ANKLE2 cDNA ORF clone, Gallus gallus(chicken)

The following ANKLE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa144692 XM_004945490.3
Latest version!
Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa15431 XM_025155541.1
Latest version!
Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa15431 XM_025155540.1
Latest version!
Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa144691 XM_025155539.1
Latest version!
Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa15430 XM_004945490.2
Latest version!
Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa144692
    Clone ID Related Accession (Same CDS sequence) XM_004945490.3
    Accession Version XM_004945490.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ankyrin repeat and LEM domain-containing protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004945490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGCGCT GGGCCGGGGC GGCCGGCGGC GGCGGCTGGG GCTCGCTGTG GGACGGCGGC 
    TGGGACCGCT GGCCGGCCTG GGAGCTGCTG GTCGCCTGCG CGCTGATCGG CGCGGTGGGC
    TGGCTGCTGC GGCTGCTGGA CGGGCGGACG GGCGGGCGCG GGGCGCCGCC GGAGGGCCTC
    CGTTGCCCGA CCCCGCGCGG CGAGGCGGAG AGAGGGCGGC GCGGCGGGAG CCGCCCAGGT
    GAGATAACGA TGGATGCGAT CTTATCCCGC TTGAAGCAAC TCAGCCCTGA TGAGCTTAGA
    GAGGAACTTG TAAGAGCAGG GCTGAAGTGT GGGCCCGTCA CCTCAACTAC CAGGTTTATC
    TTTGAGAAGA AGTTGGCCCA GTTTTTACTG GAGCAGCAAG GAACGTTGGA AGAAGGGTCT
    GCATGGTCAG AGGAGGCTGC TGGGAGCGCC CCGCCTGATG GCACTCACAC AGCTTTGAAA
    ACAACTGCAG TGAATCACAG CCATCATGGA AGTGTGTCTG AAGAGGTCGA CTTTGGGTAC
    TGTGTCGGTC TGAACCCTCC AGAAGAAGAA GCTGTGGCAC ACACGAACTG TTCAGCTCCA
    GTGTGTGGGG CAGGATATGC TGGTTCACAA GGTGGTGCTC AGACACCATC CAGAGATCCT
    CCACTATACT ATGGTGTTTG CCCGGTCTAC GATGACATAC TGGCAAGAAA TGAGAGAATA
    CATGTTTATG AAGACAGGAA GGAAGCTCTC CAAGCTGTTA AGATGATTAA GGGTTCACGA
    TTTAAAGCGT TTTCAAACAG AGAAGATGCT GAGAAATTTG CTAAAGGAAT CTGTGATTAC
    TTTCCATCTC CAAGCAAGTC CTCTCTGTGT TTGTCACCTA TGAAAACAGG ATCACTTAAC
    AGAGATGGCT TATGCTCACT TGAGATGGAA ACTGCTAACA AGGAAAGGGC CAACAGCTAC
    AAAAGTCCAC GGACCCAGGA CCTCACTGCT AAGCTTCGGA AAGCTGTGGA GAAAGGAGAT
    ATATCAACAT TTTCAGAACT TATTTGGAGT AATCCTCGCT ATCTTATTGG ATCTGGAGAC
    AATCCAACAG TTGTACAGGA GGGGTGCAGG TACAATGTCA TGCACGTTGC TGCCAAGGAG
    AACCAGCCTG CTGTCTGCCA GCTGTTGTTG GACACTCTGG AAAATCCTGA ATTTATGCGG
    CTTATGTACC CAGATGATGA TAACACAATG CTGAGGAATC GCATCCGATA CATCGTTGAC
    CTTTACCTCA ATACACCAGA TAAAATGGGA TTTGATACTC CATTGCATTT TGCCTGCAAG
    TTTGGGAATT TGGATGTTGT TAATGTGCTT ACCTCACACC CAGCCATTGT AAAAAACCCC
    AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AGAGAAGCAA GAATAAATCT
    GCAGAATTGA AAGAAAAACT GAGAGAGTAT TTGAAAGGTC GCTACTATGT GCCACTTCTG
    AGAGCAGAAG ATAACTCCTC AGCTCCTGTC ATCGGTGCGC CGTGGTCACC GGATCAGACA
    GACGAGAGCC CGCAGGCAGC TCTGTCCAAA TACACGGGCA GCCCCAAGGA TCCGGTGCTG
    TCTATACGAG CTTTCGCAGG GCCCATGAGT CCCTCCAAGG CTGAAGAATT TCGCAGGCTG
    TGGAAGACTC CACCCCGAGA GAGAGCTGGA TTCTTTCACA ACGTCAGGAA ATCTGATCTG
    GAAAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AACTGGGGTT CCCGTGGGTT
    GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GCTGCGAAAA
    TTAGAAGAGT ACCTGAGCCA TCGGGAAATG AGCGAAAAGG CTCAGCAAGA AACAGGGGAA
    AATGAAACCT GCAACAGATA CAAAACGCCA CATCCTTCTG GCAAGAGTAA AAAGTGCTGC
    AATTCTATTT CTGTTGGAGC GTTTTTGGAT GAGGATGATG ATGACATGAG CTTAGAAGAA
    ATTAAAAACA GACAAAACGC AGCACGGAAT AACAGCCCAC CTGTTGTGCT GAAGGAACCC
    AGCATTGATG CTATTGGAGA CGCTGAGCGT GAGATCTTGT CAATGGAGCG TACCAGAAAC
    ATCATAGAAA CATCAGCTCA GCCCAGGCAC TACGAGAAGG GGGTTTCTTC CAGCAAAAAT
    GGATTTTGTA ATCCTGTGGC CAGTGAAAGG ATAATTGGTG ACAGAAAGCA TGTGCATGAC
    GGAGAAGATT GCCTTGTATC ACCTGTTTGC AATTTAATGT CACAGTTTGA AAGCTTAACA
    TTCCAACCAC AAGCGGGTGC AGGAGAATCA AATGCCATCA GTGAAAAAAA GGAGAGAATT
    CTGCCTGAAA GAACAAGCCA GGTTAGAATA CCCAAGGACT GGCTGGATAA GACCTGCTAC
    GCAGTTTCAC CACCAAGTTC TTCTGAGCCA AATGTTACAG GGAAACGTGG AGAGACCAAG
    TTAAGGACAG AACATAAAAT GGAGTCAGAA AGGGAAAGGT TATCCATGGA ATCTGGAGTC
    CAGACTAATG AGGCACAACA GTGTCAAGAA CCTCCTGCCC AGAAATCTCT TTTGAAGACT
    TCACCCACAA ATGACAACTC TAAGAAATTA TTTCTGCTTG GGGAGCGTCC ATCAAAGCTG
    GACAGTGACG TCTTAGCAGC TATCGAAGCA GTACAGATCG ATCCTCAGAA GTACCCCGCT
    ATTTACAGAT GGAAACACGA GGTGCAGTCC TACTCTTCAG CTGACAGACA AAGGGGGGAT
    GTGCAACTGG AAAAGCCTAC AACAAAGCCT CGTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945547.2
    CDS34..2892
    Translation

    Target ORF information:

    RefSeq Version XM_004945490.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945490.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGCGCT GGGCCGGGGC GGCCGGCGGC GGCGGCTGGG GCTCGCTGTG GGACGGCGGC 
    TGGGACCGCT GGCCGGCCTG GGAGCTGCTG GTCGCCTGCG CGCTGATCGG CGCGGTGGGC
    TGGCTGCTGC GGCTGCTGGA CGGGCGGACG GGCGGGCGCG GGGCGCCGCC GGAGGGCCTC
    CGTTGCCCGA CCCCGCGCGG CGAGGCGGAG AGAGGGCGGC GCGGCGGGAG CCGCCCAGGT
    GAGATAACGA TGGATGCGAT CTTATCCCGC TTGAAGCAAC TCAGCCCTGA TGAGCTTAGA
    GAGGAACTTG TAAGAGCAGG GCTGAAGTGT GGGCCCGTCA CCTCAACTAC CAGGTTTATC
    TTTGAGAAGA AGTTGGCCCA GTTTTTACTG GAGCAGCAAG GAACGTTGGA AGAAGGGTCT
    GCATGGTCAG AGGAGGCTGC TGGGAGCGCC CCGCCTGATG GCACTCACAC AGCTTTGAAA
    ACAACTGCAG TGAATCACAG CCATCATGGA AGTGTGTCTG AAGAGGTCGA CTTTGGGTAC
    TGTGTCGGTC TGAACCCTCC AGAAGAAGAA GCTGTGGCAC ACACGAACTG TTCAGCTCCA
    GTGTGTGGGG CAGGATATGC TGGTTCACAA GGTGGTGCTC AGACACCATC CAGAGATCCT
    CCACTATACT ATGGTGTTTG CCCGGTCTAC GATGACATAC TGGCAAGAAA TGAGAGAATA
    CATGTTTATG AAGACAGGAA GGAAGCTCTC CAAGCTGTTA AGATGATTAA GGGTTCACGA
    TTTAAAGCGT TTTCAAACAG AGAAGATGCT GAGAAATTTG CTAAAGGAAT CTGTGATTAC
    TTTCCATCTC CAAGCAAGTC CTCTCTGTGT TTGTCACCTA TGAAAACAGG ATCACTTAAC
    AGAGATGGCT TATGCTCACT TGAGATGGAA ACTGCTAACA AGGAAAGGGC CAACAGCTAC
    AAAAGTCCAC GGACCCAGGA CCTCACTGCT AAGCTTCGGA AAGCTGTGGA GAAAGGAGAT
    ATATCAACAT TTTCAGAACT TATTTGGAGT AATCCTCGCT ATCTTATTGG ATCTGGAGAC
    AATCCAACAG TTGTACAGGA GGGGTGCAGG TACAATGTCA TGCACGTTGC TGCCAAGGAG
    AACCAGCCTG CTGTCTGCCA GCTGTTGTTG GACACTCTGG AAAATCCTGA ATTTATGCGG
    CTTATGTACC CAGATGATGA TAACACAATG CTGAGGAATC GCATCCGATA CATCGTTGAC
    CTTTACCTCA ATACACCAGA TAAAATGGGA TTTGATACTC CATTGCATTT TGCCTGCAAG
    TTTGGGAATT TGGATGTTGT TAATGTGCTT ACCTCACACC CAGCCATTGT AAAAAACCCC
    AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AGAGAAGCAA GAATAAATCT
    GCAGAATTGA AAGAAAAACT GAGAGAGTAT TTGAAAGGTC GCTACTATGT GCCACTTCTG
    AGAGCAGAAG ATAACTCCTC AGCTCCTGTC ATCGGTGCGC CGTGGTCACC GGATCAGACA
    GACGAGAGCC CGCAGGCAGC TCTGTCCAAA TACACGGGCA GCCCCAAGGA TCCGGTGCTG
    TCTATACGAG CTTTCGCAGG GCCCATGAGT CCCTCCAAGG CTGAAGAATT TCGCAGGCTG
    TGGAAGACTC CACCCCGAGA GAGAGCTGGA TTCTTTCACA ACGTCAGGAA ATCTGATCTG
    GAAAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AACTGGGGTT CCCGTGGGTT
    GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GCTGCGAAAA
    TTAGAAGAGT ACCTGAGCCA TCGGGAAATG AGCGAAAAGG CTCAGCAAGA AACAGGGGAA
    AATGAAACCT GCAACAGATA CAAAACGCCA CATCCTTCTG GCAAGAGTAA AAAGTGCTGC
    AATTCTATTT CTGTTGGAGC GTTTTTGGAT GAGGATGATG ATGACATGAG CTTAGAAGAA
    ATTAAAAACA GACAAAACGC AGCACGGAAT AACAGCCCAC CTGTTGTGCT GAAGGAACCC
    AGCATTGATG CTATTGGAGA CGCTGAGCGT GAGATCTTGT CAATGGAGCG TACCAGAAAC
    ATCATAGAAA CATCAGCTCA GCCCAGGCAC TACGAGAAGG GGGTTTCTTC CAGCAAAAAT
    GGATTTTGTA ATCCTGTGGC CAGTGAAAGG ATAATTGGTG ACAGAAAGCA TGTGCATGAC
    GGAGAAGATT GCCTTGTATC ACCTGTTTGC AATTTAATGT CACAGTTTGA AAGCTTAACA
    TTCCAACCAC AAGCGGGTGC AGGAGAATCA AATGCCATCA GTGAAAAAAA GGAGAGAATT
    CTGCCTGAAA GAACAAGCCA GGTTAGAATA CCCAAGGACT GGCTGGATAA GACCTGCTAC
    GCAGTTTCAC CACCAAGTTC TTCTGAGCCA AATGTTACAG GGAAACGTGG AGAGACCAAG
    TTAAGGACAG AACATAAAAT GGAGTCAGAA AGGGAAAGGT TATCCATGGA ATCTGGAGTC
    CAGACTAATG AGGCACAACA GTGTCAAGAA CCTCCTGCCC AGAAATCTCT TTTGAAGACT
    TCACCCACAA ATGACAACTC TAAGAAATTA TTTCTGCTTG GGGAGCGTCC ATCAAAGCTG
    GACAGTGACG TCTTAGCAGC TATCGAAGCA GTACAGATCG ATCCTCAGAA GTACCCCGCT
    ATTTACAGAT GGAAACACGA GGTGCAGTCC TACTCTTCAG CTGACAGACA AAGGGGGGAT
    GTGCAACTGG AAAAGCCTAC AACAAAGCCT CGTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa15431
    Clone ID Related Accession (Same CDS sequence) XM_025155540.1 , XM_025155541.1
    Accession Version XM_025155540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ankyrin repeat and LEM domain-containing protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATGCGA TCTTATCCCG CTTGAAGCAA CTCAGCCCTG ATGAGCTTAG AGAGGAACTT 
    GTAAGAGCAG GGCTGAAGTG TGGGCCCGTC ACCTCAACTA CCAGGTTTAT CTTTGAGAAG
    AAGTTGGCCC AGTTTTTACT GGAGCAGCAA GGAACGTTGG AAGAAGGGTC TGCATGGTCA
    GAGGAGGCTG CTGGGAGCGC CCCGCCTGAT GGCACTCACA CAGCTTTGAA AACAACTGCA
    GTGAATCACA GCCATCATGG AAGTGTGTCT GAAGAGGTCG ACTTTGGGTA CTGTGTCGGT
    CTGAACCCTC CAGAAGAAGA AGCTGTGGCA CACACGAACT GTTCAGCTCC AGTGTGTGGG
    GCAGGATATG CTGGTTCACA AGGTGGTGCT CAGACACCAT CCAGAGATCC TCCACTATAC
    TATGGTGTTT GCCCGGTCTA CGATGACATA CTGGCAAGAA ATGAGAGAAT ACATGTTTAT
    GAAGACAGGA AGGAAGCTCT CCAAGCTGTT AAGATGATTA AGGGTTCACG ATTTAAAGCG
    TTTTCAAACA GAGAAGATGC TGAGAAATTT GCTAAAGGAA TCTGTGATTA CTTTCCATCT
    CCAAGCAAGT CCTCTCTGTG TTTGTCACCT ATGAAAACAG GATCACTTAA CAGAGATGGC
    TTATGCTCAC TTGAGATGGA AACTGCTAAC AAGGAAAGGG CCAACAGCTA CAAAAGTCCA
    CGGACCCAGG ACCTCACTGC TAAGCTTCGG AAAGCTGTGG AGAAAGGAGA TATATCAACA
    TTTTCAGAAC TTATTTGGAG TAATCCTCGC TATCTTATTG GATCTGGAGA CAATCCAACA
    GTTGTACAGG AGGGGTGCAG GTACAATGTC ATGCACGTTG CTGCCAAGGA GAACCAGCCT
    GCTGTCTGCC AGCTGTTGTT GGACACTCTG GAAAATCCTG AATTTATGCG GCTTATGTAC
    CCAGATGATG ATAACACAAT GCTGAGGAAT CGCATCCGAT ACATCGTTGA CCTTTACCTC
    AATACACCAG ATAAAATGGG ATTTGATACT CCATTGCATT TTGCCTGCAA GTTTGGGAAT
    TTGGATGTTG TTAATGTGCT TACCTCACAC CCAGCCATTG TAAAAAACCC CAGGAACAAA
    TATGATCAAA CTCCAGCAGA AGTAGTTTGT GAGAGAAGCA AGAATAAATC TGCAGAATTG
    AAAGAAAAAC TGAGAGAGTA TTTGAAAGGT CGCTACTATG TGCCACTTCT GAGAGCAGAA
    GATAACTCCT CAGCTCCTGT CATCGGTGCG CCGTGGTCAC CGGATCAGAC AGACGAGAGC
    CCGCAGGCAG CTCTGTCCAA ATACACGGGC AGCCCCAAGG ATCCGGTGCT GTCTATACGA
    GCTTTCGCAG GGCCCATGAG TCCCTCCAAG GCTGAAGAAT TTCGCAGGCT GTGGAAGACT
    CCACCCCGAG AGAGAGCTGG ATTCTTTCAC AACGTCAGGA AATCTGATCT GGAAAGAGGT
    GTTGAAAGAG TTGGAAGGGA GTTAGCTCAT GAACTGGGGT TCCCGTGGGT TGAATACTGG
    GAATTTCTGG GCTGTTTTGT TGATCTGTCT TCCCAGGAGG GGCTGCGAAA ATTAGAAGAG
    TACCTGAGCC ATCGGGAAAT GAGCGAAAAG GCTCAGCAAG AAACAGGGGA AAATGAAACC
    TGCAACAGAT ACAAAACGCC ACATCCTTCT GGCAAGAGTA AAAAGTGCTG CAATTCTATT
    TCTGTTGGAG CGTTTTTGGA TGAGGATGAT GATGACATGA GCTTAGAAGA AATTAAAAAC
    AGACAAAACG CAGCACGGAA TAACAGCCCA CCTGTTGTGC TGAAGGAACC CAGCATTGAT
    GCTATTGGAG ACGCTGAGCG TGAGATCTTG TCAATGGAGC GTACCAGAAA CATCATAGAA
    ACATCAGCTC AGCCCAGGCA CTACGAGAAG GGGGTTTCTT CCAGCAAAAA TGGATTTTGT
    AATCCTGTGG CCAGTGAAAG GATAATTGGT GACAGAAAGC ATGTGCATGA CGGAGAAGAT
    TGCCTTGTAT CACCTGTTTG CAATTTAATG TCACAGTTTG AAAGCTTAAC ATTCCAACCA
    CAAGCGGGTG CAGGAGAATC AAATGCCATC AGTGAAAAAA AGGAGAGAAT TCTGCCTGAA
    AGAACAAGCC AGGTTAGAAT ACCCAAGGAC TGGCTGGATA AGACCTGCTA CGCAGTTTCA
    CCACCAAGTT CTTCTGAGCC AAATGTTACA GGGAAACGTG GAGAGACCAA GTTAAGGACA
    GAACATAAAA TGGAGTCAGA AAGGGAAAGG TTATCCATGG AATCTGGAGT CCAGACTAAT
    GAGGCACAAC AGTGTCAAGA ACCTCCTGCC CAGAAATCTC TTTTGAAGAC TTCACCCACA
    AATGACAACT CTAAGAAATT ATTTCTGCTT GGGGAGCGTC CATCAAAGCT GGACAGTGAC
    GTCTTAGCAG CTATCGAAGC AGTACAGATC GATCCTCAGA AGTACCCCGC TATTTACAGA
    TGGAAACACG AGGTGCAGTC CTACTCTTCA GCTGACAGAC AAAGTTGGCC AAGTCCAGCA
    TTGAAGGGAA GATTCAGGTC CCAAAGCACA GCTGCTGGCC CACCAAATGC TTCAGTGTAT
    TCTAGTCCAG GAAGAAACAG TCCCATGATG GGAAGTCCAG GGAAACATGG CAATGCTGCC
    TCGTTTTCTC CAGACCCTGG GAGCCCTGGC CGCTACAGTC CTGCCTGTGT CAACCACTCT
    ATATTAAAAC CACGTTATTT TTCAGAGCCT CCTACCCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025011308.1
    CDS398..3199
    Translation

    Target ORF information:

    RefSeq Version XM_025155540.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025155540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATGCGA TCTTATCCCG CTTGAAGCAA CTCAGCCCTG ATGAGCTTAG AGAGGAACTT 
    GTAAGAGCAG GGCTGAAGTG TGGGCCCGTC ACCTCAACTA CCAGGTTTAT CTTTGAGAAG
    AAGTTGGCCC AGTTTTTACT GGAGCAGCAA GGAACGTTGG AAGAAGGGTC TGCATGGTCA
    GAGGAGGCTG CTGGGAGCGC CCCGCCTGAT GGCACTCACA CAGCTTTGAA AACAACTGCA
    GTGAATCACA GCCATCATGG AAGTGTGTCT GAAGAGGTCG ACTTTGGGTA CTGTGTCGGT
    CTGAACCCTC CAGAAGAAGA AGCTGTGGCA CACACGAACT GTTCAGCTCC AGTGTGTGGG
    GCAGGATATG CTGGTTCACA AGGTGGTGCT CAGACACCAT CCAGAGATCC TCCACTATAC
    TATGGTGTTT GCCCGGTCTA CGATGACATA CTGGCAAGAA ATGAGAGAAT ACATGTTTAT
    GAAGACAGGA AGGAAGCTCT CCAAGCTGTT AAGATGATTA AGGGTTCACG ATTTAAAGCG
    TTTTCAAACA GAGAAGATGC TGAGAAATTT GCTAAAGGAA TCTGTGATTA CTTTCCATCT
    CCAAGCAAGT CCTCTCTGTG TTTGTCACCT ATGAAAACAG GATCACTTAA CAGAGATGGC
    TTATGCTCAC TTGAGATGGA AACTGCTAAC AAGGAAAGGG CCAACAGCTA CAAAAGTCCA
    CGGACCCAGG ACCTCACTGC TAAGCTTCGG AAAGCTGTGG AGAAAGGAGA TATATCAACA
    TTTTCAGAAC TTATTTGGAG TAATCCTCGC TATCTTATTG GATCTGGAGA CAATCCAACA
    GTTGTACAGG AGGGGTGCAG GTACAATGTC ATGCACGTTG CTGCCAAGGA GAACCAGCCT
    GCTGTCTGCC AGCTGTTGTT GGACACTCTG GAAAATCCTG AATTTATGCG GCTTATGTAC
    CCAGATGATG ATAACACAAT GCTGAGGAAT CGCATCCGAT ACATCGTTGA CCTTTACCTC
    AATACACCAG ATAAAATGGG ATTTGATACT CCATTGCATT TTGCCTGCAA GTTTGGGAAT
    TTGGATGTTG TTAATGTGCT TACCTCACAC CCAGCCATTG TAAAAAACCC CAGGAACAAA
    TATGATCAAA CTCCAGCAGA AGTAGTTTGT GAGAGAAGCA AGAATAAATC TGCAGAATTG
    AAAGAAAAAC TGAGAGAGTA TTTGAAAGGT CGCTACTATG TGCCACTTCT GAGAGCAGAA
    GATAACTCCT CAGCTCCTGT CATCGGTGCG CCGTGGTCAC CGGATCAGAC AGACGAGAGC
    CCGCAGGCAG CTCTGTCCAA ATACACGGGC AGCCCCAAGG ATCCGGTGCT GTCTATACGA
    GCTTTCGCAG GGCCCATGAG TCCCTCCAAG GCTGAAGAAT TTCGCAGGCT GTGGAAGACT
    CCACCCCGAG AGAGAGCTGG ATTCTTTCAC AACGTCAGGA AATCTGATCT GGAAAGAGGT
    GTTGAAAGAG TTGGAAGGGA GTTAGCTCAT GAACTGGGGT TCCCGTGGGT TGAATACTGG
    GAATTTCTGG GCTGTTTTGT TGATCTGTCT TCCCAGGAGG GGCTGCGAAA ATTAGAAGAG
    TACCTGAGCC ATCGGGAAAT GAGCGAAAAG GCTCAGCAAG AAACAGGGGA AAATGAAACC
    TGCAACAGAT ACAAAACGCC ACATCCTTCT GGCAAGAGTA AAAAGTGCTG CAATTCTATT
    TCTGTTGGAG CGTTTTTGGA TGAGGATGAT GATGACATGA GCTTAGAAGA AATTAAAAAC
    AGACAAAACG CAGCACGGAA TAACAGCCCA CCTGTTGTGC TGAAGGAACC CAGCATTGAT
    GCTATTGGAG ACGCTGAGCG TGAGATCTTG TCAATGGAGC GTACCAGAAA CATCATAGAA
    ACATCAGCTC AGCCCAGGCA CTACGAGAAG GGGGTTTCTT CCAGCAAAAA TGGATTTTGT
    AATCCTGTGG CCAGTGAAAG GATAATTGGT GACAGAAAGC ATGTGCATGA CGGAGAAGAT
    TGCCTTGTAT CACCTGTTTG CAATTTAATG TCACAGTTTG AAAGCTTAAC ATTCCAACCA
    CAAGCGGGTG CAGGAGAATC AAATGCCATC AGTGAAAAAA AGGAGAGAAT TCTGCCTGAA
    AGAACAAGCC AGGTTAGAAT ACCCAAGGAC TGGCTGGATA AGACCTGCTA CGCAGTTTCA
    CCACCAAGTT CTTCTGAGCC AAATGTTACA GGGAAACGTG GAGAGACCAA GTTAAGGACA
    GAACATAAAA TGGAGTCAGA AAGGGAAAGG TTATCCATGG AATCTGGAGT CCAGACTAAT
    GAGGCACAAC AGTGTCAAGA ACCTCCTGCC CAGAAATCTC TTTTGAAGAC TTCACCCACA
    AATGACAACT CTAAGAAATT ATTTCTGCTT GGGGAGCGTC CATCAAAGCT GGACAGTGAC
    GTCTTAGCAG CTATCGAAGC AGTACAGATC GATCCTCAGA AGTACCCCGC TATTTACAGA
    TGGAAACACG AGGTGCAGTC CTACTCTTCA GCTGACAGAC AAAGTTGGCC AAGTCCAGCA
    TTGAAGGGAA GATTCAGGTC CCAAAGCACA GCTGCTGGCC CACCAAATGC TTCAGTGTAT
    TCTAGTCCAG GAAGAAACAG TCCCATGATG GGAAGTCCAG GGAAACATGG CAATGCTGCC
    TCGTTTTCTC CAGACCCTGG GAGCCCTGGC CGCTACAGTC CTGCCTGTGT CAACCACTCT
    ATATTAAAAC CACGTTATTT TTCAGAGCCT CCTACCCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa15431
    Clone ID Related Accession (Same CDS sequence) XM_025155540.1 , XM_025155541.1
    Accession Version XM_025155541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ankyrin repeat and LEM domain-containing protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATGCGA TCTTATCCCG CTTGAAGCAA CTCAGCCCTG ATGAGCTTAG AGAGGAACTT 
    GTAAGAGCAG GGCTGAAGTG TGGGCCCGTC ACCTCAACTA CCAGGTTTAT CTTTGAGAAG
    AAGTTGGCCC AGTTTTTACT GGAGCAGCAA GGAACGTTGG AAGAAGGGTC TGCATGGTCA
    GAGGAGGCTG CTGGGAGCGC CCCGCCTGAT GGCACTCACA CAGCTTTGAA AACAACTGCA
    GTGAATCACA GCCATCATGG AAGTGTGTCT GAAGAGGTCG ACTTTGGGTA CTGTGTCGGT
    CTGAACCCTC CAGAAGAAGA AGCTGTGGCA CACACGAACT GTTCAGCTCC AGTGTGTGGG
    GCAGGATATG CTGGTTCACA AGGTGGTGCT CAGACACCAT CCAGAGATCC TCCACTATAC
    TATGGTGTTT GCCCGGTCTA CGATGACATA CTGGCAAGAA ATGAGAGAAT ACATGTTTAT
    GAAGACAGGA AGGAAGCTCT CCAAGCTGTT AAGATGATTA AGGGTTCACG ATTTAAAGCG
    TTTTCAAACA GAGAAGATGC TGAGAAATTT GCTAAAGGAA TCTGTGATTA CTTTCCATCT
    CCAAGCAAGT CCTCTCTGTG TTTGTCACCT ATGAAAACAG GATCACTTAA CAGAGATGGC
    TTATGCTCAC TTGAGATGGA AACTGCTAAC AAGGAAAGGG CCAACAGCTA CAAAAGTCCA
    CGGACCCAGG ACCTCACTGC TAAGCTTCGG AAAGCTGTGG AGAAAGGAGA TATATCAACA
    TTTTCAGAAC TTATTTGGAG TAATCCTCGC TATCTTATTG GATCTGGAGA CAATCCAACA
    GTTGTACAGG AGGGGTGCAG GTACAATGTC ATGCACGTTG CTGCCAAGGA GAACCAGCCT
    GCTGTCTGCC AGCTGTTGTT GGACACTCTG GAAAATCCTG AATTTATGCG GCTTATGTAC
    CCAGATGATG ATAACACAAT GCTGAGGAAT CGCATCCGAT ACATCGTTGA CCTTTACCTC
    AATACACCAG ATAAAATGGG ATTTGATACT CCATTGCATT TTGCCTGCAA GTTTGGGAAT
    TTGGATGTTG TTAATGTGCT TACCTCACAC CCAGCCATTG TAAAAAACCC CAGGAACAAA
    TATGATCAAA CTCCAGCAGA AGTAGTTTGT GAGAGAAGCA AGAATAAATC TGCAGAATTG
    AAAGAAAAAC TGAGAGAGTA TTTGAAAGGT CGCTACTATG TGCCACTTCT GAGAGCAGAA
    GATAACTCCT CAGCTCCTGT CATCGGTGCG CCGTGGTCAC CGGATCAGAC AGACGAGAGC
    CCGCAGGCAG CTCTGTCCAA ATACACGGGC AGCCCCAAGG ATCCGGTGCT GTCTATACGA
    GCTTTCGCAG GGCCCATGAG TCCCTCCAAG GCTGAAGAAT TTCGCAGGCT GTGGAAGACT
    CCACCCCGAG AGAGAGCTGG ATTCTTTCAC AACGTCAGGA AATCTGATCT GGAAAGAGGT
    GTTGAAAGAG TTGGAAGGGA GTTAGCTCAT GAACTGGGGT TCCCGTGGGT TGAATACTGG
    GAATTTCTGG GCTGTTTTGT TGATCTGTCT TCCCAGGAGG GGCTGCGAAA ATTAGAAGAG
    TACCTGAGCC ATCGGGAAAT GAGCGAAAAG GCTCAGCAAG AAACAGGGGA AAATGAAACC
    TGCAACAGAT ACAAAACGCC ACATCCTTCT GGCAAGAGTA AAAAGTGCTG CAATTCTATT
    TCTGTTGGAG CGTTTTTGGA TGAGGATGAT GATGACATGA GCTTAGAAGA AATTAAAAAC
    AGACAAAACG CAGCACGGAA TAACAGCCCA CCTGTTGTGC TGAAGGAACC CAGCATTGAT
    GCTATTGGAG ACGCTGAGCG TGAGATCTTG TCAATGGAGC GTACCAGAAA CATCATAGAA
    ACATCAGCTC AGCCCAGGCA CTACGAGAAG GGGGTTTCTT CCAGCAAAAA TGGATTTTGT
    AATCCTGTGG CCAGTGAAAG GATAATTGGT GACAGAAAGC ATGTGCATGA CGGAGAAGAT
    TGCCTTGTAT CACCTGTTTG CAATTTAATG TCACAGTTTG AAAGCTTAAC ATTCCAACCA
    CAAGCGGGTG CAGGAGAATC AAATGCCATC AGTGAAAAAA AGGAGAGAAT TCTGCCTGAA
    AGAACAAGCC AGGTTAGAAT ACCCAAGGAC TGGCTGGATA AGACCTGCTA CGCAGTTTCA
    CCACCAAGTT CTTCTGAGCC AAATGTTACA GGGAAACGTG GAGAGACCAA GTTAAGGACA
    GAACATAAAA TGGAGTCAGA AAGGGAAAGG TTATCCATGG AATCTGGAGT CCAGACTAAT
    GAGGCACAAC AGTGTCAAGA ACCTCCTGCC CAGAAATCTC TTTTGAAGAC TTCACCCACA
    AATGACAACT CTAAGAAATT ATTTCTGCTT GGGGAGCGTC CATCAAAGCT GGACAGTGAC
    GTCTTAGCAG CTATCGAAGC AGTACAGATC GATCCTCAGA AGTACCCCGC TATTTACAGA
    TGGAAACACG AGGTGCAGTC CTACTCTTCA GCTGACAGAC AAAGTTGGCC AAGTCCAGCA
    TTGAAGGGAA GATTCAGGTC CCAAAGCACA GCTGCTGGCC CACCAAATGC TTCAGTGTAT
    TCTAGTCCAG GAAGAAACAG TCCCATGATG GGAAGTCCAG GGAAACATGG CAATGCTGCC
    TCGTTTTCTC CAGACCCTGG GAGCCCTGGC CGCTACAGTC CTGCCTGTGT CAACCACTCT
    ATATTAAAAC CACGTTATTT TTCAGAGCCT CCTACCCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025011309.1
    CDS32..2833
    Translation

    Target ORF information:

    RefSeq Version XM_025155541.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025155541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGATGCGA TCTTATCCCG CTTGAAGCAA CTCAGCCCTG ATGAGCTTAG AGAGGAACTT 
    GTAAGAGCAG GGCTGAAGTG TGGGCCCGTC ACCTCAACTA CCAGGTTTAT CTTTGAGAAG
    AAGTTGGCCC AGTTTTTACT GGAGCAGCAA GGAACGTTGG AAGAAGGGTC TGCATGGTCA
    GAGGAGGCTG CTGGGAGCGC CCCGCCTGAT GGCACTCACA CAGCTTTGAA AACAACTGCA
    GTGAATCACA GCCATCATGG AAGTGTGTCT GAAGAGGTCG ACTTTGGGTA CTGTGTCGGT
    CTGAACCCTC CAGAAGAAGA AGCTGTGGCA CACACGAACT GTTCAGCTCC AGTGTGTGGG
    GCAGGATATG CTGGTTCACA AGGTGGTGCT CAGACACCAT CCAGAGATCC TCCACTATAC
    TATGGTGTTT GCCCGGTCTA CGATGACATA CTGGCAAGAA ATGAGAGAAT ACATGTTTAT
    GAAGACAGGA AGGAAGCTCT CCAAGCTGTT AAGATGATTA AGGGTTCACG ATTTAAAGCG
    TTTTCAAACA GAGAAGATGC TGAGAAATTT GCTAAAGGAA TCTGTGATTA CTTTCCATCT
    CCAAGCAAGT CCTCTCTGTG TTTGTCACCT ATGAAAACAG GATCACTTAA CAGAGATGGC
    TTATGCTCAC TTGAGATGGA AACTGCTAAC AAGGAAAGGG CCAACAGCTA CAAAAGTCCA
    CGGACCCAGG ACCTCACTGC TAAGCTTCGG AAAGCTGTGG AGAAAGGAGA TATATCAACA
    TTTTCAGAAC TTATTTGGAG TAATCCTCGC TATCTTATTG GATCTGGAGA CAATCCAACA
    GTTGTACAGG AGGGGTGCAG GTACAATGTC ATGCACGTTG CTGCCAAGGA GAACCAGCCT
    GCTGTCTGCC AGCTGTTGTT GGACACTCTG GAAAATCCTG AATTTATGCG GCTTATGTAC
    CCAGATGATG ATAACACAAT GCTGAGGAAT CGCATCCGAT ACATCGTTGA CCTTTACCTC
    AATACACCAG ATAAAATGGG ATTTGATACT CCATTGCATT TTGCCTGCAA GTTTGGGAAT
    TTGGATGTTG TTAATGTGCT TACCTCACAC CCAGCCATTG TAAAAAACCC CAGGAACAAA
    TATGATCAAA CTCCAGCAGA AGTAGTTTGT GAGAGAAGCA AGAATAAATC TGCAGAATTG
    AAAGAAAAAC TGAGAGAGTA TTTGAAAGGT CGCTACTATG TGCCACTTCT GAGAGCAGAA
    GATAACTCCT CAGCTCCTGT CATCGGTGCG CCGTGGTCAC CGGATCAGAC AGACGAGAGC
    CCGCAGGCAG CTCTGTCCAA ATACACGGGC AGCCCCAAGG ATCCGGTGCT GTCTATACGA
    GCTTTCGCAG GGCCCATGAG TCCCTCCAAG GCTGAAGAAT TTCGCAGGCT GTGGAAGACT
    CCACCCCGAG AGAGAGCTGG ATTCTTTCAC AACGTCAGGA AATCTGATCT GGAAAGAGGT
    GTTGAAAGAG TTGGAAGGGA GTTAGCTCAT GAACTGGGGT TCCCGTGGGT TGAATACTGG
    GAATTTCTGG GCTGTTTTGT TGATCTGTCT TCCCAGGAGG GGCTGCGAAA ATTAGAAGAG
    TACCTGAGCC ATCGGGAAAT GAGCGAAAAG GCTCAGCAAG AAACAGGGGA AAATGAAACC
    TGCAACAGAT ACAAAACGCC ACATCCTTCT GGCAAGAGTA AAAAGTGCTG CAATTCTATT
    TCTGTTGGAG CGTTTTTGGA TGAGGATGAT GATGACATGA GCTTAGAAGA AATTAAAAAC
    AGACAAAACG CAGCACGGAA TAACAGCCCA CCTGTTGTGC TGAAGGAACC CAGCATTGAT
    GCTATTGGAG ACGCTGAGCG TGAGATCTTG TCAATGGAGC GTACCAGAAA CATCATAGAA
    ACATCAGCTC AGCCCAGGCA CTACGAGAAG GGGGTTTCTT CCAGCAAAAA TGGATTTTGT
    AATCCTGTGG CCAGTGAAAG GATAATTGGT GACAGAAAGC ATGTGCATGA CGGAGAAGAT
    TGCCTTGTAT CACCTGTTTG CAATTTAATG TCACAGTTTG AAAGCTTAAC ATTCCAACCA
    CAAGCGGGTG CAGGAGAATC AAATGCCATC AGTGAAAAAA AGGAGAGAAT TCTGCCTGAA
    AGAACAAGCC AGGTTAGAAT ACCCAAGGAC TGGCTGGATA AGACCTGCTA CGCAGTTTCA
    CCACCAAGTT CTTCTGAGCC AAATGTTACA GGGAAACGTG GAGAGACCAA GTTAAGGACA
    GAACATAAAA TGGAGTCAGA AAGGGAAAGG TTATCCATGG AATCTGGAGT CCAGACTAAT
    GAGGCACAAC AGTGTCAAGA ACCTCCTGCC CAGAAATCTC TTTTGAAGAC TTCACCCACA
    AATGACAACT CTAAGAAATT ATTTCTGCTT GGGGAGCGTC CATCAAAGCT GGACAGTGAC
    GTCTTAGCAG CTATCGAAGC AGTACAGATC GATCCTCAGA AGTACCCCGC TATTTACAGA
    TGGAAACACG AGGTGCAGTC CTACTCTTCA GCTGACAGAC AAAGTTGGCC AAGTCCAGCA
    TTGAAGGGAA GATTCAGGTC CCAAAGCACA GCTGCTGGCC CACCAAATGC TTCAGTGTAT
    TCTAGTCCAG GAAGAAACAG TCCCATGATG GGAAGTCCAG GGAAACATGG CAATGCTGCC
    TCGTTTTCTC CAGACCCTGG GAGCCCTGGC CGCTACAGTC CTGCCTGTGT CAACCACTCT
    ATATTAAAAC CACGTTATTT TTCAGAGCCT CCTACCCACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144691
    Clone ID Related Accession (Same CDS sequence) XM_025155539.1
    Accession Version XM_025155539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ankyrin repeat and LEM domain-containing protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAGCGCT GGGCCGGGGC GGCCGGCGGC GGCGGCTGGG GCTCGCTGTG GGACGGCGGC 
    TGGGACCGCT GGCCGGCCTG GGAGCTGCTG GTCGCCTGCG CGCTGATCGG CGCGGTGGGC
    TGGCTGCTGC GGCTGCTGGA CGGGCGGACG GGCGGGCGCG GGGCGCCGCC GGAGGGCCTC
    CGTTGCCCGA CCCCGCGCGG CGAGGCGGAG AGAGGGCGGC GCGGCGGGAG CCGCCCAGGT
    GAGATAACGA TGGATGCGAT CTTATCCCGC TTGAAGCAAC TCAGCCCTGA TGAGCTTAGA
    GAGGAACTTG TAAGAGCAGG GCTGAAGTGT GGGCCCGTCA CCTCAACTAC CAGGTTTATC
    TTTGAGAAGA AGTTGGCCCA GTTTTTACTG GAGCAGCAAG GAACGTTGGA AGAAGGGTCT
    GCATGGTCAG AGGAGGCTGC TGGGAGCGCC CCGCCTGATG GCACTCACAC AGCTTTGAAA
    ACAACTGCAG TGAATCACAG CCATCATGGA AGTGTGTCTG AAGAGGTCGA CTTTGGGTAC
    TGTGTCGGTC TGAACCCTCC AGAAGAAGAA GCTGTGGCAC ACACGAACTG TTCAGCTCCA
    GTGTGTGGGG CAGGATATGC TGGTTCACAA GGTGGTGCTC AGACACCATC CAGAGATCCT
    CCACTATACT ATGGTGTTTG CCCGGTCTAC GATGACATAC TGGCAAGAAA TGAGAGAATA
    CATGTTTATG AAGACAGGAA GGAAGCTCTC CAAGCTGTTA AGATGATTAA GGGTTCACGA
    TTTAAAGCGT TTTCAAACAG AGAAGATGCT GAGAAATTTG CTAAAGGAAT CTGTGATTAC
    TTTCCATCTC CAAGCAAGTC CTCTCTGTGT TTGTCACCTA TGAAAACAGG ATCACTTAAC
    AGAGATGGCT TATGCTCACT TGAGATGGAA ACTGCTAACA AGGAAAGGGC CAACAGCTAC
    AAAAGTCCAC GGACCCAGGA CCTCACTGCT AAGCTTCGGA AAGCTGTGGA GAAAGGAGAT
    ATATCAACAT TTTCAGAACT TATTTGGAGT AATCCTCGCT ATCTTATTGG ATCTGGAGAC
    AATCCAACAG TTGTACAGGA GGGGTGCAGG TACAATGTCA TGCACGTTGC TGCCAAGGAG
    AACCAGCCTG CTGTCTGCCA GCTGTTGTTG GACACTCTGG AAAATCCTGA ATTTATGCGG
    CTTATGTACC CAGATGATGA TAACACAATG CTGAGGAATC GCATCCGATA CATCGTTGAC
    CTTTACCTCA ATACACCAGA TAAAATGGGA TTTGATACTC CATTGCATTT TGCCTGCAAG
    TTTGGGAATT TGGATGTTGT TAATGTGCTT ACCTCACACC CAGCCATTGT AAAAAACCCC
    AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AGAGAAGCAA GAATAAATCT
    GCAGAATTGA AAGAAAAACT GAGAGAGTAT TTGAAAGGTC GCTACTATGT GCCACTTCTG
    AGAGCAGAAG ATAACTCCTC AGCTCCTGTC ATCGGTGCGC CGTGGTCACC GGATCAGACA
    GACGAGAGCC CGCAGGCAGC TCTGTCCAAA TACACGGGCA GCCCCAAGGA TCCGGTGCTG
    TCTATACGAG CTTTCGCAGG GCCCATGAGT CCCTCCAAGG CTGAAGAATT TCGCAGGCTG
    TGGAAGACTC CACCCCGAGA GAGAGCTGGA TTCTTTCACA ACGTCAGGAA ATCTGATCTG
    GAAAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AACTGGGGTT CCCGTGGGTT
    GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GCTGCGAAAA
    TTAGAAGAGT ACCTGAGCCA TCGGGAAATG AGCGAAAAGG CTCAGCAAGA AACAGGGGAA
    AATGAAACCT GCAACAGATA CAAAACGCCA CATCCTTCTG GCAAGAGTAA AAAGTGCTGC
    AATTCTATTT CTGTTGGAGC GTTTTTGGAT GAGGATGATG ATGACATGAG CTTAGAAGAA
    ATTAAAAACA GACAAAACGC AGCACGGAAT AACAGCCCAC CTGTTGTGCT GAAGGAACCC
    AGCATTGATG CTATTGGAGA CGCTGAGCGT GAGATCTTGT CAATGGAGCG TACCAGAAAC
    ATCATAGAAA CATCAGCTCA GCCCAGGCAC TACGAGAAGG GGGTTTCTTC CAGCAAAAAT
    GGATTTTGTA ATCCTGTGGC CAGTGAAAGG ATAATTGGTG ACAGAAAGCA TGTGCATGAC
    GGAGAAGATT GCCTTGTATC ACCTGTTTGC AATTTAATGT CACAGTTTGA AAGCTTAACA
    TTCCAACCAC AAGCGGGTGC AGGAGAATCA AATGCCATCA GTGAAAAAAA GGAGAGAATT
    CTGCCTGAAA GAACAAGCCA GGTTAGAATA CCCAAGGACT GGCTGGATAA GACCTGCTAC
    GCAGTTTCAC CACCAAGTTC TTCTGAGCCA AATGTTACAG GGAAACGTGG AGAGACCAAG
    TTAAGGACAG AACATAAAAT GGAGTCAGAA AGGGAAAGGT TATCCATGGA ATCTGGAGTC
    CAGACTAATG AGGCACAACA GTGTCAAGAA CCTCCTGCCC AGAAATCTCT TTTGAAGACT
    TCACCCACAA ATGACAACTC TAAGAAATTA TTTCTGCTTG GGGAGCGTCC ATCAAAGCTG
    GACAGTGACG TCTTAGCAGC TATCGAAGCA GTACAGATCG ATCCTCAGAA GTACCCCGCT
    ATTTACAGAT GGAAACACGA GGTGCAGTCC TACTCTTCAG CTGACAGACA AAGTTGGCCA
    AGTCCAGCAT TGAAGGGAAG ATTCAGGTCC CAAAGCACAG CTGCTGGCCC ACCAAATGCT
    TCAGTGTATT CTAGTCCAGG AAGAAACAGT CCCATGATGG GAAGTCCAGG GAAACATGGC
    AATGCTGCCT CGTTTTCTCC AGACCCTGGG AGCCCTGGCC GCTACAGTCC TGCCTGTGTC
    AACCACTCTA TATTAAAACC ACGTTATTTT TCAGAGCCTC CTACCCACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025011307.1
    CDS34..3084
    Translation

    Target ORF information:

    RefSeq Version XM_025155539.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025155539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAGCGCT GGGCCGGGGC GGCCGGCGGC GGCGGCTGGG GCTCGCTGTG GGACGGCGGC 
    TGGGACCGCT GGCCGGCCTG GGAGCTGCTG GTCGCCTGCG CGCTGATCGG CGCGGTGGGC
    TGGCTGCTGC GGCTGCTGGA CGGGCGGACG GGCGGGCGCG GGGCGCCGCC GGAGGGCCTC
    CGTTGCCCGA CCCCGCGCGG CGAGGCGGAG AGAGGGCGGC GCGGCGGGAG CCGCCCAGGT
    GAGATAACGA TGGATGCGAT CTTATCCCGC TTGAAGCAAC TCAGCCCTGA TGAGCTTAGA
    GAGGAACTTG TAAGAGCAGG GCTGAAGTGT GGGCCCGTCA CCTCAACTAC CAGGTTTATC
    TTTGAGAAGA AGTTGGCCCA GTTTTTACTG GAGCAGCAAG GAACGTTGGA AGAAGGGTCT
    GCATGGTCAG AGGAGGCTGC TGGGAGCGCC CCGCCTGATG GCACTCACAC AGCTTTGAAA
    ACAACTGCAG TGAATCACAG CCATCATGGA AGTGTGTCTG AAGAGGTCGA CTTTGGGTAC
    TGTGTCGGTC TGAACCCTCC AGAAGAAGAA GCTGTGGCAC ACACGAACTG TTCAGCTCCA
    GTGTGTGGGG CAGGATATGC TGGTTCACAA GGTGGTGCTC AGACACCATC CAGAGATCCT
    CCACTATACT ATGGTGTTTG CCCGGTCTAC GATGACATAC TGGCAAGAAA TGAGAGAATA
    CATGTTTATG AAGACAGGAA GGAAGCTCTC CAAGCTGTTA AGATGATTAA GGGTTCACGA
    TTTAAAGCGT TTTCAAACAG AGAAGATGCT GAGAAATTTG CTAAAGGAAT CTGTGATTAC
    TTTCCATCTC CAAGCAAGTC CTCTCTGTGT TTGTCACCTA TGAAAACAGG ATCACTTAAC
    AGAGATGGCT TATGCTCACT TGAGATGGAA ACTGCTAACA AGGAAAGGGC CAACAGCTAC
    AAAAGTCCAC GGACCCAGGA CCTCACTGCT AAGCTTCGGA AAGCTGTGGA GAAAGGAGAT
    ATATCAACAT TTTCAGAACT TATTTGGAGT AATCCTCGCT ATCTTATTGG ATCTGGAGAC
    AATCCAACAG TTGTACAGGA GGGGTGCAGG TACAATGTCA TGCACGTTGC TGCCAAGGAG
    AACCAGCCTG CTGTCTGCCA GCTGTTGTTG GACACTCTGG AAAATCCTGA ATTTATGCGG
    CTTATGTACC CAGATGATGA TAACACAATG CTGAGGAATC GCATCCGATA CATCGTTGAC
    CTTTACCTCA ATACACCAGA TAAAATGGGA TTTGATACTC CATTGCATTT TGCCTGCAAG
    TTTGGGAATT TGGATGTTGT TAATGTGCTT ACCTCACACC CAGCCATTGT AAAAAACCCC
    AGGAACAAAT ATGATCAAAC TCCAGCAGAA GTAGTTTGTG AGAGAAGCAA GAATAAATCT
    GCAGAATTGA AAGAAAAACT GAGAGAGTAT TTGAAAGGTC GCTACTATGT GCCACTTCTG
    AGAGCAGAAG ATAACTCCTC AGCTCCTGTC ATCGGTGCGC CGTGGTCACC GGATCAGACA
    GACGAGAGCC CGCAGGCAGC TCTGTCCAAA TACACGGGCA GCCCCAAGGA TCCGGTGCTG
    TCTATACGAG CTTTCGCAGG GCCCATGAGT CCCTCCAAGG CTGAAGAATT TCGCAGGCTG
    TGGAAGACTC CACCCCGAGA GAGAGCTGGA TTCTTTCACA ACGTCAGGAA ATCTGATCTG
    GAAAGAGGTG TTGAAAGAGT TGGAAGGGAG TTAGCTCATG AACTGGGGTT CCCGTGGGTT
    GAATACTGGG AATTTCTGGG CTGTTTTGTT GATCTGTCTT CCCAGGAGGG GCTGCGAAAA
    TTAGAAGAGT ACCTGAGCCA TCGGGAAATG AGCGAAAAGG CTCAGCAAGA AACAGGGGAA
    AATGAAACCT GCAACAGATA CAAAACGCCA CATCCTTCTG GCAAGAGTAA AAAGTGCTGC
    AATTCTATTT CTGTTGGAGC GTTTTTGGAT GAGGATGATG ATGACATGAG CTTAGAAGAA
    ATTAAAAACA GACAAAACGC AGCACGGAAT AACAGCCCAC CTGTTGTGCT GAAGGAACCC
    AGCATTGATG CTATTGGAGA CGCTGAGCGT GAGATCTTGT CAATGGAGCG TACCAGAAAC
    ATCATAGAAA CATCAGCTCA GCCCAGGCAC TACGAGAAGG GGGTTTCTTC CAGCAAAAAT
    GGATTTTGTA ATCCTGTGGC CAGTGAAAGG ATAATTGGTG ACAGAAAGCA TGTGCATGAC
    GGAGAAGATT GCCTTGTATC ACCTGTTTGC AATTTAATGT CACAGTTTGA AAGCTTAACA
    TTCCAACCAC AAGCGGGTGC AGGAGAATCA AATGCCATCA GTGAAAAAAA GGAGAGAATT
    CTGCCTGAAA GAACAAGCCA GGTTAGAATA CCCAAGGACT GGCTGGATAA GACCTGCTAC
    GCAGTTTCAC CACCAAGTTC TTCTGAGCCA AATGTTACAG GGAAACGTGG AGAGACCAAG
    TTAAGGACAG AACATAAAAT GGAGTCAGAA AGGGAAAGGT TATCCATGGA ATCTGGAGTC
    CAGACTAATG AGGCACAACA GTGTCAAGAA CCTCCTGCCC AGAAATCTCT TTTGAAGACT
    TCACCCACAA ATGACAACTC TAAGAAATTA TTTCTGCTTG GGGAGCGTCC ATCAAAGCTG
    GACAGTGACG TCTTAGCAGC TATCGAAGCA GTACAGATCG ATCCTCAGAA GTACCCCGCT
    ATTTACAGAT GGAAACACGA GGTGCAGTCC TACTCTTCAG CTGACAGACA AAGTTGGCCA
    AGTCCAGCAT TGAAGGGAAG ATTCAGGTCC CAAAGCACAG CTGCTGGCCC ACCAAATGCT
    TCAGTGTATT CTAGTCCAGG AAGAAACAGT CCCATGATGG GAAGTCCAGG GAAACATGGC
    AATGCTGCCT CGTTTTCTCC AGACCCTGGG AGCCCTGGCC GCTACAGTCC TGCCTGTGTC
    AACCACTCTA TATTAAAACC ACGTTATTTT TCAGAGCCTC CTACCCACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa15430
    Clone ID Related Accession (Same CDS sequence) XM_004945490.2
    Accession Version XM_004945490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ankyrin repeat and LEM domain-containing protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004945490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGCCG AGCTGCGGGC AGCGGAAGGG CGACGCACCG AGCGGGAGAG CGGAGCGCGG 
    CGCGGAGCTC CGGCAGCAGG GGGCGCCGGC GGCCCCCAGA GGGCTGCGTG CAGAAGCGGC
    GTTTTTCAGC GCGAAAACCC TCCCCGCGGT GAGCTCCGAG CGATCCCCGC TGCGCTCGGC
    GCCTCCCACG GGGCCCCGCC GCCCTTCAGG CGCCGAACTT TCGTTTTGCA CGGGACTTCC
    GCTCAGCGGG CGGGCTGCGG GAGCGCCGAA GGCCGCGGGG ATCAGCGCCG GAGAAGCGGA
    GGCCGCGGCG GGGCGCTCCG TCCAGAAGGA AACGCGGAGG TAAGCGGCGG GGCCGCGAGA
    CGGGCAGCAC CGCGCGGGGC TCCGCGGCCG CTATCGCCTC ACGCGTCGGC GGGAACGCAC
    GGCGAGCGGC CCGGCGCTCC CGCCATGCCA CGCGGCCGCG TTGCCGCGCG ACGGGCGCTG
    CTCTGCTGTC AGGCGCGGCG CGGGGCGGGG CGGAGGGCCG GAAGCCGGGA GGCCGCTGAG
    GTACCGACCC CGGAGGCCGG CGGCTGCGCG CGGCGGGCGG GCCGGGGCAT GGAGCGCTGG
    GCCGGGGCGG CCGGCGGCGG CGGCTGGGGC TCGCTGTGGG ACGGCGGCTG GGACCGCTGG
    CCGGCCTGGG AGCTGCTGGT CGCCTGCGCG CTGATCGGCG CGGTGGGCTG GCTGCTGCGG
    CTGCTGGACG GGCGGACGGG CGGGCGCGGG GCGCCGCCGG AGGGCCTCCG TTGCCCGACC
    CCGCGCGGCG AGGCGGAGAG AGGGCGGCGC GGCGGGAGCC GCCCAGGTGA GATAACGATG
    GATGCGATCT TATCCCGCTT GAAGCAACTC AGCCCTGATG AGCTTAGAGA GGAACTTGTA
    AGAGCAGGGC TGAAGTGTGG GCCCGTCACC TCAACTACCA GGTTTATCTT TGAGAAGAAG
    TTGGCCCAGT TTTTACTGGA GCAGCAAGGA ACGTTGGAAG AAGGGTCTGC ATGGTCAGAG
    GAGGCTGCTG GGAGCGCCCC GCCTGATGGC ACTCACACAG CTTTGAAAAC AACTGCAGTG
    AATCACAGCC ATCATGGAAG TGTGTCTGAA GAGGTCGACT TTGGGTACTG TGTCGGTCTG
    AACCCTCCAG AAGAAGAAGC TGTGGCACAC ACGAACTGTT CAGCTCCAGT GTGTGGGGCA
    GGATATGCTG GTTCACAAGG TGGTGCTCAG ACACCATCCA GAGATCCTCC ACTATACTAT
    GGTGTTTGCC CGGTCTACGA TGACATACTG GCAAGAAATG AGAGAATACA TGTTTATGAA
    GACAGGAAGG AAGCTCTCCA AGCTGTTAAG ATGATTAAGG GTTCACGATT TAAAGCGTTT
    TCAAACAGAG AAGATGCTGA GAAATTTGCT AAAGGAATCT GTGATTACTT TCCATCTCCA
    AGCAAGTCCT CTCTGTGTTT GTCACCTATG AAAACAGGAT CACTTAACAG AGATGGCTTA
    TGCTCACTTG AGATGGAAAC TGCTAACAAG GAAAGGGCCA ACAGCTACAA AAGTCCACGG
    ACCCAGGACC TCACTGCTAA GCTTCGGAAA GCTGTGGAGA AAGGAGATAT ATCAACATTT
    TCAGAACTTA TTTGGAGTAA TCCTCGCTAT CTTATTGGAT CTGGAGACAA TCCAACAGTT
    GTACAGGAGG GGTGCAGGTA CAATGTCATG CACGTTGCTG CCAAGGAGAA CCAGCCTGCT
    GTCTGCCAGC TGTTGTTGGA CACTCTGGAA AATCCTGAAT TTATGCGGCT TATGTACCCA
    GATGATGATA ACACAATGCT GAGGAATCGC ATCCGATACA TCGTTGACCT TTACCTCAAT
    ACACCAGATA AAATGGGATT TGATACTCCA TTGCATTTTG CCTGCAAGTT TGGGAATTTG
    GATGTTGTTA ATGTGCTTAC CTCACACCCA GCCATTGTAA AAAACCCCAG GAACAAATAT
    GATCAAACTC CAGCAGAAGT AGTTTGTGAG AGAAGCAAGA ATAAATCTGC AGAATTGAAA
    GAAAAACTGA GAGAGTATTT GAAAGGTCGC TACTATGTGC CACTTCTGAG AGCAGAAGAT
    AACTCCTCAG CTCCTGTCAT CGGTGCGCCG TGGTCACCGG ATCAGACAGA CGAGAGCCCG
    CAGGCAGCTC TGTCCAAATA CACGGGCAGC CCCAAGGATC CGGTGCTGTC TATACGAGCT
    TTCGCAGGGC CCATGAGTCC CTCCAAGGCT GAAGAATTTC GCAGGCTGTG GAAGACTCCA
    CCCCGAGAGA GAGCTGGATT CTTTCACAAC GTCAGGAAAT CTGATCTGGA AAGAGGTGTT
    GAAAGAGTTG GAAGGGAGTT AGCTCATGAA CTGGGGTTCC CGTGGGTTGA ATACTGGGAA
    TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAGGGGC TGCGAAAATT AGAAGAGTAC
    CTGAGCCATC GGGAAATGAG CGAAAAGGCT CAGCAAGAAA CAGGGGAAAA TGAAACCTGC
    AACAGATACA AAACGCCACA TCCTTCTGGC AAGAGTAAAA AGTGCTGCAA TTCTATTTCT
    GTTGGAGCGT TTTTGGATGA GGATGATGAT GACATGAGCT TAGAAGAAAT TAAAAACAGA
    CAAAACGCAG CACGGAATAA CAGCCCACCT GTTGTGCTGA AGGAACCCAG CATTGATGCT
    ATTGGAGACG CTGAGCGTGA GATCTTGTCA ATGGAGCGTA CCAGAAACAT CATAGAAACA
    TCAGCTCAGC CCAGGCACTA CGAGAAGGGG GTTTCTTCCA GCAAAAATGG ATTTTGTAAT
    CCTGTGGCCA GTGAAAGGAT AATTGGTGAC AGAAAGCATG TGCATGACGG AGAAGATTGC
    CTTGTATCAC CTGTTTGCAA TTTAATGTCA CAGTTTGAAA GCTTAACATT CCAACCACAA
    GCGGGTGCAG GAGAATCAAA TGCCATCAGT GAAAAAAAGG AGAGAATTCT GCCTGAAAGA
    ACAAGCCAGG TTAGAATACC CAAGGACTGG CTGGATAAGA CCTGCTACGC AGTTTCACCA
    CCAAGTTCTT CTGAGCCAAA TGTTACAGGG AAACGTGGAG AGACCAAGTT AAGGACAGAA
    CATAAAATGG AGTCAGAAAG GGAAAGGTTA TCCATGGAAT CTGGAGTCCA GACTAATGAG
    GCACAACAGT GTCAAGAACC TCCTGCCCAG AAATCTCTTT TGAAGACTTC ACCCACAAAT
    GACAACTCTA AGAAATTATT TCTGCTTGGG GAGCGTCCAT CAAAGCTGGA CAGTGACGTC
    TTAGCAGCTA TCGAAGCAGT ACAGATCGAT CCTCAGAAGT ACCCCGCTAT TTACAGATGG
    AAACACGAGG TGCAGTCCTA CTCTTCAGCT GACAGACAAA GGGGGGATGT GCAACTGGAA
    AAGCCTACAA CAAAGCCTCG TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004945547.1
    CDS1..3447
    Translation

    Target ORF information:

    RefSeq Version XM_004945490.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004945490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGCCG AGCTGCGGGC AGCGGAAGGG CGACGCACCG AGCGGGAGAG CGGAGCGCGG 
    CGCGGAGCTC CGGCAGCAGG GGGCGCCGGC GGCCCCCAGA GGGCTGCGTG CAGAAGCGGC
    GTTTTTCAGC GCGAAAACCC TCCCCGCGGT GAGCTCCGAG CGATCCCCGC TGCGCTCGGC
    GCCTCCCACG GGGCCCCGCC GCCCTTCAGG CGCCGAACTT TCGTTTTGCA CGGGACTTCC
    GCTCAGCGGG CGGGCTGCGG GAGCGCCGAA GGCCGCGGGG ATCAGCGCCG GAGAAGCGGA
    GGCCGCGGCG GGGCGCTCCG TCCAGAAGGA AACGCGGAGG TAAGCGGCGG GGCCGCGAGA
    CGGGCAGCAC CGCGCGGGGC TCCGCGGCCG CTATCGCCTC ACGCGTCGGC GGGAACGCAC
    GGCGAGCGGC CCGGCGCTCC CGCCATGCCA CGCGGCCGCG TTGCCGCGCG ACGGGCGCTG
    CTCTGCTGTC AGGCGCGGCG CGGGGCGGGG CGGAGGGCCG GAAGCCGGGA GGCCGCTGAG
    GTACCGACCC CGGAGGCCGG CGGCTGCGCG CGGCGGGCGG GCCGGGGCAT GGAGCGCTGG
    GCCGGGGCGG CCGGCGGCGG CGGCTGGGGC TCGCTGTGGG ACGGCGGCTG GGACCGCTGG
    CCGGCCTGGG AGCTGCTGGT CGCCTGCGCG CTGATCGGCG CGGTGGGCTG GCTGCTGCGG
    CTGCTGGACG GGCGGACGGG CGGGCGCGGG GCGCCGCCGG AGGGCCTCCG TTGCCCGACC
    CCGCGCGGCG AGGCGGAGAG AGGGCGGCGC GGCGGGAGCC GCCCAGGTGA GATAACGATG
    GATGCGATCT TATCCCGCTT GAAGCAACTC AGCCCTGATG AGCTTAGAGA GGAACTTGTA
    AGAGCAGGGC TGAAGTGTGG GCCCGTCACC TCAACTACCA GGTTTATCTT TGAGAAGAAG
    TTGGCCCAGT TTTTACTGGA GCAGCAAGGA ACGTTGGAAG AAGGGTCTGC ATGGTCAGAG
    GAGGCTGCTG GGAGCGCCCC GCCTGATGGC ACTCACACAG CTTTGAAAAC AACTGCAGTG
    AATCACAGCC ATCATGGAAG TGTGTCTGAA GAGGTCGACT TTGGGTACTG TGTCGGTCTG
    AACCCTCCAG AAGAAGAAGC TGTGGCACAC ACGAACTGTT CAGCTCCAGT GTGTGGGGCA
    GGATATGCTG GTTCACAAGG TGGTGCTCAG ACACCATCCA GAGATCCTCC ACTATACTAT
    GGTGTTTGCC CGGTCTACGA TGACATACTG GCAAGAAATG AGAGAATACA TGTTTATGAA
    GACAGGAAGG AAGCTCTCCA AGCTGTTAAG ATGATTAAGG GTTCACGATT TAAAGCGTTT
    TCAAACAGAG AAGATGCTGA GAAATTTGCT AAAGGAATCT GTGATTACTT TCCATCTCCA
    AGCAAGTCCT CTCTGTGTTT GTCACCTATG AAAACAGGAT CACTTAACAG AGATGGCTTA
    TGCTCACTTG AGATGGAAAC TGCTAACAAG GAAAGGGCCA ACAGCTACAA AAGTCCACGG
    ACCCAGGACC TCACTGCTAA GCTTCGGAAA GCTGTGGAGA AAGGAGATAT ATCAACATTT
    TCAGAACTTA TTTGGAGTAA TCCTCGCTAT CTTATTGGAT CTGGAGACAA TCCAACAGTT
    GTACAGGAGG GGTGCAGGTA CAATGTCATG CACGTTGCTG CCAAGGAGAA CCAGCCTGCT
    GTCTGCCAGC TGTTGTTGGA CACTCTGGAA AATCCTGAAT TTATGCGGCT TATGTACCCA
    GATGATGATA ACACAATGCT GAGGAATCGC ATCCGATACA TCGTTGACCT TTACCTCAAT
    ACACCAGATA AAATGGGATT TGATACTCCA TTGCATTTTG CCTGCAAGTT TGGGAATTTG
    GATGTTGTTA ATGTGCTTAC CTCACACCCA GCCATTGTAA AAAACCCCAG GAACAAATAT
    GATCAAACTC CAGCAGAAGT AGTTTGTGAG AGAAGCAAGA ATAAATCTGC AGAATTGAAA
    GAAAAACTGA GAGAGTATTT GAAAGGTCGC TACTATGTGC CACTTCTGAG AGCAGAAGAT
    AACTCCTCAG CTCCTGTCAT CGGTGCGCCG TGGTCACCGG ATCAGACAGA CGAGAGCCCG
    CAGGCAGCTC TGTCCAAATA CACGGGCAGC CCCAAGGATC CGGTGCTGTC TATACGAGCT
    TTCGCAGGGC CCATGAGTCC CTCCAAGGCT GAAGAATTTC GCAGGCTGTG GAAGACTCCA
    CCCCGAGAGA GAGCTGGATT CTTTCACAAC GTCAGGAAAT CTGATCTGGA AAGAGGTGTT
    GAAAGAGTTG GAAGGGAGTT AGCTCATGAA CTGGGGTTCC CGTGGGTTGA ATACTGGGAA
    TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAGGGGC TGCGAAAATT AGAAGAGTAC
    CTGAGCCATC GGGAAATGAG CGAAAAGGCT CAGCAAGAAA CAGGGGAAAA TGAAACCTGC
    AACAGATACA AAACGCCACA TCCTTCTGGC AAGAGTAAAA AGTGCTGCAA TTCTATTTCT
    GTTGGAGCGT TTTTGGATGA GGATGATGAT GACATGAGCT TAGAAGAAAT TAAAAACAGA
    CAAAACGCAG CACGGAATAA CAGCCCACCT GTTGTGCTGA AGGAACCCAG CATTGATGCT
    ATTGGAGACG CTGAGCGTGA GATCTTGTCA ATGGAGCGTA CCAGAAACAT CATAGAAACA
    TCAGCTCAGC CCAGGCACTA CGAGAAGGGG GTTTCTTCCA GCAAAAATGG ATTTTGTAAT
    CCTGTGGCCA GTGAAAGGAT AATTGGTGAC AGAAAGCATG TGCATGACGG AGAAGATTGC
    CTTGTATCAC CTGTTTGCAA TTTAATGTCA CAGTTTGAAA GCTTAACATT CCAACCACAA
    GCGGGTGCAG GAGAATCAAA TGCCATCAGT GAAAAAAAGG AGAGAATTCT GCCTGAAAGA
    ACAAGCCAGG TTAGAATACC CAAGGACTGG CTGGATAAGA CCTGCTACGC AGTTTCACCA
    CCAAGTTCTT CTGAGCCAAA TGTTACAGGG AAACGTGGAG AGACCAAGTT AAGGACAGAA
    CATAAAATGG AGTCAGAAAG GGAAAGGTTA TCCATGGAAT CTGGAGTCCA GACTAATGAG
    GCACAACAGT GTCAAGAACC TCCTGCCCAG AAATCTCTTT TGAAGACTTC ACCCACAAAT
    GACAACTCTA AGAAATTATT TCTGCTTGGG GAGCGTCCAT CAAAGCTGGA CAGTGACGTC
    TTAGCAGCTA TCGAAGCAGT ACAGATCGAT CCTCAGAAGT ACCCCGCTAT TTACAGATGG
    AAACACGAGG TGCAGTCCTA CTCTTCAGCT GACAGACAAA GGGGGGATGT GCAACTGGAA
    AAGCCTACAA CAAAGCCTCG TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.