NFASC cDNA ORF clone, Gallus gallus(chicken)

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa46526 XM_015298919.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa147938 XM_025143685.1
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa147939 XM_025143686.1
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa147940 XM_025143688.1
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa46512 XM_015298903.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46512 XM_015298904.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46512 XM_015298905.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46513 XM_015298906.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46514 XM_015298907.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46515 XM_015298908.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46516 XM_015298909.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46517 XM_015298910.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46518 XM_015298911.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46519 XM_015298912.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46520 XM_015298913.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46521 XM_015298914.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46522 XM_015298915.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46523 XM_015298916.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46524 XM_015298917.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa46525 XM_015298918.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa46527 XM_015298920.2
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa28259 NM_001160320.3
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa28267 NM_001160322.3
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa28270 NM_001004493.4
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa28275 NM_001160321.3
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa28276 NM_001160323.3
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa28282 NM_001160324.3
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa28289 NM_001004709.4
Latest version!
Gallus gallus neurofascin (NFASC), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa46512 XM_015298905.1 Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46515 XM_015298908.1 Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46521 XM_015298914.1 Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46522 XM_015298915.1 Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46527 XM_015298920.1 Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46512 XM_015298904.1 Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46526 XM_015298919.1 Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa46514 XM_015298907.1 Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46524 XM_015298917.1 Gallus gallus neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa46525 XM_015298918.1 Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa46513 XM_015298906.1 Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46518 XM_015298911.1 Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46520 XM_015298913.1 Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46516 XM_015298909.1 Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46523 XM_015298916.1 Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa46512 XM_015298903.1 Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46517 XM_015298910.1 Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa46519 XM_015298912.1 Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa46526
    Clone ID Related Accession (Same CDS sequence) XM_015298919.2 , XM_015298919.1
    Accession Version XM_015298919.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154405.1
    CDS169..3117
    Translation

    Target ORF information:

    RefSeq Version XM_015298919.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298919.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46526
    Clone ID Related Accession (Same CDS sequence) XM_015298919.2 , XM_015298919.1
    Accession Version XM_015298919.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154405.1
    CDS159..3107
    Translation

    Target ORF information:

    RefSeq Version XM_015298919.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147938
    Clone ID Related Accession (Same CDS sequence) XM_025143685.1
    Accession Version XM_025143685.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC
    AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG
    GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC
    ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA
    GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999453.1
    CDS169..3975
    Translation

    Target ORF information:

    RefSeq Version XM_025143685.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143685.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC
    AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG
    GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC
    ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA
    GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147939
    Clone ID Related Accession (Same CDS sequence) XM_025143686.1
    Accession Version XM_025143686.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC
    AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG
    GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC
    ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA
    GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999454.1
    CDS169..3735
    Translation

    Target ORF information:

    RefSeq Version XM_025143686.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143686.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGCGATG AAGACAACAA ACCACTGCCC
    AACAGTCAGA CCTCCCTGGA TGGCACGATA AAGCAACAGG AGAGTGACGA CAGCTTGGTG
    GACTACGGAG AGGGTGGGGA AGGGCAGTTC AACGAGGACG GCTCCTTCAT TGGCCAGTAC
    ACAGTGAAAA AGGACAAGGA GGAAACGGAA GGCAACGAGA GCTCAGAAGC CACGTCCCCA
    GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147940
    Clone ID Related Accession (Same CDS sequence) XM_025143688.1
    Accession Version XM_025143688.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA
    GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC
    ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC
    CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC
    TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA
    GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA
    GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT
    GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC
    AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC
    GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC
    GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA
    AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG
    CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC
    TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG
    CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG
    CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC
    TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT
    CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG
    GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA
    GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA
    GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCTT ACACCAAGAA CCACGTGGAC
    ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT
    CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC
    AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG
    TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC
    ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG
    CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA
    ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999456.1
    CDS169..3711
    Translation

    Target ORF information:

    RefSeq Version XM_025143688.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143688.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA
    GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC
    ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC
    CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC
    TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA
    GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA
    GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT
    GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC
    AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC
    GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC
    GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA
    AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG
    CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC
    TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG
    CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG
    CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC
    TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT
    CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG
    GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA
    GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA
    GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCTT ACACCAAGAA CCACGTGGAC
    ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT
    CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC
    AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG
    TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC
    ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG
    CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA
    ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46512
    Clone ID Related Accession (Same CDS sequence) XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
    Accession Version XM_015298905.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154391.1
    CDS95..4186
    Translation

    Target ORF information:

    RefSeq Version XM_015298905.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46512
    Clone ID Related Accession (Same CDS sequence) XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
    Accession Version XM_015298903.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154389.1
    CDS169..4260
    Translation

    Target ORF information:

    RefSeq Version XM_015298903.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298903.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46512
    Clone ID Related Accession (Same CDS sequence) XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
    Accession Version XM_015298905.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154391.1
    CDS91..4182
    Translation

    Target ORF information:

    RefSeq Version XM_015298905.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298905.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46512
    Clone ID Related Accession (Same CDS sequence) XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
    Accession Version XM_015298904.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154390.1
    CDS354..4445
    Translation

    Target ORF information:

    RefSeq Version XM_015298904.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298904.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46512
    Clone ID Related Accession (Same CDS sequence) XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
    Accession Version XM_015298904.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154390.1
    CDS367..4458
    Translation

    Target ORF information:

    RefSeq Version XM_015298904.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46512
    Clone ID Related Accession (Same CDS sequence) XM_015298905.1 , XM_015298903.2 , XM_015298905.2 , XM_015298904.2 , XM_015298904.1 , XM_015298903.1
    Accession Version XM_015298903.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154389.1
    CDS159..4250
    Translation

    Target ORF information:

    RefSeq Version XM_015298903.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGGT CTCTTGAAAG CGATGAAGAC AACAAACCAC TGCCCAACAG TCAGACCTCC
    CTGGATGGCA CGATAAAGCA ACAGGAGAGT GACGACAGCT TGGTGGACTA CGGAGAGGGT
    GGGGAAGGGC AGTTCAACGA GGACGGCTCC TTCATTGGCC AGTACACAGT GAAAAAGGAC
    AAGGAGGAAA CGGAAGGCAA CGAGAGCTCA GAAGCCACGT CCCCAGTCAA TGCTATCTAC
    TCATTAGCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46513
    Clone ID Related Accession (Same CDS sequence) XM_015298906.2 , XM_015298906.1
    Accession Version XM_015298906.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154392.1
    CDS169..4248
    Translation

    Target ORF information:

    RefSeq Version XM_015298906.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298906.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46513
    Clone ID Related Accession (Same CDS sequence) XM_015298906.2 , XM_015298906.1
    Accession Version XM_015298906.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154392.1
    CDS159..4238
    Translation

    Target ORF information:

    RefSeq Version XM_015298906.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCCACCAA TGGATCGTCC ATATGGGACA TAAGAGCTTG GGCTAATAGT
    AACTGGGCAA ACATCACCTG GAGCCACAAT TACTCTGCAG GAACTGACTT TGTGGTTAAG
    TATATCACCA GTAACAAGAC AGAGAAATCT ATCCCCGTTA AAGCTCAGAC CCCCTCCTCT
    GTGCAGCTGG CCAATCTGAC GCCGGGGATG GTGTACAAGC TGTGGGTGTT CCCCATATGG
    TCTAGCCCCA GCGAGCACTC GTACATAACC TTTACCACCA GCTCAGCTTA CACCAAGAAC
    CACGTGGACA TCGCGACCCA GGGCTGGTTC ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG
    GTCCTCATTC TGCTGATTGT TTGCTTCATT AAGAGGAGCA GAGGAGGGAA ATACCCAGTG
    CGTGACAACA AAGATGAGCA CCTGAATCCT GAAGACAAGA ACGTAGAAGA TGGCTCATTC
    GACTACAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46514
    Clone ID Related Accession (Same CDS sequence) XM_015298907.1 , XM_015298907.2
    Accession Version XM_015298907.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC
    ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT
    ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA
    AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC
    AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC
    TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG
    ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG
    ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC
    TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154393.1
    CDS159..4232
    Translation

    Target ORF information:

    RefSeq Version XM_015298907.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC
    ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT
    ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA
    AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC
    AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC
    TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG
    ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG
    ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC
    TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46514
    Clone ID Related Accession (Same CDS sequence) XM_015298907.1 , XM_015298907.2
    Accession Version XM_015298907.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC
    ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT
    ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA
    AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC
    AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC
    TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG
    ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG
    ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC
    TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154393.1
    CDS169..4242
    Translation

    Target ORF information:

    RefSeq Version XM_015298907.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298907.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CCTCCACCTG GTTGCCTCCA CCTACAACCG AGCTAACTCC AGCAGCCACC
    ATTGCCACCA CAACCACCAC TGCCACCCCT ACTACTGAAA CCCCTCCTAC TGAAATCCCT
    ACTACTGCCA TCCCTACCAC CACCACTACT ACAACCACCA CAGCTGCCAG CACCGTCGCA
    AGCACTACAA CAACTGCAGA GAGGGCTGCG GCAGCCACCA CAAAACAGGA GCTGGCCACC
    AATGGATCGT CCATATGGGA CATAAGAGCT TGGGCTAATA GTAACTGGGC AAACATCACC
    TGGAGCCACA ATTACTCTGC AGGAACTGAC TTTGTGGTTA AGTATATCAC CAGTAACAAG
    ACAGAGAAAT CTATCCCCGT TAAAGCTCAG ACCCCCTCCT CTGTGCAGCT GGCCAATCTG
    ACGCCGGGGA TGGTGTACAA GCTGTGGGTG TTCCCCATAT GGTCTAGCCC CAGCGAGCAC
    TCGTACATAA CCTTTACCAC CAGCTCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46515
    Clone ID Related Accession (Same CDS sequence) XM_015298908.1 , XM_015298908.2
    Accession Version XM_015298908.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA
    GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC
    ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC
    CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC
    TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA
    GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA
    GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT
    GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC
    AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC
    GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC
    GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA
    AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG
    CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC
    TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG
    CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG
    CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC
    TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT
    CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG
    GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA
    GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA
    GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC
    TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC
    ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC
    ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA
    GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG
    GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT
    GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC
    GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC
    AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC
    ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA
    CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC
    AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT
    GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC
    ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154394.1
    CDS159..4214
    Translation

    Target ORF information:

    RefSeq Version XM_015298908.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA
    GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC
    ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC
    CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC
    TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA
    GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA
    GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT
    GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC
    AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC
    GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC
    GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA
    AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG
    CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC
    TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG
    CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG
    CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC
    TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT
    CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG
    GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA
    GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA
    GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC
    TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC
    ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC
    ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA
    GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG
    GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT
    GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC
    GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC
    AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC
    ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA
    CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC
    AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT
    GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC
    ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46515
    Clone ID Related Accession (Same CDS sequence) XM_015298908.1 , XM_015298908.2
    Accession Version XM_015298908.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA
    GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC
    ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC
    CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC
    TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA
    GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA
    GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT
    GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC
    AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC
    GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC
    GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA
    AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG
    CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC
    TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG
    CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG
    CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC
    TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT
    CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG
    GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA
    GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA
    GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC
    TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC
    ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC
    ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA
    GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG
    GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT
    GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC
    GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC
    AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC
    ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA
    CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC
    AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT
    GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC
    ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154394.1
    CDS169..4224
    Translation

    Target ORF information:

    RefSeq Version XM_015298908.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298908.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGAGCGA CCCGACCGGC CCCGGGACCT GGAGCTGTCA
    GACCTGGCTG AGAGGAGCGT GAAGCTGACA TGGATTCCTG GCGATGACAA CAACAGCCCC
    ATCACAGACT ACATCGTCCA GTTTGAGGAG GACCGCTTCC AGCCTGGCAC GTGGCACAAC
    CACTCCAGGT ATCCTGGGAA TGTCAACTCG GCCCTCCTGA GCCTCTCTCC TTACGTCAAC
    TACCAATTTC GGGTGATTGC AGTGAACGAC GTGGGCAGCA GCCTGCCCAG CATGCCCTCA
    GAACGATACC AGACCAGCGG GGCACGACCT GAAATTAACC CAACAGGAGT TCAAGGAGCA
    GGGACCCAAA AAAACAACAT GGAGATAACC TGGACGCCTC TGAATGCAAC TCAAGCCTAT
    GGGCCCAACC TCCGTTACAT CGTGCGGTGG AGGCGAAGGG ACCCACGTGG GAGTTGGTAC
    AACGAGACGG TGAAGGCACC ACGACACGTC GTCTGGAACA CACCCATCTA CGTCCCCTAC
    GAGATCAAAG TGCAGGCAGA GAATGACTTT GGTAGAGCTC CAGAGCCTGA GACCTACATC
    GGCTACTCAG GGGAAGATTA TCCCAAGGCT GCACCTACAG ATGTTAGGAT AAGAGTTTTA
    AACAGCACTG CCATTGCTCT GACATGGACC CGCGTGCACC TGGACACCAT CCAGGGACAG
    CTTAAGGAGT ACAGAGCCTA TTTCTGGAGA GACAGTAGTT TGCTGAAGAA CCTGTGGGTC
    TCCAAAAAAC GGCAGTATGT GAGTTTTCCT GGAGACCGAA ACCGGGGCAT AGTGTCCCGG
    CTGTTCCCTT ACAGCAACTA CAAGCTTGAG ATGGTTGTAA CCAACGGGAG AGGCGATGGG
    CCCCGCAGCG AAGTCAAGGA GTTCCCCACA CCTGAAGGAG TGCCCAGCTC CCCCAGGTAC
    TTAAGAATCC GACAGCCAAA TCTGGAAAGC ATCAATTTGG AGTGGGATCA CCCAGAACAT
    CCCAATGGAG TCCTCACGGG ATACAACCTT AGATATCAAG CCTTTAACGG ATCCAAAACG
    GGCAGAACCC TGGTAGAGAA CTTCTCTCCC AACCAGACAA GGTTCACCGT GCAGAGGACA
    GACCCCATCT CGCGCTATCG ATTCTTCCTG CGTGCTCGGA CACAGGTGGG AGATGGAGAA
    GTCATAGTGG AAGAGTCACC AGCGCTCCTG AATGAAGCCA CGCCAACCCC AGCCTCCACC
    TGGTTGCCTC CACCTACAAC CGAGCTAACT CCAGCAGCCA CCATTGCCAC CACAACCACC
    ACTGCCACCC CTACTACTGA AACCCCTCCT ACTGAAATCC CTACTACTGC CATCCCTACC
    ACCACCACTA CTACAACCAC CACAGCTGCC AGCACCGTCG CAAGCACTAC AACAACTGCA
    GAGAGGGCTG CGGCAGCCAC CACAAAACAG GAGCTGGCCA CCAATGGATC GTCCATATGG
    GACATAAGAG CTTGGGCTAA TAGTAACTGG GCAAACATCA CCTGGAGCCA CAATTACTCT
    GCAGGAACTG ACTTTGTGGT TAAGTATATC ACCAGTAACA AGACAGAGAA ATCTATCCCC
    GTTAAAGCTC AGACCCCCTC CTCTGTGCAG CTGGCCAATC TGACGCCGGG GATGGTGTAC
    AAGCTGTGGG TGTTCCCCAT ATGGTCTAGC CCCAGCGAGC ACTCGTACAT AACCTTTACC
    ACCAGCTCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGGTCTCTTG AAAGCGATGA AGACAACAAA
    CCACTGCCCA ACAGTCAGAC CTCCCTGGAT GGCACGATAA AGCAACAGGA GAGTGACGAC
    AGCTTGGTGG ACTACGGAGA GGGTGGGGAA GGGCAGTTCA ACGAGGACGG CTCCTTCATT
    GGCCAGTACA CAGTGAAAAA GGACAAGGAG GAAACGGAAG GCAACGAGAG CTCAGAAGCC
    ACGTCCCCAG TCAATGCTAT CTACTCATTA GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46516
    Clone ID Related Accession (Same CDS sequence) XM_015298909.1 , XM_015298909.2
    Accession Version XM_015298909.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT
    CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC
    GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG
    AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT
    GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154395.1
    CDS159..4199
    Translation

    Target ORF information:

    RefSeq Version XM_015298909.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT
    CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC
    GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG
    AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT
    GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46516
    Clone ID Related Accession (Same CDS sequence) XM_015298909.1 , XM_015298909.2
    Accession Version XM_015298909.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT
    CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC
    GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG
    AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT
    GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154395.1
    CDS169..4209
    Translation

    Target ORF information:

    RefSeq Version XM_015298909.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGGTC TCTTGAAAGC GATGAAGACA ACAAACCACT GCCCAACAGT
    CAGACCTCCC TGGATGGCAC GATAAAGCAA CAGGAGAGTG ACGACAGCTT GGTGGACTAC
    GGAGAGGGTG GGGAAGGGCA GTTCAACGAG GACGGCTCCT TCATTGGCCA GTACACAGTG
    AAAAAGGACA AGGAGGAAAC GGAAGGCAAC GAGAGCTCAG AAGCCACGTC CCCAGTCAAT
    GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46517
    Clone ID Related Accession (Same CDS sequence) XM_015298910.1 , XM_015298910.2
    Accession Version XM_015298910.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC
    GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG
    ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC
    CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC
    CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG
    GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA
    ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG
    ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG
    CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC
    TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT
    AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA
    CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC
    GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC
    AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA
    GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG
    GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT
    GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC
    AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA
    TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC
    CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT
    GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT
    GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA
    GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT
    GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC
    ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG
    CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA
    AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC
    AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG
    GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC
    AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC
    ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT
    CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC
    AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG
    TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC
    ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG
    CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA
    ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154396.1
    CDS159..4181
    Translation

    Target ORF information:

    RefSeq Version XM_015298910.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC
    GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG
    ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC
    CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC
    CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG
    GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA
    ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG
    ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG
    CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC
    TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT
    AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA
    CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC
    GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC
    AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA
    GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG
    GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT
    GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC
    AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA
    TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC
    CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT
    GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT
    GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA
    GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT
    GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC
    ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG
    CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA
    AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC
    AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG
    GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC
    AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC
    ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT
    CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC
    AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG
    TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC
    ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG
    CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA
    ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46517
    Clone ID Related Accession (Same CDS sequence) XM_015298910.1 , XM_015298910.2
    Accession Version XM_015298910.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC
    GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG
    ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC
    CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC
    CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG
    GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA
    ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG
    ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG
    CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC
    TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT
    AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA
    CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC
    GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC
    AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA
    GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG
    GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT
    GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC
    AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA
    TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC
    CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT
    GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT
    GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA
    GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT
    GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC
    ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG
    CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA
    AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC
    AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG
    GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC
    AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC
    ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT
    CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC
    AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG
    TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC
    ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG
    CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA
    ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154396.1
    CDS169..4191
    Translation

    Target ORF information:

    RefSeq Version XM_015298910.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGCAAT CCCTGCTAAC CGTTTGAGAG ACTTACCTAA AGAGCGACCC
    GACCGGCCCC GGGACCTGGA GCTGTCAGAC CTGGCTGAGA GGAGCGTGAA GCTGACATGG
    ATTCCTGGCG ATGACAACAA CAGCCCCATC ACAGACTACA TCGTCCAGTT TGAGGAGGAC
    CGCTTCCAGC CTGGCACGTG GCACAACCAC TCCAGGTATC CTGGGAATGT CAACTCGGCC
    CTCCTGAGCC TCTCTCCTTA CGTCAACTAC CAATTTCGGG TGATTGCAGT GAACGACGTG
    GGCAGCAGCC TGCCCAGCAT GCCCTCAGAA CGATACCAGA CCAGCGGGGC ACGACCTGAA
    ATTAACCCAA CAGGAGTTCA AGGAGCAGGG ACCCAAAAAA ACAACATGGA GATAACCTGG
    ACGCCTCTGA ATGCAACTCA AGCCTATGGG CCCAACCTCC GTTACATCGT GCGGTGGAGG
    CGAAGGGACC CACGTGGGAG TTGGTACAAC GAGACGGTGA AGGCACCACG ACACGTCGTC
    TGGAACACAC CCATCTACGT CCCCTACGAG ATCAAAGTGC AGGCAGAGAA TGACTTTGGT
    AGAGCTCCAG AGCCTGAGAC CTACATCGGC TACTCAGGGG AAGATTATCC CAAGGCTGCA
    CCTACAGATG TTAGGATAAG AGTTTTAAAC AGCACTGCCA TTGCTCTGAC ATGGACCCGC
    GTGCACCTGG ACACCATCCA GGGACAGCTT AAGGAGTACA GAGCCTATTT CTGGAGAGAC
    AGTAGTTTGC TGAAGAACCT GTGGGTCTCC AAAAAACGGC AGTATGTGAG TTTTCCTGGA
    GACCGAAACC GGGGCATAGT GTCCCGGCTG TTCCCTTACA GCAACTACAA GCTTGAGATG
    GTTGTAACCA ACGGGAGAGG CGATGGGCCC CGCAGCGAAG TCAAGGAGTT CCCCACACCT
    GAAGGAGTGC CCAGCTCCCC CAGGTACTTA AGAATCCGAC AGCCAAATCT GGAAAGCATC
    AATTTGGAGT GGGATCACCC AGAACATCCC AATGGAGTCC TCACGGGATA CAACCTTAGA
    TATCAAGCCT TTAACGGATC CAAAACGGGC AGAACCCTGG TAGAGAACTT CTCTCCCAAC
    CAGACAAGGT TCACCGTGCA GAGGACAGAC CCCATCTCGC GCTATCGATT CTTCCTGCGT
    GCTCGGACAC AGGTGGGAGA TGGAGAAGTC ATAGTGGAAG AGTCACCAGC GCTCCTGAAT
    GAAGCCACGC CAACCCCAGC CTCCACCTGG TTGCCTCCAC CTACAACCGA GCTAACTCCA
    GCAGCCACCA TTGCCACCAC AACCACCACT GCCACCCCTA CTACTGAAAC CCCTCCTACT
    GAAATCCCTA CTACTGCCAT CCCTACCACC ACCACTACTA CAACCACCAC AGCTGCCAGC
    ACCGTCGCAA GCACTACAAC AACTGCAGAG AGGGCTGCGG CAGCCACCAC AAAACAGGAG
    CTGGCCACCA ATGGATCGTC CATATGGGAC ATAAGAGCTT GGGCTAATAG TAACTGGGCA
    AACATCACCT GGAGCCACAA TTACTCTGCA GGAACTGACT TTGTGGTTAA GTATATCACC
    AGTAACAAGA CAGAGAAATC TATCCCCGTT AAAGCTCAGA CCCCCTCCTC TGTGCAGCTG
    GCCAATCTGA CGCCGGGGAT GGTGTACAAG CTGTGGGTGT TCCCCATATG GTCTAGCCCC
    AGCGAGCACT CGTACATAAC CTTTACCACC AGCTCAGCTT ACACCAAGAA CCACGTGGAC
    ATCGCGACCC AGGGCTGGTT CATCGGGCTG ATGTGTGCCA TCGCCCTCCT GGTCCTCATT
    CTGCTGATTG TTTGCTTCAT TAAGAGGAGC AGAGGAGGGA AATACCCAGT GCGTGACAAC
    AAAGATGAGC ACCTGAATCC TGAAGACAAG AACGTAGAAG ATGGCTCATT CGACTACAGG
    TCTCTTGAAA GCGATGAAGA CAACAAACCA CTGCCCAACA GTCAGACCTC CCTGGATGGC
    ACGATAAAGC AACAGGAGAG TGACGACAGC TTGGTGGACT ACGGAGAGGG TGGGGAAGGG
    CAGTTCAACG AGGACGGCTC CTTCATTGGC CAGTACACAG TGAAAAAGGA CAAGGAGGAA
    ACGGAAGGCA ACGAGAGCTC AGAAGCCACG TCCCCAGTCA ATGCTATCTA CTCATTAGCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46518
    Clone ID Related Accession (Same CDS sequence) XM_015298911.2 , XM_015298911.1
    Accession Version XM_015298911.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA
    ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT
    GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT
    ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC
    ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT
    GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG
    AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA
    GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG
    CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG
    TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG
    GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT
    TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC
    CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC
    GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA
    CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG
    GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC
    GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154397.1
    CDS169..3939
    Translation

    Target ORF information:

    RefSeq Version XM_015298911.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298911.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA
    ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT
    GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT
    ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC
    ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT
    GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG
    AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA
    GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG
    CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG
    TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG
    GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT
    TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC
    CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC
    GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA
    CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG
    GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC
    GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46518
    Clone ID Related Accession (Same CDS sequence) XM_015298911.2 , XM_015298911.1
    Accession Version XM_015298911.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA
    ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT
    GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT
    ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC
    ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT
    GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG
    AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA
    GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG
    CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG
    TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG
    GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT
    TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC
    CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC
    GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA
    CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG
    GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC
    GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154397.1
    CDS159..3929
    Translation

    Target ORF information:

    RefSeq Version XM_015298911.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCCACGCCA
    ACCCCAGCCT CCACCTGGTT GCCTCCACCT ACAACCGAGC TAACTCCAGC AGCCACCATT
    GCCACCACAA CCACCACTGC CACCCCTACT ACTGAAACCC CTCCTACTGA AATCCCTACT
    ACTGCCATCC CTACCACCAC CACTACTACA ACCACCACAG CTGCCAGCAC CGTCGCAAGC
    ACTACAACAA CTGCAGAGAG GGCTGCGGCA GCCACCACAA AACAGGAGCT GGCCACCAAT
    GGATCGTCCA TATGGGACAT AAGAGCTTGG GCTAATAGTA ACTGGGCAAA CATCACCTGG
    AGCCACAATT ACTCTGCAGG AACTGACTTT GTGGTTAAGT ATATCACCAG TAACAAGACA
    GAGAAATCTA TCCCCGTTAA AGCTCAGACC CCCTCCTCTG TGCAGCTGGC CAATCTGACG
    CCGGGGATGG TGTACAAGCT GTGGGTGTTC CCCATATGGT CTAGCCCCAG CGAGCACTCG
    TACATAACCT TTACCACCAG CTCAGCTTAC ACCAAGAACC ACGTGGACAT CGCGACCCAG
    GGCTGGTTCA TCGGGCTGAT GTGTGCCATC GCCCTCCTGG TCCTCATTCT GCTGATTGTT
    TGCTTCATTA AGAGGAGCAG AGGAGGGAAA TACCCAGTGC GTGACAACAA AGATGAGCAC
    CTGAATCCTG AAGACAAGAA CGTAGAAGAT GGCTCATTCG ACTACAGGTC TCTTGAAAGC
    GATGAAGACA ACAAACCACT GCCCAACAGT CAGACCTCCC TGGATGGCAC GATAAAGCAA
    CAGGAGAGTG ACGACAGCTT GGTGGACTAC GGAGAGGGTG GGGAAGGGCA GTTCAACGAG
    GACGGCTCCT TCATTGGCCA GTACACAGTG AAAAAGGACA AGGAGGAAAC GGAAGGCAAC
    GAGAGCTCAG AAGCCACGTC CCCAGTCAAT GCTATCTACT CATTAGCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46519
    Clone ID Related Accession (Same CDS sequence) XM_015298912.1 , XM_015298912.2
    Accession Version XM_015298912.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC
    ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT
    AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC
    AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC
    GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT
    CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154398.1
    CDS159..3878
    Translation

    Target ORF information:

    RefSeq Version XM_015298912.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC
    ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT
    AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC
    AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC
    GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT
    CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46519
    Clone ID Related Accession (Same CDS sequence) XM_015298912.1 , XM_015298912.2
    Accession Version XM_015298912.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298912.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC
    ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT
    AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC
    AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC
    GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT
    CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154398.1
    CDS169..3888
    Translation

    Target ORF information:

    RefSeq Version XM_015298912.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298912.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC
    ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT
    AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC
    AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC
    GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT
    CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46520
    Clone ID Related Accession (Same CDS sequence) XM_015298913.2 , XM_015298913.1
    Accession Version XM_015298913.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154399.1
    CDS169..3762
    Translation

    Target ORF information:

    RefSeq Version XM_015298913.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298913.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46520
    Clone ID Related Accession (Same CDS sequence) XM_015298913.2 , XM_015298913.1
    Accession Version XM_015298913.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154399.1
    CDS159..3752
    Translation

    Target ORF information:

    RefSeq Version XM_015298913.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCT TACACCAAGA ACCACGTGGA CATCGCGACC
    CAGGGCTGGT TCATCGGGCT GATGTGTGCC ATCGCCCTCC TGGTCCTCAT TCTGCTGATT
    GTTTGCTTCA TTAAGAGGAG CAGAGGAGGG AAATACCCAG TGCGTGACAA CAAAGATGAG
    CACCTGAATC CTGAAGACAA GAACGTAGAA GATGGCTCAT TCGACTACAG GTCTCTTGAA
    AGCGATGAAG ACAACAAACC ACTGCCCAAC AGTCAGACCT CCCTGGATGG CACGATAAAG
    CAACAGGAGA GTGACGACAG CTTGGTGGAC TACGGAGAGG GTGGGGAAGG GCAGTTCAAC
    GAGGACGGCT CCTTCATTGG CCAGTACACA GTGAAAAAGG ACAAGGAGGA AACGGAAGGC
    AACGAGAGCT CAGAAGCCAC GTCCCCAGTC AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46521
    Clone ID Related Accession (Same CDS sequence) XM_015298914.1 , XM_015298914.2
    Accession Version XM_015298914.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC
    AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC
    GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC
    ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA
    GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154400.1
    CDS159..3737
    Translation

    Target ORF information:

    RefSeq Version XM_015298914.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC
    AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC
    GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC
    ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA
    GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46521
    Clone ID Related Accession (Same CDS sequence) XM_015298914.1 , XM_015298914.2
    Accession Version XM_015298914.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298914.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC
    AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC
    GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC
    ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA
    GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154400.1
    CDS169..3747
    Translation

    Target ORF information:

    RefSeq Version XM_015298914.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298914.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC
    AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC
    GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC
    ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA
    GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46522
    Clone ID Related Accession (Same CDS sequence) XM_015298915.1 , XM_015298915.2
    Accession Version XM_015298915.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC
    AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC
    TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA
    GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC
    AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154401.1
    CDS159..3722
    Translation

    Target ORF information:

    RefSeq Version XM_015298915.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC
    AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC
    TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA
    GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC
    AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46522
    Clone ID Related Accession (Same CDS sequence) XM_015298915.1 , XM_015298915.2
    Accession Version XM_015298915.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC
    AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC
    TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA
    GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC
    AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154401.1
    CDS169..3732
    Translation

    Target ORF information:

    RefSeq Version XM_015298915.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298915.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGCAA TCCCTGCTAA CCGTTTGAGA GACTTACCTA AAGAGCGACC CGACCGGCCC
    CGGGACCTGG AGCTGTCAGA CCTGGCTGAG AGGAGCGTGA AGCTGACATG GATTCCTGGC
    GATGACAACA ACAGCCCCAT CACAGACTAC ATCGTCCAGT TTGAGGAGGA CCGCTTCCAG
    CCTGGCACGT GGCACAACCA CTCCAGGTAT CCTGGGAATG TCAACTCGGC CCTCCTGAGC
    CTCTCTCCTT ACGTCAACTA CCAATTTCGG GTGATTGCAG TGAACGACGT GGGCAGCAGC
    CTGCCCAGCA TGCCCTCAGA ACGATACCAG ACCAGCGGGG CACGACCTGA AATTAACCCA
    ACAGGAGTTC AAGGAGCAGG GACCCAAAAA AACAACATGG AGATAACCTG GACGCCTCTG
    AATGCAACTC AAGCCTATGG GCCCAACCTC CGTTACATCG TGCGGTGGAG GCGAAGGGAC
    CCACGTGGGA GTTGGTACAA CGAGACGGTG AAGGCACCAC GACACGTCGT CTGGAACACA
    CCCATCTACG TCCCCTACGA GATCAAAGTG CAGGCAGAGA ATGACTTTGG TAGAGCTCCA
    GAGCCTGAGA CCTACATCGG CTACTCAGGG GAAGATTATC CCAAGGCTGC ACCTACAGAT
    GTTAGGATAA GAGTTTTAAA CAGCACTGCC ATTGCTCTGA CATGGACCCG CGTGCACCTG
    GACACCATCC AGGGACAGCT TAAGGAGTAC AGAGCCTATT TCTGGAGAGA CAGTAGTTTG
    CTGAAGAACC TGTGGGTCTC CAAAAAACGG CAGTATGTGA GTTTTCCTGG AGACCGAAAC
    CGGGGCATAG TGTCCCGGCT GTTCCCTTAC AGCAACTACA AGCTTGAGAT GGTTGTAACC
    AACGGGAGAG GCGATGGGCC CCGCAGCGAA GTCAAGGAGT TCCCCACACC TGAAGGAGTG
    CCCAGCTCCC CCAGGTACTT AAGAATCCGA CAGCCAAATC TGGAAAGCAT CAATTTGGAG
    TGGGATCACC CAGAACATCC CAATGGAGTC CTCACGGGAT ACAACCTTAG ATATCAAGCC
    TTTAACGGAT CCAAAACGGG CAGAACCCTG GTAGAGAACT TCTCTCCCAA CCAGACAAGG
    TTCACCGTGC AGAGGACAGA CCCCATCTCG CGCTATCGAT TCTTCCTGCG TGCTCGGACA
    CAGGTGGGAG ATGGAGAAGT CATAGTGGAA GAGTCACCAG CGCTCCTGAA TGAAGCCACG
    CCAACCCCAG CTTACACCAA GAACCACGTG GACATCGCGA CCCAGGGCTG GTTCATCGGG
    CTGATGTGTG CCATCGCCCT CCTGGTCCTC ATTCTGCTGA TTGTTTGCTT CATTAAGAGG
    AGCAGAGGAG GGAAATACCC AGTGCGTGAC AACAAAGATG AGCACCTGAA TCCTGAAGAC
    AAGAACGTAG AAGATGGCTC ATTCGACTAC AGCGATGAAG ACAACAAACC ACTGCCCAAC
    AGTCAGACCT CCCTGGATGG CACGATAAAG CAACAGGAGA GTGACGACAG CTTGGTGGAC
    TACGGAGAGG GTGGGGAAGG GCAGTTCAAC GAGGACGGCT CCTTCATTGG CCAGTACACA
    GTGAAAAAGG ACAAGGAGGA AACGGAAGGC AACGAGAGCT CAGAAGCCAC GTCCCCAGTC
    AATGCTATCT ACTCATTAGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46523
    Clone ID Related Accession (Same CDS sequence) XM_015298916.1 , XM_015298916.2
    Accession Version XM_015298916.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT
    GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG
    GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC
    GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG
    AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154402.1
    CDS159..3686
    Translation

    Target ORF information:

    RefSeq Version XM_015298916.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT
    GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG
    GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC
    GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG
    AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46523
    Clone ID Related Accession (Same CDS sequence) XM_015298916.1 , XM_015298916.2
    Accession Version XM_015298916.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT
    GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG
    GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC
    GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG
    AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154402.1
    CDS169..3696
    Translation

    Target ORF information:

    RefSeq Version XM_015298916.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298916.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT
    GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG
    GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC
    GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG
    AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46524
    Clone ID Related Accession (Same CDS sequence) XM_015298917.1 , XM_015298917.2
    Accession Version XM_015298917.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT
    GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC
    TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG
    TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT
    CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC
    CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA
    AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC
    CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG
    GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA
    GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA
    GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT
    GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG
    TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA
    CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT
    TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC
    GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC
    CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA
    GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC
    CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC
    TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG
    GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC
    GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC
    CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT
    GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC
    TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA
    AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC
    AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA
    GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154403.1
    CDS159..3635
    Translation

    Target ORF information:

    RefSeq Version XM_015298917.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT
    GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC
    TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG
    TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT
    CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC
    CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA
    AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC
    CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG
    GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA
    GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA
    GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT
    GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG
    TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA
    CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT
    TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC
    GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC
    CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA
    GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC
    CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC
    TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG
    GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC
    GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC
    CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT
    GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC
    TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA
    AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC
    AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA
    GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46524
    Clone ID Related Accession (Same CDS sequence) XM_015298917.1 , XM_015298917.2
    Accession Version XM_015298917.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT
    GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC
    TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG
    TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT
    CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC
    CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA
    AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC
    CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG
    GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA
    GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA
    GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT
    GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG
    TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA
    CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT
    TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC
    GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC
    CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA
    GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC
    CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC
    TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG
    GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC
    GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC
    CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT
    GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC
    TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA
    AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC
    AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA
    GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154403.1
    CDS169..3645
    Translation

    Target ORF information:

    RefSeq Version XM_015298917.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298917.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC ACCCGAGGGG TAACGGAAAC AACACCCAGC
    TTCATGTACC CATATGGGAC CTCCAGCAGC CAGATGGTGC TCCGAGGGGT GGACCTCTTG
    CTGGAGTGCA TTGCATCAGG AGTACCAGCA CCAGACATCA TGTGGTACAA GAAAGGAGGT
    GAGCTCCCAG CAGGCAAAAC CAAGCTGGAA AACTTTAACA AGGCCCTTCG TATCTCCAAC
    GTCTCGGAGG AAGACTCTGG GGAGTATTTC TGCCTGGCAT CCAACAAGAT GGGCAGCATC
    CGCCACACGA TCTCGGTGAG AGTGAAGGCT GCCCCGTATT GGCTGGATGA GCCACAGAAT
    CTCATTTTGG CCCCTGGTGA GGACGGCAGG TTGGTGTGTC GAGCCAATGG GAACCCCAAG
    CCTTCAATCC AGTGGTTGGT GAATGGAGAG CCCATTGAAG GTTCTCCACC CAACCCAAGC
    AGAGAGGTGG CTGGAGATAC CATTGTGTTT CGAGACACGC AGATCGGCAG CAGCGCTGTG
    TACCAATGCA ATGCTTCCAA CGAGCACGGC TACCTCCTTG CCAATGCCTT TGTCAGTGTC
    CTGGATGTGC CACCACGGAT ACTGGCCCCA CGCAACCAGC TCATCAAAGT CATCCAGTAC
    AACAGGACCC GGCTGGACTG CCCTTTTTTC GGCTCACCCA TCCCCACCCT GCGATGGTTT
    AAGAACGGCC AGGGGAACAT GCTGGATGGA GGGAACTACA AGGCGCATGA GAACGGGAGC
    TTGGAGATGA GCATGGCTCG GAAGGAGGAT CAGGGCATCT ACACCTGTGT CGCCACCAAC
    ATCCTGGGCA AAGTGGAGGC CCAGGTTCGC CTGGAAGTCA AAGACCCTAC CAGGATTGTG
    AGAGGCCCCG AAGATCAGGT GGTGAAGAGG GGCTCCATGC CTCGCCTGCA CTGCCGGGTG
    AAGCACGACC CAACACTGAA GCTCACGGTC ACCTGGCTGA AGGACGACGC TCCTCTCTAC
    ATTGGGAACA GGATGAAAAA AGAAGATGAT GGTCTGACAA TATATGGCGT GGCTGAGAAG
    GACCAGGGTG ACTACACCTG CGTGGCCAGC ACAGAGCTGG ACAAGGACTC AGCTAAAGCC
    TACCTCACCG TGCTAGAGCG ACCCGACCGG CCCCGGGACC TGGAGCTGTC AGACCTGGCT
    GAGAGGAGCG TGAAGCTGAC ATGGATTCCT GGCGATGACA ACAACAGCCC CATCACAGAC
    TACATCGTCC AGTTTGAGGA GGACCGCTTC CAGCCTGGCA CGTGGCACAA CCACTCCAGG
    TATCCTGGGA ATGTCAACTC GGCCCTCCTG AGCCTCTCTC CTTACGTCAA CTACCAATTT
    CGGGTGATTG CAGTGAACGA CGTGGGCAGC AGCCTGCCCA GCATGCCCTC AGAACGATAC
    CAGACCAGCG GGGCACGACC TGAAATTAAC CCAACAGGAG TTCAAGGAGC AGGGACCCAA
    AAAAACAACA TGGAGATAAC CTGGACGCCT CTGAATGCAA CTCAAGCCTA TGGGCCCAAC
    CTCCGTTACA TCGTGCGGTG GAGGCGAAGG GACCCACGTG GGAGTTGGTA CAACGAGACG
    GTGAAGGCAC CACGACACGT CGTCTGGAAC ACACCCATCT ACGTCCCCTA CGAGATCAAA
    GTGCAGGCAG AGAATGACTT TGGTAGAGCT CCAGAGCCTG AGACCTACAT CGGCTACTCA
    GGGGAAGATT ATCCCAAGGC TGCACCTACA GATGTTAGGA TAAGAGTTTT AAACAGCACT
    GCCATTGCTC TGACATGGAC CCGCGTGCAC CTGGACACCA TCCAGGGACA GCTTAAGGAG
    TACAGAGCCT ATTTCTGGAG AGACAGTAGT TTGCTGAAGA ACCTGTGGGT CTCCAAAAAA
    CGGCAGTATG TGAGTTTTCC TGGAGACCGA AACCGGGGCA TAGTGTCCCG GCTGTTCCCT
    TACAGCAACT ACAAGCTTGA GATGGTTGTA ACCAACGGGA GAGGCGATGG GCCCCGCAGC
    GAAGTCAAGG AGTTCCCCAC ACCTGAAGGA GTGCCCAGCT CCCCCAGGTA CTTAAGAATC
    CGACAGCCAA ATCTGGAAAG CATCAATTTG GAGTGGGATC ACCCAGAACA TCCCAATGGA
    GTCCTCACGG GATACAACCT TAGATATCAA GCCTTTAACG GATCCAAAAC GGGCAGAACC
    CTGGTAGAGA ACTTCTCTCC CAACCAGACA AGGTTCACCG TGCAGAGGAC AGACCCCATC
    TCGCGCTATC GATTCTTCCT GCGTGCTCGG ACACAGGTGG GAGATGGAGA AGTCATAGTG
    GAAGAGTCAC CAGCGCTCCT GAATGAAGCC ACGCCAACCC CAGCTTACAC CAAGAACCAC
    GTGGACATCG CGACCCAGGG CTGGTTCATC GGGCTGATGT GTGCCATCGC CCTCCTGGTC
    CTCATTCTGC TGATTGTTTG CTTCATTAAG AGGAGCAGAG GAGGGAAATA CCCAGTGCGT
    GACAACAAAG ATGAGCACCT GAATCCTGAA GACAAGAACG TAGAAGATGG CTCATTCGAC
    TACAGCGATG AAGACAACAA ACCACTGCCC AACAGTCAGA CCTCCCTGGA TGGCACGATA
    AAGCAACAGG AGAGTGACGA CAGCTTGGTG GACTACGGAG AGGGTGGGGA AGGGCAGTTC
    AACGAGGACG GCTCCTTCAT TGGCCAGTAC ACAGTGAAAA AGGACAAGGA GGAAACGGAA
    GGCAACGAGA GCTCAGAAGC CACGTCCCCA GTCAATGCTA TCTACTCATT AGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46525
    Clone ID Related Accession (Same CDS sequence) XM_015298918.1 , XM_015298918.2
    Accession Version XM_015298918.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC
    AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC
    CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC
    CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC
    TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT
    GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA
    GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG
    GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA
    GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154404.1
    CDS159..3404
    Translation

    Target ORF information:

    RefSeq Version XM_015298918.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC
    AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC
    CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC
    CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC
    TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT
    GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA
    GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG
    GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA
    GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46525
    Clone ID Related Accession (Same CDS sequence) XM_015298918.1 , XM_015298918.2
    Accession Version XM_015298918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC
    AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC
    CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC
    CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC
    TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT
    GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA
    GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG
    GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA
    GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154404.1
    CDS169..3414
    Translation

    Target ORF information:

    RefSeq Version XM_015298918.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTTGCCC
    AGCTCCCCCA GGTACTTAAG AATCCGACAG CCAAATCTGG AAAGCATCAA TTTGGAGTGG
    GATCACCCAG AACATCCCAA TGGAGTCCTC ACGGGATACA ACCTTAGATA TCAAGCCTTT
    AACGGATCCA AAACGGGCAG AACCCTGGTA GAGAACTTCT CTCCCAACCA GACAAGGTTC
    ACCGTGCAGA GGACAGACCC CATCTCGCGC TATCGATTCT TCCTGCGTGC TCGGACACAG
    GTGGGAGATG GAGAAGTCAT AGTGGAAGAG TCACCAGCGC TCCTGAATGA AGCTTACACC
    AAGAACCACG TGGACATCGC GACCCAGGGC TGGTTCATCG GGCTGATGTG TGCCATCGCC
    CTCCTGGTCC TCATTCTGCT GATTGTTTGC TTCATTAAGA GGAGCAGAGG AGGGAAATAC
    CCAGTGCGTG ACAACAAAGA TGAGCACCTG AATCCTGAAG ACAAGAACGT AGAAGATGGC
    TCATTCGACT ACAGCGATGA AGACAACAAA CCACTGCCCA ACAGTCAGAC CTCCCTGGAT
    GGCACGATAA AGCAACAGGA GAGTGACGAC AGCTTGGTGG ACTACGGAGA GGGTGGGGAA
    GGGCAGTTCA ACGAGGACGG CTCCTTCATT GGCCAGTACA CAGTGAAAAA GGACAAGGAG
    GAAACGGAAG GCAACGAGAG CTCAGAAGCC ACGTCCCCAG TCAATGCTAT CTACTCATTA
    GCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46527
    Clone ID Related Accession (Same CDS sequence) XM_015298920.1 , XM_015298920.2
    Accession Version XM_015298920.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456030.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154406.1
    CDS159..4187
    Translation

    Target ORF information:

    RefSeq Version XM_015298920.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46527
    Clone ID Related Accession (Same CDS sequence) XM_015298920.1 , XM_015298920.2
    Accession Version XM_015298920.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product neurofascin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154406.1
    CDS169..4197
    Translation

    Target ORF information:

    RefSeq Version XM_015298920.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298920.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTATCCCA AGGCTGCACC TACAGATGTT AGGATAAGAG TTTTAAACAG CACTGCCATT
    GCTCTGACAT GGACCCGCGT GCACCTGGAC ACCATCCAGG GACAGCTTAA GGAGTACAGA
    GCCTATTTCT GGAGAGACAG TAGTTTGCTG AAGAACCTGT GGGTCTCCAA AAAACGGCAG
    TATGTGAGTT TTCCTGGAGA CCGAAACCGG GGCATAGTGT CCCGGCTGTT CCCTTACAGC
    AACTACAAGC TTGAGATGGT TGTAACCAAC GGGAGAGGCG ATGGGCCCCG CAGCGAAGTC
    AAGGAGTTCC CCACACCTGA AGGAGTGCCC AGCTCCCCCA GGTACTTAAG AATCCGACAG
    CCAAATCTGG AAAGCATCAA TTTGGAGTGG GATCACCCAG AACATCCCAA TGGAGTCCTC
    ACGGGATACA ACCTTAGATA TCAAGCCTTT AACGGATCCA AAACGGGCAG AACCCTGGTA
    GAGAACTTCT CTCCCAACCA GACAAGGTTC ACCGTGCAGA GGACAGACCC CATCTCGCGC
    TATCGATTCT TCCTGCGTGC TCGGACACAG GTGGGAGATG GAGAAGTCAT AGTGGAAGAG
    TCACCAGCGC TCCTGAATGA AGCCACGCCA ACCCCAGCCT CCACCTGGTT GCCTCCACCT
    ACAACCGAGC TAACTCCAGC AGCCACCATT GCCACCACAA CCACCACTGC CACCCCTACT
    ACTGAAACCC CTCCTACTGA AATCCCTACT ACTGCCATCC CTACCACCAC CACTACTACA
    ACCACCACAG CTGCCAGCAC CGTCGCAAGC ACTACAACAA CTGCAGAGAG GGCTGCGGCA
    GCCACCACAA AACAGGAGCT GGCCACCAAT GGATCGTCCA TATGGGACAT AAGAGCTTGG
    GCTAATAGTA ACTGGGCAAA CATCACCTGG AGCCACAATT ACTCTGCAGG AACTGACTTT
    GTGGTTAAGT ATATCACCAG TAACAAGACA GAGAAATCTA TCCCCGTTAA AGCTCAGACC
    CCCTCCTCTG TGCAGCTGGC CAATCTGACG CCGGGGATGG TGTACAAGCT GTGGGTGTTC
    CCCATATGGT CTAGCCCCAG CGAGCACTCG TACATAACCT TTACCACCAG CTCAGCTTAC
    ACCAAGAACC ACGTGGACAT CGCGACCCAG GGCTGGTTCA TCGGGCTGAT GTGTGCCATC
    GCCCTCCTGG TCCTCATTCT GCTGATTGTT TGCTTCATTA AGAGGAGCAG AGGAGGGAAA
    TACCCAGTGC GTGACAACAA AGATGAGCAC CTGAATCCTG AAGACAAGAA CGTAGAAGAT
    GGCTCATTCG ACTACAGCGA TGAAGACAAC AAACCACTGC CCAACAGTCA GACCTCCCTG
    GATGGCACGA TAAAGCAACA GGAGAGTGAC GACAGCTTGG TGGACTACGG AGAGGGTGGG
    GAAGGGCAGT TCAACGAGGA CGGCTCCTTC ATTGGCCAGT ACACAGTGAA AAAGGACAAG
    GAGGAAACGG AAGGCAACGA GAGCTCAGAA GCCACGTCCC CAGTCAATGC TATCTACTCA
    TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28259
    Clone ID Related Accession (Same CDS sequence) NM_001160320.3
    Accession Version NM_001160320.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160320.2. Transcript Variant: This variant (4) lacks five alternate in-frame exons compared to variant 2. The resulting protein (isoform 4) lacks four internal segments, compared to isoform 2. This variant is the 2nd most abundant variant in E16 embryos and includes alternatively spliced exons 8, 22, and 23, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC TTACACCAAG AACCACGTGG ACATCGCGAC CCAGGGCTGG
    TTCATCGGGC TGATGTGTGC CATCGCCCTC CTGGTCCTCA TTCTGCTGAT TGTTTGCTTC
    ATTAAGAGGA GCAGAGGAGG GAAATACCCA GTGCGTGACA ACAAAGATGA GCACCTGAAT
    CCTGAAGACA AGAACGTAGA AGATGGCTCA TTCGACTACA GCGATGAAGA CAACAAACCA
    CTGCCCAACA GTCAGACCTC CCTGGATGGC ACGATAAAGC AACAGGAGAG TGACGACAGC
    TTGGTGGACT ACGGAGAGGG TGGGGAAGGG CAGTTCAACG AGGACGGCTC CTTCATTGGC
    CAGTACACAG TGAAAAAGGA CAAGGAGGAA ACGGAAGGCA ACGAGAGCTC AGAAGCCACG
    TCCCCAGTCA ATGCTATCTA CTCATTAGCG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153792.1
    CDS109..3621
    Translation

    Target ORF information:

    RefSeq Version NM_001160320.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160320.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC TTACACCAAG AACCACGTGG ACATCGCGAC CCAGGGCTGG
    TTCATCGGGC TGATGTGTGC CATCGCCCTC CTGGTCCTCA TTCTGCTGAT TGTTTGCTTC
    ATTAAGAGGA GCAGAGGAGG GAAATACCCA GTGCGTGACA ACAAAGATGA GCACCTGAAT
    CCTGAAGACA AGAACGTAGA AGATGGCTCA TTCGACTACA GCGATGAAGA CAACAAACCA
    CTGCCCAACA GTCAGACCTC CCTGGATGGC ACGATAAAGC AACAGGAGAG TGACGACAGC
    TTGGTGGACT ACGGAGAGGG TGGGGAAGGG CAGTTCAACG AGGACGGCTC CTTCATTGGC
    CAGTACACAG TGAAAAAGGA CAAGGAGGAA ACGGAAGGCA ACGAGAGCTC AGAAGCCACG
    TCCCCAGTCA ATGCTATCTA CTCATTAGCG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28267
    Clone ID Related Accession (Same CDS sequence) NM_001160322.3
    Accession Version NM_001160322.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 6 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160322.2. Transcript Variant: This variant (6) lacks four alternate in-frame exons and includes two alternate in-frame exons, compared to variant 2. The resulting protein (isoform 6) lacks three internal segments and includes an additional segment, compared to isoform 2. This variant is the 4th most abundant variant in E16 embryos and includes alternatively spliced exons 3, 17, 26, 27, 28, and 29, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC
    ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT
    AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC
    AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC
    GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT
    GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG
    GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC
    GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG
    AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153794.1
    CDS109..3816
    Translation

    Target ORF information:

    RefSeq Version NM_001160322.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160322.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCCACCAATG GATCGTCCAT ATGGGACATA AGAGCTTGGG CTAATAGTAA CTGGGCAAAC
    ATCACCTGGA GCCACAATTA CTCTGCAGGA ACTGACTTTG TGGTTAAGTA TATCACCAGT
    AACAAGACAG AGAAATCTAT CCCCGTTAAA GCTCAGACCC CCTCCTCTGT GCAGCTGGCC
    AATCTGACGC CGGGGATGGT GTACAAGCTG TGGGTGTTCC CCATATGGTC TAGCCCCAGC
    GAGCACTCGT ACATAACCTT TACCACCAGC TCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGCGAT
    GAAGACAACA AACCACTGCC CAACAGTCAG ACCTCCCTGG ATGGCACGAT AAAGCAACAG
    GAGAGTGACG ACAGCTTGGT GGACTACGGA GAGGGTGGGG AAGGGCAGTT CAACGAGGAC
    GGCTCCTTCA TTGGCCAGTA CACAGTGAAA AAGGACAAGG AGGAAACGGA AGGCAACGAG
    AGCTCAGAAG CCACGTCCCC AGTCAATGCT ATCTACTCAT TAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28270
    Clone ID Related Accession (Same CDS sequence) NM_001004493.4
    Accession Version NM_001004493.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001004493.3. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: X65224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC
    AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC
    GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC
    ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA
    GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001004493.1
    CDS109..3927
    Translation

    Target ORF information:

    RefSeq Version NM_001004493.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001004493.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAA GAAACCCCAT
    AACGAAACGT CCTTACGAAA TCACACTGAC ATGTACAGTG CCCGAGGGGT AACGGAAACA
    ACACCCAGCT TCATGTACCC ATATGGGACC TCCAGCAGCC AGATGGTGCT CCGAGGGGTG
    GACCTCTTGC TGGAGTGCAT TGCATCAGGA GTACCAGCAC CAGACATCAT GTGGTACAAG
    AAAGGAGGTG AGCTCCCAGC AGGCAAAACC AAGCTGGAAA ACTTTAACAA GGCCCTTCGT
    ATCTCCAACG TCTCGGAGGA AGACTCTGGG GAGTATTTCT GCCTGGCATC CAACAAGATG
    GGCAGCATCC GCCACACGAT CTCGGTGAGA GTGAAGGCTG CCCCGTATTG GCTGGATGAG
    CCACAGAATC TCATTTTGGC CCCTGGTGAG GACGGCAGGT TGGTGTGTCG AGCCAATGGG
    AACCCCAAGC CTTCAATCCA GTGGTTGGTG AATGGAGAGC CCATTGAAGG TTCTCCACCC
    AACCCAAGCA GAGAGGTGGC TGGAGATACC ATTGTGTTTC GAGACACGCA GATCGGCAGC
    AGCGCTGTGT ACCAATGCAA TGCTTCCAAC GAGCACGGCT ACCTCCTTGC CAATGCCTTT
    GTCAGTGTCC TGGATGTGCC ACCACGGATA CTGGCCCCAC GCAACCAGCT CATCAAAGTC
    ATCCAGTACA ACAGGACCCG GCTGGACTGC CCTTTTTTCG GCTCACCCAT CCCCACCCTG
    CGATGGTTTA AGAACGGCCA GGGGAACATG CTGGATGGAG GGAACTACAA GGCGCATGAG
    AACGGGAGCT TGGAGATGAG CATGGCTCGG AAGGAGGATC AGGGCATCTA CACCTGTGTC
    GCCACCAACA TCCTGGGCAA AGTGGAGGCC CAGGTTCGCC TGGAAGTCAA AGACCCTACC
    AGGATTGTGA GAGGCCCCGA AGATCAGGTG GTGAAGAGGG GCTCCATGCC TCGCCTGCAC
    TGCCGGGTGA AGCACGACCC AACACTGAAG CTCACGGTCA CCTGGCTGAA GGACGACGCT
    CCTCTCTACA TTGGGAACAG GATGAAAAAA GAAGATGATG GTCTGACAAT ATATGGCGTG
    GCTGAGAAGG ACCAGGGTGA CTACACCTGC GTGGCCAGCA CAGAGCTGGA CAAGGACTCA
    GCTAAAGCCT ACCTCACCGT GCTAGCAATC CCTGCTAACC GTTTGAGAGA CTTACCTAAA
    GAGCGACCCG ACCGGCCCCG GGACCTGGAG CTGTCAGACC TGGCTGAGAG GAGCGTGAAG
    CTGACATGGA TTCCTGGCGA TGACAACAAC AGCCCCATCA CAGACTACAT CGTCCAGTTT
    GAGGAGGACC GCTTCCAGCC TGGCACGTGG CACAACCACT CCAGGTATCC TGGGAATGTC
    AACTCGGCCC TCCTGAGCCT CTCTCCTTAC GTCAACTACC AATTTCGGGT GATTGCAGTG
    AACGACGTGG GCAGCAGCCT GCCCAGCATG CCCTCAGAAC GATACCAGAC CAGCGGGGCA
    CGACCTGAAA TTAACCCAAC AGGAGTTCAA GGAGCAGGGA CCCAAAAAAA CAACATGGAG
    ATAACCTGGA CGCCTCTGAA TGCAACTCAA GCCTATGGGC CCAACCTCCG TTACATCGTG
    CGGTGGAGGC GAAGGGACCC ACGTGGGAGT TGGTACAACG AGACGGTGAA GGCACCACGA
    CACGTCGTCT GGAACACACC CATCTACGTC CCCTACGAGA TCAAAGTGCA GGCAGAGAAT
    GACTTTGGTA GAGCTCCAGA GCCTGAGACC TACATCGGCT ACTCAGGGGA AGATTATCCC
    AAGGCTGCAC CTACAGATGT TAGGATAAGA GTTTTAAACA GCACTGCCAT TGCTCTGACA
    TGGACCCGCG TGCACCTGGA CACCATCCAG GGACAGCTTA AGGAGTACAG AGCCTATTTC
    TGGAGAGACA GTAGTTTGCT GAAGAACCTG TGGGTCTCCA AAAAACGGCA GTATGTGAGT
    TTTCCTGGAG ACCGAAACCG GGGCATAGTG TCCCGGCTGT TCCCTTACAG CAACTACAAG
    CTTGAGATGG TTGTAACCAA CGGGAGAGGC GATGGGCCCC GCAGCGAAGT CAAGGAGTTC
    CCCACACCTG AAGGAGTGCC CAGCTCCCCC AGGTACTTAA GAATCCGACA GCCAAATCTG
    GAAAGCATCA ATTTGGAGTG GGATCACCCA GAACATCCCA ATGGAGTCCT CACGGGATAC
    AACCTTAGAT ATCAAGCCTT TAACGGATCC AAAACGGGCA GAACCCTGGT AGAGAACTTC
    TCTCCCAACC AGACAAGGTT CACCGTGCAG AGGACAGACC CCATCTCGCG CTATCGATTC
    TTCCTGCGTG CTCGGACACA GGTGGGAGAT GGAGAAGTCA TAGTGGAAGA GTCACCAGCG
    CTCCTGAATG AAGCCACGCC AACCCCAGCC TCCACCTGGT TGCCTCCACC TACAACCGAG
    CTAACTCCAG CAGCCACCAT TGCCACCACA ACCACCACTG CCACCCCTAC TACTGAAACC
    CCTCCTACTG AAATCCCTAC TACTGCCATC CCTACCACCA CCACTACTAC AACCACCACA
    GCTGCCAGCA CCGTCGCAAG CACTACAACA ACTGCAGAGA GGGCTGCGGC AGCCACCACA
    AAACAGGAGC TGGCTTACAC CAAGAACCAC GTGGACATCG CGACCCAGGG CTGGTTCATC
    GGGCTGATGT GTGCCATCGC CCTCCTGGTC CTCATTCTGC TGATTGTTTG CTTCATTAAG
    AGGAGCAGAG GAGGGAAATA CCCAGTGCGT GACAACAAAG ATGAGCACCT GAATCCTGAA
    GACAAGAACG TAGAAGATGG CTCATTCGAC TACAGGTCTC TTGAAAGCGA TGAAGACAAC
    AAACCACTGC CCAACAGTCA GACCTCCCTG GATGGCACGA TAAAGCAACA GGAGAGTGAC
    GACAGCTTGG TGGACTACGG AGAGGGTGGG GAAGGGCAGT TCAACGAGGA CGGCTCCTTC
    ATTGGCCAGT ACACAGTGAA AAAGGACAAG GAGGAAACGG AAGGCAACGA GAGCTCAGAA
    GCCACGTCCC CAGTCAATGC TATCTACTCA TTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28275
    Clone ID Related Accession (Same CDS sequence) NM_001160321.3
    Accession Version NM_001160321.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 5 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160321.2. Transcript Variant: This variant (5) lacks five alternate in-frame exons compared to variant 2. The resulting protein (isoform 5) lacks four internal segments, compared to isoform 2. This variant is the 3rd most abundant variant in E16 embryos and includes alternatively spliced exons 3, 17, and 26, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCTTA CACCAAGAAC CACGTGGACA TCGCGACCCA GGGCTGGTTC
    ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG GTCCTCATTC TGCTGATTGT TTGCTTCATT
    AAGAGGAGCA GAGGAGGGAA ATACCCAGTG CGTGACAACA AAGATGAGCA CCTGAATCCT
    GAAGACAAGA ACGTAGAAGA TGGCTCATTC GACTACAGCG ATGAAGACAA CAAACCACTG
    CCCAACAGTC AGACCTCCCT GGATGGCACG ATAAAGCAAC AGGAGAGTGA CGACAGCTTG
    GTGGACTACG GAGAGGGTGG GGAAGGGCAG TTCAACGAGG ACGGCTCCTT CATTGGCCAG
    TACACAGTGA AAAAGGACAA GGAGGAAACG GAAGGCAACG AGAGCTCAGA AGCCACGTCC
    CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153793.1
    CDS109..3318
    Translation

    Target ORF information:

    RefSeq Version NM_001160321.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160321.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCTTA CACCAAGAAC CACGTGGACA TCGCGACCCA GGGCTGGTTC
    ATCGGGCTGA TGTGTGCCAT CGCCCTCCTG GTCCTCATTC TGCTGATTGT TTGCTTCATT
    AAGAGGAGCA GAGGAGGGAA ATACCCAGTG CGTGACAACA AAGATGAGCA CCTGAATCCT
    GAAGACAAGA ACGTAGAAGA TGGCTCATTC GACTACAGCG ATGAAGACAA CAAACCACTG
    CCCAACAGTC AGACCTCCCT GGATGGCACG ATAAAGCAAC AGGAGAGTGA CGACAGCTTG
    GTGGACTACG GAGAGGGTGG GGAAGGGCAG TTCAACGAGG ACGGCTCCTT CATTGGCCAG
    TACACAGTGA AAAAGGACAA GGAGGAAACG GAAGGCAACG AGAGCTCAGA AGCCACGTCC
    CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28276
    Clone ID Related Accession (Same CDS sequence) NM_001160323.3
    Accession Version NM_001160323.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 7 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001160323.2. Transcript Variant: This variant (7) lacks three alternate in-frame exons compared to variant 2. The resulting protein (isoform 7) lacks three internal segments, compared to isoform 2. This variant is the 5th most abundant variant in E16 embryos and includes alternatively spliced exons 8, 22, 23, 26, and 32, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT
    CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153795.1
    CDS109..3648
    Translation

    Target ORF information:

    RefSeq Version NM_001160323.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160323.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTTGCCTC AGCCCCCCAC CATCACCAAG
    CAGTCTGTGA AGGACTACAT CGTTGACCCC CGGGACAACA TCTTCATTGA ATGTGAAGCC
    AAAGGGAATC CTGTTCCCAC CTTTTCCTGG ACACGGAATG GGAAGTTCTT CAACGTGGCA
    AAGGACCCCA AAGTGTCCAT GCGGAGGCGG TCGGGGACGT TGGTCATCGA TTTCCATGGG
    GGTGGGCGGC CGGATGACTA CGAGGGCGAG TACCAATGCT TCGCCCGCAA CGATTATGGC
    ACTGCACTGT CCAGCAAAAT CCACCTCCAG GTGTCCAGAT CTCCCCTGTG GCCCAAGGAG
    AAGGTGGATG TCATTGAGGT TGACGAAGGT GCTCCGCTCA GCCTGCAGTG CAACCCGCCT
    CCTGGTCTGC CTCCTCCTGT CATCTTCTGG ATGAGCAGCT CCATGGAGCC CATCCACCAG
    GACAAGCGTG TCTCCCAGGG CCAGAATGGT GATCTGTACT TCTCCAATGT CATGCTGCAG
    GATGCCCAGA CTGATTACAG CTGCAATGCA CGCTTCCACT TCACCCACAC CATCCAGCAG
    AAAAATCCCT ACACCCTCAA GGTGAAAACC AAGAAACCCC ATAACGAAAC GTCCTTACGA
    AATCACACTG ACATGTACAG TGCCCGAGGG GTAACGGAAA CAACACCCAG CTTCATGTAC
    CCATATGGGA CCTCCAGCAG CCAGATGGTG CTCCGAGGGG TGGACCTCTT GCTGGAGTGC
    ATTGCATCAG GAGTACCAGC ACCAGACATC ATGTGGTACA AGAAAGGAGG TGAGCTCCCA
    GCAGGCAAAA CCAAGCTGGA AAACTTTAAC AAGGCCCTTC GTATCTCCAA CGTCTCGGAG
    GAAGACTCTG GGGAGTATTT CTGCCTGGCA TCCAACAAGA TGGGCAGCAT CCGCCACACG
    ATCTCGGTGA GAGTGAAGGC TGCCCCGTAT TGGCTGGATG AGCCACAGAA TCTCATTTTG
    GCCCCTGGTG AGGACGGCAG GTTGGTGTGT CGAGCCAATG GGAACCCCAA GCCTTCAATC
    CAGTGGTTGG TGAATGGAGA GCCCATTGAA GGTTCTCCAC CCAACCCAAG CAGAGAGGTG
    GCTGGAGATA CCATTGTGTT TCGAGACACG CAGATCGGCA GCAGCGCTGT GTACCAATGC
    AATGCTTCCA ACGAGCACGG CTACCTCCTT GCCAATGCCT TTGTCAGTGT CCTGGATGTG
    CCACCACGGA TACTGGCCCC ACGCAACCAG CTCATCAAAG TCATCCAGTA CAACAGGACC
    CGGCTGGACT GCCCTTTTTT CGGCTCACCC ATCCCCACCC TGCGATGGTT TAAGAACGGC
    CAGGGGAACA TGCTGGATGG AGGGAACTAC AAGGCGCATG AGAACGGGAG CTTGGAGATG
    AGCATGGCTC GGAAGGAGGA TCAGGGCATC TACACCTGTG TCGCCACCAA CATCCTGGGC
    AAAGTGGAGG CCCAGGTTCG CCTGGAAGTC AAAGACCCTA CCAGGATTGT GAGAGGCCCC
    GAAGATCAGG TGGTGAAGAG GGGCTCCATG CCTCGCCTGC ACTGCCGGGT GAAGCACGAC
    CCAACACTGA AGCTCACGGT CACCTGGCTG AAGGACGACG CTCCTCTCTA CATTGGGAAC
    AGGATGAAAA AAGAAGATGA TGGTCTGACA ATATATGGCG TGGCTGAGAA GGACCAGGGT
    GACTACACCT GCGTGGCCAG CACAGAGCTG GACAAGGACT CAGCTAAAGC CTACCTCACC
    GTGCTAGAGC GACCCGACCG GCCCCGGGAC CTGGAGCTGT CAGACCTGGC TGAGAGGAGC
    GTGAAGCTGA CATGGATTCC TGGCGATGAC AACAACAGCC CCATCACAGA CTACATCGTC
    CAGTTTGAGG AGGACCGCTT CCAGCCTGGC ACGTGGCACA ACCACTCCAG GTATCCTGGG
    AATGTCAACT CGGCCCTCCT GAGCCTCTCT CCTTACGTCA ACTACCAATT TCGGGTGATT
    GCAGTGAACG ACGTGGGCAG CAGCCTGCCC AGCATGCCCT CAGAACGATA CCAGACCAGC
    GGGGCACGAC CTGAAATTAA CCCAACAGGA GTTCAAGGAG CAGGGACCCA AAAAAACAAC
    ATGGAGATAA CCTGGACGCC TCTGAATGCA ACTCAAGCCT ATGGGCCCAA CCTCCGTTAC
    ATCGTGCGGT GGAGGCGAAG GGACCCACGT GGGAGTTGGT ACAACGAGAC GGTGAAGGCA
    CCACGACACG TCGTCTGGAA CACACCCATC TACGTCCCCT ACGAGATCAA AGTGCAGGCA
    GAGAATGACT TTGGTAGAGC TCCAGAGCCT GAGACCTACA TCGGCTACTC AGGGGAAGAT
    TATCCCAAGG CTGCACCTAC AGATGTTAGG ATAAGAGTTT TAAACAGCAC TGCCATTGCT
    CTGACATGGA CCCGCGTGCA CCTGGACACC ATCCAGGGAC AGCTTAAGGA GTACAGAGCC
    TATTTCTGGA GAGACAGTAG TTTGCTGAAG AACCTGTGGG TCTCCAAAAA ACGGCAGTAT
    GTGAGTTTTC CTGGAGACCG AAACCGGGGC ATAGTGTCCC GGCTGTTCCC TTACAGCAAC
    TACAAGCTTG AGATGGTTGT AACCAACGGG AGAGGCGATG GGCCCCGCAG CGAAGTCAAG
    GAGTTCCCCA CACCTGAAGG AGTGCCCAGC TCCCCCAGGT ACTTAAGAAT CCGACAGCCA
    AATCTGGAAA GCATCAATTT GGAGTGGGAT CACCCAGAAC ATCCCAATGG AGTCCTCACG
    GGATACAACC TTAGATATCA AGCCTTTAAC GGATCCAAAA CGGGCAGAAC CCTGGTAGAG
    AACTTCTCTC CCAACCAGAC AAGGTTCACC GTGCAGAGGA CAGACCCCAT CTCGCGCTAT
    CGATTCTTCC TGCGTGCTCG GACACAGGTG GGAGATGGAG AAGTCATAGT GGAAGAGTCA
    CCAGCGCTCC TGAATGAAGC CACGCCAACC CCAGCTTACA CCAAGAACCA CGTGGACATC
    GCGACCCAGG GCTGGTTCAT CGGGCTGATG TGTGCCATCG CCCTCCTGGT CCTCATTCTG
    CTGATTGTTT GCTTCATTAA GAGGAGCAGA GGAGGGAAAT ACCCAGTGCG TGACAACAAA
    GATGAGCACC TGAATCCTGA AGACAAGAAC GTAGAAGATG GCTCATTCGA CTACAGGTCT
    CTTGAAAGCG ATGAAGACAA CAAACCACTG CCCAACAGTC AGACCTCCCT GGATGGCACG
    ATAAAGCAAC AGGAGAGTGA CGACAGCTTG GTGGACTACG GAGAGGGTGG GGAAGGGCAG
    TTCAACGAGG ACGGCTCCTT CATTGGCCAG TACACAGTGA AAAAGGACAA GGAGGAAACG
    GAAGGCAACG AGAGCTCAGA AGCCACGTCC CCAGTCAATG CTATCTACTC ATTAGCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28282
    Clone ID Related Accession (Same CDS sequence) NM_001160324.3
    Accession Version NM_001160324.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 8 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 10, 2016 this sequence version replaced NM_001160324.2. Transcript Variant: This variant (8) lacks six alternate in-frame exons compared to variant 2. The resulting protein (isoform 8) lacks four internal segments, compared to isoform 2. This variant is the most abundant variant in E06 embryos and includes alternatively spliced exons 3 and 17, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT
    GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA
    GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA
    GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC
    TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG
    GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG
    GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC
    TACTCATTAG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153796.1
    CDS109..3303
    Translation

    Target ORF information:

    RefSeq Version NM_001160324.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160324.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT
    GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA
    GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA
    GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC
    TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG
    GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG
    GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC
    TACTCATTAG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28289
    Clone ID Related Accession (Same CDS sequence) NM_001004709.4
    Accession Version NM_001004709.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Gallus gallus(chicken)
    Product neurofascin isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000168.1. On Feb 11, 2016 this sequence version replaced NM_001004709.3. Transcript Variant: This variant (3) lacks four alternate in-frame exons compared to variant 2. The resulting protein (isoform 3) lacks three internal segments, compared to isoform 2. This variant is the most abundant variant in E16 embryos and includes alternatively spliced exons 3, 17, 26, and 27, as defined by Hassel et al (PMID: 9353344). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on data from Hassel et al (PMID: 9353344). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT
    GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA
    GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA
    GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC
    TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG
    GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG
    GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC
    TACTCATTAG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001004709.2
    CDS109..3543
    Translation

    Target ORF information:

    RefSeq Version NM_001004709.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neurofascin (NFASC), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001004709.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTCCTGC ACAGCCACCA GCTCACCTAC GCGGGGATCG CATTCGCTCT GTGCCTCCAC 
    CACCTCATCA GCGCCATTGA AGTCCCTCTG GATTCAAATA TTCAGAGTGA ATTGCCTCAG
    CCCCCCACCA TCACCAAGCA GTCTGTGAAG GACTACATCG TTGACCCCCG GGACAACATC
    TTCATTGAAT GTGAAGCCAA AGGGAATCCT GTTCCCACCT TTTCCTGGAC ACGGAATGGG
    AAGTTCTTCA ACGTGGCAAA GGACCCCAAA GTGTCCATGC GGAGGCGGTC GGGGACGTTG
    GTCATCGATT TCCATGGGGG TGGGCGGCCG GATGACTACG AGGGCGAGTA CCAATGCTTC
    GCCCGCAACG ATTATGGCAC TGCACTGTCC AGCAAAATCC ACCTCCAGGT GTCCAGATCT
    CCCCTGTGGC CCAAGGAGAA GGTGGATGTC ATTGAGGTTG ACGAAGGTGC TCCGCTCAGC
    CTGCAGTGCA ACCCGCCTCC TGGTCTGCCT CCTCCTGTCA TCTTCTGGAT GAGCAGCTCC
    ATGGAGCCCA TCCACCAGGA CAAGCGTGTC TCCCAGGGCC AGAATGGTGA TCTGTACTTC
    TCCAATGTCA TGCTGCAGGA TGCCCAGACT GATTACAGCT GCAATGCACG CTTCCACTTC
    ACCCACACCA TCCAGCAGAA AAATCCCTAC ACCCTCAAGG TGAAAACCAC CCGAGGGGTA
    ACGGAAACAA CACCCAGCTT CATGTACCCA TATGGGACCT CCAGCAGCCA GATGGTGCTC
    CGAGGGGTGG ACCTCTTGCT GGAGTGCATT GCATCAGGAG TACCAGCACC AGACATCATG
    TGGTACAAGA AAGGAGGTGA GCTCCCAGCA GGCAAAACCA AGCTGGAAAA CTTTAACAAG
    GCCCTTCGTA TCTCCAACGT CTCGGAGGAA GACTCTGGGG AGTATTTCTG CCTGGCATCC
    AACAAGATGG GCAGCATCCG CCACACGATC TCGGTGAGAG TGAAGGCTGC CCCGTATTGG
    CTGGATGAGC CACAGAATCT CATTTTGGCC CCTGGTGAGG ACGGCAGGTT GGTGTGTCGA
    GCCAATGGGA ACCCCAAGCC TTCAATCCAG TGGTTGGTGA ATGGAGAGCC CATTGAAGGT
    TCTCCACCCA ACCCAAGCAG AGAGGTGGCT GGAGATACCA TTGTGTTTCG AGACACGCAG
    ATCGGCAGCA GCGCTGTGTA CCAATGCAAT GCTTCCAACG AGCACGGCTA CCTCCTTGCC
    AATGCCTTTG TCAGTGTCCT GGATGTGCCA CCACGGATAC TGGCCCCACG CAACCAGCTC
    ATCAAAGTCA TCCAGTACAA CAGGACCCGG CTGGACTGCC CTTTTTTCGG CTCACCCATC
    CCCACCCTGC GATGGTTTAA GAACGGCCAG GGGAACATGC TGGATGGAGG GAACTACAAG
    GCGCATGAGA ACGGGAGCTT GGAGATGAGC ATGGCTCGGA AGGAGGATCA GGGCATCTAC
    ACCTGTGTCG CCACCAACAT CCTGGGCAAA GTGGAGGCCC AGGTTCGCCT GGAAGTCAAA
    GACCCTACCA GGATTGTGAG AGGCCCCGAA GATCAGGTGG TGAAGAGGGG CTCCATGCCT
    CGCCTGCACT GCCGGGTGAA GCACGACCCA ACACTGAAGC TCACGGTCAC CTGGCTGAAG
    GACGACGCTC CTCTCTACAT TGGGAACAGG ATGAAAAAAG AAGATGATGG TCTGACAATA
    TATGGCGTGG CTGAGAAGGA CCAGGGTGAC TACACCTGCG TGGCCAGCAC AGAGCTGGAC
    AAGGACTCAG CTAAAGCCTA CCTCACCGTG CTAGCAATCC CTGCTAACCG TTTGAGAGAC
    TTACCTAAAG AGCGACCCGA CCGGCCCCGG GACCTGGAGC TGTCAGACCT GGCTGAGAGG
    AGCGTGAAGC TGACATGGAT TCCTGGCGAT GACAACAACA GCCCCATCAC AGACTACATC
    GTCCAGTTTG AGGAGGACCG CTTCCAGCCT GGCACGTGGC ACAACCACTC CAGGTATCCT
    GGGAATGTCA ACTCGGCCCT CCTGAGCCTC TCTCCTTACG TCAACTACCA ATTTCGGGTG
    ATTGCAGTGA ACGACGTGGG CAGCAGCCTG CCCAGCATGC CCTCAGAACG ATACCAGACC
    AGCGGGGCAC GACCTGAAAT TAACCCAACA GGAGTTCAAG GAGCAGGGAC CCAAAAAAAC
    AACATGGAGA TAACCTGGAC GCCTCTGAAT GCAACTCAAG CCTATGGGCC CAACCTCCGT
    TACATCGTGC GGTGGAGGCG AAGGGACCCA CGTGGGAGTT GGTACAACGA GACGGTGAAG
    GCACCACGAC ACGTCGTCTG GAACACACCC ATCTACGTCC CCTACGAGAT CAAAGTGCAG
    GCAGAGAATG ACTTTGGTAG AGCTCCAGAG CCTGAGACCT ACATCGGCTA CTCAGGGGAA
    GATTTGCCCA GCTCCCCCAG GTACTTAAGA ATCCGACAGC CAAATCTGGA AAGCATCAAT
    TTGGAGTGGG ATCACCCAGA ACATCCCAAT GGAGTCCTCA CGGGATACAA CCTTAGATAT
    CAAGCCTTTA ACGGATCCAA AACGGGCAGA ACCCTGGTAG AGAACTTCTC TCCCAACCAG
    ACAAGGTTCA CCGTGCAGAG GACAGACCCC ATCTCGCGCT ATCGATTCTT CCTGCGTGCT
    CGGACACAGG TGGGAGATGG AGAAGTCATA GTGGAAGAGT CACCAGCGCT CCTGAATGAA
    GCCACGCCAA CCCCAGCCTC CACCTGGTTG CCTCCACCTA CAACCGAGCT AACTCCAGCA
    GCCACCATTG CCACCACAAC CACCACTGCC ACCCCTACTA CTGAAACCCC TCCTACTGAA
    ATCCCTACTA CTGCCATCCC TACCACCACC ACTACTACAA CCACCACAGC TGCCAGCACC
    GTCGCAAGCA CTACAACAAC TGCAGAGAGG GCTGCGGCAG CCACCACAAA ACAGGAGCTG
    GCTTACACCA AGAACCACGT GGACATCGCG ACCCAGGGCT GGTTCATCGG GCTGATGTGT
    GCCATCGCCC TCCTGGTCCT CATTCTGCTG ATTGTTTGCT TCATTAAGAG GAGCAGAGGA
    GGGAAATACC CAGTGCGTGA CAACAAAGAT GAGCACCTGA ATCCTGAAGA CAAGAACGTA
    GAAGATGGCT CATTCGACTA CAGCGATGAA GACAACAAAC CACTGCCCAA CAGTCAGACC
    TCCCTGGATG GCACGATAAA GCAACAGGAG AGTGACGACA GCTTGGTGGA CTACGGAGAG
    GGTGGGGAAG GGCAGTTCAA CGAGGACGGC TCCTTCATTG GCCAGTACAC AGTGAAAAAG
    GACAAGGAGG AAACGGAAGG CAACGAGAGC TCAGAAGCCA CGTCCCCAGT CAATGCTATC
    TACTCATTAG CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.