C21H1ORF222 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	C21H1ORF222
Entrez Gene ID	419400
Full Name	cilia and flagella associated protein 74
Synonyms	C1orf222,C21H1ORF222
General protein information	Preferred Names cilia and flagella associated protein 74 Names cilia- and flagella-associated protein 74
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015297103.1	XP_015152589.1	cilia- and flagella-associated protein 74
XM_015297101.1	XP_015152587.1	cilia- and flagella-associated protein 74
XM_015297102.1	XP_015152588.1	cilia- and flagella-associated protein 74
XM_025142597.1	XP_024998365.1	cilia- and flagella-associated protein 74 isoform X2
XM_004947389.2	XP_004947446.1	cilia- and flagella-associated protein 74
XM_015297100.1	XP_015152586.1	cilia- and flagella-associated protein 74
XM_025142596.1	XP_024998364.1	cilia- and flagella-associated protein 74 isoform X1
XM_004947391.2	XP_004947448.1	cilia- and flagella-associated protein 74

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KIAA1751	XP_417562.4
Danio rerio (zebrafish)	si:dkeyp-87a12.1	XP_002663289.3
Homo sapiens (human)	KIAA1751	NP_001073953.1
Rattus norvegicus (Norway rat)	LOC100362748	XP_006239669.1
Canis lupus familiaris (dog)	KIAA1751	XP_005620468.1
Mus musculus (house mouse)	2010015L04Rik	NP_808342.3
	LOC709689	XP_001098248.2
Xenopus tropicalis (tropical clawed frog)	c1orf222	NP_001184181.1

Gene Ontology

The following C21H1ORF222 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C21H1ORF222 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa147093	XM_025142597.1 Latest version!	Gallus gallus cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa18174	XM_015297100.1 Latest version!	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa18174	XM_015297101.1 Latest version!	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa18174	XM_004947391.2 Latest version!	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa18174	XM_004947389.2 Latest version!	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa18174	XM_015297103.1 Latest version!	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa18174	XM_015297102.1 Latest version!	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa18174	XM_025142596.1 Latest version!	Gallus gallus cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C21H1ORF222 ( XM_025142597.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998365.1 Gallus gallus cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa147093
Clone ID Related Accession (Same CDS sequence)	XM_025142597.1
Accession Version	XM_025142597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGT TCCGCCTACA GGCAATGCAC AGGCGCCTGT GCACTGAACT GGAGGATGAG 
AAGAGTTTTG AGTTGAAAAT TGCTTTGACA CTGAAAGAGA ACATGCTTGA GATGTGGCGG 
ATAGAAATGC AGCAGGGAAA ATATGAAATC CTCCACAAAC AATCTAAAAA AGATGAGGAG 
GAGCGAGAGA TGCAATACCA GGAACAAGCA GAAGTGCGGA TTCGGAGGGA AAAAATAGCA 
GCTCTGAAAG CAGAAGAAAA TCAACTGTCT TTAAAAAAAA AAGAAGAGAA AGCCCAGAAA 
GAATATGAAA AAAGGCATAA GAAAATGCTT GACGGTGCCA AAAGGAACCA TGACAAAGCA 
GTCTACTTCC TGAGAAAAAG CATGGCAAGA ATTCATGAGA AGAATGCAAA GGAAGAAGCG 
AAAACTCAGG AGCATATGGA GAGAAGGATA CAAGCTGTGC TCTCCCTGAA AAACAGCATA 
ACTTCCAATA GGGAAAAACT CCAGGCTCTC CAGGCTTGGA ACCAAGCAAT GGCAAAGAAA 
CAGGAGATGA CAATGAGGGA GGCTATCCTA GCAGAAGGAG GCAATGTTGC CAAGGAAATT 
TTTCTCCATA AACGTTTGCT ACAGCATGAA AAGGAGAAAC AAGCTTTTAT AGAACAGCAA 
AAATCGAGAA AAATTGAGAT TGTATCTAAA ATTTTGAAAG AAAAAGCTTC TATTTGCAAG 
CAGAAAAACC AGCAGTTGTG TACTAAGGCA ATTAAGAGTG GTGGTAAATG TAAGGATTCT 
TTACTGTGGA GAAAGAAAGC GTGGCGCTAT ATTGAGAAGA CCTGCAAACA TCCAGCTGGG 
GCACTGCCAC AGGAGTCGTG GTACTCTCCA TCAGTTTGTT CCCCAGCTGG TGAGAGAACT 
GCTTCTGTAG GAGAGTGTTC TGAAACAGTC CCCCATGATG TGCCCTGTGA AAGTGAGGAA 
GGTGAGAAGG AGAGGGACGA GATCCCAGCA GAACCAGAGC TCCCAGGACT ATTGAGTCAG 
GAATGTGACT TGCACAAGAT ATCCAAAGAA GAAATGGATA CAAAGCATCT GGACACAAAG 
ATAACGAAAA CAGTGATTTT AGAGGACAGA AAGGAGGAAT TCCCAACGAG AATTTTTAAT 
AAGGGAACAG TGTCTGGTCA CGAACATCAA GGAAGATCTT TCTATAGTAA ACCAAGTTGT 
ATTCATTTCA AGGATTTTGA TGTTGGCAAA ACCTACAAAA AGAAGATAGT TTTGATAAAT 
GCATCCTACA GCATTAACTA CTGCAAACCA GTGGGAATCA GCAAATGGCT GAAGGACTTC 
ATCAGCATTC AGTTTGACCC ACCTGGCAAA ATGTCTGCTG GAATGTCCTG TGAAGTGGGG 
ATAACCTTTA AGCCTGTGAT AAATGAAAAT CTGGAAGGAG AAGTCATGTT TATGGCCCAG 
ACAGGATTCT TCTCAGTTCC ATTGAAATGT ACAGCCAAGA CATGCATTCT GGCACTAGAT 
AAAGTGCTTG TTGACTTTGG CACTCATGTG GTAGGAGAGA CAATTTCACG AACTGTCAAC 
CTGACAAACA CTGGAGCTTT GGGAACGAGA TTCAAAGTTC AGACATTAGG AGGTGACAGC 
AGTAGATGTG GAGCAACAGC AGAATCTTCT ACTACCAGAA CAGTTACTCA ACTGTGCAGT 
GACTCTGCTC CTGAAAGGAA AGACAGCAAT AGTTCTGTAA CATCTGTGCT TGAAAAGAAA 
GAACAAACTT GTCTTGATGG TAGTGAAGAA CCAACCTGCT GTGTAGCCCA GGTGGAGCAG 
CAGAGGACTG AGACAAGTTC TACAGAACAA CTCACTCAAG ATGCATTCAA TTTCAGTTCA 
GATAAAGACA TGGACAATGC ACATGATTTA ATGGCATGCT CTCCTGAAGA AATACCAATT 
GAAATTATGC TGGGAAAGGT TACTGAAGGT GAGATTGGAC CCTTCAGCTC AGTGAAACTT 
CAGATTATAT TTATGCCATC TATCCCTGGG GACGTGCGGG CAGAGTTTGA GATCGTGTTT 
GACAACTCAG ACTGCAAACC ACTGTACTTC AGTGCCACTG GAGTTGCTAT TGATGTGCCA 
GTTTGGGTGC CCAATCCCAA TGTTGATTTA AAAATCTGTA TGTATGACCG ACTTTACCAG 
GACTGCATTG TCATCCGGAG CAGAGCAAAA ACTACGCTGA ATTTGAAGTT CGAGGTATGC 
AAAGAATTGA GCAAACACAT GAAGCTGCTC CCAAAAACAG GTTACATTCT ACCTCGATCA 
TCCTTCCGTG TGCAGCTGAA GTTTCTGCCC AGGCACTCCC TCCCCGGAGA CGCAGGGAGC 
TGTTTTAATG AAGAGACAGG TGTGCTGGAA GCTCCAGTGA CAATACAGAT TGCAGATAAT 
ACTAAACAAA TTAATTTTAC TGTCCATGCA ATTGTTACTA CTACTGACTT GGTGATCAGT 
CCAGCAGAAA TCAATTTTGG ATACTGCACC ATCTACGAAG CCGTGAGGAC AAATGTCACG 
CTCACAAACA AGTCCATCCT GCCACAGGAG TTTGGATTTG TGAGACTCCC AGAGTTTGTT 
GAAATTCAGC CCAATGATGG ATTTGGAGTT CTTCTTCCTC TTGAAAGTCT GACATTGCAC 
GTAATCTTTA AAGCCAACAA GGCAAAAGAG TACAGCTTTG AACTGACCTG CAAGTCAGAA 
GTTAATAGAC AATTCAAGTT GTCATGCAGA GCAGTTGGAG TCCACCCACC TCTTGAATTG 
TCTCATTCCT TAGTTCAGTT TGCTGCAACA GCTCTAAATG ATGTGTCTAC AGCAACACTG 
TATGTGATGA ATTCCCATGT GAGTGCCAAC ATCTTTACTC ATGCAGTCCC AAGAATTGGC 
AATGGGGAAG TTGCCCCTGT TGGACCCACT TCATTTGAAT TCTATGTGCC AGAAGACTGT 
CCTGTGACAA TCACACCTTC TGTTGGAACT GTGTTGCCTG GAAAGAAAAG CTTGATTCAA 
GTGTCCTTCA GACCAACATT GTCAGATCAG CTAATCAGAG AAGAGGCAGC TCAGAGGCTT 
TGTGGTGCAG CTGCAACAGG GGCAGAAATA CAAGAAATAA AGAAAGATGA AAAGACAGAG 
AGAAAGAAAT TAAGTGTTCC CCTTTCCAGA CGGCAGTCTT CCAGACAGGT TGTAAGGACT 
GGAAGCGTTA AATGCCTGAG TTCTTCGTAT AAATCTGAGC AACCAGAATG CAAAGAGATA 
AAGCCTGACT CAGAAGCTTA TATAGCAGCC CAGGCATTCC TGACAAGGAA TTTCAGAGGA 
AAGTTTGAAA AATACACCAT ACCATGCTTT GTTGCAAGTG GACATGTTGA CGAAAAGAGA 
GGCTCGGAAA GTCTAAGCTT CAGCCCTTGT AACACCTTGT ATTTGGAGCT GCATTGTCCA 
GCTGTAGCAC CATCTGTTGT GGTCACTTCA GATAACGGAA AAAACACGGT TGATTTTGGT 
GATGTGGCAG TAGGCCAAAC AATACTGAAG GAAACTACAA TACAAAATAT CTCCCCAGAA 
AACCTGGAAC TACGATTCTC AGTTCTGAAT CCCAGTGGTC CCTTCCTGTT GGTCAGACCA 
GTTAGGATGC TTGAGCCAGG TGAAATCAGA ACTCTCATCG TCTCTTTTTC TCCAAATAAG 
AACAAATTGT TCCTTGAAAT GCTGGACATC CGGACTGCAA AAAACAACTT GCCACTAAGG 
ATCACTGGTT ATGGGGTGGT GCCATCAACT GTTTGCTCTG TGGAAGTACT TGATATGGGC 
TATGTCCTAA CCGGAGAGAA GGCAACGTCC ACATTCAAGG TACAGAACAC TTCCACGCTG 
ACCCTGCGAT ACTCCATTCA TCTGGATTCA TTTTCATCCG CGAGGGACAA AGATCAGCAG 
AGGGTTCCAT CTTTTCTTAT GTCCTCTTTG CAAGGAGCAG AGACTGTTGA AACAGAGAAC 
TGTAATGGTG TAAGTGCATT CAGTGTCCTT CCGGCAGAAG GAGAAATTTG TGGTGGGGAA 
AGCCAGGACT TTATTGTTAC TTTCAGTCCT GAGCGTGAAG GTCTATACTA TTCTGAGTGT 
CTCAAGGTTG TGCTCTTTGG CAAGAAAACT GCCCATGTGA TCCAGCTTAA GGGGGCAGCA 
AGAGATCATC CCATGTTTGT GGAAGGTGGG ATTCCACTAG ATGTGCCCAT TGAGTCCTTG 
GCTGTAACGT TACCTCCGTC ACCAAAAAAA GCACTAAAAG AAGAGCGACA AGAACCAGTG 
AAGTCCATTG TCCTGCTTCT GGAGCACGTT GAAGGAGAGA ATGCCCTACC GCCAGCAGTG 
GCAGAACTGA AAGTTGGAGC TATACAGACA GCTCAGTTTG CATCTAAGAA GAACGTGGAG 
TTCAGTTTTG ATAACCTGTC ACACCTCCAG AAGAAGGGAT TTGCTATTGA ACCTGTAAAG 
GGAAAAGTTG ATCGTGGTCA TGTAAAATGC ATCAGTGTTT TTTGGGTGCC ACCTGCTGAC 
TTCGATGCCA GTGCCCCCCT GGTTGAGACA GCCTTCCTGA ACTTAAAAGG TGACATTAAG 
GAATCTTACC GAATCCTCTT TGTGGCAACA GCTGTATCTG CTCCCACTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998365.1
CDS	38..4651
Translation	MAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_025142597.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142597.1

ORF Insert Sequence:

ATGGCCGTGT TCCGCCTACA GGCAATGCAC AGGCGCCTGT GCACTGAACT GGAGGATGAG 
AAGAGTTTTG AGTTGAAAAT TGCTTTGACA CTGAAAGAGA ACATGCTTGA GATGTGGCGG 
ATAGAAATGC AGCAGGGAAA ATATGAAATC CTCCACAAAC AATCTAAAAA AGATGAGGAG 
GAGCGAGAGA TGCAATACCA GGAACAAGCA GAAGTGCGGA TTCGGAGGGA AAAAATAGCA 
GCTCTGAAAG CAGAAGAAAA TCAACTGTCT TTAAAAAAAA AAGAAGAGAA AGCCCAGAAA 
GAATATGAAA AAAGGCATAA GAAAATGCTT GACGGTGCCA AAAGGAACCA TGACAAAGCA 
GTCTACTTCC TGAGAAAAAG CATGGCAAGA ATTCATGAGA AGAATGCAAA GGAAGAAGCG 
AAAACTCAGG AGCATATGGA GAGAAGGATA CAAGCTGTGC TCTCCCTGAA AAACAGCATA 
ACTTCCAATA GGGAAAAACT CCAGGCTCTC CAGGCTTGGA ACCAAGCAAT GGCAAAGAAA 
CAGGAGATGA CAATGAGGGA GGCTATCCTA GCAGAAGGAG GCAATGTTGC CAAGGAAATT 
TTTCTCCATA AACGTTTGCT ACAGCATGAA AAGGAGAAAC AAGCTTTTAT AGAACAGCAA 
AAATCGAGAA AAATTGAGAT TGTATCTAAA ATTTTGAAAG AAAAAGCTTC TATTTGCAAG 
CAGAAAAACC AGCAGTTGTG TACTAAGGCA ATTAAGAGTG GTGGTAAATG TAAGGATTCT 
TTACTGTGGA GAAAGAAAGC GTGGCGCTAT ATTGAGAAGA CCTGCAAACA TCCAGCTGGG 
GCACTGCCAC AGGAGTCGTG GTACTCTCCA TCAGTTTGTT CCCCAGCTGG TGAGAGAACT 
GCTTCTGTAG GAGAGTGTTC TGAAACAGTC CCCCATGATG TGCCCTGTGA AAGTGAGGAA 
GGTGAGAAGG AGAGGGACGA GATCCCAGCA GAACCAGAGC TCCCAGGACT ATTGAGTCAG 
GAATGTGACT TGCACAAGAT ATCCAAAGAA GAAATGGATA CAAAGCATCT GGACACAAAG 
ATAACGAAAA CAGTGATTTT AGAGGACAGA AAGGAGGAAT TCCCAACGAG AATTTTTAAT 
AAGGGAACAG TGTCTGGTCA CGAACATCAA GGAAGATCTT TCTATAGTAA ACCAAGTTGT 
ATTCATTTCA AGGATTTTGA TGTTGGCAAA ACCTACAAAA AGAAGATAGT TTTGATAAAT 
GCATCCTACA GCATTAACTA CTGCAAACCA GTGGGAATCA GCAAATGGCT GAAGGACTTC 
ATCAGCATTC AGTTTGACCC ACCTGGCAAA ATGTCTGCTG GAATGTCCTG TGAAGTGGGG 
ATAACCTTTA AGCCTGTGAT AAATGAAAAT CTGGAAGGAG AAGTCATGTT TATGGCCCAG 
ACAGGATTCT TCTCAGTTCC ATTGAAATGT ACAGCCAAGA CATGCATTCT GGCACTAGAT 
AAAGTGCTTG TTGACTTTGG CACTCATGTG GTAGGAGAGA CAATTTCACG AACTGTCAAC 
CTGACAAACA CTGGAGCTTT GGGAACGAGA TTCAAAGTTC AGACATTAGG AGGTGACAGC 
AGTAGATGTG GAGCAACAGC AGAATCTTCT ACTACCAGAA CAGTTACTCA ACTGTGCAGT 
GACTCTGCTC CTGAAAGGAA AGACAGCAAT AGTTCTGTAA CATCTGTGCT TGAAAAGAAA 
GAACAAACTT GTCTTGATGG TAGTGAAGAA CCAACCTGCT GTGTAGCCCA GGTGGAGCAG 
CAGAGGACTG AGACAAGTTC TACAGAACAA CTCACTCAAG ATGCATTCAA TTTCAGTTCA 
GATAAAGACA TGGACAATGC ACATGATTTA ATGGCATGCT CTCCTGAAGA AATACCAATT 
GAAATTATGC TGGGAAAGGT TACTGAAGGT GAGATTGGAC CCTTCAGCTC AGTGAAACTT 
CAGATTATAT TTATGCCATC TATCCCTGGG GACGTGCGGG CAGAGTTTGA GATCGTGTTT 
GACAACTCAG ACTGCAAACC ACTGTACTTC AGTGCCACTG GAGTTGCTAT TGATGTGCCA 
GTTTGGGTGC CCAATCCCAA TGTTGATTTA AAAATCTGTA TGTATGACCG ACTTTACCAG 
GACTGCATTG TCATCCGGAG CAGAGCAAAA ACTACGCTGA ATTTGAAGTT CGAGGTATGC 
AAAGAATTGA GCAAACACAT GAAGCTGCTC CCAAAAACAG GTTACATTCT ACCTCGATCA 
TCCTTCCGTG TGCAGCTGAA GTTTCTGCCC AGGCACTCCC TCCCCGGAGA CGCAGGGAGC 
TGTTTTAATG AAGAGACAGG TGTGCTGGAA GCTCCAGTGA CAATACAGAT TGCAGATAAT 
ACTAAACAAA TTAATTTTAC TGTCCATGCA ATTGTTACTA CTACTGACTT GGTGATCAGT 
CCAGCAGAAA TCAATTTTGG ATACTGCACC ATCTACGAAG CCGTGAGGAC AAATGTCACG 
CTCACAAACA AGTCCATCCT GCCACAGGAG TTTGGATTTG TGAGACTCCC AGAGTTTGTT 
GAAATTCAGC CCAATGATGG ATTTGGAGTT CTTCTTCCTC TTGAAAGTCT GACATTGCAC 
GTAATCTTTA AAGCCAACAA GGCAAAAGAG TACAGCTTTG AACTGACCTG CAAGTCAGAA 
GTTAATAGAC AATTCAAGTT GTCATGCAGA GCAGTTGGAG TCCACCCACC TCTTGAATTG 
TCTCATTCCT TAGTTCAGTT TGCTGCAACA GCTCTAAATG ATGTGTCTAC AGCAACACTG 
TATGTGATGA ATTCCCATGT GAGTGCCAAC ATCTTTACTC ATGCAGTCCC AAGAATTGGC 
AATGGGGAAG TTGCCCCTGT TGGACCCACT TCATTTGAAT TCTATGTGCC AGAAGACTGT 
CCTGTGACAA TCACACCTTC TGTTGGAACT GTGTTGCCTG GAAAGAAAAG CTTGATTCAA 
GTGTCCTTCA GACCAACATT GTCAGATCAG CTAATCAGAG AAGAGGCAGC TCAGAGGCTT 
TGTGGTGCAG CTGCAACAGG GGCAGAAATA CAAGAAATAA AGAAAGATGA AAAGACAGAG 
AGAAAGAAAT TAAGTGTTCC CCTTTCCAGA CGGCAGTCTT CCAGACAGGT TGTAAGGACT 
GGAAGCGTTA AATGCCTGAG TTCTTCGTAT AAATCTGAGC AACCAGAATG CAAAGAGATA 
AAGCCTGACT CAGAAGCTTA TATAGCAGCC CAGGCATTCC TGACAAGGAA TTTCAGAGGA 
AAGTTTGAAA AATACACCAT ACCATGCTTT GTTGCAAGTG GACATGTTGA CGAAAAGAGA 
GGCTCGGAAA GTCTAAGCTT CAGCCCTTGT AACACCTTGT ATTTGGAGCT GCATTGTCCA 
GCTGTAGCAC CATCTGTTGT GGTCACTTCA GATAACGGAA AAAACACGGT TGATTTTGGT 
GATGTGGCAG TAGGCCAAAC AATACTGAAG GAAACTACAA TACAAAATAT CTCCCCAGAA 
AACCTGGAAC TACGATTCTC AGTTCTGAAT CCCAGTGGTC CCTTCCTGTT GGTCAGACCA 
GTTAGGATGC TTGAGCCAGG TGAAATCAGA ACTCTCATCG TCTCTTTTTC TCCAAATAAG 
AACAAATTGT TCCTTGAAAT GCTGGACATC CGGACTGCAA AAAACAACTT GCCACTAAGG 
ATCACTGGTT ATGGGGTGGT GCCATCAACT GTTTGCTCTG TGGAAGTACT TGATATGGGC 
TATGTCCTAA CCGGAGAGAA GGCAACGTCC ACATTCAAGG TACAGAACAC TTCCACGCTG 
ACCCTGCGAT ACTCCATTCA TCTGGATTCA TTTTCATCCG CGAGGGACAA AGATCAGCAG 
AGGGTTCCAT CTTTTCTTAT GTCCTCTTTG CAAGGAGCAG AGACTGTTGA AACAGAGAAC 
TGTAATGGTG TAAGTGCATT CAGTGTCCTT CCGGCAGAAG GAGAAATTTG TGGTGGGGAA 
AGCCAGGACT TTATTGTTAC TTTCAGTCCT GAGCGTGAAG GTCTATACTA TTCTGAGTGT 
CTCAAGGTTG TGCTCTTTGG CAAGAAAACT GCCCATGTGA TCCAGCTTAA GGGGGCAGCA 
AGAGATCATC CCATGTTTGT GGAAGGTGGG ATTCCACTAG ATGTGCCCAT TGAGTCCTTG 
GCTGTAACGT TACCTCCGTC ACCAAAAAAA GCACTAAAAG AAGAGCGACA AGAACCAGTG 
AAGTCCATTG TCCTGCTTCT GGAGCACGTT GAAGGAGAGA ATGCCCTACC GCCAGCAGTG 
GCAGAACTGA AAGTTGGAGC TATACAGACA GCTCAGTTTG CATCTAAGAA GAACGTGGAG 
TTCAGTTTTG ATAACCTGTC ACACCTCCAG AAGAAGGGAT TTGCTATTGA ACCTGTAAAG 
GGAAAAGTTG ATCGTGGTCA TGTAAAATGC ATCAGTGTTT TTTGGGTGCC ACCTGCTGAC 
TTCGATGCCA GTGCCCCCCT GGTTGAGACA GCCTTCCTGA ACTTAAAAGG TGACATTAAG 
GAATCTTACC GAATCCTCTT TGTGGCAACA GCTGTATCTG CTCCCACTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_015297103.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152589.1 Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_015297103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152589.1
CDS	333..5405
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_015297103.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297103.1

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_015297101.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152587.1 Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X3, mRNA.

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CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_015297101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152587.1
CDS	643..5715
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_015297101.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297101.1

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_015297102.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152588.1 Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X5, mRNA.

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CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_015297102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152588.1
CDS	333..5405
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_015297102.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297102.1

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_004947389.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947446.1 Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X4, mRNA.

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CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_004947389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004947389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947446.1
CDS	333..5405
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_004947389.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004947389.2

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_015297100.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152586.1 Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X1, mRNA.

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CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_015297100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152586.1
CDS	333..5405
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_015297100.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297100.1

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_025142596.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998364.1 Gallus gallus cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.

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CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_025142596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998364.1
CDS	175..5247
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_025142596.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142596.1

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C21H1ORF222 ( XM_004947391.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947448.1 Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X2, mRNA.

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CloneID	OGa18174
Clone ID Related Accession (Same CDS sequence)	XM_015297103.1 , XM_015297101.1 , XM_015297102.1 , XM_004947389.2 , XM_015297100.1 , XM_025142596.1 , XM_004947391.2
Accession Version	XM_004947391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	cilia- and flagella-associated protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004947391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947448.1
CDS	422..5494
Translation	MEGSSEPGSGGEEEPTLIPFLEEEGNGTAAQSSDPGEHEEEYKLEEDLSDEGTMGNEVTSEGDGDKSEPSMDSLEHGLDDVTSSQALKSADRICLFPLPRNLKELSDIAKEKELVVQKIRDELTACQLRIKALEKQLENVDIETEEEKEAGNVAAVFRLQAMHRRLCTELEDEKSFELKIALTLKENMLEMWRIEMQQGKYEILHKQSKKDEEEREMQYQEQAEVRIRREKIAALKAEENQLSLKKKEEKAQKEYEKRHKKMLDGAKRNHDKAVYFLRKSMARIHEKNAKEEAKTQEHMERRIQAVLSLKNSITSNREKLQALQAWNQAMAKKQEMTMREAILAEGGNVAKEIFLHKRLLQHEKEKQAFIEQQKSRKIEIVSKILKEKASICKQKNQQLCTKAIKSGGKCKDSLLWRKKAWRYIEKTCKHPAGALPQESWYSPSVCSPAGERTASVGECSETVPHDVPCESEEGEKERDEIPAEPELPGLLSQECDLHKISKEEMDTKHLDTKITKTVILEDRKEEFPTRIFNKGTVSGHEHQGRSFYSKPSCIHFKDFDVGKTYKKKIVLINASYSINYCKPVGISKWLKDFISIQFDPPGKMSAGMSCEVGITFKPVINENLEGEVMFMAQTGFFSVPLKCTAKTCILALDKVLVDFGTHVVGETISRTVNLTNTGALGTRFKVQTLGGDSSRCGATAESSTTRTVTQLCSDSAPERKDSNSSVTSVLEKKEQTCLDGSEEPTCCVAQVEQQRTETSSTEQLTQDAFNFSSDKDMDNAHDLMACSPEEIPIEIMLGKVTEGEIGPFSSVKLQIIFMPSIPGDVRAEFEIVFDNSDCKPLYFSATGVAIDVPVWVPNPNVDLKICMYDRLYQDCIVIRSRAKTTLNLKFEVCKELSKHMKLLPKTGYILPRSSFRVQLKFLPRHSLPGDAGSCFNEETGVLEAPVTIQIADNTKQINFTVHAIVTTTDLVISPAEINFGYCTIYEAVRTNVTLTNKSILPQEFGFVRLPEFVEIQPNDGFGVLLPLESLTLHVIFKANKAKEYSFELTCKSEVNRQFKLSCRAVGVHPPLELSHSLVQFAATALNDVSTATLYVMNSHVSANIFTHAVPRIGNGEVAPVGPTSFEFYVPEDCPVTITPSVGTVLPGKKSLIQVSFRPTLSDQLIREEAAQRLCGAAATGAEIQEIKKDEKTERKKLSVPLSRRQSSRQVVRTGSVKCLSSSYKSEQPECKEIKPDSEAYIAAQAFLTRNFRGKFEKYTIPCFVASGHVDEKRGSESLSFSPCNTLYLELHCPAVAPSVVVTSDNGKNTVDFGDVAVGQTILKETTIQNISPENLELRFSVLNPSGPFLLVRPVRMLEPGEIRTLIVSFSPNKNKLFLEMLDIRTAKNNLPLRITGYGVVPSTVCSVEVLDMGYVLTGEKATSTFKVQNTSTLTLRYSIHLDSFSSARDKDQQRVPSFLMSSLQGAETVETENCNGVSAFSVLPAEGEICGGESQDFIVTFSPEREGLYYSECLKVVLFGKKTAHVIQLKGAARDHPMFVEGGIPLDVPIESLAVTLPPSPKKALKEERQEPVKSIVLLLEHVEGENALPPAVAELKVGAIQTAQFASKKNVEFSFDNLSHLQKKGFAIEPVKGKVDRGHVKCISVFWVPPADFDASAPLVETAFLNLKGDIKESYRILFVATAVSAPTS

Target ORF information:

RefSeq Version	XM_004947391.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chromosome 21 open reading frame, human C1orf222 (C21H1ORF222), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004947391.2

ORF Insert Sequence:

ATGGAAGGCA GCTCTGAGCC TGGGAGTGGT GGTGAGGAGG AACCAACTCT CATCCCGTTT 
CTGGAAGAGG AAGGCAACGG CACAGCGGCT CAGAGCTCTG ACCCCGGAGA ACATGAGGAA 
GAATACAAGC TTGAAGAAGA TTTGTCTGAC GAGGGCACAA TGGGGAACGA GGTCACTTCT 
GAAGGAGATG GAGACAAGTC TGAACCTAGC ATGGACAGCT TGGAACACGG CTTGGACGAT 
GTTACAAGCA GCCAGGCTCT GAAGTCTGCA GATAGAATTT GCCTATTTCC TTTACCCAGG 
AACCTAAAAG AGCTCAGTGA CATCGCCAAA GAAAAAGAAC TTGTGGTCCA GAAAATCAGA 
GATGAGCTGA CTGCCTGCCA GCTGCGGATC AAAGCGCTAG AAAAGCAGCT GGAGAATGTA 
GACATTGAGA CAGAAGAGGA GAAGGAGGCT GGCAATGTTG CAGCCGTGTT CCGCCTACAG 
GCAATGCACA GGCGCCTGTG CACTGAACTG GAGGATGAGA AGAGTTTTGA GTTGAAAATT 
GCTTTGACAC TGAAAGAGAA CATGCTTGAG ATGTGGCGGA TAGAAATGCA GCAGGGAAAA 
TATGAAATCC TCCACAAACA ATCTAAAAAA GATGAGGAGG AGCGAGAGAT GCAATACCAG 
GAACAAGCAG AAGTGCGGAT TCGGAGGGAA AAAATAGCAG CTCTGAAAGC AGAAGAAAAT 
CAACTGTCTT TAAAAAAAAA AGAAGAGAAA GCCCAGAAAG AATATGAAAA AAGGCATAAG 
AAAATGCTTG ACGGTGCCAA AAGGAACCAT GACAAAGCAG TCTACTTCCT GAGAAAAAGC 
ATGGCAAGAA TTCATGAGAA GAATGCAAAG GAAGAAGCGA AAACTCAGGA GCATATGGAG 
AGAAGGATAC AAGCTGTGCT CTCCCTGAAA AACAGCATAA CTTCCAATAG GGAAAAACTC 
CAGGCTCTCC AGGCTTGGAA CCAAGCAATG GCAAAGAAAC AGGAGATGAC AATGAGGGAG 
GCTATCCTAG CAGAAGGAGG CAATGTTGCC AAGGAAATTT TTCTCCATAA ACGTTTGCTA 
CAGCATGAAA AGGAGAAACA AGCTTTTATA GAACAGCAAA AATCGAGAAA AATTGAGATT 
GTATCTAAAA TTTTGAAAGA AAAAGCTTCT ATTTGCAAGC AGAAAAACCA GCAGTTGTGT 
ACTAAGGCAA TTAAGAGTGG TGGTAAATGT AAGGATTCTT TACTGTGGAG AAAGAAAGCG 
TGGCGCTATA TTGAGAAGAC CTGCAAACAT CCAGCTGGGG CACTGCCACA GGAGTCGTGG 
TACTCTCCAT CAGTTTGTTC CCCAGCTGGT GAGAGAACTG CTTCTGTAGG AGAGTGTTCT 
GAAACAGTCC CCCATGATGT GCCCTGTGAA AGTGAGGAAG GTGAGAAGGA GAGGGACGAG 
ATCCCAGCAG AACCAGAGCT CCCAGGACTA TTGAGTCAGG AATGTGACTT GCACAAGATA 
TCCAAAGAAG AAATGGATAC AAAGCATCTG GACACAAAGA TAACGAAAAC AGTGATTTTA 
GAGGACAGAA AGGAGGAATT CCCAACGAGA ATTTTTAATA AGGGAACAGT GTCTGGTCAC 
GAACATCAAG GAAGATCTTT CTATAGTAAA CCAAGTTGTA TTCATTTCAA GGATTTTGAT 
GTTGGCAAAA CCTACAAAAA GAAGATAGTT TTGATAAATG CATCCTACAG CATTAACTAC 
TGCAAACCAG TGGGAATCAG CAAATGGCTG AAGGACTTCA TCAGCATTCA GTTTGACCCA 
CCTGGCAAAA TGTCTGCTGG AATGTCCTGT GAAGTGGGGA TAACCTTTAA GCCTGTGATA 
AATGAAAATC TGGAAGGAGA AGTCATGTTT ATGGCCCAGA CAGGATTCTT CTCAGTTCCA 
TTGAAATGTA CAGCCAAGAC ATGCATTCTG GCACTAGATA AAGTGCTTGT TGACTTTGGC 
ACTCATGTGG TAGGAGAGAC AATTTCACGA ACTGTCAACC TGACAAACAC TGGAGCTTTG 
GGAACGAGAT TCAAAGTTCA GACATTAGGA GGTGACAGCA GTAGATGTGG AGCAACAGCA 
GAATCTTCTA CTACCAGAAC AGTTACTCAA CTGTGCAGTG ACTCTGCTCC TGAAAGGAAA 
GACAGCAATA GTTCTGTAAC ATCTGTGCTT GAAAAGAAAG AACAAACTTG TCTTGATGGT 
AGTGAAGAAC CAACCTGCTG TGTAGCCCAG GTGGAGCAGC AGAGGACTGA GACAAGTTCT 
ACAGAACAAC TCACTCAAGA TGCATTCAAT TTCAGTTCAG ATAAAGACAT GGACAATGCA 
CATGATTTAA TGGCATGCTC TCCTGAAGAA ATACCAATTG AAATTATGCT GGGAAAGGTT 
ACTGAAGGTG AGATTGGACC CTTCAGCTCA GTGAAACTTC AGATTATATT TATGCCATCT 
ATCCCTGGGG ACGTGCGGGC AGAGTTTGAG ATCGTGTTTG ACAACTCAGA CTGCAAACCA 
CTGTACTTCA GTGCCACTGG AGTTGCTATT GATGTGCCAG TTTGGGTGCC CAATCCCAAT 
GTTGATTTAA AAATCTGTAT GTATGACCGA CTTTACCAGG ACTGCATTGT CATCCGGAGC 
AGAGCAAAAA CTACGCTGAA TTTGAAGTTC GAGGTATGCA AAGAATTGAG CAAACACATG 
AAGCTGCTCC CAAAAACAGG TTACATTCTA CCTCGATCAT CCTTCCGTGT GCAGCTGAAG 
TTTCTGCCCA GGCACTCCCT CCCCGGAGAC GCAGGGAGCT GTTTTAATGA AGAGACAGGT 
GTGCTGGAAG CTCCAGTGAC AATACAGATT GCAGATAATA CTAAACAAAT TAATTTTACT 
GTCCATGCAA TTGTTACTAC TACTGACTTG GTGATCAGTC CAGCAGAAAT CAATTTTGGA 
TACTGCACCA TCTACGAAGC CGTGAGGACA AATGTCACGC TCACAAACAA GTCCATCCTG 
CCACAGGAGT TTGGATTTGT GAGACTCCCA GAGTTTGTTG AAATTCAGCC CAATGATGGA 
TTTGGAGTTC TTCTTCCTCT TGAAAGTCTG ACATTGCACG TAATCTTTAA AGCCAACAAG 
GCAAAAGAGT ACAGCTTTGA ACTGACCTGC AAGTCAGAAG TTAATAGACA ATTCAAGTTG 
TCATGCAGAG CAGTTGGAGT CCACCCACCT CTTGAATTGT CTCATTCCTT AGTTCAGTTT 
GCTGCAACAG CTCTAAATGA TGTGTCTACA GCAACACTGT ATGTGATGAA TTCCCATGTG 
AGTGCCAACA TCTTTACTCA TGCAGTCCCA AGAATTGGCA ATGGGGAAGT TGCCCCTGTT 
GGACCCACTT CATTTGAATT CTATGTGCCA GAAGACTGTC CTGTGACAAT CACACCTTCT 
GTTGGAACTG TGTTGCCTGG AAAGAAAAGC TTGATTCAAG TGTCCTTCAG ACCAACATTG 
TCAGATCAGC TAATCAGAGA AGAGGCAGCT CAGAGGCTTT GTGGTGCAGC TGCAACAGGG 
GCAGAAATAC AAGAAATAAA GAAAGATGAA AAGACAGAGA GAAAGAAATT AAGTGTTCCC 
CTTTCCAGAC GGCAGTCTTC CAGACAGGTT GTAAGGACTG GAAGCGTTAA ATGCCTGAGT 
TCTTCGTATA AATCTGAGCA ACCAGAATGC AAAGAGATAA AGCCTGACTC AGAAGCTTAT 
ATAGCAGCCC AGGCATTCCT GACAAGGAAT TTCAGAGGAA AGTTTGAAAA ATACACCATA 
CCATGCTTTG TTGCAAGTGG ACATGTTGAC GAAAAGAGAG GCTCGGAAAG TCTAAGCTTC 
AGCCCTTGTA ACACCTTGTA TTTGGAGCTG CATTGTCCAG CTGTAGCACC ATCTGTTGTG 
GTCACTTCAG ATAACGGAAA AAACACGGTT GATTTTGGTG ATGTGGCAGT AGGCCAAACA 
ATACTGAAGG AAACTACAAT ACAAAATATC TCCCCAGAAA ACCTGGAACT ACGATTCTCA 
GTTCTGAATC CCAGTGGTCC CTTCCTGTTG GTCAGACCAG TTAGGATGCT TGAGCCAGGT 
GAAATCAGAA CTCTCATCGT CTCTTTTTCT CCAAATAAGA ACAAATTGTT CCTTGAAATG 
CTGGACATCC GGACTGCAAA AAACAACTTG CCACTAAGGA TCACTGGTTA TGGGGTGGTG 
CCATCAACTG TTTGCTCTGT GGAAGTACTT GATATGGGCT ATGTCCTAAC CGGAGAGAAG 
GCAACGTCCA CATTCAAGGT ACAGAACACT TCCACGCTGA CCCTGCGATA CTCCATTCAT 
CTGGATTCAT TTTCATCCGC GAGGGACAAA GATCAGCAGA GGGTTCCATC TTTTCTTATG 
TCCTCTTTGC AAGGAGCAGA GACTGTTGAA ACAGAGAACT GTAATGGTGT AAGTGCATTC 
AGTGTCCTTC CGGCAGAAGG AGAAATTTGT GGTGGGGAAA GCCAGGACTT TATTGTTACT 
TTCAGTCCTG AGCGTGAAGG TCTATACTAT TCTGAGTGTC TCAAGGTTGT GCTCTTTGGC 
AAGAAAACTG CCCATGTGAT CCAGCTTAAG GGGGCAGCAA GAGATCATCC CATGTTTGTG 
GAAGGTGGGA TTCCACTAGA TGTGCCCATT GAGTCCTTGG CTGTAACGTT ACCTCCGTCA 
CCAAAAAAAG CACTAAAAGA AGAGCGACAA GAACCAGTGA AGTCCATTGT CCTGCTTCTG 
GAGCACGTTG AAGGAGAGAA TGCCCTACCG CCAGCAGTGG CAGAACTGAA AGTTGGAGCT 
ATACAGACAG CTCAGTTTGC ATCTAAGAAG AACGTGGAGT TCAGTTTTGA TAACCTGTCA 
CACCTCCAGA AGAAGGGATT TGCTATTGAA CCTGTAAAGG GAAAAGTTGA TCGTGGTCAT 
GTAAAATGCA TCAGTGTTTT TTGGGTGCCA CCTGCTGACT TCGATGCCAG TGCCCCCCTG 
GTTGAGACAG CCTTCCTGAA CTTAAAAGGT GACATTAAGG AATCTTACCG AATCCTCTTT 
GTGGCAACAG CTGTATCTGC TCCCACTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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C21H1ORF222 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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