PUM1 cDNA ORF clone, Gallus gallus(chicken)

The following PUM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa147399 XM_025142980.1
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa147397 XM_025142978.1
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa147398 XM_025142979.1
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa45495 XM_015297531.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45496 XM_015297532.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45497 XM_015297533.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45498 XM_015297534.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45499 XM_015297535.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45500 XM_015297537.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45501 XM_015297538.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45502 XM_015297539.1
Latest version!
Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa45503 XM_015297540.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa45504 XM_015297541.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa45505 XM_015297542.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa00041 NM_001012840.2
Latest version!
Gallus gallus pumilio RNA binding family member 1 (PUM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa45496 XM_015297532.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45500 XM_015297537.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45499 XM_015297535.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45501 XM_015297538.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45495 XM_015297531.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45503 XM_015297540.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa45497 XM_015297533.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45504 XM_015297541.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa45498 XM_015297534.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa45505 XM_015297542.1 Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa147399
    Clone ID Related Accession (Same CDS sequence) XM_025142980.1
    Accession Version XM_025142980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024998748.1
    CDS117..3038
    Translation

    Target ORF information:

    RefSeq Version XM_025142980.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147397
    Clone ID Related Accession (Same CDS sequence) XM_025142978.1
    Accession Version XM_025142978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGGGGCCAAG GGATGCAGAG AATGATGAGA ATGACAAAGG GGATAAGAAA
    AATAAGGGCA CATTTGATGG CGATAAATTA GGAGATCTGA AGGAGGAGGG GGATGTGATG
    GATAAAACAA ATGGTTTGCC TGTGCAGAAT GGAATCGACA CAGATGTCAA AGACTTCAGC
    CGTACACCTG GTAATTGCCA GAACTCTGCT AGTGAAGTTG ATCTTCTGGG TCCAAACCAG
    AATGGATCTG AGGGCTTAGC CCAGCTGGCG AGTACTAATG GTGCCAAGCC CGTGGAGGAT
    TTTTCCAACA TAGAGTCACA GAGTGTTCCC CTGGATCCCA TGGAGCACGT TGGCATGGAG
    CCTCTGCAGT TCGATTATTC TGGCACCCAG GTACCTGTGG ACTCAGCCGC AGCCACCGTG
    GGGCTCTTCG ATTACAATTC CCAACAGCAG TTGTTCCAAA GACCGAATGC ACTTGCTGTT
    CAGCAGCTAA CAGCAGCCCA GCAGCAGCAG TATGCATTGG CAGCTGCCCA TCAGCCTCAC
    ATAGGTATGT TTTCAGCAGG TTTAGCTCCT GCTGCCTTTG TCCCCAATCC GTACATCATC
    AGCGCTGCTC CACCAGGAAC GGATCCGTAC GCAGCCGGGC TCGCAGCAGC TGCCACATTA
    GGCCCTGCAG TTGTCCCTCA CCAGTACTAC GGAGTTACAC CCTGGGGAGT TTATCCTGCC
    AGCCTCTTCC AGCAGCAAGC TGCAGCAGCT GCCGCTGCCA CTAACTCAGC AAATCAGCAG
    ACCACTCAGC AAACCCAGCA GGGCCAGCAA CAGGTTCTAC GTGGCGGAGC CAGTCAGCGT
    CCTTTGACCC CAAACCAGAA CCAGCAGGGA CAGCAGACTG ATCCGCTGGT GGCTGCCGCC
    GCCGTCAACT CTGCCCTTGC CTTTGGACAA GGGCTGGCAG CAGGGATGCC AGGGTACCCT
    GTGCTGGCTC CTGCTGCTTA CTATGACCAA ACTGGTGCTC TTGTGGTGAA TGCGGGGGCC
    AGGAATGGTC TGGGGGCACC CGTGCGTCTG GTGGCCCCTG CACCAGTTAT CATCAGCTCC
    TCTGCAGCAC AGGCAGCAGT TGCAGCAGCG GCAGCTTCAG CCAACGGTGC TGCGGGGGGC
    CTGGCAGGAA CCACCAACGG CCCCTTCCGC CCCCTGGGAA CGCAGCAGCC CCAGCCTCAG
    CCGCAGCAGC AGCCCACCAA CAACTTGGCC TCCAGCTCGT TTTATGGCAA CAACTCACTC
    AGCAGCAATT CCCAGAGCAG CTCCCTCTTC TCTCAGGGCT CTGCCCAGCC TGCCAACACC
    TCCCTGGGCT TTGGGAGCAG CAGCTCCCTT GGTGCCACTC TGGGGTCTGC ACTGGGAGGC
    TTTGGGACAG CAGTTGCTAA CTCCAACACT GGCAGCGGCT CTCGCCGAGA TTCCCTGACA
    GGGAGCAGTG ACCTGTACAA GAGGACATCC AGCAGTTTGA CACCTATAGG ACACAGTTTT
    TACAATGGCC TTGGGTTTTC CTCCTCTCCT GGACCTGTGG GTATGCCTCT GCCCAGCCAA
    GGACCTGGCC ACTCTCAGAC TCCACCACCT TCCTTATCTT CACATGGATC TTCTTCCAGT
    CTAAACCTGG GAGGGCTCAC AAATGGGAGC GGCCGTTACA TCTCTGCTGC TCCTGGAGCT
    GAAGCCAAGT ACCGCAGTGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACACTG
    TTCCCTTCGT CTCGCCTGCG CTATGGCATG TCTGATGTTA TGCCCTCCGG CCGAAGCAGG
    CTGCTCGAAG ACTTCCGCAA TAACCGGTAC CCCAATTTAC AGCTGAGGGA GATTGCTGGG
    CACATCATGG AGTTCTCCCA GGACCAGCAT GGATCCAGAT TTATTCAGCT GAAACTGGAG
    CGTGCTACCC CAGCAGAACG TCAGCTTGTG TTCAACGAGA TCCTCCAGGC GGCTTATCAG
    TTGATGGTGG ATGTATTTGG AAATTATGTC ATCCAGAAGT TCTTTGAATT TGGCAGCCTG
    GAGCAAAAGT TAGCCTTGGC AGAACGTATC CGTGGGCATG TTCTGTCCCT GGCTTTGCAG
    ATGTATGGAT GTAGGGTGAT CCAGAAGGCC CTTGAGTTTA TTCCCCCAGA CCAGCAGGTA
    ATTAACGAGA TGGTACGGGA GTTGGATGGC CATGTCCTGA AGTGTGTAAA AGACCAGAAC
    GGTAATCATG TGGTGCAGAA GTGTATTGAG TGTGTGCAGC CTCAGTCCCT GCAGTTTATC
    ATTGATGCAT TTAAGGGACA GGTTTTTGCG TTGTCTACAC ATCCATATGG CTGTCGTGTG
    ATTCAGAGGA TCCTCGAGCA CTGTCTTCCT GAGCAGACTC TTCCCATCTT GGAGGAGCTT
    CACCAGCACA CTGAGCAGCT TGTGCAGGAT CAGTATGGGA ACTATGTTAT CCAGCATGTA
    CTAGAGCACG GTCGGCCTGA GGACAAGAGC AAGATTGTAG CAGAAATTAG AGGCAACGTG
    CTCGTCTTGA GTCAACATAA ATTTGCTAGC AACGTTGTGG AGAAGTGTGT GACCCACGCC
    TCCCGTACGG AGCGTGCCAT GTTGATCGAT GAGGTGTGCA CTATGAACGA TGGCCCTCAC
    AGTGCCTTAT ACACCATGAT GAAGGATCAG TATGCCAACT ACGTGGTACA GAAGATGATC
    GATGTGGCAG AACCAGCTCA GCGGAAGATT GTCATGCACA AGATCCGGCC CCACATCGCC
    ACCCTGCGCA AGTACACCTA CGGCAAGCAC ATCTTGGCCA AGCTGGAGAA GTACTACATG
    AAGAACGGCG TTGACCTCGG GCCCATCTGT GGACCTCCAA ATGGCATCAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024998746.1
    CDS60..3353
    Translation

    Target ORF information:

    RefSeq Version XM_025142978.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGGGGCCAAG GGATGCAGAG AATGATGAGA ATGACAAAGG GGATAAGAAA
    AATAAGGGCA CATTTGATGG CGATAAATTA GGAGATCTGA AGGAGGAGGG GGATGTGATG
    GATAAAACAA ATGGTTTGCC TGTGCAGAAT GGAATCGACA CAGATGTCAA AGACTTCAGC
    CGTACACCTG GTAATTGCCA GAACTCTGCT AGTGAAGTTG ATCTTCTGGG TCCAAACCAG
    AATGGATCTG AGGGCTTAGC CCAGCTGGCG AGTACTAATG GTGCCAAGCC CGTGGAGGAT
    TTTTCCAACA TAGAGTCACA GAGTGTTCCC CTGGATCCCA TGGAGCACGT TGGCATGGAG
    CCTCTGCAGT TCGATTATTC TGGCACCCAG GTACCTGTGG ACTCAGCCGC AGCCACCGTG
    GGGCTCTTCG ATTACAATTC CCAACAGCAG TTGTTCCAAA GACCGAATGC ACTTGCTGTT
    CAGCAGCTAA CAGCAGCCCA GCAGCAGCAG TATGCATTGG CAGCTGCCCA TCAGCCTCAC
    ATAGGTATGT TTTCAGCAGG TTTAGCTCCT GCTGCCTTTG TCCCCAATCC GTACATCATC
    AGCGCTGCTC CACCAGGAAC GGATCCGTAC GCAGCCGGGC TCGCAGCAGC TGCCACATTA
    GGCCCTGCAG TTGTCCCTCA CCAGTACTAC GGAGTTACAC CCTGGGGAGT TTATCCTGCC
    AGCCTCTTCC AGCAGCAAGC TGCAGCAGCT GCCGCTGCCA CTAACTCAGC AAATCAGCAG
    ACCACTCAGC AAACCCAGCA GGGCCAGCAA CAGGTTCTAC GTGGCGGAGC CAGTCAGCGT
    CCTTTGACCC CAAACCAGAA CCAGCAGGGA CAGCAGACTG ATCCGCTGGT GGCTGCCGCC
    GCCGTCAACT CTGCCCTTGC CTTTGGACAA GGGCTGGCAG CAGGGATGCC AGGGTACCCT
    GTGCTGGCTC CTGCTGCTTA CTATGACCAA ACTGGTGCTC TTGTGGTGAA TGCGGGGGCC
    AGGAATGGTC TGGGGGCACC CGTGCGTCTG GTGGCCCCTG CACCAGTTAT CATCAGCTCC
    TCTGCAGCAC AGGCAGCAGT TGCAGCAGCG GCAGCTTCAG CCAACGGTGC TGCGGGGGGC
    CTGGCAGGAA CCACCAACGG CCCCTTCCGC CCCCTGGGAA CGCAGCAGCC CCAGCCTCAG
    CCGCAGCAGC AGCCCACCAA CAACTTGGCC TCCAGCTCGT TTTATGGCAA CAACTCACTC
    AGCAGCAATT CCCAGAGCAG CTCCCTCTTC TCTCAGGGCT CTGCCCAGCC TGCCAACACC
    TCCCTGGGCT TTGGGAGCAG CAGCTCCCTT GGTGCCACTC TGGGGTCTGC ACTGGGAGGC
    TTTGGGACAG CAGTTGCTAA CTCCAACACT GGCAGCGGCT CTCGCCGAGA TTCCCTGACA
    GGGAGCAGTG ACCTGTACAA GAGGACATCC AGCAGTTTGA CACCTATAGG ACACAGTTTT
    TACAATGGCC TTGGGTTTTC CTCCTCTCCT GGACCTGTGG GTATGCCTCT GCCCAGCCAA
    GGACCTGGCC ACTCTCAGAC TCCACCACCT TCCTTATCTT CACATGGATC TTCTTCCAGT
    CTAAACCTGG GAGGGCTCAC AAATGGGAGC GGCCGTTACA TCTCTGCTGC TCCTGGAGCT
    GAAGCCAAGT ACCGCAGTGC AAGCAGTGCC TCCAGCCTCT TCAGCCCCAG CAGCACACTG
    TTCCCTTCGT CTCGCCTGCG CTATGGCATG TCTGATGTTA TGCCCTCCGG CCGAAGCAGG
    CTGCTCGAAG ACTTCCGCAA TAACCGGTAC CCCAATTTAC AGCTGAGGGA GATTGCTGGG
    CACATCATGG AGTTCTCCCA GGACCAGCAT GGATCCAGAT TTATTCAGCT GAAACTGGAG
    CGTGCTACCC CAGCAGAACG TCAGCTTGTG TTCAACGAGA TCCTCCAGGC GGCTTATCAG
    TTGATGGTGG ATGTATTTGG AAATTATGTC ATCCAGAAGT TCTTTGAATT TGGCAGCCTG
    GAGCAAAAGT TAGCCTTGGC AGAACGTATC CGTGGGCATG TTCTGTCCCT GGCTTTGCAG
    ATGTATGGAT GTAGGGTGAT CCAGAAGGCC CTTGAGTTTA TTCCCCCAGA CCAGCAGGTA
    ATTAACGAGA TGGTACGGGA GTTGGATGGC CATGTCCTGA AGTGTGTAAA AGACCAGAAC
    GGTAATCATG TGGTGCAGAA GTGTATTGAG TGTGTGCAGC CTCAGTCCCT GCAGTTTATC
    ATTGATGCAT TTAAGGGACA GGTTTTTGCG TTGTCTACAC ATCCATATGG CTGTCGTGTG
    ATTCAGAGGA TCCTCGAGCA CTGTCTTCCT GAGCAGACTC TTCCCATCTT GGAGGAGCTT
    CACCAGCACA CTGAGCAGCT TGTGCAGGAT CAGTATGGGA ACTATGTTAT CCAGCATGTA
    CTAGAGCACG GTCGGCCTGA GGACAAGAGC AAGATTGTAG CAGAAATTAG AGGCAACGTG
    CTCGTCTTGA GTCAACATAA ATTTGCTAGC AACGTTGTGG AGAAGTGTGT GACCCACGCC
    TCCCGTACGG AGCGTGCCAT GTTGATCGAT GAGGTGTGCA CTATGAACGA TGGCCCTCAC
    AGTGCCTTAT ACACCATGAT GAAGGATCAG TATGCCAACT ACGTGGTACA GAAGATGATC
    GATGTGGCAG AACCAGCTCA GCGGAAGATT GTCATGCACA AGATCCGGCC CCACATCGCC
    ACCCTGCGCA AGTACACCTA CGGCAAGCAC ATCTTGGCCA AGCTGGAGAA GTACTACATG
    AAGAACGGCG TTGACCTCGG GCCCATCTGT GGACCTCCAA ATGGCATCAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147398
    Clone ID Related Accession (Same CDS sequence) XM_025142979.1
    Accession Version XM_025142979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGGGGCCAAG GGATGCAGAG AATGATGAGA ATGACAAAGG GGATAAGAAA
    AATAAGGGCA CATTTGATGG CGATAAATTA GGAGATCTGA AGGAGGAGGG GGATGTGATG
    GATAAAACAA ATGGTTTGCC TGTGCAGAAT GGAATCGACA CAGATGTCAA AGACTTCAGC
    CGTACACCTG GTAATTGCCA GAACTCTGCT AGTGAAGTTG ATCTTCTGGG TCCAAACCAG
    AATGGATCTG AGGGCTTAGC CCAGCTGGCG AGTACTAATG GTGCCAAGCC CGTGGAGGAT
    TTTTCCAACA TAGAGTCACA GAGTGTTCCC CTGGATCCCA TGGAGCACGT TGGCATGGAG
    CCTCTGCAGT TCGATTATTC TGGCACCCAG GTACCTGTGG ACTCAGCCGC AGCCACCGTG
    GGGCTCTTCG ATTACAATTC CCAACAGCAG TTGTTCCAAA GACCGAATGC ACTTGCTGTT
    CAGCAGCTAA CAGCAGCCCA GCAGCAGCAG TATGCATTGG CAGCTGCCCA TCAGCCTCAC
    ATAGGTTTAG CTCCTGCTGC CTTTGTCCCC AATCCGTACA TCATCAGCGC TGCTCCACCA
    GGAACGGATC CGTACGCAGC CGGGCTCGCA GCAGCTGCCA CATTAGGCCC TGCAGTTGTC
    CCTCACCAGT ACTACGGAGT TACACCCTGG GGAGTTTATC CTGCCAGCCT CTTCCAGCAG
    CAAGCTGCAG CAGCTGCCGC TGCCACTAAC TCAGCAAATC AGCAGACCAC TCAGCAAACC
    CAGCAGGGCC AGCAACAGGT TCTACGTGGC GGAGCCAGTC AGCGTCCTTT GACCCCAAAC
    CAGAACCAGC AGGGACAGCA GACTGATCCG CTGGTGGCTG CCGCCGCCGT CAACTCTGCC
    CTTGCCTTTG GACAAGGGCT GGCAGCAGGG ATGCCAGGGT ACCCTGTGCT GGCTCCTGCT
    GCTTACTATG ACCAAACTGG TGCTCTTGTG GTGAATGCGG GGGCCAGGAA TGGTCTGGGG
    GCACCCGTGC GTCTGGTGGC CCCTGCACCA GTTATCATCA GCTCCTCTGC AGCACAGGCA
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024998747.1
    CDS60..3338
    Translation

    Target ORF information:

    RefSeq Version XM_025142979.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGGGGCCAAG GGATGCAGAG AATGATGAGA ATGACAAAGG GGATAAGAAA
    AATAAGGGCA CATTTGATGG CGATAAATTA GGAGATCTGA AGGAGGAGGG GGATGTGATG
    GATAAAACAA ATGGTTTGCC TGTGCAGAAT GGAATCGACA CAGATGTCAA AGACTTCAGC
    CGTACACCTG GTAATTGCCA GAACTCTGCT AGTGAAGTTG ATCTTCTGGG TCCAAACCAG
    AATGGATCTG AGGGCTTAGC CCAGCTGGCG AGTACTAATG GTGCCAAGCC CGTGGAGGAT
    TTTTCCAACA TAGAGTCACA GAGTGTTCCC CTGGATCCCA TGGAGCACGT TGGCATGGAG
    CCTCTGCAGT TCGATTATTC TGGCACCCAG GTACCTGTGG ACTCAGCCGC AGCCACCGTG
    GGGCTCTTCG ATTACAATTC CCAACAGCAG TTGTTCCAAA GACCGAATGC ACTTGCTGTT
    CAGCAGCTAA CAGCAGCCCA GCAGCAGCAG TATGCATTGG CAGCTGCCCA TCAGCCTCAC
    ATAGGTTTAG CTCCTGCTGC CTTTGTCCCC AATCCGTACA TCATCAGCGC TGCTCCACCA
    GGAACGGATC CGTACGCAGC CGGGCTCGCA GCAGCTGCCA CATTAGGCCC TGCAGTTGTC
    CCTCACCAGT ACTACGGAGT TACACCCTGG GGAGTTTATC CTGCCAGCCT CTTCCAGCAG
    CAAGCTGCAG CAGCTGCCGC TGCCACTAAC TCAGCAAATC AGCAGACCAC TCAGCAAACC
    CAGCAGGGCC AGCAACAGGT TCTACGTGGC GGAGCCAGTC AGCGTCCTTT GACCCCAAAC
    CAGAACCAGC AGGGACAGCA GACTGATCCG CTGGTGGCTG CCGCCGCCGT CAACTCTGCC
    CTTGCCTTTG GACAAGGGCT GGCAGCAGGG ATGCCAGGGT ACCCTGTGCT GGCTCCTGCT
    GCTTACTATG ACCAAACTGG TGCTCTTGTG GTGAATGCGG GGGCCAGGAA TGGTCTGGGG
    GCACCCGTGC GTCTGGTGGC CCCTGCACCA GTTATCATCA GCTCCTCTGC AGCACAGGCA
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45495
    Clone ID Related Accession (Same CDS sequence) XM_015297531.2 , XM_015297531.1
    Accession Version XM_015297531.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153017.1
    CDS60..3641
    Translation

    Target ORF information:

    RefSeq Version XM_015297531.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45495
    Clone ID Related Accession (Same CDS sequence) XM_015297531.2 , XM_015297531.1
    Accession Version XM_015297531.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153017.1
    CDS121..3702
    Translation

    Target ORF information:

    RefSeq Version XM_015297531.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45496
    Clone ID Related Accession (Same CDS sequence) XM_015297532.1 , XM_015297532.2
    Accession Version XM_015297532.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153018.1
    CDS121..3699
    Translation

    Target ORF information:

    RefSeq Version XM_015297532.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45496
    Clone ID Related Accession (Same CDS sequence) XM_015297532.1 , XM_015297532.2
    Accession Version XM_015297532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153018.1
    CDS60..3638
    Translation

    Target ORF information:

    RefSeq Version XM_015297532.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45497
    Clone ID Related Accession (Same CDS sequence) XM_015297533.1 , XM_015297533.2
    Accession Version XM_015297533.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCTGTTGCC GGGTAAAAAG TTTTGGGAAT CTGATGATTC CAGCAAAGAT
    GGACCAAAAG GGATATTTCT GGGAGATCAG TGGAGAGACA GTGCTTGGGG AACATCAGAT
    CACTCTGTTT CCCAACCAAT TATGGTTCAG AGAAGACCTG GTCAGGGCTT TCATGTGAAT
    AGTGAAGTCA ACTCAGTGCT TTCACCACGG TCAGAGAGTG GAGGACTTGG AGTTAGCATG
    GTGGAATATG TGTTGAGCTC ATCTCCCGGA GATTCCTGCC TAAGGAAAGG AGGATTTGGG
    CCAAGGGATG CAGAGAATGA TGAGAATGAC AAAGGGGATA AGAAAAATAA GGGCACATTT
    GATGGCGATA AATTAGGAGA TCTGAAGGAG GAGGGGGATG TGATGGATAA AACAAATGGT
    TTGCCTGTGC AGAATGGAAT CGACACAGAT GTCAAAGACT TCAGCCGTAC ACCTGGTAAT
    TGCCAGAACT CTGCTAGTGA AGTTGATCTT CTGGGTCCAA ACCAGAATGG ATCTGAGGGC
    TTAGCCCAGC TGGCGAGTAC TAATGGTGCC AAGCCCGTGG AGGATTTTTC CAACATAGAG
    TCACAGAGTG TTCCCCTGGA TCCCATGGAG CACGTTGGCA TGGAGCCTCT GCAGTTCGAT
    TATTCTGGCA CCCAGGTACC TGTGGACTCA GCCGCAGCCA CCGTGGGGCT CTTCGATTAC
    AATTCCCAAC AGCAGTTGTT CCAAAGACCG AATGCACTTG CTGTTCAGCA GCTAACAGCA
    GCCCAGCAGC AGCAGTATGC ATTGGCAGCT GCCCATCAGC CTCACATAGG TATGTTTTCA
    GCAGGTTTAG CTCCTGCTGC CTTTGTCCCC AATCCGTACA TCATCAGCGC TGCTCCACCA
    GGAACGGATC CGTACGCAGC CGGGCTCGCA GCAGCTGCCA CATTAGGCCC TGCAGTTGTC
    CCTCACCAGT ACTACGGAGT TACACCCTGG GGAGTTTATC CTGCCAGCCT CTTCCAGCAG
    CAAGCTGCAG CAGCTGCCGC TGCCACTAAC TCAGCAAATC AGCAGACCAC TCAGCAAACC
    CAGCAGGGCC AGCAACAGGT TCTACGTGGC GGAGCCAGTC AGCGTCCTTT GACCCCAAAC
    CAGAACCAGC AGGGACAGCA GACTGATCCG CTGGTGGCTG CCGCCGCCGT CAACTCTGCC
    CTTGCCTTTG GACAAGGGCT GGCAGCAGGG ATGCCAGGGT ACCCTGTGCT GGCTCCTGCT
    GCTTACTATG ACCAAACTGG TGCTCTTGTG GTGAATGCGG GGGCCAGGAA TGGTCTGGGG
    GCACCCGTGC GTCTGGTGGC CCCTGCACCA GTTATCATCA GCTCCTCTGC AGCACAGGCA
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153019.1
    CDS121..3699
    Translation

    Target ORF information:

    RefSeq Version XM_015297533.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297533.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCTGTTGCC GGGTAAAAAG TTTTGGGAAT CTGATGATTC CAGCAAAGAT
    GGACCAAAAG GGATATTTCT GGGAGATCAG TGGAGAGACA GTGCTTGGGG AACATCAGAT
    CACTCTGTTT CCCAACCAAT TATGGTTCAG AGAAGACCTG GTCAGGGCTT TCATGTGAAT
    AGTGAAGTCA ACTCAGTGCT TTCACCACGG TCAGAGAGTG GAGGACTTGG AGTTAGCATG
    GTGGAATATG TGTTGAGCTC ATCTCCCGGA GATTCCTGCC TAAGGAAAGG AGGATTTGGG
    CCAAGGGATG CAGAGAATGA TGAGAATGAC AAAGGGGATA AGAAAAATAA GGGCACATTT
    GATGGCGATA AATTAGGAGA TCTGAAGGAG GAGGGGGATG TGATGGATAA AACAAATGGT
    TTGCCTGTGC AGAATGGAAT CGACACAGAT GTCAAAGACT TCAGCCGTAC ACCTGGTAAT
    TGCCAGAACT CTGCTAGTGA AGTTGATCTT CTGGGTCCAA ACCAGAATGG ATCTGAGGGC
    TTAGCCCAGC TGGCGAGTAC TAATGGTGCC AAGCCCGTGG AGGATTTTTC CAACATAGAG
    TCACAGAGTG TTCCCCTGGA TCCCATGGAG CACGTTGGCA TGGAGCCTCT GCAGTTCGAT
    TATTCTGGCA CCCAGGTACC TGTGGACTCA GCCGCAGCCA CCGTGGGGCT CTTCGATTAC
    AATTCCCAAC AGCAGTTGTT CCAAAGACCG AATGCACTTG CTGTTCAGCA GCTAACAGCA
    GCCCAGCAGC AGCAGTATGC ATTGGCAGCT GCCCATCAGC CTCACATAGG TATGTTTTCA
    GCAGGTTTAG CTCCTGCTGC CTTTGTCCCC AATCCGTACA TCATCAGCGC TGCTCCACCA
    GGAACGGATC CGTACGCAGC CGGGCTCGCA GCAGCTGCCA CATTAGGCCC TGCAGTTGTC
    CCTCACCAGT ACTACGGAGT TACACCCTGG GGAGTTTATC CTGCCAGCCT CTTCCAGCAG
    CAAGCTGCAG CAGCTGCCGC TGCCACTAAC TCAGCAAATC AGCAGACCAC TCAGCAAACC
    CAGCAGGGCC AGCAACAGGT TCTACGTGGC GGAGCCAGTC AGCGTCCTTT GACCCCAAAC
    CAGAACCAGC AGGGACAGCA GACTGATCCG CTGGTGGCTG CCGCCGCCGT CAACTCTGCC
    CTTGCCTTTG GACAAGGGCT GGCAGCAGGG ATGCCAGGGT ACCCTGTGCT GGCTCCTGCT
    GCTTACTATG ACCAAACTGG TGCTCTTGTG GTGAATGCGG GGGCCAGGAA TGGTCTGGGG
    GCACCCGTGC GTCTGGTGGC CCCTGCACCA GTTATCATCA GCTCCTCTGC AGCACAGGCA
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45497
    Clone ID Related Accession (Same CDS sequence) XM_015297533.1 , XM_015297533.2
    Accession Version XM_015297533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCTGTTGCC GGGTAAAAAG TTTTGGGAAT CTGATGATTC CAGCAAAGAT
    GGACCAAAAG GGATATTTCT GGGAGATCAG TGGAGAGACA GTGCTTGGGG AACATCAGAT
    CACTCTGTTT CCCAACCAAT TATGGTTCAG AGAAGACCTG GTCAGGGCTT TCATGTGAAT
    AGTGAAGTCA ACTCAGTGCT TTCACCACGG TCAGAGAGTG GAGGACTTGG AGTTAGCATG
    GTGGAATATG TGTTGAGCTC ATCTCCCGGA GATTCCTGCC TAAGGAAAGG AGGATTTGGG
    CCAAGGGATG CAGAGAATGA TGAGAATGAC AAAGGGGATA AGAAAAATAA GGGCACATTT
    GATGGCGATA AATTAGGAGA TCTGAAGGAG GAGGGGGATG TGATGGATAA AACAAATGGT
    TTGCCTGTGC AGAATGGAAT CGACACAGAT GTCAAAGACT TCAGCCGTAC ACCTGGTAAT
    TGCCAGAACT CTGCTAGTGA AGTTGATCTT CTGGGTCCAA ACCAGAATGG ATCTGAGGGC
    TTAGCCCAGC TGGCGAGTAC TAATGGTGCC AAGCCCGTGG AGGATTTTTC CAACATAGAG
    TCACAGAGTG TTCCCCTGGA TCCCATGGAG CACGTTGGCA TGGAGCCTCT GCAGTTCGAT
    TATTCTGGCA CCCAGGTACC TGTGGACTCA GCCGCAGCCA CCGTGGGGCT CTTCGATTAC
    AATTCCCAAC AGCAGTTGTT CCAAAGACCG AATGCACTTG CTGTTCAGCA GCTAACAGCA
    GCCCAGCAGC AGCAGTATGC ATTGGCAGCT GCCCATCAGC CTCACATAGG TATGTTTTCA
    GCAGGTTTAG CTCCTGCTGC CTTTGTCCCC AATCCGTACA TCATCAGCGC TGCTCCACCA
    GGAACGGATC CGTACGCAGC CGGGCTCGCA GCAGCTGCCA CATTAGGCCC TGCAGTTGTC
    CCTCACCAGT ACTACGGAGT TACACCCTGG GGAGTTTATC CTGCCAGCCT CTTCCAGCAG
    CAAGCTGCAG CAGCTGCCGC TGCCACTAAC TCAGCAAATC AGCAGACCAC TCAGCAAACC
    CAGCAGGGCC AGCAACAGGT TCTACGTGGC GGAGCCAGTC AGCGTCCTTT GACCCCAAAC
    CAGAACCAGC AGGGACAGCA GACTGATCCG CTGGTGGCTG CCGCCGCCGT CAACTCTGCC
    CTTGCCTTTG GACAAGGGCT GGCAGCAGGG ATGCCAGGGT ACCCTGTGCT GGCTCCTGCT
    GCTTACTATG ACCAAACTGG TGCTCTTGTG GTGAATGCGG GGGCCAGGAA TGGTCTGGGG
    GCACCCGTGC GTCTGGTGGC CCCTGCACCA GTTATCATCA GCTCCTCTGC AGCACAGGCA
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153019.1
    CDS60..3638
    Translation

    Target ORF information:

    RefSeq Version XM_015297533.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCTGTTGCC GGGTAAAAAG TTTTGGGAAT CTGATGATTC CAGCAAAGAT
    GGACCAAAAG GGATATTTCT GGGAGATCAG TGGAGAGACA GTGCTTGGGG AACATCAGAT
    CACTCTGTTT CCCAACCAAT TATGGTTCAG AGAAGACCTG GTCAGGGCTT TCATGTGAAT
    AGTGAAGTCA ACTCAGTGCT TTCACCACGG TCAGAGAGTG GAGGACTTGG AGTTAGCATG
    GTGGAATATG TGTTGAGCTC ATCTCCCGGA GATTCCTGCC TAAGGAAAGG AGGATTTGGG
    CCAAGGGATG CAGAGAATGA TGAGAATGAC AAAGGGGATA AGAAAAATAA GGGCACATTT
    GATGGCGATA AATTAGGAGA TCTGAAGGAG GAGGGGGATG TGATGGATAA AACAAATGGT
    TTGCCTGTGC AGAATGGAAT CGACACAGAT GTCAAAGACT TCAGCCGTAC ACCTGGTAAT
    TGCCAGAACT CTGCTAGTGA AGTTGATCTT CTGGGTCCAA ACCAGAATGG ATCTGAGGGC
    TTAGCCCAGC TGGCGAGTAC TAATGGTGCC AAGCCCGTGG AGGATTTTTC CAACATAGAG
    TCACAGAGTG TTCCCCTGGA TCCCATGGAG CACGTTGGCA TGGAGCCTCT GCAGTTCGAT
    TATTCTGGCA CCCAGGTACC TGTGGACTCA GCCGCAGCCA CCGTGGGGCT CTTCGATTAC
    AATTCCCAAC AGCAGTTGTT CCAAAGACCG AATGCACTTG CTGTTCAGCA GCTAACAGCA
    GCCCAGCAGC AGCAGTATGC ATTGGCAGCT GCCCATCAGC CTCACATAGG TATGTTTTCA
    GCAGGTTTAG CTCCTGCTGC CTTTGTCCCC AATCCGTACA TCATCAGCGC TGCTCCACCA
    GGAACGGATC CGTACGCAGC CGGGCTCGCA GCAGCTGCCA CATTAGGCCC TGCAGTTGTC
    CCTCACCAGT ACTACGGAGT TACACCCTGG GGAGTTTATC CTGCCAGCCT CTTCCAGCAG
    CAAGCTGCAG CAGCTGCCGC TGCCACTAAC TCAGCAAATC AGCAGACCAC TCAGCAAACC
    CAGCAGGGCC AGCAACAGGT TCTACGTGGC GGAGCCAGTC AGCGTCCTTT GACCCCAAAC
    CAGAACCAGC AGGGACAGCA GACTGATCCG CTGGTGGCTG CCGCCGCCGT CAACTCTGCC
    CTTGCCTTTG GACAAGGGCT GGCAGCAGGG ATGCCAGGGT ACCCTGTGCT GGCTCCTGCT
    GCTTACTATG ACCAAACTGG TGCTCTTGTG GTGAATGCGG GGGCCAGGAA TGGTCTGGGG
    GCACCCGTGC GTCTGGTGGC CCCTGCACCA GTTATCATCA GCTCCTCTGC AGCACAGGCA
    GCAGTTGCAG CAGCGGCAGC TTCAGCCAAC GGTGCTGCGG GGGGCCTGGC AGGAACCACC
    AACGGCCCCT TCCGCCCCCT GGGAACGCAG CAGCCCCAGC CTCAGCCGCA GCAGCAGCCC
    ACCAACAACT TGGCCTCCAG CTCGTTTTAT GGCAACAACT CACTCAGCAG CAATTCCCAG
    AGCAGCTCCC TCTTCTCTCA GGGCTCTGCC CAGCCTGCCA ACACCTCCCT GGGCTTTGGG
    AGCAGCAGCT CCCTTGGTGC CACTCTGGGG TCTGCACTGG GAGGCTTTGG GACAGCAGTT
    GCTAACTCCA ACACTGGCAG CGGCTCTCGC CGAGATTCCC TGACAGGGAG CAGTGACCTG
    TACAAGAGGA CATCCAGCAG TTTGACACCT ATAGGACACA GTTTTTACAA TGGCCTTGGG
    TTTTCCTCCT CTCCTGGACC TGTGGGTATG CCTCTGCCCA GCCAAGGACC TGGCCACTCT
    CAGACTCCAC CACCTTCCTT ATCTTCACAT GGATCTTCTT CCAGTCTAAA CCTGGGAGGG
    CTCACAAATG GGAGCGGCCG TTACATCTCT GCTGCTCCTG GAGCTGAAGC CAAGTACCGC
    AGTGCAAGCA GTGCCTCCAG CCTCTTCAGC CCCAGCAGCA CACTGTTCCC TTCGTCTCGC
    CTGCGCTATG GCATGTCTGA TGTTATGCCC TCCGGCCGAA GCAGGCTGCT CGAAGACTTC
    CGCAATAACC GGTACCCCAA TTTACAGCTG AGGGAGATTG CTGGGCACAT CATGGAGTTC
    TCCCAGGACC AGCATGGATC CAGATTTATT CAGCTGAAAC TGGAGCGTGC TACCCCAGCA
    GAACGTCAGC TTGTGTTCAA CGAGATCCTC CAGGCGGCTT ATCAGTTGAT GGTGGATGTA
    TTTGGAAATT ATGTCATCCA GAAGTTCTTT GAATTTGGCA GCCTGGAGCA AAAGTTAGCC
    TTGGCAGAAC GTATCCGTGG GCATGTTCTG TCCCTGGCTT TGCAGATGTA TGGATGTAGG
    GTGATCCAGA AGGCCCTTGA GTTTATTCCC CCAGACCAGC AGGTAATTAA CGAGATGGTA
    CGGGAGTTGG ATGGCCATGT CCTGAAGTGT GTAAAAGACC AGAACGGTAA TCATGTGGTG
    CAGAAGTGTA TTGAGTGTGT GCAGCCTCAG TCCCTGCAGT TTATCATTGA TGCATTTAAG
    GGACAGGTTT TTGCGTTGTC TACACATCCA TATGGCTGTC GTGTGATTCA GAGGATCCTC
    GAGCACTGTC TTCCTGAGCA GACTCTTCCC ATCTTGGAGG AGCTTCACCA GCACACTGAG
    CAGCTTGTGC AGGATCAGTA TGGGAACTAT GTTATCCAGC ATGTACTAGA GCACGGTCGG
    CCTGAGGACA AGAGCAAGAT TGTAGCAGAA ATTAGAGGCA ACGTGCTCGT CTTGAGTCAA
    CATAAATTTG CTAGCAACGT TGTGGAGAAG TGTGTGACCC ACGCCTCCCG TACGGAGCGT
    GCCATGTTGA TCGATGAGGT GTGCACTATG AACGATGGCC CTCACAGTGC CTTATACACC
    ATGATGAAGG ATCAGTATGC CAACTACGTG GTACAGAAGA TGATCGATGT GGCAGAACCA
    GCTCAGCGGA AGATTGTCAT GCACAAGATC CGGCCCCACA TCGCCACCCT GCGCAAGTAC
    ACCTACGGCA AGCACATCTT GGCCAAGCTG GAGAAGTACT ACATGAAGAA CGGCGTTGAC
    CTCGGGCCCA TCTGTGGACC TCCAAATGGC ATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45498
    Clone ID Related Accession (Same CDS sequence) XM_015297534.1 , XM_015297534.2
    Accession Version XM_015297534.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGAACGA GATGGTACGG
    GAGTTGGATG GCCATGTCCT GAAGTGTGTA AAAGACCAGA ACGGTAATCA TGTGGTGCAG
    AAGTGTATTG AGTGTGTGCA GCCTCAGTCC CTGCAGTTTA TCATTGATGC ATTTAAGGGA
    CAGGTTTTTG CGTTGTCTAC ACATCCATAT GGCTGTCGTG TGATTCAGAG GATCCTCGAG
    CACTGTCTTC CTGAGCAGAC TCTTCCCATC TTGGAGGAGC TTCACCAGCA CACTGAGCAG
    CTTGTGCAGG ATCAGTATGG GAACTATGTT ATCCAGCATG TACTAGAGCA CGGTCGGCCT
    GAGGACAAGA GCAAGATTGT AGCAGAAATT AGAGGCAACG TGCTCGTCTT GAGTCAACAT
    AAATTTGCTA GCAACGTTGT GGAGAAGTGT GTGACCCACG CCTCCCGTAC GGAGCGTGCC
    ATGTTGATCG ATGAGGTGTG CACTATGAAC GATGGCCCTC ACAGTGCCTT ATACACCATG
    ATGAAGGATC AGTATGCCAA CTACGTGGTA CAGAAGATGA TCGATGTGGC AGAACCAGCT
    CAGCGGAAGA TTGTCATGCA CAAGATCCGG CCCCACATCG CCACCCTGCG CAAGTACACC
    TACGGCAAGC ACATCTTGGC CAAGCTGGAG AAGTACTACA TGAAGAACGG CGTTGACCTC
    GGGCCCATCT GTGGACCTCC AAATGGCATC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153020.1
    CDS121..3696
    Translation

    Target ORF information:

    RefSeq Version XM_015297534.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGAACGA GATGGTACGG
    GAGTTGGATG GCCATGTCCT GAAGTGTGTA AAAGACCAGA ACGGTAATCA TGTGGTGCAG
    AAGTGTATTG AGTGTGTGCA GCCTCAGTCC CTGCAGTTTA TCATTGATGC ATTTAAGGGA
    CAGGTTTTTG CGTTGTCTAC ACATCCATAT GGCTGTCGTG TGATTCAGAG GATCCTCGAG
    CACTGTCTTC CTGAGCAGAC TCTTCCCATC TTGGAGGAGC TTCACCAGCA CACTGAGCAG
    CTTGTGCAGG ATCAGTATGG GAACTATGTT ATCCAGCATG TACTAGAGCA CGGTCGGCCT
    GAGGACAAGA GCAAGATTGT AGCAGAAATT AGAGGCAACG TGCTCGTCTT GAGTCAACAT
    AAATTTGCTA GCAACGTTGT GGAGAAGTGT GTGACCCACG CCTCCCGTAC GGAGCGTGCC
    ATGTTGATCG ATGAGGTGTG CACTATGAAC GATGGCCCTC ACAGTGCCTT ATACACCATG
    ATGAAGGATC AGTATGCCAA CTACGTGGTA CAGAAGATGA TCGATGTGGC AGAACCAGCT
    CAGCGGAAGA TTGTCATGCA CAAGATCCGG CCCCACATCG CCACCCTGCG CAAGTACACC
    TACGGCAAGC ACATCTTGGC CAAGCTGGAG AAGTACTACA TGAAGAACGG CGTTGACCTC
    GGGCCCATCT GTGGACCTCC AAATGGCATC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45498
    Clone ID Related Accession (Same CDS sequence) XM_015297534.1 , XM_015297534.2
    Accession Version XM_015297534.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGAACGA GATGGTACGG
    GAGTTGGATG GCCATGTCCT GAAGTGTGTA AAAGACCAGA ACGGTAATCA TGTGGTGCAG
    AAGTGTATTG AGTGTGTGCA GCCTCAGTCC CTGCAGTTTA TCATTGATGC ATTTAAGGGA
    CAGGTTTTTG CGTTGTCTAC ACATCCATAT GGCTGTCGTG TGATTCAGAG GATCCTCGAG
    CACTGTCTTC CTGAGCAGAC TCTTCCCATC TTGGAGGAGC TTCACCAGCA CACTGAGCAG
    CTTGTGCAGG ATCAGTATGG GAACTATGTT ATCCAGCATG TACTAGAGCA CGGTCGGCCT
    GAGGACAAGA GCAAGATTGT AGCAGAAATT AGAGGCAACG TGCTCGTCTT GAGTCAACAT
    AAATTTGCTA GCAACGTTGT GGAGAAGTGT GTGACCCACG CCTCCCGTAC GGAGCGTGCC
    ATGTTGATCG ATGAGGTGTG CACTATGAAC GATGGCCCTC ACAGTGCCTT ATACACCATG
    ATGAAGGATC AGTATGCCAA CTACGTGGTA CAGAAGATGA TCGATGTGGC AGAACCAGCT
    CAGCGGAAGA TTGTCATGCA CAAGATCCGG CCCCACATCG CCACCCTGCG CAAGTACACC
    TACGGCAAGC ACATCTTGGC CAAGCTGGAG AAGTACTACA TGAAGAACGG CGTTGACCTC
    GGGCCCATCT GTGGACCTCC AAATGGCATC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153020.1
    CDS60..3635
    Translation

    Target ORF information:

    RefSeq Version XM_015297534.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297534.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGAACGA GATGGTACGG
    GAGTTGGATG GCCATGTCCT GAAGTGTGTA AAAGACCAGA ACGGTAATCA TGTGGTGCAG
    AAGTGTATTG AGTGTGTGCA GCCTCAGTCC CTGCAGTTTA TCATTGATGC ATTTAAGGGA
    CAGGTTTTTG CGTTGTCTAC ACATCCATAT GGCTGTCGTG TGATTCAGAG GATCCTCGAG
    CACTGTCTTC CTGAGCAGAC TCTTCCCATC TTGGAGGAGC TTCACCAGCA CACTGAGCAG
    CTTGTGCAGG ATCAGTATGG GAACTATGTT ATCCAGCATG TACTAGAGCA CGGTCGGCCT
    GAGGACAAGA GCAAGATTGT AGCAGAAATT AGAGGCAACG TGCTCGTCTT GAGTCAACAT
    AAATTTGCTA GCAACGTTGT GGAGAAGTGT GTGACCCACG CCTCCCGTAC GGAGCGTGCC
    ATGTTGATCG ATGAGGTGTG CACTATGAAC GATGGCCCTC ACAGTGCCTT ATACACCATG
    ATGAAGGATC AGTATGCCAA CTACGTGGTA CAGAAGATGA TCGATGTGGC AGAACCAGCT
    CAGCGGAAGA TTGTCATGCA CAAGATCCGG CCCCACATCG CCACCCTGCG CAAGTACACC
    TACGGCAAGC ACATCTTGGC CAAGCTGGAG AAGTACTACA TGAAGAACGG CGTTGACCTC
    GGGCCCATCT GTGGACCTCC AAATGGCATC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45499
    Clone ID Related Accession (Same CDS sequence) XM_015297535.1 , XM_015297535.2
    Accession Version XM_015297535.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153021.1
    CDS121..3690
    Translation

    Target ORF information:

    RefSeq Version XM_015297535.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45499
    Clone ID Related Accession (Same CDS sequence) XM_015297535.1 , XM_015297535.2
    Accession Version XM_015297535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153021.1
    CDS60..3629
    Translation

    Target ORF information:

    RefSeq Version XM_015297535.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45500
    Clone ID Related Accession (Same CDS sequence) XM_015297537.1 , XM_015297537.2
    Accession Version XM_015297537.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153023.1
    CDS121..3687
    Translation

    Target ORF information:

    RefSeq Version XM_015297537.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45500
    Clone ID Related Accession (Same CDS sequence) XM_015297537.1 , XM_015297537.2
    Accession Version XM_015297537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153023.1
    CDS60..3626
    Translation

    Target ORF information:

    RefSeq Version XM_015297537.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45501
    Clone ID Related Accession (Same CDS sequence) XM_015297538.1 , XM_015297538.2
    Accession Version XM_015297538.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG AACGAGATGG TACGGGAGTT GGATGGCCAT
    GTCCTGAAGT GTGTAAAAGA CCAGAACGGT AATCATGTGG TGCAGAAGTG TATTGAGTGT
    GTGCAGCCTC AGTCCCTGCA GTTTATCATT GATGCATTTA AGGGACAGGT TTTTGCGTTG
    TCTACACATC CATATGGCTG TCGTGTGATT CAGAGGATCC TCGAGCACTG TCTTCCTGAG
    CAGACTCTTC CCATCTTGGA GGAGCTTCAC CAGCACACTG AGCAGCTTGT GCAGGATCAG
    TATGGGAACT ATGTTATCCA GCATGTACTA GAGCACGGTC GGCCTGAGGA CAAGAGCAAG
    ATTGTAGCAG AAATTAGAGG CAACGTGCTC GTCTTGAGTC AACATAAATT TGCTAGCAAC
    GTTGTGGAGA AGTGTGTGAC CCACGCCTCC CGTACGGAGC GTGCCATGTT GATCGATGAG
    GTGTGCACTA TGAACGATGG CCCTCACAGT GCCTTATACA CCATGATGAA GGATCAGTAT
    GCCAACTACG TGGTACAGAA GATGATCGAT GTGGCAGAAC CAGCTCAGCG GAAGATTGTC
    ATGCACAAGA TCCGGCCCCA CATCGCCACC CTGCGCAAGT ACACCTACGG CAAGCACATC
    TTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGCGTTG ACCTCGGGCC CATCTGTGGA
    CCTCCAAATG GCATCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153024.1
    CDS121..3681
    Translation

    Target ORF information:

    RefSeq Version XM_015297538.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG AACGAGATGG TACGGGAGTT GGATGGCCAT
    GTCCTGAAGT GTGTAAAAGA CCAGAACGGT AATCATGTGG TGCAGAAGTG TATTGAGTGT
    GTGCAGCCTC AGTCCCTGCA GTTTATCATT GATGCATTTA AGGGACAGGT TTTTGCGTTG
    TCTACACATC CATATGGCTG TCGTGTGATT CAGAGGATCC TCGAGCACTG TCTTCCTGAG
    CAGACTCTTC CCATCTTGGA GGAGCTTCAC CAGCACACTG AGCAGCTTGT GCAGGATCAG
    TATGGGAACT ATGTTATCCA GCATGTACTA GAGCACGGTC GGCCTGAGGA CAAGAGCAAG
    ATTGTAGCAG AAATTAGAGG CAACGTGCTC GTCTTGAGTC AACATAAATT TGCTAGCAAC
    GTTGTGGAGA AGTGTGTGAC CCACGCCTCC CGTACGGAGC GTGCCATGTT GATCGATGAG
    GTGTGCACTA TGAACGATGG CCCTCACAGT GCCTTATACA CCATGATGAA GGATCAGTAT
    GCCAACTACG TGGTACAGAA GATGATCGAT GTGGCAGAAC CAGCTCAGCG GAAGATTGTC
    ATGCACAAGA TCCGGCCCCA CATCGCCACC CTGCGCAAGT ACACCTACGG CAAGCACATC
    TTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGCGTTG ACCTCGGGCC CATCTGTGGA
    CCTCCAAATG GCATCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45501
    Clone ID Related Accession (Same CDS sequence) XM_015297538.1 , XM_015297538.2
    Accession Version XM_015297538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG AACGAGATGG TACGGGAGTT GGATGGCCAT
    GTCCTGAAGT GTGTAAAAGA CCAGAACGGT AATCATGTGG TGCAGAAGTG TATTGAGTGT
    GTGCAGCCTC AGTCCCTGCA GTTTATCATT GATGCATTTA AGGGACAGGT TTTTGCGTTG
    TCTACACATC CATATGGCTG TCGTGTGATT CAGAGGATCC TCGAGCACTG TCTTCCTGAG
    CAGACTCTTC CCATCTTGGA GGAGCTTCAC CAGCACACTG AGCAGCTTGT GCAGGATCAG
    TATGGGAACT ATGTTATCCA GCATGTACTA GAGCACGGTC GGCCTGAGGA CAAGAGCAAG
    ATTGTAGCAG AAATTAGAGG CAACGTGCTC GTCTTGAGTC AACATAAATT TGCTAGCAAC
    GTTGTGGAGA AGTGTGTGAC CCACGCCTCC CGTACGGAGC GTGCCATGTT GATCGATGAG
    GTGTGCACTA TGAACGATGG CCCTCACAGT GCCTTATACA CCATGATGAA GGATCAGTAT
    GCCAACTACG TGGTACAGAA GATGATCGAT GTGGCAGAAC CAGCTCAGCG GAAGATTGTC
    ATGCACAAGA TCCGGCCCCA CATCGCCACC CTGCGCAAGT ACACCTACGG CAAGCACATC
    TTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGCGTTG ACCTCGGGCC CATCTGTGGA
    CCTCCAAATG GCATCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153024.1
    CDS60..3620
    Translation

    Target ORF information:

    RefSeq Version XM_015297538.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG AACGAGATGG TACGGGAGTT GGATGGCCAT
    GTCCTGAAGT GTGTAAAAGA CCAGAACGGT AATCATGTGG TGCAGAAGTG TATTGAGTGT
    GTGCAGCCTC AGTCCCTGCA GTTTATCATT GATGCATTTA AGGGACAGGT TTTTGCGTTG
    TCTACACATC CATATGGCTG TCGTGTGATT CAGAGGATCC TCGAGCACTG TCTTCCTGAG
    CAGACTCTTC CCATCTTGGA GGAGCTTCAC CAGCACACTG AGCAGCTTGT GCAGGATCAG
    TATGGGAACT ATGTTATCCA GCATGTACTA GAGCACGGTC GGCCTGAGGA CAAGAGCAAG
    ATTGTAGCAG AAATTAGAGG CAACGTGCTC GTCTTGAGTC AACATAAATT TGCTAGCAAC
    GTTGTGGAGA AGTGTGTGAC CCACGCCTCC CGTACGGAGC GTGCCATGTT GATCGATGAG
    GTGTGCACTA TGAACGATGG CCCTCACAGT GCCTTATACA CCATGATGAA GGATCAGTAT
    GCCAACTACG TGGTACAGAA GATGATCGAT GTGGCAGAAC CAGCTCAGCG GAAGATTGTC
    ATGCACAAGA TCCGGCCCCA CATCGCCACC CTGCGCAAGT ACACCTACGG CAAGCACATC
    TTGGCCAAGC TGGAGAAGTA CTACATGAAG AACGGCGTTG ACCTCGGGCC CATCTGTGGA
    CCTCCAAATG GCATCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45502
    Clone ID Related Accession (Same CDS sequence) XM_015297539.1
    Accession Version XM_015297539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGCCCTGCA
    GTTGTCCCTC ACCAGTACTA CGGAGTTACA CCCTGGGGAG TTTATCCTGC CAGCCTCTTC
    CAGCAGCAAG CTGCAGCAGC TGCCGCTGCC ACTAACTCAG CAAATCAGCA GACCACTCAG
    CAAACCCAGC AGGGCCAGCA ACAGGTTCTA CGTGGCGGAG CCAGTCAGCG TCCTTTGACC
    CCAAACCAGA ACCAGCAGGG ACAGCAGACT GATCCGCTGG TGGCTGCCGC CGCCGTCAAC
    TCTGCCCTTG CCTTTGGACA AGGGCTGGCA GCAGGGATGC CAGGGTACCC TGTGCTGGCT
    CCTGCTGCTT ACTATGACCA AACTGGTGCT CTTGTGGTGA ATGCGGGGGC CAGGAATGGT
    CTGGGGGCAC CCGTGCGTCT GGTGGCCCCT GCACCAGTTA TCATCAGCTC CTCTGCAGCA
    CAGGCAGCAG TTGCAGCAGC GGCAGCTTCA GCCAACGGTG CTGCGGGGGG CCTGGCAGGA
    ACCACCAACG GCCCCTTCCG CCCCCTGGGA ACGCAGCAGC CCCAGCCTCA GCCGCAGCAG
    CAGCCCACCA ACAACTTGGC CTCCAGCTCG TTTTATGGCA ACAACTCACT CAGCAGCAAT
    TCCCAGAGCA GCTCCCTCTT CTCTCAGGGC TCTGCCCAGC CTGCCAACAC CTCCCTGGGC
    TTTGGGAGCA GCAGCTCCCT TGGTGCCACT CTGGGGTCTG CACTGGGAGG CTTTGGGACA
    GCAGTTGCTA ACTCCAACAC TGGCAGCGGC TCTCGCCGAG ATTCCCTGAC AGGGAGCAGT
    GACCTGTACA AGAGGACATC CAGCAGTTTG ACACCTATAG GACACAGTTT TTACAATGGC
    CTTGGGTTTT CCTCCTCTCC TGGACCTGTG GGTATGCCTC TGCCCAGCCA AGGACCTGGC
    CACTCTCAGA CTCCACCACC TTCCTTATCT TCACATGGAT CTTCTTCCAG TCTAAACCTG
    GGAGGGCTCA CAAATGGGAG CGGCCGTTAC ATCTCTGCTG CTCCTGGAGC TGAAGCCAAG
    TACCGCAGTG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACACT GTTCCCTTCG
    TCTCGCCTGC GCTATGGCAT GTCTGATGTT ATGCCCTCCG GCCGAAGCAG GCTGCTCGAA
    GACTTCCGCA ATAACCGGTA CCCCAATTTA CAGCTGAGGG AGATTGCTGG GCACATCATG
    GAGTTCTCCC AGGACCAGCA TGGATCCAGA TTTATTCAGC TGAAACTGGA GCGTGCTACC
    CCAGCAGAAC GTCAGCTTGT GTTCAACGAG ATCCTCCAGG CGGCTTATCA GTTGATGGTG
    GATGTATTTG GAAATTATGT CATCCAGAAG TTCTTTGAAT TTGGCAGCCT GGAGCAAAAG
    TTAGCCTTGG CAGAACGTAT CCGTGGGCAT GTTCTGTCCC TGGCTTTGCA GATGTATGGA
    TGTAGGGTGA TCCAGAAGGC CCTTGAGTTT ATTCCCCCAG ACCAGCAGGT AATTAACGAG
    ATGGTACGGG AGTTGGATGG CCATGTCCTG AAGTGTGTAA AAGACCAGAA CGGTAATCAT
    GTGGTGCAGA AGTGTATTGA GTGTGTGCAG CCTCAGTCCC TGCAGTTTAT CATTGATGCA
    TTTAAGGGAC AGGTTTTTGC GTTGTCTACA CATCCATATG GCTGTCGTGT GATTCAGAGG
    ATCCTCGAGC ACTGTCTTCC TGAGCAGACT CTTCCCATCT TGGAGGAGCT TCACCAGCAC
    ACTGAGCAGC TTGTGCAGGA TCAGTATGGG AACTATGTTA TCCAGCATGT ACTAGAGCAC
    GGTCGGCCTG AGGACAAGAG CAAGATTGTA GCAGAAATTA GAGGCAACGT GCTCGTCTTG
    AGTCAACATA AATTTGCTAG CAACGTTGTG GAGAAGTGTG TGACCCACGC CTCCCGTACG
    GAGCGTGCCA TGTTGATCGA TGAGGTGTGC ACTATGAACG ATGGCCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGATCA GTATGCCAAC TACGTGGTAC AGAAGATGAT CGATGTGGCA
    GAACCAGCTC AGCGGAAGAT TGTCATGCAC AAGATCCGGC CCCACATCGC CACCCTGCGC
    AAGTACACCT ACGGCAAGCA CATCTTGGCC AAGCTGGAGA AGTACTACAT GAAGAACGGC
    GTTGACCTCG GGCCCATCTG TGGACCTCCA AATGGCATCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153025.1
    CDS121..3585
    Translation

    Target ORF information:

    RefSeq Version XM_015297539.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGCCCTGCA
    GTTGTCCCTC ACCAGTACTA CGGAGTTACA CCCTGGGGAG TTTATCCTGC CAGCCTCTTC
    CAGCAGCAAG CTGCAGCAGC TGCCGCTGCC ACTAACTCAG CAAATCAGCA GACCACTCAG
    CAAACCCAGC AGGGCCAGCA ACAGGTTCTA CGTGGCGGAG CCAGTCAGCG TCCTTTGACC
    CCAAACCAGA ACCAGCAGGG ACAGCAGACT GATCCGCTGG TGGCTGCCGC CGCCGTCAAC
    TCTGCCCTTG CCTTTGGACA AGGGCTGGCA GCAGGGATGC CAGGGTACCC TGTGCTGGCT
    CCTGCTGCTT ACTATGACCA AACTGGTGCT CTTGTGGTGA ATGCGGGGGC CAGGAATGGT
    CTGGGGGCAC CCGTGCGTCT GGTGGCCCCT GCACCAGTTA TCATCAGCTC CTCTGCAGCA
    CAGGCAGCAG TTGCAGCAGC GGCAGCTTCA GCCAACGGTG CTGCGGGGGG CCTGGCAGGA
    ACCACCAACG GCCCCTTCCG CCCCCTGGGA ACGCAGCAGC CCCAGCCTCA GCCGCAGCAG
    CAGCCCACCA ACAACTTGGC CTCCAGCTCG TTTTATGGCA ACAACTCACT CAGCAGCAAT
    TCCCAGAGCA GCTCCCTCTT CTCTCAGGGC TCTGCCCAGC CTGCCAACAC CTCCCTGGGC
    TTTGGGAGCA GCAGCTCCCT TGGTGCCACT CTGGGGTCTG CACTGGGAGG CTTTGGGACA
    GCAGTTGCTA ACTCCAACAC TGGCAGCGGC TCTCGCCGAG ATTCCCTGAC AGGGAGCAGT
    GACCTGTACA AGAGGACATC CAGCAGTTTG ACACCTATAG GACACAGTTT TTACAATGGC
    CTTGGGTTTT CCTCCTCTCC TGGACCTGTG GGTATGCCTC TGCCCAGCCA AGGACCTGGC
    CACTCTCAGA CTCCACCACC TTCCTTATCT TCACATGGAT CTTCTTCCAG TCTAAACCTG
    GGAGGGCTCA CAAATGGGAG CGGCCGTTAC ATCTCTGCTG CTCCTGGAGC TGAAGCCAAG
    TACCGCAGTG CAAGCAGTGC CTCCAGCCTC TTCAGCCCCA GCAGCACACT GTTCCCTTCG
    TCTCGCCTGC GCTATGGCAT GTCTGATGTT ATGCCCTCCG GCCGAAGCAG GCTGCTCGAA
    GACTTCCGCA ATAACCGGTA CCCCAATTTA CAGCTGAGGG AGATTGCTGG GCACATCATG
    GAGTTCTCCC AGGACCAGCA TGGATCCAGA TTTATTCAGC TGAAACTGGA GCGTGCTACC
    CCAGCAGAAC GTCAGCTTGT GTTCAACGAG ATCCTCCAGG CGGCTTATCA GTTGATGGTG
    GATGTATTTG GAAATTATGT CATCCAGAAG TTCTTTGAAT TTGGCAGCCT GGAGCAAAAG
    TTAGCCTTGG CAGAACGTAT CCGTGGGCAT GTTCTGTCCC TGGCTTTGCA GATGTATGGA
    TGTAGGGTGA TCCAGAAGGC CCTTGAGTTT ATTCCCCCAG ACCAGCAGGT AATTAACGAG
    ATGGTACGGG AGTTGGATGG CCATGTCCTG AAGTGTGTAA AAGACCAGAA CGGTAATCAT
    GTGGTGCAGA AGTGTATTGA GTGTGTGCAG CCTCAGTCCC TGCAGTTTAT CATTGATGCA
    TTTAAGGGAC AGGTTTTTGC GTTGTCTACA CATCCATATG GCTGTCGTGT GATTCAGAGG
    ATCCTCGAGC ACTGTCTTCC TGAGCAGACT CTTCCCATCT TGGAGGAGCT TCACCAGCAC
    ACTGAGCAGC TTGTGCAGGA TCAGTATGGG AACTATGTTA TCCAGCATGT ACTAGAGCAC
    GGTCGGCCTG AGGACAAGAG CAAGATTGTA GCAGAAATTA GAGGCAACGT GCTCGTCTTG
    AGTCAACATA AATTTGCTAG CAACGTTGTG GAGAAGTGTG TGACCCACGC CTCCCGTACG
    GAGCGTGCCA TGTTGATCGA TGAGGTGTGC ACTATGAACG ATGGCCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGATCA GTATGCCAAC TACGTGGTAC AGAAGATGAT CGATGTGGCA
    GAACCAGCTC AGCGGAAGAT TGTCATGCAC AAGATCCGGC CCCACATCGC CACCCTGCGC
    AAGTACACCT ACGGCAAGCA CATCTTGGCC AAGCTGGAGA AGTACTACAT GAAGAACGGC
    GTTGACCTCG GGCCCATCTG TGGACCTCCA AATGGCATCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45503
    Clone ID Related Accession (Same CDS sequence) XM_015297540.2 , XM_015297540.1
    Accession Version XM_015297540.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAAAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153026.1
    CDS60..3506
    Translation

    Target ORF information:

    RefSeq Version XM_015297540.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAAAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45503
    Clone ID Related Accession (Same CDS sequence) XM_015297540.2 , XM_015297540.1
    Accession Version XM_015297540.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAAAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153026.1
    CDS121..3567
    Translation

    Target ORF information:

    RefSeq Version XM_015297540.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGCAGCTGTT GCCGGGTAAA AAGTTTTGGG AATCTGATGA TTCCAGCAAA
    GATGGACCAA AAGGGATATT TCTGGGAGAT CAGTGGAGAG ACAGTGCTTG GGGAACATCA
    GATCACTCTG TTTCCCAACC AATTATGGTT CAGAGAAGAC CTGGTCAGGG CTTTCATGTG
    AATAGTGAAG TCAACTCAGT GCTTTCACCA CGGTCAGAGA GTGGAGGACT TGGAGTTAGC
    ATGGTGGAAT ATGTGTTGAG CTCATCTCCC GGAGATTCCT GCCTAAGGAA AGGAGGATTT
    GGGCCAAGGG ATGCAGAGAA TGATGAGAAT GACAAAGGGG ATAAGAAAAA TAAGGGCACA
    TTTGATGGCG ATAAATTAGG AGATCTGAAG GAGGAGGGGG ATGTGATGGA TAAAACAAAT
    GGTTTGCCTG TGCAGAATGG AATCGACACA GATGTCAAAG ACTTCAGCCG TACACCTGGT
    AATTGCCAGA ACTCTGCTAG TGAAGTTGAT CTTCTGGGTC CAAACCAGAA TGGATCTGAG
    GGCTTAGCCC AGCTGGCGAG TACTAATGGT GCCAAGCCCG TGGAGGATTT TTCCAACATA
    GAGTCACAGA GTGTTCCCCT GGATCCCATG GAGCACGTTG GCATGGAGCC TCTGCAGTTC
    GATTATTCTG GCACCCAGGT ACCTGTGGAC TCAGCCGCAG CCACCGTGGG GCTCTTCGAT
    TACAATTCCC AACAGCAGTT GTTCCAAAGA CCGAATGCAC TTGCTGTTCA GCAGCTAACA
    GCAGCCCAGC AGCAGCAGTA TGCATTGGCA GCTGCCCATC AGCCTCACAT AGGTATGTTT
    TCAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCA GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCCCCTGCA CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTTGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGCGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACAAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTT
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAAAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45504
    Clone ID Related Accession (Same CDS sequence) XM_015297541.2 , XM_015297541.1
    Accession Version XM_015297541.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CTGTTGCCGG GTAAAAAGTT TTGGGAATCT GATGATTCCA GCAAAGATGG ACCAAAAGGG
    ATATTTCTGG GAGATCAGTG GAGAGACAGT GCTTGGGGAA CATCAGATCA CTCTGTTTCC
    CAACCAATTA TGGTTCAGAG AAGACCTGGT CAGGGCTTTC ATGTGAATAG TGAAGTCAAC
    TCAGTGCTTT CACCACGGTC AGAGAGTGGA GGACTTGGAG TTAGCATGGT GGAATATGTG
    TTGAGCTCAT CTCCCGGAGA TTCCTGCCTA AGGAAAGGAG GATTTGGGCC AAGGGATGCA
    GAGAATGATG AGAATGACAA AGGGGATAAG AAAAATAAGG GCACATTTGA TGGCGATAAA
    TTAGGAGATC TGAAGGAGGA GGGGGATGTG ATGGATAAAA CAAATGGTTT GCCTGTGCAG
    AATGGAATCG ACACAGATGT CAAAGACTTC AGCCGTACAC CTGGTAATTG CCAGAACTCT
    GCTAGTGAAG TTGATCTTCT GGGTCCAAAC CAGAATGGAT CTGAGGGCTT AGCCCAGCTG
    GCGAGTACTA ATGGTGCCAA GCCCGTGGAG GATTTTTCCA ACATAGAGTC ACAGAGTGTT
    CCCCTGGATC CCATGGAGCA CGTTGGCATG GAGCCTCTGC AGTTCGATTA TTCTGGCACC
    CAGGTACCTG TGGACTCAGC CGCAGCCACC GTGGGGCTCT TCGATTACAA TTCCCAACAG
    CAGTTGTTCC AAAGACCGAA TGCACTTGCT GTTCAGCAGC TAACAGCAGC CCAGCAGCAG
    CAGTATGCAT TGGCAGCTGC CCATCAGCCT CACATAGGTA TGTTTTCAGC AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153027.1
    CDS116..3322
    Translation

    Target ORF information:

    RefSeq Version XM_015297541.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297541.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CTGTTGCCGG GTAAAAAGTT TTGGGAATCT GATGATTCCA GCAAAGATGG ACCAAAAGGG
    ATATTTCTGG GAGATCAGTG GAGAGACAGT GCTTGGGGAA CATCAGATCA CTCTGTTTCC
    CAACCAATTA TGGTTCAGAG AAGACCTGGT CAGGGCTTTC ATGTGAATAG TGAAGTCAAC
    TCAGTGCTTT CACCACGGTC AGAGAGTGGA GGACTTGGAG TTAGCATGGT GGAATATGTG
    TTGAGCTCAT CTCCCGGAGA TTCCTGCCTA AGGAAAGGAG GATTTGGGCC AAGGGATGCA
    GAGAATGATG AGAATGACAA AGGGGATAAG AAAAATAAGG GCACATTTGA TGGCGATAAA
    TTAGGAGATC TGAAGGAGGA GGGGGATGTG ATGGATAAAA CAAATGGTTT GCCTGTGCAG
    AATGGAATCG ACACAGATGT CAAAGACTTC AGCCGTACAC CTGGTAATTG CCAGAACTCT
    GCTAGTGAAG TTGATCTTCT GGGTCCAAAC CAGAATGGAT CTGAGGGCTT AGCCCAGCTG
    GCGAGTACTA ATGGTGCCAA GCCCGTGGAG GATTTTTCCA ACATAGAGTC ACAGAGTGTT
    CCCCTGGATC CCATGGAGCA CGTTGGCATG GAGCCTCTGC AGTTCGATTA TTCTGGCACC
    CAGGTACCTG TGGACTCAGC CGCAGCCACC GTGGGGCTCT TCGATTACAA TTCCCAACAG
    CAGTTGTTCC AAAGACCGAA TGCACTTGCT GTTCAGCAGC TAACAGCAGC CCAGCAGCAG
    CAGTATGCAT TGGCAGCTGC CCATCAGCCT CACATAGGTA TGTTTTCAGC AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45504
    Clone ID Related Accession (Same CDS sequence) XM_015297541.2 , XM_015297541.1
    Accession Version XM_015297541.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CTGTTGCCGG GTAAAAAGTT TTGGGAATCT GATGATTCCA GCAAAGATGG ACCAAAAGGG
    ATATTTCTGG GAGATCAGTG GAGAGACAGT GCTTGGGGAA CATCAGATCA CTCTGTTTCC
    CAACCAATTA TGGTTCAGAG AAGACCTGGT CAGGGCTTTC ATGTGAATAG TGAAGTCAAC
    TCAGTGCTTT CACCACGGTC AGAGAGTGGA GGACTTGGAG TTAGCATGGT GGAATATGTG
    TTGAGCTCAT CTCCCGGAGA TTCCTGCCTA AGGAAAGGAG GATTTGGGCC AAGGGATGCA
    GAGAATGATG AGAATGACAA AGGGGATAAG AAAAATAAGG GCACATTTGA TGGCGATAAA
    TTAGGAGATC TGAAGGAGGA GGGGGATGTG ATGGATAAAA CAAATGGTTT GCCTGTGCAG
    AATGGAATCG ACACAGATGT CAAAGACTTC AGCCGTACAC CTGGTAATTG CCAGAACTCT
    GCTAGTGAAG TTGATCTTCT GGGTCCAAAC CAGAATGGAT CTGAGGGCTT AGCCCAGCTG
    GCGAGTACTA ATGGTGCCAA GCCCGTGGAG GATTTTTCCA ACATAGAGTC ACAGAGTGTT
    CCCCTGGATC CCATGGAGCA CGTTGGCATG GAGCCTCTGC AGTTCGATTA TTCTGGCACC
    CAGGTACCTG TGGACTCAGC CGCAGCCACC GTGGGGCTCT TCGATTACAA TTCCCAACAG
    CAGTTGTTCC AAAGACCGAA TGCACTTGCT GTTCAGCAGC TAACAGCAGC CCAGCAGCAG
    CAGTATGCAT TGGCAGCTGC CCATCAGCCT CACATAGGTA TGTTTTCAGC AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153027.1
    CDS112..3318
    Translation

    Target ORF information:

    RefSeq Version XM_015297541.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CTGTTGCCGG GTAAAAAGTT TTGGGAATCT GATGATTCCA GCAAAGATGG ACCAAAAGGG
    ATATTTCTGG GAGATCAGTG GAGAGACAGT GCTTGGGGAA CATCAGATCA CTCTGTTTCC
    CAACCAATTA TGGTTCAGAG AAGACCTGGT CAGGGCTTTC ATGTGAATAG TGAAGTCAAC
    TCAGTGCTTT CACCACGGTC AGAGAGTGGA GGACTTGGAG TTAGCATGGT GGAATATGTG
    TTGAGCTCAT CTCCCGGAGA TTCCTGCCTA AGGAAAGGAG GATTTGGGCC AAGGGATGCA
    GAGAATGATG AGAATGACAA AGGGGATAAG AAAAATAAGG GCACATTTGA TGGCGATAAA
    TTAGGAGATC TGAAGGAGGA GGGGGATGTG ATGGATAAAA CAAATGGTTT GCCTGTGCAG
    AATGGAATCG ACACAGATGT CAAAGACTTC AGCCGTACAC CTGGTAATTG CCAGAACTCT
    GCTAGTGAAG TTGATCTTCT GGGTCCAAAC CAGAATGGAT CTGAGGGCTT AGCCCAGCTG
    GCGAGTACTA ATGGTGCCAA GCCCGTGGAG GATTTTTCCA ACATAGAGTC ACAGAGTGTT
    CCCCTGGATC CCATGGAGCA CGTTGGCATG GAGCCTCTGC AGTTCGATTA TTCTGGCACC
    CAGGTACCTG TGGACTCAGC CGCAGCCACC GTGGGGCTCT TCGATTACAA TTCCCAACAG
    CAGTTGTTCC AAAGACCGAA TGCACTTGCT GTTCAGCAGC TAACAGCAGC CCAGCAGCAG
    CAGTATGCAT TGGCAGCTGC CCATCAGCCT CACATAGGTA TGTTTTCAGC AGGTTTAGCT
    CCTGCTGCCT TTGTCCCCAA TCCGTACATC ATCAGCGCTG CTCCACCAGG AACGGATCCG
    TACGCAGCCG GGCTCGCAGC AGCTGCCACA TTAGGCCCTG CAGTTGTCCC TCACCAGTAC
    TACGGAGTTA CACCCTGGGG AGTTTATCCT GCCAGCCTCT TCCAGCAGCA AGCTGCAGCA
    GCTGCCGCTG CCACTAACTC AGCAAATCAG CAGACCACTC AGCAAACCCA GCAGGGCCAG
    CAACAGGTTC TACGTGGCGG AGCCAGTCAG CGTCCTTTGA CCCCAAACCA GAACCAGCAG
    GGACAGCAGA CTGATCCGCT GGTGGCTGCC GCCGCCGTCA ACTCTGCCCT TGCCTTTGGA
    CAAGGGCTGG CAGCAGGGAT GCCAGGGTAC CCTGTGCTGG CTCCTGCTGC TTACTATGAC
    CAAACTGGTG CTCTTGTGGT GAATGCGGGG GCCAGGAATG GTCTGGGGGC ACCCGTGCGT
    CTGGTGGCCC CTGCACCAGT TATCATCAGC TCCTCTGCAG CACAGGCAGC AGTTGCAGCA
    GCGGCAGCTT CAGCCAACGG TGCTGCGGGG GGCCTGGCAG GAACCACCAA CGGCCCCTTC
    CGCCCCCTGG GAACGCAGCA GCCCCAGCCT CAGCCGCAGC AGCAGCCCAC CAACAACTTG
    GCCTCCAGCT CGTTTTATGG CAACAACTCA CTCAGCAGCA ATTCCCAGAG CAGCTCCCTC
    TTCTCTCAGG GCTCTGCCCA GCCTGCCAAC ACCTCCCTGG GCTTTGGGAG CAGCAGCTCC
    CTTGGTGCCA CTCTGGGGTC TGCACTGGGA GGCTTTGGGA CAGCAGTTGC TAACTCCAAC
    ACTGGCAGCG GCTCTCGCCG AGATTCCCTG ACAGGGAGCA GTGACCTGTA CAAGAGGACA
    TCCAGCAGTT TGACACCTAT AGGACACAGT TTTTACAATG GCCTTGGGTT TTCCTCCTCT
    CCTGGACCTG TGGGTATGCC TCTGCCCAGC CAAGGACCTG GCCACTCTCA GACTCCACCA
    CCTTCCTTAT CTTCACATGG ATCTTCTTCC AGTCTAAACC TGGGAGGGCT CACAAATGGG
    AGCGGCCGTT ACATCTCTGC TGCTCCTGGA GCTGAAGCCA AGTACCGCAG TGCAAGCAGT
    GCCTCCAGCC TCTTCAGCCC CAGCAGCACA CTGTTCCCTT CGTCTCGCCT GCGCTATGGC
    ATGTCTGATG TTATGCCCTC CGGCCGAAGC AGGCTGCTCG AAGACTTCCG CAATAACCGG
    TACCCCAATT TACAGCTGAG GGAGATTGCT GGGCACATCA TGGAGTTCTC CCAGGACCAG
    CATGGATCCA GATTTATTCA GCTGAAACTG GAGCGTGCTA CCCCAGCAGA ACGTCAGCTT
    GTGTTCAACG AGATCCTCCA GGCGGCTTAT CAGTTGATGG TGGATGTATT TGGAAATTAT
    GTCATCCAGA AGTTCTTTGA ATTTGGCAGC CTGGAGCAAA AGTTAGCCTT GGCAGAACGT
    ATCCGTGGGC ATGTTCTGTC CCTGGCTTTG CAGATGTATG GATGTAGGGT GATCCAGAAG
    GCCCTTGAGT TTATTCCCCC AGACCAGCAG GTAATTAACG AGATGGTACG GGAGTTGGAT
    GGCCATGTCC TGAAGTGTGT AAAAGACCAG AACGGTAATC ATGTGGTGCA GAAGTGTATT
    GAGTGTGTGC AGCCTCAGTC CCTGCAGTTT ATCATTGATG CATTTAAGGG ACAGGTTTTT
    GCGTTGTCTA CACATCCATA TGGCTGTCGT GTGATTCAGA GGATCCTCGA GCACTGTCTT
    CCTGAGCAGA CTCTTCCCAT CTTGGAGGAG CTTCACCAGC ACACTGAGCA GCTTGTGCAG
    GATCAGTATG GGAACTATGT TATCCAGCAT GTACTAGAGC ACGGTCGGCC TGAGGACAAG
    AGCAAGATTG TAGCAGAAAT TAGAGGCAAC GTGCTCGTCT TGAGTCAACA TAAATTTGCT
    AGCAACGTTG TGGAGAAGTG TGTGACCCAC GCCTCCCGTA CGGAGCGTGC CATGTTGATC
    GATGAGGTGT GCACTATGAA CGATGGCCCT CACAGTGCCT TATACACCAT GATGAAGGAT
    CAGTATGCCA ACTACGTGGT ACAGAAGATG ATCGATGTGG CAGAACCAGC TCAGCGGAAG
    ATTGTCATGC ACAAGATCCG GCCCCACATC GCCACCCTGC GCAAGTACAC CTACGGCAAG
    CACATCTTGG CCAAGCTGGA GAAGTACTAC ATGAAGAACG GCGTTGACCT CGGGCCCATC
    TGTGGACCTC CAAATGGCAT CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45505
    Clone ID Related Accession (Same CDS sequence) XM_015297542.1 , XM_015297542.2
    Accession Version XM_015297542.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456004.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CAGCTGTTGC CGGGTAAAAA GTTTTGGGAA TCTGATGATT CCAGCAAAGA TGGACCAAAA
    GGGATATTTC TGGGAGATCA GTGGAGAGAC AGTGCTTGGG GAACATCAGA TCACTCTGTT
    TCCCAACCAA TTATGGTTCA GAGAAGACCT GGTCAGGGCT TTCATGTGAA TAGTGAAGTC
    AACTCAGTGC TTTCACCACG GTCAGAGAGT GGAGGACTTG GAGTTAGCAT GGTGGAATAT
    GTGTTGAGCT CATCTCCCGG AGATTCCTGC CTAAGGAAAG GAGGATTTGG GCCAAGGGAT
    GCAGAGAATG ATGAGAATGA CAAAGGGGAT AAGAAAAATA AGGGCACATT TGATGGCGAT
    AAATTAGGAG ATCTGAAGGA GGAGGGGGAT GTGATGGATA AAACAAATGG TTTGCCTGTG
    CAGAATGGAA TCGACACAGA TGTCAAAGAC TTCAGCCGTA CACCTGGTAA TTGCCAGAAC
    TCTGCTAGTG AAGTTGATCT TCTGGGTCCA AACCAGAATG GATCTGAGGG CTTAGCCCAG
    CTGGCGAGTA CTAATGGTGC CAAGCCCGTG GAGGATTTTT CCAACATAGA GTCACAGAGT
    GTTCCCCTGG ATCCCATGGA GCACGTTGGC ATGGAGCCTC TGCAGTTCGA TTATTCTGGC
    ACCCAGGTAC CTGTGGACTC AGCCGCAGCC ACCGTGGGGC TCTTCGATTA CAATTCCCAA
    CAGCAGTTGT TCCAAAGACC GAATGCACTT GCTGTTCAGC AGCTAACAGC AGCCCAGCAG
    CAGCAGTATG CATTGGCAGC TGCCCATCAG CCTCACATAG GTATGTTTTC AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153028.1
    CDS213..3422
    Translation

    Target ORF information:

    RefSeq Version XM_015297542.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA-binding family member 1 (PUM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CAGCTGTTGC CGGGTAAAAA GTTTTGGGAA TCTGATGATT CCAGCAAAGA TGGACCAAAA
    GGGATATTTC TGGGAGATCA GTGGAGAGAC AGTGCTTGGG GAACATCAGA TCACTCTGTT
    TCCCAACCAA TTATGGTTCA GAGAAGACCT GGTCAGGGCT TTCATGTGAA TAGTGAAGTC
    AACTCAGTGC TTTCACCACG GTCAGAGAGT GGAGGACTTG GAGTTAGCAT GGTGGAATAT
    GTGTTGAGCT CATCTCCCGG AGATTCCTGC CTAAGGAAAG GAGGATTTGG GCCAAGGGAT
    GCAGAGAATG ATGAGAATGA CAAAGGGGAT AAGAAAAATA AGGGCACATT TGATGGCGAT
    AAATTAGGAG ATCTGAAGGA GGAGGGGGAT GTGATGGATA AAACAAATGG TTTGCCTGTG
    CAGAATGGAA TCGACACAGA TGTCAAAGAC TTCAGCCGTA CACCTGGTAA TTGCCAGAAC
    TCTGCTAGTG AAGTTGATCT TCTGGGTCCA AACCAGAATG GATCTGAGGG CTTAGCCCAG
    CTGGCGAGTA CTAATGGTGC CAAGCCCGTG GAGGATTTTT CCAACATAGA GTCACAGAGT
    GTTCCCCTGG ATCCCATGGA GCACGTTGGC ATGGAGCCTC TGCAGTTCGA TTATTCTGGC
    ACCCAGGTAC CTGTGGACTC AGCCGCAGCC ACCGTGGGGC TCTTCGATTA CAATTCCCAA
    CAGCAGTTGT TCCAAAGACC GAATGCACTT GCTGTTCAGC AGCTAACAGC AGCCCAGCAG
    CAGCAGTATG CATTGGCAGC TGCCCATCAG CCTCACATAG GTATGTTTTC AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45505
    Clone ID Related Accession (Same CDS sequence) XM_015297542.1 , XM_015297542.2
    Accession Version XM_015297542.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006110.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CAGCTGTTGC CGGGTAAAAA GTTTTGGGAA TCTGATGATT CCAGCAAAGA TGGACCAAAA
    GGGATATTTC TGGGAGATCA GTGGAGAGAC AGTGCTTGGG GAACATCAGA TCACTCTGTT
    TCCCAACCAA TTATGGTTCA GAGAAGACCT GGTCAGGGCT TTCATGTGAA TAGTGAAGTC
    AACTCAGTGC TTTCACCACG GTCAGAGAGT GGAGGACTTG GAGTTAGCAT GGTGGAATAT
    GTGTTGAGCT CATCTCCCGG AGATTCCTGC CTAAGGAAAG GAGGATTTGG GCCAAGGGAT
    GCAGAGAATG ATGAGAATGA CAAAGGGGAT AAGAAAAATA AGGGCACATT TGATGGCGAT
    AAATTAGGAG ATCTGAAGGA GGAGGGGGAT GTGATGGATA AAACAAATGG TTTGCCTGTG
    CAGAATGGAA TCGACACAGA TGTCAAAGAC TTCAGCCGTA CACCTGGTAA TTGCCAGAAC
    TCTGCTAGTG AAGTTGATCT TCTGGGTCCA AACCAGAATG GATCTGAGGG CTTAGCCCAG
    CTGGCGAGTA CTAATGGTGC CAAGCCCGTG GAGGATTTTT CCAACATAGA GTCACAGAGT
    GTTCCCCTGG ATCCCATGGA GCACGTTGGC ATGGAGCCTC TGCAGTTCGA TTATTCTGGC
    ACCCAGGTAC CTGTGGACTC AGCCGCAGCC ACCGTGGGGC TCTTCGATTA CAATTCCCAA
    CAGCAGTTGT TCCAAAGACC GAATGCACTT GCTGTTCAGC AGCTAACAGC AGCCCAGCAG
    CAGCAGTATG CATTGGCAGC TGCCCATCAG CCTCACATAG GTATGTTTTC AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153028.1
    CDS116..3325
    Translation

    Target ORF information:

    RefSeq Version XM_015297542.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015297542.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAGC TGAACCATGA TTTTCAAGCG CTTGCTCTGG AAGGACGGGC TATGGGAGAG 
    CAGCTGTTGC CGGGTAAAAA GTTTTGGGAA TCTGATGATT CCAGCAAAGA TGGACCAAAA
    GGGATATTTC TGGGAGATCA GTGGAGAGAC AGTGCTTGGG GAACATCAGA TCACTCTGTT
    TCCCAACCAA TTATGGTTCA GAGAAGACCT GGTCAGGGCT TTCATGTGAA TAGTGAAGTC
    AACTCAGTGC TTTCACCACG GTCAGAGAGT GGAGGACTTG GAGTTAGCAT GGTGGAATAT
    GTGTTGAGCT CATCTCCCGG AGATTCCTGC CTAAGGAAAG GAGGATTTGG GCCAAGGGAT
    GCAGAGAATG ATGAGAATGA CAAAGGGGAT AAGAAAAATA AGGGCACATT TGATGGCGAT
    AAATTAGGAG ATCTGAAGGA GGAGGGGGAT GTGATGGATA AAACAAATGG TTTGCCTGTG
    CAGAATGGAA TCGACACAGA TGTCAAAGAC TTCAGCCGTA CACCTGGTAA TTGCCAGAAC
    TCTGCTAGTG AAGTTGATCT TCTGGGTCCA AACCAGAATG GATCTGAGGG CTTAGCCCAG
    CTGGCGAGTA CTAATGGTGC CAAGCCCGTG GAGGATTTTT CCAACATAGA GTCACAGAGT
    GTTCCCCTGG ATCCCATGGA GCACGTTGGC ATGGAGCCTC TGCAGTTCGA TTATTCTGGC
    ACCCAGGTAC CTGTGGACTC AGCCGCAGCC ACCGTGGGGC TCTTCGATTA CAATTCCCAA
    CAGCAGTTGT TCCAAAGACC GAATGCACTT GCTGTTCAGC AGCTAACAGC AGCCCAGCAG
    CAGCAGTATG CATTGGCAGC TGCCCATCAG CCTCACATAG GTATGTTTTC AGCAGGTTTA
    GCTCCTGCTG CCTTTGTCCC CAATCCGTAC ATCATCAGCG CTGCTCCACC AGGAACGGAT
    CCGTACGCAG CCGGGCTCGC AGCAGCTGCC ACATTAGGCC CTGCAGTTGT CCCTCACCAG
    TACTACGGAG TTACACCCTG GGGAGTTTAT CCTGCCAGCC TCTTCCAGCA GCAAGCTGCA
    GCAGCTGCCG CTGCCACTAA CTCAGCAAAT CAGCAGACCA CTCAGCAAAC CCAGCAGGGC
    CAGCAACAGG TTCTACGTGG CGGAGCCAGT CAGCGTCCTT TGACCCCAAA CCAGAACCAG
    CAGGGACAGC AGACTGATCC GCTGGTGGCT GCCGCCGCCG TCAACTCTGC CCTTGCCTTT
    GGACAAGGGC TGGCAGCAGG GATGCCAGGG TACCCTGTGC TGGCTCCTGC TGCTTACTAT
    GACCAAACTG GTGCTCTTGT GGTGAATGCG GGGGCCAGGA ATGGTCTGGG GGCACCCGTG
    CGTCTGGTGG CCCCTGCACC AGTTATCATC AGCTCCTCTG CAGCACAGGC AGCAGTTGCA
    GCAGCGGCAG CTTCAGCCAA CGGTGCTGCG GGGGGCCTGG CAGGAACCAC CAACGGCCCC
    TTCCGCCCCC TGGGAACGCA GCAGCCCCAG CCTCAGCCGC AGCAGCAGCC CACCAACAAC
    TTGGCCTCCA GCTCGTTTTA TGGCAACAAC TCACTCAGCA GCAATTCCCA GAGCAGCTCC
    CTCTTCTCTC AGGGCTCTGC CCAGCCTGCC AACACCTCCC TGGGCTTTGG GAGCAGCAGC
    TCCCTTGGTG CCACTCTGGG GTCTGCACTG GGAGGCTTTG GGACAGCAGT TGCTAACTCC
    AACACTGGCA GCGGCTCTCG CCGAGATTCC CTGACAGGGA GCAGTGACCT GTACAAGAGG
    ACATCCAGCA GTTTGACACC TATAGGACAC AGTTTTTACA ATGGCCTTGG GTTTTCCTCC
    TCTCCTGGAC CTGTGGGTAT GCCTCTGCCC AGCCAAGGAC CTGGCCACTC TCAGACTCCA
    CCACCTTCCT TATCTTCACA TGGATCTTCT TCCAGTCTAA ACCTGGGAGG GCTCACAAAT
    GGGAGCGGCC GTTACATCTC TGCTGCTCCT GGAGCTGAAG CCAAGTACCG CAGTGCAAGC
    AGTGCCTCCA GCCTCTTCAG CCCCAGCAGC ACACTGTTCC CTTCGTCTCG CCTGCGCTAT
    GGCATGTCTG ATGTTATGCC CTCCGGCCGA AGCAGGCTGC TCGAAGACTT CCGCAATAAC
    CGGTACCCCA ATTTACAGCT GAGGGAGATT GCTGGGCACA TCATGGAGTT CTCCCAGGAC
    CAGCATGGAT CCAGATTTAT TCAGCTGAAA CTGGAGCGTG CTACCCCAGC AGAACGTCAG
    CTTGTGTTCA ACGAGATCCT CCAGGCGGCT TATCAGTTGA TGGTGGATGT ATTTGGAAAT
    TATGTCATCC AGAAGTTCTT TGAATTTGGC AGCCTGGAGC AAAAGTTAGC CTTGGCAGAA
    CGTATCCGTG GGCATGTTCT GTCCCTGGCT TTGCAGATGT ATGGATGTAG GGTGATCCAG
    AAGGCCCTTG AGTTTATTCC CCCAGACCAG CAGGTAATTA ACGAGATGGT ACGGGAGTTG
    GATGGCCATG TCCTGAAGTG TGTAAAAGAC CAGAACGGTA ATCATGTGGT GCAGAAGTGT
    ATTGAGTGTG TGCAGCCTCA GTCCCTGCAG TTTATCATTG ATGCATTTAA GGGACAGGTT
    TTTGCGTTGT CTACACATCC ATATGGCTGT CGTGTGATTC AGAGGATCCT CGAGCACTGT
    CTTCCTGAGC AGACTCTTCC CATCTTGGAG GAGCTTCACC AGCACACTGA GCAGCTTGTG
    CAGGATCAGT ATGGGAACTA TGTTATCCAG CATGTACTAG AGCACGGTCG GCCTGAGGAC
    AAGAGCAAGA TTGTAGCAGA AATTAGAGGC AACGTGCTCG TCTTGAGTCA ACATAAATTT
    GCTAGCAACG TTGTGGAGAA GTGTGTGACC CACGCCTCCC GTACGGAGCG TGCCATGTTG
    ATCGATGAGG TGTGCACTAT GAACGATGGC CCTCACAGTG CCTTATACAC CATGATGAAG
    GATCAGTATG CCAACTACGT GGTACAGAAG ATGATCGATG TGGCAGAACC AGCTCAGCGG
    AAGATTGTCA TGCACAAGAT CCGGCCCCAC ATCGCCACCC TGCGCAAGTA CACCTACGGC
    AAGCACATCT TGGCCAAGCT GGAGAAGTAC TACATGAAGA ACGGCGTTGA CCTCGGGCCC
    ATCTGTGGAC CTCCAAATGG CATCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00041
    Clone ID Related Accession (Same CDS sequence) NM_001012840.2
    Accession Version NM_001012840.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Gallus gallus(chicken)
    Product pumilio homolog 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ851393.1. On Aug 31, 2012 this sequence version replaced NM_001012840.1. ##Evidence-Data-START## Transcript exon combination :: AJ851393.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGGGGCCAAG GGATGCAGAG AATGATGAGA ATGACAAAGG GGATAAGAAA
    AATAAGGGCA CATTTGATGG CGATAAATTA GGAGATCTGA AGGAGGAGGG GGATGTGATG
    GATAAAACAA ATGGTTTGCC TGTGCAGAAT GGAATCGACA CAGATGTCAA AGACTTCAGC
    CGTACACCTG GTAATTGCCA GAACTCTGCT AGTGAAGTTG ATCTTCTGGG TCCAAACCAG
    AATGGATCTG AGGGCTTAGC CCAGCTGGCA AGTACTAATG GTGCCAAGCC CGTGGAGGAT
    TTTTCCAACA TAGAGTCACA GAGTGTTCCC CTGGATCCCA TGGAGCACGT TGGCATGGAG
    CCTCTGCAGT TTGATTATTC TGGCACCCAG GTACCTGTGG ACTCAGCCGC AGCCACCGTG
    GGGCTCTTCG ATTACAATTC CCAACAGCAG TTGTTCCAAA GACCGAATGC ACTTGCTGTT
    CAGCAGCTAA CAGCAGCCCA GCAGCAGCAG TATGCATTGG CAGCTGCCCA TCAGCCTCAC
    ATAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCC GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCTCCTGCG CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTCGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGTGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACGAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTC
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001012858.1
    CDS106..3387
    Translation

    Target ORF information:

    RefSeq Version NM_001012840.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus pumilio RNA binding family member 1 (PUM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001012840.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCGTTG CGTGTGTGTT GAAGAGAAAA GCAGTGCTCT GGCAGGACTC GTTCAGCCCC 
    CACCTGAAAC AGCACGCTCA AGATACAGCT AATCCCAACA TGCCTGTTGT ATTGACATCT
    GGAACAGGGT CCCAGGCTCA GCCACAGCCA GCTGCAAACC AGGCTCTTGC AGCAGGGACA
    CACTCCAGTC CTGTTCCTGG ATCCATAGGA GTTGCAGGCC GCTCCCAGGA CGACGCTATG
    GTGGATTACT TCTTTCAGAG GCAGCATGGT GAGCAGCTTG GGGGAGGAGG AAGTGGTGGA
    GGCGGCTATA ATAACAGCAA ACATCGCTGG CCTACTGGGG ATAACATTCA TGCAGAACAT
    CAGGTGCGTT CTATGGATGA GCTGAACCAT GATTTTCAAG CGCTTGCTCT GGAAGGACGG
    GCTATGGGAG AGGGGCCAAG GGATGCAGAG AATGATGAGA ATGACAAAGG GGATAAGAAA
    AATAAGGGCA CATTTGATGG CGATAAATTA GGAGATCTGA AGGAGGAGGG GGATGTGATG
    GATAAAACAA ATGGTTTGCC TGTGCAGAAT GGAATCGACA CAGATGTCAA AGACTTCAGC
    CGTACACCTG GTAATTGCCA GAACTCTGCT AGTGAAGTTG ATCTTCTGGG TCCAAACCAG
    AATGGATCTG AGGGCTTAGC CCAGCTGGCA AGTACTAATG GTGCCAAGCC CGTGGAGGAT
    TTTTCCAACA TAGAGTCACA GAGTGTTCCC CTGGATCCCA TGGAGCACGT TGGCATGGAG
    CCTCTGCAGT TTGATTATTC TGGCACCCAG GTACCTGTGG ACTCAGCCGC AGCCACCGTG
    GGGCTCTTCG ATTACAATTC CCAACAGCAG TTGTTCCAAA GACCGAATGC ACTTGCTGTT
    CAGCAGCTAA CAGCAGCCCA GCAGCAGCAG TATGCATTGG CAGCTGCCCA TCAGCCTCAC
    ATAGCAGGTT TAGCTCCTGC TGCCTTTGTC CCCAATCCGT ACATCATCAG CGCTGCTCCA
    CCAGGAACGG ATCCGTACGC AGCCGGGCTC GCAGCAGCTG CCACATTAGG CCCTGCAGTT
    GTCCCTCACC AGTACTACGG AGTTACACCC TGGGGAGTTT ATCCTGCCAG CCTCTTCCAG
    CAGCAAGCTG CAGCAGCTGC CGCTGCCACT AACTCAGCAA ATCAGCAGAC CACTCAGCAA
    ACCCAGCAGG GCCAGCAACA GGTTCTACGT GGCGGAGCCA GTCAGCGTCC TTTGACCCCA
    AACCAGAACC AGCAGGGACA GCAGACTGAT CCGCTGGTGG CTGCCGCCGC CGTCAACTCT
    GCCCTTGCCT TTGGACAAGG GCTGGCAGCC GGGATGCCAG GGTACCCTGT GCTGGCTCCT
    GCTGCTTACT ATGACCAAAC TGGTGCTCTT GTGGTGAATG CGGGGGCCAG GAATGGTCTG
    GGGGCACCCG TGCGTCTGGT GGCTCCTGCG CCAGTTATCA TCAGCTCCTC TGCAGCACAG
    GCAGCAGTTG CAGCAGCGGC AGCTTCAGCC AACGGTGCTG CGGGGGGCCT GGCAGGAACC
    ACCAACGGCC CCTTCCGCCC CCTGGGAACG CAGCAGCCCC AGCCTCAGCC GCAGCAGCAG
    CCCACCAACA ACTTGGCCTC CAGCTCGTTT TATGGCAACA ACTCACTCAG CAGCAATTCC
    CAGAGCAGCT CCCTCTTCTC TCAGGGCTCT GCCCAGCCTG CCAACACCTC CCTGGGCTTT
    GGGAGCAGCA GCTCCCTCGG TGCCACTCTG GGGTCTGCAC TGGGAGGCTT TGGGACAGCA
    GTTGCTAACT CCAACACTGG CAGTGGCTCT CGCCGAGATT CCCTGACAGG GAGCAGTGAC
    CTGTACGAGA GGACATCCAG CAGTTTGACA CCTATAGGAC ACAGTTTTTA CAATGGCCTC
    GGGTTTTCCT CCTCTCCTGG ACCTGTGGGT ATGCCTCTGC CCAGCCAAGG ACCTGGCCAC
    TCTCAGACTC CACCACCTTC CTTATCTTCA CATGGATCTT CTTCCAGTCT AAACCTGGGA
    GGGCTCACAA ATGGGAGCGG CCGTTACATC TCTGCTGCTC CTGGAGCTGA AGCCAAGTAC
    CGCAGTGCAA GCAGTGCCTC CAGCCTCTTC AGCCCCAGCA GCACACTGTT CCCTTCGTCT
    CGCCTGCGCT ATGGCATGTC TGATGTTATG CCCTCCGGCC GAAGCAGGCT GCTCGAAGAC
    TTCCGCAATA ACCGGTACCC CAATTTACAG CTGAGGGAGA TTGCTGGGCA CATCATGGAG
    TTCTCCCAGG ACCAGCATGG ATCCAGATTT ATTCAGCTGA AACTGGAGCG TGCTACCCCA
    GCAGAACGTC AGCTTGTGTT CAACGAGATC CTCCAGGCGG CTTATCAGTT GATGGTGGAT
    GTATTTGGAA ATTATGTCAT CCAGAAGTTC TTTGAATTTG GCAGCCTGGA GCAAAAGTTA
    GCCTTGGCAG AACGTATCCG TGGGCATGTT CTGTCCCTGG CTTTGCAGAT GTATGGATGT
    AGGGTGATCC AGAAGGCCCT TGAGTTTATT CCCCCAGACC AGCAGGTAAT TAACGAGATG
    GTACGGGAGT TGGATGGCCA TGTCCTGAAG TGTGTAAAAG ACCAGAACGG TAATCATGTG
    GTGCAGAAGT GTATTGAGTG TGTGCAGCCT CAGTCCCTGC AGTTTATCAT TGATGCATTT
    AAGGGACAGG TTTTTGCGTT GTCTACACAT CCATATGGCT GTCGTGTGAT TCAGAGGATC
    CTCGAGCACT GTCTTCCTGA GCAGACTCTT CCCATCTTGG AGGAGCTTCA CCAGCACACT
    GAGCAGCTTG TGCAGGATCA GTATGGGAAC TATGTTATCC AGCATGTACT AGAGCACGGT
    CGGCCTGAGG ACAAGAGCAA GATTGTAGCA GAAATTAGAG GCAACGTGCT CGTCTTGAGT
    CAACATAAAT TTGCTAGCAA CGTTGTGGAG AAGTGTGTGA CCCACGCCTC CCGTACGGAG
    CGTGCCATGT TGATCGATGA GGTGTGCACT ATGAACGATG GCCCTCACAG TGCCTTATAC
    ACCATGATGA AGGATCAGTA TGCCAACTAC GTGGTACAGA AGATGATCGA TGTGGCAGAA
    CCAGCTCAGC GGAAGATTGT CATGCACAAG ATCCGGCCCC ACATCGCCAC CCTGCGCAAG
    TACACCTACG GCAAGCACAT CTTGGCCAAG CTGGAGAAGT ACTACATGAA GAACGGCGTT
    GACCTCGGGC CCATCTGTGG ACCTCCAAAT GGCATCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.