ADAMTS20 cDNA ORF clone, Gallus gallus(chicken)

The following ADAMTS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142594 XM_025154135.1
Latest version!
Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa29177 XM_015282444.2
Latest version!
Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29178 XM_015282450.2
Latest version!
Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29179 XM_015282451.2
Latest version!
Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29180 XM_015282460.1
Latest version!
Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29181 XM_015282468.2
Latest version!
Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OGa29181 XM_015282468.1 Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29177 XM_015282444.1 Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29178 XM_015282450.1 Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa29179 XM_015282451.1 Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142594
    Clone ID Related Accession (Same CDS sequence) XM_025154135.1
    Accession Version XM_025154135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT ATCTATTGTT CAGACATGCA CTTAGACAAC CCCAAAGAAT ATTTGACGTT
    GTTAAAAGGA GAAGCAGACA ATTTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009903.1
    CDS89..5278
    Translation

    Target ORF information:

    RefSeq Version XM_025154135.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT ATCTATTGTT CAGACATGCA CTTAGACAAC CCCAAAGAAT ATTTGACGTT
    GTTAAAAGGA GAAGCAGACA ATTTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29177
    Clone ID Related Accession (Same CDS sequence) XM_015282444.2 , XM_015282444.1
    Accession Version XM_015282444.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT GTGAAATTAT TACCACCTGC AAAGATATCC AGATTAAAAA AGGGATGAGA
    AAAGACGGTG AATACCTACT CAACATTAAA GGAAGGATGA TAAAAATCTA TTGTTCAGAC
    ATGCACTTAG ACAACCCCAA AGAATATTTG ACGTTGTTAA AAGGAGAAGC AGACAATTTC
    TCTGAAGTGT ATGGACTCAG GTTACAGAAT CCATATGAAT GTCCTTTCAA TGGAAGCAGG
    AGGCAAGACT GTGCCTGTCG AAATGATTAC CTTGCAGCTG GCTTCACGGT TTTTAGCAAA
    ATAAGATTTG ATGTCGCCTC CATGCAAATT AGAACCACAG ATTTTCTTTT TGCTCAGACT
    ATACTTGGGA GAGCGGTTCC GTTTGCTACA GCTGGAGATT GCTACAGTGC TGCCAGATGT
    CCACAGGGAC AGTTCAGCAT TAATTTGGCT GGTACAGGTC TGAGATTATC CAGTACCGTT
    AGATGGATGG CTCAGGGCAA CTATGCAACC GCCGACATAC ACAAATCCCA CGATGGTACC
    AAAATATATG GAAGATGTGG AGGGTTTTGT GGGAAATGTG TTCCTAACAC AATTACTGGT
    CTTCAGCTTC AAATAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137930.1
    CDS89..5809
    Translation

    Target ORF information:

    RefSeq Version XM_015282444.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT GTGAAATTAT TACCACCTGC AAAGATATCC AGATTAAAAA AGGGATGAGA
    AAAGACGGTG AATACCTACT CAACATTAAA GGAAGGATGA TAAAAATCTA TTGTTCAGAC
    ATGCACTTAG ACAACCCCAA AGAATATTTG ACGTTGTTAA AAGGAGAAGC AGACAATTTC
    TCTGAAGTGT ATGGACTCAG GTTACAGAAT CCATATGAAT GTCCTTTCAA TGGAAGCAGG
    AGGCAAGACT GTGCCTGTCG AAATGATTAC CTTGCAGCTG GCTTCACGGT TTTTAGCAAA
    ATAAGATTTG ATGTCGCCTC CATGCAAATT AGAACCACAG ATTTTCTTTT TGCTCAGACT
    ATACTTGGGA GAGCGGTTCC GTTTGCTACA GCTGGAGATT GCTACAGTGC TGCCAGATGT
    CCACAGGGAC AGTTCAGCAT TAATTTGGCT GGTACAGGTC TGAGATTATC CAGTACCGTT
    AGATGGATGG CTCAGGGCAA CTATGCAACC GCCGACATAC ACAAATCCCA CGATGGTACC
    AAAATATATG GAAGATGTGG AGGGTTTTGT GGGAAATGTG TTCCTAACAC AATTACTGGT
    CTTCAGCTTC AAATAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29177
    Clone ID Related Accession (Same CDS sequence) XM_015282444.2 , XM_015282444.1
    Accession Version XM_015282444.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT GTGAAATTAT TACCACCTGC AAAGATATCC AGATTAAAAA AGGGATGAGA
    AAAGACGGTG AATACCTACT CAACATTAAA GGAAGGATGA TAAAAATCTA TTGTTCAGAC
    ATGCACTTAG ACAACCCCAA AGAATATTTG ACGTTGTTAA AAGGAGAAGC AGACAATTTC
    TCTGAAGTGT ATGGACTCAG GTTACAGAAT CCATATGAAT GTCCTTTCAA TGGAAGCAGG
    AGGCAAGACT GTGCCTGTCG AAATGATTAC CTTGCAGCTG GCTTCACGGT TTTTAGCAAA
    ATAAGATTTG ATGTCGCCTC CATGCAAATT AGAACCACAG ATTTTCTTTT TGCTCAGACT
    ATACTTGGGA GAGCGGTTCC GTTTGCTACA GCTGGAGATT GCTACAGTGC TGCCAGATGT
    CCACAGGGAC AGTTCAGCAT TAATTTGGCT GGTACAGGTC TGAGATTATC CAGTACCGTT
    AGATGGATGG CTCAGGGCAA CTATGCAACC GCCGACATAC ACAAATCCCA CGATGGTACC
    AAAATATATG GAAGATGTGG AGGGTTTTGT GGGAAATGTG TTCCTAACAC AATTACTGGT
    CTTCAGCTTC AAATAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137930.1
    CDS42..5762
    Translation

    Target ORF information:

    RefSeq Version XM_015282444.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT GTGAAATTAT TACCACCTGC AAAGATATCC AGATTAAAAA AGGGATGAGA
    AAAGACGGTG AATACCTACT CAACATTAAA GGAAGGATGA TAAAAATCTA TTGTTCAGAC
    ATGCACTTAG ACAACCCCAA AGAATATTTG ACGTTGTTAA AAGGAGAAGC AGACAATTTC
    TCTGAAGTGT ATGGACTCAG GTTACAGAAT CCATATGAAT GTCCTTTCAA TGGAAGCAGG
    AGGCAAGACT GTGCCTGTCG AAATGATTAC CTTGCAGCTG GCTTCACGGT TTTTAGCAAA
    ATAAGATTTG ATGTCGCCTC CATGCAAATT AGAACCACAG ATTTTCTTTT TGCTCAGACT
    ATACTTGGGA GAGCGGTTCC GTTTGCTACA GCTGGAGATT GCTACAGTGC TGCCAGATGT
    CCACAGGGAC AGTTCAGCAT TAATTTGGCT GGTACAGGTC TGAGATTATC CAGTACCGTT
    AGATGGATGG CTCAGGGCAA CTATGCAACC GCCGACATAC ACAAATCCCA CGATGGTACC
    AAAATATATG GAAGATGTGG AGGGTTTTGT GGGAAATGTG TTCCTAACAC AATTACTGGT
    CTTCAGCTTC AAATAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29178
    Clone ID Related Accession (Same CDS sequence) XM_015282450.2 , XM_015282450.1
    Accession Version XM_015282450.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    TACAATGTTG TAGTAAAGAT TCCCAAAGGA GCAACAAACA TTGATATTCG GCAGCACAGC
    TACTCAGGGA AACCAGAAGA TGACAACTAC CTTGCGTTAT CTGATATTCA TGGGAATTTT
    ATCTTGAATG GAAACTTTGT TGTAAGCATG TCAAAAAGAG AAATCAACAT ACATGGAGCA
    ATTTTTGAGT ACAATGGTTC AAACAATACG GTAGAACGAA TTAACAGCAC TGATAGGATT
    GAAGAGGAGT TAACCTTACA GGTTTTGTGT GTAGGGAATT TATACAACCC TGATGTGCGT
    TATTCATTCA ATATTCCAAT AGAAGACAGA AGTGATCTGT TTACGTGGGA TCCCTATGGA
    CCCTGGCAGG ACTGCAGCAG GATGTGCCAA GGAATTAGAA GAAGAAGAAT GACGTGCATA
    CGTAAAAGTG ATCATGTGGT AGTGTCTGAT CAAAGATGTG AACATATACC TCCTGTTCCA
    GATGTCTCTG AAGAGTGTAA TACAGAGTGT GAATTAAGGT GGCACAGTAT TGGCAAAAGT
    GACTGTACAT CAAAGTGTGG CCCAGGGTAT CGATCTATAG AAATCCACTG CATGAAGTAC
    TCTGTTTCAA AAGGACTCAG TGCTCTGGTG GACGATAGAT ACTGTGCTGA TCAGCAGAAA
    CCACCAACCA GAGAGGCCTG TCACGGCGAC TGTATGTTAA CAAGTTGGCA CTACGCAGAA
    TGGTCAGAGT GTTCCAGGAG CTGTGAAAGA GGTATCAGAA CCAGAGAAGC TTTCTGTTTG
    AACAACTTGG GTCGTCATCT TCCTGACAGG GAATGTCAGG AACTTCCAAG AGTTGTAACA
    GAGAGTTGCA ATGAATTCTT ATGTCCAAGC TGGTCTGCTA GTGAGTGGAG TGAGTGCCCT
    GTGACTTGTG GCAAAGGAAT GAAGCATCGA CAAGTATGGT GCCAACTTAA TGATGAACAA
    CTGAGAGATG ATTTCTGTAA TCTGAATGAC AGACCAGGAT CAGTAACGCC ATGTGAACTT
    CCTGAATGCG CATCTTGGCA AGTAGGGCCT TGGGGAGCAT GTACTGTAAC GTGTGGACGT
    GGATACCAGA TGCGTGCAGT CAGATGTGTT ACTGGGACTT ACAGAGTTAT TTTGGATGAT
    GACAGAGAAT GTAATGCTGC TACTCGCCCT AGAGATAACC AAGAATGTGA ATTGCCCTCT
    TGTCCAGAAA CTACAAAATT ATGGACGACA TCACTTCCTC TAATTCATGT AGGAAAGGTG
    ACCCAATGGC GATATGGATC ATGGACCCCA TGTTCAGCAT CCTGTGGGAA GGGTGATCGT
    GCTCGCTATG TGAGCTGTAG AGATGCTCAT GGAGGAATAG CAGATGAATC TTTCTGTGCT
    CATTTGCCAC GTCCAGCTGA AGTTTCAAGT TGCTTTAGCC CATGTGGAGA GTGGCAAGTT
    GGAAACTGGT CCCCTTGCAC AGTTACGTGT GATAGTGGAA TAATAACAAG ACAAGTTATC
    TGTGTCAACT ACCATCAACA AATCAATGAG AATTACTGCG ATCCTGAAGG CCGTCCCTCT
    AAAGAACAAG AATGTAACAT GCCACCATGT CCAGCAGTTT ATCATACTCC AAAAATACAT
    GAGCATCCAA GTCATTTTCC AGAAGCAGGT TACCCTCCAA TACATCATCC ACTAGACAAT
    ACAAACCAAT GGAATCATCC TTCTGTAGGA GGATATCAAT GGAGAACTGG ACCCTGGGGA
    GCATGCTCTA GTACTTGTGC AGGTGGATTT CACCGTCGAG TTGTAGTATG TCAAGACGAG
    GAAGGAAGAA GTGCTAGTTA CTGTGATGAG GCAAGTAAAC CTCCAGAGTC AAGACACTGT
    GATTCTGGAC CCTGCCCACG ATGGAACTAT GGAAACTGGG GAGAATGTAC TCAAACATGT
    GGAGATGGAA TAAAGACAAG ACTGGTGATT TGTCAGCTTC CTACAGGCCA AATGCTGGGT
    GATCAAAACT GTGAAATTCT AGACAAGCCA CCTAATATGG CACAATGTAA TGTGCATTCT
    TGCCCTGGTG ATGTTTCATG GCATCGGGGA CCATGGAAAT CGTGTTCTGT TTCCTGCGGG
    AAAGGTTTGA AGCATCGTGA TGTGCACTGT TTTAATAGCT TCCGGGCTAA AGTAGAGGAT
    GGAAACTGCA GACATTTAAA AAAGCCACGG ACATACAAAA TCTGTAGAGC CGGAAGATGT
    CCTACCTGGA AAACCAGCGG ATGGAAAGAA TGTTCTGTAT CCTGTGGTGT GGGGATTCAG
    CAAAGGGACA TCTACTGTCA GCTGAAGGGC CTGGGTAAAG TCAGTGAAGC AATGTGCAAC
    CACAATGCAC GGCCCGCTGG CCACAGGCCA TGTTGGCTGC CTGCCTGCAT GCGGTACCAA
    TGGCTGGCAG ATGGATGGGA GAATTGCTCG ACGTCATATA GAAGAAAGGA AATACACCAG
    AAGGTTAAGT GTGTGGATGA AAAGCAGATC CAAGTGGATG AAAGCTTCTG CGATCCTGCC
    ACAAAGCCTC CATCCGTCAA AAACTGCAGG AGAGCTCCCT CCAAATACAT TGTGCTCACA
    GGAGAACTGT CACAGTGTTC AGCCAGCTGT GGGTTGGATT ACCGGCAGAG GATCACATAC
    TGTGTTGGAA TCCATTATGT TCAAAACAAG CACGCTTACG GCCTTCGAAC TGTGGCATAT
    CGAGAATGCC CAGTAACACC CTCCCCATAC ATTTATAACT GTAATATCAA AGGATGCTCA
    CAAGCAGCTA CCTGGAGAGT GGGGAAGTGG ACCAAGTGCT CAGTGTCTTG TGGAGCAGGG
    GTAAAGGAGA GAACAGTAGC GTGTATGACT GAAAATGGCT TATCCAGTGA CTTGTGCCTG
    CCACATTTAA AACCAGATAA CAGGAGGATC TGCCATACAG AGGAATGTGA AATTATTACC
    ACCTGCAAAG ATATCCAGAT TAAAAAAGGG ATGAGAAAAG ACGGTGAATA CCTACTCAAC
    ATTAAAGGAA GGATGATAAA AATCTATTGT TCAGACATGC ACTTAGACAA CCCCAAAGAA
    TATTTGACGT TGTTAAAAGG AGAAGCAGAC AATTTCTCTG AAGTGTATGG ACTCAGGTTA
    CAGAATCCAT ATGAATGTCC TTTCAATGGA AGCAGGAGGC AAGACTGTGC CTGTCGAAAT
    GATTACCTTG CAGCTGGCTT CACGGTTTTT AGCAAAATAA GATTTGATGT CGCCTCCATG
    CAAATTAGAA CCACAGATTT TCTTTTTGCT CAGACTATAC TTGGGAGAGC GGTTCCGTTT
    GCTACAGCTG GAGATTGCTA CAGTGCTGCC AGATGTCCAC AGGGACAGTT CAGCATTAAT
    TTGGCTGGTA CAGGTCTGAG ATTATCCAGT ACCGTTAGAT GGATGGCTCA GGGCAACTAT
    GCAACCGCCG ACATACACAA ATCCCACGAT GGTACCAAAA TATATGGAAG ATGTGGAGGG
    TTTTGTGGGA AATGTGTTCC TAACACAATT ACTGGTCTTC AGCTTCAAAT AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137936.1
    CDS89..5785
    Translation

    Target ORF information:

    RefSeq Version XM_015282450.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282450.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    TACAATGTTG TAGTAAAGAT TCCCAAAGGA GCAACAAACA TTGATATTCG GCAGCACAGC
    TACTCAGGGA AACCAGAAGA TGACAACTAC CTTGCGTTAT CTGATATTCA TGGGAATTTT
    ATCTTGAATG GAAACTTTGT TGTAAGCATG TCAAAAAGAG AAATCAACAT ACATGGAGCA
    ATTTTTGAGT ACAATGGTTC AAACAATACG GTAGAACGAA TTAACAGCAC TGATAGGATT
    GAAGAGGAGT TAACCTTACA GGTTTTGTGT GTAGGGAATT TATACAACCC TGATGTGCGT
    TATTCATTCA ATATTCCAAT AGAAGACAGA AGTGATCTGT TTACGTGGGA TCCCTATGGA
    CCCTGGCAGG ACTGCAGCAG GATGTGCCAA GGAATTAGAA GAAGAAGAAT GACGTGCATA
    CGTAAAAGTG ATCATGTGGT AGTGTCTGAT CAAAGATGTG AACATATACC TCCTGTTCCA
    GATGTCTCTG AAGAGTGTAA TACAGAGTGT GAATTAAGGT GGCACAGTAT TGGCAAAAGT
    GACTGTACAT CAAAGTGTGG CCCAGGGTAT CGATCTATAG AAATCCACTG CATGAAGTAC
    TCTGTTTCAA AAGGACTCAG TGCTCTGGTG GACGATAGAT ACTGTGCTGA TCAGCAGAAA
    CCACCAACCA GAGAGGCCTG TCACGGCGAC TGTATGTTAA CAAGTTGGCA CTACGCAGAA
    TGGTCAGAGT GTTCCAGGAG CTGTGAAAGA GGTATCAGAA CCAGAGAAGC TTTCTGTTTG
    AACAACTTGG GTCGTCATCT TCCTGACAGG GAATGTCAGG AACTTCCAAG AGTTGTAACA
    GAGAGTTGCA ATGAATTCTT ATGTCCAAGC TGGTCTGCTA GTGAGTGGAG TGAGTGCCCT
    GTGACTTGTG GCAAAGGAAT GAAGCATCGA CAAGTATGGT GCCAACTTAA TGATGAACAA
    CTGAGAGATG ATTTCTGTAA TCTGAATGAC AGACCAGGAT CAGTAACGCC ATGTGAACTT
    CCTGAATGCG CATCTTGGCA AGTAGGGCCT TGGGGAGCAT GTACTGTAAC GTGTGGACGT
    GGATACCAGA TGCGTGCAGT CAGATGTGTT ACTGGGACTT ACAGAGTTAT TTTGGATGAT
    GACAGAGAAT GTAATGCTGC TACTCGCCCT AGAGATAACC AAGAATGTGA ATTGCCCTCT
    TGTCCAGAAA CTACAAAATT ATGGACGACA TCACTTCCTC TAATTCATGT AGGAAAGGTG
    ACCCAATGGC GATATGGATC ATGGACCCCA TGTTCAGCAT CCTGTGGGAA GGGTGATCGT
    GCTCGCTATG TGAGCTGTAG AGATGCTCAT GGAGGAATAG CAGATGAATC TTTCTGTGCT
    CATTTGCCAC GTCCAGCTGA AGTTTCAAGT TGCTTTAGCC CATGTGGAGA GTGGCAAGTT
    GGAAACTGGT CCCCTTGCAC AGTTACGTGT GATAGTGGAA TAATAACAAG ACAAGTTATC
    TGTGTCAACT ACCATCAACA AATCAATGAG AATTACTGCG ATCCTGAAGG CCGTCCCTCT
    AAAGAACAAG AATGTAACAT GCCACCATGT CCAGCAGTTT ATCATACTCC AAAAATACAT
    GAGCATCCAA GTCATTTTCC AGAAGCAGGT TACCCTCCAA TACATCATCC ACTAGACAAT
    ACAAACCAAT GGAATCATCC TTCTGTAGGA GGATATCAAT GGAGAACTGG ACCCTGGGGA
    GCATGCTCTA GTACTTGTGC AGGTGGATTT CACCGTCGAG TTGTAGTATG TCAAGACGAG
    GAAGGAAGAA GTGCTAGTTA CTGTGATGAG GCAAGTAAAC CTCCAGAGTC AAGACACTGT
    GATTCTGGAC CCTGCCCACG ATGGAACTAT GGAAACTGGG GAGAATGTAC TCAAACATGT
    GGAGATGGAA TAAAGACAAG ACTGGTGATT TGTCAGCTTC CTACAGGCCA AATGCTGGGT
    GATCAAAACT GTGAAATTCT AGACAAGCCA CCTAATATGG CACAATGTAA TGTGCATTCT
    TGCCCTGGTG ATGTTTCATG GCATCGGGGA CCATGGAAAT CGTGTTCTGT TTCCTGCGGG
    AAAGGTTTGA AGCATCGTGA TGTGCACTGT TTTAATAGCT TCCGGGCTAA AGTAGAGGAT
    GGAAACTGCA GACATTTAAA AAAGCCACGG ACATACAAAA TCTGTAGAGC CGGAAGATGT
    CCTACCTGGA AAACCAGCGG ATGGAAAGAA TGTTCTGTAT CCTGTGGTGT GGGGATTCAG
    CAAAGGGACA TCTACTGTCA GCTGAAGGGC CTGGGTAAAG TCAGTGAAGC AATGTGCAAC
    CACAATGCAC GGCCCGCTGG CCACAGGCCA TGTTGGCTGC CTGCCTGCAT GCGGTACCAA
    TGGCTGGCAG ATGGATGGGA GAATTGCTCG ACGTCATATA GAAGAAAGGA AATACACCAG
    AAGGTTAAGT GTGTGGATGA AAAGCAGATC CAAGTGGATG AAAGCTTCTG CGATCCTGCC
    ACAAAGCCTC CATCCGTCAA AAACTGCAGG AGAGCTCCCT CCAAATACAT TGTGCTCACA
    GGAGAACTGT CACAGTGTTC AGCCAGCTGT GGGTTGGATT ACCGGCAGAG GATCACATAC
    TGTGTTGGAA TCCATTATGT TCAAAACAAG CACGCTTACG GCCTTCGAAC TGTGGCATAT
    CGAGAATGCC CAGTAACACC CTCCCCATAC ATTTATAACT GTAATATCAA AGGATGCTCA
    CAAGCAGCTA CCTGGAGAGT GGGGAAGTGG ACCAAGTGCT CAGTGTCTTG TGGAGCAGGG
    GTAAAGGAGA GAACAGTAGC GTGTATGACT GAAAATGGCT TATCCAGTGA CTTGTGCCTG
    CCACATTTAA AACCAGATAA CAGGAGGATC TGCCATACAG AGGAATGTGA AATTATTACC
    ACCTGCAAAG ATATCCAGAT TAAAAAAGGG ATGAGAAAAG ACGGTGAATA CCTACTCAAC
    ATTAAAGGAA GGATGATAAA AATCTATTGT TCAGACATGC ACTTAGACAA CCCCAAAGAA
    TATTTGACGT TGTTAAAAGG AGAAGCAGAC AATTTCTCTG AAGTGTATGG ACTCAGGTTA
    CAGAATCCAT ATGAATGTCC TTTCAATGGA AGCAGGAGGC AAGACTGTGC CTGTCGAAAT
    GATTACCTTG CAGCTGGCTT CACGGTTTTT AGCAAAATAA GATTTGATGT CGCCTCCATG
    CAAATTAGAA CCACAGATTT TCTTTTTGCT CAGACTATAC TTGGGAGAGC GGTTCCGTTT
    GCTACAGCTG GAGATTGCTA CAGTGCTGCC AGATGTCCAC AGGGACAGTT CAGCATTAAT
    TTGGCTGGTA CAGGTCTGAG ATTATCCAGT ACCGTTAGAT GGATGGCTCA GGGCAACTAT
    GCAACCGCCG ACATACACAA ATCCCACGAT GGTACCAAAA TATATGGAAG ATGTGGAGGG
    TTTTGTGGGA AATGTGTTCC TAACACAATT ACTGGTCTTC AGCTTCAAAT AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29178
    Clone ID Related Accession (Same CDS sequence) XM_015282450.2 , XM_015282450.1
    Accession Version XM_015282450.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    TACAATGTTG TAGTAAAGAT TCCCAAAGGA GCAACAAACA TTGATATTCG GCAGCACAGC
    TACTCAGGGA AACCAGAAGA TGACAACTAC CTTGCGTTAT CTGATATTCA TGGGAATTTT
    ATCTTGAATG GAAACTTTGT TGTAAGCATG TCAAAAAGAG AAATCAACAT ACATGGAGCA
    ATTTTTGAGT ACAATGGTTC AAACAATACG GTAGAACGAA TTAACAGCAC TGATAGGATT
    GAAGAGGAGT TAACCTTACA GGTTTTGTGT GTAGGGAATT TATACAACCC TGATGTGCGT
    TATTCATTCA ATATTCCAAT AGAAGACAGA AGTGATCTGT TTACGTGGGA TCCCTATGGA
    CCCTGGCAGG ACTGCAGCAG GATGTGCCAA GGAATTAGAA GAAGAAGAAT GACGTGCATA
    CGTAAAAGTG ATCATGTGGT AGTGTCTGAT CAAAGATGTG AACATATACC TCCTGTTCCA
    GATGTCTCTG AAGAGTGTAA TACAGAGTGT GAATTAAGGT GGCACAGTAT TGGCAAAAGT
    GACTGTACAT CAAAGTGTGG CCCAGGGTAT CGATCTATAG AAATCCACTG CATGAAGTAC
    TCTGTTTCAA AAGGACTCAG TGCTCTGGTG GACGATAGAT ACTGTGCTGA TCAGCAGAAA
    CCACCAACCA GAGAGGCCTG TCACGGCGAC TGTATGTTAA CAAGTTGGCA CTACGCAGAA
    TGGTCAGAGT GTTCCAGGAG CTGTGAAAGA GGTATCAGAA CCAGAGAAGC TTTCTGTTTG
    AACAACTTGG GTCGTCATCT TCCTGACAGG GAATGTCAGG AACTTCCAAG AGTTGTAACA
    GAGAGTTGCA ATGAATTCTT ATGTCCAAGC TGGTCTGCTA GTGAGTGGAG TGAGTGCCCT
    GTGACTTGTG GCAAAGGAAT GAAGCATCGA CAAGTATGGT GCCAACTTAA TGATGAACAA
    CTGAGAGATG ATTTCTGTAA TCTGAATGAC AGACCAGGAT CAGTAACGCC ATGTGAACTT
    CCTGAATGCG CATCTTGGCA AGTAGGGCCT TGGGGAGCAT GTACTGTAAC GTGTGGACGT
    GGATACCAGA TGCGTGCAGT CAGATGTGTT ACTGGGACTT ACAGAGTTAT TTTGGATGAT
    GACAGAGAAT GTAATGCTGC TACTCGCCCT AGAGATAACC AAGAATGTGA ATTGCCCTCT
    TGTCCAGAAA CTACAAAATT ATGGACGACA TCACTTCCTC TAATTCATGT AGGAAAGGTG
    ACCCAATGGC GATATGGATC ATGGACCCCA TGTTCAGCAT CCTGTGGGAA GGGTGATCGT
    GCTCGCTATG TGAGCTGTAG AGATGCTCAT GGAGGAATAG CAGATGAATC TTTCTGTGCT
    CATTTGCCAC GTCCAGCTGA AGTTTCAAGT TGCTTTAGCC CATGTGGAGA GTGGCAAGTT
    GGAAACTGGT CCCCTTGCAC AGTTACGTGT GATAGTGGAA TAATAACAAG ACAAGTTATC
    TGTGTCAACT ACCATCAACA AATCAATGAG AATTACTGCG ATCCTGAAGG CCGTCCCTCT
    AAAGAACAAG AATGTAACAT GCCACCATGT CCAGCAGTTT ATCATACTCC AAAAATACAT
    GAGCATCCAA GTCATTTTCC AGAAGCAGGT TACCCTCCAA TACATCATCC ACTAGACAAT
    ACAAACCAAT GGAATCATCC TTCTGTAGGA GGATATCAAT GGAGAACTGG ACCCTGGGGA
    GCATGCTCTA GTACTTGTGC AGGTGGATTT CACCGTCGAG TTGTAGTATG TCAAGACGAG
    GAAGGAAGAA GTGCTAGTTA CTGTGATGAG GCAAGTAAAC CTCCAGAGTC AAGACACTGT
    GATTCTGGAC CCTGCCCACG ATGGAACTAT GGAAACTGGG GAGAATGTAC TCAAACATGT
    GGAGATGGAA TAAAGACAAG ACTGGTGATT TGTCAGCTTC CTACAGGCCA AATGCTGGGT
    GATCAAAACT GTGAAATTCT AGACAAGCCA CCTAATATGG CACAATGTAA TGTGCATTCT
    TGCCCTGGTG ATGTTTCATG GCATCGGGGA CCATGGAAAT CGTGTTCTGT TTCCTGCGGG
    AAAGGTTTGA AGCATCGTGA TGTGCACTGT TTTAATAGCT TCCGGGCTAA AGTAGAGGAT
    GGAAACTGCA GACATTTAAA AAAGCCACGG ACATACAAAA TCTGTAGAGC CGGAAGATGT
    CCTACCTGGA AAACCAGCGG ATGGAAAGAA TGTTCTGTAT CCTGTGGTGT GGGGATTCAG
    CAAAGGGACA TCTACTGTCA GCTGAAGGGC CTGGGTAAAG TCAGTGAAGC AATGTGCAAC
    CACAATGCAC GGCCCGCTGG CCACAGGCCA TGTTGGCTGC CTGCCTGCAT GCGGTACCAA
    TGGCTGGCAG ATGGATGGGA GAATTGCTCG ACGTCATATA GAAGAAAGGA AATACACCAG
    AAGGTTAAGT GTGTGGATGA AAAGCAGATC CAAGTGGATG AAAGCTTCTG CGATCCTGCC
    ACAAAGCCTC CATCCGTCAA AAACTGCAGG AGAGCTCCCT CCAAATACAT TGTGCTCACA
    GGAGAACTGT CACAGTGTTC AGCCAGCTGT GGGTTGGATT ACCGGCAGAG GATCACATAC
    TGTGTTGGAA TCCATTATGT TCAAAACAAG CACGCTTACG GCCTTCGAAC TGTGGCATAT
    CGAGAATGCC CAGTAACACC CTCCCCATAC ATTTATAACT GTAATATCAA AGGATGCTCA
    CAAGCAGCTA CCTGGAGAGT GGGGAAGTGG ACCAAGTGCT CAGTGTCTTG TGGAGCAGGG
    GTAAAGGAGA GAACAGTAGC GTGTATGACT GAAAATGGCT TATCCAGTGA CTTGTGCCTG
    CCACATTTAA AACCAGATAA CAGGAGGATC TGCCATACAG AGGAATGTGA AATTATTACC
    ACCTGCAAAG ATATCCAGAT TAAAAAAGGG ATGAGAAAAG ACGGTGAATA CCTACTCAAC
    ATTAAAGGAA GGATGATAAA AATCTATTGT TCAGACATGC ACTTAGACAA CCCCAAAGAA
    TATTTGACGT TGTTAAAAGG AGAAGCAGAC AATTTCTCTG AAGTGTATGG ACTCAGGTTA
    CAGAATCCAT ATGAATGTCC TTTCAATGGA AGCAGGAGGC AAGACTGTGC CTGTCGAAAT
    GATTACCTTG CAGCTGGCTT CACGGTTTTT AGCAAAATAA GATTTGATGT CGCCTCCATG
    CAAATTAGAA CCACAGATTT TCTTTTTGCT CAGACTATAC TTGGGAGAGC GGTTCCGTTT
    GCTACAGCTG GAGATTGCTA CAGTGCTGCC AGATGTCCAC AGGGACAGTT CAGCATTAAT
    TTGGCTGGTA CAGGTCTGAG ATTATCCAGT ACCGTTAGAT GGATGGCTCA GGGCAACTAT
    GCAACCGCCG ACATACACAA ATCCCACGAT GGTACCAAAA TATATGGAAG ATGTGGAGGG
    TTTTGTGGGA AATGTGTTCC TAACACAATT ACTGGTCTTC AGCTTCAAAT AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137936.1
    CDS42..5738
    Translation

    Target ORF information:

    RefSeq Version XM_015282450.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    TACAATGTTG TAGTAAAGAT TCCCAAAGGA GCAACAAACA TTGATATTCG GCAGCACAGC
    TACTCAGGGA AACCAGAAGA TGACAACTAC CTTGCGTTAT CTGATATTCA TGGGAATTTT
    ATCTTGAATG GAAACTTTGT TGTAAGCATG TCAAAAAGAG AAATCAACAT ACATGGAGCA
    ATTTTTGAGT ACAATGGTTC AAACAATACG GTAGAACGAA TTAACAGCAC TGATAGGATT
    GAAGAGGAGT TAACCTTACA GGTTTTGTGT GTAGGGAATT TATACAACCC TGATGTGCGT
    TATTCATTCA ATATTCCAAT AGAAGACAGA AGTGATCTGT TTACGTGGGA TCCCTATGGA
    CCCTGGCAGG ACTGCAGCAG GATGTGCCAA GGAATTAGAA GAAGAAGAAT GACGTGCATA
    CGTAAAAGTG ATCATGTGGT AGTGTCTGAT CAAAGATGTG AACATATACC TCCTGTTCCA
    GATGTCTCTG AAGAGTGTAA TACAGAGTGT GAATTAAGGT GGCACAGTAT TGGCAAAAGT
    GACTGTACAT CAAAGTGTGG CCCAGGGTAT CGATCTATAG AAATCCACTG CATGAAGTAC
    TCTGTTTCAA AAGGACTCAG TGCTCTGGTG GACGATAGAT ACTGTGCTGA TCAGCAGAAA
    CCACCAACCA GAGAGGCCTG TCACGGCGAC TGTATGTTAA CAAGTTGGCA CTACGCAGAA
    TGGTCAGAGT GTTCCAGGAG CTGTGAAAGA GGTATCAGAA CCAGAGAAGC TTTCTGTTTG
    AACAACTTGG GTCGTCATCT TCCTGACAGG GAATGTCAGG AACTTCCAAG AGTTGTAACA
    GAGAGTTGCA ATGAATTCTT ATGTCCAAGC TGGTCTGCTA GTGAGTGGAG TGAGTGCCCT
    GTGACTTGTG GCAAAGGAAT GAAGCATCGA CAAGTATGGT GCCAACTTAA TGATGAACAA
    CTGAGAGATG ATTTCTGTAA TCTGAATGAC AGACCAGGAT CAGTAACGCC ATGTGAACTT
    CCTGAATGCG CATCTTGGCA AGTAGGGCCT TGGGGAGCAT GTACTGTAAC GTGTGGACGT
    GGATACCAGA TGCGTGCAGT CAGATGTGTT ACTGGGACTT ACAGAGTTAT TTTGGATGAT
    GACAGAGAAT GTAATGCTGC TACTCGCCCT AGAGATAACC AAGAATGTGA ATTGCCCTCT
    TGTCCAGAAA CTACAAAATT ATGGACGACA TCACTTCCTC TAATTCATGT AGGAAAGGTG
    ACCCAATGGC GATATGGATC ATGGACCCCA TGTTCAGCAT CCTGTGGGAA GGGTGATCGT
    GCTCGCTATG TGAGCTGTAG AGATGCTCAT GGAGGAATAG CAGATGAATC TTTCTGTGCT
    CATTTGCCAC GTCCAGCTGA AGTTTCAAGT TGCTTTAGCC CATGTGGAGA GTGGCAAGTT
    GGAAACTGGT CCCCTTGCAC AGTTACGTGT GATAGTGGAA TAATAACAAG ACAAGTTATC
    TGTGTCAACT ACCATCAACA AATCAATGAG AATTACTGCG ATCCTGAAGG CCGTCCCTCT
    AAAGAACAAG AATGTAACAT GCCACCATGT CCAGCAGTTT ATCATACTCC AAAAATACAT
    GAGCATCCAA GTCATTTTCC AGAAGCAGGT TACCCTCCAA TACATCATCC ACTAGACAAT
    ACAAACCAAT GGAATCATCC TTCTGTAGGA GGATATCAAT GGAGAACTGG ACCCTGGGGA
    GCATGCTCTA GTACTTGTGC AGGTGGATTT CACCGTCGAG TTGTAGTATG TCAAGACGAG
    GAAGGAAGAA GTGCTAGTTA CTGTGATGAG GCAAGTAAAC CTCCAGAGTC AAGACACTGT
    GATTCTGGAC CCTGCCCACG ATGGAACTAT GGAAACTGGG GAGAATGTAC TCAAACATGT
    GGAGATGGAA TAAAGACAAG ACTGGTGATT TGTCAGCTTC CTACAGGCCA AATGCTGGGT
    GATCAAAACT GTGAAATTCT AGACAAGCCA CCTAATATGG CACAATGTAA TGTGCATTCT
    TGCCCTGGTG ATGTTTCATG GCATCGGGGA CCATGGAAAT CGTGTTCTGT TTCCTGCGGG
    AAAGGTTTGA AGCATCGTGA TGTGCACTGT TTTAATAGCT TCCGGGCTAA AGTAGAGGAT
    GGAAACTGCA GACATTTAAA AAAGCCACGG ACATACAAAA TCTGTAGAGC CGGAAGATGT
    CCTACCTGGA AAACCAGCGG ATGGAAAGAA TGTTCTGTAT CCTGTGGTGT GGGGATTCAG
    CAAAGGGACA TCTACTGTCA GCTGAAGGGC CTGGGTAAAG TCAGTGAAGC AATGTGCAAC
    CACAATGCAC GGCCCGCTGG CCACAGGCCA TGTTGGCTGC CTGCCTGCAT GCGGTACCAA
    TGGCTGGCAG ATGGATGGGA GAATTGCTCG ACGTCATATA GAAGAAAGGA AATACACCAG
    AAGGTTAAGT GTGTGGATGA AAAGCAGATC CAAGTGGATG AAAGCTTCTG CGATCCTGCC
    ACAAAGCCTC CATCCGTCAA AAACTGCAGG AGAGCTCCCT CCAAATACAT TGTGCTCACA
    GGAGAACTGT CACAGTGTTC AGCCAGCTGT GGGTTGGATT ACCGGCAGAG GATCACATAC
    TGTGTTGGAA TCCATTATGT TCAAAACAAG CACGCTTACG GCCTTCGAAC TGTGGCATAT
    CGAGAATGCC CAGTAACACC CTCCCCATAC ATTTATAACT GTAATATCAA AGGATGCTCA
    CAAGCAGCTA CCTGGAGAGT GGGGAAGTGG ACCAAGTGCT CAGTGTCTTG TGGAGCAGGG
    GTAAAGGAGA GAACAGTAGC GTGTATGACT GAAAATGGCT TATCCAGTGA CTTGTGCCTG
    CCACATTTAA AACCAGATAA CAGGAGGATC TGCCATACAG AGGAATGTGA AATTATTACC
    ACCTGCAAAG ATATCCAGAT TAAAAAAGGG ATGAGAAAAG ACGGTGAATA CCTACTCAAC
    ATTAAAGGAA GGATGATAAA AATCTATTGT TCAGACATGC ACTTAGACAA CCCCAAAGAA
    TATTTGACGT TGTTAAAAGG AGAAGCAGAC AATTTCTCTG AAGTGTATGG ACTCAGGTTA
    CAGAATCCAT ATGAATGTCC TTTCAATGGA AGCAGGAGGC AAGACTGTGC CTGTCGAAAT
    GATTACCTTG CAGCTGGCTT CACGGTTTTT AGCAAAATAA GATTTGATGT CGCCTCCATG
    CAAATTAGAA CCACAGATTT TCTTTTTGCT CAGACTATAC TTGGGAGAGC GGTTCCGTTT
    GCTACAGCTG GAGATTGCTA CAGTGCTGCC AGATGTCCAC AGGGACAGTT CAGCATTAAT
    TTGGCTGGTA CAGGTCTGAG ATTATCCAGT ACCGTTAGAT GGATGGCTCA GGGCAACTAT
    GCAACCGCCG ACATACACAA ATCCCACGAT GGTACCAAAA TATATGGAAG ATGTGGAGGG
    TTTTGTGGGA AATGTGTTCC TAACACAATT ACTGGTCTTC AGCTTCAAAT AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29179
    Clone ID Related Accession (Same CDS sequence) XM_015282451.2 , XM_015282451.1
    Accession Version XM_015282451.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GCTCAGTGTC TTGTGGAGCA
    GGGGTAAAGG AGAGAACAGT AGCGTGTATG ACTGAAAATG GCTTATCCAG TGACTTGTGC
    CTGCCACATT TAAAACCAGA TAACAGGAGG ATCTGCCATA CAGAGGAATG TGAAATTATT
    ACCACCTGCA AAGATATCCA GATTAAAAAA GGGATGAGAA AAGACGGTGA ATACCTACTC
    AACATTAAAG GAAGGATGAT AAAAATCTAT TGTTCAGACA TGCACTTAGA CAACCCCAAA
    GAATATTTGA CGTTGTTAAA AGGAGAAGCA GACAATTTCT CTGAAGTGTA TGGACTCAGG
    TTACAGAATC CATATGAATG TCCTTTCAAT GGAAGCAGGA GGCAAGACTG TGCCTGTCGA
    AATGATTACC TTGCAGCTGG CTTCACGGTT TTTAGCAAAA TAAGATTTGA TGTCGCCTCC
    ATGCAAATTA GAACCACAGA TTTTCTTTTT GCTCAGACTA TACTTGGGAG AGCGGTTCCG
    TTTGCTACAG CTGGAGATTG CTACAGTGCT GCCAGATGTC CACAGGGACA GTTCAGCATT
    AATTTGGCTG GTACAGGTCT GAGATTATCC AGTACCGTTA GATGGATGGC TCAGGGCAAC
    TATGCAACCG CCGACATACA CAAATCCCAC GATGGTACCA AAATATATGG AAGATGTGGA
    GGGTTTTGTG GGAAATGTGT TCCTAACACA ATTACTGGTC TTCAGCTTCA AATAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137937.1
    CDS89..5608
    Translation

    Target ORF information:

    RefSeq Version XM_015282451.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282451.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GCTCAGTGTC TTGTGGAGCA
    GGGGTAAAGG AGAGAACAGT AGCGTGTATG ACTGAAAATG GCTTATCCAG TGACTTGTGC
    CTGCCACATT TAAAACCAGA TAACAGGAGG ATCTGCCATA CAGAGGAATG TGAAATTATT
    ACCACCTGCA AAGATATCCA GATTAAAAAA GGGATGAGAA AAGACGGTGA ATACCTACTC
    AACATTAAAG GAAGGATGAT AAAAATCTAT TGTTCAGACA TGCACTTAGA CAACCCCAAA
    GAATATTTGA CGTTGTTAAA AGGAGAAGCA GACAATTTCT CTGAAGTGTA TGGACTCAGG
    TTACAGAATC CATATGAATG TCCTTTCAAT GGAAGCAGGA GGCAAGACTG TGCCTGTCGA
    AATGATTACC TTGCAGCTGG CTTCACGGTT TTTAGCAAAA TAAGATTTGA TGTCGCCTCC
    ATGCAAATTA GAACCACAGA TTTTCTTTTT GCTCAGACTA TACTTGGGAG AGCGGTTCCG
    TTTGCTACAG CTGGAGATTG CTACAGTGCT GCCAGATGTC CACAGGGACA GTTCAGCATT
    AATTTGGCTG GTACAGGTCT GAGATTATCC AGTACCGTTA GATGGATGGC TCAGGGCAAC
    TATGCAACCG CCGACATACA CAAATCCCAC GATGGTACCA AAATATATGG AAGATGTGGA
    GGGTTTTGTG GGAAATGTGT TCCTAACACA ATTACTGGTC TTCAGCTTCA AATAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29179
    Clone ID Related Accession (Same CDS sequence) XM_015282451.2 , XM_015282451.1
    Accession Version XM_015282451.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GCTCAGTGTC TTGTGGAGCA
    GGGGTAAAGG AGAGAACAGT AGCGTGTATG ACTGAAAATG GCTTATCCAG TGACTTGTGC
    CTGCCACATT TAAAACCAGA TAACAGGAGG ATCTGCCATA CAGAGGAATG TGAAATTATT
    ACCACCTGCA AAGATATCCA GATTAAAAAA GGGATGAGAA AAGACGGTGA ATACCTACTC
    AACATTAAAG GAAGGATGAT AAAAATCTAT TGTTCAGACA TGCACTTAGA CAACCCCAAA
    GAATATTTGA CGTTGTTAAA AGGAGAAGCA GACAATTTCT CTGAAGTGTA TGGACTCAGG
    TTACAGAATC CATATGAATG TCCTTTCAAT GGAAGCAGGA GGCAAGACTG TGCCTGTCGA
    AATGATTACC TTGCAGCTGG CTTCACGGTT TTTAGCAAAA TAAGATTTGA TGTCGCCTCC
    ATGCAAATTA GAACCACAGA TTTTCTTTTT GCTCAGACTA TACTTGGGAG AGCGGTTCCG
    TTTGCTACAG CTGGAGATTG CTACAGTGCT GCCAGATGTC CACAGGGACA GTTCAGCATT
    AATTTGGCTG GTACAGGTCT GAGATTATCC AGTACCGTTA GATGGATGGC TCAGGGCAAC
    TATGCAACCG CCGACATACA CAAATCCCAC GATGGTACCA AAATATATGG AAGATGTGGA
    GGGTTTTGTG GGAAATGTGT TCCTAACACA ATTACTGGTC TTCAGCTTCA AATAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137937.1
    CDS42..5561
    Translation

    Target ORF information:

    RefSeq Version XM_015282451.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GCTCAGTGTC TTGTGGAGCA
    GGGGTAAAGG AGAGAACAGT AGCGTGTATG ACTGAAAATG GCTTATCCAG TGACTTGTGC
    CTGCCACATT TAAAACCAGA TAACAGGAGG ATCTGCCATA CAGAGGAATG TGAAATTATT
    ACCACCTGCA AAGATATCCA GATTAAAAAA GGGATGAGAA AAGACGGTGA ATACCTACTC
    AACATTAAAG GAAGGATGAT AAAAATCTAT TGTTCAGACA TGCACTTAGA CAACCCCAAA
    GAATATTTGA CGTTGTTAAA AGGAGAAGCA GACAATTTCT CTGAAGTGTA TGGACTCAGG
    TTACAGAATC CATATGAATG TCCTTTCAAT GGAAGCAGGA GGCAAGACTG TGCCTGTCGA
    AATGATTACC TTGCAGCTGG CTTCACGGTT TTTAGCAAAA TAAGATTTGA TGTCGCCTCC
    ATGCAAATTA GAACCACAGA TTTTCTTTTT GCTCAGACTA TACTTGGGAG AGCGGTTCCG
    TTTGCTACAG CTGGAGATTG CTACAGTGCT GCCAGATGTC CACAGGGACA GTTCAGCATT
    AATTTGGCTG GTACAGGTCT GAGATTATCC AGTACCGTTA GATGGATGGC TCAGGGCAAC
    TATGCAACCG CCGACATACA CAAATCCCAC GATGGTACCA AAATATATGG AAGATGTGGA
    GGGTTTTGTG GGAAATGTGT TCCTAACACA ATTACTGGTC TTCAGCTTCA AATAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29180
    Clone ID Related Accession (Same CDS sequence) XM_015282460.1
    Accession Version XM_015282460.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT GTGAAATTAT TACCACCTGC AAAGATATCC AGATTAAAAA AGGGATGAGA
    AAAGACGGTG AATACCTACT CAACATTAAA GGAAGGATGA TAAAAATCTA TTGTTCAGAC
    ATGCACTTAG ACAACCCCAA AGAATATTTG ACGTTGTTAA AAGGAGAAGC AGACAATTTC
    TCTGAAGTGT ATGGACTCAG GTTACAGAAT CCATATGAAT GTCCTTTCAA TGGAAGCAGG
    AGGCAAGACT GTGCCTGTCG AAATGATTAC CTTGCAGCTG GCTTCACGGT TTTTAGCAAA
    ATAAGATTTG ATGTCGCCTC CATGCAAATT AGAAACTATA CTTGGGAGAG CGGTTCCGTT
    TGCTACAGCT GGAGATTGCT ACAGTGCTGC CAGATGTCCA CAGGGACAGT TCAGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137946.1
    CDS42..5561
    Translation

    Target ORF information:

    RefSeq Version XM_015282460.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCACAGTTAC GTGTGATAGT
    GGAATAATAA CAAGACAAGT TATCTGTGTC AACTACCATC AACAAATCAA TGAGAATTAC
    TGCGATCCTG AAGGCCGTCC CTCTAAAGAA CAAGAATGTA ACATGCCACC ATGTCCAGCA
    GTTTATCATA CTCCAAAAAT ACATGAGCAT CCAAGTCATT TTCCAGAAGC AGGTTACCCT
    CCAATACATC ATCCACTAGA CAATACAAAC CAATGGAATC ATCCTTCTGT AGGAGGATAT
    CAATGGAGAA CTGGACCCTG GGGAGCATGC TCTAGTACTT GTGCAGGTGG ATTTCACCGT
    CGAGTTGTAG TATGTCAAGA CGAGGAAGGA AGAAGTGCTA GTTACTGTGA TGAGGCAAGT
    AAACCTCCAG AGTCAAGACA CTGTGATTCT GGACCCTGCC CACGATGGAA CTATGGAAAC
    TGGGGAGAAT GTACTCAAAC ATGTGGAGAT GGAATAAAGA CAAGACTGGT GATTTGTCAG
    CTTCCTACAG GCCAAATGCT GGGTGATCAA AACTGTGAAA TTCTAGACAA GCCACCTAAT
    ATGGCACAAT GTAATGTGCA TTCTTGCCCT GGTGATGTTT CATGGCATCG GGGACCATGG
    AAATCGTGTT CTGTTTCCTG CGGGAAAGGT TTGAAGCATC GTGATGTGCA CTGTTTTAAT
    AGCTTCCGGG CTAAAGTAGA GGATGGAAAC TGCAGACATT TAAAAAAGCC ACGGACATAC
    AAAATCTGTA GAGCCGGAAG ATGTCCTACC TGGAAAACCA GCGGATGGAA AGAATGTTCT
    GTATCCTGTG GTGTGGGGAT TCAGCAAAGG GACATCTACT GTCAGCTGAA GGGCCTGGGT
    AAAGTCAGTG AAGCAATGTG CAACCACAAT GCACGGCCCG CTGGCCACAG GCCATGTTGG
    CTGCCTGCCT GCATGCGGTA CCAATGGCTG GCAGATGGAT GGGAGAATTG CTCGACGTCA
    TATAGAAGAA AGGAAATACA CCAGAAGGTT AAGTGTGTGG ATGAAAAGCA GATCCAAGTG
    GATGAAAGCT TCTGCGATCC TGCCACAAAG CCTCCATCCG TCAAAAACTG CAGGAGAGCT
    CCCTCCAAAT ACATTGTGCT CACAGGAGAA CTGTCACAGT GTTCAGCCAG CTGTGGGTTG
    GATTACCGGC AGAGGATCAC ATACTGTGTT GGAATCCATT ATGTTCAAAA CAAGCACGCT
    TACGGCCTTC GAACTGTGGC ATATCGAGAA TGCCCAGTAA CACCCTCCCC ATACATTTAT
    AACTGTAATA TCAAAGGATG CTCACAAGCA GCTACCTGGA GAGTGGGGAA GTGGACCAAG
    TGCTCAGTGT CTTGTGGAGC AGGGGTAAAG GAGAGAACAG TAGCGTGTAT GACTGAAAAT
    GGCTTATCCA GTGACTTGTG CCTGCCACAT TTAAAACCAG ATAACAGGAG GATCTGCCAT
    ACAGAGGAAT GTGAAATTAT TACCACCTGC AAAGATATCC AGATTAAAAA AGGGATGAGA
    AAAGACGGTG AATACCTACT CAACATTAAA GGAAGGATGA TAAAAATCTA TTGTTCAGAC
    ATGCACTTAG ACAACCCCAA AGAATATTTG ACGTTGTTAA AAGGAGAAGC AGACAATTTC
    TCTGAAGTGT ATGGACTCAG GTTACAGAAT CCATATGAAT GTCCTTTCAA TGGAAGCAGG
    AGGCAAGACT GTGCCTGTCG AAATGATTAC CTTGCAGCTG GCTTCACGGT TTTTAGCAAA
    ATAAGATTTG ATGTCGCCTC CATGCAAATT AGAAACTATA CTTGGGAGAG CGGTTCCGTT
    TGCTACAGCT GGAGATTGCT ACAGTGCTGC CAGATGTCCA CAGGGACAGT TCAGCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29181
    Clone ID Related Accession (Same CDS sequence) XM_015282468.2 , XM_015282468.1
    Accession Version XM_015282468.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015282468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCTCTAGTAC TTGTGCAGGT
    GGATTTCACC GTCGAGTTGT AGTATGTCAA GACGAGGAAG GAAGAAGTGC TAGTTACTGT
    GATGAGGCAA GTAAACCTCC AGAGTCAAGA CACTGTGATT CTGGACCCTG CCCACGATGG
    AACTATGGAA ACTGGGGAGA ATGTACTCAA ACATGTGGAG ATGGAATAAA GACAAGACTG
    GTGATTTGTC AGCTTCCTAC AGGCCAAATG CTGGGTGATC AAAACTGTGA AATTCTAGAC
    AAGCCACCTA ATATGGCACA ATGTAATGTG CATTCTTGCC CTGGTGATGT TTCATGGCAT
    CGGGGACCAT GGAAATCGTG TTCTGTTTCC TGCGGGAAAG GTTTGAAGCA TCGTGATGTG
    CACTGTTTTA ATAGCTTCCG GGCTAAAGTA GAGGATGGAA ACTGCAGACA TTTAAAAAAG
    CCACGGACAT ACAAAATCTG TAGAGCCGGA AGATGTCCTA CCTGGAAAAC CAGCGGATGG
    AAAGAATGTT CTGTATCCTG TGGTGTGGGG ATTCAGCAAA GGGACATCTA CTGTCAGCTG
    AAGGGCCTGG GTAAAGTCAG TGAAGCAATG TGCAACCACA ATGCACGGCC CGCTGGCCAC
    AGGCCATGTT GGCTGCCTGC CTGCATGCGG TACCAATGGC TGGCAGATGG ATGGGAGAAT
    TGCTCGACGT CATATAGAAG AAAGGAAATA CACCAGAAGG TTAAGTGTGT GGATGAAAAG
    CAGATCCAAG TGGATGAAAG CTTCTGCGAT CCTGCCACAA AGCCTCCATC CGTCAAAAAC
    TGCAGGAGAG CTCCCTCCAA ATACATTGTG CTCACAGGAG AACTGTCACA GTGTTCAGCC
    AGCTGTGGGT TGGATTACCG GCAGAGGATC ACATACTGTG TTGGAATCCA TTATGTTCAA
    AACAAGCACG CTTACGGCCT TCGAACTGTG GCATATCGAG AATGCCCAGT AACACCCTCC
    CCATACATTT ATAACTGTAA TATCAAAGGA TGCTCACAAG CAGCTACCTG GAGAGTGGGG
    AAGTGGACCA AGTGCTCAGT GTCTTGTGGA GCAGGGGTAA AGGAGAGAAC AGTAGCGTGT
    ATGACTGAAA ATGGCTTATC CAGTGACTTG TGCCTGCCAC ATTTAAAACC AGATAACAGG
    AGGATCTGCC ATACAGAGGA ATGTGAAATT ATTACCACCT GCAAAGATAT CCAGATTAAA
    AAAGGGATGA GAAAAGACGG TGAATACCTA CTCAACATTA AAGGAAGGAT GATAAAAATC
    TATTGTTCAG ACATGCACTT AGACAACCCC AAAGAATATT TGACGTTGTT AAAAGGAGAA
    GCAGACAATT TCTCTGAAGT GTATGGACTC AGGTTACAGA ATCCATATGA ATGTCCTTTC
    AATGGAAGCA GGAGGCAAGA CTGTGCCTGT CGAAATGATT ACCTTGCAGC TGGCTTCACG
    GTTTTTAGCA AAATAAGATT TGATGTCGCC TCCATGCAAA TTAGAACCAC AGATTTTCTT
    TTTGCTCAGA CTATACTTGG GAGAGCGGTT CCGTTTGCTA CAGCTGGAGA TTGCTACAGT
    GCTGCCAGAT GTCCACAGGG ACAGTTCAGC ATTAATTTGG CTGGTACAGG TCTGAGATTA
    TCCAGTACCG TTAGATGGAT GGCTCAGGGC AACTATGCAA CCGCCGACAT ACACAAATCC
    CACGATGGTA CCAAAATATA TGGAAGATGT GGAGGGTTTT GTGGGAAATG TGTTCCTAAC
    ACAATTACTG GTCTTCAGCT TCAAATAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137954.1
    CDS89..5521
    Translation

    Target ORF information:

    RefSeq Version XM_015282468.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282468.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCTCTAGTAC TTGTGCAGGT
    GGATTTCACC GTCGAGTTGT AGTATGTCAA GACGAGGAAG GAAGAAGTGC TAGTTACTGT
    GATGAGGCAA GTAAACCTCC AGAGTCAAGA CACTGTGATT CTGGACCCTG CCCACGATGG
    AACTATGGAA ACTGGGGAGA ATGTACTCAA ACATGTGGAG ATGGAATAAA GACAAGACTG
    GTGATTTGTC AGCTTCCTAC AGGCCAAATG CTGGGTGATC AAAACTGTGA AATTCTAGAC
    AAGCCACCTA ATATGGCACA ATGTAATGTG CATTCTTGCC CTGGTGATGT TTCATGGCAT
    CGGGGACCAT GGAAATCGTG TTCTGTTTCC TGCGGGAAAG GTTTGAAGCA TCGTGATGTG
    CACTGTTTTA ATAGCTTCCG GGCTAAAGTA GAGGATGGAA ACTGCAGACA TTTAAAAAAG
    CCACGGACAT ACAAAATCTG TAGAGCCGGA AGATGTCCTA CCTGGAAAAC CAGCGGATGG
    AAAGAATGTT CTGTATCCTG TGGTGTGGGG ATTCAGCAAA GGGACATCTA CTGTCAGCTG
    AAGGGCCTGG GTAAAGTCAG TGAAGCAATG TGCAACCACA ATGCACGGCC CGCTGGCCAC
    AGGCCATGTT GGCTGCCTGC CTGCATGCGG TACCAATGGC TGGCAGATGG ATGGGAGAAT
    TGCTCGACGT CATATAGAAG AAAGGAAATA CACCAGAAGG TTAAGTGTGT GGATGAAAAG
    CAGATCCAAG TGGATGAAAG CTTCTGCGAT CCTGCCACAA AGCCTCCATC CGTCAAAAAC
    TGCAGGAGAG CTCCCTCCAA ATACATTGTG CTCACAGGAG AACTGTCACA GTGTTCAGCC
    AGCTGTGGGT TGGATTACCG GCAGAGGATC ACATACTGTG TTGGAATCCA TTATGTTCAA
    AACAAGCACG CTTACGGCCT TCGAACTGTG GCATATCGAG AATGCCCAGT AACACCCTCC
    CCATACATTT ATAACTGTAA TATCAAAGGA TGCTCACAAG CAGCTACCTG GAGAGTGGGG
    AAGTGGACCA AGTGCTCAGT GTCTTGTGGA GCAGGGGTAA AGGAGAGAAC AGTAGCGTGT
    ATGACTGAAA ATGGCTTATC CAGTGACTTG TGCCTGCCAC ATTTAAAACC AGATAACAGG
    AGGATCTGCC ATACAGAGGA ATGTGAAATT ATTACCACCT GCAAAGATAT CCAGATTAAA
    AAAGGGATGA GAAAAGACGG TGAATACCTA CTCAACATTA AAGGAAGGAT GATAAAAATC
    TATTGTTCAG ACATGCACTT AGACAACCCC AAAGAATATT TGACGTTGTT AAAAGGAGAA
    GCAGACAATT TCTCTGAAGT GTATGGACTC AGGTTACAGA ATCCATATGA ATGTCCTTTC
    AATGGAAGCA GGAGGCAAGA CTGTGCCTGT CGAAATGATT ACCTTGCAGC TGGCTTCACG
    GTTTTTAGCA AAATAAGATT TGATGTCGCC TCCATGCAAA TTAGAACCAC AGATTTTCTT
    TTTGCTCAGA CTATACTTGG GAGAGCGGTT CCGTTTGCTA CAGCTGGAGA TTGCTACAGT
    GCTGCCAGAT GTCCACAGGG ACAGTTCAGC ATTAATTTGG CTGGTACAGG TCTGAGATTA
    TCCAGTACCG TTAGATGGAT GGCTCAGGGC AACTATGCAA CCGCCGACAT ACACAAATCC
    CACGATGGTA CCAAAATATA TGGAAGATGT GGAGGGTTTT GTGGGAAATG TGTTCCTAAC
    ACAATTACTG GTCTTCAGCT TCAAATAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29181
    Clone ID Related Accession (Same CDS sequence) XM_015282468.2 , XM_015282468.1
    Accession Version XM_015282468.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCTCTAGTAC TTGTGCAGGT
    GGATTTCACC GTCGAGTTGT AGTATGTCAA GACGAGGAAG GAAGAAGTGC TAGTTACTGT
    GATGAGGCAA GTAAACCTCC AGAGTCAAGA CACTGTGATT CTGGACCCTG CCCACGATGG
    AACTATGGAA ACTGGGGAGA ATGTACTCAA ACATGTGGAG ATGGAATAAA GACAAGACTG
    GTGATTTGTC AGCTTCCTAC AGGCCAAATG CTGGGTGATC AAAACTGTGA AATTCTAGAC
    AAGCCACCTA ATATGGCACA ATGTAATGTG CATTCTTGCC CTGGTGATGT TTCATGGCAT
    CGGGGACCAT GGAAATCGTG TTCTGTTTCC TGCGGGAAAG GTTTGAAGCA TCGTGATGTG
    CACTGTTTTA ATAGCTTCCG GGCTAAAGTA GAGGATGGAA ACTGCAGACA TTTAAAAAAG
    CCACGGACAT ACAAAATCTG TAGAGCCGGA AGATGTCCTA CCTGGAAAAC CAGCGGATGG
    AAAGAATGTT CTGTATCCTG TGGTGTGGGG ATTCAGCAAA GGGACATCTA CTGTCAGCTG
    AAGGGCCTGG GTAAAGTCAG TGAAGCAATG TGCAACCACA ATGCACGGCC CGCTGGCCAC
    AGGCCATGTT GGCTGCCTGC CTGCATGCGG TACCAATGGC TGGCAGATGG ATGGGAGAAT
    TGCTCGACGT CATATAGAAG AAAGGAAATA CACCAGAAGG TTAAGTGTGT GGATGAAAAG
    CAGATCCAAG TGGATGAAAG CTTCTGCGAT CCTGCCACAA AGCCTCCATC CGTCAAAAAC
    TGCAGGAGAG CTCCCTCCAA ATACATTGTG CTCACAGGAG AACTGTCACA GTGTTCAGCC
    AGCTGTGGGT TGGATTACCG GCAGAGGATC ACATACTGTG TTGGAATCCA TTATGTTCAA
    AACAAGCACG CTTACGGCCT TCGAACTGTG GCATATCGAG AATGCCCAGT AACACCCTCC
    CCATACATTT ATAACTGTAA TATCAAAGGA TGCTCACAAG CAGCTACCTG GAGAGTGGGG
    AAGTGGACCA AGTGCTCAGT GTCTTGTGGA GCAGGGGTAA AGGAGAGAAC AGTAGCGTGT
    ATGACTGAAA ATGGCTTATC CAGTGACTTG TGCCTGCCAC ATTTAAAACC AGATAACAGG
    AGGATCTGCC ATACAGAGGA ATGTGAAATT ATTACCACCT GCAAAGATAT CCAGATTAAA
    AAAGGGATGA GAAAAGACGG TGAATACCTA CTCAACATTA AAGGAAGGAT GATAAAAATC
    TATTGTTCAG ACATGCACTT AGACAACCCC AAAGAATATT TGACGTTGTT AAAAGGAGAA
    GCAGACAATT TCTCTGAAGT GTATGGACTC AGGTTACAGA ATCCATATGA ATGTCCTTTC
    AATGGAAGCA GGAGGCAAGA CTGTGCCTGT CGAAATGATT ACCTTGCAGC TGGCTTCACG
    GTTTTTAGCA AAATAAGATT TGATGTCGCC TCCATGCAAA TTAGAACCAC AGATTTTCTT
    TTTGCTCAGA CTATACTTGG GAGAGCGGTT CCGTTTGCTA CAGCTGGAGA TTGCTACAGT
    GCTGCCAGAT GTCCACAGGG ACAGTTCAGC ATTAATTTGG CTGGTACAGG TCTGAGATTA
    TCCAGTACCG TTAGATGGAT GGCTCAGGGC AACTATGCAA CCGCCGACAT ACACAAATCC
    CACGATGGTA CCAAAATATA TGGAAGATGT GGAGGGTTTT GTGGGAAATG TGTTCCTAAC
    ACAATTACTG GTCTTCAGCT TCAAATAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137954.1
    CDS42..5474
    Translation

    Target ORF information:

    RefSeq Version XM_015282468.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015282468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCGGGGGG CGAAGTGGCT GGCGGCTGTG CTGTGCCCCC TCGCCCTGCT CGTCGCCAGG 
    TTGTGGGCGT TTCGCCTCCA CCCGGGACAA GAGACCTTAG TGAAGCAGCT GTCCTCCTAT
    GAAATAGTCA CACCTGTCCG GGTGAATGAG TTCGGAGAAG TTTTTCCCCA CACGCGTCAC
    TTCAGCAGGA GGAAAAGGAG CTCGGAGCCC GCAGCACTCC GCACACACTA CCGGCTCAGC
    GCGTACGGGC AGCTCTTCCA GCTCAACCTG AGCGCCGACG CGGGCTTCAT CGCCGCGCAT
    TTCGCTGCGT TGCACGTCGG GGCCCCGCGG CAGCCGCCCT CCCCCGACCT GCGGCACTGC
    TTCTACCGCG GGCACGTCAA CGCGCAGCAG GCGCACGTGG CCGTCCTCAG CGTCTGCGGC
    GGCCTGATGG GCACCTTTAA AGCACATGAT GGTGAATATT TTTTAGAACC TCTGATGAAA
    ACGGATGGAG GTGAACATGA AGATGAGCAT AACAAACCAC ATCTTATATA CAGACATGAA
    GAAATTAATA GAAAGTATCA GAAATCCCAT AAACCTTGTG AAGTTTCAGA AAAAGAGGTG
    AAGAGCACTT TACTACATCC AAATGTCAGC AGTTTGAATG AAGAGAGAAA CATTTTACTG
    AAGTCTCCAA GTTTAGAGTA TGCTTCTAAA AATCTGTCTA TGGAGGATGC TGGCAATAAT
    CCCCATTCCC GGAAAAAACG TTTTCTTTCT TATCCAAGAT ATGTAGAAGT GATGGTTACA
    GCTGACACCA AAATGGTTCG TCATCATGGA CAGAATTTAC AGCACTACGT ATTAACACTG
    ATGTCAATAG TTGCAGCAAT CTATAAAGAT TCAAGTATTG GAAATCTTAT AAATATAGTT
    ATTGTAAAGT TAATTATAAT TCACAATGAA CAGGAAGGAC CAACTATCAC TTTTAATGCA
    GCTACCACTC TTCGTAATTT TTGTATATGG CAGCAAGCAC AAAACATTTT GGATGATGCT
    CACCCTTCTC ATCATGACAC TGCTGTTCTT ATCACTAGGG AAGATATCTG CAGAGCTAGA
    GAGAAGTGTG ATACTCTAGG TTTGGCAGAG CTAGGTACAA TGTGTGACCC ACTACGCAGC
    TGTTCTATAA GCGAAGAAAA TGGATTAAGT GCTGCTTTTA CTATAGCACA TGAGCTTGGA
    CATGTATTTA ACGTGCCACA TGATGACAGC TTTAAATGTA AAGAAGCTGG AATTAAACAT
    CAATACCATG TTATGGCTCC AACTTTAAAT TACCATACAA GTCCATGGAC CTGGTCAAAA
    TGCAGTCAAA AATACATCAC TGAATTTCTT GACACTGGTT ACGGTGAATG CCTCCTAGAT
    AAACCGAGTG GAAGAATTTA TGGTCTTTCT TCTCAGCTGC CTGGATTAAT GTATGATGTC
    AATAAGCAGT GCGAGCTTAT GTTTGGCCTT GGGTCGCAAG TGTGTCCCTA TCTGAAACAA
    TGCAAACGTT TATGGTGCAC GAGCACAGAG GGAGTTCACA AGGGATGTCG TACTCAGCAC
    ATGCCTTTGG CTGATGGAAC GGTTTGCGGT GTGGGAATGC ACTGCCGCCA TGGAATTTGT
    GTGAGCAGAG AATTGGAGAT GCGTCCAGTA GATGGAGAGT GGGGACCATG GGGACCTTAT
    AGCTCATGTT CAAGAACTTG TGGAGGTGGA ATCAAGAGCA CCACCAGGCT CTGTAACAGA
    CCAGAACCAA GAAATGGAGG AAAGTATTGT GTTGGCCGCA GGATGAAATT TCGCTCCTGT
    AATACTGATT CATGTCCAAA GGGCGAAAAA GATTTCCGGG AGCAACAGTG CTCTGAATTT
    GATGGCAAAC ATTTTAACAT CAATGGTCTT ACATCTGCTG TTCGCTGGCT TCCAAAATAC
    AGTGGTATTT CAATGAAAGA TCGCTGTAAA CTTTTTTGCC GAGTTTCTGG AACAACTTCC
    TACTATCAGC TGAAGGACAG AGTTGCTGAT GGTACTCCCT GTGGAGCAGA AACCAATGAC
    CTTTGTGTTC AAGGCTTATG CAGGCAAGCA GGCTGTGATC ATGTTTTGAA TTCCAAGGCA
    AGGAGAGACA AATGTGGAAT CTGTGGTGGT GATAATTCTT CATGTAAGAC ACTTGCAGGT
    ACTTTCAACC GTGCTCGTTA TGGTTACAAT GTTGTAGTAA AGATTCCCAA AGGAGCAACA
    AACATTGATA TTCGGCAGCA CAGCTACTCA GGGAAACCAG AAGATGACAA CTACCTTGCG
    TTATCTGATA TTCATGGGAA TTTTATCTTG AATGGAAACT TTGTTGTAAG CATGTCAAAA
    AGAGAAATCA ACATACATGG AGCAATTTTT GAGTACAATG GTTCAAACAA TACGGTAGAA
    CGAATTAACA GCACTGATAG GATTGAAGAG GAGTTAACCT TACAGGTTTT GTGTGTAGGG
    AATTTATACA ACCCTGATGT GCGTTATTCA TTCAATATTC CAATAGAAGA CAGAAGTGAT
    CTGTTTACGT GGGATCCCTA TGGACCCTGG CAGGACTGCA GCAGGATGTG CCAAGGAATT
    AGAAGAAGAA GAATGACGTG CATACGTAAA AGTGATCATG TGGTAGTGTC TGATCAAAGA
    TGTGAACATA TACCTCCTGT TCCAGATGTC TCTGAAGAGT GTAATACAGA GTGTGAATTA
    AGGTGGCACA GTATTGGCAA AAGTGACTGT ACATCAAAGT GTGGCCCAGG GTATCGATCT
    ATAGAAATCC ACTGCATGAA GTACTCTGTT TCAAAAGGAC TCAGTGCTCT GGTGGACGAT
    AGATACTGTG CTGATCAGCA GAAACCACCA ACCAGAGAGG CCTGTCACGG CGACTGTATG
    TTAACAAGTT GGCACTACGC AGAATGGTCA GAGTGTTCCA GGAGCTGTGA AAGAGGTATC
    AGAACCAGAG AAGCTTTCTG TTTGAACAAC TTGGGTCGTC ATCTTCCTGA CAGGGAATGT
    CAGGAACTTC CAAGAGTTGT AACAGAGAGT TGCAATGAAT TCTTATGTCC AAGCTGGTCT
    GCTAGTGAGT GGAGTGAGTG CCCTGTGACT TGTGGCAAAG GAATGAAGCA TCGACAAGTA
    TGGTGCCAAC TTAATGATGA ACAACTGAGA GATGATTTCT GTAATCTGAA TGACAGACCA
    GGATCAGTAA CGCCATGTGA ACTTCCTGAA TGCGCATCTT GGCAAGTAGG GCCTTGGGGA
    GCATGTACTG TAACGTGTGG ACGTGGATAC CAGATGCGTG CAGTCAGATG TGTTACTGGG
    ACTTACAGAG TTATTTTGGA TGATGACAGA GAATGTAATG CTGCTACTCG CCCTAGAGAT
    AACCAAGAAT GTGAATTGCC CTCTTGTCCA GAAACTACAA AATTATGGAC GACATCACTT
    CCTCTAATTC ATGTAGGAAA GGTGACCCAA TGGCGATATG GATCATGGAC CCCATGTTCA
    GCATCCTGTG GGAAGGGTGA TCGTGCTCGC TATGTGAGCT GTAGAGATGC TCATGGAGGA
    ATAGCAGATG AATCTTTCTG TGCTCATTTG CCACGTCCAG CTGAAGTTTC AAGTTGCTTT
    AGCCCATGTG GAGAGTGGCA AGTTGGAAAC TGGTCCCCTT GCTCTAGTAC TTGTGCAGGT
    GGATTTCACC GTCGAGTTGT AGTATGTCAA GACGAGGAAG GAAGAAGTGC TAGTTACTGT
    GATGAGGCAA GTAAACCTCC AGAGTCAAGA CACTGTGATT CTGGACCCTG CCCACGATGG
    AACTATGGAA ACTGGGGAGA ATGTACTCAA ACATGTGGAG ATGGAATAAA GACAAGACTG
    GTGATTTGTC AGCTTCCTAC AGGCCAAATG CTGGGTGATC AAAACTGTGA AATTCTAGAC
    AAGCCACCTA ATATGGCACA ATGTAATGTG CATTCTTGCC CTGGTGATGT TTCATGGCAT
    CGGGGACCAT GGAAATCGTG TTCTGTTTCC TGCGGGAAAG GTTTGAAGCA TCGTGATGTG
    CACTGTTTTA ATAGCTTCCG GGCTAAAGTA GAGGATGGAA ACTGCAGACA TTTAAAAAAG
    CCACGGACAT ACAAAATCTG TAGAGCCGGA AGATGTCCTA CCTGGAAAAC CAGCGGATGG
    AAAGAATGTT CTGTATCCTG TGGTGTGGGG ATTCAGCAAA GGGACATCTA CTGTCAGCTG
    AAGGGCCTGG GTAAAGTCAG TGAAGCAATG TGCAACCACA ATGCACGGCC CGCTGGCCAC
    AGGCCATGTT GGCTGCCTGC CTGCATGCGG TACCAATGGC TGGCAGATGG ATGGGAGAAT
    TGCTCGACGT CATATAGAAG AAAGGAAATA CACCAGAAGG TTAAGTGTGT GGATGAAAAG
    CAGATCCAAG TGGATGAAAG CTTCTGCGAT CCTGCCACAA AGCCTCCATC CGTCAAAAAC
    TGCAGGAGAG CTCCCTCCAA ATACATTGTG CTCACAGGAG AACTGTCACA GTGTTCAGCC
    AGCTGTGGGT TGGATTACCG GCAGAGGATC ACATACTGTG TTGGAATCCA TTATGTTCAA
    AACAAGCACG CTTACGGCCT TCGAACTGTG GCATATCGAG AATGCCCAGT AACACCCTCC
    CCATACATTT ATAACTGTAA TATCAAAGGA TGCTCACAAG CAGCTACCTG GAGAGTGGGG
    AAGTGGACCA AGTGCTCAGT GTCTTGTGGA GCAGGGGTAA AGGAGAGAAC AGTAGCGTGT
    ATGACTGAAA ATGGCTTATC CAGTGACTTG TGCCTGCCAC ATTTAAAACC AGATAACAGG
    AGGATCTGCC ATACAGAGGA ATGTGAAATT ATTACCACCT GCAAAGATAT CCAGATTAAA
    AAAGGGATGA GAAAAGACGG TGAATACCTA CTCAACATTA AAGGAAGGAT GATAAAAATC
    TATTGTTCAG ACATGCACTT AGACAACCCC AAAGAATATT TGACGTTGTT AAAAGGAGAA
    GCAGACAATT TCTCTGAAGT GTATGGACTC AGGTTACAGA ATCCATATGA ATGTCCTTTC
    AATGGAAGCA GGAGGCAAGA CTGTGCCTGT CGAAATGATT ACCTTGCAGC TGGCTTCACG
    GTTTTTAGCA AAATAAGATT TGATGTCGCC TCCATGCAAA TTAGAACCAC AGATTTTCTT
    TTTGCTCAGA CTATACTTGG GAGAGCGGTT CCGTTTGCTA CAGCTGGAGA TTGCTACAGT
    GCTGCCAGAT GTCCACAGGG ACAGTTCAGC ATTAATTTGG CTGGTACAGG TCTGAGATTA
    TCCAGTACCG TTAGATGGAT GGCTCAGGGC AACTATGCAA CCGCCGACAT ACACAAATCC
    CACGATGGTA CCAAAATATA TGGAAGATGT GGAGGGTTTT GTGGGAAATG TGTTCCTAAC
    ACAATTACTG GTCTTCAGCT TCAAATAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.