SMURF1 cDNA ORF clone, Gallus gallus(chicken)

The following SMURF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa42320 XM_015294373.2
Latest version!
Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42321 XM_015294374.2
Latest version!
Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42322 XM_414794.6
Latest version!
Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42323 XM_015294375.2
Latest version!
Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42324 XM_015294376.2
Latest version!
Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OGa42323 XM_015294375.1 Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42321 XM_015294374.1 Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42320 XM_015294373.1 Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42324 XM_015294376.1 Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa42322 XM_414794.5 Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa42320
    Clone ID Related Accession (Same CDS sequence) XM_015294373.1 , XM_015294373.2
    Accession Version XM_015294373.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG TTCTACAGGC
    GCTGCAGGAC CCAGACTGTT TACCATCCAT TTAATAGATG CAAACACAGA CAACCTTCCG
    AAAGCCCACA CTTGCTTTAA CCGGATTGAC ATTCCGCCTT ATGAGTCATA TGAGAAGCTT
    TACGAGAAGC TGTTGACAGC TGTGGAAGAG ACATGTGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149859.1
    CDS123..2336
    Translation

    Target ORF information:

    RefSeq Version XM_015294373.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG TTCTACAGGC
    GCTGCAGGAC CCAGACTGTT TACCATCCAT TTAATAGATG CAAACACAGA CAACCTTCCG
    AAAGCCCACA CTTGCTTTAA CCGGATTGAC ATTCCGCCTT ATGAGTCATA TGAGAAGCTT
    TACGAGAAGC TGTTGACAGC TGTGGAAGAG ACATGTGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42320
    Clone ID Related Accession (Same CDS sequence) XM_015294373.1 , XM_015294373.2
    Accession Version XM_015294373.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG TTCTACAGGC
    GCTGCAGGAC CCAGACTGTT TACCATCCAT TTAATAGATG CAAACACAGA CAACCTTCCG
    AAAGCCCACA CTTGCTTTAA CCGGATTGAC ATTCCGCCTT ATGAGTCATA TGAGAAGCTT
    TACGAGAAGC TGTTGACAGC TGTGGAAGAG ACATGTGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149859.1
    CDS130..2343
    Translation

    Target ORF information:

    RefSeq Version XM_015294373.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294373.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG TTCTACAGGC
    GCTGCAGGAC CCAGACTGTT TACCATCCAT TTAATAGATG CAAACACAGA CAACCTTCCG
    AAAGCCCACA CTTGCTTTAA CCGGATTGAC ATTCCGCCTT ATGAGTCATA TGAGAAGCTT
    TACGAGAAGC TGTTGACAGC TGTGGAAGAG ACATGTGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42321
    Clone ID Related Accession (Same CDS sequence) XM_015294374.2 , XM_015294374.1
    Accession Version XM_015294374.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG CGCTGCAGGA
    CCCAGACTGT TTACCATCCA TTTAATAGAT GCAAACACAG ACAACCTTCC GAAAGCCCAC
    ACTTGCTTTA ACCGGATTGA CATTCCGCCT TATGAGTCAT ATGAGAAGCT TTACGAGAAG
    CTGTTGACAG CTGTGGAAGA GACATGTGGG TTTGCTGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149860.1
    CDS130..2334
    Translation

    Target ORF information:

    RefSeq Version XM_015294374.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294374.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG CGCTGCAGGA
    CCCAGACTGT TTACCATCCA TTTAATAGAT GCAAACACAG ACAACCTTCC GAAAGCCCAC
    ACTTGCTTTA ACCGGATTGA CATTCCGCCT TATGAGTCAT ATGAGAAGCT TTACGAGAAG
    CTGTTGACAG CTGTGGAAGA GACATGTGGG TTTGCTGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42321
    Clone ID Related Accession (Same CDS sequence) XM_015294374.2 , XM_015294374.1
    Accession Version XM_015294374.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG CGCTGCAGGA
    CCCAGACTGT TTACCATCCA TTTAATAGAT GCAAACACAG ACAACCTTCC GAAAGCCCAC
    ACTTGCTTTA ACCGGATTGA CATTCCGCCT TATGAGTCAT ATGAGAAGCT TTACGAGAAG
    CTGTTGACAG CTGTGGAAGA GACATGTGGG TTTGCTGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149860.1
    CDS123..2327
    Translation

    Target ORF information:

    RefSeq Version XM_015294374.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGT
    TTCCCCATTT TCAGAACGGT TTATGAAGAT TCTGGACCAG GGAGGCCACT GAGCTGTTTT
    ATGGAAGAAC CAGCACCCTA CACAGACACT ACAGGGGCTG CAGGTGGAGG CAATTGTCGC
    TTTGTAGAGT CCCCCAGTCA AGATCAGAGA CTTCAGGCAC AGAGACTTCG GACTCCTGAA
    GTCAGAGGTC ATGTACAGAC ACCTCAGAAC AGACCACACG GTCACCAGTC TCCTGACCTA
    CCTGAAGGCT ATGAACAAAG AACAACGGTG CAGGGCCAGG TTTATTTTTT GCACACGCAG
    ACTGGAGTTA GCACATGGCA TGACCCTAGG ATACCAAGAG ACCTTAACAG TGTGAATTGT
    GATGAACTGG GACCTCTGCC TCCAGGTTGG GAAGTTAGAA GTACAGTTTC AGGAAGAATA
    TATTTTGTAG ATCATAACAA CAGAACCACG CAGTTCACAG ATCCACGACT ACATCACATC
    ATGAATCACC AATGCCAGCT AAAAGAGCCC AGCCAGCAGC CTCTGCCAGC TCCAAATGAG
    GGGTCTGTGG AGGAGGGTGA AGAGTTGCCT GCACAGAGAT ATGAAAGAGA CTTGGTGCAA
    AAGCTGAAAG TTCTTAGACA TGAGCTCTCC CTTCAACAAC CACAGGCAGG TCACTGCCGA
    ATTGAAGTAT CCAGAGAGGA AATATTTGAG GAATCCTACC GTCAGATAAT GAAGATGAGG
    CCAAAGGATT TGAAAAAGAG GCTTATGGTA AAATTTCGAG GCGAGGAAGG CCTGGATTAT
    GGAGGGGTAG CAAGAGAATG GTTGTATTTA CTGTGCCATG AAATGTTGAA TCCATACTAT
    GGACTCTTCC AGTACTCTAC AGATAATATT TACATGCTGC AAATCAATCC AGACTCCTCT
    ATCAATCCTG ACCATTTGTC TTATTTCCAT TTTGTTGGGC GAATAATGGG TTTGGCTGTG
    TTCCATGGAC ACTATATCAA TGGGGGGTTC ACGGTTCCCT TTTATAAACA GCTCCTGGGG
    AAACCCATTC AGCTCTCTGA TCTGGAATCT GTTGACCCAG AACTACACAA AAGTTTAGTT
    TGGATTCTAG AGAACGATAT CACCCCGGTT CTGGACCATA CTTTTTGTGT GGAACACAAT
    GCTTTTGGGA GGATTTTACA GCACGAACTC AAACCAAATG GCAGGAACAT TCCAGTGACA
    GAAGAGAACA AGAAAGAATA TGTCAGGTTG TATGTGAACT GGCGATTTAT GAGAGGCATA
    GAAGCACAGT TCTTGGCTCT GCAGAAGGGT TTTAACGAAC TCATTCCCCA GCATCTCCTC
    AAGCCTTTTG ACCAGAAGGA ACTTGAGCTA ATCATAGGAG GCCTGGATAA GATAGACCTG
    AATGACTGGA AGTCAAACAC ACGTCTAAAG CACTGCATGG CAGATAGCAA TATTGTGAAG
    TGGTTCTGGC AAGCGGTGGA AACATTTGAT GAAGAAAGAA GGGCAAGGCT TTTGCAGTTT
    GTGACAGGTT CAACACGTGT CCCACTTCAA GGTTTCAAGG CTTTACAAGG CGCTGCAGGA
    CCCAGACTGT TTACCATCCA TTTAATAGAT GCAAACACAG ACAACCTTCC GAAAGCCCAC
    ACTTGCTTTA ACCGGATTGA CATTCCGCCT TATGAGTCAT ATGAGAAGCT TTACGAGAAG
    CTGTTGACAG CTGTGGAAGA GACATGTGGG TTTGCTGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42322
    Clone ID Related Accession (Same CDS sequence) XM_414794.6 , XM_414794.5
    Accession Version XM_414794.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_414794.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGTTCTA CAGGCGCTGC AGGACCCAGA
    CTGTTTACCA TCCATTTAAT AGATGCAAAC ACAGACAACC TTCCGAAAGC CCACACTTGC
    TTTAACCGGA TTGACATTCC GCCTTATGAG TCATATGAGA AGCTTTACGA GAAGCTGTTG
    ACAGCTGTGG AAGAGACATG TGGGTTTGCT GTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_414794.3
    CDS129..2327
    Translation

    Target ORF information:

    RefSeq Version XM_414794.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_414794.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGTTCTA CAGGCGCTGC AGGACCCAGA
    CTGTTTACCA TCCATTTAAT AGATGCAAAC ACAGACAACC TTCCGAAAGC CCACACTTGC
    TTTAACCGGA TTGACATTCC GCCTTATGAG TCATATGAGA AGCTTTACGA GAAGCTGTTG
    ACAGCTGTGG AAGAGACATG TGGGTTTGCT GTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42322
    Clone ID Related Accession (Same CDS sequence) XM_414794.6 , XM_414794.5
    Accession Version XM_414794.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_414794.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGTTCTA CAGGCGCTGC AGGACCCAGA
    CTGTTTACCA TCCATTTAAT AGATGCAAAC ACAGACAACC TTCCGAAAGC CCACACTTGC
    TTTAACCGGA TTGACATTCC GCCTTATGAG TCATATGAGA AGCTTTACGA GAAGCTGTTG
    ACAGCTGTGG AAGAGACATG TGGGTTTGCT GTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_414794.3
    CDS122..2320
    Translation

    Target ORF information:

    RefSeq Version XM_414794.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_414794.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGTTCTA CAGGCGCTGC AGGACCCAGA
    CTGTTTACCA TCCATTTAAT AGATGCAAAC ACAGACAACC TTCCGAAAGC CCACACTTGC
    TTTAACCGGA TTGACATTCC GCCTTATGAG TCATATGAGA AGCTTTACGA GAAGCTGTTG
    ACAGCTGTGG AAGAGACATG TGGGTTTGCT GTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42323
    Clone ID Related Accession (Same CDS sequence) XM_015294375.1 , XM_015294375.2
    Accession Version XM_015294375.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149861.1
    CDS122..2311
    Translation

    Target ORF information:

    RefSeq Version XM_015294375.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42323
    Clone ID Related Accession (Same CDS sequence) XM_015294375.1 , XM_015294375.2
    Accession Version XM_015294375.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149861.1
    CDS129..2318
    Translation

    Target ORF information:

    RefSeq Version XM_015294375.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294375.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACGCG TCGGAACGGC TCCAGCATCA AGATCCGCCT CACAGTATTA 
    TGTGCCAAGA ATCTTGCAAA GAAAGATTTC TTCAGACTTC CTGATCCATT TGCAAAAATA
    GTTGTTGATG GGTCAGGTCA GTGCCACTCA ACTGACACTG TGAAGAACAC GTTGGACCCC
    AAATGGAACC AGCATTATGA TCTGTATGTT GGGAAGACAG ATTCCATAAC CATCAGTGTA
    TGGAACCATA AGAAAATCCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTCCGACTC
    CTCTCCAATG CCATCAGCAG GCTAAAAGAT ACTGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAATCCCA CGGATACTGA TGCTGTACGA GGCCAAATAG TGGTCAGTTT ACAGACACGG
    GACAGAATAG GCACCGGAGG TTCTGTAGTA GACTGTAGAG GGCTTTTGGA GAATGAAGGA
    ACGGTTTATG AAGATTCTGG ACCAGGGAGG CCACTGAGCT GTTTTATGGA AGAACCAGCA
    CCCTACACAG ACACTACAGG GGCTGCAGGT GGAGGCAATT GTCGCTTTGT AGAGTCCCCC
    AGTCAAGATC AGAGACTTCA GGCACAGAGA CTTCGGACTC CTGAAGTCAG AGGTCATGTA
    CAGACACCTC AGAACAGACC ACACGGTCAC CAGTCTCCTG ACCTACCTGA AGGCTATGAA
    CAAAGAACAA CGGTGCAGGG CCAGGTTTAT TTTTTGCACA CGCAGACTGG AGTTAGCACA
    TGGCATGACC CTAGGATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA ACTGGGACCT
    CTGCCTCCAG GTTGGGAAGT TAGAAGTACA GTTTCAGGAA GAATATATTT TGTAGATCAT
    AACAACAGAA CCACGCAGTT CACAGATCCA CGACTACATC ACATCATGAA TCACCAATGC
    CAGCTAAAAG AGCCCAGCCA GCAGCCTCTG CCAGCTCCAA ATGAGGGGTC TGTGGAGGAG
    GGTGAAGAGT TGCCTGCACA GAGATATGAA AGAGACTTGG TGCAAAAGCT GAAAGTTCTT
    AGACATGAGC TCTCCCTTCA ACAACCACAG GCAGGTCACT GCCGAATTGA AGTATCCAGA
    GAGGAAATAT TTGAGGAATC CTACCGTCAG ATAATGAAGA TGAGGCCAAA GGATTTGAAA
    AAGAGGCTTA TGGTAAAATT TCGAGGCGAG GAAGGCCTGG ATTATGGAGG GGTAGCAAGA
    GAATGGTTGT ATTTACTGTG CCATGAAATG TTGAATCCAT ACTATGGACT CTTCCAGTAC
    TCTACAGATA ATATTTACAT GCTGCAAATC AATCCAGACT CCTCTATCAA TCCTGACCAT
    TTGTCTTATT TCCATTTTGT TGGGCGAATA ATGGGTTTGG CTGTGTTCCA TGGACACTAT
    ATCAATGGGG GGTTCACGGT TCCCTTTTAT AAACAGCTCC TGGGGAAACC CATTCAGCTC
    TCTGATCTGG AATCTGTTGA CCCAGAACTA CACAAAAGTT TAGTTTGGAT TCTAGAGAAC
    GATATCACCC CGGTTCTGGA CCATACTTTT TGTGTGGAAC ACAATGCTTT TGGGAGGATT
    TTACAGCACG AACTCAAACC AAATGGCAGG AACATTCCAG TGACAGAAGA GAACAAGAAA
    GAATATGTCA GGTTGTATGT GAACTGGCGA TTTATGAGAG GCATAGAAGC ACAGTTCTTG
    GCTCTGCAGA AGGGTTTTAA CGAACTCATT CCCCAGCATC TCCTCAAGCC TTTTGACCAG
    AAGGAACTTG AGCTAATCAT AGGAGGCCTG GATAAGATAG ACCTGAATGA CTGGAAGTCA
    AACACACGTC TAAAGCACTG CATGGCAGAT AGCAATATTG TGAAGTGGTT CTGGCAAGCG
    GTGGAAACAT TTGATGAAGA AAGAAGGGCA AGGCTTTTGC AGTTTGTGAC AGGTTCAACA
    CGTGTCCCAC TTCAAGGTTT CAAGGCTTTA CAAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42324
    Clone ID Related Accession (Same CDS sequence) XM_015294376.1 , XM_015294376.2
    Accession Version XM_015294376.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGTGTGA CAAATCCACC ACTTATCTAC ATATTATGTG CCAAGAATCT TGCAAAGAAA 
    GATTTCTTCA GACTTCCTGA TCCATTTGCA AAAATAGTTG TTGATGGGTC AGGTCAGTGC
    CACTCAACTG ACACTGTGAA GAACACGTTG GACCCCAAAT GGAACCAGCA TTATGATCTG
    TATGTTGGGA AGACAGATTC CATAACCATC AGTGTATGGA ACCATAAGAA AATCCACAAG
    AAACAGGGAG CTGGCTTCCT GGGGTGTGTC CGACTCCTCT CCAATGCCAT CAGCAGGCTA
    AAAGATACTG GATACCAGCG TTTGGATCTA TGCAAACTAA ATCCCACGGA TACTGATGCT
    GTACGAGGCC AAATAGTGGT CAGTTTACAG ACACGGGACA GAATAGGCAC CGGAGGTTCT
    GTAGTAGACT GTAGAGGGCT TTTGGAGAAT GAAGGTTTCC CCATTTTCAG AACGGTTTAT
    GAAGATTCTG GACCAGGGAG GCCACTGAGC TGTTTTATGG AAGAACCAGC ACCCTACACA
    GACACTACAG GGGCTGCAGG TGGAGGCAAT TGTCGCTTTG TAGAGTCCCC CAGTCAAGAT
    CAGAGACTTC AGGCACAGAG ACTTCGGACT CCTGAAGTCA GAGGTCATGT ACAGACACCT
    CAGAACAGAC CACACGGTCA CCAGTCTCCT GACCTACCTG AAGGCTATGA ACAAAGAACA
    ACGGTGCAGG GCCAGGTTTA TTTTTTGCAC ACGCAGACTG GAGTTAGCAC ATGGCATGAC
    CCTAGGATAC CAAGAGACCT TAACAGTGTG AATTGTGATG AACTGGGACC TCTGCCTCCA
    GGTTGGGAAG TTAGAAGTAC AGTTTCAGGA AGAATATATT TTGTAGATCA TAACAACAGA
    ACCACGCAGT TCACAGATCC ACGACTACAT CACATCATGA ATCACCAATG CCAGCTAAAA
    GAGCCCAGCC AGCAGCCTCT GCCAGCTCCA AATGAGGGGT CTGTGGAGGA GGGTGAAGAG
    TTGCCTGCAC AGAGATATGA AAGAGACTTG GTGCAAAAGC TGAAAGTTCT TAGACATGAG
    CTCTCCCTTC AACAACCACA GGCAGGTCAC TGCCGAATTG AAGTATCCAG AGAGGAAATA
    TTTGAGGAAT CCTACCGTCA GATAATGAAG ATGAGGCCAA AGGATTTGAA AAAGAGGCTT
    ATGGTAAAAT TTCGAGGCGA GGAAGGCCTG GATTATGGAG GGGTAGCAAG AGAATGGTTG
    TATTTACTGT GCCATGAAAT GTTGAATCCA TACTATGGAC TCTTCCAGTA CTCTACAGAT
    AATATTTACA TGCTGCAAAT CAATCCAGAC TCCTCTATCA ATCCTGACCA TTTGTCTTAT
    TTCCATTTTG TTGGGCGAAT AATGGGTTTG GCTGTGTTCC ATGGACACTA TATCAATGGG
    GGGTTCACGG TTCCCTTTTA TAAACAGCTC CTGGGGAAAC CCATTCAGCT CTCTGATCTG
    GAATCTGTTG ACCCAGAACT ACACAAAAGT TTAGTTTGGA TTCTAGAGAA CGATATCACC
    CCGGTTCTGG ACCATACTTT TTGTGTGGAA CACAATGCTT TTGGGAGGAT TTTACAGCAC
    GAACTCAAAC CAAATGGCAG GAACATTCCA GTGACAGAAG AGAACAAGAA AGAATATGTC
    AGGTTGTATG TGAACTGGCG ATTTATGAGA GGCATAGAAG CACAGTTCTT GGCTCTGCAG
    AAGGGTTTTA ACGAACTCAT TCCCCAGCAT CTCCTCAAGC CTTTTGACCA GAAGGAACTT
    GAGCTAATCA TAGGAGGCCT GGATAAGATA GACCTGAATG ACTGGAAGTC AAACACACGT
    CTAAAGCACT GCATGGCAGA TAGCAATATT GTGAAGTGGT TCTGGCAAGC GGTGGAAACA
    TTTGATGAAG AAAGAAGGGC AAGGCTTTTG CAGTTTGTGA CAGGTTCAAC ACGTGTCCCA
    CTTCAAGGTT TCAAGGCTTT ACAAGGTTCT ACAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149862.1
    CDS16..2205
    Translation

    Target ORF information:

    RefSeq Version XM_015294376.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGTGTGA CAAATCCACC ACTTATCTAC ATATTATGTG CCAAGAATCT TGCAAAGAAA 
    GATTTCTTCA GACTTCCTGA TCCATTTGCA AAAATAGTTG TTGATGGGTC AGGTCAGTGC
    CACTCAACTG ACACTGTGAA GAACACGTTG GACCCCAAAT GGAACCAGCA TTATGATCTG
    TATGTTGGGA AGACAGATTC CATAACCATC AGTGTATGGA ACCATAAGAA AATCCACAAG
    AAACAGGGAG CTGGCTTCCT GGGGTGTGTC CGACTCCTCT CCAATGCCAT CAGCAGGCTA
    AAAGATACTG GATACCAGCG TTTGGATCTA TGCAAACTAA ATCCCACGGA TACTGATGCT
    GTACGAGGCC AAATAGTGGT CAGTTTACAG ACACGGGACA GAATAGGCAC CGGAGGTTCT
    GTAGTAGACT GTAGAGGGCT TTTGGAGAAT GAAGGTTTCC CCATTTTCAG AACGGTTTAT
    GAAGATTCTG GACCAGGGAG GCCACTGAGC TGTTTTATGG AAGAACCAGC ACCCTACACA
    GACACTACAG GGGCTGCAGG TGGAGGCAAT TGTCGCTTTG TAGAGTCCCC CAGTCAAGAT
    CAGAGACTTC AGGCACAGAG ACTTCGGACT CCTGAAGTCA GAGGTCATGT ACAGACACCT
    CAGAACAGAC CACACGGTCA CCAGTCTCCT GACCTACCTG AAGGCTATGA ACAAAGAACA
    ACGGTGCAGG GCCAGGTTTA TTTTTTGCAC ACGCAGACTG GAGTTAGCAC ATGGCATGAC
    CCTAGGATAC CAAGAGACCT TAACAGTGTG AATTGTGATG AACTGGGACC TCTGCCTCCA
    GGTTGGGAAG TTAGAAGTAC AGTTTCAGGA AGAATATATT TTGTAGATCA TAACAACAGA
    ACCACGCAGT TCACAGATCC ACGACTACAT CACATCATGA ATCACCAATG CCAGCTAAAA
    GAGCCCAGCC AGCAGCCTCT GCCAGCTCCA AATGAGGGGT CTGTGGAGGA GGGTGAAGAG
    TTGCCTGCAC AGAGATATGA AAGAGACTTG GTGCAAAAGC TGAAAGTTCT TAGACATGAG
    CTCTCCCTTC AACAACCACA GGCAGGTCAC TGCCGAATTG AAGTATCCAG AGAGGAAATA
    TTTGAGGAAT CCTACCGTCA GATAATGAAG ATGAGGCCAA AGGATTTGAA AAAGAGGCTT
    ATGGTAAAAT TTCGAGGCGA GGAAGGCCTG GATTATGGAG GGGTAGCAAG AGAATGGTTG
    TATTTACTGT GCCATGAAAT GTTGAATCCA TACTATGGAC TCTTCCAGTA CTCTACAGAT
    AATATTTACA TGCTGCAAAT CAATCCAGAC TCCTCTATCA ATCCTGACCA TTTGTCTTAT
    TTCCATTTTG TTGGGCGAAT AATGGGTTTG GCTGTGTTCC ATGGACACTA TATCAATGGG
    GGGTTCACGG TTCCCTTTTA TAAACAGCTC CTGGGGAAAC CCATTCAGCT CTCTGATCTG
    GAATCTGTTG ACCCAGAACT ACACAAAAGT TTAGTTTGGA TTCTAGAGAA CGATATCACC
    CCGGTTCTGG ACCATACTTT TTGTGTGGAA CACAATGCTT TTGGGAGGAT TTTACAGCAC
    GAACTCAAAC CAAATGGCAG GAACATTCCA GTGACAGAAG AGAACAAGAA AGAATATGTC
    AGGTTGTATG TGAACTGGCG ATTTATGAGA GGCATAGAAG CACAGTTCTT GGCTCTGCAG
    AAGGGTTTTA ACGAACTCAT TCCCCAGCAT CTCCTCAAGC CTTTTGACCA GAAGGAACTT
    GAGCTAATCA TAGGAGGCCT GGATAAGATA GACCTGAATG ACTGGAAGTC AAACACACGT
    CTAAAGCACT GCATGGCAGA TAGCAATATT GTGAAGTGGT TCTGGCAAGC GGTGGAAACA
    TTTGATGAAG AAAGAAGGGC AAGGCTTTTG CAGTTTGTGA CAGGTTCAAC ACGTGTCCCA
    CTTCAAGGTT TCAAGGCTTT ACAAGGTTCT ACAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42324
    Clone ID Related Accession (Same CDS sequence) XM_015294376.1 , XM_015294376.2
    Accession Version XM_015294376.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGTGTGA CAAATCCACC ACTTATCTAC ATATTATGTG CCAAGAATCT TGCAAAGAAA 
    GATTTCTTCA GACTTCCTGA TCCATTTGCA AAAATAGTTG TTGATGGGTC AGGTCAGTGC
    CACTCAACTG ACACTGTGAA GAACACGTTG GACCCCAAAT GGAACCAGCA TTATGATCTG
    TATGTTGGGA AGACAGATTC CATAACCATC AGTGTATGGA ACCATAAGAA AATCCACAAG
    AAACAGGGAG CTGGCTTCCT GGGGTGTGTC CGACTCCTCT CCAATGCCAT CAGCAGGCTA
    AAAGATACTG GATACCAGCG TTTGGATCTA TGCAAACTAA ATCCCACGGA TACTGATGCT
    GTACGAGGCC AAATAGTGGT CAGTTTACAG ACACGGGACA GAATAGGCAC CGGAGGTTCT
    GTAGTAGACT GTAGAGGGCT TTTGGAGAAT GAAGGTTTCC CCATTTTCAG AACGGTTTAT
    GAAGATTCTG GACCAGGGAG GCCACTGAGC TGTTTTATGG AAGAACCAGC ACCCTACACA
    GACACTACAG GGGCTGCAGG TGGAGGCAAT TGTCGCTTTG TAGAGTCCCC CAGTCAAGAT
    CAGAGACTTC AGGCACAGAG ACTTCGGACT CCTGAAGTCA GAGGTCATGT ACAGACACCT
    CAGAACAGAC CACACGGTCA CCAGTCTCCT GACCTACCTG AAGGCTATGA ACAAAGAACA
    ACGGTGCAGG GCCAGGTTTA TTTTTTGCAC ACGCAGACTG GAGTTAGCAC ATGGCATGAC
    CCTAGGATAC CAAGAGACCT TAACAGTGTG AATTGTGATG AACTGGGACC TCTGCCTCCA
    GGTTGGGAAG TTAGAAGTAC AGTTTCAGGA AGAATATATT TTGTAGATCA TAACAACAGA
    ACCACGCAGT TCACAGATCC ACGACTACAT CACATCATGA ATCACCAATG CCAGCTAAAA
    GAGCCCAGCC AGCAGCCTCT GCCAGCTCCA AATGAGGGGT CTGTGGAGGA GGGTGAAGAG
    TTGCCTGCAC AGAGATATGA AAGAGACTTG GTGCAAAAGC TGAAAGTTCT TAGACATGAG
    CTCTCCCTTC AACAACCACA GGCAGGTCAC TGCCGAATTG AAGTATCCAG AGAGGAAATA
    TTTGAGGAAT CCTACCGTCA GATAATGAAG ATGAGGCCAA AGGATTTGAA AAAGAGGCTT
    ATGGTAAAAT TTCGAGGCGA GGAAGGCCTG GATTATGGAG GGGTAGCAAG AGAATGGTTG
    TATTTACTGT GCCATGAAAT GTTGAATCCA TACTATGGAC TCTTCCAGTA CTCTACAGAT
    AATATTTACA TGCTGCAAAT CAATCCAGAC TCCTCTATCA ATCCTGACCA TTTGTCTTAT
    TTCCATTTTG TTGGGCGAAT AATGGGTTTG GCTGTGTTCC ATGGACACTA TATCAATGGG
    GGGTTCACGG TTCCCTTTTA TAAACAGCTC CTGGGGAAAC CCATTCAGCT CTCTGATCTG
    GAATCTGTTG ACCCAGAACT ACACAAAAGT TTAGTTTGGA TTCTAGAGAA CGATATCACC
    CCGGTTCTGG ACCATACTTT TTGTGTGGAA CACAATGCTT TTGGGAGGAT TTTACAGCAC
    GAACTCAAAC CAAATGGCAG GAACATTCCA GTGACAGAAG AGAACAAGAA AGAATATGTC
    AGGTTGTATG TGAACTGGCG ATTTATGAGA GGCATAGAAG CACAGTTCTT GGCTCTGCAG
    AAGGGTTTTA ACGAACTCAT TCCCCAGCAT CTCCTCAAGC CTTTTGACCA GAAGGAACTT
    GAGCTAATCA TAGGAGGCCT GGATAAGATA GACCTGAATG ACTGGAAGTC AAACACACGT
    CTAAAGCACT GCATGGCAGA TAGCAATATT GTGAAGTGGT TCTGGCAAGC GGTGGAAACA
    TTTGATGAAG AAAGAAGGGC AAGGCTTTTG CAGTTTGTGA CAGGTTCAAC ACGTGTCCCA
    CTTCAAGGTT TCAAGGCTTT ACAAGGTTCT ACAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149862.1
    CDS16..2205
    Translation

    Target ORF information:

    RefSeq Version XM_015294376.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294376.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGTGTGA CAAATCCACC ACTTATCTAC ATATTATGTG CCAAGAATCT TGCAAAGAAA 
    GATTTCTTCA GACTTCCTGA TCCATTTGCA AAAATAGTTG TTGATGGGTC AGGTCAGTGC
    CACTCAACTG ACACTGTGAA GAACACGTTG GACCCCAAAT GGAACCAGCA TTATGATCTG
    TATGTTGGGA AGACAGATTC CATAACCATC AGTGTATGGA ACCATAAGAA AATCCACAAG
    AAACAGGGAG CTGGCTTCCT GGGGTGTGTC CGACTCCTCT CCAATGCCAT CAGCAGGCTA
    AAAGATACTG GATACCAGCG TTTGGATCTA TGCAAACTAA ATCCCACGGA TACTGATGCT
    GTACGAGGCC AAATAGTGGT CAGTTTACAG ACACGGGACA GAATAGGCAC CGGAGGTTCT
    GTAGTAGACT GTAGAGGGCT TTTGGAGAAT GAAGGTTTCC CCATTTTCAG AACGGTTTAT
    GAAGATTCTG GACCAGGGAG GCCACTGAGC TGTTTTATGG AAGAACCAGC ACCCTACACA
    GACACTACAG GGGCTGCAGG TGGAGGCAAT TGTCGCTTTG TAGAGTCCCC CAGTCAAGAT
    CAGAGACTTC AGGCACAGAG ACTTCGGACT CCTGAAGTCA GAGGTCATGT ACAGACACCT
    CAGAACAGAC CACACGGTCA CCAGTCTCCT GACCTACCTG AAGGCTATGA ACAAAGAACA
    ACGGTGCAGG GCCAGGTTTA TTTTTTGCAC ACGCAGACTG GAGTTAGCAC ATGGCATGAC
    CCTAGGATAC CAAGAGACCT TAACAGTGTG AATTGTGATG AACTGGGACC TCTGCCTCCA
    GGTTGGGAAG TTAGAAGTAC AGTTTCAGGA AGAATATATT TTGTAGATCA TAACAACAGA
    ACCACGCAGT TCACAGATCC ACGACTACAT CACATCATGA ATCACCAATG CCAGCTAAAA
    GAGCCCAGCC AGCAGCCTCT GCCAGCTCCA AATGAGGGGT CTGTGGAGGA GGGTGAAGAG
    TTGCCTGCAC AGAGATATGA AAGAGACTTG GTGCAAAAGC TGAAAGTTCT TAGACATGAG
    CTCTCCCTTC AACAACCACA GGCAGGTCAC TGCCGAATTG AAGTATCCAG AGAGGAAATA
    TTTGAGGAAT CCTACCGTCA GATAATGAAG ATGAGGCCAA AGGATTTGAA AAAGAGGCTT
    ATGGTAAAAT TTCGAGGCGA GGAAGGCCTG GATTATGGAG GGGTAGCAAG AGAATGGTTG
    TATTTACTGT GCCATGAAAT GTTGAATCCA TACTATGGAC TCTTCCAGTA CTCTACAGAT
    AATATTTACA TGCTGCAAAT CAATCCAGAC TCCTCTATCA ATCCTGACCA TTTGTCTTAT
    TTCCATTTTG TTGGGCGAAT AATGGGTTTG GCTGTGTTCC ATGGACACTA TATCAATGGG
    GGGTTCACGG TTCCCTTTTA TAAACAGCTC CTGGGGAAAC CCATTCAGCT CTCTGATCTG
    GAATCTGTTG ACCCAGAACT ACACAAAAGT TTAGTTTGGA TTCTAGAGAA CGATATCACC
    CCGGTTCTGG ACCATACTTT TTGTGTGGAA CACAATGCTT TTGGGAGGAT TTTACAGCAC
    GAACTCAAAC CAAATGGCAG GAACATTCCA GTGACAGAAG AGAACAAGAA AGAATATGTC
    AGGTTGTATG TGAACTGGCG ATTTATGAGA GGCATAGAAG CACAGTTCTT GGCTCTGCAG
    AAGGGTTTTA ACGAACTCAT TCCCCAGCAT CTCCTCAAGC CTTTTGACCA GAAGGAACTT
    GAGCTAATCA TAGGAGGCCT GGATAAGATA GACCTGAATG ACTGGAAGTC AAACACACGT
    CTAAAGCACT GCATGGCAGA TAGCAATATT GTGAAGTGGT TCTGGCAAGC GGTGGAAACA
    TTTGATGAAG AAAGAAGGGC AAGGCTTTTG CAGTTTGTGA CAGGTTCAAC ACGTGTCCCA
    CTTCAAGGTT TCAAGGCTTT ACAAGGTTCT ACAGGCGCTG CAGGACCCAG ACTGTTTACC
    ATCCATTTAA TAGATGCAAA CACAGACAAC CTTCCGAAAG CCCACACTTG CTTTAACCGG
    ATTGACATTC CGCCTTATGA GTCATATGAG AAGCTTTACG AGAAGCTGTT GACAGCTGTG
    GAAGAGACAT GTGGGTTTGC TGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.