTGAGCAG ATGCAATGGA AAAAGAGTAA ACCGGTGCAA 
AACGAAAGTG GAAAACTAAC TCCCCTGTCA AGTACAGTTT CAACATCAGT TTCAGCAATG 
GCTTCTGCCC TTTCTGCACT GTTTGCTGGA GACATGCATG CGAAGTCACG TAGTGATGGA 
AGTGTGAGCG CTGCCACAAA AGATGAGTTG AGATTCCATG CTTCAGCAGA AAGCTCAGAC 
TCATCAGGAA AACGCACACA GAAGTCAGAA CAGTGGCAGC CCTCAATGGA AGTGCTGGAA 
AGCAAAAGAG CATCAAAGAA GCATGAAGAA GAAGGAAAGA GGTTTCTAGC ACATGAGGCT 
AATGAAATCA GAAAGTACAG CTCCTTTGAG GATGAAACCA CACATCCTGT TCTTGAAAAA 
ACAGGAGACT ACCATAAAGA GGCAGCCTAC AGCTTCCTGA GGAAGGGCAG CATGGAACTG 
TTTAGTTCAC AACCACTTTT TCAGCCTCTT GAAAGGAAGG AAATTGATAC CATCATGCAA 
GACCACGTGG AGCCTACCTC TGAACTCACC AGCACACCAG CAACGAGGAC ACTTTCCCAG 
ACTACAGCTG CAGCATCCTG TAGGCTACAG GAGCTGTCTG AACAACTGGA GGGAAAGTTC 
TACAAGAATT CCTGTGAGAT GTTTTCTGCT GGAGAGAACA AAATACAGAC AACTGAGGAT 
GCCACCGATA GCTCCAGCAA AACGATGTCA ATTAAAGAAA GGTTGGCATT GCTGAAGAAA 
AGTGGTGAAG AAGATTGGAA GAGCAGGCTC AGTAAAAAGC AGGAATATGC AAAAGTGTCT 
GCCACTGATC GTAGCGCACA GATGCAAGAA GTTGAGCAGC TGTTGAAAAA GGAACCTGTA 
TATGCTTCTA CTTACTCTCC TGTTATACCT GCTGTCCATA AATTTCATCC TTTTCTACCT 
ATTAATCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004108.1
CDS	19..6693
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148340.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148340.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CTGAAACTAC CCCTGCTTCA 
CGTTCTGTGA ATACCCACAC AGTAGTGGCA AGAGTACCTA GTCCCACTCT AGTTAAGAGC 
ACTGTACAAC CTACCAGCTT GCAGGCATCA GCACACCAAA AAATACTAGC TAAAGAGGAG 
ATAAGAGCAG CCAAAGAGGC GATGGGGCAA GATCAATCCG ATGAACCAGA TTCTTCCACA 
TTAAGCTTGG CAGAAAAGAT GGCTTTGTTT AACAAACTGT CTCAGCCAGT ATCCAGGGCT 
ATATCCACAA GGAGTCGGGG AGATATTAGG CACAGAAGGA TGAATACTCG TTACCAGACC 
CAGCCAGTCA CTCTTGGAGA AGTTGAGCAG ATGCAATGGA AAAAGAGTAA ACCGGTGCAA 
AACGAAAGTG GAAAACTAAC TCCCCTGTCA AGTACAGTTT CAACATCAGT TTCAGCAATG 
GCTTCTGCCC TTTCTGCACT GTTTGCTGGA GACATGCATG CGAAGTCACG TAGTGATGGA 
AGTGTGAGCG CTGCCACAAA AGATGAGTTG AGATTCCATG CTTCAGCAGA AAGCTCAGAC 
TCATCAGGAA AACGCACACA GAAGTCAGAA CAGTGGCAGC CCTCAATGGA AGTGCTGGAA 
AGCAAAAGAG CATCAAAGAA GCATGAAGAA GAAGGAAAGA GGTTTCTAGC ACATGAGGCT 
AATGAAATCA GAAAGTACAG CTCCTTTGAG GATGAAACCA CACATCCTGT TCTTGAAAAA 
ACAGGAGACT ACCATAAAGA GGCAGCCTAC AGCTTCCTGA GGAAGGGCAG CATGGAACTG 
TTTAGTTCAC AACCACTTTT TCAGCCTCTT GAAAGGAAGG AAATTGATAC CATCATGCAA 
GACCACGTGG AGCCTACCTC TGAACTCACC AGCACACCAG CAACGAGGAC ACTTTCCCAG 
ACTACAGCTG CAGCATCCTG TAGGCTACAG GAGCTGTCTG AACAACTGGA GGGAAAGTTC 
TACAAGAATT CCTGTGAGAT GTTTTCTGCT GGAGAGAACA AAATACAGAC AACTGAGGAT 
GCCACCGATA GCTCCAGCAA AACGATGTCA ATTAAAGAAA GGTTGGCATT GCTGAAGAAA 
AGTGGTGAAG AAGATTGGAA GAGCAGGCTC AGTAAAAAGC AGGAATATGC AAAAGTGTCT 
GCCACTGATC GTAGCGCACA GATGCAAGAA GTTGAGCAGC TGTTGAAAAA GGAACCTGTA 
TATGCTTCTA CTTACTCTCC TGTTATACCT GCTGTCCATA AATTTCATCC TTTTCTACCT 
ATTAATCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148341.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004109.1 Gallus gallus supervillin (SVIL), transcript variant X9, mRNA.

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CloneID	OGa146731
Clone ID Related Accession (Same CDS sequence)	XM_025148341.1 , XM_025148349.1 , XM_025148342.1 , XM_025148343.1 , XM_025148344.1
Accession Version	XM_025148341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004109.1
CDS	551..7180
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148341.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148341.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148349.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004117.1 Gallus gallus supervillin (SVIL), transcript variant X17, mRNA.

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CloneID	OGa146731
Clone ID Related Accession (Same CDS sequence)	XM_025148341.1 , XM_025148349.1 , XM_025148342.1 , XM_025148343.1 , XM_025148344.1
Accession Version	XM_025148349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004117.1
CDS	228..6857
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148349.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148349.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148342.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004110.1 Gallus gallus supervillin (SVIL), transcript variant X10, mRNA.

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CloneID	OGa146731
Clone ID Related Accession (Same CDS sequence)	XM_025148341.1 , XM_025148349.1 , XM_025148342.1 , XM_025148343.1 , XM_025148344.1
Accession Version	XM_025148342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004110.1
CDS	415..7044
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148342.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148342.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148343.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004111.1 Gallus gallus supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OGa146731
Clone ID Related Accession (Same CDS sequence)	XM_025148341.1 , XM_025148349.1 , XM_025148342.1 , XM_025148343.1 , XM_025148344.1
Accession Version	XM_025148343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004111.1
CDS	459..7088
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148343.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148343.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148344.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004112.1 Gallus gallus supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OGa146731
Clone ID Related Accession (Same CDS sequence)	XM_025148341.1 , XM_025148349.1 , XM_025148342.1 , XM_025148343.1 , XM_025148344.1
Accession Version	XM_025148344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004112.1
CDS	209..6838
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148344.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148344.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148345.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004113.1 Gallus gallus supervillin (SVIL), transcript variant X13, mRNA.

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CloneID	OGa146732
Clone ID Related Accession (Same CDS sequence)	XM_025148345.1
Accession Version	XM_025148345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CCTTGCAGGC ATCAGCACAC 
CAAAAAATAC TAGCTAAAGA GGAGATAAGA GCAGCCAAAG AGGCGATGGG GCAAGATCAA 
TCCGATGAAC CAGATTCTTC CACATTAAGC TTGGCAGAAA AGATGGCTTT GTTTAACAAA 
CTGTCTCAGC CAGTATCCAG GGCTATATCC ACAAGGAGTC GGGGAGATAT TAGGCACAGA 
AGGATGAATA CTCGTTACCA GACCCAGCCA GTCACTCTTG GAGAAGTTGA GCAGATGCAA 
TGGAAAAAGA GTAAACCGGT GCAAAACGAA AGTGGAAAAC TAACTCCCCT GTCAAGTACA 
GTTTCAACAT CAGTTTCAGC AATGGCTTCT GCCCTTTCTG CACTGTTTGC TGGAGACATG 
CATGCGAAGT CACGTAGTGA TGGAAGTGTG AGCGCTGCCA CAAAAGATGA GTTGAGATTC 
CATGCTTCAG CAGAAAGCTC AGACTCATCA GGAAAACGCA CACAGAAGTC AGAACAGTGG 
CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA AGAGCATCAA AGAAGCATGA AGAAGAAGGA 
AAGAGGTTTC TAGCACATGA GGCTAATGAA ATCAGAAAGT ACAGCTCCTT TGAGGATGAA 
ACCACACATC CTGTTCTTGA AAAAACAGGA GACTACCATA AAGAGGCAGC CTACAGCTTC 
CTGAGGAAGG GCAGCATGGA ACTGTTTAGT TCACAACCAC TTTTTCAGCC TCTTGAAAGG 
AAGGAAATTG ATACCATCAT GCAAGACCAC GTGGAGCCTA CCTCTGAACT CACCAGCACA 
CCAGCAACGA GGACACTTTC CCAGACTACA GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG 
TCTGAACAAC TGGAGGGAAA GTTCTACAAG AATTCCTGTG AGATGTTTTC TGCTGGAGAG 
AACAAAATAC AGACAACTGA GGATGCCACC GATAGCTCCA GCAAAACGAT GTCAATTAAA 
GAAAGGTTGG CATTGCTGAA GAAAAGTGGT GAAGAAGATT GGAAGAGCAG GCTCAGTAAA 
AAGCAGGAAT ATGCAAAAGT GTCTGCCACT GATCGTAGCG CACAGATGCA AGAAGTTGAG 
CAGCTGTTGA AAAAGGAACC TGTATATGCT TCTACTTACT CTCCTGTTAT ACCTGCTGTC 
CATAAATTTC ATCCTTTTCT ACCTATTAAT CAGAGGATAG CAGATAATCA GGAGAGTCAA 
ATGACTATAG AAGAAAGAAA ACACCTCATC ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA 
GGTAAAGGCG CAGCAAATGA CTCCACGCAG TTCACCGTAG CTGGTCGAAT GGTAAAAAAA 
GGCCTGGCTT CTCCAAGTGC ACTAACACCA GTAGCATCCC CTTATGGTAA CAGGCAGAAG 
TCTACAACTC CGGTCACAAA GCCCTTGGAA GAAATTGAAG CTAGGCCTGA TATGCAATTG 
GAATCAGATA TGAAGCTTGA CAAGCTGGAG TCATTCTTGG GAAGGCTGAA TAACAAAGTC 
GCTGGAATGC AAGAGACAGT GTTGACAGTT ACAGGAAAAT CTGTGAAAGA GGTGATGAAG 
CTGGATGATG ATGAAACATT TTCCAAATTT TACCGCCGTG TGGATTTCCC TTCCTCCTCT 
GTGCCTTTGG ACCTTGATGA GGACTTTGAT GCCATCTTTG ACCCTTATGC ACCAAAGTTA 
ATATCCTCTG TAGCAGAGCA CAAACGTGCA GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC 
AGAAACCCCC TGAAAATGCT GGCAGCAAGA GAAGATATTC TTCATGAGTA CACTGAACAA 
AGACTGAATG TTGCCTTCAT GGAGTCAAAG CGAATGAAAG TAGAAAAAAT GTCTGCAAAC 
TCAAATTTCT CAGAAGTAGC ACTTGCTGGT TTGGCAAGCA AGGAGAACTT CAGCAACGTT 
AGTCTGCGGA GTGTTAACCT AACAGAGCAA AATTCTAACA ACAGTGCTGT GCCATACAAG 
AAGTTAATGC TGCTGCAAAT TAAAGGAAGA AGACATGTTC AAACAAGATT AGTTGAACCA 
AGGGCCTCAT CACTGAACAG TGGAGACTGT TTCCTATTGT TAACTCCACA TCTCTGTTTC 
TTATGGGTAG GAGAGTTTGC AAATGTCATA GAAAAAGCAA AGGCTTCAGA ACTTGCAACT 
TTGATTCAGA CAAAGAGGGA ACTTGGCTGT AGAGCTTCTT ATATACAGAC TATAGAAGAA 
GGAATAAACA CCCATACCCA TGCAGCCAAA GACTTCTGGA AACTCCTTGG TGGCCAAGCA 
AATTACCAGT CTGCTGGAAG ACCTGAAGAG GATGAAATGT ATGAAGCTGC CATAATAGAA 
ACTAATTGCA TTTACCGTCT TGTAGAGGAT AAACTCATTC CTGAAGATGA CTACTGGGGA 
AAGATGCCAA AATGTACCCT GCTACAACCA AAAGAGGTGC TCGTATTTGA TTTTGGCAGT 
GAAGTGTATG TCTGGCATGG AAAAGAAGTT ACTTTAGCAC AAAGAAAAGT GGCATTCCAG 
CTGGCAAAAC ACTTATGGAA TGGCACCTTC GACTACTCAA ATTGTGACAT AAATCCTCTG 
GACCCAGGAG AATGCAATCC CCTCATACCC AGAAAGGGAC AAGGACGTCC AGACTGGGCT 
GTGTTTGGCA GGCTCACAGA ACATAATGAG ACCATACTGT TCAAAGAAAA ATTCCTTGAT 
TGGACAGAGC TGAAGAAACT CAATGAGAAG AATTCTAGTG AATCCCTTCA CCAGAAGGAA 
GAATCCAAAT CTGATTCTAA ACCATATGAT GTCATGCTAA TGGTACCTGT GCCCCAAACT 
GCTGTAGGCA CGGTTCTGGA TGGAATGAAT ATTGGCCGGG GCTATGGACT TGTAGAAGGA 
GAAGATGGGA GGCAGTTTGA AATTATCACT GCATCTGTGG ATGTCTGGCA CATCCTGGAG 
TTTGATTATA GTAGATTGCC TAAACAAAGC ATAGGACAGT TCCATGAGGG AGACACATAC 
GTGGTGAAGT GGAAGTACAT GGTGAGCACT ACAGTTGGAA GCAGACAAAA AGGAGAGCAA 
CAAGTAAGAG CTGTTGGAAA AGAGAAATGT GTGTATTTCT TTTGGCAAGG AAGGCACTCC 
ACAGTGAGTG AGAAAGGGAC ATCGGCACTG ATGACAGTGG AACTTGATGA GGAGAGAGGA 
GCACAGGTAC AAGTGCTTCA AGGGAAAGAA CTACCATGCT TCCTGCAGTG CTTCCAAGGA 
GGGATGATTG TGCATGCTGG AAGAAGGGAA GAGGAAGAAG AAAATGCACA AAGTGACTGG 
AGACTATATT GCGTGCGAGG AGAAGTTCCC AATGAAGGAA ACTTACTTGA AGTAGCATGT 
CACTGCAGCA GCCTGCGTTC CCGGACATCC ATGATTGTCC TCAATATAAA TAAAGCTCTT 
ATCTACCTGT GGCATGGCTG CAAAGCACAA TCTCACACCA AGGATGTAGG AAGAACAGCA 
GCCAATAAAA TAAAAGAACA ATGTCCGCTG GAAGCAGGGC TGCACAGCAG CAGCAAGGTG 
ACAATACACG AATGTGATGA AGGGTCAGAG CCTTTGGGAT TCTGGGATGC ATTAGGAAGG 
CGAGATCGAA AGGCCTATGA TTGCATGTTG CAAGATCCGG GAAAGTTTAA TTTCACCCCC 
CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA GAATTCTCAG CCACTGAGTA CGTTTACCCT 
TCAAGAGACC CTACTGTCAT CAATTCTATG CCCTTCTTGC AAGAGGATCT TTACACTGCC 
CCACAGCCAG CACTGTTCCT TGTTGACAAT CACCATGAAG TGTACCTGTG GCAAGGCTGG 
TGGCCTGTGG AAAACAAAAT CACTGGATCA GCTCGAATCA GGTGGGCTAC AGACAGGAAA 
TGCGCCATGG AGACCGTGCT CCAGTACTGC AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG 
TCCTATCTCA TTCATGCCGG CCTAGAGCCT CTGACCTTCA CTAATATGTT CCCAAGTTGG 
GAACACAGGG AAGACATTGC AGAGATCACA GAAATGGATG CTGACGTTTC TAATCAGATA 
ATCCTTGTAG AAGATGTGCT GGCCAAGCTG TGTAAAACTG TGTATCCCCT AGCTGATCTC 
TTAGCCAGGC CTTTACCTGA GGGAGTTGAT CCTCTGAAGC TTGAAATTTA TCTTTCAGAT 
GAGGACTTTG AGGTTGCATT AGAGATGACA AGAGAAGAGT ACAATGCGCT GCCATCTTGG 
AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004113.1
CDS	18..6596
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148345.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148345.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CCTTGCAGGC ATCAGCACAC 
CAAAAAATAC TAGCTAAAGA GGAGATAAGA GCAGCCAAAG AGGCGATGGG GCAAGATCAA 
TCCGATGAAC CAGATTCTTC CACATTAAGC TTGGCAGAAA AGATGGCTTT GTTTAACAAA 
CTGTCTCAGC CAGTATCCAG GGCTATATCC ACAAGGAGTC GGGGAGATAT TAGGCACAGA 
AGGATGAATA CTCGTTACCA GACCCAGCCA GTCACTCTTG GAGAAGTTGA GCAGATGCAA 
TGGAAAAAGA GTAAACCGGT GCAAAACGAA AGTGGAAAAC TAACTCCCCT GTCAAGTACA 
GTTTCAACAT CAGTTTCAGC AATGGCTTCT GCCCTTTCTG CACTGTTTGC TGGAGACATG 
CATGCGAAGT CACGTAGTGA TGGAAGTGTG AGCGCTGCCA CAAAAGATGA GTTGAGATTC 
CATGCTTCAG CAGAAAGCTC AGACTCATCA GGAAAACGCA CACAGAAGTC AGAACAGTGG 
CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA AGAGCATCAA AGAAGCATGA AGAAGAAGGA 
AAGAGGTTTC TAGCACATGA GGCTAATGAA ATCAGAAAGT ACAGCTCCTT TGAGGATGAA 
ACCACACATC CTGTTCTTGA AAAAACAGGA GACTACCATA AAGAGGCAGC CTACAGCTTC 
CTGAGGAAGG GCAGCATGGA ACTGTTTAGT TCACAACCAC TTTTTCAGCC TCTTGAAAGG 
AAGGAAATTG ATACCATCAT GCAAGACCAC GTGGAGCCTA CCTCTGAACT CACCAGCACA 
CCAGCAACGA GGACACTTTC CCAGACTACA GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG 
TCTGAACAAC TGGAGGGAAA GTTCTACAAG AATTCCTGTG AGATGTTTTC TGCTGGAGAG 
AACAAAATAC AGACAACTGA GGATGCCACC GATAGCTCCA GCAAAACGAT GTCAATTAAA 
GAAAGGTTGG CATTGCTGAA GAAAAGTGGT GAAGAAGATT GGAAGAGCAG GCTCAGTAAA 
AAGCAGGAAT ATGCAAAAGT GTCTGCCACT GATCGTAGCG CACAGATGCA AGAAGTTGAG 
CAGCTGTTGA AAAAGGAACC TGTATATGCT TCTACTTACT CTCCTGTTAT ACCTGCTGTC 
CATAAATTTC ATCCTTTTCT ACCTATTAAT CAGAGGATAG CAGATAATCA GGAGAGTCAA 
ATGACTATAG AAGAAAGAAA ACACCTCATC ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA 
GGTAAAGGCG CAGCAAATGA CTCCACGCAG TTCACCGTAG CTGGTCGAAT GGTAAAAAAA 
GGCCTGGCTT CTCCAAGTGC ACTAACACCA GTAGCATCCC CTTATGGTAA CAGGCAGAAG 
TCTACAACTC CGGTCACAAA GCCCTTGGAA GAAATTGAAG CTAGGCCTGA TATGCAATTG 
GAATCAGATA TGAAGCTTGA CAAGCTGGAG TCATTCTTGG GAAGGCTGAA TAACAAAGTC 
GCTGGAATGC AAGAGACAGT GTTGACAGTT ACAGGAAAAT CTGTGAAAGA GGTGATGAAG 
CTGGATGATG ATGAAACATT TTCCAAATTT TACCGCCGTG TGGATTTCCC TTCCTCCTCT 
GTGCCTTTGG ACCTTGATGA GGACTTTGAT GCCATCTTTG ACCCTTATGC ACCAAAGTTA 
ATATCCTCTG TAGCAGAGCA CAAACGTGCA GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC 
AGAAACCCCC TGAAAATGCT GGCAGCAAGA GAAGATATTC TTCATGAGTA CACTGAACAA 
AGACTGAATG TTGCCTTCAT GGAGTCAAAG CGAATGAAAG TAGAAAAAAT GTCTGCAAAC 
TCAAATTTCT CAGAAGTAGC ACTTGCTGGT TTGGCAAGCA AGGAGAACTT CAGCAACGTT 
AGTCTGCGGA GTGTTAACCT AACAGAGCAA AATTCTAACA ACAGTGCTGT GCCATACAAG 
AAGTTAATGC TGCTGCAAAT TAAAGGAAGA AGACATGTTC AAACAAGATT AGTTGAACCA 
AGGGCCTCAT CACTGAACAG TGGAGACTGT TTCCTATTGT TAACTCCACA TCTCTGTTTC 
TTATGGGTAG GAGAGTTTGC AAATGTCATA GAAAAAGCAA AGGCTTCAGA ACTTGCAACT 
TTGATTCAGA CAAAGAGGGA ACTTGGCTGT AGAGCTTCTT ATATACAGAC TATAGAAGAA 
GGAATAAACA CCCATACCCA TGCAGCCAAA GACTTCTGGA AACTCCTTGG TGGCCAAGCA 
AATTACCAGT CTGCTGGAAG ACCTGAAGAG GATGAAATGT ATGAAGCTGC CATAATAGAA 
ACTAATTGCA TTTACCGTCT TGTAGAGGAT AAACTCATTC CTGAAGATGA CTACTGGGGA 
AAGATGCCAA AATGTACCCT GCTACAACCA AAAGAGGTGC TCGTATTTGA TTTTGGCAGT 
GAAGTGTATG TCTGGCATGG AAAAGAAGTT ACTTTAGCAC AAAGAAAAGT GGCATTCCAG 
CTGGCAAAAC ACTTATGGAA TGGCACCTTC GACTACTCAA ATTGTGACAT AAATCCTCTG 
GACCCAGGAG AATGCAATCC CCTCATACCC AGAAAGGGAC AAGGACGTCC AGACTGGGCT 
GTGTTTGGCA GGCTCACAGA ACATAATGAG ACCATACTGT TCAAAGAAAA ATTCCTTGAT 
TGGACAGAGC TGAAGAAACT CAATGAGAAG AATTCTAGTG AATCCCTTCA CCAGAAGGAA 
GAATCCAAAT CTGATTCTAA ACCATATGAT GTCATGCTAA TGGTACCTGT GCCCCAAACT 
GCTGTAGGCA CGGTTCTGGA TGGAATGAAT ATTGGCCGGG GCTATGGACT TGTAGAAGGA 
GAAGATGGGA GGCAGTTTGA AATTATCACT GCATCTGTGG ATGTCTGGCA CATCCTGGAG 
TTTGATTATA GTAGATTGCC TAAACAAAGC ATAGGACAGT TCCATGAGGG AGACACATAC 
GTGGTGAAGT GGAAGTACAT GGTGAGCACT ACAGTTGGAA GCAGACAAAA AGGAGAGCAA 
CAAGTAAGAG CTGTTGGAAA AGAGAAATGT GTGTATTTCT TTTGGCAAGG AAGGCACTCC 
ACAGTGAGTG AGAAAGGGAC ATCGGCACTG ATGACAGTGG AACTTGATGA GGAGAGAGGA 
GCACAGGTAC AAGTGCTTCA AGGGAAAGAA CTACCATGCT TCCTGCAGTG CTTCCAAGGA 
GGGATGATTG TGCATGCTGG AAGAAGGGAA GAGGAAGAAG AAAATGCACA AAGTGACTGG 
AGACTATATT GCGTGCGAGG AGAAGTTCCC AATGAAGGAA ACTTACTTGA AGTAGCATGT 
CACTGCAGCA GCCTGCGTTC CCGGACATCC ATGATTGTCC TCAATATAAA TAAAGCTCTT 
ATCTACCTGT GGCATGGCTG CAAAGCACAA TCTCACACCA AGGATGTAGG AAGAACAGCA 
GCCAATAAAA TAAAAGAACA ATGTCCGCTG GAAGCAGGGC TGCACAGCAG CAGCAAGGTG 
ACAATACACG AATGTGATGA AGGGTCAGAG CCTTTGGGAT TCTGGGATGC ATTAGGAAGG 
CGAGATCGAA AGGCCTATGA TTGCATGTTG CAAGATCCGG GAAAGTTTAA TTTCACCCCC 
CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA GAATTCTCAG CCACTGAGTA CGTTTACCCT 
TCAAGAGACC CTACTGTCAT CAATTCTATG CCCTTCTTGC AAGAGGATCT TTACACTGCC 
CCACAGCCAG CACTGTTCCT TGTTGACAAT CACCATGAAG TGTACCTGTG GCAAGGCTGG 
TGGCCTGTGG AAAACAAAAT CACTGGATCA GCTCGAATCA GGTGGGCTAC AGACAGGAAA 
TGCGCCATGG AGACCGTGCT CCAGTACTGC AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG 
TCCTATCTCA TTCATGCCGG CCTAGAGCCT CTGACCTTCA CTAATATGTT CCCAAGTTGG 
GAACACAGGG AAGACATTGC AGAGATCACA GAAATGGATG CTGACGTTTC TAATCAGATA 
ATCCTTGTAG AAGATGTGCT GGCCAAGCTG TGTAAAACTG TGTATCCCCT AGCTGATCTC 
TTAGCCAGGC CTTTACCTGA GGGAGTTGAT CCTCTGAAGC TTGAAATTTA TCTTTCAGAT 
GAGGACTTTG AGGTTGCATT AGAGATGACA AGAGAAGAGT ACAATGCGCT GCCATCTTGG 
AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148346.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004114.1 Gallus gallus supervillin (SVIL), transcript variant X14, mRNA.

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CloneID	OGa146733
Clone ID Related Accession (Same CDS sequence)	XM_025148346.1
Accession Version	XM_025148346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004114.1
CDS	553..7122
Translation	MLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148346.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148346.1

ORF Insert Sequence:

ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGAGGATA GCAGATAATC AGGAGAGTCA AATGACTATA 
GAAGAAAGAA AACACCTCAT CACAGCTCGA GAAGAAGCCT GGAAGTCCAG AGGTAAAGGC 
GCAGCAAATG ACTCCACGCA GTTCACCGTA GCTGGTCGAA TGGTAAAAAA AGGCCTGGCT 
TCTCCAAGTG CACTAACACC AGTAGCATCC CCTTATGGTA ACAGGCAGAA GTCTACAACT 
CCGGTCACAA AGCCCTTGGA AGAAATTGAA GCTAGGCCTG ATATGCAATT GGAATCAGAT 
ATGAAGCTTG ACAAGCTGGA GTCATTCTTG GGAAGGCTGA ATAACAAAGT CGCTGGAATG 
CAAGAGACAG TGTTGACAGT TACAGGAAAA TCTGTGAAAG AGGTGATGAA GCTGGATGAT 
GATGAAACAT TTTCCAAATT TTACCGCCGT GTGGATTTCC CTTCCTCCTC TGTGCCTTTG 
GACCTTGATG AGGACTTTGA TGCCATCTTT GACCCTTATG CACCAAAGTT AATATCCTCT 
GTAGCAGAGC ACAAACGTGC AGTGAGGCCT ATGCGCAGGG TTCAGTCCTC CAGAAACCCC 
CTGAAAATGC TGGCAGCAAG AGAAGATATT CTTCATGAGT ACACTGAACA AAGACTGAAT 
GTTGCCTTCA TGGAGTCAAA GCGAATGAAA GTAGAAAAAA TGTCTGCAAA CTCAAATTTC 
TCAGAAGTAG CACTTGCTGG TTTGGCAAGC AAGGAGAACT TCAGCAACGT TAGTCTGCGG 
AGTGTTAACC TAACAGAGCA AAATTCTAAC AACAGTGCTG TGCCATACAA GAAGTTAATG 
CTGCTGCAAA TTAAAGGAAG AAGACATGTT CAAACAAGAT TAGTTGAACC AAGGGCCTCA 
TCACTGAACA GTGGAGACTG TTTCCTATTG TTAACTCCAC ATCTCTGTTT CTTATGGGTA 
GGAGAGTTTG CAAATGTCAT AGAAAAAGCA AAGGCTTCAG AACTTGCAAC TTTGATTCAG 
ACAAAGAGGG AACTTGGCTG TAGAGCTTCT TATATACAGA CTATAGAAGA AGGAATAAAC 
ACCCATACCC ATGCAGCCAA AGACTTCTGG AAACTCCTTG GTGGCCAAGC AAATTACCAG 
TCTGCTGGAA GACCTGAAGA GGATGAAATG TATGAAGCTG CCATAATAGA AACTAATTGC 
ATTTACCGTC TTGTAGAGGA TAAACTCATT CCTGAAGATG ACTACTGGGG AAAGATGCCA 
AAATGTACCC TGCTACAACC AAAAGAGGTG CTCGTATTTG ATTTTGGCAG TGAAGTGTAT 
GTCTGGCATG GAAAAGAAGT TACTTTAGCA CAAAGAAAAG TGGCATTCCA GCTGGCAAAA 
CACTTATGGA ATGGCACCTT CGACTACTCA AATTGTGACA TAAATCCTCT GGACCCAGGA 
GAATGCAATC CCCTCATACC CAGAAAGGGA CAAGGACGTC CAGACTGGGC TGTGTTTGGC 
AGGCTCACAG AACATAATGA GACCATACTG TTCAAAGAAA AATTCCTTGA TTGGACAGAG 
CTGAAGAAAC TCAATGAGAA GAATTCTAGT GAATCCCTTC ACCAGAAGGA AGAATCCAAA 
TCTGATTCTA AACCATATGA TGTCATGCTA ATGGTACCTG TGCCCCAAAC TGCTGTAGGC 
ACGGTTCTGG ATGGAATGAA TATTGGCCGG GGCTATGGAC TTGTAGAAGG AGAAGATGGG 
AGGCAGTTTG AAATTATCAC TGCATCTGTG GATGTCTGGC ACATCCTGGA GTTTGATTAT 
AGTAGATTGC CTAAACAAAG CATAGGACAG TTCCATGAGG GAGACACATA CGTGGTGAAG 
TGGAAGTACA TGGTGAGCAC TACAGTTGGA AGCAGACAAA AAGGAGAGCA ACAAGTAAGA 
GCTGTTGGAA AAGAGAAATG TGTGTATTTC TTTTGGCAAG GAAGGCACTC CACAGTGAGT 
GAGAAAGGGA CATCGGCACT GATGACAGTG GAACTTGATG AGGAGAGAGG AGCACAGGTA 
CAAGTGCTTC AAGGGAAAGA ACTACCATGC TTCCTGCAGT GCTTCCAAGG AGGGATGATT 
GTGCATGCTG GAAGAAGGGA AGAGGAAGAA GAAAATGCAC AAAGTGACTG GAGACTATAT 
TGCGTGCGAG GAGAAGTTCC CAATGAAGGA AACTTACTTG AAGTAGCATG TCACTGCAGC 
AGCCTGCGTT CCCGGACATC CATGATTGTC CTCAATATAA ATAAAGCTCT TATCTACCTG 
TGGCATGGCT GCAAAGCACA ATCTCACACC AAGGATGTAG GAAGAACAGC AGCCAATAAA 
ATAAAAGAAC AATGTCCGCT GGAAGCAGGG CTGCACAGCA GCAGCAAGGT GACAATACAC 
GAATGTGATG AAGGGTCAGA GCCTTTGGGA TTCTGGGATG CATTAGGAAG GCGAGATCGA 
AAGGCCTATG ATTGCATGTT GCAAGATCCG GGAAAGTTTA ATTTCACCCC CCGCCTCTTC 
AGCCTCAGTA GTTCTTCGGG AGAATTCTCA GCCACTGAGT ACGTTTACCC TTCAAGAGAC 
CCTACTGTCA TCAATTCTAT GCCCTTCTTG CAAGAGGATC TTTACACTGC CCCACAGCCA 
GCACTGTTCC TTGTTGACAA TCACCATGAA GTGTACCTGT GGCAAGGCTG GTGGCCTGTG 
GAAAACAAAA TCACTGGATC AGCTCGAATC AGGTGGGCTA CAGACAGGAA ATGCGCCATG 
GAGACCGTGC TCCAGTACTG CAAAGGAAAA AATGTAAAGA AGCCCCCAAA GTCCTATCTC 
ATTCATGCCG GCCTAGAGCC TCTGACCTTC ACTAATATGT TCCCAAGTTG GGAACACAGG 
GAAGACATTG CAGAGATCAC AGAAATGGAT GCTGACGTTT CTAATCAGAT AATCCTTGTA 
GAAGATGTGC TGGCCAAGCT GTGTAAAACT GTGTATCCCC TAGCTGATCT CTTAGCCAGG 
CCTTTACCTG AGGGAGTTGA TCCTCTGAAG CTTGAAATTT ATCTTTCAGA TGAGGACTTT 
GAGGTTGCAT TAGAGATGAC AAGAGAAGAG TACAATGCGC TGCCATCTTG GAAGCAGGTT 
AATCTGAAAA AGGCAAAAGG ACTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148347.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004115.1 Gallus gallus supervillin (SVIL), transcript variant X15, mRNA.

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CloneID	OGa146734
Clone ID Related Accession (Same CDS sequence)	XM_025148347.1
Accession Version	XM_025148347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CCTTGCAGGC ATCAGCACAC 
CAAAAAATAC TAGCTAAAGA GGAGATAAGA GCAGCCAAAG AGGCGATGGG GCAAGATCAA 
TCCGATGAAC CAGATTCTTC CACATTAAGC TTGGCAGAAA AGATGGCTTT GTTTAACAAA 
CTGTCTCAGC CAGTATCCAG GGCTATATCC ACAAGGAGTC GGGGAGATAT TAGGCACAGA 
AGGATGAATA CTCGTTACCA GACCCAGCCA GTCACTCTTG GAGAAGTTGA GCAGGTGCAA 
AACGAAAGTG GAAAACTAAC TCCCCTGTCA AGTACAGTTT CAACATCAGT TTCAGCAATG 
GCTTCTGCCC TTTCTGCACT GTTTGCTGGA GACATGCATG CGAAGTCACG TAGTGATGGA 
AGTGTGAGCG CTGCCACAAA AGATGAGTTG AGATTCCATG CTTCAGCAGA AAGCTCAGAC 
TCATCAGGAA AACGCACACA GAAGTCAGAA CAGTGGCAGC CCTCAATGGA AGTGCTGGAA 
AGCAAAAGAG CATCAAAGAA GCATGAAGAA GAAGGAAAGA GGTTTCTAGC ACATGAGGCT 
AATGAAATCA GAAAGTACAG CTCCTTTGAG GATGAAACCA CACATCCTGT TCTTGAAAAA 
ACAGGAGACT ACCATAAAGA GGCAGCCTAC AGCTTCCTGA GGAAGGGCAG CATGGAACTG 
TTTAGTTCAC AACCACTTTT TCAGCCTCTT GAAAGGAAGG AAATTGATAC CATCATGCAA 
GACCACGTGG AGCCTACCTC TGAACTCACC AGCACACCAG CAACGAGGAC ACTTTCCCAG 
ACTACAGCTG CAGCATCCTG TAGGCTACAG GAGCTGTCTG AACAACTGGA GGGAAAGTTC 
TACAAGAATT CCTGTGAGAT GTTTTCTGCT GGAGAGAACA AAATACAGAC AACTGAGGAT 
GCCACCGATA GCTCCAGCAA AACGATGTCA ATTAAAGAAA GGTTGGCATT GCTGAAGAAA 
AGTGGTGAAG AAGATTGGAA GAGCAGGCTC AGTAAAAAGC AGGAATATGC AAAAGTGTCT 
GCCACTGATC GTAGCGCACA GATGCAAGAA GTTGAGCAGC TGTTGAAAAA GGAACCTGTA 
TATGCTTCTA CTTACTCTCC TGTTATACCT GCTGTCCATA AATTTCATCC TTTTCTACCT 
ATTAATCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004115.1
CDS	18..6572
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148347.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148347.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CCTTGCAGGC ATCAGCACAC 
CAAAAAATAC TAGCTAAAGA GGAGATAAGA GCAGCCAAAG AGGCGATGGG GCAAGATCAA 
TCCGATGAAC CAGATTCTTC CACATTAAGC TTGGCAGAAA AGATGGCTTT GTTTAACAAA 
CTGTCTCAGC CAGTATCCAG GGCTATATCC ACAAGGAGTC GGGGAGATAT TAGGCACAGA 
AGGATGAATA CTCGTTACCA GACCCAGCCA GTCACTCTTG GAGAAGTTGA GCAGGTGCAA 
AACGAAAGTG GAAAACTAAC TCCCCTGTCA AGTACAGTTT CAACATCAGT TTCAGCAATG 
GCTTCTGCCC TTTCTGCACT GTTTGCTGGA GACATGCATG CGAAGTCACG TAGTGATGGA 
AGTGTGAGCG CTGCCACAAA AGATGAGTTG AGATTCCATG CTTCAGCAGA AAGCTCAGAC 
TCATCAGGAA AACGCACACA GAAGTCAGAA CAGTGGCAGC CCTCAATGGA AGTGCTGGAA 
AGCAAAAGAG CATCAAAGAA GCATGAAGAA GAAGGAAAGA GGTTTCTAGC ACATGAGGCT 
AATGAAATCA GAAAGTACAG CTCCTTTGAG GATGAAACCA CACATCCTGT TCTTGAAAAA 
ACAGGAGACT ACCATAAAGA GGCAGCCTAC AGCTTCCTGA GGAAGGGCAG CATGGAACTG 
TTTAGTTCAC AACCACTTTT TCAGCCTCTT GAAAGGAAGG AAATTGATAC CATCATGCAA 
GACCACGTGG AGCCTACCTC TGAACTCACC AGCACACCAG CAACGAGGAC ACTTTCCCAG 
ACTACAGCTG CAGCATCCTG TAGGCTACAG GAGCTGTCTG AACAACTGGA GGGAAAGTTC 
TACAAGAATT CCTGTGAGAT GTTTTCTGCT GGAGAGAACA AAATACAGAC AACTGAGGAT 
GCCACCGATA GCTCCAGCAA AACGATGTCA ATTAAAGAAA GGTTGGCATT GCTGAAGAAA 
AGTGGTGAAG AAGATTGGAA GAGCAGGCTC AGTAAAAAGC AGGAATATGC AAAAGTGTCT 
GCCACTGATC GTAGCGCACA GATGCAAGAA GTTGAGCAGC TGTTGAAAAA GGAACCTGTA 
TATGCTTCTA CTTACTCTCC TGTTATACCT GCTGTCCATA AATTTCATCC TTTTCTACCT 
ATTAATCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148348.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004116.1 Gallus gallus supervillin (SVIL), transcript variant X16, mRNA.

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CloneID	OGa146735
Clone ID Related Accession (Same CDS sequence)	XM_025148348.1
Accession Version	XM_025148348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTATT 
ATGTGTGCTG AAATACCAAC TGCTGACGAT GAAGAAAAAT TAGATGACCG AGCCAAACTA 
AGTGTAGCTG CAAAGAGACT GCTTTTTAGA GAAATGGAAA AATCTTTTGA AATGAAGAAT 
ATTCCTAAAC CACCTACGAG AAGCTCAGCA GTAGAGAGAA GAATGCGGCG TTTGCAGGAC 
AGATCACACA CGCAGCCTAT TACCAGTGAG GAAGTGGTCA TTGCAGCTAC TGAAACTACC 
CCTGCTTCAC GTTCTGTGAA TACCCACACA GTAGTGGCAA GAGTACCTAG TCCCACTCTA 
GTTAAGAGCA CTGTACAACC TACCAGCTTG CAGGCATCAG CACACCAAAA AATACTAGCT 
AAAGAGGAGA TAAGAGCAGC CAAAGAGGCG ATGGGGCAAG ATCAATCCGA TGAACCAGAT 
TCTTCCACAT TAAGCTTGGC AGAAAAGATG GCTTTGTTTA ACAAACTGTC TCAGCCAGTA 
TCCAGGGCTA TATCCACAAG GAGTCGGGGA GATATTAGGC ACAGAAGGAT GAATACTCGT 
TACCAGACCC AGCCAGTCAC TCTTGGAGAA GTTGAGCAGA TGCAATGGAA AAAGAGTAAA 
CCGGTGCAAA ACGAAAGTGG AAAACTAACT CCCCTGTCAA GTACAGTTTC AACATCAGTT 
TCAGCAATGG CTTCTGCCCT TTCTGCACTG TTTGCTGGAG ACATGCATGC GAAGTCACGT 
AGTGATGGAA GTGTGAGCGC TGCCACAAAA GATGAGTTGA GATTCCATGC TTCAGCAGAA 
AGCTCAGACT CATCAGGAAA ACGCACACAG AAGTCAGAAC AGTGGCAGCC CTCAATGGAA 
GTGCTGGAAA GCAAAAGAGC ATCAAAGAAG CATGAAGAAG AAGGAAAGAG GTTTCTAGCA 
CATGAGGCTA ATGAAATCAG AAAGTACAGC TCCTTTGAGG ATGAAACCAC ACATCCTGTT 
CTTGAAAAAA CAGGAGACTA CCATAAAGAG GCAGCCTACA GCTTCCTGAG GAAGGGCAGC 
ATGGAACTGT TTAGTTCACA ACCACTTTTT CAGCCTCTTG AAAGGAAGGA AATTGATACC 
ATCATGCAAG ACCACGTGGA GCCTACCTCT GAACTCACCA GCACACCAGC AACGAGGACA 
CTTTCCCAGA CTACAGCTGC AGCATCCTGT AGGCTACAGG AGCTGTCTGA ACAACTGGAG 
GGAAAGTTCT ACAAGAATTC CTGTGAGATG TTTTCTGCTG GAGAGAACAA AATACAGACA 
ACTGAGGATG CCACCGATAG CTCCAGCAAA ACGATGTCAA TTAAAGAAAG GTTGGCATTG 
CTGAAGAAAA GTGGTGAAGA AGATTGGAAG AGCAGGCTCA GTAAAAAGCA GGAATATGCA 
AAAGTGTCTG CCACTGATCG TAGCGCACAG ATGCAAGAAG TTGAGCAGCT GTTGAAAAAG 
GAACCTGTAT ATGCTTCTAC TTACTCTCCT GTTATACCTG CTGTCCATAA ATTTCATCCT 
TTTCTACCTA TTAATCAGAG GATAGCAGAT AATCAGGAGA GTCAAATGAC TATAGAAGAA 
AGAAAACACC TCATCACAGC TCGAGAAGAA GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA 
AATGACTCCA CGCAGTTCAC CGTAGCTGGT CGAATGGTAA AAAAAGGCCT GGCTTCTCCA 
AGTGCACTAA CACCAGTAGC ATCCCCTTAT GGTAACAGGC AGAAGTCTAC AACTCCGGTC 
ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG CCTGATATGC AATTGGAATC AGATATGAAG 
CTTGACAAGC TGGAGTCATT CTTGGGAAGG CTGAATAACA AAGTCGCTGG AATGCAAGAG 
ACAGTGTTGA CAGTTACAGG AAAATCTGTG AAAGAGGTGA TGAAGCTGGA TGATGATGAA 
ACATTTTCCA AATTTTACCG CCGTGTGGAT TTCCCTTCCT CCTCTGTGCC TTTGGACCTT 
GATGAGGACT TTGATGCCAT CTTTGACCCT TATGCACCAA AGTTAATATC CTCTGTAGCA 
GAGCACAAAC GTGCAGTGAG GCCTATGCGC AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA 
ATGCTGGCAG CAAGAGAAGA TATTCTTCAT GAGTACACTG AACAAAGACT GAATGTTGCC 
TTCATGGAGT CAAAGCGAAT GAAAGTAGAA AAAATGTCTG CAAACTCAAA TTTCTCAGAA 
GTAGCACTTG CTGGTTTGGC AAGCAAGGAG AACTTCAGCA ACGTTAGTCT GCGGAGTGTT 
AACCTAACAG AGCAAAATTC TAACAACAGT GCTGTGCCAT ACAAGAAGTT AATGCTGCTG 
CAAATTAAAG GAAGAAGACA TGTTCAAACA AGATTAGTTG AACCAAGGGC CTCATCACTG 
AACAGTGGAG ACTGTTTCCT ATTGTTAACT CCACATCTCT GTTTCTTATG GGTAGGAGAG 
TTTGCAAATG TCATAGAAAA AGCAAAGGCT TCAGAACTTG CAACTTTGAT TCAGACAAAG 
AGGGAACTTG GCTGTAGAGC TTCTTATATA CAGACTATAG AAGAAGGAAT AAACACCCAT 
ACCCATGCAG CCAAAGACTT CTGGAAACTC CTTGGTGGCC AAGCAAATTA CCAGTCTGCT 
GGAAGACCTG AAGAGGATGA AATGTATGAA GCTGCCATAA TAGAAACTAA TTGCATTTAC 
CGTCTTGTAG AGGATAAACT CATTCCTGAA GATGACTACT GGGGAAAGAT GCCAAAATGT 
ACCCTGCTAC AACCAAAAGA GGTGCTCGTA TTTGATTTTG GCAGTGAAGT GTATGTCTGG 
CATGGAAAAG AAGTTACTTT AGCACAAAGA AAAGTGGCAT TCCAGCTGGC AAAACACTTA 
TGGAATGGCA CCTTCGACTA CTCAAATTGT GACATAAATC CTCTGGACCC AGGAGAATGC 
AATCCCCTCA TACCCAGAAA GGGACAAGGA CGTCCAGACT GGGCTGTGTT TGGCAGGCTC 
ACAGAACATA ATGAGACCAT ACTGTTCAAA GAAAAATTCC TTGATTGGAC AGAGCTGAAG 
AAACTCAATG AGAAGAATTC TAGTGAATCC CTTCACCAGA AGGAAGAATC CAAATCTGAT 
TCTAAACCAT ATGATGTCAT GCTAATGGTA CCTGTGCCCC AAACTGCTGT AGGCACGGTT 
CTGGATGGAA TGAATATTGG CCGGGGCTAT GGACTTGTAG AAGGAGAAGA TGGGAGGCAG 
TTTGAAATTA TCACTGCATC TGTGGATGTC TGGCACATCC TGGAGTTTGA TTATAGTAGA 
TTGCCTAAAC AAAGCATAGG ACAGTTCCAT GAGGGAGACA CATACGTGGT GAAGTGGAAG 
TACATGGTGA GCACTACAGT TGGAAGCAGA CAAAAAGGAG AGCAACAAGT AAGAGCTGTT 
GGAAAAGAGA AATGTGTGTA TTTCTTTTGG CAAGGAAGGC ACTCCACAGT GAGTGAGAAA 
GGGACATCGG CACTGATGAC AGTGGAACTT GATGAGGAGA GAGGAGCACA GGTACAAGTG 
CTTCAAGGGA AAGAACTACC ATGCTTCCTG CAGTGCTTCC AAGGAGGGAT GATTGTGCAT 
GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT GCACAAAGTG ACTGGAGACT ATATTGCGTG 
CGAGGAGAAG TTCCCAATGA AGGAAACTTA CTTGAAGTAG CATGTCACTG CAGCAGCCTG 
CGTTCCCGGA CATCCATGAT TGTCCTCAAT ATAAATAAAG CTCTTATCTA CCTGTGGCAT 
GGCTGCAAAG CACAATCTCA CACCAAGGAT GTAGGAAGAA CAGCAGCCAA TAAAATAAAA 
GAACAATGTC CGCTGGAAGC AGGGCTGCAC AGCAGCAGCA AGGTGACAAT ACACGAATGT 
GATGAAGGGT CAGAGCCTTT GGGATTCTGG GATGCATTAG GAAGGCGAGA TCGAAAGGCC 
TATGATTGCA TGTTGCAAGA TCCGGGAAAG TTTAATTTCA CCCCCCGCCT CTTCAGCCTC 
AGTAGTTCTT CGGGAGAATT CTCAGCCACT GAGTACGTTT ACCCTTCAAG AGACCCTACT 
GTCATCAATT CTATGCCCTT CTTGCAAGAG GATCTTTACA CTGCCCCACA GCCAGCACTG 
TTCCTTGTTG ACAATCACCA TGAAGTGTAC CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC 
AAAATCACTG GATCAGCTCG AATCAGGTGG GCTACAGACA GGAAATGCGC CATGGAGACC 
GTGCTCCAGT ACTGCAAAGG AAAAAATGTA AAGAAGCCCC CAAAGTCCTA TCTCATTCAT 
GCCGGCCTAG AGCCTCTGAC CTTCACTAAT ATGTTCCCAA GTTGGGAACA CAGGGAAGAC 
ATTGCAGAGA TCACAGAAAT GGATGCTGAC GTTTCTAATC AGATAATCCT TGTAGAAGAT 
GTGCTGGCCA AGCTGTGTAA AACTGTGTAT CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA 
CCTGAGGGAG TTGATCCTCT GAAGCTTGAA ATTTATCTTT CAGATGAGGA CTTTGAGGTT 
GCATTAGAGA TGACAAGAGA AGAGTACAAT GCGCTGCCAT CTTGGAAGCA GGTTAATCTG 
AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004116.1
CDS	19..6522
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148348.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148348.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTATT 
ATGTGTGCTG AAATACCAAC TGCTGACGAT GAAGAAAAAT TAGATGACCG AGCCAAACTA 
AGTGTAGCTG CAAAGAGACT GCTTTTTAGA GAAATGGAAA AATCTTTTGA AATGAAGAAT 
ATTCCTAAAC CACCTACGAG AAGCTCAGCA GTAGAGAGAA GAATGCGGCG TTTGCAGGAC 
AGATCACACA CGCAGCCTAT TACCAGTGAG GAAGTGGTCA TTGCAGCTAC TGAAACTACC 
CCTGCTTCAC GTTCTGTGAA TACCCACACA GTAGTGGCAA GAGTACCTAG TCCCACTCTA 
GTTAAGAGCA CTGTACAACC TACCAGCTTG CAGGCATCAG CACACCAAAA AATACTAGCT 
AAAGAGGAGA TAAGAGCAGC CAAAGAGGCG ATGGGGCAAG ATCAATCCGA TGAACCAGAT 
TCTTCCACAT TAAGCTTGGC AGAAAAGATG GCTTTGTTTA ACAAACTGTC TCAGCCAGTA 
TCCAGGGCTA TATCCACAAG GAGTCGGGGA GATATTAGGC ACAGAAGGAT GAATACTCGT 
TACCAGACCC AGCCAGTCAC TCTTGGAGAA GTTGAGCAGA TGCAATGGAA AAAGAGTAAA 
CCGGTGCAAA ACGAAAGTGG AAAACTAACT CCCCTGTCAA GTACAGTTTC AACATCAGTT 
TCAGCAATGG CTTCTGCCCT TTCTGCACTG TTTGCTGGAG ACATGCATGC GAAGTCACGT 
AGTGATGGAA GTGTGAGCGC TGCCACAAAA GATGAGTTGA GATTCCATGC TTCAGCAGAA 
AGCTCAGACT CATCAGGAAA ACGCACACAG AAGTCAGAAC AGTGGCAGCC CTCAATGGAA 
GTGCTGGAAA GCAAAAGAGC ATCAAAGAAG CATGAAGAAG AAGGAAAGAG GTTTCTAGCA 
CATGAGGCTA ATGAAATCAG AAAGTACAGC TCCTTTGAGG ATGAAACCAC ACATCCTGTT 
CTTGAAAAAA CAGGAGACTA CCATAAAGAG GCAGCCTACA GCTTCCTGAG GAAGGGCAGC 
ATGGAACTGT TTAGTTCACA ACCACTTTTT CAGCCTCTTG AAAGGAAGGA AATTGATACC 
ATCATGCAAG ACCACGTGGA GCCTACCTCT GAACTCACCA GCACACCAGC AACGAGGACA 
CTTTCCCAGA CTACAGCTGC AGCATCCTGT AGGCTACAGG AGCTGTCTGA ACAACTGGAG 
GGAAAGTTCT ACAAGAATTC CTGTGAGATG TTTTCTGCTG GAGAGAACAA AATACAGACA 
ACTGAGGATG CCACCGATAG CTCCAGCAAA ACGATGTCAA TTAAAGAAAG GTTGGCATTG 
CTGAAGAAAA GTGGTGAAGA AGATTGGAAG AGCAGGCTCA GTAAAAAGCA GGAATATGCA 
AAAGTGTCTG CCACTGATCG TAGCGCACAG ATGCAAGAAG TTGAGCAGCT GTTGAAAAAG 
GAACCTGTAT ATGCTTCTAC TTACTCTCCT GTTATACCTG CTGTCCATAA ATTTCATCCT 
TTTCTACCTA TTAATCAGAG GATAGCAGAT AATCAGGAGA GTCAAATGAC TATAGAAGAA 
AGAAAACACC TCATCACAGC TCGAGAAGAA GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA 
AATGACTCCA CGCAGTTCAC CGTAGCTGGT CGAATGGTAA AAAAAGGCCT GGCTTCTCCA 
AGTGCACTAA CACCAGTAGC ATCCCCTTAT GGTAACAGGC AGAAGTCTAC AACTCCGGTC 
ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG CCTGATATGC AATTGGAATC AGATATGAAG 
CTTGACAAGC TGGAGTCATT CTTGGGAAGG CTGAATAACA AAGTCGCTGG AATGCAAGAG 
ACAGTGTTGA CAGTTACAGG AAAATCTGTG AAAGAGGTGA TGAAGCTGGA TGATGATGAA 
ACATTTTCCA AATTTTACCG CCGTGTGGAT TTCCCTTCCT CCTCTGTGCC TTTGGACCTT 
GATGAGGACT TTGATGCCAT CTTTGACCCT TATGCACCAA AGTTAATATC CTCTGTAGCA 
GAGCACAAAC GTGCAGTGAG GCCTATGCGC AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA 
ATGCTGGCAG CAAGAGAAGA TATTCTTCAT GAGTACACTG AACAAAGACT GAATGTTGCC 
TTCATGGAGT CAAAGCGAAT GAAAGTAGAA AAAATGTCTG CAAACTCAAA TTTCTCAGAA 
GTAGCACTTG CTGGTTTGGC AAGCAAGGAG AACTTCAGCA ACGTTAGTCT GCGGAGTGTT 
AACCTAACAG AGCAAAATTC TAACAACAGT GCTGTGCCAT ACAAGAAGTT AATGCTGCTG 
CAAATTAAAG GAAGAAGACA TGTTCAAACA AGATTAGTTG AACCAAGGGC CTCATCACTG 
AACAGTGGAG ACTGTTTCCT ATTGTTAACT CCACATCTCT GTTTCTTATG GGTAGGAGAG 
TTTGCAAATG TCATAGAAAA AGCAAAGGCT TCAGAACTTG CAACTTTGAT TCAGACAAAG 
AGGGAACTTG GCTGTAGAGC TTCTTATATA CAGACTATAG AAGAAGGAAT AAACACCCAT 
ACCCATGCAG CCAAAGACTT CTGGAAACTC CTTGGTGGCC AAGCAAATTA CCAGTCTGCT 
GGAAGACCTG AAGAGGATGA AATGTATGAA GCTGCCATAA TAGAAACTAA TTGCATTTAC 
CGTCTTGTAG AGGATAAACT CATTCCTGAA GATGACTACT GGGGAAAGAT GCCAAAATGT 
ACCCTGCTAC AACCAAAAGA GGTGCTCGTA TTTGATTTTG GCAGTGAAGT GTATGTCTGG 
CATGGAAAAG AAGTTACTTT AGCACAAAGA AAAGTGGCAT TCCAGCTGGC AAAACACTTA 
TGGAATGGCA CCTTCGACTA CTCAAATTGT GACATAAATC CTCTGGACCC AGGAGAATGC 
AATCCCCTCA TACCCAGAAA GGGACAAGGA CGTCCAGACT GGGCTGTGTT TGGCAGGCTC 
ACAGAACATA ATGAGACCAT ACTGTTCAAA GAAAAATTCC TTGATTGGAC AGAGCTGAAG 
AAACTCAATG AGAAGAATTC TAGTGAATCC CTTCACCAGA AGGAAGAATC CAAATCTGAT 
TCTAAACCAT ATGATGTCAT GCTAATGGTA CCTGTGCCCC AAACTGCTGT AGGCACGGTT 
CTGGATGGAA TGAATATTGG CCGGGGCTAT GGACTTGTAG AAGGAGAAGA TGGGAGGCAG 
TTTGAAATTA TCACTGCATC TGTGGATGTC TGGCACATCC TGGAGTTTGA TTATAGTAGA 
TTGCCTAAAC AAAGCATAGG ACAGTTCCAT GAGGGAGACA CATACGTGGT GAAGTGGAAG 
TACATGGTGA GCACTACAGT TGGAAGCAGA CAAAAAGGAG AGCAACAAGT AAGAGCTGTT 
GGAAAAGAGA AATGTGTGTA TTTCTTTTGG CAAGGAAGGC ACTCCACAGT GAGTGAGAAA 
GGGACATCGG CACTGATGAC AGTGGAACTT GATGAGGAGA GAGGAGCACA GGTACAAGTG 
CTTCAAGGGA AAGAACTACC ATGCTTCCTG CAGTGCTTCC AAGGAGGGAT GATTGTGCAT 
GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT GCACAAAGTG ACTGGAGACT ATATTGCGTG 
CGAGGAGAAG TTCCCAATGA AGGAAACTTA CTTGAAGTAG CATGTCACTG CAGCAGCCTG 
CGTTCCCGGA CATCCATGAT TGTCCTCAAT ATAAATAAAG CTCTTATCTA CCTGTGGCAT 
GGCTGCAAAG CACAATCTCA CACCAAGGAT GTAGGAAGAA CAGCAGCCAA TAAAATAAAA 
GAACAATGTC CGCTGGAAGC AGGGCTGCAC AGCAGCAGCA AGGTGACAAT ACACGAATGT 
GATGAAGGGT CAGAGCCTTT GGGATTCTGG GATGCATTAG GAAGGCGAGA TCGAAAGGCC 
TATGATTGCA TGTTGCAAGA TCCGGGAAAG TTTAATTTCA CCCCCCGCCT CTTCAGCCTC 
AGTAGTTCTT CGGGAGAATT CTCAGCCACT GAGTACGTTT ACCCTTCAAG AGACCCTACT 
GTCATCAATT CTATGCCCTT CTTGCAAGAG GATCTTTACA CTGCCCCACA GCCAGCACTG 
TTCCTTGTTG ACAATCACCA TGAAGTGTAC CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC 
AAAATCACTG GATCAGCTCG AATCAGGTGG GCTACAGACA GGAAATGCGC CATGGAGACC 
GTGCTCCAGT ACTGCAAAGG AAAAAATGTA AAGAAGCCCC CAAAGTCCTA TCTCATTCAT 
GCCGGCCTAG AGCCTCTGAC CTTCACTAAT ATGTTCCCAA GTTGGGAACA CAGGGAAGAC 
ATTGCAGAGA TCACAGAAAT GGATGCTGAC GTTTCTAATC AGATAATCCT TGTAGAAGAT 
GTGCTGGCCA AGCTGTGTAA AACTGTGTAT CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA 
CCTGAGGGAG TTGATCCTCT GAAGCTTGAA ATTTATCTTT CAGATGAGGA CTTTGAGGTT 
GCATTAGAGA TGACAAGAGA AGAGTACAAT GCGCTGCCAT CTTGGAAGCA GGTTAATCTG 
AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025148356.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004124.1 Gallus gallus supervillin (SVIL), transcript variant X24, mRNA.

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CloneID	OGa146742
Clone ID Related Accession (Same CDS sequence)	XM_025148356.1
Accession Version	XM_025148356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGCAGAG GATAGCAGAT 
AATCAGGAGA GTCAAATGAC TATAGAAGAA AGAAAACACC TCATCACAGC TCGAGAAGAA 
GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA AATGACTCCA CGCAGTTCAC CGTAGCTGGT 
CGAATGGTAA AAAAAGGCCT GGCTTCTCCA AGTGCACTAA CACCAGTAGC ATCCCCTTAT 
GGTAACAGGC AGAAGTCTAC AACTCCGGTC ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG 
CCTGATATGC AATTGGAATC AGATATGAAG CTTGACAAGC TGGAGTCATT CTTGGGAAGG 
CTGAATAACA AAGTCGCTGG AATGCAAGAG ACAGTGTTGA CAGTTACAGG AAAATCTGTG 
AAAGAGGTGA TGAAGCTGGA TGATGATGAA ACATTTTCCA AATTTTACCG CCGTGTGGAT 
TTCCCTTCCT CCTCTGTGCC TTTGGACCTT GATGAGGACT TTGATGCCAT CTTTGACCCT 
TATGCACCAA AGTTAATATC CTCTGTAGCA GAGCACAAAC GTGCAGTGAG GCCTATGCGC 
AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA ATGCTGGCAG CAAGAGAAGA TATTCTTCAT 
GAGTACACTG AACAAAGACT GAATGTTGCC TTCATGGAGT CAAAGCGAAT GAAAGTAGAA 
AAAATGTCTG CAAACTCAAA TTTCTCAGAA GTAGCACTTG CTGGTTTGGC AAGCAAGGAG 
AACTTCAGCA ACGTTAGTCT GCGGAGTGTT AACCTAACAG AGCAAAATTC TAACAACAGT 
GCTGTGCCAT ACAAGAAGTT AATGCTGCTG CAAATTAAAG GAAGAAGACA TGTTCAAACA 
AGATTAGTTG AACCAAGGGC CTCATCACTG AACAGTGGAG ACTGTTTCCT ATTGTTAACT 
CCACATCTCT GTTTCTTATG GGTAGGAGAG TTTGCAAATG TCATAGAAAA AGCAAAGGCT 
TCAGAACTTG CAACTTTGAT TCAGACAAAG AGGGAACTTG GCTGTAGAGC TTCTTATATA 
CAGACTATAG AAGAAGGAAT AAACACCCAT ACCCATGCAG CCAAAGACTT CTGGAAACTC 
CTTGGTGGCC AAGCAAATTA CCAGTCTGCT GGAAGACCTG AAGAGGATGA AATGTATGAA 
GCTGCCATAA TAGAAACTAA TTGCATTTAC CGTCTTGTAG AGGATAAACT CATTCCTGAA 
GATGACTACT GGGGAAAGAT GCCAAAATGT ACCCTGCTAC AACCAAAAGA GGTGCTCGTA 
TTTGATTTTG GCAGTGAAGT GTATGTCTGG CATGGAAAAG AAGTTACTTT AGCACAAAGA 
AAAGTGGCAT TCCAGCTGGC AAAACACTTA TGGAATGGCA CCTTCGACTA CTCAAATTGT 
GACATAAATC CTCTGGACCC AGGAGAATGC AATCCCCTCA TACCCAGAAA GGGACAAGGA 
CGTCCAGACT GGGCTGTGTT TGGCAGGCTC ACAGAACATA ATGAGACCAT ACTGTTCAAA 
GAAAAATTCC TTGATTGGAC AGAGCTGAAG AAACTCAATG AGAAGAATTC TAGTGAATCC 
CTTCACCAGA AGGAAGAATC CAAATCTGAT TCTAAACCAT ATGATGTCAT GCTAATGGTA 
CCTGTGCCCC AAACTGCTGT AGGCACGGTT CTGGATGGAA TGAATATTGG CCGGGGCTAT 
GGACTTGTAG AAGGAGAAGA TGGGAGGCAG TTTGAAATTA TCACTGCATC TGTGGATGTC 
TGGCACATCC TGGAGTTTGA TTATAGTAGA TTGCCTAAAC AAAGCATAGG ACAGTTCCAT 
GAGGGAGACA CATACGTGGT GAAGTGGAAG TACATGGTGA GCACTACAGT TGGAAGCAGA 
CAAAAAGGAG AGCAACAAGT AAGAGCTGTT GGAAAAGAGA AATGTGTGTA TTTCTTTTGG 
CAAGGAAGGC ACTCCACAGT GAGTGAGAAA GGGACATCGG CACTGATGAC AGTGGAACTT 
GATGAGGAGA GAGGAGCACA GGTACAAGTG CTTCAAGGGA AAGAACTACC ATGCTTCCTG 
CAGTGCTTCC AAGGAGGGAT GATTGTGCAT GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT 
GCACAAAGTG ACTGGAGACT ATATTGCGTG CGAGGAGAAG TTCCCAATGA AGGAAACTTA 
CTTGAAGTAG CATGTCACTG CAGCAGCCTG CGTTCCCGGA CATCCATGAT TGTCCTCAAT 
ATAAATAAAG CTCTTATCTA CCTGTGGCAT GGCTGCAAAG CACAATCTCA CACCAAGGAT 
GTAGGAAGAA CAGCAGCCAA TAAAATAAAA GAACAATGTC CGCTGGAAGC AGGGCTGCAC 
AGCAGCAGCA AGGTGACAAT ACACGAATGT GATGAAGGGT CAGAGCCTTT GGGATTCTGG 
GATGCATTAG GAAGGCGAGA TCGAAAGGCC TATGATTGCA TGTTGCAAGA TCCGGGAAAG 
TTTAATTTCA CCCCCCGCCT CTTCAGCCTC AGTAGTTCTT CGGGAGAATT CTCAGCCACT 
GAGTACGTTT ACCCTTCAAG AGACCCTACT GTCATCAATT CTATGCCCTT CTTGCAAGAG 
GATCTTTACA CTGCCCCACA GCCAGCACTG TTCCTTGTTG ACAATCACCA TGAAGTGTAC 
CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC AAAATCACTG GATCAGCTCG AATCAGGTGG 
GCTACAGACA GGAAATGCGC CATGGAGACC GTGCTCCAGT ACTGCAAAGG AAAAAATGTA 
AAGAAGCCCC CAAAGTCCTA TCTCATTCAT GCCGGCCTAG AGCCTCTGAC CTTCACTAAT 
ATGTTCCCAA GTTGGGAACA CAGGGAAGAC ATTGCAGAGA TCACAGAAAT GGATGCTGAC 
GTTTCTAATC AGATAATCCT TGTAGAAGAT GTGCTGGCCA AGCTGTGTAA AACTGTGTAT 
CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA CCTGAGGGAG TTGATCCTCT GAAGCTTGAA 
ATTTATCTTT CAGATGAGGA CTTTGAGGTT GCATTAGAGA TGACAAGAGA AGAGTACAAT 
GCGCTGCCAT CTTGGAAGCA GGTTAATCTG AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004124.1
CDS	19..6852
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025148356.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148356.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGCAGAG GATAGCAGAT 
AATCAGGAGA GTCAAATGAC TATAGAAGAA AGAAAACACC TCATCACAGC TCGAGAAGAA 
GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA AATGACTCCA CGCAGTTCAC CGTAGCTGGT 
CGAATGGTAA AAAAAGGCCT GGCTTCTCCA AGTGCACTAA CACCAGTAGC ATCCCCTTAT 
GGTAACAGGC AGAAGTCTAC AACTCCGGTC ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG 
CCTGATATGC AATTGGAATC AGATATGAAG CTTGACAAGC TGGAGTCATT CTTGGGAAGG 
CTGAATAACA AAGTCGCTGG AATGCAAGAG ACAGTGTTGA CAGTTACAGG AAAATCTGTG 
AAAGAGGTGA TGAAGCTGGA TGATGATGAA ACATTTTCCA AATTTTACCG CCGTGTGGAT 
TTCCCTTCCT CCTCTGTGCC TTTGGACCTT GATGAGGACT TTGATGCCAT CTTTGACCCT 
TATGCACCAA AGTTAATATC CTCTGTAGCA GAGCACAAAC GTGCAGTGAG GCCTATGCGC 
AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA ATGCTGGCAG CAAGAGAAGA TATTCTTCAT 
GAGTACACTG AACAAAGACT GAATGTTGCC TTCATGGAGT CAAAGCGAAT GAAAGTAGAA 
AAAATGTCTG CAAACTCAAA TTTCTCAGAA GTAGCACTTG CTGGTTTGGC AAGCAAGGAG 
AACTTCAGCA ACGTTAGTCT GCGGAGTGTT AACCTAACAG AGCAAAATTC TAACAACAGT 
GCTGTGCCAT ACAAGAAGTT AATGCTGCTG CAAATTAAAG GAAGAAGACA TGTTCAAACA 
AGATTAGTTG AACCAAGGGC CTCATCACTG AACAGTGGAG ACTGTTTCCT ATTGTTAACT 
CCACATCTCT GTTTCTTATG GGTAGGAGAG TTTGCAAATG TCATAGAAAA AGCAAAGGCT 
TCAGAACTTG CAACTTTGAT TCAGACAAAG AGGGAACTTG GCTGTAGAGC TTCTTATATA 
CAGACTATAG AAGAAGGAAT AAACACCCAT ACCCATGCAG CCAAAGACTT CTGGAAACTC 
CTTGGTGGCC AAGCAAATTA CCAGTCTGCT GGAAGACCTG AAGAGGATGA AATGTATGAA 
GCTGCCATAA TAGAAACTAA TTGCATTTAC CGTCTTGTAG AGGATAAACT CATTCCTGAA 
GATGACTACT GGGGAAAGAT GCCAAAATGT ACCCTGCTAC AACCAAAAGA GGTGCTCGTA 
TTTGATTTTG GCAGTGAAGT GTATGTCTGG CATGGAAAAG AAGTTACTTT AGCACAAAGA 
AAAGTGGCAT TCCAGCTGGC AAAACACTTA TGGAATGGCA CCTTCGACTA CTCAAATTGT 
GACATAAATC CTCTGGACCC AGGAGAATGC AATCCCCTCA TACCCAGAAA GGGACAAGGA 
CGTCCAGACT GGGCTGTGTT TGGCAGGCTC ACAGAACATA ATGAGACCAT ACTGTTCAAA 
GAAAAATTCC TTGATTGGAC AGAGCTGAAG AAACTCAATG AGAAGAATTC TAGTGAATCC 
CTTCACCAGA AGGAAGAATC CAAATCTGAT TCTAAACCAT ATGATGTCAT GCTAATGGTA 
CCTGTGCCCC AAACTGCTGT AGGCACGGTT CTGGATGGAA TGAATATTGG CCGGGGCTAT 
GGACTTGTAG AAGGAGAAGA TGGGAGGCAG TTTGAAATTA TCACTGCATC TGTGGATGTC 
TGGCACATCC TGGAGTTTGA TTATAGTAGA TTGCCTAAAC AAAGCATAGG ACAGTTCCAT 
GAGGGAGACA CATACGTGGT GAAGTGGAAG TACATGGTGA GCACTACAGT TGGAAGCAGA 
CAAAAAGGAG AGCAACAAGT AAGAGCTGTT GGAAAAGAGA AATGTGTGTA TTTCTTTTGG 
CAAGGAAGGC ACTCCACAGT GAGTGAGAAA GGGACATCGG CACTGATGAC AGTGGAACTT 
GATGAGGAGA GAGGAGCACA GGTACAAGTG CTTCAAGGGA AAGAACTACC ATGCTTCCTG 
CAGTGCTTCC AAGGAGGGAT GATTGTGCAT GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT 
GCACAAAGTG ACTGGAGACT ATATTGCGTG CGAGGAGAAG TTCCCAATGA AGGAAACTTA 
CTTGAAGTAG CATGTCACTG CAGCAGCCTG CGTTCCCGGA CATCCATGAT TGTCCTCAAT 
ATAAATAAAG CTCTTATCTA CCTGTGGCAT GGCTGCAAAG CACAATCTCA CACCAAGGAT 
GTAGGAAGAA CAGCAGCCAA TAAAATAAAA GAACAATGTC CGCTGGAAGC AGGGCTGCAC 
AGCAGCAGCA AGGTGACAAT ACACGAATGT GATGAAGGGT CAGAGCCTTT GGGATTCTGG 
GATGCATTAG GAAGGCGAGA TCGAAAGGCC TATGATTGCA TGTTGCAAGA TCCGGGAAAG 
TTTAATTTCA CCCCCCGCCT CTTCAGCCTC AGTAGTTCTT CGGGAGAATT CTCAGCCACT 
GAGTACGTTT ACCCTTCAAG AGACCCTACT GTCATCAATT CTATGCCCTT CTTGCAAGAG 
GATCTTTACA CTGCCCCACA GCCAGCACTG TTCCTTGTTG ACAATCACCA TGAAGTGTAC 
CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC AAAATCACTG GATCAGCTCG AATCAGGTGG 
GCTACAGACA GGAAATGCGC CATGGAGACC GTGCTCCAGT ACTGCAAAGG AAAAAATGTA 
AAGAAGCCCC CAAAGTCCTA TCTCATTCAT GCCGGCCTAG AGCCTCTGAC CTTCACTAAT 
ATGTTCCCAA GTTGGGAACA CAGGGAAGAC ATTGCAGAGA TCACAGAAAT GGATGCTGAC 
GTTTCTAATC AGATAATCCT TGTAGAAGAT GTGCTGGCCA AGCTGTGTAA AACTGTGTAT 
CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA CCTGAGGGAG TTGATCCTCT GAAGCTTGAA 
ATTTATCTTT CAGATGAGGA CTTTGAGGTT GCATTAGAGA TGACAAGAGA AGAGTACAAT 
GCGCTGCCAT CTTGGAAGCA GGTTAATCTG AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282091.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137577.1 Gallus gallus supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OGa31968
Clone ID Related Accession (Same CDS sequence)	XM_015282091.2 , XM_015282091.1
Accession Version	XM_015282091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137577.1
CDS	20..6931
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282091.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282091.2

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282091.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137577.1 Gallus gallus supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OGa31968
Clone ID Related Accession (Same CDS sequence)	XM_015282091.2 , XM_015282091.1
Accession Version	XM_015282091.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137577.1
CDS	18..6929
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282091.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282091.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282092.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137578.1 Gallus gallus supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OGa31969
Clone ID Related Accession (Same CDS sequence)	XM_015282092.1
Accession Version	XM_015282092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137578.1
CDS	18..6929
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282092.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282092.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGATGCAA TGGAAAAAGA GTAAACCGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGGAACC TGTATATGCT 
TCTACTTACT CTCCTGTTAT ACCTGCTGTC CATAAATTTC ATCCTTTTCT ACCTATTAAT 
CAGCAGAGGA TAGCAGATAA TCAGGAGAGT CAAATGACTA TAGAAGAAAG AAAACACCTC 
ATCACAGCTC GAGAAGAAGC CTGGAAGTCC AGAGGTAAAG GCGCAGCAAA TGACTCCACG 
CAGTTCACCG TAGCTGGTCG AATGGTAAAA AAAGGCCTGG CTTCTCCAAG TGCACTAACA 
CCAGTAGCAT CCCCTTATGG TAACAGGCAG AAGTCTACAA CTCCGGTCAC AAAGCCCTTG 
GAAGAAATTG AAGCTAGGCC TGATATGCAA TTGGAATCAG ATATGAAGCT TGACAAGCTG 
GAGTCATTCT TGGGAAGGCT GAATAACAAA GTCGCTGGAA TGCAAGAGAC AGTGTTGACA 
GTTACAGGAA AATCTGTGAA AGAGGTGATG AAGCTGGATG ATGATGAAAC ATTTTCCAAA 
TTTTACCGCC GTGTGGATTT CCCTTCCTCC TCTGTGCCTT TGGACCTTGA TGAGGACTTT 
GATGCCATCT TTGACCCTTA TGCACCAAAG TTAATATCCT CTGTAGCAGA GCACAAACGT 
GCAGTGAGGC CTATGCGCAG GGTTCAGTCC TCCAGAAACC CCCTGAAAAT GCTGGCAGCA 
AGAGAAGATA TTCTTCATGA GTACACTGAA CAAAGACTGA ATGTTGCCTT CATGGAGTCA 
AAGCGAATGA AAGTAGAAAA AATGTCTGCA AACTCAAATT TCTCAGAAGT AGCACTTGCT 
GGTTTGGCAA GCAAGGAGAA CTTCAGCAAC GTTAGTCTGC GGAGTGTTAA CCTAACAGAG 
CAAAATTCTA ACAACAGTGC TGTGCCATAC AAGAAGTTAA TGCTGCTGCA AATTAAAGGA 
AGAAGACATG TTCAAACAAG ATTAGTTGAA CCAAGGGCCT CATCACTGAA CAGTGGAGAC 
TGTTTCCTAT TGTTAACTCC ACATCTCTGT TTCTTATGGG TAGGAGAGTT TGCAAATGTC 
ATAGAAAAAG CAAAGGCTTC AGAACTTGCA ACTTTGATTC AGACAAAGAG GGAACTTGGC 
TGTAGAGCTT CTTATATACA GACTATAGAA GAAGGAATAA ACACCCATAC CCATGCAGCC 
AAAGACTTCT GGAAACTCCT TGGTGGCCAA GCAAATTACC AGTCTGCTGG AAGACCTGAA 
GAGGATGAAA TGTATGAAGC TGCCATAATA GAAACTAATT GCATTTACCG TCTTGTAGAG 
GATAAACTCA TTCCTGAAGA TGACTACTGG GGAAAGATGC CAAAATGTAC CCTGCTACAA 
CCAAAAGAGG TGCTCGTATT TGATTTTGGC AGTGAAGTGT ATGTCTGGCA TGGAAAAGAA 
GTTACTTTAG CACAAAGAAA AGTGGCATTC CAGCTGGCAA AACACTTATG GAATGGCACC 
TTCGACTACT CAAATTGTGA CATAAATCCT CTGGACCCAG GAGAATGCAA TCCCCTCATA 
CCCAGAAAGG GACAAGGACG TCCAGACTGG GCTGTGTTTG GCAGGCTCAC AGAACATAAT 
GAGACCATAC TGTTCAAAGA AAAATTCCTT GATTGGACAG AGCTGAAGAA ACTCAATGAG 
AAGAATTCTA GTGAATCCCT TCACCAGAAG GAAGAATCCA AATCTGATTC TAAACCATAT 
GATGTCATGC TAATGGTACC TGTGCCCCAA ACTGCTGTAG GCACGGTTCT GGATGGAATG 
AATATTGGCC GGGGCTATGG ACTTGTAGAA GGAGAAGATG GGAGGCAGTT TGAAATTATC 
ACTGCATCTG TGGATGTCTG GCACATCCTG GAGTTTGATT ATAGTAGATT GCCTAAACAA 
AGCATAGGAC AGTTCCATGA GGGAGACACA TACGTGGTGA AGTGGAAGTA CATGGTGAGC 
ACTACAGTTG GAAGCAGACA AAAAGGAGAG CAACAAGTAA GAGCTGTTGG AAAAGAGAAA 
TGTGTGTATT TCTTTTGGCA AGGAAGGCAC TCCACAGTGA GTGAGAAAGG GACATCGGCA 
CTGATGACAG TGGAACTTGA TGAGGAGAGA GGAGCACAGG TACAAGTGCT TCAAGGGAAA 
GAACTACCAT GCTTCCTGCA GTGCTTCCAA GGAGGGATGA TTGTGCATGC TGGAAGAAGG 
GAAGAGGAAG AAGAAAATGC ACAAAGTGAC TGGAGACTAT ATTGCGTGCG AGGAGAAGTT 
CCCAATGAAG GAAACTTACT TGAAGTAGCA TGTCACTGCA GCAGCCTGCG TTCCCGGACA 
TCCATGATTG TCCTCAATAT AAATAAAGCT CTTATCTACC TGTGGCATGG CTGCAAAGCA 
CAATCTCACA CCAAGGATGT AGGAAGAACA GCAGCCAATA AAATAAAAGA ACAATGTCCG 
CTGGAAGCAG GGCTGCACAG CAGCAGCAAG GTGACAATAC ACGAATGTGA TGAAGGGTCA 
GAGCCTTTGG GATTCTGGGA TGCATTAGGA AGGCGAGATC GAAAGGCCTA TGATTGCATG 
TTGCAAGATC CGGGAAAGTT TAATTTCACC CCCCGCCTCT TCAGCCTCAG TAGTTCTTCG 
GGAGAATTCT CAGCCACTGA GTACGTTTAC CCTTCAAGAG ACCCTACTGT CATCAATTCT 
ATGCCCTTCT TGCAAGAGGA TCTTTACACT GCCCCACAGC CAGCACTGTT CCTTGTTGAC 
AATCACCATG AAGTGTACCT GTGGCAAGGC TGGTGGCCTG TGGAAAACAA AATCACTGGA 
TCAGCTCGAA TCAGGTGGGC TACAGACAGG AAATGCGCCA TGGAGACCGT GCTCCAGTAC 
TGCAAAGGAA AAAATGTAAA GAAGCCCCCA AAGTCCTATC TCATTCATGC CGGCCTAGAG 
CCTCTGACCT TCACTAATAT GTTCCCAAGT TGGGAACACA GGGAAGACAT TGCAGAGATC 
ACAGAAATGG ATGCTGACGT TTCTAATCAG ATAATCCTTG TAGAAGATGT GCTGGCCAAG 
CTGTGTAAAA CTGTGTATCC CCTAGCTGAT CTCTTAGCCA GGCCTTTACC TGAGGGAGTT 
GATCCTCTGA AGCTTGAAAT TTATCTTTCA GATGAGGACT TTGAGGTTGC ATTAGAGATG 
ACAAGAGAAG AGTACAATGC GCTGCCATCT TGGAAGCAGG TTAATCTGAA AAAGGCAAAA 
GGACTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282093.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137579.1 Gallus gallus supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OGa31970
Clone ID Related Accession (Same CDS sequence)	XM_015282093.1
Accession Version	XM_015282093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGAA ATCCTTGGAT CTCCAGCTAT TTGGGAAATG TTAAACACTC TGAGGAGCAA 
GATTTTAACA AAGGAAACTC GGTTAGATTT AAACCTTCAC GACTGCTCCA CAGGAAGAGA 
GCGTTAAGTT TTCAGAAGGA GTTCTCATTT GAAGATAAAG AAGAACTTGC AAACAGCACA 
AAGGATATTG ATGCAAACCT CTTAGCAGTA CTTCCAAAAG TTTCAGAATT AAGAAAACTG 
TTTGAACCAA ACAACCAAGA GTTTTTGGAA ATGAAAAGAA AGGAGAGAAT AGCTAGACGC 
TTAGAGGGAA TTGAAAATGA TACGCAACCT ATGCTTCTAC AGAACTGCCC AGGGTCAGTC 
ACACACCGTT TACTAGAAGA AGACACACCA AGGTATATGC GTGCAACTGA TCCATACAGT 
CATCACTTAG GAAGATCAAA TGAAGAGGAG GAAGCTTCAG ATTCTTCTGT AGAGAAACAA 
CCCAGATCAT CCAGATACCG CACAGAAACC ACAGGAGGAA ATACAGAGTC TTTGTACAGT 
ACAGGAAACA TGGATACCCA TGAACTAGAG TCTAAAGCAG AAAGAATAGC TAGGTACAAG 
GCAGAGAGAC GGCGCCAACT GGCAGAAAAG TATGGATTAT CTCTTGATTC TGATTTGGAT 
TCTGACTACT CATCCAGATA TTCCCGGGCA AGAAAAGATC CTGACAGTGT GGAAAGAAAG 
GCATTGAGAA GTGAAAGGCA TGATGATGAA AACAAGGACT CTGGCTCCTT GTATTTGTCC 
AGGACTGAGG TGAAGGAGTC AAAGAGTGTG CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT 
GAAAAAGATG GTATTCCAGC TAAAGAAGAG CTTTCAAATG AAAAGAGTGA TAAAAGAGCA 
GATGATGACA GTGCCATTAG ACAGGCTTCT GACCCTTCAG CAACATTGGA TAGTTCTGTC 
TCACTAACAC TTTCTGGACG AGAGTCTTCC TCCTGTAATG AAGTGCCAAT ATCACCAAAG 
CAAAGCCCCC GAGAGTCTCT TTCTTCACCA AAGCGGGCAG CCTCACCAAT CCATTTGCAG 
AATGATCAGC CCTTGCACAG TAACATCAGA CAAAGTAGTG CAGGGAAAGC AAAACCTGAA 
TGGTTCCTTC AGAAAGATTC AGAAGGGGAC ACACCATCTC TTATCAGCTG GCCCTCCAGA 
GTAAAAGTTA GAGAGAAACT GGTGAAAGAG GAAAGTGCAC GTGGAAGCCC TGAACCTGTC 
ACAGACAGTG TATCTCAGAG AAAATCCCAG CCAGTGTCAG CCCACTACAT GCCTCTTCAG 
ACAGAAACGT CTGCCTTTGA TAGGGTTCTC AATCAGCCCG TCAGTGCAGT CCGCCAACCA 
ATACATGGGT ATATTCAGCC TGCTGGTGTT GCACACACGG CTCAGCTGAT GGCAACAGAA 
GAACCAGAAA GCATCTTCGT TGGCAGCCGC CTCCTTCCAG ACAGTGTCAG CCCCTTAACG 
AAATCAGCAG CAGCCACTGC ACCAGAGGGT GAAAAGAAAG AGACGCTGGC TTATGGAGCA 
AAGCCTGGCG AAGAAGAATC CCCAGAGGGT GAGAAGGCTT CCAAAGGCAG AAGACCAATT 
TTGCCATCTG AGATTCGCAA GCAAGGGAAG AGCCACGAAG GCCTCTTTGG AGGAGAAGAA 
CCAGATACCA GTGTGGGGAG CTCCTCATCT GCAAGCAAGC CGAAGGTTGG CTCAGATGTT 
CAGCAGGAGC AGGAATGCAA AAAGAAGCAG GCGCCTGAGC AGCAGCTGAA GACACCTGAG 
AACATGGAGA GGAGCGAAGC TGTCATGTAC ATACAGAGCG GGTCTGTATC AAAGCCAGCC 
CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA AAGCATAAAG TCCTGACTCG CTCCATGTCT 
GACTATACTG TGCCTCCTAA GCTAGAAGCT TTTCATAGTA AGAGCATGGA AGCAAACGCA 
GCAAATGGTG AGATTGGCAT GGTTGACACA AAGGTCTCTG TTGCCCAGCT CCGTAATGTC 
TTTCTGGAAA CTGCTCATGC CAACAAGAAA ACGGACCTCG AATCTCGGTT TGAGATGTCA 
ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT GACCGTGAAA GAGGGCCGCG AAGGCCGAGG 
CGCTATTTCA CTCCTGGTGA AAATAGAAAG ACTTCTGAGC GATTTAAAAC CCAACCTATA 
ACGTCAGCAG AACGAAAGGA GACGGATAGG TCTATTATGT GTGCTGAAAT ACCAACTGCT 
GACGATGAAG AAAAATTAGA TGACCGAGCC AAACTAAGTG TAGCTGCAAA GAGACTGCTT 
TTTAGAGAAA TGGAAAAATC TTTTGAAATG AAGAATATTC CTAAACCACC TACGAGAAGC 
TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG CAGGACAGAT CACACACGCA GCCTATTACC 
AGTGAGGAAG TGGTCATTGC AGCTACTGAA ACTACCCCTG CTTCACGTTC TGTGAATACC 
CACACAGTAG TGGCAAGAGT ACCTAGTCCC ACTCTAGTTA AGAGCACTGT ACAACCTACC 
AGCTTGCAGG CATCAGCACA CCAAAAAATA CTAGCTAAAG AGGAGATAAG AGCAGCCAAA 
GAGGCGATGG GGCAAGATCA ATCCGATGAA CCAGATTCTT CCACATTAAG CTTGGCAGAA 
AAGATGGCTT TGTTTAACAA ACTGTCTCAG CCAGTATCCA GGGCTATATC CACAAGGAGT 
CGGGGAGATA TTAGGCACAG AAGGATGAAT ACTCGTTACC AGACCCAGCC AGTCACTCTT 
GGAGAAGTTG AGCAGATGCA ATGGAAAAAG AGTAAACCGG TGCAAAACGA AAGTGGAAAA 
CTAACTCCCC TGTCAAGTAC AGTTTCAACA TCAGTTTCAG CAATGGCTTC TGCCCTTTCT 
GCACTGTTTG CTGGAGACAT GCATGCGAAG TCACGTAGTG ATGGAAGTGT GAGCGCTGCC 
ACAAAAGATG AGTTGAGATT CCATGCTTCA GCAGAAAGCT CAGACTCATC AGGAAAACGC 
ACACAGAAGT CAGAACAGTG GCAGCCCTCA ATGGAAGTGC TGGAAAGCAA AAGAGCATCA 
AAGAAGCATG AAGAAGAAGG AAAGAGGTTT CTAGCACATG AGGCTAATGA AATCAGAAAG 
TACAGCTCCT TTGAGGATGA AACCACACAT CCTGTTCTTG AAAAAACAGG AGACTACCAT 
AAAGAGGCAG CCTACAGCTT CCTGAGGAAG GGCAGCATGG AACTGTTTAG TTCACAACCA 
CTTTTTCAGC CTCTTGAAAG GAAGGAAATT GATACCATCA TGCAAGACCA CGTGGAGCCT 
ACCTCTGAAC TCACCAGCAC ACCAGCAACG AGGACACTTT CCCAGACTAC AGCTGCAGCA 
TCCTGTAGGC TACAGGAGCT GTCTGAACAA CTGGAGGGAA AGTTCTACAA GAATTCCTGT 
GAGATGTTTT CTGCTGGAGA GAACAAAATA CAGACAACTG AGGATGCCAC CGATAGCTCC 
AGCAAAACGA TGTCAATTAA AGAAAGGTTG GCATTGCTGA AGAAAAGTGG TGAAGAAGAT 
TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA TATGCAAAAG TGTCTGCCAC TGATCGTAGC 
GCACAGATGC AAGAAGTTGA GCAGCTGTTG AAAAAGGAAC CTGTATATGC TTCTACTTAC 
TCTCCTGTTA TACCTGCTGT CCATAAATTT CATCCTTTTC TACCTATTAA TCAGCAGAGG 
ATAGCAGATA ATCAGGAGAG TCAAATGACT ATAGAAGAAA GAAAACACCT CATCACAGCT 
CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA GGCGCAGCAA ATGACTCCAC GCAGTTCACC 
GTAGCTGGTC GAATGGTAAA AAAAGGCCTG GCTTCTCCAA GTGCACTAAC ACCAGTAGCA 
TCCCCTTATG GTAACAGGCA GAAGTCTACA ACTCCGGTCA CAAAGCCCTT GGAAGAAATT 
GAAGCTAGGC CTGATATGCA ATTGGAATCA GATATGAAGC TTGACAAGCT GGAGTCATTC 
TTGGGAAGGC TGAATAACAA AGTCGCTGGA ATGCAAGAGA CAGTGTTGAC AGTTACAGGA 
AAATCTGTGA AAGAGGTGAT GAAGCTGGAT GATGATGAAA CATTTTCCAA ATTTTACCGC 
CGTGTGGATT TCCCTTCCTC CTCTGTGCCT TTGGACCTTG ATGAGGACTT TGATGCCATC 
TTTGACCCTT ATGCACCAAA GTTAATATCC TCTGTAGCAG AGCACAAACG TGCAGTGAGG 
CCTATGCGCA GGGTTCAGTC CTCCAGAAAC CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT 
ATTCTTCATG AGTACACTGA ACAAAGACTG AATGTTGCCT TCATGGAGTC AAAGCGAATG 
AAAGTAGAAA AAATGTCTGC AAACTCAAAT TTCTCAGAAG TAGCACTTGC TGGTTTGGCA 
AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG CGGAGTGTTA ACCTAACAGA GCAAAATTCT 
AACAACAGTG CTGTGCCATA CAAGAAGTTA ATGCTGCTGC AAATTAAAGG AAGAAGACAT 
GTTCAAACAA GATTAGTTGA ACCAAGGGCC TCATCACTGA ACAGTGGAGA CTGTTTCCTA 
TTGTTAACTC CACATCTCTG TTTCTTATGG GTAGGAGAGT TTGCAAATGT CATAGAAAAA 
GCAAAGGCTT CAGAACTTGC AACTTTGATT CAGACAAAGA GGGAACTTGG CTGTAGAGCT 
TCTTATATAC AGACTATAGA AGAAGGAATA AACACCCATA CCCATGCAGC CAAAGACTTC 
TGGAAACTCC TTGGTGGCCA AGCAAATTAC CAGTCTGCTG GAAGACCTGA AGAGGATGAA 
ATGTATGAAG CTGCCATAAT AGAAACTAAT TGCATTTACC GTCTTGTAGA GGATAAACTC 
ATTCCTGAAG ATGACTACTG GGGAAAGATG CCAAAATGTA CCCTGCTACA ACCAAAAGAG 
GTGCTCGTAT TTGATTTTGG CAGTGAAGTG TATGTCTGGC ATGGAAAAGA AGTTACTTTA 
GCACAAAGAA AAGTGGCATT CCAGCTGGCA AAACACTTAT GGAATGGCAC CTTCGACTAC 
TCAAATTGTG ACATAAATCC TCTGGACCCA GGAGAATGCA ATCCCCTCAT ACCCAGAAAG 
GGACAAGGAC GTCCAGACTG GGCTGTGTTT GGCAGGCTCA CAGAACATAA TGAGACCATA 
CTGTTCAAAG AAAAATTCCT TGATTGGACA GAGCTGAAGA AACTCAATGA GAAGAATTCT 
AGTGAATCCC TTCACCAGAA GGAAGAATCC AAATCTGATT CTAAACCATA TGATGTCATG 
CTAATGGTAC CTGTGCCCCA AACTGCTGTA GGCACGGTTC TGGATGGAAT GAATATTGGC 
CGGGGCTATG GACTTGTAGA AGGAGAAGAT GGGAGGCAGT TTGAAATTAT CACTGCATCT 
GTGGATGTCT GGCACATCCT GGAGTTTGAT TATAGTAGAT TGCCTAAACA AAGCATAGGA 
CAGTTCCATG AGGGAGACAC ATACGTGGTG AAGTGGAAGT ACATGGTGAG CACTACAGTT 
GGAAGCAGAC AAAAAGGAGA GCAACAAGTA AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT 
TTCTTTTGGC AAGGAAGGCA CTCCACAGTG AGTGAGAAAG GGACATCGGC ACTGATGACA 
GTGGAACTTG ATGAGGAGAG AGGAGCACAG GTACAAGTGC TTCAAGGGAA AGAACTACCA 
TGCTTCCTGC AGTGCTTCCA AGGAGGGATG ATTGTGCATG CTGGAAGAAG GGAAGAGGAA 
GAAGAAAATG CACAAAGTGA CTGGAGACTA TATTGCGTGC GAGGAGAAGT TCCCAATGAA 
GGAAACTTAC TTGAAGTAGC ATGTCACTGC AGCAGCCTGC GTTCCCGGAC ATCCATGATT 
GTCCTCAATA TAAATAAAGC TCTTATCTAC CTGTGGCATG GCTGCAAAGC ACAATCTCAC 
ACCAAGGATG TAGGAAGAAC AGCAGCCAAT AAAATAAAAG AACAATGTCC GCTGGAAGCA 
GGGCTGCACA GCAGCAGCAA GGTGACAATA CACGAATGTG ATGAAGGGTC AGAGCCTTTG 
GGATTCTGGG ATGCATTAGG AAGGCGAGAT CGAAAGGCCT ATGATTGCAT GTTGCAAGAT 
CCGGGAAAGT TTAATTTCAC CCCCCGCCTC TTCAGCCTCA GTAGTTCTTC GGGAGAATTC 
TCAGCCACTG AGTACGTTTA CCCTTCAAGA GACCCTACTG TCATCAATTC TATGCCCTTC 
TTGCAAGAGG ATCTTTACAC TGCCCCACAG CCAGCACTGT TCCTTGTTGA CAATCACCAT 
GAAGTGTACC TGTGGCAAGG CTGGTGGCCT GTGGAAAACA AAATCACTGG ATCAGCTCGA 
ATCAGGTGGG CTACAGACAG GAAATGCGCC ATGGAGACCG TGCTCCAGTA CTGCAAAGGA 
AAAAATGTAA AGAAGCCCCC AAAGTCCTAT CTCATTCATG CCGGCCTAGA GCCTCTGACC 
TTCACTAATA TGTTCCCAAG TTGGGAACAC AGGGAAGACA TTGCAGAGAT CACAGAAATG 
GATGCTGACG TTTCTAATCA GATAATCCTT GTAGAAGATG TGCTGGCCAA GCTGTGTAAA 
ACTGTGTATC CCCTAGCTGA TCTCTTAGCC AGGCCTTTAC CTGAGGGAGT TGATCCTCTG 
AAGCTTGAAA TTTATCTTTC AGATGAGGAC TTTGAGGTTG CATTAGAGAT GACAAGAGAA 
GAGTACAATG CGCTGCCATC TTGGAAGCAG GTTAATCTGA AAAAGGCAAA AGGACTTTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137579.1
CDS	16..6918
Translation	MFRNPWISSYLGNVKHSEEQDFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282093.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282093.1

ORF Insert Sequence:

ATGTTCCGAA ATCCTTGGAT CTCCAGCTAT TTGGGAAATG TTAAACACTC TGAGGAGCAA 
GATTTTAACA AAGGAAACTC GGTTAGATTT AAACCTTCAC GACTGCTCCA CAGGAAGAGA 
GCGTTAAGTT TTCAGAAGGA GTTCTCATTT GAAGATAAAG AAGAACTTGC AAACAGCACA 
AAGGATATTG ATGCAAACCT CTTAGCAGTA CTTCCAAAAG TTTCAGAATT AAGAAAACTG 
TTTGAACCAA ACAACCAAGA GTTTTTGGAA ATGAAAAGAA AGGAGAGAAT AGCTAGACGC 
TTAGAGGGAA TTGAAAATGA TACGCAACCT ATGCTTCTAC AGAACTGCCC AGGGTCAGTC 
ACACACCGTT TACTAGAAGA AGACACACCA AGGTATATGC GTGCAACTGA TCCATACAGT 
CATCACTTAG GAAGATCAAA TGAAGAGGAG GAAGCTTCAG ATTCTTCTGT AGAGAAACAA 
CCCAGATCAT CCAGATACCG CACAGAAACC ACAGGAGGAA ATACAGAGTC TTTGTACAGT 
ACAGGAAACA TGGATACCCA TGAACTAGAG TCTAAAGCAG AAAGAATAGC TAGGTACAAG 
GCAGAGAGAC GGCGCCAACT GGCAGAAAAG TATGGATTAT CTCTTGATTC TGATTTGGAT 
TCTGACTACT CATCCAGATA TTCCCGGGCA AGAAAAGATC CTGACAGTGT GGAAAGAAAG 
GCATTGAGAA GTGAAAGGCA TGATGATGAA AACAAGGACT CTGGCTCCTT GTATTTGTCC 
AGGACTGAGG TGAAGGAGTC AAAGAGTGTG CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT 
GAAAAAGATG GTATTCCAGC TAAAGAAGAG CTTTCAAATG AAAAGAGTGA TAAAAGAGCA 
GATGATGACA GTGCCATTAG ACAGGCTTCT GACCCTTCAG CAACATTGGA TAGTTCTGTC 
TCACTAACAC TTTCTGGACG AGAGTCTTCC TCCTGTAATG AAGTGCCAAT ATCACCAAAG 
CAAAGCCCCC GAGAGTCTCT TTCTTCACCA AAGCGGGCAG CCTCACCAAT CCATTTGCAG 
AATGATCAGC CCTTGCACAG TAACATCAGA CAAAGTAGTG CAGGGAAAGC AAAACCTGAA 
TGGTTCCTTC AGAAAGATTC AGAAGGGGAC ACACCATCTC TTATCAGCTG GCCCTCCAGA 
GTAAAAGTTA GAGAGAAACT GGTGAAAGAG GAAAGTGCAC GTGGAAGCCC TGAACCTGTC 
ACAGACAGTG TATCTCAGAG AAAATCCCAG CCAGTGTCAG CCCACTACAT GCCTCTTCAG 
ACAGAAACGT CTGCCTTTGA TAGGGTTCTC AATCAGCCCG TCAGTGCAGT CCGCCAACCA 
ATACATGGGT ATATTCAGCC TGCTGGTGTT GCACACACGG CTCAGCTGAT GGCAACAGAA 
GAACCAGAAA GCATCTTCGT TGGCAGCCGC CTCCTTCCAG ACAGTGTCAG CCCCTTAACG 
AAATCAGCAG CAGCCACTGC ACCAGAGGGT GAAAAGAAAG AGACGCTGGC TTATGGAGCA 
AAGCCTGGCG AAGAAGAATC CCCAGAGGGT GAGAAGGCTT CCAAAGGCAG AAGACCAATT 
TTGCCATCTG AGATTCGCAA GCAAGGGAAG AGCCACGAAG GCCTCTTTGG AGGAGAAGAA 
CCAGATACCA GTGTGGGGAG CTCCTCATCT GCAAGCAAGC CGAAGGTTGG CTCAGATGTT 
CAGCAGGAGC AGGAATGCAA AAAGAAGCAG GCGCCTGAGC AGCAGCTGAA GACACCTGAG 
AACATGGAGA GGAGCGAAGC TGTCATGTAC ATACAGAGCG GGTCTGTATC AAAGCCAGCC 
CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA AAGCATAAAG TCCTGACTCG CTCCATGTCT 
GACTATACTG TGCCTCCTAA GCTAGAAGCT TTTCATAGTA AGAGCATGGA AGCAAACGCA 
GCAAATGGTG AGATTGGCAT GGTTGACACA AAGGTCTCTG TTGCCCAGCT CCGTAATGTC 
TTTCTGGAAA CTGCTCATGC CAACAAGAAA ACGGACCTCG AATCTCGGTT TGAGATGTCA 
ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT GACCGTGAAA GAGGGCCGCG AAGGCCGAGG 
CGCTATTTCA CTCCTGGTGA AAATAGAAAG ACTTCTGAGC GATTTAAAAC CCAACCTATA 
ACGTCAGCAG AACGAAAGGA GACGGATAGG TCTATTATGT GTGCTGAAAT ACCAACTGCT 
GACGATGAAG AAAAATTAGA TGACCGAGCC AAACTAAGTG TAGCTGCAAA GAGACTGCTT 
TTTAGAGAAA TGGAAAAATC TTTTGAAATG AAGAATATTC CTAAACCACC TACGAGAAGC 
TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG CAGGACAGAT CACACACGCA GCCTATTACC 
AGTGAGGAAG TGGTCATTGC AGCTACTGAA ACTACCCCTG CTTCACGTTC TGTGAATACC 
CACACAGTAG TGGCAAGAGT ACCTAGTCCC ACTCTAGTTA AGAGCACTGT ACAACCTACC 
AGCTTGCAGG CATCAGCACA CCAAAAAATA CTAGCTAAAG AGGAGATAAG AGCAGCCAAA 
GAGGCGATGG GGCAAGATCA ATCCGATGAA CCAGATTCTT CCACATTAAG CTTGGCAGAA 
AAGATGGCTT TGTTTAACAA ACTGTCTCAG CCAGTATCCA GGGCTATATC CACAAGGAGT 
CGGGGAGATA TTAGGCACAG AAGGATGAAT ACTCGTTACC AGACCCAGCC AGTCACTCTT 
GGAGAAGTTG AGCAGATGCA ATGGAAAAAG AGTAAACCGG TGCAAAACGA AAGTGGAAAA 
CTAACTCCCC TGTCAAGTAC AGTTTCAACA TCAGTTTCAG CAATGGCTTC TGCCCTTTCT 
GCACTGTTTG CTGGAGACAT GCATGCGAAG TCACGTAGTG ATGGAAGTGT GAGCGCTGCC 
ACAAAAGATG AGTTGAGATT CCATGCTTCA GCAGAAAGCT CAGACTCATC AGGAAAACGC 
ACACAGAAGT CAGAACAGTG GCAGCCCTCA ATGGAAGTGC TGGAAAGCAA AAGAGCATCA 
AAGAAGCATG AAGAAGAAGG AAAGAGGTTT CTAGCACATG AGGCTAATGA AATCAGAAAG 
TACAGCTCCT TTGAGGATGA AACCACACAT CCTGTTCTTG AAAAAACAGG AGACTACCAT 
AAAGAGGCAG CCTACAGCTT CCTGAGGAAG GGCAGCATGG AACTGTTTAG TTCACAACCA 
CTTTTTCAGC CTCTTGAAAG GAAGGAAATT GATACCATCA TGCAAGACCA CGTGGAGCCT 
ACCTCTGAAC TCACCAGCAC ACCAGCAACG AGGACACTTT CCCAGACTAC AGCTGCAGCA 
TCCTGTAGGC TACAGGAGCT GTCTGAACAA CTGGAGGGAA AGTTCTACAA GAATTCCTGT 
GAGATGTTTT CTGCTGGAGA GAACAAAATA CAGACAACTG AGGATGCCAC CGATAGCTCC 
AGCAAAACGA TGTCAATTAA AGAAAGGTTG GCATTGCTGA AGAAAAGTGG TGAAGAAGAT 
TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA TATGCAAAAG TGTCTGCCAC TGATCGTAGC 
GCACAGATGC AAGAAGTTGA GCAGCTGTTG AAAAAGGAAC CTGTATATGC TTCTACTTAC 
TCTCCTGTTA TACCTGCTGT CCATAAATTT CATCCTTTTC TACCTATTAA TCAGCAGAGG 
ATAGCAGATA ATCAGGAGAG TCAAATGACT ATAGAAGAAA GAAAACACCT CATCACAGCT 
CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA GGCGCAGCAA ATGACTCCAC GCAGTTCACC 
GTAGCTGGTC GAATGGTAAA AAAAGGCCTG GCTTCTCCAA GTGCACTAAC ACCAGTAGCA 
TCCCCTTATG GTAACAGGCA GAAGTCTACA ACTCCGGTCA CAAAGCCCTT GGAAGAAATT 
GAAGCTAGGC CTGATATGCA ATTGGAATCA GATATGAAGC TTGACAAGCT GGAGTCATTC 
TTGGGAAGGC TGAATAACAA AGTCGCTGGA ATGCAAGAGA CAGTGTTGAC AGTTACAGGA 
AAATCTGTGA AAGAGGTGAT GAAGCTGGAT GATGATGAAA CATTTTCCAA ATTTTACCGC 
CGTGTGGATT TCCCTTCCTC CTCTGTGCCT TTGGACCTTG ATGAGGACTT TGATGCCATC 
TTTGACCCTT ATGCACCAAA GTTAATATCC TCTGTAGCAG AGCACAAACG TGCAGTGAGG 
CCTATGCGCA GGGTTCAGTC CTCCAGAAAC CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT 
ATTCTTCATG AGTACACTGA ACAAAGACTG AATGTTGCCT TCATGGAGTC AAAGCGAATG 
AAAGTAGAAA AAATGTCTGC AAACTCAAAT TTCTCAGAAG TAGCACTTGC TGGTTTGGCA 
AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG CGGAGTGTTA ACCTAACAGA GCAAAATTCT 
AACAACAGTG CTGTGCCATA CAAGAAGTTA ATGCTGCTGC AAATTAAAGG AAGAAGACAT 
GTTCAAACAA GATTAGTTGA ACCAAGGGCC TCATCACTGA ACAGTGGAGA CTGTTTCCTA 
TTGTTAACTC CACATCTCTG TTTCTTATGG GTAGGAGAGT TTGCAAATGT CATAGAAAAA 
GCAAAGGCTT CAGAACTTGC AACTTTGATT CAGACAAAGA GGGAACTTGG CTGTAGAGCT 
TCTTATATAC AGACTATAGA AGAAGGAATA AACACCCATA CCCATGCAGC CAAAGACTTC 
TGGAAACTCC TTGGTGGCCA AGCAAATTAC CAGTCTGCTG GAAGACCTGA AGAGGATGAA 
ATGTATGAAG CTGCCATAAT AGAAACTAAT TGCATTTACC GTCTTGTAGA GGATAAACTC 
ATTCCTGAAG ATGACTACTG GGGAAAGATG CCAAAATGTA CCCTGCTACA ACCAAAAGAG 
GTGCTCGTAT TTGATTTTGG CAGTGAAGTG TATGTCTGGC ATGGAAAAGA AGTTACTTTA 
GCACAAAGAA AAGTGGCATT CCAGCTGGCA AAACACTTAT GGAATGGCAC CTTCGACTAC 
TCAAATTGTG ACATAAATCC TCTGGACCCA GGAGAATGCA ATCCCCTCAT ACCCAGAAAG 
GGACAAGGAC GTCCAGACTG GGCTGTGTTT GGCAGGCTCA CAGAACATAA TGAGACCATA 
CTGTTCAAAG AAAAATTCCT TGATTGGACA GAGCTGAAGA AACTCAATGA GAAGAATTCT 
AGTGAATCCC TTCACCAGAA GGAAGAATCC AAATCTGATT CTAAACCATA TGATGTCATG 
CTAATGGTAC CTGTGCCCCA AACTGCTGTA GGCACGGTTC TGGATGGAAT GAATATTGGC 
CGGGGCTATG GACTTGTAGA AGGAGAAGAT GGGAGGCAGT TTGAAATTAT CACTGCATCT 
GTGGATGTCT GGCACATCCT GGAGTTTGAT TATAGTAGAT TGCCTAAACA AAGCATAGGA 
CAGTTCCATG AGGGAGACAC ATACGTGGTG AAGTGGAAGT ACATGGTGAG CACTACAGTT 
GGAAGCAGAC AAAAAGGAGA GCAACAAGTA AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT 
TTCTTTTGGC AAGGAAGGCA CTCCACAGTG AGTGAGAAAG GGACATCGGC ACTGATGACA 
GTGGAACTTG ATGAGGAGAG AGGAGCACAG GTACAAGTGC TTCAAGGGAA AGAACTACCA 
TGCTTCCTGC AGTGCTTCCA AGGAGGGATG ATTGTGCATG CTGGAAGAAG GGAAGAGGAA 
GAAGAAAATG CACAAAGTGA CTGGAGACTA TATTGCGTGC GAGGAGAAGT TCCCAATGAA 
GGAAACTTAC TTGAAGTAGC ATGTCACTGC AGCAGCCTGC GTTCCCGGAC ATCCATGATT 
GTCCTCAATA TAAATAAAGC TCTTATCTAC CTGTGGCATG GCTGCAAAGC ACAATCTCAC 
ACCAAGGATG TAGGAAGAAC AGCAGCCAAT AAAATAAAAG AACAATGTCC GCTGGAAGCA 
GGGCTGCACA GCAGCAGCAA GGTGACAATA CACGAATGTG ATGAAGGGTC AGAGCCTTTG 
GGATTCTGGG ATGCATTAGG AAGGCGAGAT CGAAAGGCCT ATGATTGCAT GTTGCAAGAT 
CCGGGAAAGT TTAATTTCAC CCCCCGCCTC TTCAGCCTCA GTAGTTCTTC GGGAGAATTC 
TCAGCCACTG AGTACGTTTA CCCTTCAAGA GACCCTACTG TCATCAATTC TATGCCCTTC 
TTGCAAGAGG ATCTTTACAC TGCCCCACAG CCAGCACTGT TCCTTGTTGA CAATCACCAT 
GAAGTGTACC TGTGGCAAGG CTGGTGGCCT GTGGAAAACA AAATCACTGG ATCAGCTCGA 
ATCAGGTGGG CTACAGACAG GAAATGCGCC ATGGAGACCG TGCTCCAGTA CTGCAAAGGA 
AAAAATGTAA AGAAGCCCCC AAAGTCCTAT CTCATTCATG CCGGCCTAGA GCCTCTGACC 
TTCACTAATA TGTTCCCAAG TTGGGAACAC AGGGAAGACA TTGCAGAGAT CACAGAAATG 
GATGCTGACG TTTCTAATCA GATAATCCTT GTAGAAGATG TGCTGGCCAA GCTGTGTAAA 
ACTGTGTATC CCCTAGCTGA TCTCTTAGCC AGGCCTTTAC CTGAGGGAGT TGATCCTCTG 
AAGCTTGAAA TTTATCTTTC AGATGAGGAC TTTGAGGTTG CATTAGAGAT GACAAGAGAA 
GAGTACAATG CGCTGCCATC TTGGAAGCAG GTTAATCTGA AAAAGGCAAA AGGACTTTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282094.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137580.1 Gallus gallus supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OGa31971
Clone ID Related Accession (Same CDS sequence)	XM_015282094.1
Accession Version	XM_015282094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGCAGAGGAT AGCAGATAAT 
CAGGAGAGTC AAATGACTAT AGAAGAAAGA AAACACCTCA TCACAGCTCG AGAAGAAGCC 
TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT GACTCCACGC AGTTCACCGT AGCTGGTCGA 
ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT GCACTAACAC CAGTAGCATC CCCTTATGGT 
AACAGGCAGA AGTCTACAAC TCCGGTCACA AAGCCCTTGG AAGAAATTGA AGCTAGGCCT 
GATATGCAAT TGGAATCAGA TATGAAGCTT GACAAGCTGG AGTCATTCTT GGGAAGGCTG 
AATAACAAAG TCGCTGGAAT GCAAGAGACA GTGTTGACAG TTACAGGAAA ATCTGTGAAA 
GAGGTGATGA AGCTGGATGA TGATGAAACA TTTTCCAAAT TTTACCGCCG TGTGGATTTC 
CCTTCCTCCT CTGTGCCTTT GGACCTTGAT GAGGACTTTG ATGCCATCTT TGACCCTTAT 
GCACCAAAGT TAATATCCTC TGTAGCAGAG CACAAACGTG CAGTGAGGCC TATGCGCAGG 
GTTCAGTCCT CCAGAAACCC CCTGAAAATG CTGGCAGCAA GAGAAGATAT TCTTCATGAG 
TACACTGAAC AAAGACTGAA TGTTGCCTTC ATGGAGTCAA AGCGAATGAA AGTAGAAAAA 
ATGTCTGCAA ACTCAAATTT CTCAGAAGTA GCACTTGCTG GTTTGGCAAG CAAGGAGAAC 
TTCAGCAACG TTAGTCTGCG GAGTGTTAAC CTAACAGAGC AAAATTCTAA CAACAGTGCT 
GTGCCATACA AGAAGTTAAT GCTGCTGCAA ATTAAAGGAA GAAGACATGT TCAAACAAGA 
TTAGTTGAAC CAAGGGCCTC ATCACTGAAC AGTGGAGACT GTTTCCTATT GTTAACTCCA 
CATCTCTGTT TCTTATGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAAGC AAAGGCTTCA 
GAACTTGCAA CTTTGATTCA GACAAAGAGG GAACTTGGCT GTAGAGCTTC TTATATACAG 
ACTATAGAAG AAGGAATAAA CACCCATACC CATGCAGCCA AAGACTTCTG GAAACTCCTT 
GGTGGCCAAG CAAATTACCA GTCTGCTGGA AGACCTGAAG AGGATGAAAT GTATGAAGCT 
GCCATAATAG AAACTAATTG CATTTACCGT CTTGTAGAGG ATAAACTCAT TCCTGAAGAT 
GACTACTGGG GAAAGATGCC AAAATGTACC CTGCTACAAC CAAAAGAGGT GCTCGTATTT 
GATTTTGGCA GTGAAGTGTA TGTCTGGCAT GGAAAAGAAG TTACTTTAGC ACAAAGAAAA 
GTGGCATTCC AGCTGGCAAA ACACTTATGG AATGGCACCT TCGACTACTC AAATTGTGAC 
ATAAATCCTC TGGACCCAGG AGAATGCAAT CCCCTCATAC CCAGAAAGGG ACAAGGACGT 
CCAGACTGGG CTGTGTTTGG CAGGCTCACA GAACATAATG AGACCATACT GTTCAAAGAA 
AAATTCCTTG ATTGGACAGA GCTGAAGAAA CTCAATGAGA AGAATTCTAG TGAATCCCTT 
CACCAGAAGG AAGAATCCAA ATCTGATTCT AAACCATATG ATGTCATGCT AATGGTACCT 
GTGCCCCAAA CTGCTGTAGG CACGGTTCTG GATGGAATGA ATATTGGCCG GGGCTATGGA 
CTTGTAGAAG GAGAAGATGG GAGGCAGTTT GAAATTATCA CTGCATCTGT GGATGTCTGG 
CACATCCTGG AGTTTGATTA TAGTAGATTG CCTAAACAAA GCATAGGACA GTTCCATGAG 
GGAGACACAT ACGTGGTGAA GTGGAAGTAC ATGGTGAGCA CTACAGTTGG AAGCAGACAA 
AAAGGAGAGC AACAAGTAAG AGCTGTTGGA AAAGAGAAAT GTGTGTATTT CTTTTGGCAA 
GGAAGGCACT CCACAGTGAG TGAGAAAGGG ACATCGGCAC TGATGACAGT GGAACTTGAT 
GAGGAGAGAG GAGCACAGGT ACAAGTGCTT CAAGGGAAAG AACTACCATG CTTCCTGCAG 
TGCTTCCAAG GAGGGATGAT TGTGCATGCT GGAAGAAGGG AAGAGGAAGA AGAAAATGCA 
CAAAGTGACT GGAGACTATA TTGCGTGCGA GGAGAAGTTC CCAATGAAGG AAACTTACTT 
GAAGTAGCAT GTCACTGCAG CAGCCTGCGT TCCCGGACAT CCATGATTGT CCTCAATATA 
AATAAAGCTC TTATCTACCT GTGGCATGGC TGCAAAGCAC AATCTCACAC CAAGGATGTA 
GGAAGAACAG CAGCCAATAA AATAAAAGAA CAATGTCCGC TGGAAGCAGG GCTGCACAGC 
AGCAGCAAGG TGACAATACA CGAATGTGAT GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT 
GCATTAGGAA GGCGAGATCG AAAGGCCTAT GATTGCATGT TGCAAGATCC GGGAAAGTTT 
AATTTCACCC CCCGCCTCTT CAGCCTCAGT AGTTCTTCGG GAGAATTCTC AGCCACTGAG 
TACGTTTACC CTTCAAGAGA CCCTACTGTC ATCAATTCTA TGCCCTTCTT GCAAGAGGAT 
CTTTACACTG CCCCACAGCC AGCACTGTTC CTTGTTGACA ATCACCATGA AGTGTACCTG 
TGGCAAGGCT GGTGGCCTGT GGAAAACAAA ATCACTGGAT CAGCTCGAAT CAGGTGGGCT 
ACAGACAGGA AATGCGCCAT GGAGACCGTG CTCCAGTACT GCAAAGGAAA AAATGTAAAG 
AAGCCCCCAA AGTCCTATCT CATTCATGCC GGCCTAGAGC CTCTGACCTT CACTAATATG 
TTCCCAAGTT GGGAACACAG GGAAGACATT GCAGAGATCA CAGAAATGGA TGCTGACGTT 
TCTAATCAGA TAATCCTTGT AGAAGATGTG CTGGCCAAGC TGTGTAAAAC TGTGTATCCC 
CTAGCTGATC TCTTAGCCAG GCCTTTACCT GAGGGAGTTG ATCCTCTGAA GCTTGAAATT 
TATCTTTCAG ATGAGGACTT TGAGGTTGCA TTAGAGATGA CAAGAGAAGA GTACAATGCG 
CTGCCATCTT GGAAGCAGGT TAATCTGAAA AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137580.1
CDS	17..6907
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282094.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282094.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGCAGAGGAT AGCAGATAAT 
CAGGAGAGTC AAATGACTAT AGAAGAAAGA AAACACCTCA TCACAGCTCG AGAAGAAGCC 
TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT GACTCCACGC AGTTCACCGT AGCTGGTCGA 
ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT GCACTAACAC CAGTAGCATC CCCTTATGGT 
AACAGGCAGA AGTCTACAAC TCCGGTCACA AAGCCCTTGG AAGAAATTGA AGCTAGGCCT 
GATATGCAAT TGGAATCAGA TATGAAGCTT GACAAGCTGG AGTCATTCTT GGGAAGGCTG 
AATAACAAAG TCGCTGGAAT GCAAGAGACA GTGTTGACAG TTACAGGAAA ATCTGTGAAA 
GAGGTGATGA AGCTGGATGA TGATGAAACA TTTTCCAAAT TTTACCGCCG TGTGGATTTC 
CCTTCCTCCT CTGTGCCTTT GGACCTTGAT GAGGACTTTG ATGCCATCTT TGACCCTTAT 
GCACCAAAGT TAATATCCTC TGTAGCAGAG CACAAACGTG CAGTGAGGCC TATGCGCAGG 
GTTCAGTCCT CCAGAAACCC CCTGAAAATG CTGGCAGCAA GAGAAGATAT TCTTCATGAG 
TACACTGAAC AAAGACTGAA TGTTGCCTTC ATGGAGTCAA AGCGAATGAA AGTAGAAAAA 
ATGTCTGCAA ACTCAAATTT CTCAGAAGTA GCACTTGCTG GTTTGGCAAG CAAGGAGAAC 
TTCAGCAACG TTAGTCTGCG GAGTGTTAAC CTAACAGAGC AAAATTCTAA CAACAGTGCT 
GTGCCATACA AGAAGTTAAT GCTGCTGCAA ATTAAAGGAA GAAGACATGT TCAAACAAGA 
TTAGTTGAAC CAAGGGCCTC ATCACTGAAC AGTGGAGACT GTTTCCTATT GTTAACTCCA 
CATCTCTGTT TCTTATGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAAGC AAAGGCTTCA 
GAACTTGCAA CTTTGATTCA GACAAAGAGG GAACTTGGCT GTAGAGCTTC TTATATACAG 
ACTATAGAAG AAGGAATAAA CACCCATACC CATGCAGCCA AAGACTTCTG GAAACTCCTT 
GGTGGCCAAG CAAATTACCA GTCTGCTGGA AGACCTGAAG AGGATGAAAT GTATGAAGCT 
GCCATAATAG AAACTAATTG CATTTACCGT CTTGTAGAGG ATAAACTCAT TCCTGAAGAT 
GACTACTGGG GAAAGATGCC AAAATGTACC CTGCTACAAC CAAAAGAGGT GCTCGTATTT 
GATTTTGGCA GTGAAGTGTA TGTCTGGCAT GGAAAAGAAG TTACTTTAGC ACAAAGAAAA 
GTGGCATTCC AGCTGGCAAA ACACTTATGG AATGGCACCT TCGACTACTC AAATTGTGAC 
ATAAATCCTC TGGACCCAGG AGAATGCAAT CCCCTCATAC CCAGAAAGGG ACAAGGACGT 
CCAGACTGGG CTGTGTTTGG CAGGCTCACA GAACATAATG AGACCATACT GTTCAAAGAA 
AAATTCCTTG ATTGGACAGA GCTGAAGAAA CTCAATGAGA AGAATTCTAG TGAATCCCTT 
CACCAGAAGG AAGAATCCAA ATCTGATTCT AAACCATATG ATGTCATGCT AATGGTACCT 
GTGCCCCAAA CTGCTGTAGG CACGGTTCTG GATGGAATGA ATATTGGCCG GGGCTATGGA 
CTTGTAGAAG GAGAAGATGG GAGGCAGTTT GAAATTATCA CTGCATCTGT GGATGTCTGG 
CACATCCTGG AGTTTGATTA TAGTAGATTG CCTAAACAAA GCATAGGACA GTTCCATGAG 
GGAGACACAT ACGTGGTGAA GTGGAAGTAC ATGGTGAGCA CTACAGTTGG AAGCAGACAA 
AAAGGAGAGC AACAAGTAAG AGCTGTTGGA AAAGAGAAAT GTGTGTATTT CTTTTGGCAA 
GGAAGGCACT CCACAGTGAG TGAGAAAGGG ACATCGGCAC TGATGACAGT GGAACTTGAT 
GAGGAGAGAG GAGCACAGGT ACAAGTGCTT CAAGGGAAAG AACTACCATG CTTCCTGCAG 
TGCTTCCAAG GAGGGATGAT TGTGCATGCT GGAAGAAGGG AAGAGGAAGA AGAAAATGCA 
CAAAGTGACT GGAGACTATA TTGCGTGCGA GGAGAAGTTC CCAATGAAGG AAACTTACTT 
GAAGTAGCAT GTCACTGCAG CAGCCTGCGT TCCCGGACAT CCATGATTGT CCTCAATATA 
AATAAAGCTC TTATCTACCT GTGGCATGGC TGCAAAGCAC AATCTCACAC CAAGGATGTA 
GGAAGAACAG CAGCCAATAA AATAAAAGAA CAATGTCCGC TGGAAGCAGG GCTGCACAGC 
AGCAGCAAGG TGACAATACA CGAATGTGAT GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT 
GCATTAGGAA GGCGAGATCG AAAGGCCTAT GATTGCATGT TGCAAGATCC GGGAAAGTTT 
AATTTCACCC CCCGCCTCTT CAGCCTCAGT AGTTCTTCGG GAGAATTCTC AGCCACTGAG 
TACGTTTACC CTTCAAGAGA CCCTACTGTC ATCAATTCTA TGCCCTTCTT GCAAGAGGAT 
CTTTACACTG CCCCACAGCC AGCACTGTTC CTTGTTGACA ATCACCATGA AGTGTACCTG 
TGGCAAGGCT GGTGGCCTGT GGAAAACAAA ATCACTGGAT CAGCTCGAAT CAGGTGGGCT 
ACAGACAGGA AATGCGCCAT GGAGACCGTG CTCCAGTACT GCAAAGGAAA AAATGTAAAG 
AAGCCCCCAA AGTCCTATCT CATTCATGCC GGCCTAGAGC CTCTGACCTT CACTAATATG 
TTCCCAAGTT GGGAACACAG GGAAGACATT GCAGAGATCA CAGAAATGGA TGCTGACGTT 
TCTAATCAGA TAATCCTTGT AGAAGATGTG CTGGCCAAGC TGTGTAAAAC TGTGTATCCC 
CTAGCTGATC TCTTAGCCAG GCCTTTACCT GAGGGAGTTG ATCCTCTGAA GCTTGAAATT 
TATCTTTCAG ATGAGGACTT TGAGGTTGCA TTAGAGATGA CAAGAGAAGA GTACAATGCG 
CTGCCATCTT GGAAGCAGGT TAATCTGAAA AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282095.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137581.1 Gallus gallus supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OGa31972
Clone ID Related Accession (Same CDS sequence)	XM_015282095.1 , XM_015282095.2
Accession Version	XM_015282095.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGAG GATAGCAGAT 
AATCAGGAGA GTCAAATGAC TATAGAAGAA AGAAAACACC TCATCACAGC TCGAGAAGAA 
GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA AATGACTCCA CGCAGTTCAC CGTAGCTGGT 
CGAATGGTAA AAAAAGGCCT GGCTTCTCCA AGTGCACTAA CACCAGTAGC ATCCCCTTAT 
GGTAACAGGC AGAAGTCTAC AACTCCGGTC ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG 
CCTGATATGC AATTGGAATC AGATATGAAG CTTGACAAGC TGGAGTCATT CTTGGGAAGG 
CTGAATAACA AAGTCGCTGG AATGCAAGAG ACAGTGTTGA CAGTTACAGG AAAATCTGTG 
AAAGAGGTGA TGAAGCTGGA TGATGATGAA ACATTTTCCA AATTTTACCG CCGTGTGGAT 
TTCCCTTCCT CCTCTGTGCC TTTGGACCTT GATGAGGACT TTGATGCCAT CTTTGACCCT 
TATGCACCAA AGTTAATATC CTCTGTAGCA GAGCACAAAC GTGCAGTGAG GCCTATGCGC 
AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA ATGCTGGCAG CAAGAGAAGA TATTCTTCAT 
GAGTACACTG AACAAAGACT GAATGTTGCC TTCATGGAGT CAAAGCGAAT GAAAGTAGAA 
AAAATGTCTG CAAACTCAAA TTTCTCAGAA GTAGCACTTG CTGGTTTGGC AAGCAAGGAG 
AACTTCAGCA ACGTTAGTCT GCGGAGTGTT AACCTAACAG AGCAAAATTC TAACAACAGT 
GCTGTGCCAT ACAAGAAGTT AATGCTGCTG CAAATTAAAG GAAGAAGACA TGTTCAAACA 
AGATTAGTTG AACCAAGGGC CTCATCACTG AACAGTGGAG ACTGTTTCCT ATTGTTAACT 
CCACATCTCT GTTTCTTATG GGTAGGAGAG TTTGCAAATG TCATAGAAAA AGCAAAGGCT 
TCAGAACTTG CAACTTTGAT TCAGACAAAG AGGGAACTTG GCTGTAGAGC TTCTTATATA 
CAGACTATAG AAGAAGGAAT AAACACCCAT ACCCATGCAG CCAAAGACTT CTGGAAACTC 
CTTGGTGGCC AAGCAAATTA CCAGTCTGCT GGAAGACCTG AAGAGGATGA AATGTATGAA 
GCTGCCATAA TAGAAACTAA TTGCATTTAC CGTCTTGTAG AGGATAAACT CATTCCTGAA 
GATGACTACT GGGGAAAGAT GCCAAAATGT ACCCTGCTAC AACCAAAAGA GGTGCTCGTA 
TTTGATTTTG GCAGTGAAGT GTATGTCTGG CATGGAAAAG AAGTTACTTT AGCACAAAGA 
AAAGTGGCAT TCCAGCTGGC AAAACACTTA TGGAATGGCA CCTTCGACTA CTCAAATTGT 
GACATAAATC CTCTGGACCC AGGAGAATGC AATCCCCTCA TACCCAGAAA GGGACAAGGA 
CGTCCAGACT GGGCTGTGTT TGGCAGGCTC ACAGAACATA ATGAGACCAT ACTGTTCAAA 
GAAAAATTCC TTGATTGGAC AGAGCTGAAG AAACTCAATG AGAAGAATTC TAGTGAATCC 
CTTCACCAGA AGGAAGAATC CAAATCTGAT TCTAAACCAT ATGATGTCAT GCTAATGGTA 
CCTGTGCCCC AAACTGCTGT AGGCACGGTT CTGGATGGAA TGAATATTGG CCGGGGCTAT 
GGACTTGTAG AAGGAGAAGA TGGGAGGCAG TTTGAAATTA TCACTGCATC TGTGGATGTC 
TGGCACATCC TGGAGTTTGA TTATAGTAGA TTGCCTAAAC AAAGCATAGG ACAGTTCCAT 
GAGGGAGACA CATACGTGGT GAAGTGGAAG TACATGGTGA GCACTACAGT TGGAAGCAGA 
CAAAAAGGAG AGCAACAAGT AAGAGCTGTT GGAAAAGAGA AATGTGTGTA TTTCTTTTGG 
CAAGGAAGGC ACTCCACAGT GAGTGAGAAA GGGACATCGG CACTGATGAC AGTGGAACTT 
GATGAGGAGA GAGGAGCACA GGTACAAGTG CTTCAAGGGA AAGAACTACC ATGCTTCCTG 
CAGTGCTTCC AAGGAGGGAT GATTGTGCAT GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT 
GCACAAAGTG ACTGGAGACT ATATTGCGTG CGAGGAGAAG TTCCCAATGA AGGAAACTTA 
CTTGAAGTAG CATGTCACTG CAGCAGCCTG CGTTCCCGGA CATCCATGAT TGTCCTCAAT 
ATAAATAAAG CTCTTATCTA CCTGTGGCAT GGCTGCAAAG CACAATCTCA CACCAAGGAT 
GTAGGAAGAA CAGCAGCCAA TAAAATAAAA GAACAATGTC CGCTGGAAGC AGGGCTGCAC 
AGCAGCAGCA AGGTGACAAT ACACGAATGT GATGAAGGGT CAGAGCCTTT GGGATTCTGG 
GATGCATTAG GAAGGCGAGA TCGAAAGGCC TATGATTGCA TGTTGCAAGA TCCGGGAAAG 
TTTAATTTCA CCCCCCGCCT CTTCAGCCTC AGTAGTTCTT CGGGAGAATT CTCAGCCACT 
GAGTACGTTT ACCCTTCAAG AGACCCTACT GTCATCAATT CTATGCCCTT CTTGCAAGAG 
GATCTTTACA CTGCCCCACA GCCAGCACTG TTCCTTGTTG ACAATCACCA TGAAGTGTAC 
CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC AAAATCACTG GATCAGCTCG AATCAGGTGG 
GCTACAGACA GGAAATGCGC CATGGAGACC GTGCTCCAGT ACTGCAAAGG AAAAAATGTA 
AAGAAGCCCC CAAAGTCCTA TCTCATTCAT GCCGGCCTAG AGCCTCTGAC CTTCACTAAT 
ATGTTCCCAA GTTGGGAACA CAGGGAAGAC ATTGCAGAGA TCACAGAAAT GGATGCTGAC 
GTTTCTAATC AGATAATCCT TGTAGAAGAT GTGCTGGCCA AGCTGTGTAA AACTGTGTAT 
CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA CCTGAGGGAG TTGATCCTCT GAAGCTTGAA 
ATTTATCTTT CAGATGAGGA CTTTGAGGTT GCATTAGAGA TGACAAGAGA AGAGTACAAT 
GCGCTGCCAT CTTGGAAGCA GGTTAATCTG AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137581.1
CDS	17..6850
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282095.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282095.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGAG GATAGCAGAT 
AATCAGGAGA GTCAAATGAC TATAGAAGAA AGAAAACACC TCATCACAGC TCGAGAAGAA 
GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA AATGACTCCA CGCAGTTCAC CGTAGCTGGT 
CGAATGGTAA AAAAAGGCCT GGCTTCTCCA AGTGCACTAA CACCAGTAGC ATCCCCTTAT 
GGTAACAGGC AGAAGTCTAC AACTCCGGTC ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG 
CCTGATATGC AATTGGAATC AGATATGAAG CTTGACAAGC TGGAGTCATT CTTGGGAAGG 
CTGAATAACA AAGTCGCTGG AATGCAAGAG ACAGTGTTGA CAGTTACAGG AAAATCTGTG 
AAAGAGGTGA TGAAGCTGGA TGATGATGAA ACATTTTCCA AATTTTACCG CCGTGTGGAT 
TTCCCTTCCT CCTCTGTGCC TTTGGACCTT GATGAGGACT TTGATGCCAT CTTTGACCCT 
TATGCACCAA AGTTAATATC CTCTGTAGCA GAGCACAAAC GTGCAGTGAG GCCTATGCGC 
AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA ATGCTGGCAG CAAGAGAAGA TATTCTTCAT 
GAGTACACTG AACAAAGACT GAATGTTGCC TTCATGGAGT CAAAGCGAAT GAAAGTAGAA 
AAAATGTCTG CAAACTCAAA TTTCTCAGAA GTAGCACTTG CTGGTTTGGC AAGCAAGGAG 
AACTTCAGCA ACGTTAGTCT GCGGAGTGTT AACCTAACAG AGCAAAATTC TAACAACAGT 
GCTGTGCCAT ACAAGAAGTT AATGCTGCTG CAAATTAAAG GAAGAAGACA TGTTCAAACA 
AGATTAGTTG AACCAAGGGC CTCATCACTG AACAGTGGAG ACTGTTTCCT ATTGTTAACT 
CCACATCTCT GTTTCTTATG GGTAGGAGAG TTTGCAAATG TCATAGAAAA AGCAAAGGCT 
TCAGAACTTG CAACTTTGAT TCAGACAAAG AGGGAACTTG GCTGTAGAGC TTCTTATATA 
CAGACTATAG AAGAAGGAAT AAACACCCAT ACCCATGCAG CCAAAGACTT CTGGAAACTC 
CTTGGTGGCC AAGCAAATTA CCAGTCTGCT GGAAGACCTG AAGAGGATGA AATGTATGAA 
GCTGCCATAA TAGAAACTAA TTGCATTTAC CGTCTTGTAG AGGATAAACT CATTCCTGAA 
GATGACTACT GGGGAAAGAT GCCAAAATGT ACCCTGCTAC AACCAAAAGA GGTGCTCGTA 
TTTGATTTTG GCAGTGAAGT GTATGTCTGG CATGGAAAAG AAGTTACTTT AGCACAAAGA 
AAAGTGGCAT TCCAGCTGGC AAAACACTTA TGGAATGGCA CCTTCGACTA CTCAAATTGT 
GACATAAATC CTCTGGACCC AGGAGAATGC AATCCCCTCA TACCCAGAAA GGGACAAGGA 
CGTCCAGACT GGGCTGTGTT TGGCAGGCTC ACAGAACATA ATGAGACCAT ACTGTTCAAA 
GAAAAATTCC TTGATTGGAC AGAGCTGAAG AAACTCAATG AGAAGAATTC TAGTGAATCC 
CTTCACCAGA AGGAAGAATC CAAATCTGAT TCTAAACCAT ATGATGTCAT GCTAATGGTA 
CCTGTGCCCC AAACTGCTGT AGGCACGGTT CTGGATGGAA TGAATATTGG CCGGGGCTAT 
GGACTTGTAG AAGGAGAAGA TGGGAGGCAG TTTGAAATTA TCACTGCATC TGTGGATGTC 
TGGCACATCC TGGAGTTTGA TTATAGTAGA TTGCCTAAAC AAAGCATAGG ACAGTTCCAT 
GAGGGAGACA CATACGTGGT GAAGTGGAAG TACATGGTGA GCACTACAGT TGGAAGCAGA 
CAAAAAGGAG AGCAACAAGT AAGAGCTGTT GGAAAAGAGA AATGTGTGTA TTTCTTTTGG 
CAAGGAAGGC ACTCCACAGT GAGTGAGAAA GGGACATCGG CACTGATGAC AGTGGAACTT 
GATGAGGAGA GAGGAGCACA GGTACAAGTG CTTCAAGGGA AAGAACTACC ATGCTTCCTG 
CAGTGCTTCC AAGGAGGGAT GATTGTGCAT GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT 
GCACAAAGTG ACTGGAGACT ATATTGCGTG CGAGGAGAAG TTCCCAATGA AGGAAACTTA 
CTTGAAGTAG CATGTCACTG CAGCAGCCTG CGTTCCCGGA CATCCATGAT TGTCCTCAAT 
ATAAATAAAG CTCTTATCTA CCTGTGGCAT GGCTGCAAAG CACAATCTCA CACCAAGGAT 
GTAGGAAGAA CAGCAGCCAA TAAAATAAAA GAACAATGTC CGCTGGAAGC AGGGCTGCAC 
AGCAGCAGCA AGGTGACAAT ACACGAATGT GATGAAGGGT CAGAGCCTTT GGGATTCTGG 
GATGCATTAG GAAGGCGAGA TCGAAAGGCC TATGATTGCA TGTTGCAAGA TCCGGGAAAG 
TTTAATTTCA CCCCCCGCCT CTTCAGCCTC AGTAGTTCTT CGGGAGAATT CTCAGCCACT 
GAGTACGTTT ACCCTTCAAG AGACCCTACT GTCATCAATT CTATGCCCTT CTTGCAAGAG 
GATCTTTACA CTGCCCCACA GCCAGCACTG TTCCTTGTTG ACAATCACCA TGAAGTGTAC 
CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC AAAATCACTG GATCAGCTCG AATCAGGTGG 
GCTACAGACA GGAAATGCGC CATGGAGACC GTGCTCCAGT ACTGCAAAGG AAAAAATGTA 
AAGAAGCCCC CAAAGTCCTA TCTCATTCAT GCCGGCCTAG AGCCTCTGAC CTTCACTAAT 
ATGTTCCCAA GTTGGGAACA CAGGGAAGAC ATTGCAGAGA TCACAGAAAT GGATGCTGAC 
GTTTCTAATC AGATAATCCT TGTAGAAGAT GTGCTGGCCA AGCTGTGTAA AACTGTGTAT 
CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA CCTGAGGGAG TTGATCCTCT GAAGCTTGAA 
ATTTATCTTT CAGATGAGGA CTTTGAGGTT GCATTAGAGA TGACAAGAGA AGAGTACAAT 
GCGCTGCCAT CTTGGAAGCA GGTTAATCTG AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282095.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137581.1 Gallus gallus supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OGa31972
Clone ID Related Accession (Same CDS sequence)	XM_015282095.1 , XM_015282095.2
Accession Version	XM_015282095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGAG GATAGCAGAT 
AATCAGGAGA GTCAAATGAC TATAGAAGAA AGAAAACACC TCATCACAGC TCGAGAAGAA 
GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA AATGACTCCA CGCAGTTCAC CGTAGCTGGT 
CGAATGGTAA AAAAAGGCCT GGCTTCTCCA AGTGCACTAA CACCAGTAGC ATCCCCTTAT 
GGTAACAGGC AGAAGTCTAC AACTCCGGTC ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG 
CCTGATATGC AATTGGAATC AGATATGAAG CTTGACAAGC TGGAGTCATT CTTGGGAAGG 
CTGAATAACA AAGTCGCTGG AATGCAAGAG ACAGTGTTGA CAGTTACAGG AAAATCTGTG 
AAAGAGGTGA TGAAGCTGGA TGATGATGAA ACATTTTCCA AATTTTACCG CCGTGTGGAT 
TTCCCTTCCT CCTCTGTGCC TTTGGACCTT GATGAGGACT TTGATGCCAT CTTTGACCCT 
TATGCACCAA AGTTAATATC CTCTGTAGCA GAGCACAAAC GTGCAGTGAG GCCTATGCGC 
AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA ATGCTGGCAG CAAGAGAAGA TATTCTTCAT 
GAGTACACTG AACAAAGACT GAATGTTGCC TTCATGGAGT CAAAGCGAAT GAAAGTAGAA 
AAAATGTCTG CAAACTCAAA TTTCTCAGAA GTAGCACTTG CTGGTTTGGC AAGCAAGGAG 
AACTTCAGCA ACGTTAGTCT GCGGAGTGTT AACCTAACAG AGCAAAATTC TAACAACAGT 
GCTGTGCCAT ACAAGAAGTT AATGCTGCTG CAAATTAAAG GAAGAAGACA TGTTCAAACA 
AGATTAGTTG AACCAAGGGC CTCATCACTG AACAGTGGAG ACTGTTTCCT ATTGTTAACT 
CCACATCTCT GTTTCTTATG GGTAGGAGAG TTTGCAAATG TCATAGAAAA AGCAAAGGCT 
TCAGAACTTG CAACTTTGAT TCAGACAAAG AGGGAACTTG GCTGTAGAGC TTCTTATATA 
CAGACTATAG AAGAAGGAAT AAACACCCAT ACCCATGCAG CCAAAGACTT CTGGAAACTC 
CTTGGTGGCC AAGCAAATTA CCAGTCTGCT GGAAGACCTG AAGAGGATGA AATGTATGAA 
GCTGCCATAA TAGAAACTAA TTGCATTTAC CGTCTTGTAG AGGATAAACT CATTCCTGAA 
GATGACTACT GGGGAAAGAT GCCAAAATGT ACCCTGCTAC AACCAAAAGA GGTGCTCGTA 
TTTGATTTTG GCAGTGAAGT GTATGTCTGG CATGGAAAAG AAGTTACTTT AGCACAAAGA 
AAAGTGGCAT TCCAGCTGGC AAAACACTTA TGGAATGGCA CCTTCGACTA CTCAAATTGT 
GACATAAATC CTCTGGACCC AGGAGAATGC AATCCCCTCA TACCCAGAAA GGGACAAGGA 
CGTCCAGACT GGGCTGTGTT TGGCAGGCTC ACAGAACATA ATGAGACCAT ACTGTTCAAA 
GAAAAATTCC TTGATTGGAC AGAGCTGAAG AAACTCAATG AGAAGAATTC TAGTGAATCC 
CTTCACCAGA AGGAAGAATC CAAATCTGAT TCTAAACCAT ATGATGTCAT GCTAATGGTA 
CCTGTGCCCC AAACTGCTGT AGGCACGGTT CTGGATGGAA TGAATATTGG CCGGGGCTAT 
GGACTTGTAG AAGGAGAAGA TGGGAGGCAG TTTGAAATTA TCACTGCATC TGTGGATGTC 
TGGCACATCC TGGAGTTTGA TTATAGTAGA TTGCCTAAAC AAAGCATAGG ACAGTTCCAT 
GAGGGAGACA CATACGTGGT GAAGTGGAAG TACATGGTGA GCACTACAGT TGGAAGCAGA 
CAAAAAGGAG AGCAACAAGT AAGAGCTGTT GGAAAAGAGA AATGTGTGTA TTTCTTTTGG 
CAAGGAAGGC ACTCCACAGT GAGTGAGAAA GGGACATCGG CACTGATGAC AGTGGAACTT 
GATGAGGAGA GAGGAGCACA GGTACAAGTG CTTCAAGGGA AAGAACTACC ATGCTTCCTG 
CAGTGCTTCC AAGGAGGGAT GATTGTGCAT GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT 
GCACAAAGTG ACTGGAGACT ATATTGCGTG CGAGGAGAAG TTCCCAATGA AGGAAACTTA 
CTTGAAGTAG CATGTCACTG CAGCAGCCTG CGTTCCCGGA CATCCATGAT TGTCCTCAAT 
ATAAATAAAG CTCTTATCTA CCTGTGGCAT GGCTGCAAAG CACAATCTCA CACCAAGGAT 
GTAGGAAGAA CAGCAGCCAA TAAAATAAAA GAACAATGTC CGCTGGAAGC AGGGCTGCAC 
AGCAGCAGCA AGGTGACAAT ACACGAATGT GATGAAGGGT CAGAGCCTTT GGGATTCTGG 
GATGCATTAG GAAGGCGAGA TCGAAAGGCC TATGATTGCA TGTTGCAAGA TCCGGGAAAG 
TTTAATTTCA CCCCCCGCCT CTTCAGCCTC AGTAGTTCTT CGGGAGAATT CTCAGCCACT 
GAGTACGTTT ACCCTTCAAG AGACCCTACT GTCATCAATT CTATGCCCTT CTTGCAAGAG 
GATCTTTACA CTGCCCCACA GCCAGCACTG TTCCTTGTTG ACAATCACCA TGAAGTGTAC 
CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC AAAATCACTG GATCAGCTCG AATCAGGTGG 
GCTACAGACA GGAAATGCGC CATGGAGACC GTGCTCCAGT ACTGCAAAGG AAAAAATGTA 
AAGAAGCCCC CAAAGTCCTA TCTCATTCAT GCCGGCCTAG AGCCTCTGAC CTTCACTAAT 
ATGTTCCCAA GTTGGGAACA CAGGGAAGAC ATTGCAGAGA TCACAGAAAT GGATGCTGAC 
GTTTCTAATC AGATAATCCT TGTAGAAGAT GTGCTGGCCA AGCTGTGTAA AACTGTGTAT 
CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA CCTGAGGGAG TTGATCCTCT GAAGCTTGAA 
ATTTATCTTT CAGATGAGGA CTTTGAGGTT GCATTAGAGA TGACAAGAGA AGAGTACAAT 
GCGCTGCCAT CTTGGAAGCA GGTTAATCTG AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137581.1
CDS	19..6852
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282095.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282095.2

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGAG GATAGCAGAT 
AATCAGGAGA GTCAAATGAC TATAGAAGAA AGAAAACACC TCATCACAGC TCGAGAAGAA 
GCCTGGAAGT CCAGAGGTAA AGGCGCAGCA AATGACTCCA CGCAGTTCAC CGTAGCTGGT 
CGAATGGTAA AAAAAGGCCT GGCTTCTCCA AGTGCACTAA CACCAGTAGC ATCCCCTTAT 
GGTAACAGGC AGAAGTCTAC AACTCCGGTC ACAAAGCCCT TGGAAGAAAT TGAAGCTAGG 
CCTGATATGC AATTGGAATC AGATATGAAG CTTGACAAGC TGGAGTCATT CTTGGGAAGG 
CTGAATAACA AAGTCGCTGG AATGCAAGAG ACAGTGTTGA CAGTTACAGG AAAATCTGTG 
AAAGAGGTGA TGAAGCTGGA TGATGATGAA ACATTTTCCA AATTTTACCG CCGTGTGGAT 
TTCCCTTCCT CCTCTGTGCC TTTGGACCTT GATGAGGACT TTGATGCCAT CTTTGACCCT 
TATGCACCAA AGTTAATATC CTCTGTAGCA GAGCACAAAC GTGCAGTGAG GCCTATGCGC 
AGGGTTCAGT CCTCCAGAAA CCCCCTGAAA ATGCTGGCAG CAAGAGAAGA TATTCTTCAT 
GAGTACACTG AACAAAGACT GAATGTTGCC TTCATGGAGT CAAAGCGAAT GAAAGTAGAA 
AAAATGTCTG CAAACTCAAA TTTCTCAGAA GTAGCACTTG CTGGTTTGGC AAGCAAGGAG 
AACTTCAGCA ACGTTAGTCT GCGGAGTGTT AACCTAACAG AGCAAAATTC TAACAACAGT 
GCTGTGCCAT ACAAGAAGTT AATGCTGCTG CAAATTAAAG GAAGAAGACA TGTTCAAACA 
AGATTAGTTG AACCAAGGGC CTCATCACTG AACAGTGGAG ACTGTTTCCT ATTGTTAACT 
CCACATCTCT GTTTCTTATG GGTAGGAGAG TTTGCAAATG TCATAGAAAA AGCAAAGGCT 
TCAGAACTTG CAACTTTGAT TCAGACAAAG AGGGAACTTG GCTGTAGAGC TTCTTATATA 
CAGACTATAG AAGAAGGAAT AAACACCCAT ACCCATGCAG CCAAAGACTT CTGGAAACTC 
CTTGGTGGCC AAGCAAATTA CCAGTCTGCT GGAAGACCTG AAGAGGATGA AATGTATGAA 
GCTGCCATAA TAGAAACTAA TTGCATTTAC CGTCTTGTAG AGGATAAACT CATTCCTGAA 
GATGACTACT GGGGAAAGAT GCCAAAATGT ACCCTGCTAC AACCAAAAGA GGTGCTCGTA 
TTTGATTTTG GCAGTGAAGT GTATGTCTGG CATGGAAAAG AAGTTACTTT AGCACAAAGA 
AAAGTGGCAT TCCAGCTGGC AAAACACTTA TGGAATGGCA CCTTCGACTA CTCAAATTGT 
GACATAAATC CTCTGGACCC AGGAGAATGC AATCCCCTCA TACCCAGAAA GGGACAAGGA 
CGTCCAGACT GGGCTGTGTT TGGCAGGCTC ACAGAACATA ATGAGACCAT ACTGTTCAAA 
GAAAAATTCC TTGATTGGAC AGAGCTGAAG AAACTCAATG AGAAGAATTC TAGTGAATCC 
CTTCACCAGA AGGAAGAATC CAAATCTGAT TCTAAACCAT ATGATGTCAT GCTAATGGTA 
CCTGTGCCCC AAACTGCTGT AGGCACGGTT CTGGATGGAA TGAATATTGG CCGGGGCTAT 
GGACTTGTAG AAGGAGAAGA TGGGAGGCAG TTTGAAATTA TCACTGCATC TGTGGATGTC 
TGGCACATCC TGGAGTTTGA TTATAGTAGA TTGCCTAAAC AAAGCATAGG ACAGTTCCAT 
GAGGGAGACA CATACGTGGT GAAGTGGAAG TACATGGTGA GCACTACAGT TGGAAGCAGA 
CAAAAAGGAG AGCAACAAGT AAGAGCTGTT GGAAAAGAGA AATGTGTGTA TTTCTTTTGG 
CAAGGAAGGC ACTCCACAGT GAGTGAGAAA GGGACATCGG CACTGATGAC AGTGGAACTT 
GATGAGGAGA GAGGAGCACA GGTACAAGTG CTTCAAGGGA AAGAACTACC ATGCTTCCTG 
CAGTGCTTCC AAGGAGGGAT GATTGTGCAT GCTGGAAGAA GGGAAGAGGA AGAAGAAAAT 
GCACAAAGTG ACTGGAGACT ATATTGCGTG CGAGGAGAAG TTCCCAATGA AGGAAACTTA 
CTTGAAGTAG CATGTCACTG CAGCAGCCTG CGTTCCCGGA CATCCATGAT TGTCCTCAAT 
ATAAATAAAG CTCTTATCTA CCTGTGGCAT GGCTGCAAAG CACAATCTCA CACCAAGGAT 
GTAGGAAGAA CAGCAGCCAA TAAAATAAAA GAACAATGTC CGCTGGAAGC AGGGCTGCAC 
AGCAGCAGCA AGGTGACAAT ACACGAATGT GATGAAGGGT CAGAGCCTTT GGGATTCTGG 
GATGCATTAG GAAGGCGAGA TCGAAAGGCC TATGATTGCA TGTTGCAAGA TCCGGGAAAG 
TTTAATTTCA CCCCCCGCCT CTTCAGCCTC AGTAGTTCTT CGGGAGAATT CTCAGCCACT 
GAGTACGTTT ACCCTTCAAG AGACCCTACT GTCATCAATT CTATGCCCTT CTTGCAAGAG 
GATCTTTACA CTGCCCCACA GCCAGCACTG TTCCTTGTTG ACAATCACCA TGAAGTGTAC 
CTGTGGCAAG GCTGGTGGCC TGTGGAAAAC AAAATCACTG GATCAGCTCG AATCAGGTGG 
GCTACAGACA GGAAATGCGC CATGGAGACC GTGCTCCAGT ACTGCAAAGG AAAAAATGTA 
AAGAAGCCCC CAAAGTCCTA TCTCATTCAT GCCGGCCTAG AGCCTCTGAC CTTCACTAAT 
ATGTTCCCAA GTTGGGAACA CAGGGAAGAC ATTGCAGAGA TCACAGAAAT GGATGCTGAC 
GTTTCTAATC AGATAATCCT TGTAGAAGAT GTGCTGGCCA AGCTGTGTAA AACTGTGTAT 
CCCCTAGCTG ATCTCTTAGC CAGGCCTTTA CCTGAGGGAG TTGATCCTCT GAAGCTTGAA 
ATTTATCTTT CAGATGAGGA CTTTGAGGTT GCATTAGAGA TGACAAGAGA AGAGTACAAT 
GCGCTGCCAT CTTGGAAGCA GGTTAATCTG AAAAAGGCAA AAGGACTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282096.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137582.1 Gallus gallus supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OGa31973
Clone ID Related Accession (Same CDS sequence)	XM_015282096.1
Accession Version	XM_015282096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGAGT AAAAGTTAGA 
GAGAAACTGG TGAAAGAGGA AAGTGCACGT GGAAGCCCTG AACCTGTCAC AGACAGTGTA 
TCTCAGAGAA AATCCCAGCC AGTGTCAGCC CACTACATGC CTCTTCAGAC AGAAACGTCT 
GCCTTTGATA GGGTTCTCAA TCAGCCCGTC AGTGCAGTCC GCCAACCAAT ACATGGGTAT 
ATTCAGCCTG CTGGTGTTGC ACACACGGCT CAGCTGATGG CAACAGAAGA ACCAGAAAGC 
ATCTTCGTTG GCAGCCGCCT CCTTCCAGAC AGTGTCAGCC CCTTAACGAA ATCAGCAGCA 
GCCACTGCAC CAGAGGGTGA AAAGAAAGAG ACGCTGGCTT ATGGAGCAAA GCCTGGCGAA 
GAAGAATCCC CAGAGGGTGA GAAGGCTTCC AAAGGCAGAA GACCAATTTT GCCATCTGAG 
ATTCGCAAGC AAGGGAAGAG CCACGAAGGC CTCTTTGGAG GAGAAGAACC AGATACCAGT 
GTGGGGAGCT CCTCATCTGC AAGCAAGCCG AAGGTTGGCT CAGATGTTCA GCAGGAGCAG 
GAATGCAAAA AGAAGCAGGC GCCTGAGCAG CAGCTGAAGA CACCTGAGAA CATGGAGAGG 
AGCGAAGCTG TCATGTACAT ACAGAGCGGG TCTGTATCAA AGCCAGCCCA GGCAAAGGGC 
CCCGTTTGGA AGGTGTCAAA GCATAAAGTC CTGACTCGCT CCATGTCTGA CTATACTGTG 
CCTCCTAAGC TAGAAGCTTT TCATAGTAAG AGCATGGAAG CAAACGCAGC AAATGGTGAG 
ATTGGCATGG TTGACACAAA GGTCTCTGTT GCCCAGCTCC GTAATGTCTT TCTGGAAACT 
GCTCATGCCA ACAAGAAAAC GGACCTCGAA TCTCGGTTTG AGATGTCAAC GTCAGGCAGC 
GCTTTGTCTG CCGGTGGTGA CCGTGAAAGA GGGCCGCGAA GGCCGAGGCG CTATTTCACT 
CCTGGTGAAA ATAGAAAGAC TTCTGAGCGA TTTAAAACCC AACCTATAAC GTCAGCAGAA 
CGAAAGGAGA CGGATAGGTC TATTATGTGT GCTGAAATAC CAACTGCTGA CGATGAAGAA 
AAATTAGATG ACCGAGCCAA ACTAAGTGTA GCTGCAAAGA GACTGCTTTT TAGAGAAATG 
GAAAAATCTT TTGAAATGAA GAATATTCCT AAACCACCTA CGAGAAGCTC AGCAGTAGAG 
AGAAGAATGC GGCGTTTGCA GGACAGATCA CACACGCAGC CTATTACCAG TGAGGAAGTG 
GTCATTGCAG CTACTGAAAC TACCCCTGCT TCACGTTCTG TGAATACCCA CACAGTAGTG 
GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG AGCACTGTAC AACCTACCAG CTTGCAGGCA 
TCAGCACACC AAAAAATACT AGCTAAAGAG GAGATAAGAG CAGCCAAAGA GGCGATGGGG 
CAAGATCAAT CCGATGAACC AGATTCTTCC ACATTAAGCT TGGCAGAAAA GATGGCTTTG 
TTTAACAAAC TGTCTCAGCC AGTATCCAGG GCTATATCCA CAAGGAGTCG GGGAGATATT 
AGGCACAGAA GGATGAATAC TCGTTACCAG ACCCAGCCAG TCACTCTTGG AGAAGTTGAG 
CAGATGCAAT GGAAAAAGAG TAAACCGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGCAGAGGAT AGCAGATAAT 
CAGGAGAGTC AAATGACTAT AGAAGAAAGA AAACACCTCA TCACAGCTCG AGAAGAAGCC 
TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT GACTCCACGC AGTTCACCGT AGCTGGTCGA 
ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT GCACTAACAC CAGTAGCATC CCCTTATGGT 
AACAGGCAGA AGTCTACAAC TCCGGTCACA AAGCCCTTGG AAGAAATTGA AGCTAGGCCT 
GATATGCAAT TGGAATCAGA TATGAAGCTT GACAAGCTGG AGTCATTCTT GGGAAGGCTG 
AATAACAAAG TCGCTGGAAT GCAAGAGACA GTGTTGACAG TTACAGGAAA ATCTGTGAAA 
GAGGTGATGA AGCTGGATGA TGATGAAACA TTTTCCAAAT TTTACCGCCG TGTGGATTTC 
CCTTCCTCCT CTGTGCCTTT GGACCTTGAT GAGGACTTTG ATGCCATCTT TGACCCTTAT 
GCACCAAAGT TAATATCCTC TGTAGCAGAG CACAAACGTG CAGTGAGGCC TATGCGCAGG 
GTTCAGTCCT CCAGAAACCC CCTGAAAATG CTGGCAGCAA GAGAAGATAT TCTTCATGAG 
TACACTGAAC AAAGACTGAA TGTTGCCTTC ATGGAGTCAA AGCGAATGAA AGTAGAAAAA 
ATGTCTGCAA ACTCAAATTT CTCAGAAGTA GCACTTGCTG GTTTGGCAAG CAAGGAGAAC 
TTCAGCAACG TTAGTCTGCG GAGTGTTAAC CTAACAGAGC AAAATTCTAA CAACAGTGCT 
GTGCCATACA AGAAGTTAAT GCTGCTGCAA ATTAAAGGAA GAAGACATGT TCAAACAAGA 
TTAGTTGAAC CAAGGGCCTC ATCACTGAAC AGTGGAGACT GTTTCCTATT GTTAACTCCA 
CATCTCTGTT TCTTATGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAAGC AAAGGCTTCA 
GAACTTGCAA CTTTGATTCA GACAAAGAGG GAACTTGGCT GTAGAGCTTC TTATATACAG 
ACTATAGAAG AAGGAATAAA CACCCATACC CATGCAGCCA AAGACTTCTG GAAACTCCTT 
GGTGGCCAAG CAAATTACCA GTCTGCTGGA AGACCTGAAG AGGATGAAAT GTATGAAGCT 
GCCATAATAG AAACTAATTG CATTTACCGT CTTGTAGAGG ATAAACTCAT TCCTGAAGAT 
GACTACTGGG GAAAGATGCC AAAATGTACC CTGCTACAAC CAAAAGAGGT GCTCGTATTT 
GATTTTGGCA GTGAAGTGTA TGTCTGGCAT GGAAAAGAAG TTACTTTAGC ACAAAGAAAA 
GTGGCATTCC AGCTGGCAAA ACACTTATGG AATGGCACCT TCGACTACTC AAATTGTGAC 
ATAAATCCTC TGGACCCAGG AGAATGCAAT CCCCTCATAC CCAGAAAGGG ACAAGGACGT 
CCAGACTGGG CTGTGTTTGG CAGGCTCACA GAACATAATG AGACCATACT GTTCAAAGAA 
AAATTCCTTG ATTGGACAGA GCTGAAGAAA CTCAATGAGA AGAATTCTAG TGAATCCCTT 
CACCAGAAGG AAGAATCCAA ATCTGATTCT AAACCATATG ATGTCATGCT AATGGTACCT 
GTGCCCCAAA CTGCTGTAGG CACGGTTCTG GATGGAATGA ATATTGGCCG GGGCTATGGA 
CTTGTAGAAG GAGAAGATGG GAGGCAGTTT GAAATTATCA CTGCATCTGT GGATGTCTGG 
CACATCCTGG AGTTTGATTA TAGTAGATTG CCTAAACAAA GCATAGGACA GTTCCATGAG 
GGAGACACAT ACGTGGTGAA GTGGAAGTAC ATGGTGAGCA CTACAGTTGG AAGCAGACAA 
AAAGGAGAGC AACAAGTAAG AGCTGTTGGA AAAGAGAAAT GTGTGTATTT CTTTTGGCAA 
GGAAGGCACT CCACAGTGAG TGAGAAAGGG ACATCGGCAC TGATGACAGT GGAACTTGAT 
GAGGAGAGAG GAGCACAGGT ACAAGTGCTT CAAGGGAAAG AACTACCATG CTTCCTGCAG 
TGCTTCCAAG GAGGGATGAT TGTGCATGCT GGAAGAAGGG AAGAGGAAGA AGAAAATGCA 
CAAAGTGACT GGAGACTATA TTGCGTGCGA GGAGAAGTTC CCAATGAAGG AAACTTACTT 
GAAGTAGCAT GTCACTGCAG CAGCCTGCGT TCCCGGACAT CCATGATTGT CCTCAATATA 
AATAAAGCTC TTATCTACCT GTGGCATGGC TGCAAAGCAC AATCTCACAC CAAGGATGTA 
GGAAGAACAG CAGCCAATAA AATAAAAGAA CAATGTCCGC TGGAAGCAGG GCTGCACAGC 
AGCAGCAAGG TGACAATACA CGAATGTGAT GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT 
GCATTAGGAA GGCGAGATCG AAAGGCCTAT GATTGCATGT TGCAAGATCC GGGAAAGTTT 
AATTTCACCC CCCGCCTCTT CAGCCTCAGT AGTTCTTCGG GAGAATTCTC AGCCACTGAG 
TACGTTTACC CTTCAAGAGA CCCTACTGTC ATCAATTCTA TGCCCTTCTT GCAAGAGGAT 
CTTTACACTG CCCCACAGCC AGCACTGTTC CTTGTTGACA ATCACCATGA AGTGTACCTG 
TGGCAAGGCT GGTGGCCTGT GGAAAACAAA ATCACTGGAT CAGCTCGAAT CAGGTGGGCT 
ACAGACAGGA AATGCGCCAT GGAGACCGTG CTCCAGTACT GCAAAGGAAA AAATGTAAAG 
AAGCCCCCAA AGTCCTATCT CATTCATGCC GGCCTAGAGC CTCTGACCTT CACTAATATG 
TTCCCAAGTT GGGAACACAG GGAAGACATT GCAGAGATCA CAGAAATGGA TGCTGACGTT 
TCTAATCAGA TAATCCTTGT AGAAGATGTG CTGGCCAAGC TGTGTAAAAC TGTGTATCCC 
CTAGCTGATC TCTTAGCCAG GCCTTTACCT GAGGGAGTTG ATCCTCTGAA GCTTGAAATT 
TATCTTTCAG ATGAGGACTT TGAGGTTGCA TTAGAGATGA CAAGAGAAGA GTACAATGCG 
CTGCCATCTT GGAAGCAGGT TAATCTGAAA AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137582.1
CDS	17..6847
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282096.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282096.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGAGT AAAAGTTAGA 
GAGAAACTGG TGAAAGAGGA AAGTGCACGT GGAAGCCCTG AACCTGTCAC AGACAGTGTA 
TCTCAGAGAA AATCCCAGCC AGTGTCAGCC CACTACATGC CTCTTCAGAC AGAAACGTCT 
GCCTTTGATA GGGTTCTCAA TCAGCCCGTC AGTGCAGTCC GCCAACCAAT ACATGGGTAT 
ATTCAGCCTG CTGGTGTTGC ACACACGGCT CAGCTGATGG CAACAGAAGA ACCAGAAAGC 
ATCTTCGTTG GCAGCCGCCT CCTTCCAGAC AGTGTCAGCC CCTTAACGAA ATCAGCAGCA 
GCCACTGCAC CAGAGGGTGA AAAGAAAGAG ACGCTGGCTT ATGGAGCAAA GCCTGGCGAA 
GAAGAATCCC CAGAGGGTGA GAAGGCTTCC AAAGGCAGAA GACCAATTTT GCCATCTGAG 
ATTCGCAAGC AAGGGAAGAG CCACGAAGGC CTCTTTGGAG GAGAAGAACC AGATACCAGT 
GTGGGGAGCT CCTCATCTGC AAGCAAGCCG AAGGTTGGCT CAGATGTTCA GCAGGAGCAG 
GAATGCAAAA AGAAGCAGGC GCCTGAGCAG CAGCTGAAGA CACCTGAGAA CATGGAGAGG 
AGCGAAGCTG TCATGTACAT ACAGAGCGGG TCTGTATCAA AGCCAGCCCA GGCAAAGGGC 
CCCGTTTGGA AGGTGTCAAA GCATAAAGTC CTGACTCGCT CCATGTCTGA CTATACTGTG 
CCTCCTAAGC TAGAAGCTTT TCATAGTAAG AGCATGGAAG CAAACGCAGC AAATGGTGAG 
ATTGGCATGG TTGACACAAA GGTCTCTGTT GCCCAGCTCC GTAATGTCTT TCTGGAAACT 
GCTCATGCCA ACAAGAAAAC GGACCTCGAA TCTCGGTTTG AGATGTCAAC GTCAGGCAGC 
GCTTTGTCTG CCGGTGGTGA CCGTGAAAGA GGGCCGCGAA GGCCGAGGCG CTATTTCACT 
CCTGGTGAAA ATAGAAAGAC TTCTGAGCGA TTTAAAACCC AACCTATAAC GTCAGCAGAA 
CGAAAGGAGA CGGATAGGTC TATTATGTGT GCTGAAATAC CAACTGCTGA CGATGAAGAA 
AAATTAGATG ACCGAGCCAA ACTAAGTGTA GCTGCAAAGA GACTGCTTTT TAGAGAAATG 
GAAAAATCTT TTGAAATGAA GAATATTCCT AAACCACCTA CGAGAAGCTC AGCAGTAGAG 
AGAAGAATGC GGCGTTTGCA GGACAGATCA CACACGCAGC CTATTACCAG TGAGGAAGTG 
GTCATTGCAG CTACTGAAAC TACCCCTGCT TCACGTTCTG TGAATACCCA CACAGTAGTG 
GCAAGAGTAC CTAGTCCCAC TCTAGTTAAG AGCACTGTAC AACCTACCAG CTTGCAGGCA 
TCAGCACACC AAAAAATACT AGCTAAAGAG GAGATAAGAG CAGCCAAAGA GGCGATGGGG 
CAAGATCAAT CCGATGAACC AGATTCTTCC ACATTAAGCT TGGCAGAAAA GATGGCTTTG 
TTTAACAAAC TGTCTCAGCC AGTATCCAGG GCTATATCCA CAAGGAGTCG GGGAGATATT 
AGGCACAGAA GGATGAATAC TCGTTACCAG ACCCAGCCAG TCACTCTTGG AGAAGTTGAG 
CAGATGCAAT GGAAAAAGAG TAAACCGGTG CAAAACGAAA GTGGAAAACT AACTCCCCTG 
TCAAGTACAG TTTCAACATC AGTTTCAGCA ATGGCTTCTG CCCTTTCTGC ACTGTTTGCT 
GGAGACATGC ATGCGAAGTC ACGTAGTGAT GGAAGTGTGA GCGCTGCCAC AAAAGATGAG 
TTGAGATTCC ATGCTTCAGC AGAAAGCTCA GACTCATCAG GAAAACGCAC ACAGAAGTCA 
GAACAGTGGC AGCCCTCAAT GGAAGTGCTG GAAAGCAAAA GAGCATCAAA GAAGCATGAA 
GAAGAAGGAA AGAGGTTTCT AGCACATGAG GCTAATGAAA TCAGAAAGTA CAGCTCCTTT 
GAGGATGAAA CCACACATCC TGTTCTTGAA AAAACAGGAG ACTACCATAA AGAGGCAGCC 
TACAGCTTCC TGAGGAAGGG CAGCATGGAA CTGTTTAGTT CACAACCACT TTTTCAGCCT 
CTTGAAAGGA AGGAAATTGA TACCATCATG CAAGACCACG TGGAGCCTAC CTCTGAACTC 
ACCAGCACAC CAGCAACGAG GACACTTTCC CAGACTACAG CTGCAGCATC CTGTAGGCTA 
CAGGAGCTGT CTGAACAACT GGAGGGAAAG TTCTACAAGA ATTCCTGTGA GATGTTTTCT 
GCTGGAGAGA ACAAAATACA GACAACTGAG GATGCCACCG ATAGCTCCAG CAAAACGATG 
TCAATTAAAG AAAGGTTGGC ATTGCTGAAG AAAAGTGGTG AAGAAGATTG GAAGAGCAGG 
CTCAGTAAAA AGCAGGAATA TGCAAAAGTG TCTGCCACTG ATCGTAGCGC ACAGATGCAA 
GAAGTTGAGC AGCTGTTGAA AAAGGAACCT GTATATGCTT CTACTTACTC TCCTGTTATA 
CCTGCTGTCC ATAAATTTCA TCCTTTTCTA CCTATTAATC AGCAGAGGAT AGCAGATAAT 
CAGGAGAGTC AAATGACTAT AGAAGAAAGA AAACACCTCA TCACAGCTCG AGAAGAAGCC 
TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT GACTCCACGC AGTTCACCGT AGCTGGTCGA 
ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT GCACTAACAC CAGTAGCATC CCCTTATGGT 
AACAGGCAGA AGTCTACAAC TCCGGTCACA AAGCCCTTGG AAGAAATTGA AGCTAGGCCT 
GATATGCAAT TGGAATCAGA TATGAAGCTT GACAAGCTGG AGTCATTCTT GGGAAGGCTG 
AATAACAAAG TCGCTGGAAT GCAAGAGACA GTGTTGACAG TTACAGGAAA ATCTGTGAAA 
GAGGTGATGA AGCTGGATGA TGATGAAACA TTTTCCAAAT TTTACCGCCG TGTGGATTTC 
CCTTCCTCCT CTGTGCCTTT GGACCTTGAT GAGGACTTTG ATGCCATCTT TGACCCTTAT 
GCACCAAAGT TAATATCCTC TGTAGCAGAG CACAAACGTG CAGTGAGGCC TATGCGCAGG 
GTTCAGTCCT CCAGAAACCC CCTGAAAATG CTGGCAGCAA GAGAAGATAT TCTTCATGAG 
TACACTGAAC AAAGACTGAA TGTTGCCTTC ATGGAGTCAA AGCGAATGAA AGTAGAAAAA 
ATGTCTGCAA ACTCAAATTT CTCAGAAGTA GCACTTGCTG GTTTGGCAAG CAAGGAGAAC 
TTCAGCAACG TTAGTCTGCG GAGTGTTAAC CTAACAGAGC AAAATTCTAA CAACAGTGCT 
GTGCCATACA AGAAGTTAAT GCTGCTGCAA ATTAAAGGAA GAAGACATGT TCAAACAAGA 
TTAGTTGAAC CAAGGGCCTC ATCACTGAAC AGTGGAGACT GTTTCCTATT GTTAACTCCA 
CATCTCTGTT TCTTATGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAAGC AAAGGCTTCA 
GAACTTGCAA CTTTGATTCA GACAAAGAGG GAACTTGGCT GTAGAGCTTC TTATATACAG 
ACTATAGAAG AAGGAATAAA CACCCATACC CATGCAGCCA AAGACTTCTG GAAACTCCTT 
GGTGGCCAAG CAAATTACCA GTCTGCTGGA AGACCTGAAG AGGATGAAAT GTATGAAGCT 
GCCATAATAG AAACTAATTG CATTTACCGT CTTGTAGAGG ATAAACTCAT TCCTGAAGAT 
GACTACTGGG GAAAGATGCC AAAATGTACC CTGCTACAAC CAAAAGAGGT GCTCGTATTT 
GATTTTGGCA GTGAAGTGTA TGTCTGGCAT GGAAAAGAAG TTACTTTAGC ACAAAGAAAA 
GTGGCATTCC AGCTGGCAAA ACACTTATGG AATGGCACCT TCGACTACTC AAATTGTGAC 
ATAAATCCTC TGGACCCAGG AGAATGCAAT CCCCTCATAC CCAGAAAGGG ACAAGGACGT 
CCAGACTGGG CTGTGTTTGG CAGGCTCACA GAACATAATG AGACCATACT GTTCAAAGAA 
AAATTCCTTG ATTGGACAGA GCTGAAGAAA CTCAATGAGA AGAATTCTAG TGAATCCCTT 
CACCAGAAGG AAGAATCCAA ATCTGATTCT AAACCATATG ATGTCATGCT AATGGTACCT 
GTGCCCCAAA CTGCTGTAGG CACGGTTCTG GATGGAATGA ATATTGGCCG GGGCTATGGA 
CTTGTAGAAG GAGAAGATGG GAGGCAGTTT GAAATTATCA CTGCATCTGT GGATGTCTGG 
CACATCCTGG AGTTTGATTA TAGTAGATTG CCTAAACAAA GCATAGGACA GTTCCATGAG 
GGAGACACAT ACGTGGTGAA GTGGAAGTAC ATGGTGAGCA CTACAGTTGG AAGCAGACAA 
AAAGGAGAGC AACAAGTAAG AGCTGTTGGA AAAGAGAAAT GTGTGTATTT CTTTTGGCAA 
GGAAGGCACT CCACAGTGAG TGAGAAAGGG ACATCGGCAC TGATGACAGT GGAACTTGAT 
GAGGAGAGAG GAGCACAGGT ACAAGTGCTT CAAGGGAAAG AACTACCATG CTTCCTGCAG 
TGCTTCCAAG GAGGGATGAT TGTGCATGCT GGAAGAAGGG AAGAGGAAGA AGAAAATGCA 
CAAAGTGACT GGAGACTATA TTGCGTGCGA GGAGAAGTTC CCAATGAAGG AAACTTACTT 
GAAGTAGCAT GTCACTGCAG CAGCCTGCGT TCCCGGACAT CCATGATTGT CCTCAATATA 
AATAAAGCTC TTATCTACCT GTGGCATGGC TGCAAAGCAC AATCTCACAC CAAGGATGTA 
GGAAGAACAG CAGCCAATAA AATAAAAGAA CAATGTCCGC TGGAAGCAGG GCTGCACAGC 
AGCAGCAAGG TGACAATACA CGAATGTGAT GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT 
GCATTAGGAA GGCGAGATCG AAAGGCCTAT GATTGCATGT TGCAAGATCC GGGAAAGTTT 
AATTTCACCC CCCGCCTCTT CAGCCTCAGT AGTTCTTCGG GAGAATTCTC AGCCACTGAG 
TACGTTTACC CTTCAAGAGA CCCTACTGTC ATCAATTCTA TGCCCTTCTT GCAAGAGGAT 
CTTTACACTG CCCCACAGCC AGCACTGTTC CTTGTTGACA ATCACCATGA AGTGTACCTG 
TGGCAAGGCT GGTGGCCTGT GGAAAACAAA ATCACTGGAT CAGCTCGAAT CAGGTGGGCT 
ACAGACAGGA AATGCGCCAT GGAGACCGTG CTCCAGTACT GCAAAGGAAA AAATGTAAAG 
AAGCCCCCAA AGTCCTATCT CATTCATGCC GGCCTAGAGC CTCTGACCTT CACTAATATG 
TTCCCAAGTT GGGAACACAG GGAAGACATT GCAGAGATCA CAGAAATGGA TGCTGACGTT 
TCTAATCAGA TAATCCTTGT AGAAGATGTG CTGGCCAAGC TGTGTAAAAC TGTGTATCCC 
CTAGCTGATC TCTTAGCCAG GCCTTTACCT GAGGGAGTTG ATCCTCTGAA GCTTGAAATT 
TATCTTTCAG ATGAGGACTT TGAGGTTGCA TTAGAGATGA CAAGAGAAGA GTACAATGCG 
CTGCCATCTT GGAAGCAGGT TAATCTGAAA AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282097.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137583.1 Gallus gallus supervillin (SVIL), transcript variant X9, mRNA.

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CloneID	OGa31974
Clone ID Related Accession (Same CDS sequence)	XM_015282097.1
Accession Version	XM_015282097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACCT TGCAGGCATC AGCACACCAA 
AAAATACTAG CTAAAGAGGA GATAAGAGCA GCCAAAGAGG CGATGGGGCA AGATCAATCC 
GATGAACCAG ATTCTTCCAC ATTAAGCTTG GCAGAAAAGA TGGCTTTGTT TAACAAACTG 
TCTCAGCCAG TATCCAGGGC TATATCCACA AGGAGTCGGG GAGATATTAG GCACAGAAGG 
ATGAATACTC GTTACCAGAC CCAGCCAGTC ACTCTTGGAG AAGTTGAGCA GATGCAATGG 
AAAAAGAGTA AACCGGTGCA AAACGAAAGT GGAAAACTAA CTCCCCTGTC AAGTACAGTT 
TCAACATCAG TTTCAGCAAT GGCTTCTGCC CTTTCTGCAC TGTTTGCTGG AGACATGCAT 
GCGAAGTCAC GTAGTGATGG AAGTGTGAGC GCTGCCACAA AAGATGAGTT GAGATTCCAT 
GCTTCAGCAG AAAGCTCAGA CTCATCAGGA AAACGCACAC AGAAGTCAGA ACAGTGGCAG 
CCCTCAATGG AAGTGCTGGA AAGCAAAAGA GCATCAAAGA AGCATGAAGA AGAAGGAAAG 
AGGTTTCTAG CACATGAGGC TAATGAAATC AGAAAGTACA GCTCCTTTGA GGATGAAACC 
ACACATCCTG TTCTTGAAAA AACAGGAGAC TACCATAAAG AGGCAGCCTA CAGCTTCCTG 
AGGAAGGGCA GCATGGAACT GTTTAGTTCA CAACCACTTT TTCAGCCTCT TGAAAGGAAG 
GAAATTGATA CCATCATGCA AGACCACGTG GAGCCTACCT CTGAACTCAC CAGCACACCA 
GCAACGAGGA CACTTTCCCA GACTACAGCT GCAGCATCCT GTAGGCTACA GGAGCTGTCT 
GAACAACTGG AGGGAAAGTT CTACAAGAAT TCCTGTGAGA TGTTTTCTGC TGGAGAGAAC 
AAAATACAGA CAACTGAGGA TGCCACCGAT AGCTCCAGCA AAACGATGTC AATTAAAGAA 
AGGTTGGCAT TGCTGAAGAA AAGTGGTGAA GAAGATTGGA AGAGCAGGCT CAGTAAAAAG 
CAGGAATATG CAAAAGTGTC TGCCACTGAT CGTAGCGCAC AGATGCAAGA AGTTGAGCAG 
CTGTTGAAAA AGGAACCTGT ATATGCTTCT ACTTACTCTC CTGTTATACC TGCTGTCCAT 
AAATTTCATC CTTTTCTACC TATTAATCAG CAGAGGATAG CAGATAATCA GGAGAGTCAA 
ATGACTATAG AAGAAAGAAA ACACCTCATC ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA 
GGTAAAGGCG CAGCAAATGA CTCCACGCAG TTCACCGTAG CTGGTCGAAT GGTAAAAAAA 
GGCCTGGCTT CTCCAAGTGC ACTAACACCA GTAGCATCCC CTTATGGTAA CAGGCAGAAG 
TCTACAACTC CGGTCACAAA GCCCTTGGAA GAAATTGAAG CTAGGCCTGA TATGCAATTG 
GAATCAGATA TGAAGCTTGA CAAGCTGGAG TCATTCTTGG GAAGGCTGAA TAACAAAGTC 
GCTGGAATGC AAGAGACAGT GTTGACAGTT ACAGGAAAAT CTGTGAAAGA GGTGATGAAG 
CTGGATGATG ATGAAACATT TTCCAAATTT TACCGCCGTG TGGATTTCCC TTCCTCCTCT 
GTGCCTTTGG ACCTTGATGA GGACTTTGAT GCCATCTTTG ACCCTTATGC ACCAAAGTTA 
ATATCCTCTG TAGCAGAGCA CAAACGTGCA GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC 
AGAAACCCCC TGAAAATGCT GGCAGCAAGA GAAGATATTC TTCATGAGTA CACTGAACAA 
AGACTGAATG TTGCCTTCAT GGAGTCAAAG CGAATGAAAG TAGAAAAAAT GTCTGCAAAC 
TCAAATTTCT CAGAAGTAGC ACTTGCTGGT TTGGCAAGCA AGGAGAACTT CAGCAACGTT 
AGTCTGCGGA GTGTTAACCT AACAGAGCAA AATTCTAACA ACAGTGCTGT GCCATACAAG 
AAGTTAATGC TGCTGCAAAT TAAAGGAAGA AGACATGTTC AAACAAGATT AGTTGAACCA 
AGGGCCTCAT CACTGAACAG TGGAGACTGT TTCCTATTGT TAACTCCACA TCTCTGTTTC 
TTATGGGTAG GAGAGTTTGC AAATGTCATA GAAAAAGCAA AGGCTTCAGA ACTTGCAACT 
TTGATTCAGA CAAAGAGGGA ACTTGGCTGT AGAGCTTCTT ATATACAGAC TATAGAAGAA 
GGAATAAACA CCCATACCCA TGCAGCCAAA GACTTCTGGA AACTCCTTGG TGGCCAAGCA 
AATTACCAGT CTGCTGGAAG ACCTGAAGAG GATGAAATGT ATGAAGCTGC CATAATAGAA 
ACTAATTGCA TTTACCGTCT TGTAGAGGAT AAACTCATTC CTGAAGATGA CTACTGGGGA 
AAGATGCCAA AATGTACCCT GCTACAACCA AAAGAGGTGC TCGTATTTGA TTTTGGCAGT 
GAAGTGTATG TCTGGCATGG AAAAGAAGTT ACTTTAGCAC AAAGAAAAGT GGCATTCCAG 
CTGGCAAAAC ACTTATGGAA TGGCACCTTC GACTACTCAA ATTGTGACAT AAATCCTCTG 
GACCCAGGAG AATGCAATCC CCTCATACCC AGAAAGGGAC AAGGACGTCC AGACTGGGCT 
GTGTTTGGCA GGCTCACAGA ACATAATGAG ACCATACTGT TCAAAGAAAA ATTCCTTGAT 
TGGACAGAGC TGAAGAAACT CAATGAGAAG AATTCTAGTG AATCCCTTCA CCAGAAGGAA 
GAATCCAAAT CTGATTCTAA ACCATATGAT GTCATGCTAA TGGTACCTGT GCCCCAAACT 
GCTGTAGGCA CGGTTCTGGA TGGAATGAAT ATTGGCCGGG GCTATGGACT TGTAGAAGGA 
GAAGATGGGA GGCAGTTTGA AATTATCACT GCATCTGTGG ATGTCTGGCA CATCCTGGAG 
TTTGATTATA GTAGATTGCC TAAACAAAGC ATAGGACAGT TCCATGAGGG AGACACATAC 
GTGGTGAAGT GGAAGTACAT GGTGAGCACT ACAGTTGGAA GCAGACAAAA AGGAGAGCAA 
CAAGTAAGAG CTGTTGGAAA AGAGAAATGT GTGTATTTCT TTTGGCAAGG AAGGCACTCC 
ACAGTGAGTG AGAAAGGGAC ATCGGCACTG ATGACAGTGG AACTTGATGA GGAGAGAGGA 
GCACAGGTAC AAGTGCTTCA AGGGAAAGAA CTACCATGCT TCCTGCAGTG CTTCCAAGGA 
GGGATGATTG TGCATGCTGG AAGAAGGGAA GAGGAAGAAG AAAATGCACA AAGTGACTGG 
AGACTATATT GCGTGCGAGG AGAAGTTCCC AATGAAGGAA ACTTACTTGA AGTAGCATGT 
CACTGCAGCA GCCTGCGTTC CCGGACATCC ATGATTGTCC TCAATATAAA TAAAGCTCTT 
ATCTACCTGT GGCATGGCTG CAAAGCACAA TCTCACACCA AGGATGTAGG AAGAACAGCA 
GCCAATAAAA TAAAAGAACA ATGTCCGCTG GAAGCAGGGC TGCACAGCAG CAGCAAGGTG 
ACAATACACG AATGTGATGA AGGGTCAGAG CCTTTGGGAT TCTGGGATGC ATTAGGAAGG 
CGAGATCGAA AGGCCTATGA TTGCATGTTG CAAGATCCGG GAAAGTTTAA TTTCACCCCC 
CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA GAATTCTCAG CCACTGAGTA CGTTTACCCT 
TCAAGAGACC CTACTGTCAT CAATTCTATG CCCTTCTTGC AAGAGGATCT TTACACTGCC 
CCACAGCCAG CACTGTTCCT TGTTGACAAT CACCATGAAG TGTACCTGTG GCAAGGCTGG 
TGGCCTGTGG AAAACAAAAT CACTGGATCA GCTCGAATCA GGTGGGCTAC AGACAGGAAA 
TGCGCCATGG AGACCGTGCT CCAGTACTGC AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG 
TCCTATCTCA TTCATGCCGG CCTAGAGCCT CTGACCTTCA CTAATATGTT CCCAAGTTGG 
GAACACAGGG AAGACATTGC AGAGATCACA GAAATGGATG CTGACGTTTC TAATCAGATA 
ATCCTTGTAG AAGATGTGCT GGCCAAGCTG TGTAAAACTG TGTATCCCCT AGCTGATCTC 
TTAGCCAGGC CTTTACCTGA GGGAGTTGAT CCTCTGAAGC TTGAAATTTA TCTTTCAGAT 
GAGGACTTTG AGGTTGCATT AGAGATGACA AGAGAAGAGT ACAATGCGCT GCCATCTTGG 
AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137583.1
CDS	17..6835
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282097.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282097.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACCT TGCAGGCATC AGCACACCAA 
AAAATACTAG CTAAAGAGGA GATAAGAGCA GCCAAAGAGG CGATGGGGCA AGATCAATCC 
GATGAACCAG ATTCTTCCAC ATTAAGCTTG GCAGAAAAGA TGGCTTTGTT TAACAAACTG 
TCTCAGCCAG TATCCAGGGC TATATCCACA AGGAGTCGGG GAGATATTAG GCACAGAAGG 
ATGAATACTC GTTACCAGAC CCAGCCAGTC ACTCTTGGAG AAGTTGAGCA GATGCAATGG 
AAAAAGAGTA AACCGGTGCA AAACGAAAGT GGAAAACTAA CTCCCCTGTC AAGTACAGTT 
TCAACATCAG TTTCAGCAAT GGCTTCTGCC CTTTCTGCAC TGTTTGCTGG AGACATGCAT 
GCGAAGTCAC GTAGTGATGG AAGTGTGAGC GCTGCCACAA AAGATGAGTT GAGATTCCAT 
GCTTCAGCAG AAAGCTCAGA CTCATCAGGA AAACGCACAC AGAAGTCAGA ACAGTGGCAG 
CCCTCAATGG AAGTGCTGGA AAGCAAAAGA GCATCAAAGA AGCATGAAGA AGAAGGAAAG 
AGGTTTCTAG CACATGAGGC TAATGAAATC AGAAAGTACA GCTCCTTTGA GGATGAAACC 
ACACATCCTG TTCTTGAAAA AACAGGAGAC TACCATAAAG AGGCAGCCTA CAGCTTCCTG 
AGGAAGGGCA GCATGGAACT GTTTAGTTCA CAACCACTTT TTCAGCCTCT TGAAAGGAAG 
GAAATTGATA CCATCATGCA AGACCACGTG GAGCCTACCT CTGAACTCAC CAGCACACCA 
GCAACGAGGA CACTTTCCCA GACTACAGCT GCAGCATCCT GTAGGCTACA GGAGCTGTCT 
GAACAACTGG AGGGAAAGTT CTACAAGAAT TCCTGTGAGA TGTTTTCTGC TGGAGAGAAC 
AAAATACAGA CAACTGAGGA TGCCACCGAT AGCTCCAGCA AAACGATGTC AATTAAAGAA 
AGGTTGGCAT TGCTGAAGAA AAGTGGTGAA GAAGATTGGA AGAGCAGGCT CAGTAAAAAG 
CAGGAATATG CAAAAGTGTC TGCCACTGAT CGTAGCGCAC AGATGCAAGA AGTTGAGCAG 
CTGTTGAAAA AGGAACCTGT ATATGCTTCT ACTTACTCTC CTGTTATACC TGCTGTCCAT 
AAATTTCATC CTTTTCTACC TATTAATCAG CAGAGGATAG CAGATAATCA GGAGAGTCAA 
ATGACTATAG AAGAAAGAAA ACACCTCATC ACAGCTCGAG AAGAAGCCTG GAAGTCCAGA 
GGTAAAGGCG CAGCAAATGA CTCCACGCAG TTCACCGTAG CTGGTCGAAT GGTAAAAAAA 
GGCCTGGCTT CTCCAAGTGC ACTAACACCA GTAGCATCCC CTTATGGTAA CAGGCAGAAG 
TCTACAACTC CGGTCACAAA GCCCTTGGAA GAAATTGAAG CTAGGCCTGA TATGCAATTG 
GAATCAGATA TGAAGCTTGA CAAGCTGGAG TCATTCTTGG GAAGGCTGAA TAACAAAGTC 
GCTGGAATGC AAGAGACAGT GTTGACAGTT ACAGGAAAAT CTGTGAAAGA GGTGATGAAG 
CTGGATGATG ATGAAACATT TTCCAAATTT TACCGCCGTG TGGATTTCCC TTCCTCCTCT 
GTGCCTTTGG ACCTTGATGA GGACTTTGAT GCCATCTTTG ACCCTTATGC ACCAAAGTTA 
ATATCCTCTG TAGCAGAGCA CAAACGTGCA GTGAGGCCTA TGCGCAGGGT TCAGTCCTCC 
AGAAACCCCC TGAAAATGCT GGCAGCAAGA GAAGATATTC TTCATGAGTA CACTGAACAA 
AGACTGAATG TTGCCTTCAT GGAGTCAAAG CGAATGAAAG TAGAAAAAAT GTCTGCAAAC 
TCAAATTTCT CAGAAGTAGC ACTTGCTGGT TTGGCAAGCA AGGAGAACTT CAGCAACGTT 
AGTCTGCGGA GTGTTAACCT AACAGAGCAA AATTCTAACA ACAGTGCTGT GCCATACAAG 
AAGTTAATGC TGCTGCAAAT TAAAGGAAGA AGACATGTTC AAACAAGATT AGTTGAACCA 
AGGGCCTCAT CACTGAACAG TGGAGACTGT TTCCTATTGT TAACTCCACA TCTCTGTTTC 
TTATGGGTAG GAGAGTTTGC AAATGTCATA GAAAAAGCAA AGGCTTCAGA ACTTGCAACT 
TTGATTCAGA CAAAGAGGGA ACTTGGCTGT AGAGCTTCTT ATATACAGAC TATAGAAGAA 
GGAATAAACA CCCATACCCA TGCAGCCAAA GACTTCTGGA AACTCCTTGG TGGCCAAGCA 
AATTACCAGT CTGCTGGAAG ACCTGAAGAG GATGAAATGT ATGAAGCTGC CATAATAGAA 
ACTAATTGCA TTTACCGTCT TGTAGAGGAT AAACTCATTC CTGAAGATGA CTACTGGGGA 
AAGATGCCAA AATGTACCCT GCTACAACCA AAAGAGGTGC TCGTATTTGA TTTTGGCAGT 
GAAGTGTATG TCTGGCATGG AAAAGAAGTT ACTTTAGCAC AAAGAAAAGT GGCATTCCAG 
CTGGCAAAAC ACTTATGGAA TGGCACCTTC GACTACTCAA ATTGTGACAT AAATCCTCTG 
GACCCAGGAG AATGCAATCC CCTCATACCC AGAAAGGGAC AAGGACGTCC AGACTGGGCT 
GTGTTTGGCA GGCTCACAGA ACATAATGAG ACCATACTGT TCAAAGAAAA ATTCCTTGAT 
TGGACAGAGC TGAAGAAACT CAATGAGAAG AATTCTAGTG AATCCCTTCA CCAGAAGGAA 
GAATCCAAAT CTGATTCTAA ACCATATGAT GTCATGCTAA TGGTACCTGT GCCCCAAACT 
GCTGTAGGCA CGGTTCTGGA TGGAATGAAT ATTGGCCGGG GCTATGGACT TGTAGAAGGA 
GAAGATGGGA GGCAGTTTGA AATTATCACT GCATCTGTGG ATGTCTGGCA CATCCTGGAG 
TTTGATTATA GTAGATTGCC TAAACAAAGC ATAGGACAGT TCCATGAGGG AGACACATAC 
GTGGTGAAGT GGAAGTACAT GGTGAGCACT ACAGTTGGAA GCAGACAAAA AGGAGAGCAA 
CAAGTAAGAG CTGTTGGAAA AGAGAAATGT GTGTATTTCT TTTGGCAAGG AAGGCACTCC 
ACAGTGAGTG AGAAAGGGAC ATCGGCACTG ATGACAGTGG AACTTGATGA GGAGAGAGGA 
GCACAGGTAC AAGTGCTTCA AGGGAAAGAA CTACCATGCT TCCTGCAGTG CTTCCAAGGA 
GGGATGATTG TGCATGCTGG AAGAAGGGAA GAGGAAGAAG AAAATGCACA AAGTGACTGG 
AGACTATATT GCGTGCGAGG AGAAGTTCCC AATGAAGGAA ACTTACTTGA AGTAGCATGT 
CACTGCAGCA GCCTGCGTTC CCGGACATCC ATGATTGTCC TCAATATAAA TAAAGCTCTT 
ATCTACCTGT GGCATGGCTG CAAAGCACAA TCTCACACCA AGGATGTAGG AAGAACAGCA 
GCCAATAAAA TAAAAGAACA ATGTCCGCTG GAAGCAGGGC TGCACAGCAG CAGCAAGGTG 
ACAATACACG AATGTGATGA AGGGTCAGAG CCTTTGGGAT TCTGGGATGC ATTAGGAAGG 
CGAGATCGAA AGGCCTATGA TTGCATGTTG CAAGATCCGG GAAAGTTTAA TTTCACCCCC 
CGCCTCTTCA GCCTCAGTAG TTCTTCGGGA GAATTCTCAG CCACTGAGTA CGTTTACCCT 
TCAAGAGACC CTACTGTCAT CAATTCTATG CCCTTCTTGC AAGAGGATCT TTACACTGCC 
CCACAGCCAG CACTGTTCCT TGTTGACAAT CACCATGAAG TGTACCTGTG GCAAGGCTGG 
TGGCCTGTGG AAAACAAAAT CACTGGATCA GCTCGAATCA GGTGGGCTAC AGACAGGAAA 
TGCGCCATGG AGACCGTGCT CCAGTACTGC AAAGGAAAAA ATGTAAAGAA GCCCCCAAAG 
TCCTATCTCA TTCATGCCGG CCTAGAGCCT CTGACCTTCA CTAATATGTT CCCAAGTTGG 
GAACACAGGG AAGACATTGC AGAGATCACA GAAATGGATG CTGACGTTTC TAATCAGATA 
ATCCTTGTAG AAGATGTGCT GGCCAAGCTG TGTAAAACTG TGTATCCCCT AGCTGATCTC 
TTAGCCAGGC CTTTACCTGA GGGAGTTGAT CCTCTGAAGC TTGAAATTTA TCTTTCAGAT 
GAGGACTTTG AGGTTGCATT AGAGATGACA AGAGAAGAGT ACAATGCGCT GCCATCTTGG 
AAGCAGGTTA ATCTGAAAAA GGCAAAAGGA CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282098.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137584.1 Gallus gallus supervillin (SVIL), transcript variant X10, mRNA.

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CloneID	OGa31975
Clone ID Related Accession (Same CDS sequence)	XM_015282098.1
Accession Version	XM_015282098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACCT TGCAGGCATC AGCACACCAA 
AAAATACTAG CTAAAGAGGA GATAAGAGCA GCCAAAGAGG CGATGGGGCA AGATCAATCC 
GATGAACCAG ATTCTTCCAC ATTAAGCTTG GCAGAAAAGA TGGCTTTGTT TAACAAACTG 
TCTCAGCCAG TATCCAGGGC TATATCCACA AGGAGTCGGG GAGATATTAG GCACAGAAGG 
ATGAATACTC GTTACCAGAC CCAGCCAGTC ACTCTTGGAG AAGTTGAGCA GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137584.1
CDS	17..6811
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282098.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282098.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACCT TGCAGGCATC AGCACACCAA 
AAAATACTAG CTAAAGAGGA GATAAGAGCA GCCAAAGAGG CGATGGGGCA AGATCAATCC 
GATGAACCAG ATTCTTCCAC ATTAAGCTTG GCAGAAAAGA TGGCTTTGTT TAACAAACTG 
TCTCAGCCAG TATCCAGGGC TATATCCACA AGGAGTCGGG GAGATATTAG GCACAGAAGG 
ATGAATACTC GTTACCAGAC CCAGCCAGTC ACTCTTGGAG AAGTTGAGCA GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282099.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137585.1 Gallus gallus supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OGa31976
Clone ID Related Accession (Same CDS sequence)	XM_015282099.1
Accession Version	XM_015282099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CTGAAACTAC CCCTGCTTCA 
CGTTCTGTGA ATACCCACAC AGTAGTGGCA AGAGTACCTA GTCCCACTCT AGTTAAGAGC 
ACTGTACAAC CTACCAGCTT GCAGGCATCA GCACACCAAA AAATACTAGC TAAAGAGGAG 
ATAAGAGCAG CCAAAGAGGC GATGGGGCAA GATCAATCCG ATGAACCAGA TTCTTCCACA 
TTAAGCTTGG CAGAAAAGAT GGCTTTGTTT AACAAACTGT CTCAGCCAGT ATCCAGGGCT 
ATATCCACAA GGAGTCGGGG AGATATTAGG CACAGAAGGA TGAATACTCG TTACCAGACC 
CAGCCAGTCA CTCTTGGAGA AGTTGAGCAG ATGCAATGGA AAAAGAGTAA ACCGGTGCAA 
AACGAAAGTG GAAAACTAAC TCCCCTGTCA AGTACAGTTT CAACATCAGT TTCAGCAATG 
GCTTCTGCCC TTTCTGCACT GTTTGCTGGA GACATGCATG CGAAGTCACG TAGTGATGGA 
AGTGTGAGCG CTGCCACAAA AGATGAGTTG AGATTCCATG CTTCAGCAGA AAGCTCAGAC 
TCATCAGGAA AACGCACACA GAAGTCAGAA CAGTGGCAGC CCTCAATGGA AGTGCTGGAA 
AGCAAAAGAG CATCAAAGAA GCATGAAGAA GAAGGAAAGA GGTTTCTAGC ACATGAGGCT 
AATGAAATCA GAAAGTACAG CTCCTTTGAG GATGAAACCA CACATCCTGT TCTTGAAAAA 
ACAGGAGACT ACCATAAAGA GGCAGCCTAC AGCTTCCTGA GGAAGGGCAG CATGGAACTG 
TTTAGTTCAC AACCACTTTT TCAGCCTCTT GAAAGGAAGG AAATTGATAC CATCATGCAA 
GACCACGTGG AGCCTACCTC TGAACTCACC AGCACACCAG CAACGAGGAC ACTTTCCCAG 
ACTACAGCTG CAGCATCCTG TAGGCTACAG GAGCTGTCTG AACAACTGGA GGGAAAGTTC 
TACAAGAATT CCTGTGAGAT GTTTTCTGCT GGAGAGAACA AAATACAGAC AACTGAGGAT 
GCCACCGATA GCTCCAGCAA AACGATGTCA ATTAAAGAAA GGTTGGCATT GCTGAAGAAA 
AGTGGTGAAG AAGATTGGAA GAGCAGGCTC AGTAAAAAGC AGGAATATGC AAAAGTGTCT 
GCCACTGATC GTAGCGCACA GATGCAAGAA GTTGAGCAGC TGTTGAAAAA GGAACCTGTA 
TATGCTTCTA CTTACTCTCC TGTTATACCT GCTGTCCATA AATTTCATCC TTTTCTACCT 
ATTAATCAGC AGAGGATAGC AGATAATCAG GAGAGTCAAA TGACTATAGA AGAAAGAAAA 
CACCTCATCA CAGCTCGAGA AGAAGCCTGG AAGTCCAGAG GTAAAGGCGC AGCAAATGAC 
TCCACGCAGT TCACCGTAGC TGGTCGAATG GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA 
CTAACACCAG TAGCATCCCC TTATGGTAAC AGGCAGAAGT CTACAACTCC GGTCACAAAG 
CCCTTGGAAG AAATTGAAGC TAGGCCTGAT ATGCAATTGG AATCAGATAT GAAGCTTGAC 
AAGCTGGAGT CATTCTTGGG AAGGCTGAAT AACAAAGTCG CTGGAATGCA AGAGACAGTG 
TTGACAGTTA CAGGAAAATC TGTGAAAGAG GTGATGAAGC TGGATGATGA TGAAACATTT 
TCCAAATTTT ACCGCCGTGT GGATTTCCCT TCCTCCTCTG TGCCTTTGGA CCTTGATGAG 
GACTTTGATG CCATCTTTGA CCCTTATGCA CCAAAGTTAA TATCCTCTGT AGCAGAGCAC 
AAACGTGCAG TGAGGCCTAT GCGCAGGGTT CAGTCCTCCA GAAACCCCCT GAAAATGCTG 
GCAGCAAGAG AAGATATTCT TCATGAGTAC ACTGAACAAA GACTGAATGT TGCCTTCATG 
GAGTCAAAGC GAATGAAAGT AGAAAAAATG TCTGCAAACT CAAATTTCTC AGAAGTAGCA 
CTTGCTGGTT TGGCAAGCAA GGAGAACTTC AGCAACGTTA GTCTGCGGAG TGTTAACCTA 
ACAGAGCAAA ATTCTAACAA CAGTGCTGTG CCATACAAGA AGTTAATGCT GCTGCAAATT 
AAAGGAAGAA GACATGTTCA AACAAGATTA GTTGAACCAA GGGCCTCATC ACTGAACAGT 
GGAGACTGTT TCCTATTGTT AACTCCACAT CTCTGTTTCT TATGGGTAGG AGAGTTTGCA 
AATGTCATAG AAAAAGCAAA GGCTTCAGAA CTTGCAACTT TGATTCAGAC AAAGAGGGAA 
CTTGGCTGTA GAGCTTCTTA TATACAGACT ATAGAAGAAG GAATAAACAC CCATACCCAT 
GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT GGCCAAGCAA ATTACCAGTC TGCTGGAAGA 
CCTGAAGAGG ATGAAATGTA TGAAGCTGCC ATAATAGAAA CTAATTGCAT TTACCGTCTT 
GTAGAGGATA AACTCATTCC TGAAGATGAC TACTGGGGAA AGATGCCAAA ATGTACCCTG 
CTACAACCAA AAGAGGTGCT CGTATTTGAT TTTGGCAGTG AAGTGTATGT CTGGCATGGA 
AAAGAAGTTA CTTTAGCACA AAGAAAAGTG GCATTCCAGC TGGCAAAACA CTTATGGAAT 
GGCACCTTCG ACTACTCAAA TTGTGACATA AATCCTCTGG ACCCAGGAGA ATGCAATCCC 
CTCATACCCA GAAAGGGACA AGGACGTCCA GACTGGGCTG TGTTTGGCAG GCTCACAGAA 
CATAATGAGA CCATACTGTT CAAAGAAAAA TTCCTTGATT GGACAGAGCT GAAGAAACTC 
AATGAGAAGA ATTCTAGTGA ATCCCTTCAC CAGAAGGAAG AATCCAAATC TGATTCTAAA 
CCATATGATG TCATGCTAAT GGTACCTGTG CCCCAAACTG CTGTAGGCAC GGTTCTGGAT 
GGAATGAATA TTGGCCGGGG CTATGGACTT GTAGAAGGAG AAGATGGGAG GCAGTTTGAA 
ATTATCACTG CATCTGTGGA TGTCTGGCAC ATCCTGGAGT TTGATTATAG TAGATTGCCT 
AAACAAAGCA TAGGACAGTT CCATGAGGGA GACACATACG TGGTGAAGTG GAAGTACATG 
GTGAGCACTA CAGTTGGAAG CAGACAAAAA GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA 
GAGAAATGTG TGTATTTCTT TTGGCAAGGA AGGCACTCCA CAGTGAGTGA GAAAGGGACA 
TCGGCACTGA TGACAGTGGA ACTTGATGAG GAGAGAGGAG CACAGGTACA AGTGCTTCAA 
GGGAAAGAAC TACCATGCTT CCTGCAGTGC TTCCAAGGAG GGATGATTGT GCATGCTGGA 
AGAAGGGAAG AGGAAGAAGA AAATGCACAA AGTGACTGGA GACTATATTG CGTGCGAGGA 
GAAGTTCCCA ATGAAGGAAA CTTACTTGAA GTAGCATGTC ACTGCAGCAG CCTGCGTTCC 
CGGACATCCA TGATTGTCCT CAATATAAAT AAAGCTCTTA TCTACCTGTG GCATGGCTGC 
AAAGCACAAT CTCACACCAA GGATGTAGGA AGAACAGCAG CCAATAAAAT AAAAGAACAA 
TGTCCGCTGG AAGCAGGGCT GCACAGCAGC AGCAAGGTGA CAATACACGA ATGTGATGAA 
GGGTCAGAGC CTTTGGGATT CTGGGATGCA TTAGGAAGGC GAGATCGAAA GGCCTATGAT 
TGCATGTTGC AAGATCCGGG AAAGTTTAAT TTCACCCCCC GCCTCTTCAG CCTCAGTAGT 
TCTTCGGGAG AATTCTCAGC CACTGAGTAC GTTTACCCTT CAAGAGACCC TACTGTCATC 
AATTCTATGC CCTTCTTGCA AGAGGATCTT TACACTGCCC CACAGCCAGC ACTGTTCCTT 
GTTGACAATC ACCATGAAGT GTACCTGTGG CAAGGCTGGT GGCCTGTGGA AAACAAAATC 
ACTGGATCAG CTCGAATCAG GTGGGCTACA GACAGGAAAT GCGCCATGGA GACCGTGCTC 
CAGTACTGCA AAGGAAAAAA TGTAAAGAAG CCCCCAAAGT CCTATCTCAT TCATGCCGGC 
CTAGAGCCTC TGACCTTCAC TAATATGTTC CCAAGTTGGG AACACAGGGA AGACATTGCA 
GAGATCACAG AAATGGATGC TGACGTTTCT AATCAGATAA TCCTTGTAGA AGATGTGCTG 
GCCAAGCTGT GTAAAACTGT GTATCCCCTA GCTGATCTCT TAGCCAGGCC TTTACCTGAG 
GGAGTTGATC CTCTGAAGCT TGAAATTTAT CTTTCAGATG AGGACTTTGA GGTTGCATTA 
GAGATGACAA GAGAAGAGTA CAATGCGCTG CCATCTTGGA AGCAGGTTAA TCTGAAAAAG 
GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137585.1
CDS	17..6694
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282099.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282099.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGCGAATCT 
CGGTTTGAGA TGTCAACGTC AGGCAGCGCT TTGTCTGCCG GTGGTGACCG TGAAAGAGGG 
CCGCGAAGGC CGAGGCGCTA TTTCACTCCT GGTGAAAATA GAAAGACTTC TGAGCGATTT 
AAAACCCAAC CTATAACGTC AGCAGAACGA AAGGAGACGG ATAGGTCTAT TATGTGTGCT 
GAAATACCAA CTGCTGACGA TGAAGAAAAA TTAGATGACC GAGCCAAACT AAGTGTAGCT 
GCAAAGAGAC TGCTTTTTAG AGAAATGGAA AAATCTTTTG AAATGAAGAA TATTCCTAAA 
CCACCTACGA GAAGCTCAGC AGTAGAGAGA AGAATGCGGC GTTTGCAGGA CAGATCACAC 
ACGCAGCCTA TTACCAGTGA GGAAGTGGTC ATTGCAGCTA CTGAAACTAC CCCTGCTTCA 
CGTTCTGTGA ATACCCACAC AGTAGTGGCA AGAGTACCTA GTCCCACTCT AGTTAAGAGC 
ACTGTACAAC CTACCAGCTT GCAGGCATCA GCACACCAAA AAATACTAGC TAAAGAGGAG 
ATAAGAGCAG CCAAAGAGGC GATGGGGCAA GATCAATCCG ATGAACCAGA TTCTTCCACA 
TTAAGCTTGG CAGAAAAGAT GGCTTTGTTT AACAAACTGT CTCAGCCAGT ATCCAGGGCT 
ATATCCACAA GGAGTCGGGG AGATATTAGG CACAGAAGGA TGAATACTCG TTACCAGACC 
CAGCCAGTCA CTCTTGGAGA AGTTGAGCAG ATGCAATGGA AAAAGAGTAA ACCGGTGCAA 
AACGAAAGTG GAAAACTAAC TCCCCTGTCA AGTACAGTTT CAACATCAGT TTCAGCAATG 
GCTTCTGCCC TTTCTGCACT GTTTGCTGGA GACATGCATG CGAAGTCACG TAGTGATGGA 
AGTGTGAGCG CTGCCACAAA AGATGAGTTG AGATTCCATG CTTCAGCAGA AAGCTCAGAC 
TCATCAGGAA AACGCACACA GAAGTCAGAA CAGTGGCAGC CCTCAATGGA AGTGCTGGAA 
AGCAAAAGAG CATCAAAGAA GCATGAAGAA GAAGGAAAGA GGTTTCTAGC ACATGAGGCT 
AATGAAATCA GAAAGTACAG CTCCTTTGAG GATGAAACCA CACATCCTGT TCTTGAAAAA 
ACAGGAGACT ACCATAAAGA GGCAGCCTAC AGCTTCCTGA GGAAGGGCAG CATGGAACTG 
TTTAGTTCAC AACCACTTTT TCAGCCTCTT GAAAGGAAGG AAATTGATAC CATCATGCAA 
GACCACGTGG AGCCTACCTC TGAACTCACC AGCACACCAG CAACGAGGAC ACTTTCCCAG 
ACTACAGCTG CAGCATCCTG TAGGCTACAG GAGCTGTCTG AACAACTGGA GGGAAAGTTC 
TACAAGAATT CCTGTGAGAT GTTTTCTGCT GGAGAGAACA AAATACAGAC AACTGAGGAT 
GCCACCGATA GCTCCAGCAA AACGATGTCA ATTAAAGAAA GGTTGGCATT GCTGAAGAAA 
AGTGGTGAAG AAGATTGGAA GAGCAGGCTC AGTAAAAAGC AGGAATATGC AAAAGTGTCT 
GCCACTGATC GTAGCGCACA GATGCAAGAA GTTGAGCAGC TGTTGAAAAA GGAACCTGTA 
TATGCTTCTA CTTACTCTCC TGTTATACCT GCTGTCCATA AATTTCATCC TTTTCTACCT 
ATTAATCAGC AGAGGATAGC AGATAATCAG GAGAGTCAAA TGACTATAGA AGAAAGAAAA 
CACCTCATCA CAGCTCGAGA AGAAGCCTGG AAGTCCAGAG GTAAAGGCGC AGCAAATGAC 
TCCACGCAGT TCACCGTAGC TGGTCGAATG GTAAAAAAAG GCCTGGCTTC TCCAAGTGCA 
CTAACACCAG TAGCATCCCC TTATGGTAAC AGGCAGAAGT CTACAACTCC GGTCACAAAG 
CCCTTGGAAG AAATTGAAGC TAGGCCTGAT ATGCAATTGG AATCAGATAT GAAGCTTGAC 
AAGCTGGAGT CATTCTTGGG AAGGCTGAAT AACAAAGTCG CTGGAATGCA AGAGACAGTG 
TTGACAGTTA CAGGAAAATC TGTGAAAGAG GTGATGAAGC TGGATGATGA TGAAACATTT 
TCCAAATTTT ACCGCCGTGT GGATTTCCCT TCCTCCTCTG TGCCTTTGGA CCTTGATGAG 
GACTTTGATG CCATCTTTGA CCCTTATGCA CCAAAGTTAA TATCCTCTGT AGCAGAGCAC 
AAACGTGCAG TGAGGCCTAT GCGCAGGGTT CAGTCCTCCA GAAACCCCCT GAAAATGCTG 
GCAGCAAGAG AAGATATTCT TCATGAGTAC ACTGAACAAA GACTGAATGT TGCCTTCATG 
GAGTCAAAGC GAATGAAAGT AGAAAAAATG TCTGCAAACT CAAATTTCTC AGAAGTAGCA 
CTTGCTGGTT TGGCAAGCAA GGAGAACTTC AGCAACGTTA GTCTGCGGAG TGTTAACCTA 
ACAGAGCAAA ATTCTAACAA CAGTGCTGTG CCATACAAGA AGTTAATGCT GCTGCAAATT 
AAAGGAAGAA GACATGTTCA AACAAGATTA GTTGAACCAA GGGCCTCATC ACTGAACAGT 
GGAGACTGTT TCCTATTGTT AACTCCACAT CTCTGTTTCT TATGGGTAGG AGAGTTTGCA 
AATGTCATAG AAAAAGCAAA GGCTTCAGAA CTTGCAACTT TGATTCAGAC AAAGAGGGAA 
CTTGGCTGTA GAGCTTCTTA TATACAGACT ATAGAAGAAG GAATAAACAC CCATACCCAT 
GCAGCCAAAG ACTTCTGGAA ACTCCTTGGT GGCCAAGCAA ATTACCAGTC TGCTGGAAGA 
CCTGAAGAGG ATGAAATGTA TGAAGCTGCC ATAATAGAAA CTAATTGCAT TTACCGTCTT 
GTAGAGGATA AACTCATTCC TGAAGATGAC TACTGGGGAA AGATGCCAAA ATGTACCCTG 
CTACAACCAA AAGAGGTGCT CGTATTTGAT TTTGGCAGTG AAGTGTATGT CTGGCATGGA 
AAAGAAGTTA CTTTAGCACA AAGAAAAGTG GCATTCCAGC TGGCAAAACA CTTATGGAAT 
GGCACCTTCG ACTACTCAAA TTGTGACATA AATCCTCTGG ACCCAGGAGA ATGCAATCCC 
CTCATACCCA GAAAGGGACA AGGACGTCCA GACTGGGCTG TGTTTGGCAG GCTCACAGAA 
CATAATGAGA CCATACTGTT CAAAGAAAAA TTCCTTGATT GGACAGAGCT GAAGAAACTC 
AATGAGAAGA ATTCTAGTGA ATCCCTTCAC CAGAAGGAAG AATCCAAATC TGATTCTAAA 
CCATATGATG TCATGCTAAT GGTACCTGTG CCCCAAACTG CTGTAGGCAC GGTTCTGGAT 
GGAATGAATA TTGGCCGGGG CTATGGACTT GTAGAAGGAG AAGATGGGAG GCAGTTTGAA 
ATTATCACTG CATCTGTGGA TGTCTGGCAC ATCCTGGAGT TTGATTATAG TAGATTGCCT 
AAACAAAGCA TAGGACAGTT CCATGAGGGA GACACATACG TGGTGAAGTG GAAGTACATG 
GTGAGCACTA CAGTTGGAAG CAGACAAAAA GGAGAGCAAC AAGTAAGAGC TGTTGGAAAA 
GAGAAATGTG TGTATTTCTT TTGGCAAGGA AGGCACTCCA CAGTGAGTGA GAAAGGGACA 
TCGGCACTGA TGACAGTGGA ACTTGATGAG GAGAGAGGAG CACAGGTACA AGTGCTTCAA 
GGGAAAGAAC TACCATGCTT CCTGCAGTGC TTCCAAGGAG GGATGATTGT GCATGCTGGA 
AGAAGGGAAG AGGAAGAAGA AAATGCACAA AGTGACTGGA GACTATATTG CGTGCGAGGA 
GAAGTTCCCA ATGAAGGAAA CTTACTTGAA GTAGCATGTC ACTGCAGCAG CCTGCGTTCC 
CGGACATCCA TGATTGTCCT CAATATAAAT AAAGCTCTTA TCTACCTGTG GCATGGCTGC 
AAAGCACAAT CTCACACCAA GGATGTAGGA AGAACAGCAG CCAATAAAAT AAAAGAACAA 
TGTCCGCTGG AAGCAGGGCT GCACAGCAGC AGCAAGGTGA CAATACACGA ATGTGATGAA 
GGGTCAGAGC CTTTGGGATT CTGGGATGCA TTAGGAAGGC GAGATCGAAA GGCCTATGAT 
TGCATGTTGC AAGATCCGGG AAAGTTTAAT TTCACCCCCC GCCTCTTCAG CCTCAGTAGT 
TCTTCGGGAG AATTCTCAGC CACTGAGTAC GTTTACCCTT CAAGAGACCC TACTGTCATC 
AATTCTATGC CCTTCTTGCA AGAGGATCTT TACACTGCCC CACAGCCAGC ACTGTTCCTT 
GTTGACAATC ACCATGAAGT GTACCTGTGG CAAGGCTGGT GGCCTGTGGA AAACAAAATC 
ACTGGATCAG CTCGAATCAG GTGGGCTACA GACAGGAAAT GCGCCATGGA GACCGTGCTC 
CAGTACTGCA AAGGAAAAAA TGTAAAGAAG CCCCCAAAGT CCTATCTCAT TCATGCCGGC 
CTAGAGCCTC TGACCTTCAC TAATATGTTC CCAAGTTGGG AACACAGGGA AGACATTGCA 
GAGATCACAG AAATGGATGC TGACGTTTCT AATCAGATAA TCCTTGTAGA AGATGTGCTG 
GCCAAGCTGT GTAAAACTGT GTATCCCCTA GCTGATCTCT TAGCCAGGCC TTTACCTGAG 
GGAGTTGATC CTCTGAAGCT TGAAATTTAT CTTTCAGATG AGGACTTTGA GGTTGCATTA 
GAGATGACAA GAGAAGAGTA CAATGCGCTG CCATCTTGGA AGCAGGTTAA TCTGAAAAAG 
GCAAAAGGAC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282100.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137586.1 Gallus gallus supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OGa31977
Clone ID Related Accession (Same CDS sequence)	XM_015282100.1 , XM_015282101.1 , XM_015282104.1 , XM_015282102.1 , XM_015282103.1
Accession Version	XM_015282100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137586.1
CDS	579..7211
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282100.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282100.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282101.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137587.1 Gallus gallus supervillin (SVIL), transcript variant X13, mRNA.

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CloneID	OGa31977
Clone ID Related Accession (Same CDS sequence)	XM_015282100.1 , XM_015282101.1 , XM_015282104.1 , XM_015282102.1 , XM_015282103.1
Accession Version	XM_015282101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137587.1
CDS	366..6998
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282101.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282101.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282104.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137590.1 Gallus gallus supervillin (SVIL), transcript variant X16, mRNA.

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CloneID	OGa31977
Clone ID Related Accession (Same CDS sequence)	XM_015282100.1 , XM_015282101.1 , XM_015282104.1 , XM_015282102.1 , XM_015282103.1
Accession Version	XM_015282104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137590.1
CDS	207..6839
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282104.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282104.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282102.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137588.1 Gallus gallus supervillin (SVIL), transcript variant X14, mRNA.

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CloneID	OGa31977
Clone ID Related Accession (Same CDS sequence)	XM_015282100.1 , XM_015282101.1 , XM_015282104.1 , XM_015282102.1 , XM_015282103.1
Accession Version	XM_015282102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137588.1
CDS	229..6861
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282102.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282102.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282103.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137589.1 Gallus gallus supervillin (SVIL), transcript variant X15, mRNA.

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CloneID	OGa31977
Clone ID Related Accession (Same CDS sequence)	XM_015282100.1 , XM_015282101.1 , XM_015282104.1 , XM_015282102.1 , XM_015282103.1
Accession Version	XM_015282103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137589.1
CDS	487..7119
Translation	MKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282103.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282103.1

ORF Insert Sequence:

ATGAAAAGAA AGGAGAGAAT AGCTAGACGC TTAGAGGGAA TTGAAAATGA TACGCAACCT 
ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282105.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137591.1 Gallus gallus supervillin (SVIL), transcript variant X17, mRNA.

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CloneID	OGa31978
Clone ID Related Accession (Same CDS sequence)	XM_015282105.1
Accession Version	XM_015282105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137591.1
CDS	581..7153
Translation	MLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282105.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282105.1

ORF Insert Sequence:

ATGCTTCTAC AGAACTGCCC AGGGTCAGTC ACACACCGTT TACTAGAAGA AGACACACCA 
AGGTATATGC GTGCAACTGA TCCATACAGT CATCACTTAG GAAGATCAAA TGAAGAGGAG 
GAAGCTTCAG ATTCTTCTGT AGAGAAACAA CCCAGATCAT CCAGATACCG CACAGAAACC 
ACAGGAGGAA ATACAGAGTC TTTGTACAGT ACAGGAAACA TGGATACCCA TGAACTAGAG 
TCTAAAGCAG AAAGAATAGC TAGGTACAAG GCAGAGAGAC GGCGCCAACT GGCAGAAAAG 
TATGGATTAT CTCTTGATTC TGATTTGGAT TCTGACTACT CATCCAGATA TTCCCGGGCA 
AGAAAAGATC CTGACAGTGT GGAAAGAAAG GCATTGAGAA GTGAAAGGCA TGATGATGAA 
AACAAGGACT CTGGCTCCTT GTATTTGTCC AGGACTGAGG TGAAGGAGTC AAAGAGTGTG 
CTTTCTGAGT CCAGGGAGTA CTCTAGCCGT GAAAAAGATG GTATTCCAGC TAAAGAAGAG 
CTTTCAAATG AAAAGAGTGA TAAAAGAGCA GATGATGACA GTGCCATTAG ACAGGCTTCT 
GACCCTTCAG CAACATTGGA TAGTTCTGTC TCACTAACAC TTTCTGGACG AGAGTCTTCC 
TCCTGTAATG AAGTGCCAAT ATCACCAAAG CAAAGCCCCC GAGAGTCTCT TTCTTCACCA 
AAGCGGGCAG CCTCACCAAT CCATTTGCAG AATGATCAGC CCTTGCACAG TAACATCAGA 
CAAAGTAGTG CAGGGAAAGC AAAACCTGAA TGGTTCCTTC AGAAAGATTC AGAAGGGGAC 
ACACCATCTC TTATCAGCTG GCCCTCCAGA GTAAAAGTTA GAGAGAAACT GGTGAAAGAG 
GAAAGTGCAC GTGGAAGCCC TGAACCTGTC ACAGACAGTG TATCTCAGAG AAAATCCCAG 
CCAGTGTCAG CCCACTACAT GCCTCTTCAG ACAGAAACGT CTGCCTTTGA TAGGGTTCTC 
AATCAGCCCG TCAGTGCAGT CCGCCAACCA ATACATGGGT ATATTCAGCC TGCTGGTGTT 
GCACACACGG CTCAGCTGAT GGCAACAGAA GAACCAGAAA GCATCTTCGT TGGCAGCCGC 
CTCCTTCCAG ACAGTGTCAG CCCCTTAACG AAATCAGCAG CAGCCACTGC ACCAGAGGGT 
GAAAAGAAAG AGACGCTGGC TTATGGAGCA AAGCCTGGCG AAGAAGAATC CCCAGAGGGT 
GAGAAGGCTT CCAAAGGCAG AAGACCAATT TTGCCATCTG AGATTCGCAA GCAAGGGAAG 
AGCCACGAAG GCCTCTTTGG AGGAGAAGAA CCAGATACCA GTGTGGGGAG CTCCTCATCT 
GCAAGCAAGC CGAAGGTTGG CTCAGATGTT CAGCAGGAGC AGGAATGCAA AAAGAAGCAG 
GCGCCTGAGC AGCAGCTGAA GACACCTGAG AACATGGAGA GGAGCGAAGC TGTCATGTAC 
ATACAGAGCG GGTCTGTATC AAAGCCAGCC CAGGCAAAGG GCCCCGTTTG GAAGGTGTCA 
AAGCATAAAG TCCTGACTCG CTCCATGTCT GACTATACTG TGCCTCCTAA GCTAGAAGCT 
TTTCATAGTA AGAGCATGGA AGCAAACGCA GCAAATGGTG AGATTGGCAT GGTTGACACA 
AAGGTCTCTG TTGCCCAGCT CCGTAATGTC TTTCTGGAAA CTGCTCATGC CAACAAGAAA 
ACGGACCTCG AATCTCGGTT TGAGATGTCA ACGTCAGGCA GCGCTTTGTC TGCCGGTGGT 
GACCGTGAAA GAGGGCCGCG AAGGCCGAGG CGCTATTTCA CTCCTGGTGA AAATAGAAAG 
ACTTCTGAGC GATTTAAAAC CCAACCTATA ACGTCAGCAG AACGAAAGGA GACGGATAGG 
TCTATTATGT GTGCTGAAAT ACCAACTGCT GACGATGAAG AAAAATTAGA TGACCGAGCC 
AAACTAAGTG TAGCTGCAAA GAGACTGCTT TTTAGAGAAA TGGAAAAATC TTTTGAAATG 
AAGAATATTC CTAAACCACC TACGAGAAGC TCAGCAGTAG AGAGAAGAAT GCGGCGTTTG 
CAGGACAGAT CACACACGCA GCCTATTACC AGTGAGGAAG TGGTCATTGC AGCTACTGAA 
ACTACCCCTG CTTCACGTTC TGTGAATACC CACACAGTAG TGGCAAGAGT ACCTAGTCCC 
ACTCTAGTTA AGAGCACTGT ACAACCTACC AGCTTGCAGG CATCAGCACA CCAAAAAATA 
CTAGCTAAAG AGGAGATAAG AGCAGCCAAA GAGGCGATGG GGCAAGATCA ATCCGATGAA 
CCAGATTCTT CCACATTAAG CTTGGCAGAA AAGATGGCTT TGTTTAACAA ACTGTCTCAG 
CCAGTATCCA GGGCTATATC CACAAGGAGT CGGGGAGATA TTAGGCACAG AAGGATGAAT 
ACTCGTTACC AGACCCAGCC AGTCACTCTT GGAGAAGTTG AGCAGATGCA ATGGAAAAAG 
AGTAAACCGG TGCAAAACGA AAGTGGAAAA CTAACTCCCC TGTCAAGTAC AGTTTCAACA 
TCAGTTTCAG CAATGGCTTC TGCCCTTTCT GCACTGTTTG CTGGAGACAT GCATGCGAAG 
TCACGTAGTG ATGGAAGTGT GAGCGCTGCC ACAAAAGATG AGTTGAGATT CCATGCTTCA 
GCAGAAAGCT CAGACTCATC AGGAAAACGC ACACAGAAGT CAGAACAGTG GCAGCCCTCA 
ATGGAAGTGC TGGAAAGCAA AAGAGCATCA AAGAAGCATG AAGAAGAAGG AAAGAGGTTT 
CTAGCACATG AGGCTAATGA AATCAGAAAG TACAGCTCCT TTGAGGATGA AACCACACAT 
CCTGTTCTTG AAAAAACAGG AGACTACCAT AAAGAGGCAG CCTACAGCTT CCTGAGGAAG 
GGCAGCATGG AACTGTTTAG TTCACAACCA CTTTTTCAGC CTCTTGAAAG GAAGGAAATT 
GATACCATCA TGCAAGACCA CGTGGAGCCT ACCTCTGAAC TCACCAGCAC ACCAGCAACG 
AGGACACTTT CCCAGACTAC AGCTGCAGCA TCCTGTAGGC TACAGGAGCT GTCTGAACAA 
CTGGAGGGAA AGTTCTACAA GAATTCCTGT GAGATGTTTT CTGCTGGAGA GAACAAAATA 
CAGACAACTG AGGATGCCAC CGATAGCTCC AGCAAAACGA TGTCAATTAA AGAAAGGTTG 
GCATTGCTGA AGAAAAGTGG TGAAGAAGAT TGGAAGAGCA GGCTCAGTAA AAAGCAGGAA 
TATGCAAAAG TGTCTGCCAC TGATCGTAGC GCACAGATGC AAGAAGTTGA GCAGCTGTTG 
AAAAAGGAAC CTGTATATGC TTCTACTTAC TCTCCTGTTA TACCTGCTGT CCATAAATTT 
CATCCTTTTC TACCTATTAA TCAGCAGAGG ATAGCAGATA ATCAGGAGAG TCAAATGACT 
ATAGAAGAAA GAAAACACCT CATCACAGCT CGAGAAGAAG CCTGGAAGTC CAGAGGTAAA 
GGCGCAGCAA ATGACTCCAC GCAGTTCACC GTAGCTGGTC GAATGGTAAA AAAAGGCCTG 
GCTTCTCCAA GTGCACTAAC ACCAGTAGCA TCCCCTTATG GTAACAGGCA GAAGTCTACA 
ACTCCGGTCA CAAAGCCCTT GGAAGAAATT GAAGCTAGGC CTGATATGCA ATTGGAATCA 
GATATGAAGC TTGACAAGCT GGAGTCATTC TTGGGAAGGC TGAATAACAA AGTCGCTGGA 
ATGCAAGAGA CAGTGTTGAC AGTTACAGGA AAATCTGTGA AAGAGGTGAT GAAGCTGGAT 
GATGATGAAA CATTTTCCAA ATTTTACCGC CGTGTGGATT TCCCTTCCTC CTCTGTGCCT 
TTGGACCTTG ATGAGGACTT TGATGCCATC TTTGACCCTT ATGCACCAAA GTTAATATCC 
TCTGTAGCAG AGCACAAACG TGCAGTGAGG CCTATGCGCA GGGTTCAGTC CTCCAGAAAC 
CCCCTGAAAA TGCTGGCAGC AAGAGAAGAT ATTCTTCATG AGTACACTGA ACAAAGACTG 
AATGTTGCCT TCATGGAGTC AAAGCGAATG AAAGTAGAAA AAATGTCTGC AAACTCAAAT 
TTCTCAGAAG TAGCACTTGC TGGTTTGGCA AGCAAGGAGA ACTTCAGCAA CGTTAGTCTG 
CGGAGTGTTA ACCTAACAGA GCAAAATTCT AACAACAGTG CTGTGCCATA CAAGAAGTTA 
ATGCTGCTGC AAATTAAAGG AAGAAGACAT GTTCAAACAA GATTAGTTGA ACCAAGGGCC 
TCATCACTGA ACAGTGGAGA CTGTTTCCTA TTGTTAACTC CACATCTCTG TTTCTTATGG 
GTAGGAGAGT TTGCAAATGT CATAGAAAAA GCAAAGGCTT CAGAACTTGC AACTTTGATT 
CAGACAAAGA GGGAACTTGG CTGTAGAGCT TCTTATATAC AGACTATAGA AGAAGGAATA 
AACACCCATA CCCATGCAGC CAAAGACTTC TGGAAACTCC TTGGTGGCCA AGCAAATTAC 
CAGTCTGCTG GAAGACCTGA AGAGGATGAA ATGTATGAAG CTGCCATAAT AGAAACTAAT 
TGCATTTACC GTCTTGTAGA GGATAAACTC ATTCCTGAAG ATGACTACTG GGGAAAGATG 
CCAAAATGTA CCCTGCTACA ACCAAAAGAG GTGCTCGTAT TTGATTTTGG CAGTGAAGTG 
TATGTCTGGC ATGGAAAAGA AGTTACTTTA GCACAAAGAA AAGTGGCATT CCAGCTGGCA 
AAACACTTAT GGAATGGCAC CTTCGACTAC TCAAATTGTG ACATAAATCC TCTGGACCCA 
GGAGAATGCA ATCCCCTCAT ACCCAGAAAG GGACAAGGAC GTCCAGACTG GGCTGTGTTT 
GGCAGGCTCA CAGAACATAA TGAGACCATA CTGTTCAAAG AAAAATTCCT TGATTGGACA 
GAGCTGAAGA AACTCAATGA GAAGAATTCT AGTGAATCCC TTCACCAGAA GGAAGAATCC 
AAATCTGATT CTAAACCATA TGATGTCATG CTAATGGTAC CTGTGCCCCA AACTGCTGTA 
GGCACGGTTC TGGATGGAAT GAATATTGGC CGGGGCTATG GACTTGTAGA AGGAGAAGAT 
GGGAGGCAGT TTGAAATTAT CACTGCATCT GTGGATGTCT GGCACATCCT GGAGTTTGAT 
TATAGTAGAT TGCCTAAACA AAGCATAGGA CAGTTCCATG AGGGAGACAC ATACGTGGTG 
AAGTGGAAGT ACATGGTGAG CACTACAGTT GGAAGCAGAC AAAAAGGAGA GCAACAAGTA 
AGAGCTGTTG GAAAAGAGAA ATGTGTGTAT TTCTTTTGGC AAGGAAGGCA CTCCACAGTG 
AGTGAGAAAG GGACATCGGC ACTGATGACA GTGGAACTTG ATGAGGAGAG AGGAGCACAG 
GTACAAGTGC TTCAAGGGAA AGAACTACCA TGCTTCCTGC AGTGCTTCCA AGGAGGGATG 
ATTGTGCATG CTGGAAGAAG GGAAGAGGAA GAAGAAAATG CACAAAGTGA CTGGAGACTA 
TATTGCGTGC GAGGAGAAGT TCCCAATGAA GGAAACTTAC TTGAAGTAGC ATGTCACTGC 
AGCAGCCTGC GTTCCCGGAC ATCCATGATT GTCCTCAATA TAAATAAAGC TCTTATCTAC 
CTGTGGCATG GCTGCAAAGC ACAATCTCAC ACCAAGGATG TAGGAAGAAC AGCAGCCAAT 
AAAATAAAAG AACAATGTCC GCTGGAAGCA GGGCTGCACA GCAGCAGCAA GGTGACAATA 
CACGAATGTG ATGAAGGGTC AGAGCCTTTG GGATTCTGGG ATGCATTAGG AAGGCGAGAT 
CGAAAGGCCT ATGATTGCAT GTTGCAAGAT CCGGGAAAGT TTAATTTCAC CCCCCGCCTC 
TTCAGCCTCA GTAGTTCTTC GGGAGAATTC TCAGCCACTG AGTACGTTTA CCCTTCAAGA 
GACCCTACTG TCATCAATTC TATGCCCTTC TTGCAAGAGG ATCTTTACAC TGCCCCACAG 
CCAGCACTGT TCCTTGTTGA CAATCACCAT GAAGTGTACC TGTGGCAAGG CTGGTGGCCT 
GTGGAAAACA AAATCACTGG ATCAGCTCGA ATCAGGTGGG CTACAGACAG GAAATGCGCC 
ATGGAGACCG TGCTCCAGTA CTGCAAAGGA AAAAATGTAA AGAAGCCCCC AAAGTCCTAT 
CTCATTCATG CCGGCCTAGA GCCTCTGACC TTCACTAATA TGTTCCCAAG TTGGGAACAC 
AGGGAAGACA TTGCAGAGAT CACAGAAATG GATGCTGACG TTTCTAATCA GATAATCCTT 
GTAGAAGATG TGCTGGCCAA GCTGTGTAAA ACTGTGTATC CCCTAGCTGA TCTCTTAGCC 
AGGCCTTTAC CTGAGGGAGT TGATCCTCTG AAGCTTGAAA TTTATCTTTC AGATGAGGAC 
TTTGAGGTTG CATTAGAGAT GACAAGAGAA GAGTACAATG CGCTGCCATC TTGGAAGCAG 
GTTAATCTGA AAAAGGCAAA AGGACTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282106.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137592.1 Gallus gallus supervillin (SVIL), transcript variant X18, mRNA.

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CloneID	OGa31979
Clone ID Related Accession (Same CDS sequence)	XM_015282106.1
Accession Version	XM_015282106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTATT 
ATGTGTGCTG AAATACCAAC TGCTGACGAT GAAGAAAAAT TAGATGACCG AGCCAAACTA 
AGTGTAGCTG CAAAGAGACT GCTTTTTAGA GAAATGGAAA AATCTTTTGA AATGAAGAAT 
ATTCCTAAAC CACCTACGAG AAGCTCAGCA GTAGAGAGAA GAATGCGGCG TTTGCAGGAC 
AGATCACACA CGCAGCCTAT TACCAGTGAG GAAGTGGTCA TTGCAGCTAC TGAAACTACC 
CCTGCTTCAC GTTCTGTGAA TACCCACACA GTAGTGGCAA GAGTACCTAG TCCCACTCTA 
GTTAAGAGCA CTGTACAACC TACCAGCTTG CAGGCATCAG CACACCAAAA AATACTAGCT 
AAAGAGGAGA TAAGAGCAGC CAAAGAGGCG ATGGGGCAAG ATCAATCCGA TGAACCAGAT 
TCTTCCACAT TAAGCTTGGC AGAAAAGATG GCTTTGTTTA ACAAACTGTC TCAGCCAGTA 
TCCAGGGCTA TATCCACAAG GAGTCGGGGA GATATTAGGC ACAGAAGGAT GAATACTCGT 
TACCAGACCC AGCCAGTCAC TCTTGGAGAA GTTGAGCAGA TGCAATGGAA AAAGAGTAAA 
CCGGTGCAAA ACGAAAGTGG AAAACTAACT CCCCTGTCAA GTACAGTTTC AACATCAGTT 
TCAGCAATGG CTTCTGCCCT TTCTGCACTG TTTGCTGGAG ACATGCATGC GAAGTCACGT 
AGTGATGGAA GTGTGAGCGC TGCCACAAAA GATGAGTTGA GATTCCATGC TTCAGCAGAA 
AGCTCAGACT CATCAGGAAA ACGCACACAG AAGTCAGAAC AGTGGCAGCC CTCAATGGAA 
GTGCTGGAAA GCAAAAGAGC ATCAAAGAAG CATGAAGAAG AAGGAAAGAG GTTTCTAGCA 
CATGAGGCTA ATGAAATCAG AAAGTACAGC TCCTTTGAGG ATGAAACCAC ACATCCTGTT 
CTTGAAAAAA CAGGAGACTA CCATAAAGAG GCAGCCTACA GCTTCCTGAG GAAGGGCAGC 
ATGGAACTGT TTAGTTCACA ACCACTTTTT CAGCCTCTTG AAAGGAAGGA AATTGATACC 
ATCATGCAAG ACCACGTGGA GCCTACCTCT GAACTCACCA GCACACCAGC AACGAGGACA 
CTTTCCCAGA CTACAGCTGC AGCATCCTGT AGGCTACAGG AGCTGTCTGA ACAACTGGAG 
GGAAAGTTCT ACAAGAATTC CTGTGAGATG TTTTCTGCTG GAGAGAACAA AATACAGACA 
ACTGAGGATG CCACCGATAG CTCCAGCAAA ACGATGTCAA TTAAAGAAAG GTTGGCATTG 
CTGAAGAAAA GTGGTGAAGA AGATTGGAAG AGCAGGCTCA GTAAAAAGCA GGAATATGCA 
AAAGTGTCTG CCACTGATCG TAGCGCACAG ATGCAAGAAG TTGAGCAGCT GTTGAAAAAG 
GAACCTGTAT ATGCTTCTAC TTACTCTCCT GTTATACCTG CTGTCCATAA ATTTCATCCT 
TTTCTACCTA TTAATCAGCA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137592.1
CDS	17..6523
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282106.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282106.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTATT 
ATGTGTGCTG AAATACCAAC TGCTGACGAT GAAGAAAAAT TAGATGACCG AGCCAAACTA 
AGTGTAGCTG CAAAGAGACT GCTTTTTAGA GAAATGGAAA AATCTTTTGA AATGAAGAAT 
ATTCCTAAAC CACCTACGAG AAGCTCAGCA GTAGAGAGAA GAATGCGGCG TTTGCAGGAC 
AGATCACACA CGCAGCCTAT TACCAGTGAG GAAGTGGTCA TTGCAGCTAC TGAAACTACC 
CCTGCTTCAC GTTCTGTGAA TACCCACACA GTAGTGGCAA GAGTACCTAG TCCCACTCTA 
GTTAAGAGCA CTGTACAACC TACCAGCTTG CAGGCATCAG CACACCAAAA AATACTAGCT 
AAAGAGGAGA TAAGAGCAGC CAAAGAGGCG ATGGGGCAAG ATCAATCCGA TGAACCAGAT 
TCTTCCACAT TAAGCTTGGC AGAAAAGATG GCTTTGTTTA ACAAACTGTC TCAGCCAGTA 
TCCAGGGCTA TATCCACAAG GAGTCGGGGA GATATTAGGC ACAGAAGGAT GAATACTCGT 
TACCAGACCC AGCCAGTCAC TCTTGGAGAA GTTGAGCAGA TGCAATGGAA AAAGAGTAAA 
CCGGTGCAAA ACGAAAGTGG AAAACTAACT CCCCTGTCAA GTACAGTTTC AACATCAGTT 
TCAGCAATGG CTTCTGCCCT TTCTGCACTG TTTGCTGGAG ACATGCATGC GAAGTCACGT 
AGTGATGGAA GTGTGAGCGC TGCCACAAAA GATGAGTTGA GATTCCATGC TTCAGCAGAA 
AGCTCAGACT CATCAGGAAA ACGCACACAG AAGTCAGAAC AGTGGCAGCC CTCAATGGAA 
GTGCTGGAAA GCAAAAGAGC ATCAAAGAAG CATGAAGAAG AAGGAAAGAG GTTTCTAGCA 
CATGAGGCTA ATGAAATCAG AAAGTACAGC TCCTTTGAGG ATGAAACCAC ACATCCTGTT 
CTTGAAAAAA CAGGAGACTA CCATAAAGAG GCAGCCTACA GCTTCCTGAG GAAGGGCAGC 
ATGGAACTGT TTAGTTCACA ACCACTTTTT CAGCCTCTTG AAAGGAAGGA AATTGATACC 
ATCATGCAAG ACCACGTGGA GCCTACCTCT GAACTCACCA GCACACCAGC AACGAGGACA 
CTTTCCCAGA CTACAGCTGC AGCATCCTGT AGGCTACAGG AGCTGTCTGA ACAACTGGAG 
GGAAAGTTCT ACAAGAATTC CTGTGAGATG TTTTCTGCTG GAGAGAACAA AATACAGACA 
ACTGAGGATG CCACCGATAG CTCCAGCAAA ACGATGTCAA TTAAAGAAAG GTTGGCATTG 
CTGAAGAAAA GTGGTGAAGA AGATTGGAAG AGCAGGCTCA GTAAAAAGCA GGAATATGCA 
AAAGTGTCTG CCACTGATCG TAGCGCACAG ATGCAAGAAG TTGAGCAGCT GTTGAAAAAG 
GAACCTGTAT ATGCTTCTAC TTACTCTCCT GTTATACCTG CTGTCCATAA ATTTCATCCT 
TTTCTACCTA TTAATCAGCA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282111.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137597.1 Gallus gallus supervillin (SVIL), transcript variant X25, mRNA.

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CloneID	OGa31984
Clone ID Related Accession (Same CDS sequence)	XM_015282111.2 , XM_015282111.1
Accession Version	XM_015282111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGGTGCAA AACGAAAGTG GAAAACTAAC TCCCCTGTCA 
AGTACAGTTT CAACATCAGT TTCAGCAATG GCTTCTGCCC TTTCTGCACT GTTTGCTGGA 
GACATGCATG CGAAGTCACG TAGTGATGGA AGTGTGAGCG CTGCCACAAA AGATGAGTTG 
AGATTCCATG CTTCAGCAGA AAGCTCAGAC TCATCAGGAA AACGCACACA GAAGTCAGAA 
CAGTGGCAGC CCTCAATGGA AGTGCTGGAA AGCAAAAGAG CATCAAAGAA GCATGAAGAA 
GAAGGAAAGA GGTTTCTAGC ACATGAGGCT AATGAAATCA GAAAGTACAG CTCCTTTGAG 
GATGAAACCA CACATCCTGT TCTTGAAAAA ACAGGAGACT ACCATAAAGA GGCAGCCTAC 
AGCTTCCTGA GGAAGGGCAG CATGGAACTG TTTAGTTCAC AACCACTTTT TCAGCCTCTT 
GAAAGGAAGG AAATTGATAC CATCATGCAA GACCACGTGG AGCCTACCTC TGAACTCACC 
AGCACACCAG CAACGAGGAC ACTTTCCCAG ACTACAGCTG CAGCATCCTG TAGGCTACAG 
GAGCTGTCTG AACAACTGGA GGGAAAGTTC TACAAGAATT CCTGTGAGAT GTTTTCTGCT 
GGAGAGAACA AAATACAGAC AACTGAGGAT GCCACCGATA GCTCCAGCAA AACGATGTCA 
ATTAAAGAAA GGTTGGCATT GCTGAAGAAA AGTGGTGAAG AAGATTGGAA GAGCAGGCTC 
AGTAAAAAGC AGGAATATGC AAAAGTGTCT GCCACTGATC GTAGCGCACA GATGCAAGAA 
GTTGAGCAGC TGTTGAAAAA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137597.1
CDS	19..6825
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282111.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282111.2

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGGTGCAA AACGAAAGTG GAAAACTAAC TCCCCTGTCA 
AGTACAGTTT CAACATCAGT TTCAGCAATG GCTTCTGCCC TTTCTGCACT GTTTGCTGGA 
GACATGCATG CGAAGTCACG TAGTGATGGA AGTGTGAGCG CTGCCACAAA AGATGAGTTG 
AGATTCCATG CTTCAGCAGA AAGCTCAGAC TCATCAGGAA AACGCACACA GAAGTCAGAA 
CAGTGGCAGC CCTCAATGGA AGTGCTGGAA AGCAAAAGAG CATCAAAGAA GCATGAAGAA 
GAAGGAAAGA GGTTTCTAGC ACATGAGGCT AATGAAATCA GAAAGTACAG CTCCTTTGAG 
GATGAAACCA CACATCCTGT TCTTGAAAAA ACAGGAGACT ACCATAAAGA GGCAGCCTAC 
AGCTTCCTGA GGAAGGGCAG CATGGAACTG TTTAGTTCAC AACCACTTTT TCAGCCTCTT 
GAAAGGAAGG AAATTGATAC CATCATGCAA GACCACGTGG AGCCTACCTC TGAACTCACC 
AGCACACCAG CAACGAGGAC ACTTTCCCAG ACTACAGCTG CAGCATCCTG TAGGCTACAG 
GAGCTGTCTG AACAACTGGA GGGAAAGTTC TACAAGAATT CCTGTGAGAT GTTTTCTGCT 
GGAGAGAACA AAATACAGAC AACTGAGGAT GCCACCGATA GCTCCAGCAA AACGATGTCA 
ATTAAAGAAA GGTTGGCATT GCTGAAGAAA AGTGGTGAAG AAGATTGGAA GAGCAGGCTC 
AGTAAAAAGC AGGAATATGC AAAAGTGTCT GCCACTGATC GTAGCGCACA GATGCAAGAA 
GTTGAGCAGC TGTTGAAAAA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282111.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137597.1 Gallus gallus supervillin (SVIL), transcript variant X23, mRNA.

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CloneID	OGa31984
Clone ID Related Accession (Same CDS sequence)	XM_015282111.2 , XM_015282111.1
Accession Version	XM_015282111.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGGTGCAA AACGAAAGTG GAAAACTAAC TCCCCTGTCA 
AGTACAGTTT CAACATCAGT TTCAGCAATG GCTTCTGCCC TTTCTGCACT GTTTGCTGGA 
GACATGCATG CGAAGTCACG TAGTGATGGA AGTGTGAGCG CTGCCACAAA AGATGAGTTG 
AGATTCCATG CTTCAGCAGA AAGCTCAGAC TCATCAGGAA AACGCACACA GAAGTCAGAA 
CAGTGGCAGC CCTCAATGGA AGTGCTGGAA AGCAAAAGAG CATCAAAGAA GCATGAAGAA 
GAAGGAAAGA GGTTTCTAGC ACATGAGGCT AATGAAATCA GAAAGTACAG CTCCTTTGAG 
GATGAAACCA CACATCCTGT TCTTGAAAAA ACAGGAGACT ACCATAAAGA GGCAGCCTAC 
AGCTTCCTGA GGAAGGGCAG CATGGAACTG TTTAGTTCAC AACCACTTTT TCAGCCTCTT 
GAAAGGAAGG AAATTGATAC CATCATGCAA GACCACGTGG AGCCTACCTC TGAACTCACC 
AGCACACCAG CAACGAGGAC ACTTTCCCAG ACTACAGCTG CAGCATCCTG TAGGCTACAG 
GAGCTGTCTG AACAACTGGA GGGAAAGTTC TACAAGAATT CCTGTGAGAT GTTTTCTGCT 
GGAGAGAACA AAATACAGAC AACTGAGGAT GCCACCGATA GCTCCAGCAA AACGATGTCA 
ATTAAAGAAA GGTTGGCATT GCTGAAGAAA AGTGGTGAAG AAGATTGGAA GAGCAGGCTC 
AGTAAAAAGC AGGAATATGC AAAAGTGTCT GCCACTGATC GTAGCGCACA GATGCAAGAA 
GTTGAGCAGC TGTTGAAAAA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137597.1
CDS	17..6823
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282111.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282111.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACTGAAA CTACCCCTGC TTCACGTTCT 
GTGAATACCC ACACAGTAGT GGCAAGAGTA CCTAGTCCCA CTCTAGTTAA GAGCACTGTA 
CAACCTACCA GCTTGCAGGC ATCAGCACAC CAAAAAATAC TAGCTAAAGA GGAGATAAGA 
GCAGCCAAAG AGGCGATGGG GCAAGATCAA TCCGATGAAC CAGATTCTTC CACATTAAGC 
TTGGCAGAAA AGATGGCTTT GTTTAACAAA CTGTCTCAGC CAGTATCCAG GGCTATATCC 
ACAAGGAGTC GGGGAGATAT TAGGCACAGA AGGATGAATA CTCGTTACCA GACCCAGCCA 
GTCACTCTTG GAGAAGTTGA GCAGGTGCAA AACGAAAGTG GAAAACTAAC TCCCCTGTCA 
AGTACAGTTT CAACATCAGT TTCAGCAATG GCTTCTGCCC TTTCTGCACT GTTTGCTGGA 
GACATGCATG CGAAGTCACG TAGTGATGGA AGTGTGAGCG CTGCCACAAA AGATGAGTTG 
AGATTCCATG CTTCAGCAGA AAGCTCAGAC TCATCAGGAA AACGCACACA GAAGTCAGAA 
CAGTGGCAGC CCTCAATGGA AGTGCTGGAA AGCAAAAGAG CATCAAAGAA GCATGAAGAA 
GAAGGAAAGA GGTTTCTAGC ACATGAGGCT AATGAAATCA GAAAGTACAG CTCCTTTGAG 
GATGAAACCA CACATCCTGT TCTTGAAAAA ACAGGAGACT ACCATAAAGA GGCAGCCTAC 
AGCTTCCTGA GGAAGGGCAG CATGGAACTG TTTAGTTCAC AACCACTTTT TCAGCCTCTT 
GAAAGGAAGG AAATTGATAC CATCATGCAA GACCACGTGG AGCCTACCTC TGAACTCACC 
AGCACACCAG CAACGAGGAC ACTTTCCCAG ACTACAGCTG CAGCATCCTG TAGGCTACAG 
GAGCTGTCTG AACAACTGGA GGGAAAGTTC TACAAGAATT CCTGTGAGAT GTTTTCTGCT 
GGAGAGAACA AAATACAGAC AACTGAGGAT GCCACCGATA GCTCCAGCAA AACGATGTCA 
ATTAAAGAAA GGTTGGCATT GCTGAAGAAA AGTGGTGAAG AAGATTGGAA GAGCAGGCTC 
AGTAAAAAGC AGGAATATGC AAAAGTGTCT GCCACTGATC GTAGCGCACA GATGCAAGAA 
GTTGAGCAGC TGTTGAAAAA GAGGATAGCA GATAATCAGG AGAGTCAAAT GACTATAGAA 
GAAAGAAAAC ACCTCATCAC AGCTCGAGAA GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA 
GCAAATGACT CCACGCAGTT CACCGTAGCT GGTCGAATGG TAAAAAAAGG CCTGGCTTCT 
CCAAGTGCAC TAACACCAGT AGCATCCCCT TATGGTAACA GGCAGAAGTC TACAACTCCG 
GTCACAAAGC CCTTGGAAGA AATTGAAGCT AGGCCTGATA TGCAATTGGA ATCAGATATG 
AAGCTTGACA AGCTGGAGTC ATTCTTGGGA AGGCTGAATA ACAAAGTCGC TGGAATGCAA 
GAGACAGTGT TGACAGTTAC AGGAAAATCT GTGAAAGAGG TGATGAAGCT GGATGATGAT 
GAAACATTTT CCAAATTTTA CCGCCGTGTG GATTTCCCTT CCTCCTCTGT GCCTTTGGAC 
CTTGATGAGG ACTTTGATGC CATCTTTGAC CCTTATGCAC CAAAGTTAAT ATCCTCTGTA 
GCAGAGCACA AACGTGCAGT GAGGCCTATG CGCAGGGTTC AGTCCTCCAG AAACCCCCTG 
AAAATGCTGG CAGCAAGAGA AGATATTCTT CATGAGTACA CTGAACAAAG ACTGAATGTT 
GCCTTCATGG AGTCAAAGCG AATGAAAGTA GAAAAAATGT CTGCAAACTC AAATTTCTCA 
GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG GAGAACTTCA GCAACGTTAG TCTGCGGAGT 
GTTAACCTAA CAGAGCAAAA TTCTAACAAC AGTGCTGTGC CATACAAGAA GTTAATGCTG 
CTGCAAATTA AAGGAAGAAG ACATGTTCAA ACAAGATTAG TTGAACCAAG GGCCTCATCA 
CTGAACAGTG GAGACTGTTT CCTATTGTTA ACTCCACATC TCTGTTTCTT ATGGGTAGGA 
GAGTTTGCAA ATGTCATAGA AAAAGCAAAG GCTTCAGAAC TTGCAACTTT GATTCAGACA 
AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT ATACAGACTA TAGAAGAAGG AATAAACACC 
CATACCCATG CAGCCAAAGA CTTCTGGAAA CTCCTTGGTG GCCAAGCAAA TTACCAGTCT 
GCTGGAAGAC CTGAAGAGGA TGAAATGTAT GAAGCTGCCA TAATAGAAAC TAATTGCATT 
TACCGTCTTG TAGAGGATAA ACTCATTCCT GAAGATGACT ACTGGGGAAA GATGCCAAAA 
TGTACCCTGC TACAACCAAA AGAGGTGCTC GTATTTGATT TTGGCAGTGA AGTGTATGTC 
TGGCATGGAA AAGAAGTTAC TTTAGCACAA AGAAAAGTGG CATTCCAGCT GGCAAAACAC 
TTATGGAATG GCACCTTCGA CTACTCAAAT TGTGACATAA ATCCTCTGGA CCCAGGAGAA 
TGCAATCCCC TCATACCCAG AAAGGGACAA GGACGTCCAG ACTGGGCTGT GTTTGGCAGG 
CTCACAGAAC ATAATGAGAC CATACTGTTC AAAGAAAAAT TCCTTGATTG GACAGAGCTG 
AAGAAACTCA ATGAGAAGAA TTCTAGTGAA TCCCTTCACC AGAAGGAAGA ATCCAAATCT 
GATTCTAAAC CATATGATGT CATGCTAATG GTACCTGTGC CCCAAACTGC TGTAGGCACG 
GTTCTGGATG GAATGAATAT TGGCCGGGGC TATGGACTTG TAGAAGGAGA AGATGGGAGG 
CAGTTTGAAA TTATCACTGC ATCTGTGGAT GTCTGGCACA TCCTGGAGTT TGATTATAGT 
AGATTGCCTA AACAAAGCAT AGGACAGTTC CATGAGGGAG ACACATACGT GGTGAAGTGG 
AAGTACATGG TGAGCACTAC AGTTGGAAGC AGACAAAAAG GAGAGCAACA AGTAAGAGCT 
GTTGGAAAAG AGAAATGTGT GTATTTCTTT TGGCAAGGAA GGCACTCCAC AGTGAGTGAG 
AAAGGGACAT CGGCACTGAT GACAGTGGAA CTTGATGAGG AGAGAGGAGC ACAGGTACAA 
GTGCTTCAAG GGAAAGAACT ACCATGCTTC CTGCAGTGCT TCCAAGGAGG GATGATTGTG 
CATGCTGGAA GAAGGGAAGA GGAAGAAGAA AATGCACAAA GTGACTGGAG ACTATATTGC 
GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC TTACTTGAAG TAGCATGTCA CTGCAGCAGC 
CTGCGTTCCC GGACATCCAT GATTGTCCTC AATATAAATA AAGCTCTTAT CTACCTGTGG 
CATGGCTGCA AAGCACAATC TCACACCAAG GATGTAGGAA GAACAGCAGC CAATAAAATA 
AAAGAACAAT GTCCGCTGGA AGCAGGGCTG CACAGCAGCA GCAAGGTGAC AATACACGAA 
TGTGATGAAG GGTCAGAGCC TTTGGGATTC TGGGATGCAT TAGGAAGGCG AGATCGAAAG 
GCCTATGATT GCATGTTGCA AGATCCGGGA AAGTTTAATT TCACCCCCCG CCTCTTCAGC 
CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC ACTGAGTACG TTTACCCTTC AAGAGACCCT 
ACTGTCATCA ATTCTATGCC CTTCTTGCAA GAGGATCTTT ACACTGCCCC ACAGCCAGCA 
CTGTTCCTTG TTGACAATCA CCATGAAGTG TACCTGTGGC AAGGCTGGTG GCCTGTGGAA 
AACAAAATCA CTGGATCAGC TCGAATCAGG TGGGCTACAG ACAGGAAATG CGCCATGGAG 
ACCGTGCTCC AGTACTGCAA AGGAAAAAAT GTAAAGAAGC CCCCAAAGTC CTATCTCATT 
CATGCCGGCC TAGAGCCTCT GACCTTCACT AATATGTTCC CAAGTTGGGA ACACAGGGAA 
GACATTGCAG AGATCACAGA AATGGATGCT GACGTTTCTA ATCAGATAAT CCTTGTAGAA 
GATGTGCTGG CCAAGCTGTG TAAAACTGTG TATCCCCTAG CTGATCTCTT AGCCAGGCCT 
TTACCTGAGG GAGTTGATCC TCTGAAGCTT GAAATTTATC TTTCAGATGA GGACTTTGAG 
GTTGCATTAG AGATGACAAG AGAAGAGTAC AATGCGCTGC CATCTTGGAA GCAGGTTAAT 
CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282112.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137598.1 Gallus gallus supervillin (SVIL), transcript variant X24, mRNA.

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CloneID	OGa31985
Clone ID Related Accession (Same CDS sequence)	XM_015282112.1
Accession Version	XM_015282112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACCTTGC AGGCATCAGC ACACCAAAAA 
ATACTAGCTA AAGAGGAGAT AAGAGCAGCC AAAGAGGCGA TGGGGCAAGA TCAATCCGAT 
GAACCAGATT CTTCCACATT AAGCTTGGCA GAAAAGATGG CTTTGTTTAA CAAACTGTCT 
CAGCCAGTAT CCAGGGCTAT ATCCACAAGG AGTCGGGGAG ATATTAGGCA CAGAAGGATG 
AATACTCGTT ACCAGACCCA GCCAGTCACT CTTGGAGAAG TTGAGCAGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGAGGAT AGCAGATAAT 
CAGGAGAGTC AAATGACTAT AGAAGAAAGA AAACACCTCA TCACAGCTCG AGAAGAAGCC 
TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT GACTCCACGC AGTTCACCGT AGCTGGTCGA 
ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT GCACTAACAC CAGTAGCATC CCCTTATGGT 
AACAGGCAGA AGTCTACAAC TCCGGTCACA AAGCCCTTGG AAGAAATTGA AGCTAGGCCT 
GATATGCAAT TGGAATCAGA TATGAAGCTT GACAAGCTGG AGTCATTCTT GGGAAGGCTG 
AATAACAAAG TCGCTGGAAT GCAAGAGACA GTGTTGACAG TTACAGGAAA ATCTGTGAAA 
GAGGTGATGA AGCTGGATGA TGATGAAACA TTTTCCAAAT TTTACCGCCG TGTGGATTTC 
CCTTCCTCCT CTGTGCCTTT GGACCTTGAT GAGGACTTTG ATGCCATCTT TGACCCTTAT 
GCACCAAAGT TAATATCCTC TGTAGCAGAG CACAAACGTG CAGTGAGGCC TATGCGCAGG 
GTTCAGTCCT CCAGAAACCC CCTGAAAATG CTGGCAGCAA GAGAAGATAT TCTTCATGAG 
TACACTGAAC AAAGACTGAA TGTTGCCTTC ATGGAGTCAA AGCGAATGAA AGTAGAAAAA 
ATGTCTGCAA ACTCAAATTT CTCAGAAGTA GCACTTGCTG GTTTGGCAAG CAAGGAGAAC 
TTCAGCAACG TTAGTCTGCG GAGTGTTAAC CTAACAGAGC AAAATTCTAA CAACAGTGCT 
GTGCCATACA AGAAGTTAAT GCTGCTGCAA ATTAAAGGAA GAAGACATGT TCAAACAAGA 
TTAGTTGAAC CAAGGGCCTC ATCACTGAAC AGTGGAGACT GTTTCCTATT GTTAACTCCA 
CATCTCTGTT TCTTATGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAAGC AAAGGCTTCA 
GAACTTGCAA CTTTGATTCA GACAAAGAGG GAACTTGGCT GTAGAGCTTC TTATATACAG 
ACTATAGAAG AAGGAATAAA CACCCATACC CATGCAGCCA AAGACTTCTG GAAACTCCTT 
GGTGGCCAAG CAAATTACCA GTCTGCTGGA AGACCTGAAG AGGATGAAAT GTATGAAGCT 
GCCATAATAG AAACTAATTG CATTTACCGT CTTGTAGAGG ATAAACTCAT TCCTGAAGAT 
GACTACTGGG GAAAGATGCC AAAATGTACC CTGCTACAAC CAAAAGAGGT GCTCGTATTT 
GATTTTGGCA GTGAAGTGTA TGTCTGGCAT GGAAAAGAAG TTACTTTAGC ACAAAGAAAA 
GTGGCATTCC AGCTGGCAAA ACACTTATGG AATGGCACCT TCGACTACTC AAATTGTGAC 
ATAAATCCTC TGGACCCAGG AGAATGCAAT CCCCTCATAC CCAGAAAGGG ACAAGGACGT 
CCAGACTGGG CTGTGTTTGG CAGGCTCACA GAACATAATG AGACCATACT GTTCAAAGAA 
AAATTCCTTG ATTGGACAGA GCTGAAGAAA CTCAATGAGA AGAATTCTAG TGAATCCCTT 
CACCAGAAGG AAGAATCCAA ATCTGATTCT AAACCATATG ATGTCATGCT AATGGTACCT 
GTGCCCCAAA CTGCTGTAGG CACGGTTCTG GATGGAATGA ATATTGGCCG GGGCTATGGA 
CTTGTAGAAG GAGAAGATGG GAGGCAGTTT GAAATTATCA CTGCATCTGT GGATGTCTGG 
CACATCCTGG AGTTTGATTA TAGTAGATTG CCTAAACAAA GCATAGGACA GTTCCATGAG 
GGAGACACAT ACGTGGTGAA GTGGAAGTAC ATGGTGAGCA CTACAGTTGG AAGCAGACAA 
AAAGGAGAGC AACAAGTAAG AGCTGTTGGA AAAGAGAAAT GTGTGTATTT CTTTTGGCAA 
GGAAGGCACT CCACAGTGAG TGAGAAAGGG ACATCGGCAC TGATGACAGT GGAACTTGAT 
GAGGAGAGAG GAGCACAGGT ACAAGTGCTT CAAGGGAAAG AACTACCATG CTTCCTGCAG 
TGCTTCCAAG GAGGGATGAT TGTGCATGCT GGAAGAAGGG AAGAGGAAGA AGAAAATGCA 
CAAAGTGACT GGAGACTATA TTGCGTGCGA GGAGAAGTTC CCAATGAAGG AAACTTACTT 
GAAGTAGCAT GTCACTGCAG CAGCCTGCGT TCCCGGACAT CCATGATTGT CCTCAATATA 
AATAAAGCTC TTATCTACCT GTGGCATGGC TGCAAAGCAC AATCTCACAC CAAGGATGTA 
GGAAGAACAG CAGCCAATAA AATAAAAGAA CAATGTCCGC TGGAAGCAGG GCTGCACAGC 
AGCAGCAAGG TGACAATACA CGAATGTGAT GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT 
GCATTAGGAA GGCGAGATCG AAAGGCCTAT GATTGCATGT TGCAAGATCC GGGAAAGTTT 
AATTTCACCC CCCGCCTCTT CAGCCTCAGT AGTTCTTCGG GAGAATTCTC AGCCACTGAG 
TACGTTTACC CTTCAAGAGA CCCTACTGTC ATCAATTCTA TGCCCTTCTT GCAAGAGGAT 
CTTTACACTG CCCCACAGCC AGCACTGTTC CTTGTTGACA ATCACCATGA AGTGTACCTG 
TGGCAAGGCT GGTGGCCTGT GGAAAACAAA ATCACTGGAT CAGCTCGAAT CAGGTGGGCT 
ACAGACAGGA AATGCGCCAT GGAGACCGTG CTCCAGTACT GCAAAGGAAA AAATGTAAAG 
AAGCCCCCAA AGTCCTATCT CATTCATGCC GGCCTAGAGC CTCTGACCTT CACTAATATG 
TTCCCAAGTT GGGAACACAG GGAAGACATT GCAGAGATCA CAGAAATGGA TGCTGACGTT 
TCTAATCAGA TAATCCTTGT AGAAGATGTG CTGGCCAAGC TGTGTAAAAC TGTGTATCCC 
CTAGCTGATC TCTTAGCCAG GCCTTTACCT GAGGGAGTTG ATCCTCTGAA GCTTGAAATT 
TATCTTTCAG ATGAGGACTT TGAGGTTGCA TTAGAGATGA CAAGAGAAGA GTACAATGCG 
CTGCCATCTT GGAAGCAGGT TAATCTGAAA AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137598.1
CDS	16..6726
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282112.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282112.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTGC AGGGAAAGCA 
AAACCTGAAT GGTTCCTTCA GAAAGATTCA GAAGGGGACA CACCATCTCT TATCAGCTGG 
CCCTCCAGAG TAAAAGTTAG AGAGAAACTG GTGAAAGAGG AAAGTGCACG TGGAAGCCCT 
GAACCTGTCA CAGACAGTGT ATCTCAGAGA AAATCCCAGC CAGTGTCAGC CCACTACATG 
CCTCTTCAGA CAGAAACGTC TGCCTTTGAT AGGGTTCTCA ATCAGCCCGT CAGTGCAGTC 
CGCCAACCAA TACATGGGTA TATTCAGCCT GCTGGTGTTG CACACACGGC TCAGCTGATG 
GCAACAGAAG AACCAGAAAG CATCTTCGTT GGCAGCCGCC TCCTTCCAGA CAGTGTCAGC 
CCCTTAACGA AATCAGCAGC AGCCACTGCA CCAGAGGGTG AAAAGAAAGA GACGCTGGCT 
TATGGAGCAA AGCCTGGCGA AGAAGAATCC CCAGAGGGTG AGAAGGCTTC CAAAGGCAGA 
AGACCAATTT TGCCATCTGA GATTCGCAAG CAAGGGAAGA GCCACGAAGG CCTCTTTGGA 
GGAGAAGAAC CAGATACCAG TGTGGGGAGC TCCTCATCTG CAAGCAAGCC GAAGGTTGGC 
TCAGATGTTC AGCAGGAGCA GGAATGCAAA AAGAAGCAGG CGCCTGAGCA GCAGCTGAAG 
ACACCTGAGA ACATGGAGAG GAGCGAAGCT GTCATGTACA TACAGAGCGG GTCTGTATCA 
AAGCCAGCCC AGGCAAAGGG CCCCGTTTGG AAGGTGTCAA AGCATAAAGT CCTGACTCGC 
TCCATGTCTG ACTATACTGT GCCTCCTAAG CTAGAAGCTT TTCATAGTAA GAGCATGGAA 
GCAAACGCAG CAAATGGTGA GATTGGCATG GTTGACACAA AGGTCTCTGT TGCCCAGCTC 
CGTAATGTCT TTCTGGAAAC TGCTCATGCC AACAAGAAAA CGGACCTCGA ATCTCGGTTT 
GAGATGTCAA CGTCAGGCAG CGCTTTGTCT GCCGGTGGTG ACCGTGAAAG AGGGCCGCGA 
AGGCCGAGGC GCTATTTCAC TCCTGGTGAA AATAGAAAGA CTTCTGAGCG ATTTAAAACC 
CAACCTATAA CGTCAGCAGA ACGAAAGGAG ACGGATAGGT CTATTATGTG TGCTGAAATA 
CCAACTGCTG ACGATGAAGA AAAATTAGAT GACCGAGCCA AACTAAGTGT AGCTGCAAAG 
AGACTGCTTT TTAGAGAAAT GGAAAAATCT TTTGAAATGA AGAATATTCC TAAACCACCT 
ACGAGAAGCT CAGCAGTAGA GAGAAGAATG CGGCGTTTGC AGGACAGATC ACACACGCAG 
CCTATTACCA GTGAGGAAGT GGTCATTGCA GCTACCTTGC AGGCATCAGC ACACCAAAAA 
ATACTAGCTA AAGAGGAGAT AAGAGCAGCC AAAGAGGCGA TGGGGCAAGA TCAATCCGAT 
GAACCAGATT CTTCCACATT AAGCTTGGCA GAAAAGATGG CTTTGTTTAA CAAACTGTCT 
CAGCCAGTAT CCAGGGCTAT ATCCACAAGG AGTCGGGGAG ATATTAGGCA CAGAAGGATG 
AATACTCGTT ACCAGACCCA GCCAGTCACT CTTGGAGAAG TTGAGCAGGT GCAAAACGAA 
AGTGGAAAAC TAACTCCCCT GTCAAGTACA GTTTCAACAT CAGTTTCAGC AATGGCTTCT 
GCCCTTTCTG CACTGTTTGC TGGAGACATG CATGCGAAGT CACGTAGTGA TGGAAGTGTG 
AGCGCTGCCA CAAAAGATGA GTTGAGATTC CATGCTTCAG CAGAAAGCTC AGACTCATCA 
GGAAAACGCA CACAGAAGTC AGAACAGTGG CAGCCCTCAA TGGAAGTGCT GGAAAGCAAA 
AGAGCATCAA AGAAGCATGA AGAAGAAGGA AAGAGGTTTC TAGCACATGA GGCTAATGAA 
ATCAGAAAGT ACAGCTCCTT TGAGGATGAA ACCACACATC CTGTTCTTGA AAAAACAGGA 
GACTACCATA AAGAGGCAGC CTACAGCTTC CTGAGGAAGG GCAGCATGGA ACTGTTTAGT 
TCACAACCAC TTTTTCAGCC TCTTGAAAGG AAGGAAATTG ATACCATCAT GCAAGACCAC 
GTGGAGCCTA CCTCTGAACT CACCAGCACA CCAGCAACGA GGACACTTTC CCAGACTACA 
GCTGCAGCAT CCTGTAGGCT ACAGGAGCTG TCTGAACAAC TGGAGGGAAA GTTCTACAAG 
AATTCCTGTG AGATGTTTTC TGCTGGAGAG AACAAAATAC AGACAACTGA GGATGCCACC 
GATAGCTCCA GCAAAACGAT GTCAATTAAA GAAAGGTTGG CATTGCTGAA GAAAAGTGGT 
GAAGAAGATT GGAAGAGCAG GCTCAGTAAA AAGCAGGAAT ATGCAAAAGT GTCTGCCACT 
GATCGTAGCG CACAGATGCA AGAAGTTGAG CAGCTGTTGA AAAAGAGGAT AGCAGATAAT 
CAGGAGAGTC AAATGACTAT AGAAGAAAGA AAACACCTCA TCACAGCTCG AGAAGAAGCC 
TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT GACTCCACGC AGTTCACCGT AGCTGGTCGA 
ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT GCACTAACAC CAGTAGCATC CCCTTATGGT 
AACAGGCAGA AGTCTACAAC TCCGGTCACA AAGCCCTTGG AAGAAATTGA AGCTAGGCCT 
GATATGCAAT TGGAATCAGA TATGAAGCTT GACAAGCTGG AGTCATTCTT GGGAAGGCTG 
AATAACAAAG TCGCTGGAAT GCAAGAGACA GTGTTGACAG TTACAGGAAA ATCTGTGAAA 
GAGGTGATGA AGCTGGATGA TGATGAAACA TTTTCCAAAT TTTACCGCCG TGTGGATTTC 
CCTTCCTCCT CTGTGCCTTT GGACCTTGAT GAGGACTTTG ATGCCATCTT TGACCCTTAT 
GCACCAAAGT TAATATCCTC TGTAGCAGAG CACAAACGTG CAGTGAGGCC TATGCGCAGG 
GTTCAGTCCT CCAGAAACCC CCTGAAAATG CTGGCAGCAA GAGAAGATAT TCTTCATGAG 
TACACTGAAC AAAGACTGAA TGTTGCCTTC ATGGAGTCAA AGCGAATGAA AGTAGAAAAA 
ATGTCTGCAA ACTCAAATTT CTCAGAAGTA GCACTTGCTG GTTTGGCAAG CAAGGAGAAC 
TTCAGCAACG TTAGTCTGCG GAGTGTTAAC CTAACAGAGC AAAATTCTAA CAACAGTGCT 
GTGCCATACA AGAAGTTAAT GCTGCTGCAA ATTAAAGGAA GAAGACATGT TCAAACAAGA 
TTAGTTGAAC CAAGGGCCTC ATCACTGAAC AGTGGAGACT GTTTCCTATT GTTAACTCCA 
CATCTCTGTT TCTTATGGGT AGGAGAGTTT GCAAATGTCA TAGAAAAAGC AAAGGCTTCA 
GAACTTGCAA CTTTGATTCA GACAAAGAGG GAACTTGGCT GTAGAGCTTC TTATATACAG 
ACTATAGAAG AAGGAATAAA CACCCATACC CATGCAGCCA AAGACTTCTG GAAACTCCTT 
GGTGGCCAAG CAAATTACCA GTCTGCTGGA AGACCTGAAG AGGATGAAAT GTATGAAGCT 
GCCATAATAG AAACTAATTG CATTTACCGT CTTGTAGAGG ATAAACTCAT TCCTGAAGAT 
GACTACTGGG GAAAGATGCC AAAATGTACC CTGCTACAAC CAAAAGAGGT GCTCGTATTT 
GATTTTGGCA GTGAAGTGTA TGTCTGGCAT GGAAAAGAAG TTACTTTAGC ACAAAGAAAA 
GTGGCATTCC AGCTGGCAAA ACACTTATGG AATGGCACCT TCGACTACTC AAATTGTGAC 
ATAAATCCTC TGGACCCAGG AGAATGCAAT CCCCTCATAC CCAGAAAGGG ACAAGGACGT 
CCAGACTGGG CTGTGTTTGG CAGGCTCACA GAACATAATG AGACCATACT GTTCAAAGAA 
AAATTCCTTG ATTGGACAGA GCTGAAGAAA CTCAATGAGA AGAATTCTAG TGAATCCCTT 
CACCAGAAGG AAGAATCCAA ATCTGATTCT AAACCATATG ATGTCATGCT AATGGTACCT 
GTGCCCCAAA CTGCTGTAGG CACGGTTCTG GATGGAATGA ATATTGGCCG GGGCTATGGA 
CTTGTAGAAG GAGAAGATGG GAGGCAGTTT GAAATTATCA CTGCATCTGT GGATGTCTGG 
CACATCCTGG AGTTTGATTA TAGTAGATTG CCTAAACAAA GCATAGGACA GTTCCATGAG 
GGAGACACAT ACGTGGTGAA GTGGAAGTAC ATGGTGAGCA CTACAGTTGG AAGCAGACAA 
AAAGGAGAGC AACAAGTAAG AGCTGTTGGA AAAGAGAAAT GTGTGTATTT CTTTTGGCAA 
GGAAGGCACT CCACAGTGAG TGAGAAAGGG ACATCGGCAC TGATGACAGT GGAACTTGAT 
GAGGAGAGAG GAGCACAGGT ACAAGTGCTT CAAGGGAAAG AACTACCATG CTTCCTGCAG 
TGCTTCCAAG GAGGGATGAT TGTGCATGCT GGAAGAAGGG AAGAGGAAGA AGAAAATGCA 
CAAAGTGACT GGAGACTATA TTGCGTGCGA GGAGAAGTTC CCAATGAAGG AAACTTACTT 
GAAGTAGCAT GTCACTGCAG CAGCCTGCGT TCCCGGACAT CCATGATTGT CCTCAATATA 
AATAAAGCTC TTATCTACCT GTGGCATGGC TGCAAAGCAC AATCTCACAC CAAGGATGTA 
GGAAGAACAG CAGCCAATAA AATAAAAGAA CAATGTCCGC TGGAAGCAGG GCTGCACAGC 
AGCAGCAAGG TGACAATACA CGAATGTGAT GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT 
GCATTAGGAA GGCGAGATCG AAAGGCCTAT GATTGCATGT TGCAAGATCC GGGAAAGTTT 
AATTTCACCC CCCGCCTCTT CAGCCTCAGT AGTTCTTCGG GAGAATTCTC AGCCACTGAG 
TACGTTTACC CTTCAAGAGA CCCTACTGTC ATCAATTCTA TGCCCTTCTT GCAAGAGGAT 
CTTTACACTG CCCCACAGCC AGCACTGTTC CTTGTTGACA ATCACCATGA AGTGTACCTG 
TGGCAAGGCT GGTGGCCTGT GGAAAACAAA ATCACTGGAT CAGCTCGAAT CAGGTGGGCT 
ACAGACAGGA AATGCGCCAT GGAGACCGTG CTCCAGTACT GCAAAGGAAA AAATGTAAAG 
AAGCCCCCAA AGTCCTATCT CATTCATGCC GGCCTAGAGC CTCTGACCTT CACTAATATG 
TTCCCAAGTT GGGAACACAG GGAAGACATT GCAGAGATCA CAGAAATGGA TGCTGACGTT 
TCTAATCAGA TAATCCTTGT AGAAGATGTG CTGGCCAAGC TGTGTAAAAC TGTGTATCCC 
CTAGCTGATC TCTTAGCCAG GCCTTTACCT GAGGGAGTTG ATCCTCTGAA GCTTGAAATT 
TATCTTTCAG ATGAGGACTT TGAGGTTGCA TTAGAGATGA CAAGAGAAGA GTACAATGCG 
CTGCCATCTT GGAAGCAGGT TAATCTGAAA AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_015282113.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137599.1 Gallus gallus supervillin (SVIL), transcript variant X25, mRNA.

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CloneID	OGa31986
Clone ID Related Accession (Same CDS sequence)	XM_015282113.1
Accession Version	XM_015282113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACCT TGCAGGCATC AGCACACCAA 
AAAATACTAG CTAAAGAGGA GATAAGAGCA GCCAAAGAGG CGATGGGGCA AGATCAATCC 
GATGAACCAG ATTCTTCCAC ATTAAGCTTG GCAGAAAAGA TGGCTTTGTT TAACAAACTG 
TCTCAGCCAG TATCCAGGGC TATATCCACA AGGAGTCGGG GAGATATTAG GCACAGAAGG 
ATGAATACTC GTTACCAGAC CCAGCCAGTC ACTCTTGGAG AAGTTGAGCA GATGCAATGG 
AAAAAGAGTA AACCGGTGCA AAACGAAAGT GGAAAACTAA CTCCCCTGTC AAGTACAGTT 
TCAACATCAG TTTCAGCAAT GGCTTCTGCC CTTTCTGCAC TGTTTGCTGG AGACATGCAT 
GCGAAGTCAC GTAGTGATGG AAGTGTGAGC GCTGCCACAA AAGATGAGTT GAGATTCCAT 
GCTTCAGCAG AAAGCTCAGA CTCATCAGGA AAACGCACAC AGAAGTCAGA ACAGTGGCAG 
CCCTCAATGG AAGTGCTGGA AAGCAAAAGA GCATCAAAGA AGCATGAAGA AGAAGGAAAG 
AGGTTTCTAG CACATGAGGC TAATGAAATC AGAAAGTACA GCTCCTTTGA GGATGAAACC 
ACACATCCTG TTCTTGAAAA AACAGGAGAC TACCATAAAG AGGCAGCCTA CAGCTTCCTG 
AGGAAGGGCA GCATGGAACT GTTTAGTTCA CAACCACTTT TTCAGCCTCT TGAAAGGAAG 
GAAATTGATA CCATCATGCA AGACCACGTG GAGCCTACCT CTGAACTCAC CAGCACACCA 
GCAACGAGGA CACTTTCCCA GACTACAGCT GCAGCATCCT GTAGGCTACA GGAGCTGTCT 
GAACAACTGG AGGGAAAGTT CTACAAGAAT TCCTGTGAGA TGTTTTCTGC TGGAGAGAAC 
AAAATACAGA CAACTGAGGA TGCCACCGAT AGCTCCAGCA AAACGATGTC AATTAAAGAA 
AGGTTGGCAT TGCTGAAGAA AAGTGGTGAA GAAGATTGGA AGAGCAGGCT CAGTAAAAAG 
CAGGAATATG CAAAAGTGTC TGCCACTGAT CGTAGCGCAC AGATGCAAGA AGTTGAGCAG 
CTGTTGAAAA AGCAGAGGAT AGCAGATAAT CAGGAGAGTC AAATGACTAT AGAAGAAAGA 
AAACACCTCA TCACAGCTCG AGAAGAAGCC TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT 
GACTCCACGC AGTTCACCGT AGCTGGTCGA ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT 
GCACTAACAC CAGTAGCATC CCCTTATGGT AACAGGCAGA AGTCTACAAC TCCGGTCACA 
AAGCCCTTGG AAGAAATTGA AGCTAGGCCT GATATGCAAT TGGAATCAGA TATGAAGCTT 
GACAAGCTGG AGTCATTCTT GGGAAGGCTG AATAACAAAG TCGCTGGAAT GCAAGAGACA 
GTGTTGACAG TTACAGGAAA ATCTGTGAAA GAGGTGATGA AGCTGGATGA TGATGAAACA 
TTTTCCAAAT TTTACCGCCG TGTGGATTTC CCTTCCTCCT CTGTGCCTTT GGACCTTGAT 
GAGGACTTTG ATGCCATCTT TGACCCTTAT GCACCAAAGT TAATATCCTC TGTAGCAGAG 
CACAAACGTG CAGTGAGGCC TATGCGCAGG GTTCAGTCCT CCAGAAACCC CCTGAAAATG 
CTGGCAGCAA GAGAAGATAT TCTTCATGAG TACACTGAAC AAAGACTGAA TGTTGCCTTC 
ATGGAGTCAA AGCGAATGAA AGTAGAAAAA ATGTCTGCAA ACTCAAATTT CTCAGAAGTA 
GCACTTGCTG GTTTGGCAAG CAAGGAGAAC TTCAGCAACG TTAGTCTGCG GAGTGTTAAC 
CTAACAGAGC AAAATTCTAA CAACAGTGCT GTGCCATACA AGAAGTTAAT GCTGCTGCAA 
ATTAAAGGAA GAAGACATGT TCAAACAAGA TTAGTTGAAC CAAGGGCCTC ATCACTGAAC 
AGTGGAGACT GTTTCCTATT GTTAACTCCA CATCTCTGTT TCTTATGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAAGC AAAGGCTTCA GAACTTGCAA CTTTGATTCA GACAAAGAGG 
GAACTTGGCT GTAGAGCTTC TTATATACAG ACTATAGAAG AAGGAATAAA CACCCATACC 
CATGCAGCCA AAGACTTCTG GAAACTCCTT GGTGGCCAAG CAAATTACCA GTCTGCTGGA 
AGACCTGAAG AGGATGAAAT GTATGAAGCT GCCATAATAG AAACTAATTG CATTTACCGT 
CTTGTAGAGG ATAAACTCAT TCCTGAAGAT GACTACTGGG GAAAGATGCC AAAATGTACC 
CTGCTACAAC CAAAAGAGGT GCTCGTATTT GATTTTGGCA GTGAAGTGTA TGTCTGGCAT 
GGAAAAGAAG TTACTTTAGC ACAAAGAAAA GTGGCATTCC AGCTGGCAAA ACACTTATGG 
AATGGCACCT TCGACTACTC AAATTGTGAC ATAAATCCTC TGGACCCAGG AGAATGCAAT 
CCCCTCATAC CCAGAAAGGG ACAAGGACGT CCAGACTGGG CTGTGTTTGG CAGGCTCACA 
GAACATAATG AGACCATACT GTTCAAAGAA AAATTCCTTG ATTGGACAGA GCTGAAGAAA 
CTCAATGAGA AGAATTCTAG TGAATCCCTT CACCAGAAGG AAGAATCCAA ATCTGATTCT 
AAACCATATG ATGTCATGCT AATGGTACCT GTGCCCCAAA CTGCTGTAGG CACGGTTCTG 
GATGGAATGA ATATTGGCCG GGGCTATGGA CTTGTAGAAG GAGAAGATGG GAGGCAGTTT 
GAAATTATCA CTGCATCTGT GGATGTCTGG CACATCCTGG AGTTTGATTA TAGTAGATTG 
CCTAAACAAA GCATAGGACA GTTCCATGAG GGAGACACAT ACGTGGTGAA GTGGAAGTAC 
ATGGTGAGCA CTACAGTTGG AAGCAGACAA AAAGGAGAGC AACAAGTAAG AGCTGTTGGA 
AAAGAGAAAT GTGTGTATTT CTTTTGGCAA GGAAGGCACT CCACAGTGAG TGAGAAAGGG 
ACATCGGCAC TGATGACAGT GGAACTTGAT GAGGAGAGAG GAGCACAGGT ACAAGTGCTT 
CAAGGGAAAG AACTACCATG CTTCCTGCAG TGCTTCCAAG GAGGGATGAT TGTGCATGCT 
GGAAGAAGGG AAGAGGAAGA AGAAAATGCA CAAAGTGACT GGAGACTATA TTGCGTGCGA 
GGAGAAGTTC CCAATGAAGG AAACTTACTT GAAGTAGCAT GTCACTGCAG CAGCCTGCGT 
TCCCGGACAT CCATGATTGT CCTCAATATA AATAAAGCTC TTATCTACCT GTGGCATGGC 
TGCAAAGCAC AATCTCACAC CAAGGATGTA GGAAGAACAG CAGCCAATAA AATAAAAGAA 
CAATGTCCGC TGGAAGCAGG GCTGCACAGC AGCAGCAAGG TGACAATACA CGAATGTGAT 
GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT GCATTAGGAA GGCGAGATCG AAAGGCCTAT 
GATTGCATGT TGCAAGATCC GGGAAAGTTT AATTTCACCC CCCGCCTCTT CAGCCTCAGT 
AGTTCTTCGG GAGAATTCTC AGCCACTGAG TACGTTTACC CTTCAAGAGA CCCTACTGTC 
ATCAATTCTA TGCCCTTCTT GCAAGAGGAT CTTTACACTG CCCCACAGCC AGCACTGTTC 
CTTGTTGACA ATCACCATGA AGTGTACCTG TGGCAAGGCT GGTGGCCTGT GGAAAACAAA 
ATCACTGGAT CAGCTCGAAT CAGGTGGGCT ACAGACAGGA AATGCGCCAT GGAGACCGTG 
CTCCAGTACT GCAAAGGAAA AAATGTAAAG AAGCCCCCAA AGTCCTATCT CATTCATGCC 
GGCCTAGAGC CTCTGACCTT CACTAATATG TTCCCAAGTT GGGAACACAG GGAAGACATT 
GCAGAGATCA CAGAAATGGA TGCTGACGTT TCTAATCAGA TAATCCTTGT AGAAGATGTG 
CTGGCCAAGC TGTGTAAAAC TGTGTATCCC CTAGCTGATC TCTTAGCCAG GCCTTTACCT 
GAGGGAGTTG ATCCTCTGAA GCTTGAAATT TATCTTTCAG ATGAGGACTT TGAGGTTGCA 
TTAGAGATGA CAAGAGAAGA GTACAATGCG CTGCCATCTT GGAAGCAGGT TAATCTGAAA 
AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137599.1
CDS	17..6757
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_015282113.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015282113.1

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACCT TGCAGGCATC AGCACACCAA 
AAAATACTAG CTAAAGAGGA GATAAGAGCA GCCAAAGAGG CGATGGGGCA AGATCAATCC 
GATGAACCAG ATTCTTCCAC ATTAAGCTTG GCAGAAAAGA TGGCTTTGTT TAACAAACTG 
TCTCAGCCAG TATCCAGGGC TATATCCACA AGGAGTCGGG GAGATATTAG GCACAGAAGG 
ATGAATACTC GTTACCAGAC CCAGCCAGTC ACTCTTGGAG AAGTTGAGCA GATGCAATGG 
AAAAAGAGTA AACCGGTGCA AAACGAAAGT GGAAAACTAA CTCCCCTGTC AAGTACAGTT 
TCAACATCAG TTTCAGCAAT GGCTTCTGCC CTTTCTGCAC TGTTTGCTGG AGACATGCAT 
GCGAAGTCAC GTAGTGATGG AAGTGTGAGC GCTGCCACAA AAGATGAGTT GAGATTCCAT 
GCTTCAGCAG AAAGCTCAGA CTCATCAGGA AAACGCACAC AGAAGTCAGA ACAGTGGCAG 
CCCTCAATGG AAGTGCTGGA AAGCAAAAGA GCATCAAAGA AGCATGAAGA AGAAGGAAAG 
AGGTTTCTAG CACATGAGGC TAATGAAATC AGAAAGTACA GCTCCTTTGA GGATGAAACC 
ACACATCCTG TTCTTGAAAA AACAGGAGAC TACCATAAAG AGGCAGCCTA CAGCTTCCTG 
AGGAAGGGCA GCATGGAACT GTTTAGTTCA CAACCACTTT TTCAGCCTCT TGAAAGGAAG 
GAAATTGATA CCATCATGCA AGACCACGTG GAGCCTACCT CTGAACTCAC CAGCACACCA 
GCAACGAGGA CACTTTCCCA GACTACAGCT GCAGCATCCT GTAGGCTACA GGAGCTGTCT 
GAACAACTGG AGGGAAAGTT CTACAAGAAT TCCTGTGAGA TGTTTTCTGC TGGAGAGAAC 
AAAATACAGA CAACTGAGGA TGCCACCGAT AGCTCCAGCA AAACGATGTC AATTAAAGAA 
AGGTTGGCAT TGCTGAAGAA AAGTGGTGAA GAAGATTGGA AGAGCAGGCT CAGTAAAAAG 
CAGGAATATG CAAAAGTGTC TGCCACTGAT CGTAGCGCAC AGATGCAAGA AGTTGAGCAG 
CTGTTGAAAA AGCAGAGGAT AGCAGATAAT CAGGAGAGTC AAATGACTAT AGAAGAAAGA 
AAACACCTCA TCACAGCTCG AGAAGAAGCC TGGAAGTCCA GAGGTAAAGG CGCAGCAAAT 
GACTCCACGC AGTTCACCGT AGCTGGTCGA ATGGTAAAAA AAGGCCTGGC TTCTCCAAGT 
GCACTAACAC CAGTAGCATC CCCTTATGGT AACAGGCAGA AGTCTACAAC TCCGGTCACA 
AAGCCCTTGG AAGAAATTGA AGCTAGGCCT GATATGCAAT TGGAATCAGA TATGAAGCTT 
GACAAGCTGG AGTCATTCTT GGGAAGGCTG AATAACAAAG TCGCTGGAAT GCAAGAGACA 
GTGTTGACAG TTACAGGAAA ATCTGTGAAA GAGGTGATGA AGCTGGATGA TGATGAAACA 
TTTTCCAAAT TTTACCGCCG TGTGGATTTC CCTTCCTCCT CTGTGCCTTT GGACCTTGAT 
GAGGACTTTG ATGCCATCTT TGACCCTTAT GCACCAAAGT TAATATCCTC TGTAGCAGAG 
CACAAACGTG CAGTGAGGCC TATGCGCAGG GTTCAGTCCT CCAGAAACCC CCTGAAAATG 
CTGGCAGCAA GAGAAGATAT TCTTCATGAG TACACTGAAC AAAGACTGAA TGTTGCCTTC 
ATGGAGTCAA AGCGAATGAA AGTAGAAAAA ATGTCTGCAA ACTCAAATTT CTCAGAAGTA 
GCACTTGCTG GTTTGGCAAG CAAGGAGAAC TTCAGCAACG TTAGTCTGCG GAGTGTTAAC 
CTAACAGAGC AAAATTCTAA CAACAGTGCT GTGCCATACA AGAAGTTAAT GCTGCTGCAA 
ATTAAAGGAA GAAGACATGT TCAAACAAGA TTAGTTGAAC CAAGGGCCTC ATCACTGAAC 
AGTGGAGACT GTTTCCTATT GTTAACTCCA CATCTCTGTT TCTTATGGGT AGGAGAGTTT 
GCAAATGTCA TAGAAAAAGC AAAGGCTTCA GAACTTGCAA CTTTGATTCA GACAAAGAGG 
GAACTTGGCT GTAGAGCTTC TTATATACAG ACTATAGAAG AAGGAATAAA CACCCATACC 
CATGCAGCCA AAGACTTCTG GAAACTCCTT GGTGGCCAAG CAAATTACCA GTCTGCTGGA 
AGACCTGAAG AGGATGAAAT GTATGAAGCT GCCATAATAG AAACTAATTG CATTTACCGT 
CTTGTAGAGG ATAAACTCAT TCCTGAAGAT GACTACTGGG GAAAGATGCC AAAATGTACC 
CTGCTACAAC CAAAAGAGGT GCTCGTATTT GATTTTGGCA GTGAAGTGTA TGTCTGGCAT 
GGAAAAGAAG TTACTTTAGC ACAAAGAAAA GTGGCATTCC AGCTGGCAAA ACACTTATGG 
AATGGCACCT TCGACTACTC AAATTGTGAC ATAAATCCTC TGGACCCAGG AGAATGCAAT 
CCCCTCATAC CCAGAAAGGG ACAAGGACGT CCAGACTGGG CTGTGTTTGG CAGGCTCACA 
GAACATAATG AGACCATACT GTTCAAAGAA AAATTCCTTG ATTGGACAGA GCTGAAGAAA 
CTCAATGAGA AGAATTCTAG TGAATCCCTT CACCAGAAGG AAGAATCCAA ATCTGATTCT 
AAACCATATG ATGTCATGCT AATGGTACCT GTGCCCCAAA CTGCTGTAGG CACGGTTCTG 
GATGGAATGA ATATTGGCCG GGGCTATGGA CTTGTAGAAG GAGAAGATGG GAGGCAGTTT 
GAAATTATCA CTGCATCTGT GGATGTCTGG CACATCCTGG AGTTTGATTA TAGTAGATTG 
CCTAAACAAA GCATAGGACA GTTCCATGAG GGAGACACAT ACGTGGTGAA GTGGAAGTAC 
ATGGTGAGCA CTACAGTTGG AAGCAGACAA AAAGGAGAGC AACAAGTAAG AGCTGTTGGA 
AAAGAGAAAT GTGTGTATTT CTTTTGGCAA GGAAGGCACT CCACAGTGAG TGAGAAAGGG 
ACATCGGCAC TGATGACAGT GGAACTTGAT GAGGAGAGAG GAGCACAGGT ACAAGTGCTT 
CAAGGGAAAG AACTACCATG CTTCCTGCAG TGCTTCCAAG GAGGGATGAT TGTGCATGCT 
GGAAGAAGGG AAGAGGAAGA AGAAAATGCA CAAAGTGACT GGAGACTATA TTGCGTGCGA 
GGAGAAGTTC CCAATGAAGG AAACTTACTT GAAGTAGCAT GTCACTGCAG CAGCCTGCGT 
TCCCGGACAT CCATGATTGT CCTCAATATA AATAAAGCTC TTATCTACCT GTGGCATGGC 
TGCAAAGCAC AATCTCACAC CAAGGATGTA GGAAGAACAG CAGCCAATAA AATAAAAGAA 
CAATGTCCGC TGGAAGCAGG GCTGCACAGC AGCAGCAAGG TGACAATACA CGAATGTGAT 
GAAGGGTCAG AGCCTTTGGG ATTCTGGGAT GCATTAGGAA GGCGAGATCG AAAGGCCTAT 
GATTGCATGT TGCAAGATCC GGGAAAGTTT AATTTCACCC CCCGCCTCTT CAGCCTCAGT 
AGTTCTTCGG GAGAATTCTC AGCCACTGAG TACGTTTACC CTTCAAGAGA CCCTACTGTC 
ATCAATTCTA TGCCCTTCTT GCAAGAGGAT CTTTACACTG CCCCACAGCC AGCACTGTTC 
CTTGTTGACA ATCACCATGA AGTGTACCTG TGGCAAGGCT GGTGGCCTGT GGAAAACAAA 
ATCACTGGAT CAGCTCGAAT CAGGTGGGCT ACAGACAGGA AATGCGCCAT GGAGACCGTG 
CTCCAGTACT GCAAAGGAAA AAATGTAAAG AAGCCCCCAA AGTCCTATCT CATTCATGCC 
GGCCTAGAGC CTCTGACCTT CACTAATATG TTCCCAAGTT GGGAACACAG GGAAGACATT 
GCAGAGATCA CAGAAATGGA TGCTGACGTT TCTAATCAGA TAATCCTTGT AGAAGATGTG 
CTGGCCAAGC TGTGTAAAAC TGTGTATCCC CTAGCTGATC TCTTAGCCAG GCCTTTACCT 
GAGGGAGTTG ATCCTCTGAA GCTTGAAATT TATCTTTCAG ATGAGGACTT TGAGGTTGCA 
TTAGAGATGA CAAGAGAAGA GTACAATGCG CTGCCATCTT GGAAGCAGGT TAATCTGAAA 
AAGGCAAAAG GACTTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_004939197.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939254.1 Gallus gallus supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OGa04062
Clone ID Related Accession (Same CDS sequence)	XM_004939197.2
Accession Version	XM_004939197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939254.1
CDS	18..6932
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKEPVYASTYSPVIPAVHKFHPFLPINQQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_004939197.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939197.2

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGGA ACCTGTATAT 
GCTTCTACTT ACTCTCCTGT TATACCTGCT GTCCATAAAT TTCATCCTTT TCTACCTATT 
AATCAGCAGA GGATAGCAGA TAATCAGGAG AGTCAAATGA CTATAGAAGA AAGAAAACAC 
CTCATCACAG CTCGAGAAGA AGCCTGGAAG TCCAGAGGTA AAGGCGCAGC AAATGACTCC 
ACGCAGTTCA CCGTAGCTGG TCGAATGGTA AAAAAAGGCC TGGCTTCTCC AAGTGCACTA 
ACACCAGTAG CATCCCCTTA TGGTAACAGG CAGAAGTCTA CAACTCCGGT CACAAAGCCC 
TTGGAAGAAA TTGAAGCTAG GCCTGATATG CAATTGGAAT CAGATATGAA GCTTGACAAG 
CTGGAGTCAT TCTTGGGAAG GCTGAATAAC AAAGTCGCTG GAATGCAAGA GACAGTGTTG 
ACAGTTACAG GAAAATCTGT GAAAGAGGTG ATGAAGCTGG ATGATGATGA AACATTTTCC 
AAATTTTACC GCCGTGTGGA TTTCCCTTCC TCCTCTGTGC CTTTGGACCT TGATGAGGAC 
TTTGATGCCA TCTTTGACCC TTATGCACCA AAGTTAATAT CCTCTGTAGC AGAGCACAAA 
CGTGCAGTGA GGCCTATGCG CAGGGTTCAG TCCTCCAGAA ACCCCCTGAA AATGCTGGCA 
GCAAGAGAAG ATATTCTTCA TGAGTACACT GAACAAAGAC TGAATGTTGC CTTCATGGAG 
TCAAAGCGAA TGAAAGTAGA AAAAATGTCT GCAAACTCAA ATTTCTCAGA AGTAGCACTT 
GCTGGTTTGG CAAGCAAGGA GAACTTCAGC AACGTTAGTC TGCGGAGTGT TAACCTAACA 
GAGCAAAATT CTAACAACAG TGCTGTGCCA TACAAGAAGT TAATGCTGCT GCAAATTAAA 
GGAAGAAGAC ATGTTCAAAC AAGATTAGTT GAACCAAGGG CCTCATCACT GAACAGTGGA 
GACTGTTTCC TATTGTTAAC TCCACATCTC TGTTTCTTAT GGGTAGGAGA GTTTGCAAAT 
GTCATAGAAA AAGCAAAGGC TTCAGAACTT GCAACTTTGA TTCAGACAAA GAGGGAACTT 
GGCTGTAGAG CTTCTTATAT ACAGACTATA GAAGAAGGAA TAAACACCCA TACCCATGCA 
GCCAAAGACT TCTGGAAACT CCTTGGTGGC CAAGCAAATT ACCAGTCTGC TGGAAGACCT 
GAAGAGGATG AAATGTATGA AGCTGCCATA ATAGAAACTA ATTGCATTTA CCGTCTTGTA 
GAGGATAAAC TCATTCCTGA AGATGACTAC TGGGGAAAGA TGCCAAAATG TACCCTGCTA 
CAACCAAAAG AGGTGCTCGT ATTTGATTTT GGCAGTGAAG TGTATGTCTG GCATGGAAAA 
GAAGTTACTT TAGCACAAAG AAAAGTGGCA TTCCAGCTGG CAAAACACTT ATGGAATGGC 
ACCTTCGACT ACTCAAATTG TGACATAAAT CCTCTGGACC CAGGAGAATG CAATCCCCTC 
ATACCCAGAA AGGGACAAGG ACGTCCAGAC TGGGCTGTGT TTGGCAGGCT CACAGAACAT 
AATGAGACCA TACTGTTCAA AGAAAAATTC CTTGATTGGA CAGAGCTGAA GAAACTCAAT 
GAGAAGAATT CTAGTGAATC CCTTCACCAG AAGGAAGAAT CCAAATCTGA TTCTAAACCA 
TATGATGTCA TGCTAATGGT ACCTGTGCCC CAAACTGCTG TAGGCACGGT TCTGGATGGA 
ATGAATATTG GCCGGGGCTA TGGACTTGTA GAAGGAGAAG ATGGGAGGCA GTTTGAAATT 
ATCACTGCAT CTGTGGATGT CTGGCACATC CTGGAGTTTG ATTATAGTAG ATTGCCTAAA 
CAAAGCATAG GACAGTTCCA TGAGGGAGAC ACATACGTGG TGAAGTGGAA GTACATGGTG 
AGCACTACAG TTGGAAGCAG ACAAAAAGGA GAGCAACAAG TAAGAGCTGT TGGAAAAGAG 
AAATGTGTGT ATTTCTTTTG GCAAGGAAGG CACTCCACAG TGAGTGAGAA AGGGACATCG 
GCACTGATGA CAGTGGAACT TGATGAGGAG AGAGGAGCAC AGGTACAAGT GCTTCAAGGG 
AAAGAACTAC CATGCTTCCT GCAGTGCTTC CAAGGAGGGA TGATTGTGCA TGCTGGAAGA 
AGGGAAGAGG AAGAAGAAAA TGCACAAAGT GACTGGAGAC TATATTGCGT GCGAGGAGAA 
GTTCCCAATG AAGGAAACTT ACTTGAAGTA GCATGTCACT GCAGCAGCCT GCGTTCCCGG 
ACATCCATGA TTGTCCTCAA TATAAATAAA GCTCTTATCT ACCTGTGGCA TGGCTGCAAA 
GCACAATCTC ACACCAAGGA TGTAGGAAGA ACAGCAGCCA ATAAAATAAA AGAACAATGT 
CCGCTGGAAG CAGGGCTGCA CAGCAGCAGC AAGGTGACAA TACACGAATG TGATGAAGGG 
TCAGAGCCTT TGGGATTCTG GGATGCATTA GGAAGGCGAG ATCGAAAGGC CTATGATTGC 
ATGTTGCAAG ATCCGGGAAA GTTTAATTTC ACCCCCCGCC TCTTCAGCCT CAGTAGTTCT 
TCGGGAGAAT TCTCAGCCAC TGAGTACGTT TACCCTTCAA GAGACCCTAC TGTCATCAAT 
TCTATGCCCT TCTTGCAAGA GGATCTTTAC ACTGCCCCAC AGCCAGCACT GTTCCTTGTT 
GACAATCACC ATGAAGTGTA CCTGTGGCAA GGCTGGTGGC CTGTGGAAAA CAAAATCACT 
GGATCAGCTC GAATCAGGTG GGCTACAGAC AGGAAATGCG CCATGGAGAC CGTGCTCCAG 
TACTGCAAAG GAAAAAATGT AAAGAAGCCC CCAAAGTCCT ATCTCATTCA TGCCGGCCTA 
GAGCCTCTGA CCTTCACTAA TATGTTCCCA AGTTGGGAAC ACAGGGAAGA CATTGCAGAG 
ATCACAGAAA TGGATGCTGA CGTTTCTAAT CAGATAATCC TTGTAGAAGA TGTGCTGGCC 
AAGCTGTGTA AAACTGTGTA TCCCCTAGCT GATCTCTTAG CCAGGCCTTT ACCTGAGGGA 
GTTGATCCTC TGAAGCTTGA AATTTATCTT TCAGATGAGG ACTTTGAGGT TGCATTAGAG 
ATGACAAGAG AAGAGTACAA TGCGCTGCCA TCTTGGAAGC AGGTTAATCT GAAAAAGGCA 
AAAGGACTTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_004939199.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939256.1 Gallus gallus supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OGa04064
Clone ID Related Accession (Same CDS sequence)	XM_004939199.2 , XM_004939199.3
Accession Version	XM_004939199.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGCA GAGGATAGCA 
GATAATCAGG AGAGTCAAAT GACTATAGAA GAAAGAAAAC ACCTCATCAC AGCTCGAGAA 
GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA GCAAATGACT CCACGCAGTT CACCGTAGCT 
GGTCGAATGG TAAAAAAAGG CCTGGCTTCT CCAAGTGCAC TAACACCAGT AGCATCCCCT 
TATGGTAACA GGCAGAAGTC TACAACTCCG GTCACAAAGC CCTTGGAAGA AATTGAAGCT 
AGGCCTGATA TGCAATTGGA ATCAGATATG AAGCTTGACA AGCTGGAGTC ATTCTTGGGA 
AGGCTGAATA ACAAAGTCGC TGGAATGCAA GAGACAGTGT TGACAGTTAC AGGAAAATCT 
GTGAAAGAGG TGATGAAGCT GGATGATGAT GAAACATTTT CCAAATTTTA CCGCCGTGTG 
GATTTCCCTT CCTCCTCTGT GCCTTTGGAC CTTGATGAGG ACTTTGATGC CATCTTTGAC 
CCTTATGCAC CAAAGTTAAT ATCCTCTGTA GCAGAGCACA AACGTGCAGT GAGGCCTATG 
CGCAGGGTTC AGTCCTCCAG AAACCCCCTG AAAATGCTGG CAGCAAGAGA AGATATTCTT 
CATGAGTACA CTGAACAAAG ACTGAATGTT GCCTTCATGG AGTCAAAGCG AATGAAAGTA 
GAAAAAATGT CTGCAAACTC AAATTTCTCA GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG 
GAGAACTTCA GCAACGTTAG TCTGCGGAGT GTTAACCTAA CAGAGCAAAA TTCTAACAAC 
AGTGCTGTGC CATACAAGAA GTTAATGCTG CTGCAAATTA AAGGAAGAAG ACATGTTCAA 
ACAAGATTAG TTGAACCAAG GGCCTCATCA CTGAACAGTG GAGACTGTTT CCTATTGTTA 
ACTCCACATC TCTGTTTCTT ATGGGTAGGA GAGTTTGCAA ATGTCATAGA AAAAGCAAAG 
GCTTCAGAAC TTGCAACTTT GATTCAGACA AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT 
ATACAGACTA TAGAAGAAGG AATAAACACC CATACCCATG CAGCCAAAGA CTTCTGGAAA 
CTCCTTGGTG GCCAAGCAAA TTACCAGTCT GCTGGAAGAC CTGAAGAGGA TGAAATGTAT 
GAAGCTGCCA TAATAGAAAC TAATTGCATT TACCGTCTTG TAGAGGATAA ACTCATTCCT 
GAAGATGACT ACTGGGGAAA GATGCCAAAA TGTACCCTGC TACAACCAAA AGAGGTGCTC 
GTATTTGATT TTGGCAGTGA AGTGTATGTC TGGCATGGAA AAGAAGTTAC TTTAGCACAA 
AGAAAAGTGG CATTCCAGCT GGCAAAACAC TTATGGAATG GCACCTTCGA CTACTCAAAT 
TGTGACATAA ATCCTCTGGA CCCAGGAGAA TGCAATCCCC TCATACCCAG AAAGGGACAA 
GGACGTCCAG ACTGGGCTGT GTTTGGCAGG CTCACAGAAC ATAATGAGAC CATACTGTTC 
AAAGAAAAAT TCCTTGATTG GACAGAGCTG AAGAAACTCA ATGAGAAGAA TTCTAGTGAA 
TCCCTTCACC AGAAGGAAGA ATCCAAATCT GATTCTAAAC CATATGATGT CATGCTAATG 
GTACCTGTGC CCCAAACTGC TGTAGGCACG GTTCTGGATG GAATGAATAT TGGCCGGGGC 
TATGGACTTG TAGAAGGAGA AGATGGGAGG CAGTTTGAAA TTATCACTGC ATCTGTGGAT 
GTCTGGCACA TCCTGGAGTT TGATTATAGT AGATTGCCTA AACAAAGCAT AGGACAGTTC 
CATGAGGGAG ACACATACGT GGTGAAGTGG AAGTACATGG TGAGCACTAC AGTTGGAAGC 
AGACAAAAAG GAGAGCAACA AGTAAGAGCT GTTGGAAAAG AGAAATGTGT GTATTTCTTT 
TGGCAAGGAA GGCACTCCAC AGTGAGTGAG AAAGGGACAT CGGCACTGAT GACAGTGGAA 
CTTGATGAGG AGAGAGGAGC ACAGGTACAA GTGCTTCAAG GGAAAGAACT ACCATGCTTC 
CTGCAGTGCT TCCAAGGAGG GATGATTGTG CATGCTGGAA GAAGGGAAGA GGAAGAAGAA 
AATGCACAAA GTGACTGGAG ACTATATTGC GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC 
TTACTTGAAG TAGCATGTCA CTGCAGCAGC CTGCGTTCCC GGACATCCAT GATTGTCCTC 
AATATAAATA AAGCTCTTAT CTACCTGTGG CATGGCTGCA AAGCACAATC TCACACCAAG 
GATGTAGGAA GAACAGCAGC CAATAAAATA AAAGAACAAT GTCCGCTGGA AGCAGGGCTG 
CACAGCAGCA GCAAGGTGAC AATACACGAA TGTGATGAAG GGTCAGAGCC TTTGGGATTC 
TGGGATGCAT TAGGAAGGCG AGATCGAAAG GCCTATGATT GCATGTTGCA AGATCCGGGA 
AAGTTTAATT TCACCCCCCG CCTCTTCAGC CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC 
ACTGAGTACG TTTACCCTTC AAGAGACCCT ACTGTCATCA ATTCTATGCC CTTCTTGCAA 
GAGGATCTTT ACACTGCCCC ACAGCCAGCA CTGTTCCTTG TTGACAATCA CCATGAAGTG 
TACCTGTGGC AAGGCTGGTG GCCTGTGGAA AACAAAATCA CTGGATCAGC TCGAATCAGG 
TGGGCTACAG ACAGGAAATG CGCCATGGAG ACCGTGCTCC AGTACTGCAA AGGAAAAAAT 
GTAAAGAAGC CCCCAAAGTC CTATCTCATT CATGCCGGCC TAGAGCCTCT GACCTTCACT 
AATATGTTCC CAAGTTGGGA ACACAGGGAA GACATTGCAG AGATCACAGA AATGGATGCT 
GACGTTTCTA ATCAGATAAT CCTTGTAGAA GATGTGCTGG CCAAGCTGTG TAAAACTGTG 
TATCCCCTAG CTGATCTCTT AGCCAGGCCT TTACCTGAGG GAGTTGATCC TCTGAAGCTT 
GAAATTTATC TTTCAGATGA GGACTTTGAG GTTGCATTAG AGATGACAAG AGAAGAGTAC 
AATGCGCTGC CATCTTGGAA GCAGGTTAAT CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939256.1
CDS	17..6853
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_004939199.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939199.2

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGCA GAGGATAGCA 
GATAATCAGG AGAGTCAAAT GACTATAGAA GAAAGAAAAC ACCTCATCAC AGCTCGAGAA 
GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA GCAAATGACT CCACGCAGTT CACCGTAGCT 
GGTCGAATGG TAAAAAAAGG CCTGGCTTCT CCAAGTGCAC TAACACCAGT AGCATCCCCT 
TATGGTAACA GGCAGAAGTC TACAACTCCG GTCACAAAGC CCTTGGAAGA AATTGAAGCT 
AGGCCTGATA TGCAATTGGA ATCAGATATG AAGCTTGACA AGCTGGAGTC ATTCTTGGGA 
AGGCTGAATA ACAAAGTCGC TGGAATGCAA GAGACAGTGT TGACAGTTAC AGGAAAATCT 
GTGAAAGAGG TGATGAAGCT GGATGATGAT GAAACATTTT CCAAATTTTA CCGCCGTGTG 
GATTTCCCTT CCTCCTCTGT GCCTTTGGAC CTTGATGAGG ACTTTGATGC CATCTTTGAC 
CCTTATGCAC CAAAGTTAAT ATCCTCTGTA GCAGAGCACA AACGTGCAGT GAGGCCTATG 
CGCAGGGTTC AGTCCTCCAG AAACCCCCTG AAAATGCTGG CAGCAAGAGA AGATATTCTT 
CATGAGTACA CTGAACAAAG ACTGAATGTT GCCTTCATGG AGTCAAAGCG AATGAAAGTA 
GAAAAAATGT CTGCAAACTC AAATTTCTCA GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG 
GAGAACTTCA GCAACGTTAG TCTGCGGAGT GTTAACCTAA CAGAGCAAAA TTCTAACAAC 
AGTGCTGTGC CATACAAGAA GTTAATGCTG CTGCAAATTA AAGGAAGAAG ACATGTTCAA 
ACAAGATTAG TTGAACCAAG GGCCTCATCA CTGAACAGTG GAGACTGTTT CCTATTGTTA 
ACTCCACATC TCTGTTTCTT ATGGGTAGGA GAGTTTGCAA ATGTCATAGA AAAAGCAAAG 
GCTTCAGAAC TTGCAACTTT GATTCAGACA AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT 
ATACAGACTA TAGAAGAAGG AATAAACACC CATACCCATG CAGCCAAAGA CTTCTGGAAA 
CTCCTTGGTG GCCAAGCAAA TTACCAGTCT GCTGGAAGAC CTGAAGAGGA TGAAATGTAT 
GAAGCTGCCA TAATAGAAAC TAATTGCATT TACCGTCTTG TAGAGGATAA ACTCATTCCT 
GAAGATGACT ACTGGGGAAA GATGCCAAAA TGTACCCTGC TACAACCAAA AGAGGTGCTC 
GTATTTGATT TTGGCAGTGA AGTGTATGTC TGGCATGGAA AAGAAGTTAC TTTAGCACAA 
AGAAAAGTGG CATTCCAGCT GGCAAAACAC TTATGGAATG GCACCTTCGA CTACTCAAAT 
TGTGACATAA ATCCTCTGGA CCCAGGAGAA TGCAATCCCC TCATACCCAG AAAGGGACAA 
GGACGTCCAG ACTGGGCTGT GTTTGGCAGG CTCACAGAAC ATAATGAGAC CATACTGTTC 
AAAGAAAAAT TCCTTGATTG GACAGAGCTG AAGAAACTCA ATGAGAAGAA TTCTAGTGAA 
TCCCTTCACC AGAAGGAAGA ATCCAAATCT GATTCTAAAC CATATGATGT CATGCTAATG 
GTACCTGTGC CCCAAACTGC TGTAGGCACG GTTCTGGATG GAATGAATAT TGGCCGGGGC 
TATGGACTTG TAGAAGGAGA AGATGGGAGG CAGTTTGAAA TTATCACTGC ATCTGTGGAT 
GTCTGGCACA TCCTGGAGTT TGATTATAGT AGATTGCCTA AACAAAGCAT AGGACAGTTC 
CATGAGGGAG ACACATACGT GGTGAAGTGG AAGTACATGG TGAGCACTAC AGTTGGAAGC 
AGACAAAAAG GAGAGCAACA AGTAAGAGCT GTTGGAAAAG AGAAATGTGT GTATTTCTTT 
TGGCAAGGAA GGCACTCCAC AGTGAGTGAG AAAGGGACAT CGGCACTGAT GACAGTGGAA 
CTTGATGAGG AGAGAGGAGC ACAGGTACAA GTGCTTCAAG GGAAAGAACT ACCATGCTTC 
CTGCAGTGCT TCCAAGGAGG GATGATTGTG CATGCTGGAA GAAGGGAAGA GGAAGAAGAA 
AATGCACAAA GTGACTGGAG ACTATATTGC GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC 
TTACTTGAAG TAGCATGTCA CTGCAGCAGC CTGCGTTCCC GGACATCCAT GATTGTCCTC 
AATATAAATA AAGCTCTTAT CTACCTGTGG CATGGCTGCA AAGCACAATC TCACACCAAG 
GATGTAGGAA GAACAGCAGC CAATAAAATA AAAGAACAAT GTCCGCTGGA AGCAGGGCTG 
CACAGCAGCA GCAAGGTGAC AATACACGAA TGTGATGAAG GGTCAGAGCC TTTGGGATTC 
TGGGATGCAT TAGGAAGGCG AGATCGAAAG GCCTATGATT GCATGTTGCA AGATCCGGGA 
AAGTTTAATT TCACCCCCCG CCTCTTCAGC CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC 
ACTGAGTACG TTTACCCTTC AAGAGACCCT ACTGTCATCA ATTCTATGCC CTTCTTGCAA 
GAGGATCTTT ACACTGCCCC ACAGCCAGCA CTGTTCCTTG TTGACAATCA CCATGAAGTG 
TACCTGTGGC AAGGCTGGTG GCCTGTGGAA AACAAAATCA CTGGATCAGC TCGAATCAGG 
TGGGCTACAG ACAGGAAATG CGCCATGGAG ACCGTGCTCC AGTACTGCAA AGGAAAAAAT 
GTAAAGAAGC CCCCAAAGTC CTATCTCATT CATGCCGGCC TAGAGCCTCT GACCTTCACT 
AATATGTTCC CAAGTTGGGA ACACAGGGAA GACATTGCAG AGATCACAGA AATGGATGCT 
GACGTTTCTA ATCAGATAAT CCTTGTAGAA GATGTGCTGG CCAAGCTGTG TAAAACTGTG 
TATCCCCTAG CTGATCTCTT AGCCAGGCCT TTACCTGAGG GAGTTGATCC TCTGAAGCTT 
GAAATTTATC TTTCAGATGA GGACTTTGAG GTTGCATTAG AGATGACAAG AGAAGAGTAC 
AATGCGCTGC CATCTTGGAA GCAGGTTAAT CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_004939199.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939256.1 Gallus gallus supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OGa04064
Clone ID Related Accession (Same CDS sequence)	XM_004939199.2 , XM_004939199.3
Accession Version	XM_004939199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGCA GAGGATAGCA 
GATAATCAGG AGAGTCAAAT GACTATAGAA GAAAGAAAAC ACCTCATCAC AGCTCGAGAA 
GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA GCAAATGACT CCACGCAGTT CACCGTAGCT 
GGTCGAATGG TAAAAAAAGG CCTGGCTTCT CCAAGTGCAC TAACACCAGT AGCATCCCCT 
TATGGTAACA GGCAGAAGTC TACAACTCCG GTCACAAAGC CCTTGGAAGA AATTGAAGCT 
AGGCCTGATA TGCAATTGGA ATCAGATATG AAGCTTGACA AGCTGGAGTC ATTCTTGGGA 
AGGCTGAATA ACAAAGTCGC TGGAATGCAA GAGACAGTGT TGACAGTTAC AGGAAAATCT 
GTGAAAGAGG TGATGAAGCT GGATGATGAT GAAACATTTT CCAAATTTTA CCGCCGTGTG 
GATTTCCCTT CCTCCTCTGT GCCTTTGGAC CTTGATGAGG ACTTTGATGC CATCTTTGAC 
CCTTATGCAC CAAAGTTAAT ATCCTCTGTA GCAGAGCACA AACGTGCAGT GAGGCCTATG 
CGCAGGGTTC AGTCCTCCAG AAACCCCCTG AAAATGCTGG CAGCAAGAGA AGATATTCTT 
CATGAGTACA CTGAACAAAG ACTGAATGTT GCCTTCATGG AGTCAAAGCG AATGAAAGTA 
GAAAAAATGT CTGCAAACTC AAATTTCTCA GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG 
GAGAACTTCA GCAACGTTAG TCTGCGGAGT GTTAACCTAA CAGAGCAAAA TTCTAACAAC 
AGTGCTGTGC CATACAAGAA GTTAATGCTG CTGCAAATTA AAGGAAGAAG ACATGTTCAA 
ACAAGATTAG TTGAACCAAG GGCCTCATCA CTGAACAGTG GAGACTGTTT CCTATTGTTA 
ACTCCACATC TCTGTTTCTT ATGGGTAGGA GAGTTTGCAA ATGTCATAGA AAAAGCAAAG 
GCTTCAGAAC TTGCAACTTT GATTCAGACA AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT 
ATACAGACTA TAGAAGAAGG AATAAACACC CATACCCATG CAGCCAAAGA CTTCTGGAAA 
CTCCTTGGTG GCCAAGCAAA TTACCAGTCT GCTGGAAGAC CTGAAGAGGA TGAAATGTAT 
GAAGCTGCCA TAATAGAAAC TAATTGCATT TACCGTCTTG TAGAGGATAA ACTCATTCCT 
GAAGATGACT ACTGGGGAAA GATGCCAAAA TGTACCCTGC TACAACCAAA AGAGGTGCTC 
GTATTTGATT TTGGCAGTGA AGTGTATGTC TGGCATGGAA AAGAAGTTAC TTTAGCACAA 
AGAAAAGTGG CATTCCAGCT GGCAAAACAC TTATGGAATG GCACCTTCGA CTACTCAAAT 
TGTGACATAA ATCCTCTGGA CCCAGGAGAA TGCAATCCCC TCATACCCAG AAAGGGACAA 
GGACGTCCAG ACTGGGCTGT GTTTGGCAGG CTCACAGAAC ATAATGAGAC CATACTGTTC 
AAAGAAAAAT TCCTTGATTG GACAGAGCTG AAGAAACTCA ATGAGAAGAA TTCTAGTGAA 
TCCCTTCACC AGAAGGAAGA ATCCAAATCT GATTCTAAAC CATATGATGT CATGCTAATG 
GTACCTGTGC CCCAAACTGC TGTAGGCACG GTTCTGGATG GAATGAATAT TGGCCGGGGC 
TATGGACTTG TAGAAGGAGA AGATGGGAGG CAGTTTGAAA TTATCACTGC ATCTGTGGAT 
GTCTGGCACA TCCTGGAGTT TGATTATAGT AGATTGCCTA AACAAAGCAT AGGACAGTTC 
CATGAGGGAG ACACATACGT GGTGAAGTGG AAGTACATGG TGAGCACTAC AGTTGGAAGC 
AGACAAAAAG GAGAGCAACA AGTAAGAGCT GTTGGAAAAG AGAAATGTGT GTATTTCTTT 
TGGCAAGGAA GGCACTCCAC AGTGAGTGAG AAAGGGACAT CGGCACTGAT GACAGTGGAA 
CTTGATGAGG AGAGAGGAGC ACAGGTACAA GTGCTTCAAG GGAAAGAACT ACCATGCTTC 
CTGCAGTGCT TCCAAGGAGG GATGATTGTG CATGCTGGAA GAAGGGAAGA GGAAGAAGAA 
AATGCACAAA GTGACTGGAG ACTATATTGC GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC 
TTACTTGAAG TAGCATGTCA CTGCAGCAGC CTGCGTTCCC GGACATCCAT GATTGTCCTC 
AATATAAATA AAGCTCTTAT CTACCTGTGG CATGGCTGCA AAGCACAATC TCACACCAAG 
GATGTAGGAA GAACAGCAGC CAATAAAATA AAAGAACAAT GTCCGCTGGA AGCAGGGCTG 
CACAGCAGCA GCAAGGTGAC AATACACGAA TGTGATGAAG GGTCAGAGCC TTTGGGATTC 
TGGGATGCAT TAGGAAGGCG AGATCGAAAG GCCTATGATT GCATGTTGCA AGATCCGGGA 
AAGTTTAATT TCACCCCCCG CCTCTTCAGC CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC 
ACTGAGTACG TTTACCCTTC AAGAGACCCT ACTGTCATCA ATTCTATGCC CTTCTTGCAA 
GAGGATCTTT ACACTGCCCC ACAGCCAGCA CTGTTCCTTG TTGACAATCA CCATGAAGTG 
TACCTGTGGC AAGGCTGGTG GCCTGTGGAA AACAAAATCA CTGGATCAGC TCGAATCAGG 
TGGGCTACAG ACAGGAAATG CGCCATGGAG ACCGTGCTCC AGTACTGCAA AGGAAAAAAT 
GTAAAGAAGC CCCCAAAGTC CTATCTCATT CATGCCGGCC TAGAGCCTCT GACCTTCACT 
AATATGTTCC CAAGTTGGGA ACACAGGGAA GACATTGCAG AGATCACAGA AATGGATGCT 
GACGTTTCTA ATCAGATAAT CCTTGTAGAA GATGTGCTGG CCAAGCTGTG TAAAACTGTG 
TATCCCCTAG CTGATCTCTT AGCCAGGCCT TTACCTGAGG GAGTTGATCC TCTGAAGCTT 
GAAATTTATC TTTCAGATGA GGACTTTGAG GTTGCATTAG AGATGACAAG AGAAGAGTAC 
AATGCGCTGC CATCTTGGAA GCAGGTTAAT CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939256.1
CDS	19..6855
Translation	MHWMGKASCFLGHRSGVMILTMDADFNKGNSVRFKPSRLLHRKRALSFQKEFSFEDKEELANSTKDIDANLLAVLPKVSELRKLFEPNNQEFLEMKRKERIARRLEGIENDTQPMLLQNCPGSVTHRLLEEDTPRYMRATDPYSHHLGRSNEEEEASDSSVEKQPRSSRYRTETTGGNTESLYSTGNMDTHELESKAERIARYKAERRRQLAEKYGLSLDSDLDSDYSSRYSRARKDPDSVERKALRSERHDDENKDSGSLYLSRTEVKESKSVLSESREYSSREKDGIPAKEELSNEKSDKRADDDSAIRQASDPSATLDSSVSLTLSGRESSSCNEVPISPKQSPRESLSSPKRAASPIHLQNDQPLHSNIRQSSAGKAKPEWFLQKDSEGDTPSLISWPSRVKVREKLVKEESARGSPEPVTDSVSQRKSQPVSAHYMPLQTETSAFDRVLNQPVSAVRQPIHGYIQPAGVAHTAQLMATEEPESIFVGSRLLPDSVSPLTKSAAATAPEGEKKETLAYGAKPGEEESPEGEKASKGRRPILPSEIRKQGKSHEGLFGGEEPDTSVGSSSSASKPKVGSDVQQEQECKKKQAPEQQLKTPENMERSEAVMYIQSGSVSKPAQAKGPVWKVSKHKVLTRSMSDYTVPPKLEAFHSKSMEANAANGEIGMVDTKVSVAQLRNVFLETAHANKKTDLESRFEMSTSGSALSAGGDRERGPRRPRRYFTPGENRKTSERFKTQPITSAERKETDRSIMCAEIPTADDEEKLDDRAKLSVAAKRLLFREMEKSFEMKNIPKPPTRSSAVERRMRRLQDRSHTQPITSEEVVIAATETTPASRSVNTHTVVARVPSPTLVKSTVQPTSLQASAHQKILAKEEIRAAKEAMGQDQSDEPDSSTLSLAEKMALFNKLSQPVSRAISTRSRGDIRHRRMNTRYQTQPVTLGEVEQMQWKKSKPVQNESGKLTPLSSTVSTSVSAMASALSALFAGDMHAKSRSDGSVSAATKDELRFHASAESSDSSGKRTQKSEQWQPSMEVLESKRASKKHEEEGKRFLAHEANEIRKYSSFEDETTHPVLEKTGDYHKEAAYSFLRKGSMELFSSQPLFQPLERKEIDTIMQDHVEPTSELTSTPATRTLSQTTAAASCRLQELSEQLEGKFYKNSCEMFSAGENKIQTTEDATDSSSKTMSIKERLALLKKSGEEDWKSRLSKKQEYAKVSATDRSAQMQEVEQLLKKQRIADNQESQMTIEERKHLITAREEAWKSRGKGAANDSTQFTVAGRMVKKGLASPSALTPVASPYGNRQKSTTPVTKPLEEIEARPDMQLESDMKLDKLESFLGRLNNKVAGMQETVLTVTGKSVKEVMKLDDDETFSKFYRRVDFPSSSVPLDLDEDFDAIFDPYAPKLISSVAEHKRAVRPMRRVQSSRNPLKMLAAREDILHEYTEQRLNVAFMESKRMKVEKMSANSNFSEVALAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQIKGRRHVQTRLVEPRASSLNSGDCFLLLTPHLCFLWVGEFANVIEKAKASELATLIQTKRELGCRASYIQTIEEGINTHTHAAKDFWKLLGGQANYQSAGRPEEDEMYEAAIIETNCIYRLVEDKLIPEDDYWGKMPKCTLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYSNCDINPLDPGECNPLIPRKGQGRPDWAVFGRLTEHNETILFKEKFLDWTELKKLNEKNSSESLHQKEESKSDSKPYDVMLMVPVPQTAVGTVLDGMNIGRGYGLVEGEDGRQFEIITASVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMVSTTVGSRQKGEQQVRAVGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKELPCFLQCFQGGMIVHAGRREEEEENAQSDWRLYCVRGEVPNEGNLLEVACHCSSLRSRTSMIVLNINKALIYLWHGCKAQSHTKDVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFSATEYVYPSRDPTVINSMPFLQEDLYTAPQPALFLVDNHHEVYLWQGWWPVENKITGSARIRWATDRKCAMETVLQYCKGKNVKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDADVSNQIILVEDVLAKLCKTVYPLADLLARPLPEGVDPLKLEIYLSDEDFEVALEMTREEYNALPSWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_004939199.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939199.3

ORF Insert Sequence:

ATGCACTGGA TGGGTAAGGC TTCCTGCTTC CTGGGACACA GAAGTGGGGT CATGATTCTA 
ACAATGGATG CTGATTTTAA CAAAGGAAAC TCGGTTAGAT TTAAACCTTC ACGACTGCTC 
CACAGGAAGA GAGCGTTAAG TTTTCAGAAG GAGTTCTCAT TTGAAGATAA AGAAGAACTT 
GCAAACAGCA CAAAGGATAT TGATGCAAAC CTCTTAGCAG TACTTCCAAA AGTTTCAGAA 
TTAAGAAAAC TGTTTGAACC AAACAACCAA GAGTTTTTGG AAATGAAAAG AAAGGAGAGA 
ATAGCTAGAC GCTTAGAGGG AATTGAAAAT GATACGCAAC CTATGCTTCT ACAGAACTGC 
CCAGGGTCAG TCACACACCG TTTACTAGAA GAAGACACAC CAAGGTATAT GCGTGCAACT 
GATCCATACA GTCATCACTT AGGAAGATCA AATGAAGAGG AGGAAGCTTC AGATTCTTCT 
GTAGAGAAAC AACCCAGATC ATCCAGATAC CGCACAGAAA CCACAGGAGG AAATACAGAG 
TCTTTGTACA GTACAGGAAA CATGGATACC CATGAACTAG AGTCTAAAGC AGAAAGAATA 
GCTAGGTACA AGGCAGAGAG ACGGCGCCAA CTGGCAGAAA AGTATGGATT ATCTCTTGAT 
TCTGATTTGG ATTCTGACTA CTCATCCAGA TATTCCCGGG CAAGAAAAGA TCCTGACAGT 
GTGGAAAGAA AGGCATTGAG AAGTGAAAGG CATGATGATG AAAACAAGGA CTCTGGCTCC 
TTGTATTTGT CCAGGACTGA GGTGAAGGAG TCAAAGAGTG TGCTTTCTGA GTCCAGGGAG 
TACTCTAGCC GTGAAAAAGA TGGTATTCCA GCTAAAGAAG AGCTTTCAAA TGAAAAGAGT 
GATAAAAGAG CAGATGATGA CAGTGCCATT AGACAGGCTT CTGACCCTTC AGCAACATTG 
GATAGTTCTG TCTCACTAAC ACTTTCTGGA CGAGAGTCTT CCTCCTGTAA TGAAGTGCCA 
ATATCACCAA AGCAAAGCCC CCGAGAGTCT CTTTCTTCAC CAAAGCGGGC AGCCTCACCA 
ATCCATTTGC AGAATGATCA GCCCTTGCAC AGTAACATCA GACAAAGTAG TGCAGGGAAA 
GCAAAACCTG AATGGTTCCT TCAGAAAGAT TCAGAAGGGG ACACACCATC TCTTATCAGC 
TGGCCCTCCA GAGTAAAAGT TAGAGAGAAA CTGGTGAAAG AGGAAAGTGC ACGTGGAAGC 
CCTGAACCTG TCACAGACAG TGTATCTCAG AGAAAATCCC AGCCAGTGTC AGCCCACTAC 
ATGCCTCTTC AGACAGAAAC GTCTGCCTTT GATAGGGTTC TCAATCAGCC CGTCAGTGCA 
GTCCGCCAAC CAATACATGG GTATATTCAG CCTGCTGGTG TTGCACACAC GGCTCAGCTG 
ATGGCAACAG AAGAACCAGA AAGCATCTTC GTTGGCAGCC GCCTCCTTCC AGACAGTGTC 
AGCCCCTTAA CGAAATCAGC AGCAGCCACT GCACCAGAGG GTGAAAAGAA AGAGACGCTG 
GCTTATGGAG CAAAGCCTGG CGAAGAAGAA TCCCCAGAGG GTGAGAAGGC TTCCAAAGGC 
AGAAGACCAA TTTTGCCATC TGAGATTCGC AAGCAAGGGA AGAGCCACGA AGGCCTCTTT 
GGAGGAGAAG AACCAGATAC CAGTGTGGGG AGCTCCTCAT CTGCAAGCAA GCCGAAGGTT 
GGCTCAGATG TTCAGCAGGA GCAGGAATGC AAAAAGAAGC AGGCGCCTGA GCAGCAGCTG 
AAGACACCTG AGAACATGGA GAGGAGCGAA GCTGTCATGT ACATACAGAG CGGGTCTGTA 
TCAAAGCCAG CCCAGGCAAA GGGCCCCGTT TGGAAGGTGT CAAAGCATAA AGTCCTGACT 
CGCTCCATGT CTGACTATAC TGTGCCTCCT AAGCTAGAAG CTTTTCATAG TAAGAGCATG 
GAAGCAAACG CAGCAAATGG TGAGATTGGC ATGGTTGACA CAAAGGTCTC TGTTGCCCAG 
CTCCGTAATG TCTTTCTGGA AACTGCTCAT GCCAACAAGA AAACGGACCT CGAATCTCGG 
TTTGAGATGT CAACGTCAGG CAGCGCTTTG TCTGCCGGTG GTGACCGTGA AAGAGGGCCG 
CGAAGGCCGA GGCGCTATTT CACTCCTGGT GAAAATAGAA AGACTTCTGA GCGATTTAAA 
ACCCAACCTA TAACGTCAGC AGAACGAAAG GAGACGGATA GGTCTATTAT GTGTGCTGAA 
ATACCAACTG CTGACGATGA AGAAAAATTA GATGACCGAG CCAAACTAAG TGTAGCTGCA 
AAGAGACTGC TTTTTAGAGA AATGGAAAAA TCTTTTGAAA TGAAGAATAT TCCTAAACCA 
CCTACGAGAA GCTCAGCAGT AGAGAGAAGA ATGCGGCGTT TGCAGGACAG ATCACACACG 
CAGCCTATTA CCAGTGAGGA AGTGGTCATT GCAGCTACTG AAACTACCCC TGCTTCACGT 
TCTGTGAATA CCCACACAGT AGTGGCAAGA GTACCTAGTC CCACTCTAGT TAAGAGCACT 
GTACAACCTA CCAGCTTGCA GGCATCAGCA CACCAAAAAA TACTAGCTAA AGAGGAGATA 
AGAGCAGCCA AAGAGGCGAT GGGGCAAGAT CAATCCGATG AACCAGATTC TTCCACATTA 
AGCTTGGCAG AAAAGATGGC TTTGTTTAAC AAACTGTCTC AGCCAGTATC CAGGGCTATA 
TCCACAAGGA GTCGGGGAGA TATTAGGCAC AGAAGGATGA ATACTCGTTA CCAGACCCAG 
CCAGTCACTC TTGGAGAAGT TGAGCAGATG CAATGGAAAA AGAGTAAACC GGTGCAAAAC 
GAAAGTGGAA AACTAACTCC CCTGTCAAGT ACAGTTTCAA CATCAGTTTC AGCAATGGCT 
TCTGCCCTTT CTGCACTGTT TGCTGGAGAC ATGCATGCGA AGTCACGTAG TGATGGAAGT 
GTGAGCGCTG CCACAAAAGA TGAGTTGAGA TTCCATGCTT CAGCAGAAAG CTCAGACTCA 
TCAGGAAAAC GCACACAGAA GTCAGAACAG TGGCAGCCCT CAATGGAAGT GCTGGAAAGC 
AAAAGAGCAT CAAAGAAGCA TGAAGAAGAA GGAAAGAGGT TTCTAGCACA TGAGGCTAAT 
GAAATCAGAA AGTACAGCTC CTTTGAGGAT GAAACCACAC ATCCTGTTCT TGAAAAAACA 
GGAGACTACC ATAAAGAGGC AGCCTACAGC TTCCTGAGGA AGGGCAGCAT GGAACTGTTT 
AGTTCACAAC CACTTTTTCA GCCTCTTGAA AGGAAGGAAA TTGATACCAT CATGCAAGAC 
CACGTGGAGC CTACCTCTGA ACTCACCAGC ACACCAGCAA CGAGGACACT TTCCCAGACT 
ACAGCTGCAG CATCCTGTAG GCTACAGGAG CTGTCTGAAC AACTGGAGGG AAAGTTCTAC 
AAGAATTCCT GTGAGATGTT TTCTGCTGGA GAGAACAAAA TACAGACAAC TGAGGATGCC 
ACCGATAGCT CCAGCAAAAC GATGTCAATT AAAGAAAGGT TGGCATTGCT GAAGAAAAGT 
GGTGAAGAAG ATTGGAAGAG CAGGCTCAGT AAAAAGCAGG AATATGCAAA AGTGTCTGCC 
ACTGATCGTA GCGCACAGAT GCAAGAAGTT GAGCAGCTGT TGAAAAAGCA GAGGATAGCA 
GATAATCAGG AGAGTCAAAT GACTATAGAA GAAAGAAAAC ACCTCATCAC AGCTCGAGAA 
GAAGCCTGGA AGTCCAGAGG TAAAGGCGCA GCAAATGACT CCACGCAGTT CACCGTAGCT 
GGTCGAATGG TAAAAAAAGG CCTGGCTTCT CCAAGTGCAC TAACACCAGT AGCATCCCCT 
TATGGTAACA GGCAGAAGTC TACAACTCCG GTCACAAAGC CCTTGGAAGA AATTGAAGCT 
AGGCCTGATA TGCAATTGGA ATCAGATATG AAGCTTGACA AGCTGGAGTC ATTCTTGGGA 
AGGCTGAATA ACAAAGTCGC TGGAATGCAA GAGACAGTGT TGACAGTTAC AGGAAAATCT 
GTGAAAGAGG TGATGAAGCT GGATGATGAT GAAACATTTT CCAAATTTTA CCGCCGTGTG 
GATTTCCCTT CCTCCTCTGT GCCTTTGGAC CTTGATGAGG ACTTTGATGC CATCTTTGAC 
CCTTATGCAC CAAAGTTAAT ATCCTCTGTA GCAGAGCACA AACGTGCAGT GAGGCCTATG 
CGCAGGGTTC AGTCCTCCAG AAACCCCCTG AAAATGCTGG CAGCAAGAGA AGATATTCTT 
CATGAGTACA CTGAACAAAG ACTGAATGTT GCCTTCATGG AGTCAAAGCG AATGAAAGTA 
GAAAAAATGT CTGCAAACTC AAATTTCTCA GAAGTAGCAC TTGCTGGTTT GGCAAGCAAG 
GAGAACTTCA GCAACGTTAG TCTGCGGAGT GTTAACCTAA CAGAGCAAAA TTCTAACAAC 
AGTGCTGTGC CATACAAGAA GTTAATGCTG CTGCAAATTA AAGGAAGAAG ACATGTTCAA 
ACAAGATTAG TTGAACCAAG GGCCTCATCA CTGAACAGTG GAGACTGTTT CCTATTGTTA 
ACTCCACATC TCTGTTTCTT ATGGGTAGGA GAGTTTGCAA ATGTCATAGA AAAAGCAAAG 
GCTTCAGAAC TTGCAACTTT GATTCAGACA AAGAGGGAAC TTGGCTGTAG AGCTTCTTAT 
ATACAGACTA TAGAAGAAGG AATAAACACC CATACCCATG CAGCCAAAGA CTTCTGGAAA 
CTCCTTGGTG GCCAAGCAAA TTACCAGTCT GCTGGAAGAC CTGAAGAGGA TGAAATGTAT 
GAAGCTGCCA TAATAGAAAC TAATTGCATT TACCGTCTTG TAGAGGATAA ACTCATTCCT 
GAAGATGACT ACTGGGGAAA GATGCCAAAA TGTACCCTGC TACAACCAAA AGAGGTGCTC 
GTATTTGATT TTGGCAGTGA AGTGTATGTC TGGCATGGAA AAGAAGTTAC TTTAGCACAA 
AGAAAAGTGG CATTCCAGCT GGCAAAACAC TTATGGAATG GCACCTTCGA CTACTCAAAT 
TGTGACATAA ATCCTCTGGA CCCAGGAGAA TGCAATCCCC TCATACCCAG AAAGGGACAA 
GGACGTCCAG ACTGGGCTGT GTTTGGCAGG CTCACAGAAC ATAATGAGAC CATACTGTTC 
AAAGAAAAAT TCCTTGATTG GACAGAGCTG AAGAAACTCA ATGAGAAGAA TTCTAGTGAA 
TCCCTTCACC AGAAGGAAGA ATCCAAATCT GATTCTAAAC CATATGATGT CATGCTAATG 
GTACCTGTGC CCCAAACTGC TGTAGGCACG GTTCTGGATG GAATGAATAT TGGCCGGGGC 
TATGGACTTG TAGAAGGAGA AGATGGGAGG CAGTTTGAAA TTATCACTGC ATCTGTGGAT 
GTCTGGCACA TCCTGGAGTT TGATTATAGT AGATTGCCTA AACAAAGCAT AGGACAGTTC 
CATGAGGGAG ACACATACGT GGTGAAGTGG AAGTACATGG TGAGCACTAC AGTTGGAAGC 
AGACAAAAAG GAGAGCAACA AGTAAGAGCT GTTGGAAAAG AGAAATGTGT GTATTTCTTT 
TGGCAAGGAA GGCACTCCAC AGTGAGTGAG AAAGGGACAT CGGCACTGAT GACAGTGGAA 
CTTGATGAGG AGAGAGGAGC ACAGGTACAA GTGCTTCAAG GGAAAGAACT ACCATGCTTC 
CTGCAGTGCT TCCAAGGAGG GATGATTGTG CATGCTGGAA GAAGGGAAGA GGAAGAAGAA 
AATGCACAAA GTGACTGGAG ACTATATTGC GTGCGAGGAG AAGTTCCCAA TGAAGGAAAC 
TTACTTGAAG TAGCATGTCA CTGCAGCAGC CTGCGTTCCC GGACATCCAT GATTGTCCTC 
AATATAAATA AAGCTCTTAT CTACCTGTGG CATGGCTGCA AAGCACAATC TCACACCAAG 
GATGTAGGAA GAACAGCAGC CAATAAAATA AAAGAACAAT GTCCGCTGGA AGCAGGGCTG 
CACAGCAGCA GCAAGGTGAC AATACACGAA TGTGATGAAG GGTCAGAGCC TTTGGGATTC 
TGGGATGCAT TAGGAAGGCG AGATCGAAAG GCCTATGATT GCATGTTGCA AGATCCGGGA 
AAGTTTAATT TCACCCCCCG CCTCTTCAGC CTCAGTAGTT CTTCGGGAGA ATTCTCAGCC 
ACTGAGTACG TTTACCCTTC AAGAGACCCT ACTGTCATCA ATTCTATGCC CTTCTTGCAA 
GAGGATCTTT ACACTGCCCC ACAGCCAGCA CTGTTCCTTG TTGACAATCA CCATGAAGTG 
TACCTGTGGC AAGGCTGGTG GCCTGTGGAA AACAAAATCA CTGGATCAGC TCGAATCAGG 
TGGGCTACAG ACAGGAAATG CGCCATGGAG ACCGTGCTCC AGTACTGCAA AGGAAAAAAT 
GTAAAGAAGC CCCCAAAGTC CTATCTCATT CATGCCGGCC TAGAGCCTCT GACCTTCACT 
AATATGTTCC CAAGTTGGGA ACACAGGGAA GACATTGCAG AGATCACAGA AATGGATGCT 
GACGTTTCTA ATCAGATAAT CCTTGTAGAA GATGTGCTGG CCAAGCTGTG TAAAACTGTG 
TATCCCCTAG CTGATCTCTT AGCCAGGCCT TTACCTGAGG GAGTTGATCC TCTGAAGCTT 
GAAATTTATC TTTCAGATGA GGACTTTGAG GTTGCATTAG AGATGACAAG AGAAGAGTAC 
AATGCGCTGC CATCTTGGAA GCAGGTTAAT CTGAAAAAGG CAAAAGGACT TTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

SVIL cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: